Micro-fluidic chip fluorescence detector

Abstract

The utility model provides a fluorescence detector for a micro-fluidic chip, which belongs to the technical field of micro-fluidic chip detection and comprises a cuboid shell, a rectangular hole is arranged on the side surface of the shell, a touch display screen is arranged on the shell, a groove for placing a chip to be detected is arranged in the shell, and the chip to be detected is arranged in the groove. The tray is used for placing the groove; the motor sliding rail is used for placing the tray; the driving part is used for driving the motor and the to-be-detected chip to rotate so as to separate the to-be-detected sample into the detection groove; the fluorescence module is used for emitting detection light to the to-be-detected sample and receiving fluorescence generated by detection; the main control board is used for acquiring a fluorescence signal and analyzing and processing data; the temperature control module is used for heating and radiating the sample to be detected; the touch display screen, the driving piece, the fluorescent module and the temperature control module are electrically connected with the main control board. The rapid detection system is simple to operate, can be used for rapidly detecting a sample to be detected, and aims to solve the problems of complex operation and poor portability of the conventional detection system.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic chip detection, in particular to a microfluidic chip fluorescence detector. Background Art

[0002] Microfluidics refers to the science and technology of precisely controlling and manipulating fluids at small, even microscale, scales. It implements sample pretreatment, reaction, and analysis steps that can only be performed in a standard laboratory within a chip measuring a few square centimeters, hence the name microfluidic chip. Compared to other fluid control devices, microfluidic chips, due to their microscale characteristics, significantly improve microfluidic contact efficiency and enhance heat transfer efficiency. They also significantly reduce reagent and sample volume consumption, facilitating parallel analysis in confined environments. They can be applied to scenarios such as large-scale screening, batch sample processing, or mobile testing.

[0003] Microfluidic chips are a hot topic in the development of micro-volume full-analysis systems. By combining inorganic, organic, and biological reactions, they complete the entire process, including reagent addition, sample separation, reaction, and result detection, or part of the process, into a micron-scale structure and integrated on a chip of several square centimeters. This technology provides multiple reaction chambers on a chip, enabling multiple tests or analysis of multiple detection targets. In the field of point-of-care diagnostics (POCT), microfluidic technology has broad application prospects and application value in a series of analytical fields such as biochemical indicator analysis, DNA analysis, immunoassay, and toxicity testing. However, common microfluidic detection systems have disadvantages such as complex control, low degree of automation, high cost, inability to be applied on a large scale, and poor portability.

[0004] Therefore, how to provide a microfluidic detection method that is simple to operate, highly intelligent, and portable has become a technical problem that needs to be urgently solved by those skilled in the art. Utility Model Content

[0005] The purpose of the utility model is to provide a microfluidic chip fluorescence detector which is simple to operate, highly intelligent and portable.

[0006] The utility model provides a microfluidic chip fluorescence detector, comprising a housing,

[0007] A rectangular hole is provided on the side of the shell, and a touch display screen is provided on the shell;

[0008] The housing is provided with a groove for placing the chip to be tested, the housing is provided with a tray for placing the groove, and the housing is provided with a motor slide rail for placing the tray;

[0009] A driving member is provided in the housing, and the driving member is used to drive the motor and the chip to be tested to rotate so that the sample to be tested is separated and enters each detection slot of the chip;

[0010] and a fluorescence module, the fluorescence module being used to emit fluorescence to the sample to be detected and receive the processed and amplified fluorescence signal;

[0011] and a main control board, which acquires the processed and amplified fluorescent signal and performs data analysis to obtain a detection result;

[0012] and a temperature control module, which can quickly heat the temperature of the sample to be tested to reach the reaction conditions, and can quickly reduce the temperature of the sample to be tested and the components in the housing;

[0013] The touch display screen, the driving component, the fluorescent module, and the temperature control module are electrically connected to the main control board respectively.

[0014] Furthermore, the motor slide rail is placed on the slide rail support, and the tray can place the chip to be tested through the rectangular hole on one side of the shell under the action of the motor slide rail. A heating plate is provided under the groove, and the diameter of the heating plate is smaller than the diameter of the groove, and the sample to be tested is heated by the heating plate.

[0015] Furthermore, the motor is provided with a coupling, the output shaft of the motor is connected to the coupling, the coupling is fixedly connected to a chuck, and the chuck is adapted to a slot at the center of the chip to be detected.

[0016] Furthermore, the chuck can be driven by the motor to move upward until it is fixedly nested with the center slot of the chip to be tested in the groove.

[0017] Furthermore, the fluorescence module includes a fluorescence excitation unit, a fluorescence processing unit and a receiving detection unit. The fluorescence excitation unit emits fluorescence to the sample to be detected under the control of the main control board. The fluorescence processing unit filters and amplifies the output fluorescence formed after the sample to be detected is irradiated by fluorescence. The receiving detection unit receives the processed and amplified fluorescence signal, and the receiving detection unit sends the received fluorescence signal to the main control board.

[0018] Furthermore, the temperature control module includes a fan, a liquid pump and a copper tube arranged on one side of the shell. The fan is electrically connected to the main control board, and the liquid pump is electrically connected to the main control board. A bidirectional heating rod is provided in the liquid pump, and the liquid pump transports liquid to the copper tube to achieve heating or heat dissipation functions.

[0019] Furthermore, a bottom plate is provided in the shell, and the driving component, motor and fluorescent module are installed on the bottom plate.

[0020] Furthermore, the shell includes an upper shell and a lower shell that are spliced ​​together to facilitate installation and disassembly.

[0021] Compared with the prior art, the present invention has the following advantages:

[0022] The microfluidic chip fluorescence detector has a rectangular hole on one side of the shell, and a motor slide is provided inside the shell. A tray with a groove is placed on the motor slide. The tray can pass through the rectangular hole under the sliding of the motor slide. The chip containing the sample to be tested is placed in the groove, and the tray is pushed back. Then, the automatic detection can be realized by operating on the touch screen. The operation is convenient and simple.

[0023] The microfluidic chip fluorescence detector is small in size and has good portability. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0025] Figure 2 This is an exploded view of the overall structure of the utility model;

[0026] Figure 3 It is a side perspective view of the overall structure of the utility model;

[0027] Figure 4 This is a schematic diagram of the structure of the chip to be detected in the present invention;

[0028] Description of Reference Numerals

[0029] 1.1. Upper housing; 1.2. Lower housing; 2. Touch screen; 3. Main control board; 4. Groove; 5. Tray; 6. Motor slide rail; 7. Heating plate; 8. Slide rail support; 9. Chuck; 10. Motor; 11. Fluorescent module; 12. Temperature control module; 13. Drive component; 14. Rectangular hole; 15. Bottom plate; 16. Chip to be tested; 16.1. Card slot. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] like Figures 1 to 4 As shown, the utility model shows a microfluidic chip fluorescence detector, including a housing,

[0032] A rectangular hole 14 is provided on the right side of the housing, and a touch display screen 2 is provided on the housing;

[0033] The housing is provided with a groove 4 for placing the chip 16 to be tested, a tray 5 for placing the groove 4, and a motor slide rail 6 for placing the tray 5;

[0034] The housing is further provided with a driving member 13 for driving the motor 10 and the test chip 16 to rotate so that the test sample is separated and enters each test slot of the chip; a fluorescence module 11 for emitting fluorescence to the test sample and receiving the processed and amplified fluorescence signal; a main control board 3 for acquiring the processed and amplified fluorescence signal and analyzing the data to obtain the test result; and a temperature control module 12 for rapidly heating the test sample to the reaction condition and rapidly reducing the temperature of the test sample and the components in the housing.

[0035] The touch display screen 2 , the driving component 13 , the fluorescent module 11 , and the temperature control module 12 are electrically connected to the main control board 3 , respectively.

[0036] This utility model designs a small, portable microfluidic chip fluorescence detector. The microfluidic chip fluorescence detector has a rectangular hole 14 on one side of the housing. A motor slide 6 is provided inside the housing. A tray 5 with grooves 4 is placed on the motor slide 6. The tray 5 slides through the rectangular hole 14 under the sliding motion of the motor slide 6. Sixteen cores to be tested are placed in the grooves 4, pushed back into the tray, and then operated on the touch screen 2 to achieve automated testing. Operation is simple and convenient.

[0037] Specifically, such as Figure 2 The motor slide rail 6 is placed on the slide rail support 8. Under the action of the motor slide rail 6, the tray 5 can place the chip 16 to be tested through the rectangular hole 14. A heating plate 7 is provided under the groove 4. The diameter of the heating plate 7 is smaller than the diameter of the groove 4.

[0038] The motor 10 is provided with a coupling, the output shaft of the motor 10 is connected to the coupling, the coupling is fixedly connected to the chuck 9, and the chuck 9 is adapted to the card slot 16.1 at the center position of the chip to be detected 16.

[0039] Furthermore, the chuck 9 can be driven by the motor 10 to move upward until it is fixedly nested with the center slot 16 . 1 of the chip to be tested 16 in the groove 4 .

[0040] The fluorescence module 11 includes a fluorescence excitation unit, a fluorescence processing unit and a receiving detection unit. The fluorescence excitation unit emits fluorescence to the sample to be detected under the control of the main control board 3. The fluorescence processing unit filters and amplifies the output fluorescence formed after the sample to be detected is irradiated by fluorescence. The receiving detection unit receives the processed and amplified fluorescence signal, and the receiving detection unit sends the received fluorescence signal to the main control board 3.

[0041] The temperature control module 12 includes a fan, a liquid pump 12.1 and a copper tube arranged on one side of the shell. The fan is electrically connected to the main control board 3, and the liquid pump 12.1 is electrically connected to the main control board 3. The liquid pump 12.1 is provided with a bidirectional heating and cooling rod and can transport heating liquid or cooling liquid to the copper tube, thereby achieving rapid heating of the sample to be tested and rapid heat dissipation of the sample to be tested and components in the shell.

[0042] A bottom plate 15 is provided in the housing, and the driving component 13 , the motor 10 , and the fluorescent module 11 are mounted on the bottom plate 15 .

[0043] The housing comprises an upper housing 1.1 and a lower housing 1.2 which are spliced ​​together.

[0044] Working principle: During the specific test, add the sample to be tested into the test chip, then touch the icon for removing the tray on the touch screen 2, put the chip to be tested 16 into the groove 4, touch the icon for pushing the tray back on the touch screen 2, and then touch the icon for starting the test to start the automated test. During the test, the main control board 3 drives the motor 10 to raise the chuck 9 to nest with the center slot 16.1 of the chip to be tested 16, and the heating plate 7 heats the chip to be tested 16. At the same time, the bidirectional heating and cooling rod in the liquid pump 12.1 starts to heat the liquid and transports the heated liquid to the copper tube, so that the chip to be tested 16 is under constant temperature control. The main control board 3 controls the motor 10 to rotate, driving the chuck 9 to rotate, thereby driving the chip to be tested 16 to rotate. The sample to be tested in the chip to be tested 16 is separated by centrifugal force and enters the test tank. After the sample to be tested has completely entered the test tank, After the temperature of the detection tank of the chip to be detected 16 reaches the reaction requirement, the fluorescence excitation unit starts to emit fluorescence with a wavelength of 470nm. The fluorescence excites the outgoing fluorescence after it shines on the sample to be detected in the detection tank. The outgoing fluorescence passes through the fluorescence processing unit and is received by the receiving detection unit. After reception, the data is transmitted to the main control board 3. The main control board 3 analyzes and processes the received data to obtain the detection and judgment results, and displays the detection structure through the touch screen 2. After the detection is completed, the bidirectional heating and cooling rod in the liquid pump 12.1 starts to transport coolant to the copper tube to dissipate heat for the sample to be tested and the components in the shell.

[0045] Therefore, the utility model is simple to operate and highly intelligent, and has no special requirements on the operator's operating level. It only requires adding the sample to be tested into the chip, and the chip is fully automated from centrifugation to detection with high efficiency. In addition, the instrument is small in size, light in weight, and easy to carry.

[0046] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, inside, outside, etc.) are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0047] In this utility model, unless otherwise specified or limited, the terms "installation," "connection," "fixation," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0048] Furthermore, it should be understood that when the terms “include” and / or “comprise” are used in this specification, they specify the presence of features, steps, operations, devices, components and / or their combinations.

[0049] The above are only preferred embodiments of the present invention and do not limit the implementation methods and protection scope of the present invention. Those skilled in the art should be aware that all solutions obtained by equivalent substitutions and obvious changes made using the description and illustrations of the present invention should be included in the protection scope of the present invention.

Claims

1. A microfluidic chip fluorescence detector, comprising a housing, characterized in that: A rectangular hole (14) is provided on the side of the shell, and a touch display screen (2) is provided on the shell; A groove (4) for placing a chip (16) to be detected is provided in the housing, a tray (5) for placing the groove (4) is provided in the housing, and a motor slide rail (6) for placing the tray (5) is provided in the housing; A driving member (13) is provided in the housing, and the driving member (13) is used to drive the motor (10) and the chip to be detected (16) to rotate so that the sample to be detected is separated and enters each detection slot of the chip; and a fluorescence module (11), wherein the fluorescence module (11) is used to emit fluorescence to the sample to be detected and receive the processed and amplified fluorescence signal; and a main control board (3), wherein the main control board (3) obtains the processed and amplified fluorescence signal and performs data analysis and processing to obtain a detection result; and a temperature control module (12), wherein the temperature control module (12) is capable of rapidly heating the temperature of the sample to be detected to reach reaction conditions, and is capable of rapidly reducing the temperature of the sample to be detected and components within the housing; The touch display screen (2), the driving component (13), the fluorescent module (11), and the temperature control module (12) are electrically connected to the main control board (3) respectively.

2. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The motor slide rail (6) is placed on the slide rail support (8), and the tray (5) can place the chip (16) to be tested through the rectangular hole (14) under the action of the motor slide rail (6). A heating plate (7) is provided below the groove (4), and the diameter of the heating plate (7) is smaller than the diameter of the groove (4).

3. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The motor (10) is provided with a coupling, the output shaft of the motor (10) is connected to the coupling, the coupling is fixedly connected to the chuck (9), and the chuck (9) is adapted to the card slot (16.1) at the center position of the chip to be detected (16).

4. The microfluidic chip fluorescence detector according to claim 3, characterized in that: The chuck (9) can be driven by the motor (10) to move upward until it is fixedly nested with the center slot (16.1) of the chip to be tested (16) in the groove (4).

5. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The fluorescence module (11) comprises a fluorescence excitation unit, a fluorescence processing unit and a receiving detection unit. The fluorescence excitation unit emits fluorescence to the sample to be detected under the control of the main control board (3). The fluorescence processing unit performs filtering and amplification processing on the outgoing fluorescence formed by the sample to be detected after fluorescence irradiation. The receiving detection unit receives the processed and amplified fluorescence signal, and the receiving detection unit sends the received fluorescence signal to the main control board (3).

6. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The temperature control module (12) comprises a fan, a liquid pump (12.1) and a copper tube arranged on one side of the housing; the fan is electrically connected to the main control board (3); the liquid pump (12.1) is electrically connected to the main control board (3); and a bidirectional heating and cooling rod is provided in the liquid pump (12.1).

7. The microfluidic chip fluorescence detector according to claim 1, characterized in that: A base plate (15) is also provided in the housing, and the driving member (13), the motor (10) and the fluorescent module (11) are mounted on the base plate (15).

8. The microfluidic chip fluorescence detector according to claim 1, characterized in that: The shell comprises an upper shell (1.1) and a lower shell (1.2) that are spliced ​​together.

Images