Membrane pushing device of blood membrane smear for plasmodium detection

Abstract

The utility model discloses a film pushing device of a blood film smear for plasmodium detection. The film pushing device comprises a bottom base, a rising and falling control unit vertically arranged on one side edge of the bottom base, and a combined film pushing unit fixedly connected with the output end of the rising and falling control unit in the transverse direction. A tongue-shaped blood membrane pushing piece assembly and a round blood membrane pushing piece assembly are systematically integrated, and a visual camera is combined to identify blood drop and blood membrane position shape features, so that a combined pushing piece unit is guided and controlled; and meanwhile, a rising and falling control unit is integrated, so that the blood diaphragm is convenient to mount and push, high-automation operation is realized, and the convenience and high efficiency of blood diaphragm manufacturing are improved.

Description

Technical Field

[0001] The embodiments of the present utility model belong to the technical field of blood film spreading technology, and more specifically, relate to a film spreading device for blood films used in malaria parasite detection. Background Art

[0002] The blood smear microscopy method is the gold standard for malaria parasite detection, and there are specific regulations on blood smear microscopy in "Diagnosis of Malaria" WS259-2015 and "Blood Smear Microscopy Method for Malaria Parasite Detection" WS / T 569—2017. Currently, the production of blood smears is carried out in accordance with "Blood Smear Microscopy Method for Malaria Parasite Detection" WS / T 569—2017, requiring the simultaneous production of a round thick blood film and a tongue-shaped thin blood film on a single thin slice. Among them, the thick blood film is required to be applied to the left of the center of the glass slide and painted into a circular film with a diameter of 0.8 cm to 1.0 cm, and the thickness should be such that 5 to 10 white blood cells can be seen within one oil immersion field of view. The thin blood film requires the lower edge of the spreader to be flat against the midline of the glass slide. When the blood spreads to about 2 cm wide between the glass slide and the spreader, the two glass slides are kept at 25° to 35°, and quickly pushed forward from right to left to form a tongue-shaped thin blood film. To facilitate the production of the above standard blood films, the development of a blood film spreader device for malaria parasite detection is of great significance for improving the detection efficiency and quality.

[0003] In order to achieve the above technical objectives, the Chinese utility model patent CN202446096U discloses a blood film spreader, which includes a spreader, and there are recesses for easy holding in the middle of both sides of the spreader. This new type of blood film spreader (1) is made of stainless steel, overcoming the disadvantage of the fragile glass material currently used in clinics; (2) adding a middle recess design, which conforms to the curve of the human finger, is more user-friendly, makes the operator hold the spreader more firmly and comfortably, and thus can produce better blood film sheets; (3) both ends of this spreader are designed with smooth grinding and can be used at both ends; (4) this spreader is suitable for a variety of disinfection methods, such as high-pressure steam method, soaking in instrument disinfectant solution, alcohol disinfection, etc.; in addition, the Chinese invention patent CN107300495A discloses a composite stain for piroplasm blood smear, including composite stain I and composite stain II; the composite stain I is composed of 2 g of Wright's powder, 1.7 g of Giemsa pigment, 50 ml of glycerin and 1000 ml of methanol, and the composite stain II is composed of 2.3 g of anhydrous sodium dihydrogen phosphate, 0.3 g of anhydrous potassium dihydrogen phosphate and 1000 ml of distilled water. The staining method includes the following steps: 1. Preparation of blood smear; 2. Secondary treatment of blood smear; 3. Staining in the composite stain. The composite dye and staining method of the present invention have good staining effect on piroplasm without the need to rely on high-end instrument equipment, are economical and practical, require a short time, and there is no impurity interference in observing the parasite body, and the effect is extremely prominent.

[0004] The above-mentioned patented technologies have been designed and optimized respectively from the aspects of the shape of the spreading piece and the production of tongue-shaped blood films, but there are still the following technical problems or areas for improvement: (1) The required blood film shapes in the malaria parasite detection standard are circular thick blood films and tongue-shaped thin blood films, but there is a technical gap in the existing technology for the automatic production of circular thick blood films; (2) The production technologies of circular thick blood films and tongue-shaped thin blood films should be advanced towards system integration to improve the efficiency of the production of malaria parasite detection blood films; (3) On the basis of (2), the blood film production system should be further optimized towards automation to improve the convenience during the operation process. Utility Model Content

[0005] In view of the above defects or improvement requirements of the existing technology, the present utility model provides a film spreading device for blood films used in malaria parasite detection. By systematically integrating the tongue-shaped blood film spreading component and the circular blood film spreading component, and combining the visual camera to identify the position and shape characteristics of the blood droplet and the blood film to guide and control the combined spreading unit; at the same time, an integrated lifting control unit is integrated to facilitate the installation and film spreading of the blood film, and highly automated operation is realized, improving the convenience and efficiency of the production of blood films.

[0006] To achieve the above object, a film spreading device for blood films used in malaria parasite detection includes:

[0007] A bottom base, a lifting control unit vertically arranged on one side of the bottom base, and a combined spreading unit horizontally fixed to the output end of the lifting control unit;

[0008] The bottom base includes a slide groove on the upper surface for installing the blood film, and a pressing component arranged on the side of the slide groove;

[0009] The lifting control unit includes a side column and a lifting control component arranged inside the side column for outputting vertical lifting movement, and the output end of the lifting control component is fixedly connected to the combined spreading unit;

[0010] The combined spreading unit includes a housing horizontally fixed to the output end of the lifting control component, a tongue-shaped blood film spreading component and a circular blood film spreading component arranged side by side on the lower surface of the housing; the circular blood film spreading component includes a second spreading driving motor fixedly connected to the housing, a second spreading driving lead screw coaxially and fixedly connected to the second spreading driving motor, a third nut block in threaded linear transmission with the second spreading driving lead screw, a spreading execution component fixed below the third nut block, and a visual camera arranged on the bottom surface of the housing.

[0011] Preferably, the lifting control component includes:

[0012] The lifting control motor fixedly connected to the side column, the lifting control lead screw fixedly connected coaxially with the output shaft of the lifting control motor, and the first nut block in threaded linear transmission with the lifting control lead screw, wherein the housing is fixedly connected to the first nut block.

[0013] Preferably, the tongue-shaped blood film spreading assembly includes:

[0014] The first spreading driving motor fixedly connected to the housing, the first spreading driving lead screw fixedly connected coaxially with the first spreading driving motor, the second nut block in threaded linear transmission with the first spreading driving lead screw, the first connecting rod fixedly connected to the bottom surface of the second nut block and adjustable in telescopic manner, and the bottom spreading knife with an angle-adjustable connection at the lower end of the first connecting rod.

[0015] Preferably, the spreading execution assembly is a non-contact spreading assembly, which includes:

[0016] The second connecting rod fixedly connected to the lower surface of the third nut block, the air jet channel coaxially penetrating through the second connecting rod, and the air inlet pipe communicating with the upper end of the air jet channel.

[0017] Preferably, the non-contact spreading assembly further includes: a splash guard fixed to the lower end of the second connecting rod.

[0018] Preferably, the spreading execution assembly is a contact spreading assembly, which includes:

[0019] The rotating motor fixedly connected to the lower surface of the third nut block in the vertical direction, the third connecting rod fixedly connected coaxially with the output shaft of the rotating motor, and the L-shaped spreading knife fixed horizontally at the lower end of the third connecting rod; the L-shaped spreading knife includes a diameter control rod for ensuring the diameter of the circular thick blood film and a rod end flange fixed at the end of the diameter control rod for preventing blood from overflowing during the blood spreading process.

[0020] Preferably, the pressing assembly includes:

[0021] A pressing block, a side through hole penetrating through one side of the pressing block, and a rotating positioning rod sleeved in the side through hole and rotatably positioning the pressing block on the bottom base.

[0022] Generally speaking, compared with the prior art by the above technical solutions conceived by the present utility model, the following beneficial effects can be achieved:

[0023] (1) A blood film spreading device for malaria parasite detection of the present utility model systematically integrates a tongue-shaped blood film spreading component and a circular blood film spreading component, and combines a vision camera to identify the position and shape characteristics of blood droplets and blood films, guiding and controlling a combined spreading unit; at the same time, an integrated lifting control unit is integrated to facilitate the installation and spreading of blood film slides, and realizes highly automated operation, improving the convenience and efficiency of blood film slide production;

[0024] (2) A blood film spreading device for malaria parasite detection of the present utility model designs two embodiments of a pneumatic non-contact spreading component and a rod-pushing contact spreading component, and combines a vision camera to control the air pressure or the height of an L-shaped pushing knife, so as to effectively realize the precise automatic control of the diameter and thickness of a circular blood film, and effectively realize the standardization of the processing and production of a malaria parasite circular thick blood film. Description of the Drawings

[0025] Figure 1 is a schematic diagram of the overall structure of a blood film spreading device for malaria parasite detection of an embodiment of the present utility model;

[0026] Figure 2 is the second embodiment of the circular thick blood film spreading component of a blood film spreading device for malaria parasite detection of an embodiment of the present utility model;

[0027] Figure 3 is an end top view of the second embodiment of the circular thick blood film spreading component of a blood film spreading device for malaria parasite detection of an embodiment of the present utility model;

[0028] Figure 4 is a blood film slide used in a blood film spreading device for malaria parasite detection of an embodiment of the present utility model;

[0029] Figure 5 is a partial enlarged view A of a blood film spreading device for malaria parasite detection of an embodiment of the present utility model;

[0030] In all the drawings, the same reference numerals represent the same technical features, specifically:

[0031] 1 - Bottom base, 100 - Slide slot, 110 - Tablet pressing assembly, 111 - Pressing block, 112 - Side through - hole, 113 - Rotating positioning rod, 2 - Lifting control unit, 200 - Side column, 210 - Lifting control assembly, 211 - Lifting control motor, 212 - Lifting control lead screw, 213 - First nut block, 3 - Combined slide - pushing unit, 300 - Housing, 310 - Tongue - shaped blood film slide - pushing assembly, 311 - First slide - pushing drive motor, 312 - First slide - pushing drive lead screw, 313 - Second nut block, 314 - First connecting rod, 315 - Bottom slide - pushing knife, 320 - Circular blood film slide - pushing assembly, 321 - Second slide - pushing drive motor, 322 - Second slide - pushing drive lead screw, 323 - Third nut block, 324 - Second connecting rod, 3240 - Jet channel, 325 - Splash guard, 326 - Air inlet pipe, 327 - Rotating motor, 328 - Third connecting rod, 329 - L - shaped push knife, 3291 - Diameter control rod, 3292 - Rod - end flange, 4 - Blood film, 410 - First blood droplet, 411 - Tongue - shaped thin blood film, 420 - Second blood droplet, 421 - Circular thick blood film. Detailed implementation mode

[0032] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model.

[0033] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, "a plurality" means two or more, unless otherwise specifically defined.

[0034] In the present application, unless otherwise clearly specified and limited, the terms "installation", "connection", "connection", "fixation", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0035] In order to make the objectives, technical solutions and advantages of the present utility model clearer and more understandable, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model. In addition, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0036] As Figure 1 shown, in the embodiment of the present utility model, a film pushing device for a blood film smear for malaria parasite detection includes:

[0037] a bottom base 1, a lifting control unit 2 vertically arranged on one side of the bottom base 1, and a combined film pushing unit 3 horizontally and fixedly connected to the output end of the lifting control unit 2;

[0038] The bottom base 1 includes a slide groove 100 opened on the upper surface for installing a blood film, and a film pressing assembly 110 arranged on the side of the slide groove 100;

[0039] The lifting control unit 2 includes a side column 200 and a lifting control assembly 210 arranged inside the side column 200 for outputting a vertical lifting motion. The output end of the lifting control assembly 210 is fixedly connected to the combined film pushing unit 3;

[0040] The combined film pushing unit 3 includes a housing 300 horizontally and fixedly connected to the output end of the lifting control assembly 210, a tongue-shaped blood film pushing assembly 310 and a circular blood film pushing assembly 320 arranged side by side on the lower surface of the housing 300; the circular blood film pushing assembly 320 includes a second film pushing driving motor 321 fixedly connected to the housing 300, a second film pushing driving lead screw 322 coaxially and fixedly connected to the second film pushing driving motor 321, a third nut block 323 in threaded linear transmission with the second film pushing driving lead screw 322, a film pushing execution assembly fixed below the third nut block 323, and a vision camera 330 arranged on the bottom surface of the housing 300.

[0041] Working principle in the embodiment of the present utility model: First, place the blood film piece 4 with a blood droplet dropped thereon into the slide groove 100 and fix it through the pressing component 110; immediately afterwards, adjust the postures of the ends of the tongue-shaped blood film pushing component 310 and the circular blood film pushing component 320, start the lifting control unit 4, and lower the combined pushing unit 3 as a whole to an appropriate height; immediately afterwards, start the tongue-shaped blood film pushing component 310 and the circular blood film pushing component 320 to make the blood droplet into a blood film with a fixed thickness and shape; immediately afterwards, start the lifting control unit 4 in the reverse direction to remove the blood film piece 4, and complete one blood film processing. The ends of the tongue-shaped blood film pushing component 310 and the circular blood film pushing component 320 are made of hydrophobic materials to prevent cross-infection during the blood film processing.

[0042] In the embodiment of the present utility model, by systematically integrating the tongue-shaped blood film pushing component 310 and the circular blood film pushing component 320, and combining the visual camera 330 to identify the position and shape characteristics of the blood droplet and the blood film to guide and control the combined pushing unit; at the same time, the lifting control unit is integrated to facilitate the installation and film pushing of the blood film piece, and realizes highly automated operation, improving the convenience and efficiency of the production of the blood film piece.

[0043] As Figure 1 shown, in the embodiment of the present utility model, the lifting control component 210 includes:

[0044] A lifting control motor 211 fixedly connected to the side column 200, a lifting control lead screw 212 fixedly connected coaxially with the output shaft of the lifting control motor 211, a first nut block 213 in threaded linear transmission with the lifting control lead screw 212, and the housing 300 is fixedly connected to the first nut block 213.

[0045] As Figure 1 and Figure 4 shown, in the embodiment of the present utility model, the tongue-shaped blood film pushing component 310 includes:

[0046] A first pushing driving motor 311 fixedly connected to the housing 300, a first pushing driving lead screw 312 fixedly connected coaxially with the first pushing driving motor 311, a second nut block 313 in threaded linear transmission with the first pushing driving lead screw 312, a first connecting rod 314 fixedly connected to the bottom surface of the second nut block 313 and adjustable in length, and a bottom pushing blade 315 connected to the lower end of the first connecting rod 314 with an adjustable angle.

[0047] As Figure 1 and Figure 4 shown, in the embodiment of the present utility model, the pushing execution component is a non-contact pushing component, which includes:

[0048] The second connecting rod 324 fixedly connected to the lower surface of the third nut block 323, the jet channel 3240 coaxially penetrating through the second connecting rod 324, and the air inlet pipe 326 connected to the upper end of the jet channel 3240.

[0049] As Figure 1 and Figure 4 shown, in the embodiment of the present utility model, the non-contact push sheet assembly further includes: a splash guard 325 fixed to the lower end of the second connecting rod 324.

[0050] As Figure 2 , 3 and Figure 4 shown, in the embodiment of the present utility model, the push sheet execution assembly is a contact push sheet assembly, which includes:

[0051] A rotating motor 327 fixedly connected to the lower surface of the third nut block 323 in a vertical direction, a third connecting rod 328 fixedly connected coaxially with the output shaft of the rotating motor 327, and an L-shaped push knife 329 fixed to the lower end of the third connecting rod 328 in a horizontal direction; the L-shaped push knife 329 includes a diameter control rod 3291 for ensuring the diameter of the circular thick blood film and a rod end flange 3292 fixed to the end of the diameter control rod 3291 and used to prevent blood from overflowing during the blood pushing process.

[0052] In the embodiment of the present utility model, by designing two embodiments of a pneumatic non-contact push sheet assembly and a rod push contact push sheet assembly, combined with a vision camera, the air pressure or the height of the L-shaped push knife 329 can be controlled, and thus the precise automatic control of the diameter and thickness of the circular blood film can be effectively achieved, effectively realizing the standardization of the processing and production of the circular thick blood film of malaria parasites.

[0053] As Figure 5 shown, in the embodiment of the present utility model, the pressing sheet assembly 110 includes:

[0054] A pressing block 111, a side through hole 112 penetrating through one side of the pressing block 111, and a rotating positioning rod 113 sleeved in the side through hole 112 and rotatably positioning the pressing block 111 on the bottom base 1.

[0055] The above are only the preferred embodiments of the present application, and are not intended to limit the present application. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present application shall be included in the protection scope of the present application. The above is only the preferred implementation manner of the present application. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the technical principle of the present application, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present application.

Claims

1. A blood film spreading device for malaria parasite detection, characterized in that, Comprising: A bottom base (1), a lifting control unit (2) vertically arranged on one side of the bottom base (1), and a combined push piece unit (3) horizontally fixed to the output end of the lifting control unit (2); The bottom base (1) includes a slide slot (100) opened on the upper surface for mounting a blood film, and a pressing piece assembly (110) arranged on the side of the slide slot (100); The lifting control unit (2) includes a side column (200) and a lifting control assembly (210) arranged inside the side column (200) for outputting vertical lifting motion. The output end of the lifting control assembly (210) is fixedly connected to the combined push piece unit (3); The combined push piece unit (3) includes a housing (300) horizontally fixed to the output end of the lifting control assembly (210), a tongue-shaped blood film push piece assembly (310) and a circular blood film push piece assembly (320) arranged side by side on the lower surface of the housing (300); The circular blood film push piece assembly (320) includes a second push piece driving motor (321) fixedly connected to the housing (300), a second push piece driving screw rod (322) coaxially and fixedly connected to the second push piece driving motor (321), a third nut block (323) in threaded linear transmission with the second push piece driving screw rod (322), a push piece execution assembly fixed below the third nut block (323), and a vision camera (330) arranged on the bottom surface of the housing (300).

2. The blood film spreading device for malaria parasite detection according to claim 1, characterized in that, The lifting control assembly (210) includes: A lifting control motor (211) fixedly connected to the side column (200), a lifting control screw rod (212) coaxially and fixedly connected to the output shaft of the lifting control motor (211), and a first nut block (213) in threaded linear transmission with the lifting control screw rod (212). The housing (300) is fixedly connected to the first nut block (213).

3. The blood film spreading device for malaria parasite detection according to claim 1, wherein, The tongue-shaped blood film push piece assembly (310) includes: A first push piece driving motor (311) fixedly connected to the housing (300), a first push piece driving screw rod (312) coaxially and fixedly connected to the first push piece driving motor (311), a second nut block (313) in threaded linear transmission with the first push piece driving screw rod (312), a first connecting rod (314) fixedly connected to the bottom surface of the second nut block (313) and capable of telescopic adjustment, and a bottom push piece knife (315) with an angle-adjustable connection at the lower end of the first connecting rod (314).

4. A blood film spreading device for malaria parasite detection according to claim 1, characterized in that The push piece execution assembly is a non-contact push piece assembly, which includes: A second connecting rod (324) fixedly connected to the lower surface of the third nut block (323), an air jet channel (3240) coaxially penetrating through the second connecting rod (324), and an air inlet pipe (326) communicating with the upper end of the air jet channel (3240).

5. A spreading device for blood film smears for malaria parasite detection according to claim 4, characterized in that, The non-contact push piece assembly further includes a splash guard (325) fixed to the lower end of the second connecting rod (324).

6. The blood film spreading device for malaria parasite detection according to claim 1, characterized in that, The slide pushing execution component is a contact type slide pushing component, which includes: A rotating motor (327) fixedly connected to the lower surface of the third nut block (323) vertically, a third connecting rod (328) fixedly connected coaxially with the output shaft of the rotating motor (327), and an L-shaped push knife (329) fixed horizontally to the lower end of the third connecting rod (328); the L-shaped push knife (329) includes a diameter control rod (3291) for ensuring the diameter of the circular thick blood smear and a rod end flange (3292) fixed to the end of the diameter control rod (3291) and used to prevent blood from overflowing during the blood smear process.

7. A blood film spreading device for malaria parasite detection according to any one of claims 1 to 6, characterized in that, The pressing component (110) includes: A pressing block (111), a side through hole (112) provided through one side of the pressing block (111), and a rotating positioning rod (113) sleeved in the side through hole (112) and rotatably positioning the pressing block (111) axially on the bottom base (1).

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