Assembly for taking cryopreserved cells

By designing the cryopreservation cell assembly and utilizing the combination of forceps and fixing pins, the problem of the cryopreservation tubes being difficult to open at low temperatures was solved, enabling rapid disassembly and sealing, and improving the convenience and efficiency of cell retrieval.

CN223554118UActive Publication Date: 2025-11-18NOXIDARON (TIANJIN) BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202423180994.8
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-12-23
Publication Date
2025-11-18
Estimated Expiration
2034-12-23

AI Technical Summary

Technical Problem

In existing technologies, cryovials are at a low temperature after being removed from liquid nitrogen, making it difficult to quickly open the cryovial cap and affecting cell retrieval efficiency.

Method used

Design a component for taking cryopreserved cells, including cryopreservation tubes, extraction mechanism and opening mechanism, so as to achieve quick disassembly and sealing connection of cryopreservation tubes by means of forceps and fixing pins.

Benefits of technology

This technology enables rapid disassembly of the cryopreservation tube caps under low-temperature conditions, improving the convenience and efficiency of cell retrieval.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to an assembly for taking cryopreserved cells, which aims to solve the technical problems that the current cryopreservation tube is low in temperature and inconvenient to open, and comprises a cryopreservation tube, a connecting ring is integrally formed at the top end of the cryopreservation tube, and a plug is inserted into the top end of the cryopreservation tube; the extraction mechanism is used for extracting the cryopreservation tube, the extraction mechanism comprises tweezers, a connecting groove is formed in the top end of the plug, the fixing pins on the two sides of the tweezers can be inserted into the first fixing grooves in the two sides of the connecting groove in the top end of the plug, and the cryopreservation tube can be driven to be taken out from liquid nitrogen through the tweezers; fixing pins on the two sides of tweezers can be inserted into second fixing grooves in the two sides of a connecting groove, meanwhile, the fixing pins can push out fixing blocks in the second fixing grooves so that the fixing blocks can be contained in limiting grooves, the fixing blocks make contact with fixed connection of the plug and the cryopreservation tube, the tweezers are pulled upwards, and the plug can be pulled out of the top end of the cryopreservation tube; and the cryopreserved cells in the cryopreservation tube can be conveniently and quickly taken by a worker.
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Description

TECHNICAL FIELD

[0001] The utility model relates to the field of cell cryopreservation, in particular to a kind of component of taking and using cryopreserved cell. BACKGROUND

[0002] Cell cryopreservation is a technique that places cells in a low-temperature environment to reduce cell metabolism for long-term storage. By using cryopreservation technology, cells can be stored at-196℃ in liquid nitrogen, allowing cells to temporarily leave the growth state and preserve their cell characteristics. This allows cells to be revived for experiments when needed.

[0003] According to the disclosed patent CN212355043U, a kind of component of taking and using cryopreserved cell, including: cover, it includes connecting body and connecting cavity, connecting body is used to assemble with the cryopreserved tube of placing cell, at least one guide slot is included on connecting cavity, connecting through-hole is set on the cavity wall above guide slot;Sampler, one end is placed into connecting cavity, guided by guide slot, and assembled with connecting through-hole, capture single target cryopreserved tube.

[0004] In the process of realizing the utility model, the inventor found that at least the following problems in the prior art have not been solved. In the above case, after the end of the sampler is placed into the connecting cavity, the first pin shaft is guided by the first guide slot, so that the side wall of the first pin shaft touches the first slot wall and the second slot wall, and then the first pin shaft slides into the first connecting through-hole, so that the sampler and the cover are assembled together. The single target cryopreserved tube can be taken out from the liquid nitrogen. However, during use, after the cryopreserved tube is taken out from the liquid nitrogen, the overall temperature is relatively low, which is not convenient for the worker to unscrew the cover at the top end of the cryopreserved tube. If waiting for it to recover to normal temperature, a lot of time will be wasted, and the cells inside the cryopreserved tube may also be affected. Therefore, a new technical solution needs to be designed to solve the problem. UTILITY MODEL CONTENTS

[0005] The utility model aims at overcoming the shortcomings of the prior art, adapting to the actual needs, and providing a kind of component of taking and using cryopreserved cell to solve the technical problem of current cryopreserved tube temperature being relatively low and being inconvenient to open.

[0006] In order to achieve the purpose of the utility model, the technical scheme that the utility model adopts is: a kind of component for taking frozen cell is designed, including cryopreservation tube, the top of the cryopreservation tube is integrally formed with connecting ring, the top of the cryopreservation tube is inserted with plug;Extraction mechanism: for the extraction of cryopreservation tube, the extraction mechanism includes tweezers, the top of the plug is provided with connecting slot, the two sides of the inner wall of the connecting slot are vertically and symmetrically provided with guide slot, the middle part of the guide slot is horizontally provided with first fixed slot, the two sides of the tweezers symmetrically install fixed pin away from handle end, the fixed pin of the two sides of the tweezers is respectively inserted with the first fixed slot of the two sides of connecting slot;Opening mechanism: for opening cryopreservation tube, the inner wall of the two sides of connecting ring is symmetrically provided with limiting slot, the inside of the limiting slot is slidably connected with limiting block, the fixed block is right trapezoid, the middle part of the limiting block is fixedly connected with the end of the fixed block away from inclined plane, the side of the limiting block away from the fixed block is provided with spring, the bottom end of the guide slot is horizontally and through provided with second fixed slot, the end of the fixed block away from the limiting block is inserted with the second fixed slot, and the fixed pin of the two sides of the tweezers is respectively inserted with the second fixed slot of the two sides of connecting slot.

[0007] Preferably, the end of the spring away from the limiting block is fixedly connected with the inner wall of the limiting slot, and the size of the fixed block and the limiting slot is matched.

[0008] Preferably, the side of the upper surface of the connecting ring is vertically and fixedly installed with positioning pin, and the bottom side of the plug is provided with positioning slot, and the positioning pin is inserted with the positioning slot.

[0009] Preferably, the positioning pin corresponds to a group of fixed blocks, and the positioning slot corresponds to a group of second fixed slots.

[0010] Preferably, the outer side of the lower end of the plug is sleeved with sealing ring, and the size of the plug and the connecting ring is matched.

[0011] Preferably, the two groups of fixed blocks correspond to the two groups of second fixed slots, and the size of the fixed pin and the first fixed slot and the second fixed slot is matched.

[0012] Compared with the prior art, the utility model has the beneficial effects that:

[0013] 1.The fixed pin of the two sides of the tweezers can be inserted into the first fixed slot of the two sides of the connecting slot, the cryopreservation tube can be taken out from liquid nitrogen through the tweezers, the fixed pin of the two sides of the tweezers can be inserted into the second fixed slot of the two sides of the connecting slot, at the same time, the fixed pin can push the fixed block in the second fixed slot into the limiting slot, so that the fixed connection of the plug and the cryopreservation tube is contacted, the plug can be pulled out from the top of the cryopreservation tube by pulling the tweezers upward, and the staff can quickly take the frozen cells in the cryopreservation tube.

[0014] 2. The utility model discloses through the top fixed mounting of connecting ring has the positioning pin, the bottom side of the plug -in is equipped with the corresponding positioning slot, can position the plug -in, make the fixed block of connecting ring both sides with the second fixed slot of the both sides of plug -in alignment, through the fixed block is right angle trapezoid, when the bottom end of plug -in is inserted into the connecting ring, through the spring elasticity, can promote the one end of fixed block and insert into the second fixed slot, the fixed connection of convenient cryopreservation tube and plug -in. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is the top view of the utility model;

[0016] Figure 2 It is the bottom side view of the utility model;

[0017] Figure 3 It is the section view of the utility model;

[0018] Figure 4 It is the A part schematic view of the utility model;

[0019] Figure 5 It is the B part schematic view of the utility model;

[0020] In the drawing: 1, cryopreservation tube;11, connecting ring;12, plug -in;13, sealing ring;14, positioning slot;15, positioning pin;2, connecting groove;21, guide groove;22, first fixed slot;23, tweezers;24, fixed pin;3, limit slot;31, limit block;32, fixed block;33, spring;34, second fixed slot. SPECIFIC EMBODIMENTS

[0021] The utility model is further explained in connection with the drawings and examples:

[0022] A kind of component for taking frozen cell, refer to Figures 1 to 5 , including cryopreservation tube 1, the top of cryopreservation tube 1 integrally formed with connecting ring 11, the top of cryopreservation tube 1 is inserted with plug -in 12, the outer side of plug -in 12 lower end is equipped with sealing ring 13, plug -in 12 and the size of connecting ring 11 cooperate;Extraction mechanism: for the extraction of cryopreservation tube 1, extraction mechanism includes tweezers 23, the top of plug -in 12 is equipped with connecting groove 2, connecting groove 2 inner wall both sides are vertically symmetrical and are equipped with guide groove 21, the middle part of guide groove 21 is horizontally equipped with first fixed slot 22, tweezers 23 two sides symmetrical installation fixed pin 24 away from handle end, fixed pin 24 of tweezers 23 both sides respectively with the first fixed slot 22 of connecting groove 2 both sides insertion, two groups of fixed blocks 32 and two groups of second fixed slots 34 correspond, fixed pin 24 and the size of first fixed slot 22 and second fixed slot 34 cooperate.

[0023] The sealing ring 13 sleeved outside the lower end of the plug 12 can improve the sealing effect of the plug 12 on the cryopreservation tube 1. When it is necessary to take the cryopreservation tube 1 out of the liquid nitrogen, the two sides of the tweezers 23 are pressed, so that the fixing pins 24 on the two sides of the tweezers 23 are inserted into the first fixing grooves 22 on the two sides of the connecting groove 2 along the guide grooves 21 on the two sides of the connecting groove 2, and the cryopreservation tube 1 can be taken out of the liquid nitrogen by the tweezers 23. It should be noted that the two sides of the tweezers 23 can be reset after being pressed, and a reset spring can also be installed on the upper end of the inside of the tweezers 23 according to the use requirement. When the tweezers 23 is pulled up, the top end of the tweezers 23 is pinched and pulled.

[0024] Specifically, referring to Figures 1 to 5 , the opening mechanism: used for opening the cryopreservation tube 1, the inner wall on the two sides of the connecting ring 11 is symmetrically provided with a limiting groove 3, the limiting groove 3 is slidably connected with a limiting block 31, one end of the fixing block 32 away from the inclined surface is fixedly connected with the middle part of the limiting block 31, the side of the limiting block 31 away from the fixing block 32 is provided with a spring 33, one end of the spring 33 away from the limiting block 31 is fixedly connected with the inner wall of the limiting groove 3, the bottom end of the guide groove 21 is horizontally provided with a second fixing groove 34, and one end of the fixing block 32 away from the limiting block 31 is inserted into the second fixing groove 34. The fixing pins 24 on the two sides of the tweezers 23 are respectively inserted into the second fixing grooves 34 on the two sides of the connecting groove 2.

[0025] The two sides of the tweezers 23 are pressed, so that the fixing pins 24 are moved out of the first fixing grooves 22, the fixing pins 24 can be pushed downward to align with the second fixing grooves 34 through the guide grooves 21, the tweezers 23 is loosened, the fixing pins 24 on the two sides of the tweezers 23 can be inserted into the second fixing grooves 34, at the same time, the fixing pins 24 can push the fixing blocks 32 in the second fixing grooves 34 to make them enter the limiting grooves 3, so as to contact the fixed connection between the plug 12 and the cryopreservation tube 1. Pulling the tweezers 23 upward can pull the plug 12 out of the top end of the cryopreservation tube 1, which is convenient for the staff to quickly take the cryopreservation cells in the cryopreservation tube 1.

[0026] Further, referring to Figures 1 to 3 , the upper surface of the connecting ring 11 is vertically fixedly installed with a positioning pin 15, the bottom side of the plug 12 is provided with a positioning groove 14, the positioning pin 15 is inserted into the positioning groove 14, the positioning pin 15 corresponds to one group of fixing blocks 32, and the positioning groove 14 corresponds to one group of second fixing grooves 34.

[0027] The positioning pin 15 is fixedly installed at the top end of the connecting ring 11, the bottom side of the plug 12 is provided with a corresponding positioning groove 14, the plug 12 can be positioned, the fixing blocks 32 on both sides of the connecting ring 11 are aligned with the second fixing grooves 34 on both sides of the plug 12, when the bottom end of the plug 12 is inserted into the inner side of the connecting ring 11 and the fixing blocks 32 are aligned with the second fixing grooves 34, the two sets of limiting blocks 31 can be pushed to move relatively by the elastic force of the spring 33, and one end of the two sets of fixing blocks 32 is further pushed out of the limiting groove 3 and inserted into the corresponding second fixing groove 34, so that the cryopreservation tube 1 and the plug 12 are fixedly connected.

[0028] It is worth mentioning that, referring to Figures 1 to 5 The fixing block 32 is a right-angled trapezoid, and the size of the fixing block 32 is matched with the size of the limiting groove 3.

[0029] The fixing block 32 is a right-angled trapezoid, and the size of the fixing block 32 is matched with the size of the limiting groove 3.

[0030] In addition, the components of the utility model are general standard components or components known by those skilled in the art, the structure and principle of which can be known by the technical personnel through a technical manual or through a conventional experimental method, and the technical personnel in the art can completely realize without redundancy, and the content protected by the utility model does not involve improvement of internal structure and method.

[0031] The utility model discloses an embodiment discloses a preferred embodiment, but is not limited to this, and the ordinary skilled in the art, easily understands the spirit of the utility model according to the above embodiment, and makes different induction and change, but as long as not departing from the spirit of the utility model, is within the protection scope of the utility model.

Claims

1. A component for taking cryopreserved cells, comprising cryovials (1), characterized in that, The top end of the cryopreservation tube (1) is integrally formed with a connecting ring (11), and the top end of the cryopreservation tube (1) is inserted with a plug (12). Extraction mechanism: used for extracting cryopreservation tube (1), the extraction mechanism includes tweezers (23), the top of the plug (12) is provided with a connecting groove (2), the inner wall of the connecting groove (2) is vertically symmetrically provided with guide grooves (21) on both sides, the middle of the guide groove (21) is horizontally provided with a first fixing groove (22), the tweezers (23) is symmetrically provided with fixing pins (24) on both sides away from the handle, and the fixing pins (24) on both sides of the tweezers (23) are respectively inserted into the first fixing grooves (22) on both sides of the connecting groove (2); Opening mechanism: used to open the cryopreservation tube (1). The opening mechanism includes two sets of fixing blocks (32). The inner walls on both sides of the connecting ring (11) are symmetrically provided with limiting grooves (3). The limiting grooves (31) are slidably connected inside the limiting grooves (3). The fixing blocks (32) are right-angled trapezoids. The end of the fixing block (32) away from the inclined surface is fixedly connected to the middle of the limiting block (31). A spring (33) is provided on the side of the limiting block (31) away from the fixing block (32). The bottom end of the guide groove (21) is horizontally provided with a second fixing groove (34). The end of the fixing block (32) away from the limiting block (31) is inserted into the second fixing groove (34). The fixing pins (24) on both sides of the tweezers (23) are respectively inserted into the second fixing grooves (34) on both sides of the connecting groove (2).

2. The assembly for taking cryopreserved cells as described in claim 1, characterized in that, The end of the spring (33) away from the limiting block (31) is fixedly connected to the inner wall of the limiting groove (3), and the size of the fixing block (32) matches that of the limiting groove (3).

3. The component for taking cryopreserved cells as described in claim 1, characterized in that, A positioning pin (15) is vertically fixed on one side of the upper surface of the connecting ring (11), and a positioning groove (14) is provided on the bottom side of the plug (12). The positioning pin (15) is inserted into the positioning groove (14).

4. The component for taking cryopreserved cells as described in claim 3, characterized in that, The positioning pin (15) corresponds to a set of fixing blocks (32), and the positioning groove (14) corresponds to a set of second fixing grooves (34).

5. The assembly for taking cryopreserved cells as described in claim 1, characterized in that, A sealing ring (13) is fitted on the outer side of the lower end of the plug (12), and the size of the plug (12) matches that of the connecting ring (11).

6. The assembly for taking cryopreserved cells as described in claim 1, characterized in that, The two sets of fixing blocks (32) correspond to the two sets of second fixing grooves (34), and the fixing pins (24) are sized to match the first fixing groove (22) and the second fixing groove (34).

Citation Information

Patent Citations

  • Assembly for taking cryopreserved cells

    CN212355043U