Mouse saliva collecting device
Through the design of the separation screen and centrifugal tube, the saliva suction piece and mouse saliva are separated by centrifugal force, solving the problems of low efficiency of saliva collection and difficulty in quantitative measurement in mice, achieving efficient and simplified saliva collection and measurement.
Patent Information
- Application Number
- CN202422165672.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-04
- Publication Date
- 2025-08-08
- Estimated Expiration
- 2034-09-04
AI Technical Summary
The existing mice saliva collection methods are inefficient, have numerous processes, and have large safety risks and errors, making it difficult to efficiently collect and quantitatively measure the saliva flow rate.
The structure of the separation screen and centrifugal tube is adopted, and the centrifugal tube is removably connected to the centrifugal tube through the cover. The saliva suction piece and mouse saliva are separated by centrifugal force, combined with the guide bar and convex column design, improve the saliva separation efficiency and quantitative measurement accuracy.
The mouse saliva collection process is simplified, the collection efficiency is improved, manpower consumption is reduced, the experimental preparation time is shortened, and the error is reduced.
Smart Images
Figure CN223196088U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of experimental tools, and more particularly to a mouse saliva collection device. Background Art
[0002] Some studies require collecting mouse saliva for component analysis or measuring its salivary flow rate (the amount of saliva produced per unit time by a mouse of a given body weight). However, the salivary flow rate of mice is very low (after stimulation with pilocarpine, the salivary flow rate is approximately 40 μl / min / 100 g; for example, a typical 25 g mouse produces approximately 100 μl of saliva in 10 minutes), making the collection and quantitative measurement of mouse saliva difficult.
[0003] Common methods for obtaining mouse saliva and measuring its salivary flow rate include: directly aspirating from the mouse's mouth with a pipette for a period of time; placing the mouse upside down with a centrifuge tube placed at the floor of the mouth to collect the naturally flowing saliva for a period of time and quantitatively measuring it; placing a capillary tube at the floor of the mouse's mouth, allowing the saliva to flow down the capillary tube into the small tube for a period of time and then measuring its weight; placing an absorbent material such as a cotton ball, cotton pad, gauze strip, sponge, or capillary tube in the mouse's mouth for a period of time and removing it after a period of time, and then calculating the weight of the mouse saliva during that period by the difference in weight before and after (wet weight method); placing the material soaked in mouse saliva into a small centrifuge tube with a hole drilled in the bottom, inserting it into a larger centrifuge tube, centrifuging it, and then aspirating the saliva at the bottom of the large centrifuge tube for quantitative measurement. These methods take 5 to 30 minutes to collect saliva and generally require the injection of pilocarpine to stimulate saliva secretion, which requires anesthesia of the mouse.
[0004] In the above schemes, there are certain operational difficulties in directly sucking out saliva with a pipette and collecting it by inverting the mouse, which consumes a lot of manpower and may result in a small amount of saliva collected; the method of placing an absorbent material in the mouse's mouth to suck out saliva has the risk of causing the mouse to suffocate, and can only measure the mass of saliva and indirectly infer the volume of saliva; the method of sucking out saliva with an absorbent material and combining it with the method of nesting and drilling large and small centrifuge tubes for centrifugation, due to the lack of relevant finished products on the market, requires manual processing such as cutting off the cap of the small centrifuge tube and perforating with a needle before use. The process is relatively complicated, and the large and small centrifuge tubes may not be compatible. After nesting, the cap of the large centrifuge tube cannot be covered. Before centrifugation, the tube mouth needs to be covered with plastic wrap to prevent saliva evaporation, and the hole at the bottom of the small centrifuge tube may be clogged by debris that was not cleaned up during manual processing, making it difficult for saliva to enter the large centrifuge tube from the small centrifuge tube. A mouse saliva collection device is urgently needed to improve the collection efficiency and simplify the process. Utility Model Content
[0005] The utility model overcomes the shortcomings of the existing mouse saliva collection, which requires self-prepared tools, has low collection efficiency and many processes, and provides a mouse saliva collection device, which improves the collection efficiency and simplifies the process.
[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions:
[0007] A mouse saliva collection device comprises a cover, a separation sieve, a centrifuge tube, and a saliva suction piece. The bottom or side wall of the separation sieve is provided with an opening for passing liquid. The top of the separation sieve is open and has a placement cavity for placing the saliva suction piece. The separation sieve is detachably mounted in the centrifuge tube, with a gap provided between the bottom and the bottom wall of the centrifuge tube. The cover is detachably connected to the tube mouth of the centrifuge tube.
[0008] A centrifuge tube is loaded with a separation sieve, which is then filled with a saliva suction piece and sealed with a lid. The centrifuge tube is then placed in a centrifuge, allowing the suction piece to separate from the absorbed mouse saliva, allowing the mouse saliva to enter the tube through the openings in the separation sieve. Because there is a gap between the separation sieve and the bottom wall of the centrifuge tube, the level of the saliva after separation is lower than the separation sieve, preventing the saliva from remixing with the suction piece. After separation, the separation sieve can be removed from the centrifuge tube, and the mouse saliva in the centrifuge tube can be aspirated using a pipette. The suction piece is sized to fit the mouse's oral cavity. During use, the suction piece is first placed in the mouse's oral cavity, then removed after a predetermined time and placed in the separation sieve. After sealing with the lid, the centrifuge tube is placed in a centrifuge for separation. After separation, the separation sieve is removed, and the saliva in the centrifuge tube is aspirated using a pipette, completing the collection of the mouse saliva.
[0009] Preferably, the top side wall of the separation screen is provided with a positioning flange, and the positioning flange is mounted on the side wall of the centrifuge tube, so that the separation screen and the centrifuge tube are detachably connected in this way.
[0010] Preferably, the side wall of the separation screen is provided with a number of equally spaced hollows, and the bottom of the separation screen is hemispherical and provided with openings. The large area of hollows balances the air pressure of the separation screen and the tube body, and the area for leakage is large, which is conducive to the leakage of saliva and the removal of the separation screen.
[0011] Preferably, the cover body is threadedly connected to the inner wall of the centrifuge tube to achieve a detachable connection between the cover body and the centrifuge tube.
[0012] Preferably, the bottom wall of the cover body has a sealing gasket, and when the cover body is connected to the centrifuge tube, the sealing gasket fits on the positioning flange to improve the sealing of the collection device.
[0013] Preferably, a convex column is provided on the cover body, the convex column abuts against the saliva suction piece, and the convex column compresses the saliva suction piece to improve the saliva separation efficiency.
[0014] Preferably, the saliva suction piece is a cylinder with a ring-shaped cross section, and the through hole in the middle of the saliva suction piece reduces the risk of mouse suffocation during the process of sucking mouse saliva, and is convenient for clamping with tweezers.
[0015] Preferably, the lower portion of the centrifuge tube is conical and has scales, and the structure facilitates the collection device to count the amount of saliva.
[0016] Preferably, the inner wall surface of the centrifuge tube is provided with a hydrophobic coating to reduce saliva adhesion residue.
[0017] Preferably, the side walls of the separation screen are provided with guide strips for guiding the twisting of the saliva suction piece, the guide strips being arranged in a spiral shape, and the spiral directions of the guide strips on the upper portion of the separation screen and the guide strips on the lower portion of the separation screen are opposite to each other. The structure utilizes the centrifugal force directed toward the bottom of the centrifuge tube to drive the saliva suction piece to twist along the guide strips during its downward movement, thereby squeezing out saliva in a twisting manner.
[0018] Preferably, the boss is threadedly connected to the cover, with the threads on the boss running in the opposite direction to the threads on the guide strips on the upper portion of the separation screen. The boss has a greater density than the saliva suction member. When the saliva suction member is twisted under the action of the guide strips, friction drives the boss to rotate, thereby releasing the connection with the top cover and freeing the boss. Furthermore, due to the greater density of the boss, centrifugal force exerts continuous pressure on the saliva suction member, thereby improving the efficiency of saliva extraction.
[0019] Preferably, the shape of the protrusion is adapted to the caliber of the separation screen, and the protrusion is limited by the side wall of the separation screen to prevent the protrusion from tilting and causing uneven pressure on the surface of the saliva suction piece, which may cause damage to the saliva suction piece.
[0020] Compared with the prior art, the beneficial effects of the present invention are:
[0021] The structure of separation sieve and centrifuge tube is used to separate the saliva suction piece and mouse saliva, which is convenient for collecting mouse saliva and measuring its volume and quality, saving manpower, shortening experimental preparation time and reducing errors. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is an exploded view of the utility model;
[0023] Figure 2 It is a cross-sectional view of the separation tube of the present utility model;
[0024] Figure 3 It is a schematic diagram of the cover body of the present utility model;
[0025] Figure 4 This is the separation screen of Example 1 of the present utility model;
[0026] Figure 5 This is the separation screen of Example 2 of the present utility model;
[0027] In the picture:
[0028] Cover body 1, separation screen 2, centrifuge tube 3, saliva suction piece 4, positioning flange 5, hollow 6, opening 7, sealing gasket 8, protruding column 9, scale 10, guide strip 11. DETAILED DESCRIPTION
[0029] The present disclosure will be further described below with reference to the accompanying drawings and embodiments.
[0030] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present application belongs.
[0031] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.
[0032] In the present disclosure, terms such as "upper", "lower", "left", "right", "front", "back", "vertical", "horizontal", "side", "bottom", etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings. They are merely relational words determined for the convenience of describing the structural relationships of the various parts or elements of the present disclosure, and do not specifically refer to any part or element in the present disclosure, and should not be understood as limitations on the present disclosure.
[0033] In this disclosure, terms such as "fixed connection," "connected," and "connection" should be interpreted broadly to mean a fixed connection, an integral connection, or a detachable connection; a direct connection or an indirect connection through an intermediary. Relevant researchers or technicians in this field may determine the specific meaning of these terms in this disclosure based on specific circumstances, and they should not be construed as limiting this disclosure.
[0034] Example 1:
[0035] A mouse saliva collection device, Figure 1 As shown, it includes a cover 1, a separation sieve 2, a centrifuge tube 3 and a saliva suction piece 4. The bottom or side wall of the separation sieve 2 is provided with an opening 7 for passing liquid. The top of the separation sieve 2 is open and has a placement cavity for placing the saliva suction piece 4. The separation sieve 2 is detachably installed in the centrifuge tube 3 and a gap is provided between the bottom and the bottom wall of the centrifuge tube 3. The cover 1 is detachably connected to the tube mouth of the centrifuge tube 3.
[0036] Ginseng Figure 3As shown, the lid 1 is threadedly connected to the inner wall of the centrifuge tube 3, achieving a removable connection between the lid 1 and the centrifuge tube 3. The outer surface of the lid 1 has friction grooves to enhance friction. The bottom wall of the lid 1 has a sealing gasket 8. When the lid 1 is connected to the centrifuge tube 3, the sealing gasket 8 fits against the positioning flange 5 to enhance the seal of the collection device. The lid 1 is also provided with a boss 9, which abuts the saliva suction member 4 and compresses the saliva suction member 4, thereby improving the efficiency of saliva separation.
[0037] Ginseng Figure 4 As shown, the surface of the separation sieve 2 is coated with a hydrophobic coating, and its size matches the tube body. The top side wall of the separation sieve 2 is provided with a positioning flange 5, which is mounted on the side wall of the centrifuge tube 3. The above method realizes the detachable connection between the separation sieve 2 and the centrifuge tube 3. The side wall of the separation sieve 2 is provided with a plurality of equally spaced hollows 6. The bottom of the separation sieve 2 is hemispherical and has an opening 7. The large area of the hollows 6 balances the air pressure of the separation sieve 2 and the tube body, and the area that can be leaked is large, which is conducive to the leakage of saliva and the removal of the separation sieve 2.
[0038] The saliva suction member 4 is a cylindrical structure with a circular cross-section. A through hole in the center of the saliva suction member 4 reduces the risk of suffocation during saliva extraction and facilitates gripping with tweezers. The ends of the saliva suction member 4 are chamfered. The saliva suction member 4 is sized to fit within the mouse's oral cavity. In some embodiments, it is 18 mm long and 5 mm wide.
[0039] Ginseng Figure 1 、 2 As shown, the lower portion of the centrifuge tube 3 is tapered and has scale marks 10, which facilitates the collection device to count the amount of saliva. The inner surface of the centrifuge tube 3 is provided with a hydrophobic coating to reduce saliva adhesion. The upper half of the outer surface of the centrifuge tube 3 features a frosted area for convenient marking with a marker. The specifications of the centrifuge tube 3 are compatible with most centrifuges.
[0040] A centrifuge tube 3 is loaded with a separation sieve 2, which is then filled with a saliva suction piece 4 and sealed with a cover 1. The centrifuge tube 3 is then placed in a centrifuge, allowing the saliva suction piece 4 to separate from the absorbed mouse saliva, allowing the mouse saliva to enter the centrifuge body through the opening 7 of the separation sieve 2. Because there is a gap between the separation sieve 2 and the bottom wall of the centrifuge tube 3, the saliva level after separation is lower than the separation sieve 2, preventing the saliva from remixing with the saliva suction piece 4. After separation, the separation sieve 2 can be removed from the centrifuge tube 3 to aspirate the mouse saliva in the centrifuge tube 3 using a pipette. The saliva suction piece 4 is sized to fit the oral cavity of a mouse. During use, the saliva suction piece 4 is first placed in the mouse's oral cavity, then removed after a predetermined time and placed in the separation sieve 2. After sealing the centrifuge tube 3 with the cover 1, the centrifuge tube 3 is placed in the centrifuge for separation. After separation, the separation sieve 2 is removed, and the saliva in the centrifuge tube 3 is aspirated using a pipette to complete the collection of the mouse saliva.
[0041] The usage process of this embodiment is as follows:
[0042] Weigh and record the weight of the centrifuge tube 3 containing the saliva suction piece 4.
[0043] 1. Anesthetize the mouse and inject pilocarpine. Place the mouse in a lateral position (to avoid suffocation due to excessive saliva) and wait for the pilocarpine to stimulate saliva secretion to take effect (about 2-3 minutes).
[0044] 2. Make the mouse open its mouth, use tweezers to hold the saliva suction piece 4 and put it into the mouse's mouth, so that it fills the mouse's oral cavity, and start timing.
[0045] 3. Wait for a while (about 15 minutes, the time length can be set according to the specific situation), stop the timer, remove the saliva suction piece 4, put it into the colander in the centrifuge tube 3, tighten the lid 1, weigh and record the total weight. The difference between the total weight and the total weight of the centrifuge tube 3 before saliva suction is the mass of saliva.
[0046] 4. Centrifuge at 8000g for 3 minutes.
[0047] 5. Use tweezers to remove the saliva suction device 4 and separation sieve 2. Use a pipette (set to a larger capacity than the estimated saliva volume, such as 100-150 μl) to aspirate all the saliva from the bottom of the centrifuge tube 3. Gradually reduce the pipette capacity until the saliva in the pipette reaches the pipette tip. The pipette capacity at this point is the saliva volume. If the saliva volume is too small and viscous, dilute it with a known amount of saliva preservative solution or buffer. Subtract the volume of the diluent from the pipette capacity displayed to calculate the saliva volume as described above.
[0048] Example 2:
[0049] On the basis of Example 1, the following further features are included:
[0050] Ginseng Figure 5 As shown, the sidewall of the separation sieve 2 is provided with guide strips 11 for guiding the rotation of the saliva suction member 4. The guide strips 11 are arranged in a spiral pattern, with the spiral directions of the guide strips 11 on the upper and lower portions of the separation sieve 2 opposite to each other. This structure utilizes centrifugal force directed toward the bottom of the centrifuge tube 3 to drive the saliva suction member 4 to rotate along the guide strips 11 during its downward movement, thereby squeezing out saliva. A boss 9 is threadedly connected to the cover 1. The threads on the boss 9 are in the opposite direction to those of the guide strips 11 on the upper portion of the separation sieve 2. The boss 9 has a greater density than the saliva suction member 4. When the saliva suction member 4 is twisted by the guide strips 11, friction drives the boss 9 to rotate, thereby releasing the connection with the top cover and freeing the boss 9. Furthermore, due to its higher density, the boss 9 continuously exerts pressure on the saliva suction member 4 under the centrifugal force, thereby improving the efficiency of saliva extraction. The shape of the protrusion 9 is adapted to the separation screen 2 and the side wall of the separation screen 2 limits the protrusion 9 to avoid uneven pressure on the surface of the saliva suction piece 4 caused by the tilt of the protrusion 9 and resulting in damage to the saliva suction piece 4.
[0051] The outer wall of the saliva suction member 4 has a clearance fit with the separation screen 2. Under the centrifugal force of the centrifuge, the saliva suction member 4 generates a downward force, moving downward and deforming. During this movement, it is guided by the guide bar, and the upper and lower parts rotate in opposite directions, thus more efficiently extracting the mouse's saliva. During this process, the top of the saliva suction member 4 abuts against the boss 9. As the upper part of the saliva suction member 4 rotates, the saliva suction member 4 provides friction to the boss 9, driving the boss 9 to rotate synchronously. During this rotation, the boss 9 loosens the thread on the top cover, eventually separating from the top cover and being subjected to the centrifugal force, pressing the saliva suction member 4 downward, further compressing the saliva suction member 4 and deforming it, squeezing out the saliva.
[0052] The above structure can separate saliva more efficiently and reduce the working time of the centrifuge. The operation of the above mechanism does not depend on the operation, but can be achieved only by structural improvements.
[0053] The above-described embodiments are only preferred solutions of the present invention and do not limit the present invention in any form. Other variations and modifications are possible without exceeding the technical solutions described in the claims.
Claims
1. A mouse saliva collection device, characterized in that: The device comprises a cover, a separation sieve, a centrifuge tube and a saliva suction piece. The bottom or side wall of the separation sieve is provided with an opening for passing liquid. The top of the separation sieve is open and has a placement cavity for placing the saliva suction piece. The separation sieve can be detachably installed in the centrifuge tube with a gap between the bottom and the bottom wall of the centrifuge tube. The cover is detachably connected to the nozzle of the centrifuge tube.
2. A mouse saliva collection device according to claim 1, characterized in that: The top side wall of the separation screen is provided with a positioning flange, and the positioning flange is mounted on the side wall of the centrifuge tube.
3. A mouse saliva collection device according to claim 2, characterized in that: The side wall of the separation screen is provided with a plurality of equally spaced hollows, and the bottom of the separation screen is hemispherical and provided with openings.
4. The mouse saliva collection device according to claim 1, wherein: The cover body is threadedly connected to the inner wall of the centrifuge tube.
5. The mouse saliva collection device according to claim 1, wherein: The bottom wall of the cover body has a sealing gasket.
6. The mouse saliva collection device according to claim 1, wherein: The cover body is provided with a convex column which abuts against the saliva suction piece.
7. The mouse saliva collection device according to claim 1, wherein: The lower part of the centrifuge tube is conical and has scales.
8. The mouse saliva collection device according to claim 7, wherein: The inner wall surface of the centrifuge tube is provided with a hydrophobic coating.
9. The mouse saliva collection device according to claim 1, wherein: The side wall of the separation screen is provided with a guide bar for guiding the rotation of the saliva suction piece. The guide bar is arranged in a spiral shape, and the spiral direction of the guide bar on the upper part of the separation screen is opposite to that of the guide bar on the lower part of the separation screen.
10. The mouse saliva collection device according to claim 9, characterized in that: The boss is threadedly connected to the cover body, the thread direction of the boss is opposite to the thread direction of the guide strip on the upper part of the separation screen, and the density of the boss is greater than that of the saliva suction piece.