Disposable support for micro-CT scanning of in-vitro limb bones of mouse
By designing a scaffold for microCT scanning of ex vivo limb bones in mice, the mouse bones are fixed using internal cavity plugs and soft pads, the bone destruction and artifact problems caused by tape fixation are solved, and clear scanning images and efficient scanning process are achieved.
Patent Information
- Application Number
- CN202422237300.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-12
- Publication Date
- 2025-08-08
- Estimated Expiration
- 2034-09-12
AI Technical Summary
In the prior art, when using tape to fix the ex vivo bones of mice, it is easy to cause bone structure damage and artifacts, affecting the accuracy of the scanning image.
A bracket including a main cylinder, a cylinder cover and a limit fixation structure is designed to limit the mouse bones through internal cavity plugs and soft pads to avoid offsets and keep the bone structure intact.
It realizes stable fixation of bones during scanning, ensures clear and blur-free images, and supports multiple bones to scan simultaneously, improving scanning efficiency and accuracy.
Smart Images
Figure CN223205405U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of medical auxiliary products, in particular to a disposable bracket used for micro CT scanning of isolated limb bones of mice. Background Art
[0002] Micro-CT (Micro Computed Tomography), also known as micro-CT or micro-CT, uses a microfocus X-ray tube to scan and image samples. With a resolution of up to several microns, micro-CT can clearly reveal the finest internal structures of a sample. Micro-CT imaging principles are similar to those of conventional CT, but with higher spatial resolution, making it particularly suitable for detailed analysis of in vitro specimens from small animals.
[0003] Scanning isolated mouse limb bones using Micro-CT is a high-precision, non-destructive imaging method widely used in the biomedical field. Within this field, Micro-CT has been widely used in bone disease research and drug efficacy evaluation. For example, scanning and analyzing isolated mouse femurs using Micro-CT can assess the efficacy of osteoporosis drugs, while scanning the tibia and humerus can help study fracture healing processes and material degradation in vivo. When removing mouse bones, the mouse is first placed in an anesthesia chamber for anesthesia, and then the bones are stripped after anesthesia; when scanning the mouse bones removed from the body, the bone surface must first be cleaned and the attached soft tissue removed, and then the bones are placed on the scanning bed of the Micro-CT device for scanning, and the scanned image is displayed on the display screen; however, the mouse bones removed from the body are small and light, and are easily displaced due to mechanical vibration during the scanning process, resulting in blurred scanned images and inability to read useful information. Therefore, the mouse bones need to be fixed before scanning; the existing method of fixing mouse bones is to use tape to fix the bones to the scanning bed, but this affects the natural shape of the bones and introduces artifacts. In addition, the use of tape to fix the bones will cause direct contact between the bones and the hard surface of the scanning bed, causing damage to the bone structure, thereby affecting the accurate judgment and analysis of the bone structure.
[0004] Therefore, how to design a disposable bracket for micro-CT scanning of mouse ex vivo limb bones that can prevent deviation and does not damage the bone structure when scanning mouse ex vivo bones is a technical problem that has not yet been solved in the existing technology. Utility Model Content
[0005] Therefore, the technical problem to be solved by the present invention is to overcome the technical defects in the prior art of using tape to fix bones to the scanning bed, which affects the natural shape of the bones and introduces artifacts, and the use of tape to fix the bones causes direct contact between the bones and the hard surface of the scanning bed, causing damage to the bone structure, thereby affecting the accurate judgment and analysis of the bone structure, thereby providing a disposable bracket for micro-CT scanning of mouse ex vivo limb bones, which can prevent deviation when scanning mouse ex vivo bones and will not damage the bone structure.
[0006] To this end, the utility model provides a disposable bracket for in vitro micro-CT scanning of mouse limb bones, comprising:
[0007] The main body tube is suitable for being fixedly placed on a scanning bed and has an inner cavity suitable for placing the ex vivo limb bones of a mouse, and the inner cavity is at least one;
[0008] a cylinder cover, capable of being connected to the main cylinder cover to seal the inner cavity;
[0009] The limiting and fixing structure is arranged inside the main tube and / or the tube cover, and is used to limit and fix the isolated limb bones of the mouse in the inner cavity.
[0010] As a preferred solution, at least 8 inner cavities are evenly opened inside the main body tube, and the cross section of the inner cavity is circular.
[0011] As a preferred solution, the position limiting and fixing structure includes:
[0012] An inner cavity plug is provided on the inner side of the cylinder cover and can be plugged into the inner cavity;
[0013] A soft cushion is arranged at the bottom of the inner cavity.
[0014] As a preferred solution, after the tube cover is connected to the main tube cover, the inner cavity plug is plugged into the inner cavity, so that the two ends of the isolated mouse limb bones are respectively abutted and fixed to the inner cavity plug and the cushion.
[0015] As a preferred solution, the inner cavity plugs are cylindrical, and at least 8 of them are fixedly arranged on the inner side surface of the cylinder cover.
[0016] As a preferred solution, the bottom surface of the inner cavity plug is provided with a concave cone surface, and the top surface of the cushion is provided with a concave cone surface.
[0017] As a preferred solution, a section of the outer wall near the top of the main body tube is provided with a plurality of annular cutting lines, and the main body tube can be neatly cut at the positions of the annular cutting lines.
[0018] As a preferred solution, a plurality of elastic buckles are provided on the circumferential inner wall of the cylinder cover, and the elastic buckles can be engaged with the annular cutting pattern.
[0019] As a preferred solution, the elastic buckle includes a connecting plate and a claw fixedly arranged at the bottom end of the connecting plate.
[0020] As a preferred solution, two parallel longitudinal openings are provided on the circumferential wall of the cylinder cover, and a transverse opening is connected to the bottom ends of the two longitudinal openings, thereby forming the connecting plate.
[0021] The technical solution provided by the utility model has the following advantages:
[0022] The utility model discloses a disposable bracket for micro-CT scanning of isolated mouse limb bones, comprising a main body tube, a tube cover and a limiting and fixing structure; wherein the main body tube is suitable for being fixedly placed on a scanning bed, and has at least one inner cavity suitable for placing isolated mouse limb bones; the tube cover can be connected to the main body tube cover, thereby sealing the inner cavity; the limiting and fixing structure is arranged inside the main body tube and the tube cover, and is used for limiting and fixing the isolated mouse limb bones in the inner cavity.
[0023] When performing a CT scan on the isolated limb bones of a mouse, the isolated mouse bones are placed in the inner cavity respectively, and then the tube cover is connected to the main tube to seal the inner cavity, so that the mouse bones are fixed between the limiting and fixing structures by the tube cover; after the fixation is completed, the main tube is fixed to the scanning bed to scan and analyze the mouse bones. The utility model is a disposable bracket for micro-CT scanning of isolated limb bones of a mouse. It can limit and fix the mouse bones, and will not deviate during the scanning process. It can ensure that the scanned image is clear and unambiguous, which is convenient for the subsequent accurate judgment and analysis of the bone structure, and the bone structure of the mouse will not be damaged during fixation. In addition, multiple mouse bones can be scanned simultaneously during scanning to improve scanning efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] In order to more clearly illustrate the technical solutions in the prior art or the specific embodiments of the present invention, the following briefly introduces the drawings used in the description of the prior art or the specific embodiments.
[0025] Figure 1 The utility model is a schematic diagram of the overall structure of a disposable bracket used for micro-CT scanning of ex vivo limb bones of mice.
[0026] Figure 2 yes Figure 1 Schematic diagram of the structure of the main cylinder.
[0027] Figure 3 yes Figure 1 Schematic diagram of the structure of the middle tube cover.
[0028] Figure 4 yes Figure 3 Another stereoscopic view of .
[0029] Figure 5 yes Figure 1 Schematic diagram of the structure of the middle cushion.
[0030] Figure numerals: 1, main cylinder; 11, inner cavity; 12, annular cutting pattern; 2, cylinder cover; 3, inner cavity plug; 4, cushion; 5, elastic buckle; 51, connecting plate; 52, claw. DETAILED DESCRIPTION
[0031] In order to enable those skilled in the art to better understand this solution, the following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without making creative efforts should fall within the scope of protection of this application.
[0032] It should be noted that the terms "first," "second," and the like in the claims and description of this application are used to distinguish similar objects, and are not used to describe a particular order or precedence. In addition, the terms "including," "having," and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to those steps or units explicitly listed, but may also include other steps or units not explicitly listed or inherent to such process, method, product, or apparatus.
[0033] In the present application, the terms "upper", "lower", "left", "right", "front", "back", "top", "bottom", "inside", "outside", "middle", "vertical", "horizontal", "lateral", "longitudinal" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. These terms are mainly intended to better describe the present application and its embodiments, and are not intended to limit the indicated devices, elements or components to having a specific orientation, or to be constructed and operated in a specific orientation. Moreover, in addition to being used to indicate orientations or positional relationships, some of the above terms may also be used to indicate other meanings. For example, the term "upper" may also be used to indicate a certain dependency or connection relationship in certain cases. For those skilled in the art, the specific meanings of these terms in the present application can be understood according to the specific circumstances. In addition, the term "multiple" should mean two or more. It should be noted that the embodiments in the present application and the features in the embodiments may be combined with each other unless there is a conflict.
[0034] The present application will be described in detail below with reference to the accompanying drawings and in combination with embodiments. Example
[0035] This embodiment provides a disposable bracket for in vitro micro-CT scanning of mouse limb bones, such as Figure 1-4 As shown, it includes: a main tube 1, a tube cover 2 and a limiting and fixing structure; wherein the main tube 1 is suitable for being fixedly placed on a scanning bed, and has an inner cavity 11 suitable for placing the isolated limb bones of a mouse, and the inner cavity 11 is at least one; the tube cover 2 can be connected to the main tube 1 to cover the inner cavity 11, thereby sealing the inner cavity 11; the limiting and fixing structure is arranged inside the main tube 1 and the tube cover 2, and is used to limit and fix the isolated limb bones of a mouse in the inner cavity 11.
[0036] When performing CT scanning on the isolated limb bones of a mouse, the isolated mouse bones are placed in the inner cavity 11 respectively, and then the tube cover 2 is connected to the main tube 1 to seal the inner cavity 11, so that the mouse bones are fixed between the limiting fixing structures by the tube cover 2; after fixing, the main tube 1 is fixed to the scanning bed to scan and analyze the mouse bones. The disposable bracket of the present embodiment for micro-CT scanning of the isolated limb bones of a mouse can limit and fix the mouse bones, and will not deviate during the scanning process. It can ensure that the scanned image is clear and unambiguous, which is convenient for subsequent accurate judgment and analysis of the bone structure, and the bone structure of the mouse will not be damaged during fixing. In addition, multiple mouse bones can also be scanned simultaneously during scanning to improve scanning efficiency.
[0037] like Figure 2 As shown, at least eight inner cavities 11 are evenly opened inside the main body tube 1, and the cross section of the inner cavity 11 is circular. Opening at least eight inner cavities 11 can meet the needs of scanning multiple mouse bones at the same time and improve scanning efficiency.
[0038] like Figure 3 、 5 As shown, the position-limiting and fixing structure includes an inner cavity plug 3 and a soft cushion 4. The inner cavity plug 3 is disposed on the inner side of the barrel cover 2 and can be plugged into the inner cavity 11. The soft cushion 4 is disposed at the bottom of the inner cavity 11. The inner cavity plug 3 and the soft cushion 4 work together to limit and fix the mouse skeleton without damaging the mouse's skeletal structure.
[0039] After the tube cover 2 is attached to the main tube 1, the inner cavity plug 3 is inserted into the inner cavity 11, so that the two ends of the isolated mouse limb bones are respectively abutted and fixed against the inner cavity plug 3 and the soft cushion 4. The mouse bones are fixed between the inner cavity plug 3 and the soft cushion 4, and will not deviate during the scanning process, so that accurate images can be obtained, thereby enabling precise judgment and analysis.
[0040] like Figure 3As shown, the inner cavity plug 3 is cylindrical, and at least eight of them are fixedly installed on the inner side of the cylinder cover 2. There are at least eight inner cavity plugs 3, the same number as the inner cavity 11. When used together, multiple mouse bones can be scanned simultaneously, improving scanning efficiency, and the mouse bones can be fixed in place by the cooperation.
[0041] The bottom surface of the inner cavity plug 3 is provided with an inwardly concave conical surface, and the top surface of the soft pad 4 is provided with an inwardly concave conical surface. The inwardly concave conical surface design of the top surfaces of the inner cavity plug 3 and the soft pad 4 can ensure that the bone remains stable when subjected to external forces and is not easily displaced.
[0042] like Figure 1 As shown, a section of the outer wall near the top of the main tube 1 is provided with a plurality of annular cutting grooves 12, and the main tube 1 can be neatly cut at the position of the annular cutting grooves 12. When fixing a mouse skeleton, the annular cutting grooves 12 can be cut according to the length of the skeleton to match the skeleton with the main tube 1 and can be fixed in position.
[0043] A plurality of elastic buckles 5 are provided on the inner circumferential wall of the cylinder cover 2, and the elastic buckles 5 can be engaged with the annular cutting groove 12. The elastic buckles 5 are engaged with the annular cutting groove 12 to ensure the stability of the connection.
[0044] The elastic buckle 5 includes a connecting plate 51 and a claw 52 fixedly disposed at the bottom end of the connecting plate 51 .
[0045] The circumferential wall of the cylinder cover 2 is provided with two parallel longitudinal openings and a transverse opening connecting the bottom ends of the two longitudinal openings, thereby forming the connecting plate 51 .
[0046] The disposable bracket for micro-CT scanning of isolated mouse limb bones in this embodiment is used as follows: when performing CT scanning on the isolated mouse limb bones, the isolated mouse bones are placed in the inner cavity 11 respectively, and then the annular cutting pattern 12 is cut according to the length of the bones to match the length of the bones with that of the main tube 1. After cutting, the tube cover 2 is connected to the main tube 1 to seal the inner cavity 11, so that the mouse bones are limited and fixed between the inner cavity plug 3 and the soft cushion 4 through the tube cover 2; after fixing, the main tube 1 is fixed to the scanning bed to scan and analyze the mouse bones.
[0047] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of this innovative technical solution.
Claims
1. A disposable bracket for in vitro micro-CT scanning of mouse limb bones, characterized in that: include: The main body tube (1) is suitable for being fixedly placed on a scanning bed and has an inner cavity (11) suitable for placing the ex vivo limb bones of a mouse, wherein the inner cavity (11) is at least one; A cylinder cover (2) capable of being connected to the main cylinder (1) to seal the inner cavity (11); A position-limiting and fixing structure is provided inside the main cylinder (1) and / or the cylinder cover (2) and is used to position-limit and fix the isolated limb bones of a mouse in the inner cavity (11).
2. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 1, characterized in that: At least eight inner cavities (11) are evenly opened inside the main body tube (1), and the cross section of the inner cavities (11) is circular.
3. The disposable bracket for in vitro micro-CT scanning of mouse limb bones according to claim 1, characterized in that: The position limiting and fixing structure includes: An inner cavity plug (3) is arranged on the inner side of the cylinder cover (2) and can be plugged into the inner cavity (11); A soft cushion (4) is arranged at the bottom of the inner cavity (11).
4. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 3, characterized in that: After the tube cover (2) is connected to the main tube (1), the inner cavity plug (3) is plugged into the inner cavity (11), so that the two ends of the isolated mouse limb bones are respectively abutted and fixed to the inner cavity plug (3) and the soft cushion (4).
5. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 3, characterized in that: The inner cavity plugs (3) are cylindrical, and at least eight of them are fixedly arranged on the inner side surface of the cylinder cover (2).
6. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 3, characterized in that: The bottom surface of the inner cavity plug (3) is provided with a concave conical surface, and the top surface of the soft pad (4) is provided with a concave conical surface.
7. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 1, characterized in that: A section of the outer wall near the top of the main body tube (1) is provided with a plurality of annular cutting lines (12), and the main body tube (1) can be neatly cut at the positions of the annular cutting lines (12).
8. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 7, characterized in that: A plurality of elastic buckles (5) are provided on the circumferential inner wall of the cylinder cover (2), and the elastic buckles (5) can be engaged with the annular cutting pattern (12).
9. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 8, characterized in that: The elastic buckle (5) comprises a connecting plate (51) and a claw (52) fixedly arranged at the bottom end of the connecting plate (51).
10. The disposable holder for in vitro micro-CT scanning of mouse limb bones according to claim 9, characterized in that: Two parallel longitudinal openings are provided on the circumferential wall of the cylinder cover (2), and a transverse opening is provided to connect the bottom ends of the two longitudinal openings, thereby forming the connecting plate (51).