Rapid detection reagent card for Rh blood type typing

Abstract

The utility model discloses an Rh blood typing rapid detection reagent card which comprises a reagent card shell, five detection strips arranged at equal intervals are arranged in the reagent card shell, and detection holes used for observing changes of a detection line and a quality control line are formed in the positions, corresponding to the detection strips, of the reagent card shell. A sample adding hole for dropwise adding a sample to be detected is formed in the position, corresponding to the detection strip, of the reagent card shell, and a detection indication line and a control indication line are further arranged in the position, corresponding to the detection strip, of the reagent card shell. The rapid detection reagent card for Rh blood type typing is designed by taking a colloidal gold immunochromatography technology as a main principle, is specially used for detecting Rh blood type antigens C, c, D, E and e, is convenient to carry, simple and convenient to operate, rapid in detection and easy in interpretation, and is particularly suitable for operators in primary hospitals or remote areas, so that the rapid detection reagent card has good practicability.

Description

Technical Field

[0001] The utility model relates to the technical field of blood type detection, in particular to an Rh blood type rapid detection reagent card. Background Art

[0002] The Rh blood group system is the most complex of the human red blood cell types, and its importance in clinical blood transfusion is second only to the ABO blood group system. The RH blood group system primarily consists of five antigens: C, c, D, E, and e. Currently, only the D antigen is a mandatory test item before clinical blood transfusion. Most hospitals, especially those at the grassroots level, lack the conditions to test and match the other antigens of the Rh blood group system during blood transfusions, resulting in patients receiving foreign antigens and developing unexpected antibodies. In recent years, cases of unexpected antibody production due to transfusions of blood with mismatched Rh antigens have become common, posing a significant threat to transfusion safety. Currently, the main method for Rh typing is the microcolumn gel method, which provides relatively accurate results but often requires appropriate instruments and equipment, is cumbersome to operate, and requires professionally trained technicians to interpret the results, placing high demands on personnel. Utility Model Content

[0003] The purpose of the utility model is to provide an Rh blood typing rapid detection reagent card. The Rh blood typing rapid detection reagent card is designed based on the colloidal gold immunochromatographic technology as the main principle. It is specially used to detect Rh blood group antigens C, c, D, E, and e. The design is easy to carry, simple to operate, and the detection is fast and easy to interpret. It is especially suitable for operators in grassroots hospitals or remote areas, and therefore has good practicality.

[0004] In order to achieve the above-mentioned purpose, the main technical solutions adopted by this utility model include:

[0005] A rapid Rh blood typing test reagent card, comprising:

[0006] A reagent card housing is provided with five test strips arranged at equal distances inside the reagent card housing, a detection hole for observing changes in the test line and the quality control line is provided on the reagent card housing at positions corresponding to the test strips, a sample addition hole for adding the sample to be tested is provided on the reagent card housing at positions corresponding to the test strips, and a detection indicator line and a control indicator line are also provided on the reagent card housing at positions corresponding to the test strips.

[0007] The above-mentioned Rh blood typing rapid detection reagent card, wherein the detection strip is composed of a PVC base plate, a reaction membrane, a gold conjugate pad, a water absorbent pad and a sample pad.

[0008] The above-mentioned Rh blood typing rapid detection reagent card, wherein the gold conjugate pad is respectively coated with colloidal gold-labeled mouse anti-human D antigen monoclonal antibody, mouse anti-human C antigen monoclonal antibody, mouse anti-human c antigen monoclonal antibody, mouse anti-human E antigen monoclonal antibody, and mouse anti-human e antigen monoclonal antibody.

[0009] The above-mentioned Rh blood typing rapid detection reagent card, wherein the reaction membrane is respectively provided with a D antigen detection line coated with mouse anti-human D antigen monoclonal antibody, a C antigen detection line coated with mouse anti-human C antigen monoclonal antibody, a C antigen detection line coated with mouse anti-human C antigen monoclonal antibody, an E antigen detection line coated with mouse anti-human E antigen monoclonal antibody, and an E antigen detection line coated with mouse anti-human E antigen monoclonal antibody.

[0010] The above-mentioned Rh blood typing rapid detection reagent card, wherein the reaction membrane is provided with a quality control line coated with sheep anti-mouse IgG polyclonal antibody.

[0011] The above-mentioned Rh blood typing rapid detection reagent card, wherein the reagent card shell is in the shape of a rectangular box.

[0012] In the above-mentioned Rh blood typing rapid detection reagent card, the plurality of sample addition holes correspond one to one with the plurality of detection strips.

[0013] The utility model has at least the following beneficial effects:

[0014] The utility model provides an Rh blood typing rapid detection reagent card, which is designed based on the colloidal gold immunochromatographic technology as the main principle. It is specially used to detect Rh blood group antigens C, c, D, E, and e. The design is easy to carry, simple to operate, and the detection is fast and easy to interpret. It is suitable for hospitals at all levels, especially the blood transfusion departments of grassroots hospitals. It can quickly detect the patient's Rh blood type major antigens without professional equipment, thereby achieving accurate cross-matching and reducing the production of unexpected Rh system antibodies. Therefore, it has good practicality.

[0015] In addition, the detection indicator line and the control indicator line are arranged on the reagent card housing and at the position corresponding to the detection strip. Under the synergistic effect of the detection indicator line and the control indicator line, the reliability and accuracy of the test result can be improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0017] Figure 1 This is a schematic structural diagram of the reagent card housing in the Rh blood typing rapid detection reagent card of the present invention;

[0018] Figure 2 This is a schematic diagram of the structure of the Rh blood typing rapid detection reagent card of the present utility model;

[0019] Figure 3 This is a partial schematic diagram of the internal detection strip of the Rh blood typing rapid detection reagent card of the present invention.

[0020] Description of Figure Numbers:

[0021] 1. Reagent card housing;

[0022] 101, test strip; 102, sample well; 103, test indicator line; 104, control indicator line;

[0023] 1041. PVC base plate; 1042. Reaction membrane; 1043. Gold conjugate pad; 1044. Water-absorbing pad; 1045. Sample pad. DETAILED DESCRIPTION

[0024] The following will describe the implementation methods of the present application in detail with reference to the accompanying drawings and examples, so that the implementation process of how the present application applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0025] In the description of the embodiments of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present invention.

[0026] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly stipulated and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, an integral connection, or a detachable connection; it can be the internal connection of two components; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0027] Please refer to Figures 1 to 3As shown, this embodiment provides an Rh blood typing rapid detection reagent card, comprising: a reagent card housing 1, wherein five test strips 101 arranged at equal distances are provided inside the reagent card housing 1, a detection hole for observing changes in the test line and the quality control line is provided on the reagent card housing 1 and at positions corresponding to the test strips 101, a sample addition hole 102 for dripping a sample to be tested is provided on the reagent card housing 1 and at positions corresponding to the test strips 101, and a detection indicator line 103 and a control indicator line 104 are also provided on the reagent card housing 1 and at positions corresponding to the test strips 101;

[0028] The detection indicator line 103 and the control indicator line 104 are arranged on the reagent card housing 1 and at positions corresponding to the detection strip 101. Under the synergistic effect of the detection indicator line 103 and the control indicator line 104, the reliability and accuracy of the detection result can be improved.

[0029] Please refer to Figures 1 to 3 As shown, the test strip 101 is composed of a PVC base plate 1041 , a reaction membrane 1042 , a gold conjugate pad 1043 , a water absorbent pad 1044 and a sample pad 1045 .

[0030] Please refer to Figures 1 to 3 As shown, the gold conjugate pad 1043 is coated with colloidal gold-labeled mouse anti-human D antigen monoclonal antibody, mouse anti-human C antigen monoclonal antibody, mouse anti-human c antigen monoclonal antibody, mouse anti-human E antigen monoclonal antibody, and mouse anti-human e antigen monoclonal antibody.

[0031] Please refer to Figures 1 to 3 As shown, the reaction membrane 1042 is respectively provided with a D antigen detection line coated with mouse anti-human D antigen monoclonal antibody, a C antigen detection line coated with mouse anti-human C antigen monoclonal antibody, a C antigen detection line coated with mouse anti-human C antigen monoclonal antibody, an E antigen detection line coated with mouse anti-human E antigen monoclonal antibody, and an e antigen detection line coated with mouse anti-human e antigen monoclonal antibody.

[0032] Please refer to Figures 1 to 3 As shown, a quality control line coated with goat anti-mouse IgG polyclonal antibody is provided on the reaction membrane 1042 .

[0033] Please refer to Figures 1 to 3 As shown, the reagent card housing 1 is in the shape of a rectangular box.

[0034] Please refer to Figures 1 to 3 As shown, a plurality of sample addition wells 102 correspond one to one with a plurality of detection strips 101;

[0035] By adopting the above technical solution, an Rh blood typing rapid detection reagent card was designed based on the colloidal gold immunochromatographic technology as the main principle. It is specifically used to detect Rh blood type antigens C, c, D, E, and e. The design is easy to carry, simple to operate, and the detection is fast and easy to interpret. It is suitable for hospitals at all levels, especially the blood transfusion department of grassroots hospitals. It can quickly detect the patient's Rh blood type major antigens without professional equipment, thereby achieving accurate cross-matching and reducing the production of unexpected antibodies in the RH system. Therefore, it has good practicality.

[0036] The working principle of the present invention is as follows: place the reagent card flat on the table, add 15-30 μL of whole blood to the sample addition hole 102, and then add about 150 μL of sample diluent, and read the results in 3 to 5 minutes. The appearance of red strips at both the test indicator line 103 and the control indicator line 104 indicates that the sample contains the corresponding antigen. The appearance of a red strip only at the quality control line indicates that the sample does not contain the corresponding antigen. The absence of a red strip at both positions indicates that the test is invalid.

[0037] The above description shows and describes several preferred embodiments of the present invention. However, as mentioned above, it should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Instead, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the present invention through the above teachings or the technology or knowledge in the relevant field. Modifications and variations made by those skilled in the art without departing from the spirit and scope of the present invention are intended to be protected by the claims appended hereto.

Claims

1. A rapid detection reagent card for Rh blood typing, characterized in that: include: A reagent card housing (1), wherein five test strips (101) arranged at equal distances are provided inside the reagent card housing (1); a detection hole for observing changes in a test line and a quality control line is provided on the reagent card housing (1) and at a position corresponding to the test strips (101); a sample addition hole (102) for dripping a sample to be tested is provided on the reagent card housing (1) and at a position corresponding to the test strips (101); and a detection indicator line (103) and a control indicator line (104) are also provided on the reagent card housing (1) and at a position corresponding to the test strips (101); The detection strip (101) is composed of a PVC base plate (1041), a reaction membrane (1042), a gold conjugate pad (1043), a water-absorbing pad (1044) and a sample pad (1045); the gold conjugate pad (1043) is coated with colloidal gold-labeled mouse anti-human D antigen monoclonal antibody, mouse anti-human C antigen monoclonal antibody, mouse anti-human C antigen monoclonal antibody, mouse anti-human E antigen monoclonal antibody and mouse anti-human E antigen monoclonal antibody; the reaction membrane (1042) is provided with a D antigen detection line coated with mouse anti-human D antigen monoclonal antibody, a C antigen detection line coated with mouse anti-human C antigen monoclonal antibody, a C antigen detection line coated with mouse anti-human C antigen monoclonal antibody, an E antigen detection line coated with mouse anti-human E antigen monoclonal antibody and an E antigen detection line coated with mouse anti-human E antigen monoclonal antibody.

2. The Rh blood typing rapid detection reagent card according to claim 1, characterized in that: The reaction membrane (1042) is provided with a quality control line coated with goat anti-mouse IgG polyclonal antibody.

3. The Rh blood typing rapid detection reagent card according to claim 2, characterized in that: The reagent card housing (1) is in the shape of a rectangular box.

4. The Rh blood typing rapid detection reagent card according to claim 3, characterized in that: The plurality of sample addition holes (102) correspond one to one with the plurality of detection strips (101).

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