Umbilical cord stem cell exosome low-temperature preservation device

Abstract

The utility model discloses an umbilical cord stem cell exosome low-temperature preservation device, and relates to the technical field of exosome assistance. The semiconductor refrigeration box comprises a box body, a vertical cylindrical positioning cavity is formed in the box body, at least three heat dissipation plates arranged around the axis of the positioning cavity are formed in the positioning cavity, a semiconductor refrigeration device is arranged between every two adjacent heat dissipation plates, the heat dissipation plates are attached to the hot end of the semiconductor refrigeration device, and the heat dissipation plates are arranged in the positioning cavity. A plurality of cooling air ducts are constructed on the binding face of the heat dissipation plate and the semiconductor refrigeration device, a heat dissipation fan blowing air towards the heat dissipation plate is installed on the bottom face of the box body, and a storage pipe used for containing exosomes is constructed in the semiconductor refrigeration device; the purposes of providing a more stable preservation environment for the umbilical cord stem cell exosome and ensuring effectiveness are achieved.

Description

Technical Field

[0001] The utility model relates to the field of exosome-assisted technology, and specifically, is a low-temperature storage device for umbilical cord stem cell exosomes. Background Art

[0002] In recent years, with the rapid development of stem cell regenerative medicine, exosomes, tiny vesicles secreted by stem cells, have gradually become a hot topic in the medical community and are being used in clinical trials for a variety of diseases. Although a growing number of scientific studies have confirmed the effectiveness of exosomes in clinical applications, the stability and functionality of exosomes remain crucial, and have become key factors in evaluating the quality of exosome products.

[0003] Currently, a large number of studies have shown that although exosomes are recommended to be stored at -80°C or -20°C, it is sometimes difficult to maintain such low temperature conditions during actual use. They can basically only be used or transported in a foam box mixed with ice packs and stored in commercial refrigerators. However, the temperature does not reach the optimal storage temperature of the exosomes themselves, resulting in the destruction of the integrity of the vesicles themselves and a rapid decrease in the effective components of the exosomes. Utility Model Content

[0004] The purpose of the utility model is to provide a low-temperature storage device for umbilical cord stem cell exosomes, so as to provide a more stable storage environment for umbilical cord stem cell exosomes and ensure their effectiveness.

[0005] In order to achieve the above purpose, the utility model adopts the following technical means:

[0006] A device for cryopreservation of umbilical cord stem cell exosomes comprises a housing having a vertical cylindrical positioning cavity therein, the positioning cavity containing at least three heat sinks arranged around the axis of the positioning cavity, a semiconductor refrigeration device disposed between two adjacent heat sinks, the heat sinks affixed to the hot ends of the semiconductor refrigeration devices, a plurality of cooling air ducts being configured on the affixed surfaces of the heat sinks and the semiconductor refrigeration devices, a cooling fan for blowing air toward the heat sinks mounted on the bottom surface of the housing, and a storage tube for placing exosomes within the semiconductor refrigeration device.

[0007] Preferably, two adjacent heat dissipation plates are arranged at equal distances.

[0008] Furthermore, the cooling air duct includes a first air duct provided on the surface of the heat dissipation plate and parallel to the axis of the positioning cavity, and a second air duct provided on the surface of the heat dissipation plate and perpendicular to the axis of the positioning cavity.

[0009] Furthermore, the first air ducts are respectively arranged on both sides of the heat dissipation plate, and the two adjacent first air ducts on both sides of the heat dissipation plate are arranged in a staggered direction.

[0010] Furthermore, the second air ducts are arranged on both sides of the heat dissipation plate, and the two adjacent second air ducts on both sides of the heat dissipation plate are arranged in a staggered direction.

[0011] Furthermore, the box body is constructed with a plurality of horizontally penetrating ventilation holes.

[0012] Furthermore, the storage tube includes a first holding tube, a second holding tube and a third holding tube with gradually decreasing inner diameters, the third holding tube is coaxially arranged in the second holding tube, the second holding tube is coaxially arranged in the first holding tube, the pipe mouth of the first holding tube is installed with a first stopper, the pipe mouth of the second holding tube is installed with a second stopper, and the pipe mouth of the third holding tube is installed with a third stopper, the first stopper, the second stopper and the third stopper are placed one above the other from bottom to top, and the first stopper is placed against the top surface of the semiconductor refrigeration device.

[0013] During use, the utility model has the following beneficial effects:

[0014] The exosomes in the storage tank are cryopreserved by using a semiconductor refrigeration device. Furthermore, a heat sink, combined with a cooling air duct installed on the heat sink, allows the airflow from the cooling fan to flow upward through the cooling air duct, thereby continuously cooling the hot end of the semiconductor refrigeration device using the heat sink. This prevents the temperature difference between the hot and cold ends of the semiconductor refrigeration device from decreasing, resulting in the forward and reverse heat transfer canceling each other out and preventing further cooling. The heat sink with a cooling air duct, combined with a cooling fan for blasting cooling air, can dissipate heat from the hot end efficiently, quickly, and stably. This effectively lowers the temperature of the hot end through active heat dissipation, allowing the cold end to reach the storage temperature of the exosomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic structural diagram of the utility model.

[0016] Figure 2 This is a schematic diagram of the installation structure of the heat sink and semiconductor refrigeration device of the utility model.

[0017] Figure 3 This is a schematic diagram of the heat dissipation plate structure of the utility model.

[0018] Figure 4 for Figure 3 Schematic diagram of the locally enlarged structure at point A in the middle.

[0019] Figure 5 for Figure 3Schematic diagram of the top view structure.

[0020] Figure 6 It is a structural schematic diagram of the storage tank of the utility model.

[0021] Figure 7 for Figure 6 Schematic diagram of the cross-section structure.

[0022] Among them, 1-box, 2-positioning cavity, 3-heat sink, 4-semiconductor refrigeration device, 5-storage pipe, 6-first air duct, 7-second air duct, 8-ventilation hole, 9-first holding pipe, 10-second holding pipe, 11-third holding pipe, 12-first stopper, 13-second stopper, 14-third stopper. DETAILED DESCRIPTION

[0023] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0024] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but rather merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0025] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0026] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0027] In the description of the present invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the product of the present invention is typically placed when in use, or are the orientations or positional relationships commonly understood by those skilled in the art. These terms are intended solely to facilitate the description of the present invention and to simplify the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used solely to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0028] It should also be noted that, in the description of this utility model, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0029] Please refer to Figures 1 to 7 As shown, a device for cryopreservation of umbilical cord stem cell exosomes includes a housing 1, wherein a vertical cylindrical positioning cavity 2 is constructed therein, wherein at least three heat sinks 3 are constructed within the positioning cavity 2 and are arranged around the axis of the positioning cavity 2. A semiconductor refrigeration device 4 is provided between two adjacent heat sinks 3, wherein the heat sink 3 is bonded to the hot end of the semiconductor refrigeration device 4, and a plurality of cooling air ducts are constructed between the bonding surfaces of the heat sink 3 and the semiconductor refrigeration device 4. A cooling fan for blowing air toward the heat sink 3 is installed on the bottom surface of the housing 1, and a storage tube 5 for placing exosomes is constructed within the semiconductor refrigeration device 4.

[0030] In this way, the semiconductor refrigeration device 4 is used to preserve the exosomes in the storage tank at low temperatures. Furthermore, the heat sink 3, combined with the cooling air duct provided on the heat sink 3, allows the airflow from the cooling fan to flow upward through the cooling air duct, thereby continuously cooling the hot end of the semiconductor refrigeration device 4 using the heat sink 3. This prevents the temperature difference between the hot and cold ends of the semiconductor refrigeration device 4 from decreasing, which would cause the forward and reverse heat transfer to cancel each other out and prevent further cooling. The heat sink 3 with its cooling air duct, combined with the cooling fan for dissipating the cooling air, can efficiently, quickly, and stably dissipate heat from the hot end. This effectively lowers the temperature of the hot end through active heat dissipation, allowing the cold end to reach the exosome storage temperature.

[0031] Among them, a battery can be set in the box body 1, and the battery is used to supply power to the cooling fan and the semiconductor refrigeration device 4, so that the entire device can be used even when the power is off and is not affected by power outages.

[0032] Furthermore, two adjacent heat dissipation plates 3 are arranged at equal distances.

[0033] As a result, the semiconductor refrigeration devices 4 sandwiched between the two heat sinks 3 have the same volume, making it easier to arrange the semiconductor refrigeration devices 4 with the same cooling effect.

[0034] Furthermore, the cooling air duct includes a first air duct 6 provided on the surface of the heat dissipation plate 3 and parallel to the axis of the positioning cavity 2 , and a second air duct 7 provided on the surface of the heat dissipation plate 3 and perpendicular to the axis of the positioning cavity 2 .

[0035] Moreover, the first air ducts 6 are respectively arranged on both sides of the heat dissipation plate 3 , and the two adjacent first air ducts 6 on both sides of the heat dissipation plate 3 are staggered.

[0036] At the same time, the second air ducts 7 are arranged on both sides of the heat dissipation plate 3 , and the two adjacent second air ducts 7 on both sides of the heat dissipation plate 3 are staggered.

[0037] Thus, the aforementioned arrangement of the cooling air ducts, through the provision of the first air duct 6 and the second air duct 7, destroys the fluid boundary layer, increases the fluid flow distance within the tube, and the fin effect greatly improves the heat transfer efficiency, thereby further efficiently cooling the hot end of the semiconductor refrigeration device 4.

[0038] Furthermore, the box body 1 is constructed with a plurality of horizontally penetrating ventilation holes 8 .

[0039] In addition, the storage tube 5 includes a first holding tube 9, a second holding tube 10 and a third holding tube 11 with gradually decreasing inner diameters. The third holding tube 11 is coaxially arranged in the second holding tube 10, and the second holding tube 10 is coaxially arranged in the first holding tube 9. A first stopper 12 is installed at the pipe mouth of the first holding tube 9, a second stopper 13 is installed at the pipe mouth of the second holding tube 10, and a third stopper 14 is installed at the pipe mouth of the third holding tube 11. The first stopper 12, the second stopper 13 and the third stopper 14 are placed one above the other from bottom to top, and the first stopper 12 is placed against the top surface of the semiconductor refrigeration device 4.

[0040] Thus, centrifuge tubes of various sizes can be directly placed. It is only necessary to remove the storage tube 5 of the corresponding inner diameter and then put the corresponding centrifuge tube in, so that the centrifuge tube can be placed in the semiconductor refrigeration device 4 for cooling.

[0041] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cryopreservation device for umbilical cord stem cell exosomes, characterized in that: The invention comprises a box body (1), wherein a vertical cylindrical positioning cavity (2) is constructed in the box body (1), wherein at least three heat dissipation plates (3) arranged around the axis of the positioning cavity (2) are constructed in the positioning cavity (2), a semiconductor refrigeration device (4) is provided between two adjacent heat dissipation plates (3), wherein the heat dissipation plates (3) are bonded to the hot end of the semiconductor refrigeration device (4), and a plurality of cooling air ducts are constructed on the bonding surface of the heat dissipation plates (3) and the semiconductor refrigeration device (4), and a heat dissipation fan for blowing air toward the heat dissipation plates (3) is installed on the bottom surface of the box body (1), and a storage tube (5) for placing exosomes is constructed in the semiconductor refrigeration device (4).

2. The umbilical cord stem cell exosome cryopreservation device according to claim 1, characterized in that: The two adjacent heat dissipation plates (3) are arranged at equal distances.

3. The umbilical cord stem cell exosome cryopreservation device according to claim 1, characterized in that: The cooling air duct comprises a first air duct (6) provided on the surface of the heat dissipation plate (3) and parallel to the axis of the positioning cavity (2), and a second air duct (7) provided on the surface of the heat dissipation plate (3) and perpendicular to the axis of the positioning cavity (2).

4. The umbilical cord stem cell exosome cryopreservation device according to claim 3, characterized in that: The first air ducts (6) are respectively arranged on both sides of the heat dissipation plate (3), and two adjacent first air ducts (6) on both sides of the heat dissipation plate (3) are staggered.

5. The umbilical cord stem cell exosome cryopreservation device according to claim 3, characterized in that: The second air ducts (7) are arranged on both sides of the heat dissipation plate (3), and two adjacent second air ducts (7) on both sides of the heat dissipation plate (3) are staggered.

6. The umbilical cord stem cell exosome cryopreservation device according to claim 1, characterized in that: The box body (1) is provided with a plurality of horizontally penetrating ventilation holes (8).

7. The umbilical cord stem cell exosome cryopreservation device according to claim 1, characterized in that: The storage tube (5) comprises a first holding tube (9), a second holding tube (10) and a third holding tube (11) with gradually decreasing inner diameters, the third holding tube (11) being coaxially arranged in the second holding tube (10), the second holding tube (10) being coaxially arranged in the first holding tube (9), a first stopper (12) being installed at the tube mouth of the first holding tube (9), a second stopper (13) being installed at the tube mouth of the second holding tube (10), and a third stopper (14) being installed at the tube mouth of the third holding tube (11), the first stopper (12), the second stopper (13) and the third stopper (14) being placed in an overlapping manner from bottom to top, and the first stopper (12) being placed against the top surface of the semiconductor refrigeration device (4).

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