Nested peripheral blood leucocyte separation column

Abstract

The utility model discloses a nested peripheral blood leukocyte separation column which comprises a tube body and a cover body, and the cover body is connected with the tube body; wherein the pipe body comprises a first pipe body and a second pipe body, the first pipe body is located in the second pipe body, the first pipe body is provided with a first injection port and a separating screen, the first injection port and the separating screen are arranged at the two opposite ends of the first pipe body respectively, a circulation channel is formed in the second pipe body, and the first injection port and the separating screen are communicated through the circulation channel. The circulation channel is communicated with the separating screen, the second pipe body is provided with a second injection port and an output port, and the second injection port and the output port are communicated with the two opposite ends of the circulation channel respectively.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and more specifically, to a nested peripheral blood leukocyte separation column. Background Art

[0002] With the continuous improvement of the needs of scientific research, the requirements for cell separation technology are also constantly increasing. Many scientific researches need to conduct functional studies on leukocytes in peripheral blood to detect cytokines, cell functions, etc.

[0003] However, the current cell separation and purification methods are based on the physical or biochemical characteristics of cells, but there are still certain drawbacks, such as cumbersome operation, high cost, etc. The ammonium chloride separation method and other methods that lyse red blood cells are economically simple, but the interference of red blood cells and debris often leads to unsatisfactory purity, and it is impossible to obtain the vast majority of leukocytes other than red blood cells.

[0004] Therefore, a new technical solution is needed to solve the above technical problems. Summary of the Utility Model

[0005] An object of the present application is to provide a new technical solution for a nested peripheral blood leukocyte separation column.

[0006] According to one aspect of the present application, a nested peripheral blood leukocyte separation column is provided. The nested peripheral blood leukocyte separation column includes a tube body and a cover body, and the cover body is connected to the tube body; wherein, the tube body includes a first tube body and a second tube body, the first tube body is located inside the second tube body, the first tube body has a first injection port and a separation sieve, the first injection port and the separation sieve are respectively arranged at opposite ends of the first tube body, a flow passage is arranged inside the second tube body, the flow passage is communicated with the separation sieve, the second tube body has a second injection port and an output port, and the second injection port and the output port are respectively communicated with opposite ends of the flow passage.

[0007] Optionally, the cover body can be detachably connected to the first tube body and the second tube body respectively.

[0008] Optionally, the cover body has an injection port, a first connecting member and a second connecting member, the first connecting member is located inside the second connecting member, the first connecting member is connected to the first tube body, the second connecting member is connected to the second tube body, the injection port is arranged on the first connecting member, and the injection port is communicated with the first injection port.

[0009] Optionally, the cover body, the first tube body, and the second tube body are all connected by threads. The first connecting member is provided with a first internal thread, and the end of the first tube body near the first injection port is provided with a first external thread, and the first internal thread and the first external thread are matched; the second connecting member is provided with a second internal thread, and the end of the second tube body near the second injection port is provided with a second external thread, and the second internal thread and the second external thread are matched.

[0010] Optionally, the flow passage has a liquid through hole, the liquid through hole is arranged between the second injection port and the output port, the liquid through hole is located at one end of the second tube body away from the cover body, and the liquid through hole is arranged corresponding to the separation sieve.

[0011] Optionally, the tube body has a first end and a second end arranged oppositely, and the cover body is respectively connected to the first ends of the first tube body and the second tube body; in the state where the cover body is respectively connected to the first tube body and the second tube body, a separation cavity is formed between the second end of the first tube body and the second end of the second tube body, and the separation cavity is respectively communicated with the separation sieve and the liquid through hole.

[0012] Optionally, the nested peripheral blood leukocyte separation column further includes an infrared sensor, the infrared sensor is arranged on the tube body, and the infrared sensor is used to monitor the liquid level between the separation sieve and the liquid through hole.

[0013] Optionally, the nested peripheral blood leukocyte separation column further includes a flow controller, the flow controller is connected to the second injection port, and the flow controller is used to control the liquid flow rate of the second injection port.

[0014] Optionally, the nested peripheral blood leukocyte separation column further includes a waste liquid collector, the waste liquid collector is communicated with the output port, and the waste liquid collector is used to store the waste liquid discharged from the flow passage.

[0015] In the embodiment of the present application, the sample is lysed by the first tube body and the red blood cell fragments are screened out, effectively improving the peripheral blood leukocyte capture ability. The cleaning liquid is transported through the flow passage of the second tube body to drive the lysed red blood cell fragments to output from the second tube body through the cleaning liquid, effectively improving the separation purity of the cells, and the recovery rate also increases accordingly. Moreover, through the combined use of the cover body, the first tube body, and the second tube body, the operation of the separation column is simple and the overall cost is relatively low, which is convenient for popularization and application.

[0016] Other features and advantages of the present application will become clear through the following detailed description of the exemplary embodiments of the present application with reference to the accompanying drawings. Description of the Drawings

[0017] The accompanying drawings incorporated in and forming a part of this specification illustrate embodiments of the present application and, together with the description thereof, serve to explain the principles of the present application.

[0018] Figure 1 It is a schematic diagram of the usage state of the separation column in an embodiment of the present application;

[0019] Figure 2 It is a schematic cross-sectional structure diagram of the separation column in an embodiment of the present application;

[0020] Figure 3 is Figure 2 a schematic diagram of the disassembled state of the separation column in

[0021] Description of reference numerals:

[0022] 1 - Tube body; 11 - First tube body; 111 - First injection port; 112 - Separation sieve; 12 - Second tube body; 121 - Flow passage; 122 - Second injection port; 123 - Output port; 124 - Liquid through hole;

[0023] 2 - Cover body; 21 - Injection port; 22 - First connecting member; 221 - First internal thread; 222 - First external thread; 23 - Second connecting member; 231 - Second internal thread; 232 - Second external thread;

[0024] 3 - Separation chamber;

[0025] 4 - Infrared sensor;

[0026] 5 - Flow controller;

[0027] 6 - Waste liquid collector. Detailed description of the specific embodiments

[0028] Now, various exemplary embodiments of the present application will be described in detail with reference to the accompanying drawings. It should be noted that: Unless otherwise specifically stated, the relative arrangements of components and steps, numerical expressions, and numerical values set forth in these embodiments do not limit the scope of the present application.

[0029] The following description of at least one exemplary embodiment is merely illustrative in nature and in no way serves as a limitation to the present application or its application or use.

[0030] Technologies, methods, and devices known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and devices should be regarded as part of the specification.

[0031] In all the examples shown and discussed herein, any specific value should be construed as merely exemplary and not as a limitation. Therefore, other examples of the exemplary embodiments may have different values.

[0032] It should be noted that like reference numerals and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof in subsequent figures is not necessary.

[0033] According to an embodiment of the present application, a nested peripheral blood leukocyte separation column is provided. The nested peripheral blood leukocyte separation column includes a tube body 1 and a cover body 2, and the cover body 2 is connected to the tube body 1; wherein, the tube body 1 includes a first tube body 11 and a second tube body 12, the first tube body 11 is located inside the second tube body 12, the first tube body 11 has a first injection port 111 and a separation sieve 112, the first injection port 111 and the separation sieve 112 are respectively arranged at opposite ends of the first tube body 11, a circulation passage 121 is arranged inside the second tube body 12, the circulation passage 121 is communicated with the separation sieve 112, the second tube body 12 has a second injection port 122 and an output port 123, and the second injection port 122 and the output port 123 are respectively communicated with opposite ends of the circulation passage 121.

[0034] As Figures 1 to 3 shown, the tube body 1 is a cylindrical tube with an opening at the top and a conical bottom. The cover body 2 is connected to the tube body 1 by means of threads, snap connection or interference fit, etc.

[0035] The tube body 1 includes a first tube body 11 and a second tube body 12. The diameter dimension of the first tube body 11 is smaller than that of the second tube body 12. The length dimension of the first tube body 11 is smaller than the length dimension of the first tube body 11. The first tube body 11 can be placed inside the second tube body 12.

[0036] An opening is provided at the top of the first tube body 11. This opening is the first injection port 111. Samples can be put into the first tube body 11 through the first injection port 111. At the bottom of the first tube body 11, that is, at the conical end of the first tube body 11, a separation sieve 112 is provided. By providing the separation sieve 112, the lysed cell debris is separated from the first tube body 11.

[0037] The pore size of the separation sieve 112 is between that of red blood cell debris and white blood cells. The separation sieve 112 can separate the two and retain white blood cells.

[0038] The mesh pore size of the separation sieve 112 is between 8.5 microns and 10 microns.

[0039] Of course, in the embodiment of the present application, the separation sieve 112 is not limited to the above structure, and those skilled in the art can set it according to actual needs.

[0040] The second tube body 12 has a second injection port 122 and an output port 123. The cleaning liquid can be injected into the second tube body 12 through the second injection port 122, and the used cleaning liquid can be drawn out of the second tube body 12 through the output port 123.

[0041] A flow passage 121 is provided in the second tube body 12. The second injection port 122 and the output port 123 are respectively communicated with the flow passage 121. The flow passage 121 is opened along the inner wall of the second tube body 12. The cleaning liquid is injected into the flow passage 121 through the second injection port 122, and the cleaning liquid flows along the flow passage 121. The flow passage 121 is communicated with the separation sieve 112.

[0042] When the cleaning liquid flows through the bottom of the first tube body 11 through the flow passage 121, the red blood cell fragments separated by the separation sieve 112 fall into the second tube body 12. When the flow passage 121 flows through the bottom of the first tube body 11, the cleaning liquid flushes away the red blood cell fragments at the bottom of the first tube body 11 and discharges the impurities-containing liquid through the output port 123 through the output port 123.

[0043] In the embodiment of the present application, the sample is lysed and screened out through the first tube body, effectively improving the ability to capture peripheral blood white blood cells. The cleaning liquid is transported through the flow passage of the second tube body to drive the lysed red blood cell fragments to output from the second tube body through the cleaning liquid, effectively improving the separation purity of the cells, and the recovery rate also increases. Moreover, through the combined use of the cover body, the first tube body and the second tube body, the operation of the separation column is simple, and the overall cost is relatively low, which is convenient for popularization and application.

[0044] In one example, the cover body 2 can be detachably connected to the first tube body 11 and the second tube body 12 respectively.

[0045] Such as Figure 1 and Figure 2 As shown, the cover body 2 can be connected to the first tube body 11 and the second tube body 12 respectively. By connecting the cover body 2 to the first tube body 11 and the second tube body 12 respectively, the sample contamination in the first tube body 11 can be effectively reduced, and the separation purity of the sample in the first tube body 11 can be guaranteed.

[0046] Of course, in the embodiment of the present application, the first tube body 11 and the second tube body 12 are not limited to the above structures, and those skilled in the art can set them according to actual needs. For example, the cover body 2 is connected to the end of the first injection port 111 of the first tube body 11, the first tube body 11 and the second tube body 12 are detachably connected, and the first tube body 11 and the second tube body 12 can be connected by means of threads, snap connections or interference fits, etc., so that the first tube body 11 is embedded in the second tube body 12.

[0047] In one example, the cover body 2 has an injection port 21, a first connecting member 22, and a second connecting member 23. The first connecting member 22 is located within the second connecting member 23. The first connecting member 22 is connected to the first tube body 11, and the second connecting member 23 is connected to the second tube body 12. The injection port 21 is provided on the first connecting member 22 and is in communication with the first injection port 111.

[0048] As Figures 1 to 3 shown, the cover body 2 is columnar, and the bottom of the cover body 2 can be detachably connected to the first tube body 11 and the second tube body 12 respectively.

[0049] A protrusion is provided at the top of the cover body 2. An injection port 21 is provided along the axial direction of the cover body 2. The injection port 21 of the cover body 2 is a sample injection port. The injection port 21 is in communication with the first injection port 111 of the first tube body 11. Through the injection port 21, samples can be introduced into the first tube body 11 in a state where the cover body 2 is connected to the first tube body 11. By providing the injection port 21, it is not only convenient to inject samples into the first tube body 11, but also the contact between the first tube body 11 and the outside can be reduced, effectively reducing the contamination of the first tube body 11.

[0050] In one example, the cover body 2, the first tube body 11, and the second tube body 12 are all connected by threads. The first connecting member 22 is provided with a first internal thread 221, and the end of the first tube body 11 near the first injection port 111 is provided with a first external thread 222. The first internal thread 221 and the first external thread 222 are matched; the second connecting member 23 is provided with a second internal thread 231, and the end of the second tube body 12 near the second injection port 122 is provided with a second external thread 232. The second internal thread 231 and the second external thread 232 are matched.

[0051] As Figures 1 to 3 shown, both the first tube body 11 and the second tube body 12 are cylindrical tubes with openings at the top. The cover body 2 is columnar, and the bottom of the cover body 2 has an annular connecting portion.

[0052] As Figure 3 shown, the annular connecting portion includes the first connecting member 22. The inner wall of the first connecting member 22 is provided with a first internal thread 221, and the outer wall of the first tube body 11 near the top is provided with a first external thread 222 that matches the first internal thread 221. That is to say, the first connecting member 22 of the cover body 2 can be connected to the top of the first tube body 11, and the injection port 21 on the cover body 2 is in communication with the first injection port 111 of the first tube body 11.

[0053] As Figure 3As shown, the annular connecting part includes a second connecting member 23. The inner wall of the second connecting member 23 is provided with a second internal thread 231, and the outer wall of the second pipe body 12 near the top is provided with a second external thread 232 that matches the second internal thread 231. That is to say, the second connecting member 23 of the cover body 2 can be connected to the top of the second pipe body 12, and in the state where the cover body 2 is connected to the second pipe body 12, both the second injection port 122 and the output port 123 are located below the second connecting member 23.

[0054] In one example, the flow passage 121 has a liquid through-hole 124. The liquid through-hole 124 is provided between the second injection port 122 and the output port 123. The liquid through-hole 124 is located at one end of the second pipe body 12 away from the cover body 2, and the liquid through-hole 124 is arranged corresponding to the separation sieve 112.

[0055] As Figures 1 to 3 shown, the flow passage 121 is opened along the inner wall of the second pipe body 12, and the flow passage 121 flows through one end close to the separation sieve 112. A liquid through-hole 124 is opened at a position corresponding to the separation sieve 112 in the flow passage 121.

[0056] The flow passage 121 is U-shaped along the longitudinal section of the second pipe body 12. The second injection port 122 and the output port 123 are respectively located at opposite ends of the top of the flow passage 121.

[0057] Of course, in the embodiment of the present application, the flow passage 121 is not limited to the above structure, and those skilled in the art can set it according to actual needs.

[0058] When the cleaning liquid enters the flow passage 121 through the second injection port 122, the cleaning liquid flows along the flow passage 121. When the cleaning liquid flows to the liquid through-hole 124, it overflows through the liquid through-hole 124. The red blood cell fragments lysed in the first pipe body 11 are separated from the first pipe body 11 through the separation sieve 112 and fall into the second pipe body 12 through the sieve holes of the separation sieve 112. The cleaning liquid overflows through the liquid through-hole 124 to wash the bottom in the second pipe body 12. That is to say, the red blood cell fragments in the second pipe body 12 are washed by the overflowing cleaning liquid. The cleaning liquid containing red blood cell fragments flows to the output port 123 through the flow passage 121, and the waste liquid in the flow passage 121 is discharged from the second pipe body 12 through the output port 123, so that new cleaning liquid enters the flow passage 121 to continue washing the red blood cell fragments in the second pipe body 12.

[0059] Of course, in the embodiments of the present application, the liquid passage hole 124 is not limited to the above structure, and those skilled in the art can set it according to actual needs. For example, in the state where the cover body 2 is connected to the first pipe body 11 and the second pipe body 12, there is a gap between the first pipe body 11 and the second pipe body 12. The flow passage 121 is arranged along the gap, and the liquid passage hole 124 is arranged in the flow passage 121. The cleaning liquid can flow through the liquid passage hole 124 into the gap, and flow to the separation screen 112 through the tube walls of the first pipe body 11 and the second pipe body 12 to wash the red blood cell fragments falling into the second pipe body 12. The outlet 123 is arranged at the end of the second pipe body 12 close to the separation screen 112, and the waste liquid containing red blood cell fragments after washing is discharged through the outlet 123.

[0060] In one example, the pipe body 1 has a first end and a second end arranged oppositely, and the cover body 2 is respectively connected to the first ends of the first pipe body 11 and the second pipe body 12; in the state where the cover body 2 is respectively connected to the first pipe body 11 and the second pipe body 12, a separation cavity 3 is formed between the second end of the first pipe body 11 and the second end of the second pipe body 12, and the separation cavity 3 is respectively communicated with the separation screen 112 and the liquid passage hole 124.

[0061] As Figures 1 to 3 shown, the pipe body 1 has a first end and a second end. That is to say, the first end of the pipe body 1 is the top end and can be connected to the cover body 2, and the second end of the pipe body 1 is the bottom end, which is conical to facilitate the collection of the waste after lysis.

[0062] External threads are provided at the first ends of both the first pipe body 11 and the second pipe body 12, and the first ends of the first pipe body 11 and the second pipe body 12 are respectively connected to the cover body 2.

[0063] The diameter dimension of the first pipe body 11 is smaller than the diameter dimension of the second pipe body 12. The length dimension of the first pipe body 11 is smaller than the length dimension of the first pipe body 11. The first pipe body 11 can be placed inside the second pipe body 12.

[0064] As Figures 1 to 3 shown, in the state where the cover body 2, the first pipe body 11, and the second pipe body 12 are connected, the outer wall of the first pipe body 11 fits against the inner wall of the second pipe body 12, and there is a gap between the bottom of the first pipe body 11 and the bottom of the second pipe body 12. The bottom of the first pipe body 11 and the bottom of the second pipe body 12 form a separation cavity 3 for storing the lysed red blood cell fragments. The lysed red blood cell fragments fall into the separation cavity 3 through the separation screen 112, and the cleaning liquid overflows through the liquid passage hole 124 and flushes the separation cavity 3, taking away the red blood cell fragments in the separation cavity 3 and discharging them from the second pipe body 12.

[0065] In one example, the nested peripheral blood leukocyte separation column further includes an infrared sensor 4, and the infrared sensor 4 is disposed on the tube body 1. The infrared sensor 4 is used to monitor the liquid level between the separation sieve 112 and the liquid through hole 124.

[0066] As Figure 1 shown, the infrared sensor 4 is used to monitor the water level of the cleaning liquid in the separation chamber 3. By setting the infrared sensor 4, the flow rate of the cleaning liquid can be monitored.

[0067] When the water level of the cleaning liquid in the separation chamber 3 is too high, it is necessary to adjust the flow rate of the cleaning liquid entering the flow passage 121, reduce the flow rate of the cleaning liquid entering the flow passage 121, and avoid the water level of the cleaning liquid being too high, resulting in the cleaning liquid entering the first tube body 11 through the separation sieve 112 and contaminating the sample in the first tube body 11.

[0068] When the water level of the cleaning liquid in the separation chamber 3 is too low, it is necessary to adjust the flow rate of the cleaning liquid entering the flow passage 121, increase the flow rate of the cleaning liquid entering the flow passage 121, and avoid the water level of the cleaning liquid being too low to effectively wash the red blood cell fragments in the separation chamber 3, resulting in too many red blood cell fragments in the separation chamber 3 and affecting the quality of the sample in the first tube body 11.

[0069] Of course, in the embodiments of the present application, monitoring the water level is not limited to the above devices, and those skilled in the art can set it according to actual needs. For example, it can also be a liquid level sensor or the like.

[0070] In one example, the nested peripheral blood leukocyte separation column further includes a flow controller 5, and the flow controller 5 is connected to the second injection port 122. The flow controller 5 is used to control the liquid flow rate of the second injection port 122.

[0071] As Figure 1 shown, the output end of the flow controller 5 is communicated with the second injection port 122, and the input end of the flow controller 5 is connected to the cleaning liquid. By controlling the flow rate of the cleaning liquid entering the flow passage 121 through the flow controller 5, not only can the influence of too large or too small flow rate on the sample quality be avoided, but also the labor intensity can be effectively reduced and the labor cost can be reduced.

[0072] In one example, the nested peripheral blood leukocyte separation column further includes a waste liquid collector 6, and the waste liquid collector 6 is communicated with the output port 123. The waste liquid collector is used to store the waste liquid discharged from the flow passage 121.

[0073] As Figure 1 shown, the waste liquid collector 6 can be a waste liquid bucket or a waste liquid collection device with a suction function.

[0074] The waste liquid collector 6 has an input end. The input end of the waste liquid collector 6 is communicated with the output port 123 of the second pipe body 12. By providing the waste liquid collector 6, not only can the flow rate of the waste liquid discharge flow path 121 be enhanced, but also the flushing intensity of the cleaning liquid on the separation chamber 3 can be effectively improved.

[0075] Although some specific embodiments of the present application have been described in detail by way of examples, those skilled in the art should understand that the above examples are for illustrative purposes only and not for limiting the scope of the present application. Those skilled in the art should understand that the above embodiments can be modified without departing from the scope and spirit of the present application. The scope of the present application is defined by the appended claims.

Claims

1. A nested peripheral blood leukocyte separation column, characterized in that, It includes a tube body (1) and a cover body (2), and the cover body (2) is connected to the tube body (1); Among them, the tube body (1) includes a first tube body (11) and a second tube body (12). The first tube body (11) is located inside the second tube body (12). The first tube body (11) has a first injection port (111) and a separation sieve (112). The first injection port (111) and the separation sieve (112) are respectively arranged at opposite ends of the first tube body (11). A circulation passage (121) is arranged inside the second tube body (12), and the circulation passage (121) is communicated with the separation sieve (112). The second tube body (12) has a second injection port (122) and an output port (123), and the second injection port (122) and the output port (123) are respectively communicated with opposite ends of the circulation passage (121).

2. The nested peripheral blood leukocyte separation column according to claim 1, wherein The cover body (2) can be detachably connected to the first tube body (11) and the second tube body (12) respectively.

3. The nested peripheral blood leukocyte separation column according to claim 2, wherein, The cover body (2) has an injection port (21), a first connecting member (22) and a second connecting member (23). The first connecting member (22) is located inside the second connecting member (23). The first connecting member (22) is connected to the first tube body (11), and the second connecting member (23) is connected to the second tube body (12). The injection port (21) is arranged on the first connecting member (22), and the injection port (21) is communicated with the first injection port (111).

4. The nested peripheral blood leukocyte separation column according to claim 3, characterized in that, The cover body (2), the first tube body (11) and the second tube body (12) are all connected by threads. The first connecting member (22) is provided with a first internal thread (221), and the end of the first tube body (11) near the first injection port (111) is provided with a first external thread (222), and the first internal thread (221) and the first external thread (222) are matched; the second connecting member (23) is provided with a second internal thread (231), and the end of the second tube body (12) near the second injection port (122) is provided with a second external thread (232), and the second internal thread (231) and the second external thread (232) are matched.

5. The nested peripheral blood leukocyte separation column according to claim 2, wherein The circulation passage (121) has a liquid through hole (124). The liquid through hole (124) is arranged between the second injection port (122) and the output port (123). The liquid through hole (124) is located at one end of the second tube body (12) away from the cover body (2), and the liquid through hole (124) is arranged corresponding to the separation sieve (112).

6. The nested peripheral blood leukocyte separation column according to claim 5, characterized in that The tube body (1) has a first end and a second end arranged oppositely, and the cover body (2) is respectively connected to the first ends of the first tube body (11) and the second tube body (12); In a state where the cover body (2) is connected to the first pipe body (11) and the second pipe body (12), a separation cavity (3) is formed between the second end of the first pipe body (11) and the second end of the second pipe body (12), and the separation cavity (3) is respectively communicated with the separation sieve (112) and the liquid through hole (124).

7. The nested peripheral blood leukocyte separation column according to claim 5, wherein It further includes an infrared sensor (4), and the infrared sensor (4) is arranged on the pipe body (1), and the infrared sensor (4) is used for monitoring the liquid level between the separation sieve (112) and the liquid through hole (124).

8. The nested peripheral blood leukocyte separation column according to claim 1, wherein, It further includes a flow controller (5), and the flow controller (5) is connected to the second injection port (122), and the flow controller (5) is used for controlling the liquid flow rate of the second injection port (122).

9. The nested peripheral blood leukocyte separation column according to claim 1, wherein It further includes a waste liquid collector (6), and the waste liquid collector (6) is communicated with the output port (123), and the waste liquid collector is used for storing the waste liquid discharged from the flow passage (121).

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