Efficient ovum freezing straw
By introducing through holes and observation areas into the oocyte freezing straw, and equipping it with a limiting mechanism and a movable rod, the problem of insufficient oocyte cryopreservation capacity was solved, achieving efficient oocyte freezing and thawing, reducing costs and improving ease of operation.
Patent Information
- Application Number
- CN202422856315.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-22
- Publication Date
- 2025-10-28
- Estimated Expiration
- 2034-11-22
AI Technical Summary
The current storage capacity of frozen egg straws is limited, with each straw only able to store 2-3 eggs, resulting in high freezing and thawing costs and low efficiency.
A high-efficiency oocyte freezing straw was designed. By setting through holes and a transparent observation area in the loading section, the freezing and thawing fluids can be directly introduced. It is also equipped with a limiting mechanism and a movable rod, which facilitates the storage and handling of oocytes and improves loading capacity and operational efficiency.
It improves the efficiency of oocyte freezing and thawing, reduces freezing and thawing costs, increases oocyte loading capacity, simplifies the operation process, and reduces the risk of misoperation.
Smart Images
Figure CN223472933U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the medical field, and in particular to a highly efficient oocyte freezing straw. Background Technology
[0002] In the process of assisted reproductive technology (ART), various in vitro operations are required on human gametes or embryos. For example, if a patient has extra usable embryos or sperm cannot be obtained on the day of egg retrieval, the embryos or eggs must first be vitrified and then stored long-term in liquid nitrogen tanks using cryopreservation straws. Cryopreservation straws are mainly used for the cryopreservation of biological samples. They are made of biocompatible materials and are primarily used in assisted reproductive medicine, especially for the cryopreservation of eggs and embryos. They have advantages such as good sealing and suitability for liquid nitrogen storage. Because controlled ovulation induction techniques are used clinically, each egg retrieval typically yields several, a dozen, or even dozens of eggs. Currently, there are no dedicated cryopreservation straws for eggs. The reagents, procedures, and straws used for egg freezing are basically the same as those for embryos. Therefore, each straw can generally only store two eggs, resulting in high costs for egg freezing and thawing (often tens of thousands of yuan more) and very low freezing-thawing efficiency. Utility Model Content
[0003] This utility model relates to a high-efficiency egg freezing straw. Because the freezing and thawing fluids can flow through the through holes, freezing and thawing operations can be carried out quickly and as a whole. This allows the loading section to store multiple eggs at the same time, which not only improves the efficiency of freezing and thawing but also significantly increases the loading capacity, thereby greatly reducing freezing and thawing costs.
[0004] The technical solution of this utility model is as follows:
[0005] A high-efficiency oocyte freezing straw includes a base and a loading part fixedly disposed on the upper end of the base. The upper end of the loading part is open and surrounded on all four sides. A connecting rod is disposed on one side of the base and is fixedly connected to the base. A pair of parallel and opposing sliding grooves are disposed at the top of the loading part. A movable plate is slidably connected in the sliding grooves. The movable plate includes an opening area that runs vertically through and a transparent observation area. A limiting mechanism for fixing the movable plate is disposed on one side of the loading part. At least one side of the loading part is provided with multiple through holes. A movable rod is hinged to the end of the connecting rod away from the loading part.
[0006] The loading section has a cuboid inner cavity, the opening area is equal in size to the open portion of the loading section, and the observation area is slightly larger than the open portion of the loading section. The length of the chute points towards the connecting rod, and the observation area is located on the side of the opening area away from the connecting rod.
[0007] The distance from the connecting rod to the loading section is greater than the length of the opening area.
[0008] The through-hole array is disposed on three sides of the loading part, and the diameter of the through-hole is 20-25 μm.
[0009] The limiting mechanism includes a limiting block embedded inside the loading part, and a spring is provided at the lower end of the limiting block; a limiting post and a cylindrical pressure block are provided at intervals at the upper end of the limiting block, and the projection of the limiting post in the horizontal direction falls on the moving plate, and the moving plate is provided with a limiting groove corresponding to the limiting post; the loading part is provided with a circular hole at the upper end of the pressure block, and the diameter of the circular hole is equal to or slightly smaller than the diameter of the pressure block.
[0010] The connecting rod has a rotating shaft at the end furthest from the loading part, and the movable rod has a shaft hole at the end where it connects to the connecting rod. The diameter of the rotating shaft is slightly larger than that of the shaft hole.
[0011] The height of the connecting rod is lower than that of the moving plate.
[0012] The base is embedded with a counterweight.
[0013] This utility model has the following beneficial effects:
[0014] 1. Compared with the original frozen straw, the diameter of the through hole in this utility model is 20-25um, which allows the cryo-liquid and thawing fluid to pass through. The cryo-liquid and thawing fluid can directly enter the loading part for freezing and thawing, thus improving the freezing and thawing efficiency. This can increase the loading capacity of the loading part, greatly reduce freezing costs, and alleviate the expenses required for assisted reproduction for patients.
[0015] 2. The movable plate of this utility model can move back and forth to achieve dual functions. The opening area provides the necessary clearance for operation, and the observation area can not only seal the loading part, but also make it convenient for the operator to observe the state of the eggs in the loading part.
[0016] 3. The distance between the connecting rod and the loading part of this utility model provides lateral space for the movement of the moving plate, and the height of the connecting rod is lower than that of the moving plate, which makes it convenient for the operator to push the moving plate laterally, and the operation is simple and reliable.
[0017] 4. The limiting mechanism of this utility model can fix the position of the moving plate by limiting the limiting post, and prevent the moving plate from moving during freezing or thawing; the operator can release the limiting post from limiting the moving plate by pressing the pressure block through the round hole, which can prevent accidental contact that causes the moving plate to move.
[0018] 5. The movable rod of this utility model can be bent upward relative to the connecting rod. When it is necessary to move the frozen wheat straw, the movable rod can be lifted upward and the entire frozen wheat straw can be taken out through the movable rod, making it easier to move. When it is necessary to observe under a microscope or put into an aluminum frame, the movable rod can be folded down and level with the connecting rod, which can avoid interference with the microscope or aluminum frame.
[0019] 6. The base of this utility model is equipped with a counterweight, so the straw will not easily shift or flip when the eggs are placed or removed, which can prevent the eggs from slipping and being lost; it is also more stable when observed under a microscope. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of the present invention;
[0021] Figure 2 This is a side view of the loading part of this utility model;
[0022] Figure 3 This is a top view of the movable plate of this utility model;
[0023] Figure 4 This is a partial cross-sectional view showing the location of the limiting mechanism of this utility model;
[0024] Figure 5 This is a partial cross-sectional view of the location of the rotating shaft of this utility model.
[0025] The reference numerals in the figure are as follows:
[0026] 1-Base, 2-Loading part, 3-Connecting rod, 4-Slide groove, 5-Moving plate, 501-Opening area, 502-Observation area, 6-Through hole, 7-Moving rod, 8-Limiting block, 9-Spring, 10-Limiting post, 11-Pressure block, 12-Round hole, 13-Rotating shaft, 14-Limiting groove. Detailed Implementation
[0027] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.
[0028] See Figures 1 to 5A high-efficiency oocyte freezing straw includes a base 1 and a loading part 2 fixedly disposed on the upper end of the base 1. The upper end of the loading part 2 is open and surrounded on all four sides. The inner cavity of the loading part 2 is preferably a cuboid with a length of 5-6 mm, a width of 3-4 mm, and a height of 3-4 mm. A connecting rod 3 is provided on one side of the base 1, and the connecting rod 3 is fixedly connected to the base 1. The height of the connecting rod 3 is lower than that of the moving plate 5, which facilitates the operator to push the moving plate 5 laterally. A pair of parallel and opposing sliding grooves 4 are provided at the top of the loading part 2. A movable plate 5 is slidably connected inside the chute 4; a limiting mechanism for fixing the movable plate 5 is provided on one side of the loading part 2; multiple through holes 6 are provided on the three sides of the loading part 2, and the diameter of the through holes 6 is preferably 20-25 μm. This size allows the freezing liquid and thawing liquid to pass through, and the freezing liquid and thawing liquid can directly enter the loading part 2 for freezing and thawing, thereby improving the freezing and thawing efficiency and increasing the loading capacity of eggs to about 10-15; a movable rod 7 is hinged to the end of the connecting rod 3 away from the loading part 2.
[0029] Further, see Figure 3 The movable plate 5 includes a through-hole opening 501 and a transparent observation area 502. The size of the opening 501 is equal to the opening of the loading section 2, facilitating operations within the loading section 2. The size of the observation area 502 is slightly larger than the opening of the loading section 2, ensuring that all areas within the loading section 2 can be seen through the observation area 502. The movable plate 5 can move back and forth along the slide 4, achieving a dual function: when the observation area 502 moves to the upper end of the loading section 2, the movable plate 5 closes the loading section 2, preventing the eggs from falling out; additionally, when microscopic observation is required, it can be observed through the transparent observation area 502. The loading section 2 measures the eggs inside; when eggs need to be retrieved or placed, the opening area 501 is moved to the upper end of the loading section 2, and the opening area 501 provides the necessary clearance for operation; the length direction of the slide 4 points to the connecting rod 3, and the observation area 502 is located on the side of the opening area 501 away from the connecting rod 3. When the frozen wheat tube needs to be moved, the observation area 502 needs to be located at the upper end of the loading section 2 to close the loading section 2. At this time, the opening area 501 is located inside the frozen wheat tube, which can reduce the space occupied by the frozen wheat tube; the distance from the connecting rod 3 to the loading section 2 is greater than the length of the opening area 501, providing lateral space for the movement of the moving plate 5.
[0030] Further, see Figure 4The limiting mechanism includes a limiting block 8 embedded inside the loading part 2. A spring 9 is provided at the lower end of the limiting block 8. After the pressure on the limiting mechanism is released, the spring 9 can provide an upward restoring force for the limiting mechanism. A limiting post 10 and a cylindrical pressure block 11 are provided at intervals at the upper end of the limiting block 8. The projection of the limiting post 10 in the horizontal direction falls on the moving plate 5. On the side corresponding to the limiting mechanism, a limiting groove 14 is provided at the middle edge of the opening area 501 and the middle edge of the observation area 502, respectively. The radius of the limiting groove 14 is equal to the radius of the limiting post 10. A circular hole 12 is provided at the upper end of the pressure block 11 in the loading part 2. The diameter of the circular hole 12 is equal to or slightly smaller than the diameter of the pressure block 11. The operator can release the limiting post 10 from limiting the moving plate 5 by pressing the pressure block 11 through the circular hole 12, which can prevent accidental contact that could cause the moving plate 5 to misalign.
[0031] Furthermore, the movable rod 7 can be bent upward relative to the connecting rod 3. When it is necessary to move the frozen straw, the movable rod 7 can be lifted upward, and the entire frozen straw can be taken out through the movable rod 7, making it easier to take out. When it is necessary to observe under a microscope or place it in an aluminum frame, the movable rod 7 can be folded down and level with the connecting rod 3, which can avoid interference with the microscope or aluminum frame. A rotating shaft 13 is provided at the end of the connecting rod 3 away from the loading part 2. The movable rod 7 is provided with a shaft hole at the end where it connects to the connecting rod 3. The diameter of the rotating shaft 13 is slightly larger than the shaft hole. The interference fit between the movable rod 7 and the connecting rod 3 can prevent the movable rod 7 from swinging or misaligning uncontrollably.
[0032] Furthermore, the base 1 is embedded with a counterweight, which can lower the center of gravity of the entire frozen straw. When the eggs are retrieved or placed, the straw will not easily shift or flip, preventing the eggs from slipping and being lost. Under a microscope, the frozen straw is also placed more stably.
[0033] The working principle of this utility model:
[0034] First, press the pressure block 11 inside the circular hole 12 to move the limiting block 8 downwards, thereby releasing the limiting post 10 from limiting the moving plate 5. Then, move the observation area 502 of the moving plate 5 away from the loading section 2, so that the opening area 501 is above the loading section 2. Use a Parvati glass pipette to transfer the eggs into the loading section 2 and fill the loading section 2 with culture medium. Then, press the pressure block 11 inside the circular hole 12 and push the observation area 502 back above the loading section 2 to close the loading section 2. At this time, the loading can be confirmed through the transparent observation area 502. The number of eggs in section 2 should be determined, and the movable rod 7 should be lowered and aligned with the connecting rod 3 to avoid interference with the microscope. Then, the freezing operation should be performed. At this time, the movable rod 7 should be flipped up to the vertical position for easy manual handling. First, gently shake the loading section 2 to allow the culture medium inside to flow out. Then, immerse the loading section 2 in the freezing solution and move it to fill the loading section 2 with the freezing solution. After freezing, drain the freezing solution from the loading section 2, then lower the movable rod 7 and align it with the connecting rod 3. Then, place the frozen straw on the aluminum frame and finally put it into the liquid nitrogen tank for storage.
[0035] When thawing is required, first remove the aluminum frame from the liquid nitrogen tank and remove the frozen wheat tube from the aluminum frame; flip the movable rod 7 upwards to the vertical direction, hold the movable rod 7 and move the loading part 2 in the thawing solution to allow the thawing solution to enter the loading part 2, and the eggs in the loading part 2 will be heated and thawed; after thawing is completed, drain the thawing solution from the loading part 2, and then move the frozen wheat tube to the microscope. At this time, the movable rod 7 will move down and be level with the connecting rod 3. Confirm the number of eggs under the microscope; after thawing is completed, immerse the loading part 2 in the culture medium, press the pressure block 11, and push the opening area 501 above the loading part 2, and then use a Parshall glass pipette to aspirate the eggs for use.
[0036] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.
Claims
1. A highly efficient oocyte freezing straw, characterized in that: The device includes a base (1) and a loading part (2) fixedly disposed on the upper end of the base (1). The upper end of the loading part (2) is open and surrounded on all four sides. A connecting rod (3) is provided on one side of the base (1) and the connecting rod (3) is fixedly connected to the base (1). A pair of parallel and opposing sliding grooves (4) are provided at the top of the loading part (2). A movable plate (5) is slidably connected in the sliding grooves (4). The movable plate (5) includes an opening area (501) that runs vertically through the device and a transparent observation area (502). A limiting mechanism for fixing the movable plate (5) is provided on one side of the loading part (2). At least one side of the loading part (2) is provided with multiple through holes (6). A movable rod (7) is hinged to the end of the connecting rod (3) away from the loading part (2).
2. The efficient oocyte freezing straw as described in claim 1, characterized in that: The inner cavity of the loading part (2) is a cuboid, the size of the opening area (501) is equal to the opening of the loading part (2), and the size of the observation area (502) is slightly larger than the opening of the loading part (2); the length direction of the slide (4) points to the connecting rod (3), and the observation area (502) is located on the side of the opening area (501) away from the connecting rod (3).
3. The high-efficiency oocyte freezing straw as described in claim 2, characterized in that: The distance from the connecting rod (3) to the loading part (2) is greater than the length of the opening area (501).
4. The efficient oocyte freezing straw as described in claim 2, characterized in that: The through holes (6) array is disposed on three sides of the loading part (2), and the diameter of the through holes (6) is 20-25 μm.
5. The efficient oocyte freezing straw as described in claim 1, characterized in that: The limiting mechanism includes a limiting block (8) embedded inside the loading part (2), and a spring (9) is provided at the lower end of the limiting block (8); a limiting post (10) and a cylindrical pressure block (11) are provided at intervals at the upper end of the limiting block (8), and the projection of the limiting post (10) in the horizontal direction falls on the moving plate (5), and the moving plate (5) is provided with a limiting groove (14) corresponding to the limiting post (10); the loading part (2) is provided with a round hole (12) at the upper end of the pressure block (11), and the diameter of the round hole (12) is equal to or slightly smaller than the diameter of the pressure block (11).
6. The efficient oocyte freezing straw as described in claim 1, characterized in that: A rotating shaft (13) is provided at the end of the connecting rod (3) away from the loading part (2), and the movable rod (7) is provided with a shaft hole at the end where it is connected to the connecting rod (3). The diameter of the rotating shaft (13) is slightly larger than that of the shaft hole.
7. The efficient oocyte freezing straw as described in claim 1, characterized in that: The height of the connecting rod (3) is lower than that of the moving plate (5).
8. The efficient oocyte freezing straw as described in claim 1, characterized in that: The base (1) is embedded with a counterweight.