Restraining device for depression of adult zebra fish

By designing a zebrafish restraint device with adjustable thickness and color, the problems of difficult zebrafish restraint and visual contact in existing technologies have been solved, achieving safe and efficient experimental conditions, adapting to zebrafish of different sizes and moods, and improving experimental efficiency and accuracy.

CN223627636UActive Publication Date: 2025-12-05YUNNAN UNIVERSITY OF CHINESE MEDICINE
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Patent Information

Application Number
CN202422623168.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-10-29
Publication Date
2025-12-05
Estimated Expiration
2034-10-29

AI Technical Summary

Technical Problem

Existing techniques for using centrifuge tubes to restrain zebrafish present problems such as difficulty in loading, easy injury to zebrafish, inability to flexibly adjust the restraint space and visual contact, resulting in low experimental efficiency and inaccurate results.

Method used

A zebrafish restraint device was designed, comprising a box, restraint cells, limiting cells, and a cover plate. By adjusting the thickness and color of the limiting cells, it can adapt to zebrafish of different sizes and moods, achieving flexible restraint and water flow control.

Benefits of technology

It improves the efficiency and safety of zebrafish restraint, adapts to zebrafish of different sizes and moods, reduces visual contact interference, and saves experimental time.

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Abstract

The utility model discloses an adult zebra fish depression restraining device which comprises a cuboid box body, restraining cells with the same size are arranged in the box body, and the front portion and the rear portion of each restraining cell are respectively provided with a large water outlet hole. A limiting cell is arranged in each binding cell, the length, the width and the height of the limiting cell are smaller than those of the binding cell, and the limiting cell is inserted into the binding cell; the restraint device for the depression of the adult zebra fish further comprises a cover plate, a hinge is arranged at the top of one long edge of the box body, and the cover plate is connected with the box body through the hinge. The utility model provides the binding device for the depression of the zebra fish, which is safe, time-saving and flexible, and has the functions of adjusting the space size and adjusting the vision.
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Description

TECHNICAL FIELD

[0001] The utility model relates to animal restraint device related technical field, especially a kind of restraint device of adult zebra fish depression model. BACKGROUND

[0002] In the field of zoology research, it is often necessary to construct zebra fish models (such as depression models, anxiety models) involving restraint devices. Currently, the commonly used method is to place experimental zebra fish into a microcentrifuge tube with a tube diameter of 16.8 x 100 mm and a capacity of 2 mL for restraint. The centrifuge tube cap is closed, and the two ends are slightly open to allow the water inside the tube to circulate freely. The zebra fish used to construct the model are about 6-9 months old and about 3-5 cm long. The centrifuge tube used is larger in diameter than the body axis of the zebra fish. The centrifuge tube containing the zebra fish is clamped onto a vertical tube stand and placed in a water tank. However, this method has the following disadvantages: the process of loading the zebra fish into the centrifuge tube is difficult and time-consuming, and may cause certain external injuries to the zebra fish; the zebra fish needs to be restrained multiple times during the experiment, which may cause the death of the zebra fish; the top opening of the centrifuge tube is sharp, and larger zebra fish may be injured on the nose, posing a risk of infection and interfering with the experimental results; the microcentrifuge tube is not suitable for larger or smaller zebra fish, and the restraint space cannot be adjusted flexibly; there is visual contact between each centrifuge tube containing zebra fish, which is not suitable for more experimental requirements.

[0003] In view of the above technical problems, the utility model provides a safe, time-saving and flexible zebra fish depression restraint device that can adjust the size of the restraint space and the color vision. UTILITY MODEL CONTENTS

[0004] The utility model aims to provide a zebra fish depression restraint device to solve the problems of low loading efficiency, easy injury to zebra fish and inability to adjust flexibly.

[0005] The technical solution of the utility model is as follows:

[0006] The zebra fish depression restraint device of the utility model comprises a cuboid box body, the box body is provided with restraint unit cells of the same size, each restraint unit cell is provided with a large water outlet hole at the front and back, a limiting unit cell is arranged in each restraint unit cell, the length, width and height of the limiting unit cell are smaller than those of the restraint unit cell, and the limiting unit cell is inserted into the restraint unit cell, and the zebra fish depression restraint device further comprises a cover plate, a hinge is arranged on the top of one long side of the box body, and the cover plate is connected to the box body through the hinge.

[0007] The limiting cell can be directly inserted into the restraint cell, and then the zebrafish is placed into the limiting cell, and water exchange in the box is realized through the large water outlet holes in front and back of the restraint cell.

[0008] Further, the box body is provided with 5 restraint cells, and 5 fish can be experimented at the same time.

[0009] Further, the cover plate is provided with a small water outlet hole, and each restraint cell is provided with a small water outlet hole at the upper portion, and the water exchange in the river is realized by the large water outlet hole and the small water outlet hole on the cover plate.

[0010] Further, the limiting cell is provided with a pullable taking and placing strip, and the taking and placing strip is fixed in the inside of the limiting cell. The experimenter can conveniently drive the taking and placing limiting cell by moving the taking and placing strip.

[0011] Further, the limiting cell is provided with different thicknesses in the range of 2mm-10mm, and different thicknesses of the limiting cell can cause different internal spaces to adapt to zebrafish of different sizes.

[0012] Further, the color of the limiting cell is provided with three types of yellow, black and transparent, and the experimental zebrafish likes or dislikes the three colors, and the mood variable of the experimental zebrafish can be controlled at the same time.

[0013] Further, the box body, the restraint cell, the limiting cell, the cover plate and the taking and placing strip are made of acrylic, and the physical and chemical properties of the acrylic material are stable at normal temperature, and the consumables are easy to obtain and manufacture.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] 1. The restraint device for adult zebrafish depression of the utility model can adjust the thickness of the limiting cell, thereby adjusting the restraint space of the experimental zebrafish, and adapting to experimental zebrafish of different sizes.

[0016] 2. The utility model can control the mood variable of the experimental zebrafish through the color of the limiting cell.

[0017] 3. The utility model can simultaneously experiment and observe multiple experimental bodies under the condition that the water flow variable is the same, thereby saving the time cost of the experiment. BRIEF DESCRIPTION OF DRAWINGS

[0018] Fig. 1 It is a schematic view of the restraint device for adult zebrafish depression of the utility model.

[0019] Fig. 2 It is a top view of the utility model.

[0020] Fig. 1 - box, 11 - hinge, 2 - restraint unit cell, 3 - large water outlet hole, 4 - limiting unit cell, 5 - cover plate, 6 - small water outlet hole, 7 - taking and placing strip. DETAILED DESCRIPTION

[0021] It should be noted that the relational terms herein, such as first and second, and the like, are used solely to distinguish one from another entity or action without necessarily requiring or implying any actual relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. An element proceeded by "comprises... a" does not, without more constraints, exclude the existence of additional identical elements in the process, method, article, or apparatus that comprises the element.

[0022] The utility model relates to a restraint device for adult zebra fish depression, including the box 1 of cuboid, be provided with same size restraint unit cell 2 in the box 1, every restraint unit cell 2 is provided with one large water outlet hole 3 in front and back, be provided with limiting unit cell 4 in every restraint unit cell 2, the length, width and height of limiting unit cell 4 are less than the length, width and height of restraint unit cell 2, and limiting unit cell 4 is inserted into restraint unit cell, the restraint device for adult zebra fish depression still includes the cover plate 5, is provided with the hinge 11 in the top of one of the long side of box, and the cover plate 5 is connected with the box 1 through the hinge 11.

[0023] The features and performance of the utility model will be further described in detail below in conjunction with examples.

[0024] Example one

[0025] Please refer to Figs. 1-2 When using the restraint device for adult zebra fish depression, first determine the size of experimental zebra fish and experimental quantitative and variable, select the limiting unit cell 4 of corresponding thickness, and the experimental personnel hand the taking and placing strip 7 of selected limiting unit cell 4, and the selected limiting unit cell 4 is inserted into restraint unit cell 2.

[0026] Then fill the water in the box 1, and the corresponding experimental zebra fish is placed in the corresponding limiting unit cell 4 with fishing net, and the cover plate 5 is covered, and finally the whole box 1 is placed in the fish tank of flowing water.

[0027] The thickness and color of the limiting cell 5 can be replaced according to experimental requirements, wherein the yellow limiting cell 4 is the most hated color of zebrafish, and can enhance the effect of restraint stress; the black limiting cell 4 can block the visual contact between each fish; and the transparent limiting cell 4 only provides restraint stress.

[0028] The above-described embodiments only express the specific implementation of the present application, which is described in detail, but cannot be understood as a limitation on the protection scope of the present application. It should be noted that, for ordinary skilled persons in the art, without departing from the technical concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application.

Claims

1. A restraint device for depression of adult zebrafish comprising a cuboid box body (1), characterized in that, The box body (1) is provided with the same size restraint cell (2), each restraint cell (2) is provided with a large water outlet hole (3) in front and back; each restraint cell (2) is provided with a limiting cell (4), the length, width and height of the limiting cell (4) are smaller than the length, width and height of the restraint cell (2), and the limiting cell (4) is inserted into the restraint cell (2); the restraint device for adult zebra fish depression also comprises a cover plate (5), a hinge (11) is arranged on the top of one of the long edges of the box body (1), and the cover plate (5) is connected with the box body (1) through the hinge (11).

2. The restraint device for adult zebrafish depression according to claim 1, wherein The box body (1) is provided with five restraint cells (2).

3. The restraint device for adult zebrafish depression according to claim 1 or 2, wherein, The cover plate (5) is provided with a small water outlet hole (6), and each restraint cell (2) is provided with a small water outlet hole (6) on the upper part.

4. The restraint device for adult zebrafish depression according to claim 1, wherein, The limiting cell (4) is provided with a pullable taking and placing strip (7), the taking and placing strip (7) is fixed to the inner side of the limiting cell (4), and the experimenters can conveniently move the taking and placing strip (7) to drive the taking and placing limiting cell (4).

5. The restraint device for adult zebrafish depression of claim 1, wherein, The limiting cell (4) is provided with different thicknesses in the range of 2mm-10mm.

6. The restraint device for adult zebrafish depression according to claim 1 or 5, wherein, The color of the limiting cell (4) is provided with three types of yellow, black and transparent.

7. The restraint device for adult zebrafish depression of claim 1, wherein, The materials of the box body (1), the restraint cell (2), the limiting cell (4), the cover plate (5) and the taking and placing strip (7) are acrylic.