Device for observing and recording mating behaviors of drosophila melanogaster

Abstract

The utility model discloses a device for observing and recording the mating behavior of drosophila melanogaster relates to drosophila melanogaster behavioristics field, a feeding part comprises a front side plate, a rear side plate, a bottom plate, a feeding plate and a cover plate, the bottom plate is arranged between the front side plate and the rear side plate, the whole of the front side plate, the rear side plate and the bottom plate is H-shaped, vent holes are uniformly arranged on the bottom plate, and the cover plate is arranged on the front side plate and the rear side plate. Cotton white paper is placed on the surface of the bottom plate, the feeding plate is placed on the cotton white paper, a plurality of staggered feeding holes are formed in the feeding plate, the feeding plate is covered with the cover plate, the L-shaped plate is installed on the rear side plate, an observation hole is formed in the L-shaped plate, and the motion camera is arranged at the observation hole. The device is simple in structure and low in cost, facilitates focusing of the camera, and can realize long-time observation and recording of mating behaviors of multiple pairs of drosophila melanogaster.

Description

Technical Field

[0001] The utility model belongs to the field of fruit fly behavior, and in particular relates to a device for observing and recording the mating behavior of fruit flies. Background Art

[0002] The study of Drosophila mating behavior is not only of great significance in genetic research, but also plays an important role in many fields such as neuroscience, medicine and ecology.

[0003] As a classic model for genetic research, the mating behavior of fruit flies can help scientists better understand the laws of inheritance and gene expression. The small number of chromosomes in fruit flies makes genetic manipulation relatively simple, facilitating gene editing and genetic analysis. By observing the mating behavior of fruit flies, scientists can explore various mechanisms in the reproduction process of organisms, including mating choice and the impact of mating frequency on reproductive success. The mating behavior of fruit flies can also be used to study neuroscience and reward mechanisms. By stimulating neurons in the fruit fly brain, scientists found that when the dopamine neuron group is stimulated, the fruit fly will mate endlessly for several hours, indicating that stimulating neurons can generate motivation to do things. In addition, fruit flies are used to diagnose a large number of rare genetic diseases. Due to its short life history, easy to raise, and fast reproduction, the fruit fly model is of great value in medical research.

[0004] However, existing fruit fly rearing devices are typically only used for rearing and observing a single pair of fruit flies. For example, utility model patent CN221011546U discloses a fruit fly rearing device that facilitates observation. However, this device has a complex structure and is not suitable for photographing and recording the mating behavior of fruit flies. Conventional fruit fly culture boxes are difficult to fix and focus the camera when recording with a camera, and a special camera bracket is required. However, the presence of the camera bracket may affect the assembly of the fruit flies, especially when recording the mating behavior of multiple groups of fruit flies simultaneously, which is very inconvenient. There is no device specifically designed for observing and recording the mating behavior of fruit flies for a long time.

[0005] Therefore, how to provide a device that is convenient for observing and recording the mating behavior of fruit flies for a long time has become a problem that needs to be solved by those skilled in the art. Utility Model Content

[0006] In view of this, the present invention provides a device for observing and recording the mating behavior of fruit flies. The present invention has a simple structure, low cost, facilitates the focusing of a motion camera, and can simultaneously observe multiple pairs of fruit flies for a long time.

[0007] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0008] A device for observing and recording the mating behavior of fruit flies comprises a feeding section, an L-shaped plate and a motion camera, wherein the feeding section comprises a front side plate, a rear side plate, a bottom plate, a feeding plate and a cover plate, wherein the bottom plate is arranged between the front side plate and the rear side plate, and the front side plate, the rear side plate and the bottom plate are H-shaped as a whole, exhaust holes are evenly arranged on the bottom plate, white cotton paper is placed on the surface of the bottom plate, the feeding plate is placed on the white cotton paper, a plurality of feeding holes arranged in a staggered manner are opened on the feeding plate, the cover plate is installed on the feeding plate, the L-shaped plate is installed on the rear side plate, an observation hole is opened on the L-shaped plate, and the motion camera is arranged at the observation hole.

[0009] Furthermore, an adjusting bolt is provided on the back of the rear side plate, and an adjusting hole is provided at the assembly position of the L-shaped plate corresponding to the rear side plate, for adjusting the installation height of the L-shaped plate.

[0010] Furthermore, anti-falling stabilizing plates are provided at the bottom of the front side plates and the rear side plates, and the front side plates and the rear side plates are vertically arranged on the anti-falling stabilizing plates.

[0011] Furthermore, the feeding part, L-shaped plate and anti-falling stabilizing plate are all made of transparent acrylic.

[0012] Furthermore, the length of the front side plate and the rear side plate are both 180 mm, the thickness of both are 3 mm, the width of the front side plate is 25 mm, and the width of the rear side plate is 40 mm.

[0013] Furthermore, the length and width of the bottom plate, feeding plate and cover plate are exactly the same, which are 180 mm and 100 mm respectively. The thickness of the bottom plate and cover plate are both 3 mm, and the thickness of the feeding plate is 5 mm.

[0014] Furthermore, the feeding holes on the feeding plate are provided with two rows, the two rows of feeding holes are staggered, the row spacing between the two rows of feeding holes is 30 mm, and the spacing between feeding holes in the same row is 15 mm.

[0015] Furthermore, the diameter of the feeding hole is 15 mm.

[0016] The beneficial effects of the present invention are as follows: the present invention is manufactured by splicing acrylic boards, has a simple structure, and has a low manufacturing cost. The arrangement of the bottom plate, the feeding plate, and the cover plate facilitates the feeding and observation of multiple pairs of fruit flies. The arrangement of the L-shaped plate is convenient for the placement of the motion camera without affecting the assembly of the fruit flies and the cover plate. At the same time, the height can also be adjusted. Combined with the fine-tuning of the feeding plate between the front and rear side plates and the adjustment of the focal length of the motion camera, all feeding holes can be included in the lens, which is conducive to the long-term shooting and recording of the mating behavior of fruit flies, and can also be disassembled when not recording. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1This is a schematic diagram of the main structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the bottom plate position of the utility model;

[0019] Figure 3 This is a schematic diagram of the positional relationship among the bottom plate, feeding plate and cover plate of the utility model;

[0020] Figure 4 This is a schematic diagram of the installation of the L-shaped plate of the utility model.

[0021] In the picture:

[0022] 1. Front side panel; 2. Rear side panel; 3. Bottom panel; 4. Feeding plate; 5. Cover plate; 6. L-shaped plate; 7. Action camera; 8. Adjustment bolts; 9. Feeding holes; 10. Anti-fall stabilization plate. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0024] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "horizontal", "inner", "outer", "one side", etc., indicating directions or positional relationships, are directions or positional relationships based on the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or components referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention; the terms "first", "second", and "third" are only used for descriptive purposes and should not be understood as indicating or implying relative importance. In addition, unless otherwise expressly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or a circuit connection; it can be a direct connection, an indirect connection through an intermediate medium, or it can be internal communication between two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] Example 1

[0026] like Figure 1-4As shown, this embodiment discloses a device for observing and recording the mating behavior of fruit flies, including a feeding section, an L-shaped plate 6, and a motion camera 7. The feeding section includes a front panel 1, a rear panel 2, a bottom panel 3, a feeding plate 4, and a cover panel 5. The bottom panel 3 is arranged between the front panel 1 and the rear panel 2. The bottom panel 3, the front panel 1, and the rear panel 2 form an H-shape. The bottom panel 3 is evenly arranged with exhaust holes for ventilation and oxygen supply to the fruit flies. The surface of the bottom panel 3 is placed with cotton white paper, which serves as a solid color background to facilitate observation of the fruit flies without affecting their breathing. The feeding plate 4 is placed on the white paper. In this embodiment, the feeding plate 4 is provided with several feeding holes 9, each of which is numbered. The cover panel 5 is mounted on the feeding plate 4. By providing multiple feeding holes, the mating of multiple pairs of fruit flies can be recorded. The L-shaped plate 6 is mounted on the rear panel 2, and an observation hole is opened on the L-shaped plate 6. The motion camera 7 is placed at the observation hole to record the behavior of the fruit flies.

[0027] As a preferred embodiment of the present invention, an anti-falling stabilization plate 10 is provided at the bottom of the front side plate 1 and the rear side plate 2, and the front side plate 1 and the rear side plate 2 are vertically arranged on the anti-falling stabilization plate 10 to improve the stability of the device placement.

[0028] Specifically, the feeding part, L-shaped plate 6 and anti-falling stabilizing plate 10 are all made of transparent acrylic material, the length of the front side plate 1 and the rear side plate 2 are both 180 mm, the thickness is 3 mm, the width of the front side plate 1 is 25 mm, and the width of the rear side plate 2 is 40 mm.

[0029] The length and width of the bottom plate 3, the feeding plate 4 and the cover plate 5 are exactly the same, which are 180 mm and 100 mm respectively. The thickness of the bottom plate 3 and the cover plate 5 are both 3 mm, and the thickness of the feeding plate 4 is 5 mm.

[0030] As a preferred embodiment of the present invention, the feeding holes 9 on the feeding plate 4 are arranged in two rows with 8 holes, and the two rows of feeding holes 9 are staggered to facilitate the covering of the feeding holes 9 in sequence when the cover plate 5 is pushed. The row spacing of the two rows of feeding holes 9 is 30 mm, the spacing of the feeding holes 9 in the same row is 15 mm, and the diameter of the feeding holes 9 is 15 mm.

[0031] As a preferred embodiment of the present invention, an adjusting bolt 8 is provided on the back of the rear side plate 2 , and a strip-shaped adjusting hole is provided at the assembly position of the L-shaped plate 6 corresponding to the rear side plate 2 for adjusting the installation height of the L-shaped plate 6 .

[0032] The experimental steps of the device for observing and recording the mating behavior of fruit flies in the utility model are as follows:

[0033] S01. Assemble the feeding part and the L-shaped plate 6 (without the cover plate), place a piece of cotton paper of the same size on the bottom plate 3 for easy observation, write the number on the feeding plate 4, and place a small piece of thin apple in each feeding hole 9 to provide food for the fruit flies;

[0034] S02. Collect sexually mature male and female fruit flies separately into fruit fly tubes;

[0035] S03. Use CO2 to anesthetize the fruit flies for assembly. First, anesthetize a group (8) of female (male) fruit flies. Use a fine-headed brush to gently brush them one by one and distribute them to different feeding holes 9. Quickly anesthetize a group (8) of male (female) fruit flies. Use the brush to pair them one by one. After each pairing is completed, cover them with a cover plate 5. Push the cover plate 5 in sequence until each group is covered.

[0036] S04. Connect the action camera 7 for shooting to a power source, place it into the observation hole of the L-shaped plate 6, and adjust the installation height of the L-shaped plate 6, the position of the feeding plate 4, and the focal length of the action camera 7 so that each group of feeding holes 9 is in the frame.

[0037] S05. After completing the recording, remove the memory card and connect it to a computer to view the mating video. You can watch it at double speed. The mating start and end times are from the time when the male and female fruit flies begin to show mating posture to when they completely separate.

[0038] Although the above describes the specific implementation methods of the present invention in conjunction with the accompanying drawings, it does not limit the scope of protection of the present invention. Those skilled in the art should understand that on the basis of the technical solution of the present invention, various modifications or variations that can be made by those skilled in the art without creative work are still within the scope of protection of the present invention.

Claims

1. A device for observing and recording the mating behavior of fruit flies, characterized in that: The invention comprises a feeding part, an L-shaped plate and a motion camera. The feeding part comprises a front side plate, a rear side plate, a bottom plate, a feeding plate and a cover plate. The bottom plate is arranged between the front side plate and the rear side plate. The front side plate, the rear side plate and the bottom plate are H-shaped as a whole. Exhaust holes are evenly arranged on the bottom plate. Cotton white paper is placed on the surface of the bottom plate. The feeding plate is placed on the cotton white paper. A plurality of staggered feeding holes are opened on the feeding plate. The cover plate is installed on the feeding plate. The L-shaped plate is installed on the rear side plate. An observation hole is opened on the L-shaped plate. The motion camera is arranged at the observation hole.

2. A device for observing and recording the mating behavior of fruit flies according to claim 1, characterized in that: The back of the rear side plate is provided with an adjusting bolt, and the assembly position of the L-shaped plate corresponding to the rear side plate is provided with an adjusting hole for adjusting the installation height of the L-shaped plate.

3. The device for observing and recording the mating behavior of fruit flies according to claim 1, characterized in that: The bottoms of the front side panels and the rear side panels are both provided with anti-falling stabilizing plates, and the front side panels and the rear side panels are both vertically arranged on the anti-falling stabilizing plates.

4. The device for observing and recording the mating behavior of fruit flies according to claim 3, characterized in that: The feeding part, L-shaped plate and anti-falling stabilizing plate are all made of transparent acrylic.

5. The device for observing and recording the mating behavior of fruit flies according to claim 1, characterized in that: The length of the front side plate and the rear side plate are both 180 mm, the thickness is both 3 mm, the width of the front side plate is 25 mm, and the width of the rear side plate is 40 mm.

6. The device for observing and recording the mating behavior of fruit flies according to claim 1, characterized in that: The length and width of the bottom plate, feeding plate and cover plate are exactly the same, which are 180 mm and 100 mm respectively. The thickness of the bottom plate and cover plate are both 3 mm, and the thickness of the feeding plate is 5 mm.

7. The device for observing and recording the mating behavior of fruit flies according to claim 6, characterized in that: The feeding holes on the feeding plate are arranged in two rows, the two rows of feeding holes are staggered, the row spacing between the two rows of feeding holes is 30 mm, and the spacing between the feeding holes in the same row is 15 mm.

8. The device for observing and recording the mating behavior of fruit flies according to claim 7, characterized in that: The diameter of the feeding hole is 15 mm.

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