Main control circuit system for FISH equipment

Abstract

The utility model relates to a main control circuit system for FISH (fluorescence in situ hybridization) equipment, which belongs to the technical field of control circuit systems and comprises a main control board, a temperature control board, a reagent input control board and a diaphragm pump module electrically connected with the main control board. The temperature control board is used for adjusting the temperature of the reaction tank after receiving the preset temperature and the temperature control duration sent by the main control board and feeding back the real-time temperature of the reaction tank to the main control board; the reagent input control board is used for exchanging data with the main control board and feeding back a speed regulation signal to the diaphragm pump module through the main control board; the diaphragm pump module is used for receiving the speed regulation signal and starting or stopping pumping the reagent into or discharging the reagent out of the reaction tank. According to the utility model, the technical problem that hidden dangers exist in line connection in the main control circuit system due to complex system wiring in the prior art is effectively solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to control circuit system technical field especially relates to a main control circuit system for FISH equipment. BACKGROUND

[0002] FISH (fluorescence in situ hybridization) pretreatment equipment is mainly for sample processing instrument before pathological analysis of cytology sample, histology sample (puncture or neutral formalin fixed paraffin-embedded tissue section sample) and blood sample, realizes the pretreatment before FISH denaturation hybridization and the cleaning process after hybridization. During the working process of FISH equipment, the following operations are often needed: diaphragm pump driving and control, magnetic stirring motor control, serial screen display and control, bluetooth serial communication (data feedback), serial debugging data, temperature control, reagent input valve control, sensor signal reading, diaphragm pump current signal sampling and feedback, waste liquid valve driving and control, three-way valve control and other operations.

[0003] It can be seen that the peripherals connected by the system and the control tasks realized are complex, and the control circuit board often needs a large volume in the face of so many controls, and there are a large number of external connection terminals on the control circuit board, which easily leads to complex system wiring and a large number of unstable factors in the production and debugging stages of the equipment. UTILITY MODEL CONTENT

[0004] Therefore, it is necessary to provide a main control circuit system for FISH equipment to solve the technical problem that the internal line connection of the main control circuit system has hidden dangers due to the complex system wiring in the prior art.

[0005] To solve the above problems, the utility model provides a main control circuit system for FISH equipment, comprising: a main control board, a temperature control board and a reagent input control board in communication connection with the main control board, and a diaphragm pump module in electrical connection with the main control board.

[0006] The temperature control board is used for temperature adjustment of the reaction tank and feedback of the real-time temperature of the reaction tank to the main control board after receiving the preset temperature and temperature control duration sent by the main control board.

[0007] The reagent input control board is used for data exchange with the main control board and feedback of the speed regulation signal to the diaphragm pump module through the main control board.

[0008] The diaphragm pump module is used for receiving the speed regulation signal and starting or stopping pumping or discharging the reagent into or out of the reaction tank.

[0009] In a possible implementation manner, the diaphragm pump module comprises: two-way diaphragm pumps for pumping or discharging the reagent into or out of the reaction tank, a diaphragm pump control module and a diaphragm pump sampling module in electrical connection with the diaphragm pump and the main control board.

[0010] The diaphragm pump control module is used for receiving a speed regulation signal transmitted by the main control board to control one of the diaphragm pumps to pump reagents into a reaction tank or discharge reagents from the reaction tank.

[0011] The diaphragm pump sampling module is used for sampling working currents of the two diaphragm pumps.

[0012] In a possible implementation, the diaphragm pump control module comprises two diaphragm pump interfaces, one end of each of which is electrically connected to one of the two diaphragm pumps, and the other end of each of which is electrically connected to the main control board through a signal buffer.

[0013] In a possible implementation, each of the two diaphragm pump interfaces comprises a first pin, a second pin and a third pin.

[0014] The first pin is used for receiving the speed regulation signal, the second pin is electrically connected to the diaphragm pump sampling module, and the third pin is electrically connected to a positive electrode of an external 24V power supply.

[0015] The diaphragm pump sampling module comprises a first sampling circuit and a second sampling circuit.

[0016] The first sampling circuit comprises a first resistor, a second resistor, a current amplifier and a first capacitor.

[0017] The current amplifier comprises a positive input end, a negative input end, an output end, a positive power supply electrode, a negative power supply electrode and a reference voltage pin.

[0018] One end of the first resistor is electrically connected to the positive input end of the current amplifier and the second pin of one of the diaphragm pump interfaces, respectively, and the other end thereof is electrically connected to the negative input end of the current amplifier and grounded, and is used for sampling the working current of the diaphragm pump.

[0019] The output end of the current amplifier is grounded through the second resistor, the positive power supply electrode is electrically connected to an external power supply, the negative power supply electrode is short-circuited to the reference voltage pin and then grounded, and is electrically connected to the external power supply through the first capacitor.

[0020] The second sampling circuit comprises an operational amplifier, a third resistor, a fourth resistor, a fifth resistor and a second capacitor.

[0021] The positive input end of the operational amplifier is electrically connected to the second pin of the other diaphragm pump interface, the negative input end thereof is electrically connected to one end of the fourth resistor and the fifth resistor, respectively, and the output end thereof is electrically connected to the main control board through the third resistor.

[0022] The end of the third resistor electrically connected to the main control board is also grounded through the second capacitor.

[0023] The other end of the fourth resistor is electrically connected with an output end of the operational amplifier, and the other end of the fifth resistor is grounded.

[0024] In a possible implementation, the system further comprises a liquid detection module, which is in communication connection with the reagent input control panel through the main control panel.

[0025] The liquid detection module comprises a T-shaped sensor signal acquisition module for sending a first detection signal when there is liquid in the inlet and outlet pipeline of the reaction tank and a liquid level sensor signal acquisition module for sending a second detection signal when liquid in the reaction tank reaches a preset liquid level.

[0026] The T-shaped sensor signal acquisition module and the liquid level sensor signal acquisition module are independently arranged.

[0027] The T-shaped sensor signal acquisition module comprises two optical liquid level switches arranged on the inlet and outlet pipelines of the reaction tank.

[0028] The liquid level sensor signal acquisition module comprises an optical conical liquid level sensor arranged at the preset liquid level in the reaction tank.

[0029] In a possible implementation, the T-shaped sensor signal acquisition module is electrically connected with the main control panel through a first control circuit.

[0030] The liquid level sensor signal acquisition module is electrically connected with the main control panel through a second control circuit.

[0031] The reagent input control panel is further configured to send a turn-on or turn-off signal to the first control circuit and / or the second control circuit through the main control panel, so as to start or stop the T-shaped sensor signal acquisition module and / or the liquid level sensor signal acquisition module.

[0032] In a possible implementation, the system further comprises a magnetic stirring module, which comprises a magnetic stirrer for stirring and mixing reagents and a motor interface electrically connected with the magnetic stirrer.

[0033] One end of the motor interface is electrically connected with the magnetic stirrer, and the other end is electrically connected with the main control panel.

[0034] The main control panel is further configured to send a start signal to the magnetic stirrer through the motor interface after receiving a real-time temperature, so as to control the magnetic stirrer to start stirring and mixing reagents.

[0035] In a possible implementation, the system further comprises a power conversion module, which is electrically connected with an external 24V power supply, the main control panel, the temperature control panel, the reagent input control panel, the diaphragm pump module, the liquid detection module and the magnetic stirring module respectively, and is configured to supply power.

[0036] In a possible implementation, the power conversion module comprises: a 24V / 12V converter, a 12V / 5V converter and a 5V / 3.3V converter.

[0037] In a possible implementation, the system further comprises:

[0038] A serial port screen communication circuit is in communication connection with the main control board and an external capacitor serial port screen, and is configured to realize data exchange of the main control board and the external capacitor serial port screen.

[0039] A debugging serial port is configured to debug the main control circuit system.

[0040] A Bluetooth communication circuit is in communication connection with the main control board and an external Bluetooth device, and is configured to transmit working states of various modules and modules in the main control circuit system to the external Bluetooth device during debugging.

[0041] An RS485 communication circuit comprises an SP3485 chip and is in communication connection with the main control board, the temperature control board and the reagent input control board, and is configured to realize communication between the main control board and the temperature control board and the reagent input control board.

[0042] The main control circuit system for the FISH equipment provided by the utility model has the advantages that: the main control circuit system for the FISH equipment provided by the utility model comprises multiple control boards including a main control board, a temperature control board and a reagent input control board, data exchange is realized through communication connection between the control boards, signal acquisition and control are implemented on the control boards, wire connection between the control boards, sensors and pumps / valves can be greatly reduced, the system wiring complexity is reduced and stability is improved when the main control board completes overall system coordination, and the technical problem that the line connection in the main control circuit system has hidden dangers due to complex system wiring in the prior art is effectively solved. BRIEF DESCRIPTION OF DRAWINGS

[0043] Figure 1 A structure schematic view of an embodiment of the main control circuit system for the FISH equipment provided by the utility model is provided.

[0044] Figure 2 A structure schematic view of an embodiment of a diaphragm pump interface and a first sampling circuit in the isolation module provided by the utility model is provided.

[0045] Figure 3 A structure schematic view of an embodiment of another diaphragm pump interface and a second sampling circuit in the isolation module provided by the utility model is provided. DETAILED DESCRIPTION

[0046] It should be understood that the specific embodiments described herein are merely exemplary and are not intended to limit the present application.

[0047] The technical solutions in the embodiments of the present application will be clearly and completely described with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0048] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present application are only used to explain the relative positional relationship, movement condition, etc. between components in a certain posture (as shown in the drawings), and if the certain posture changes, the directional indications will also change accordingly.

[0049] In addition, the description of "first", "second" and the like in the present application is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of ordinary skilled in the art, when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, and is not within the scope of protection required by the present application.

[0050] In this document, reference to "embodiment" means that the specific feature, structure or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The phrase appears at various places in the specification does not necessarily all refer to the same embodiment, nor is it necessarily independent or alternative embodiments to other embodiments. Those skilled in the art explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0051] As Figure 1 shown, one specific embodiment of the present application discloses a main control circuit system 10 for FISH equipment, comprising:

[0052] The main control board 110, the temperature control board 120 and the reagent input control board 130 in communication connection with the main control board, and the diaphragm pump module 140 in electrical connection with the main control board;

[0053] Optionally, the main control board 110 can adopt STM32F013ZET6, and the main control board 110 and the temperature control board 120 and the reagent input control board 130 can adopt RS485 communication.

[0054] The temperature control board 120 is used for temperature adjustment of the reaction tank after receiving the preset temperature and temperature control duration sent by the master control board 110 and feeding back the real-time temperature of the reaction tank to the master control board 110.

[0055] The reagent input control board 130 is used for data exchange with the master control board 110 and feeding back a speed regulation signal to the diaphragm pump module 140 through the master control board 110.

[0056] The diaphragm pump module 140 is used for receiving the speed regulation signal and starting or stopping pumping or discharging the reagent into or out of the reaction tank.

[0057] Compared with the prior art, the utility model provides a master control circuit system for a FISH equipment, which is divided into multiple control boards including a master control board, a temperature control board and a reagent input control board, data exchange is carried out between the control boards through communication connection, and signal acquisition and control are implemented by the control boards in proximity, which can greatly reduce wire connection between the boards, the boards-sensors and the boards-pumps / valves, so that the master control board can complete overall system coordination, reduce system wiring complexity and improve stability, thereby effectively solving the technical problem of hidden dangers in internal line connection of the master control circuit system caused by complex system wiring in the prior art.

[0058] In a possible implementation manner, the diaphragm pump module 140 comprises: two diaphragm pumps 1410 and 1420 respectively used for pumping or discharging the reagent into or out of the reaction tank, a diaphragm pump control module 1430 electrically connected with the diaphragm pumps 1410 and 1420 and the master control board 110, and a diaphragm pump sampling module 1440.

[0059] The diaphragm pump control module 1430 is used for receiving the speed regulation signal transmitted by the master control board 110 to control the diaphragm pump 1410 or 1420 to pump the reagent into the reaction tank or discharge the reagent out of the reaction tank.

[0060] The diaphragm pump sampling module 1440 is used for sampling working currents of the two diaphragm pumps 1410 and 1420.

[0061] In a possible implementation manner, the diaphragm pump control module 1430 comprises: two diaphragm pump interfaces CN4 and CN5, one end of each of which is electrically connected with the two diaphragm pumps 1410 and 1420, and the other end of each of which is electrically connected with the master control board 110 through a signal buffer 1450.

[0062] In a possible implementation manner, the two diaphragm pump interfaces CN4 and CN5 each comprise a first pin, a second pin and a third pin.

[0063] The first pin is used for receiving a speed regulation signal BMP1 and BMP2, the second pin is electrically connected with the diaphragm pump sampling module 1440, and the third pin is electrically connected with a positive electrode of an external 24V power supply.

[0064] The diaphragm pump sampling module 1440 comprises a first sampling circuit 14401 and a second sampling circuit 14402.

[0065] As Figure 2 The first sampling circuit 14401 comprises a first resistor R88, a second resistor R129, a current amplifier U34 and a first capacitor C35.

[0066] The current amplifier U34 comprises a positive input end IN+, a negative input end IN-, an output end OUT, a power supply positive electrode V+, a power supply negative electrode GND and a reference voltage pin REF.

[0067] One end of the first resistor R88 is electrically connected with the positive input end IN+ of the current amplifier U34 and a second pin of one of the diaphragm pump interfaces CN4 respectively, and the other end is electrically connected with the negative input end IN- of the current amplifier U34 and grounded, for sampling a working current of the diaphragm pump 1410.

[0068] The output end OUT of the current amplifier U34 is grounded through the second resistor R129, the power supply positive electrode V+ is electrically connected with an external power supply VCC, and the power supply negative electrode GND is short-circuited with the reference voltage pin REF and grounded and electrically connected with the external power supply VCC through the first capacitor C35.

[0069] Optionally, two ADC interfaces ADC1 and ADC2 can be equipped on the main control board 110, and the output signals of the first sampling circuit and the second sampling circuit are received through the two ADC interfaces. R129 is a 0.1 ohm alloy sampling resistor, U34 is a high-precision current amplifier that can be powered by a single power supply, for amplifying the voltage across R129 and sending it to the main control board for AD collection. When used, R88 and C36 need to be used together. In the design, a 100V / V amplification ratio (100 times) is selected. In this circuit, the total ground current of the diaphragm pump is mainly collected, so the current and power consumption of the diaphragm pump can be accurately known, and the corresponding working state can be distinguished.

[0070] As Figure 3 The second sampling circuit 14402 comprises an operational amplifier U5.1, a third resistor R23, a fourth resistor R25, a fifth resistor R26 and a second capacitor CD4.

[0071] The positive input end of the operational amplifier U5.1 is electrically connected with the second pin of another diaphragm pump interface CN5, the negative input end is electrically connected with one end of the fourth resistor R25 and the fifth resistor R26 respectively, and the output end is electrically connected with the main control panel 110 through the third resistor R23;

[0072] One end of the third resistor R23 electrically connected with the main control panel 110 is also grounded through the second capacitor CD4;

[0073] The other end of the fourth resistor R25 is electrically connected with the output end of the operational amplifier U5.1, and the other end of the fifth resistor R26 is grounded.

[0074] Optionally, R14 is a 0.05 ohm alloy resistor, mainly used for collecting the real-time current current1 of the diaphragm pump. After the feedback voltage is amplified by the in-phase proportional amplifier composed of U, R26, R25, R23 and C31, it is sent to the ADC1 sampling interface of the main control panel. The circuit voltage can be amplified by 100 times, and at the same time, it is processed by the low-pass filter circuit composed of R23 and C31.

[0075] It should be noted that the control and current sampling of the diaphragm pump are realized based on the two current amplification and signal processing circuits, and the precision and flexibility of the separated amplification and the integrated special IC amplification can be effectively compared, so that the debugging of the related output circuit is better. Among them, Figure 3 The separated circuit shown is more suitable for adjusting the amplification ratio coefficient, and is more flexible and calibrated.

[0076] In a possible implementation, the system 10 further includes a liquid detection module 150, which is in communication connection with the reagent input control panel 130 through the main control panel 110;

[0077] The liquid detection module 150 includes a T-shaped sensor signal acquisition module 1510 for sending a first detection signal to the reagent when there is liquid in the inlet and outlet pipelines of the reaction tank, and a liquid level sensor signal acquisition module 1520 for sending a second detection signal when the liquid in the reaction tank reaches a preset liquid level;

[0078] The T-shaped sensor signal acquisition module 1510 and the liquid level sensor signal acquisition module 1520 are independently arranged;

[0079] The T-shaped sensor signal acquisition module 1510 includes two photoelectric liquid level switches arranged on the inlet and outlet pipelines of the reaction tank;

[0080] The liquid level sensor signal acquisition module 1520 includes a photoelectric conical liquid level sensor arranged at the preset liquid level in the reaction tank.

[0081] In a possible implementation, the T-shaped sensor signal acquisition module 1510 is electrically connected to the main control board 110 through the first control circuit 1530.

[0082] The liquid level sensor signal acquisition module 1520 is electrically connected to the main control board 110 through the second control circuit 1540.

[0083] The reagent input control board 130 is further configured to send a turn-on or turn-off signal to the first control circuit 1530 and / or the second control circuit 1540 through the main control board 110, so as to start or stop the T-shaped sensor signal acquisition module 1510 and / or the liquid level sensor signal acquisition module 1520.

[0084] In a possible implementation, the system further includes a magnetic stirring module 160, which includes a magnetic stirrer 1610 for stirring and mixing reagents and a motor interface 1620 electrically connected to the magnetic stirrer.

[0085] One end of the motor interface 1620 is electrically connected to the magnetic stirrer 1610, and the other end is electrically connected to the main control board 110.

[0086] The main control board 110 is further configured to send a start signal to the magnetic stirrer 1610 through the motor interface after the temperature control board 120 receives the real-time temperature, so as to control the magnetic stirrer 1610 to start stirring and mixing reagents.

[0087] It should be noted that the magnetic stirring module is mainly brushless motor driving and control, which is used to implement liquid stirring and mixing when the temperature in the reaction tank is rising or falling. Through this technical means, the uniform distribution of temperature can be greatly promoted, and the overall effect of FISH pretreatment can be improved.

[0088] In a possible implementation, the system further includes a power conversion module 170, which is electrically connected to the external 24V power supply 20, the main control board 110, the temperature control board 120, the reagent input control board 130, the diaphragm pump module 140, the liquid detection module 150, and the magnetic stirring module 160, respectively, and is used for power supply.

[0089] In a possible implementation, the power conversion module 170 includes a 24V / 12V converter, a 12V / 5V converter, and a 5V / 3.3V converter.

[0090] It can be understood that the power conversion module is mainly for converting the 24V power supply input by the system into +12V, +5V, and +3.3V, so as to provide appropriate power supply for the main control board and other circuits.

[0091] In a possible implementation, the system 10 further includes:

[0092] The serial screen communication circuit 180 is in communication connection with the main control board 110 and the external capacitor serial screen 30, and is used for realizing data exchange of the main control board 110 and the external capacitor serial screen 30, and the external capacitor serial screen 30 is used for realizing man-machine interaction and data display;

[0093] The debugging serial port 190 is used for debugging the main control circuit system 10.

[0094] The Bluetooth communication circuit 111 is in communication connection with the main control board 110 and the external Bluetooth device 40, and is used for transmitting working states of various modules and modules in the main control circuit system 10 to the external Bluetooth device 40 during debugging.

[0095] The RS485 communication circuit 112 includes an SP3485 chip, and is in communication connection with the main control board 110, the temperature control board 120 and the reagent input control board 130, and is used for realizing communication between the main control board 110 and the temperature control board 120 and the reagent input control board 130.

[0096] Further, the serial screen communication circuit is mainly connected with a 7-inch serial screen, and man-machine interaction operation and system process control can be realized through the serial screen.

[0097] The Bluetooth communication circuit and the debugging serial port are mainly used for feeding back relevant working states and flag information during design debugging, so as to provide convenience for researchers to check programs.

[0098] The RS485 communication circuit is mainly composed of an SP3485 chip, is mainly connected with the temperature control board and the reagent input control board, and performs mutual communication operation, and is controlled by the main control board.

[0099] In order to make the related technical personnel more easily understand the protection scope and main purpose of the utility model, the operation process of the utility model is described in detail below by combining with a specific embodiment:

[0100] After the system initialization is completed, the FISH equipment operator needs to edit the program (reagent processing steps, reagent temperature, liquid reagent amount, processing time, etc.) according to the requirements, or uses the system default program process, and after clicking start, the system gradually performs operation. The following takes the first step: baking (80 DEG C, lasting 40 min) and the second step: dewaxing (adding 50mL, 68 DEG C, lasting 3 min) as an example to control and describe the process.

[0101] (1) Baking, the temperature control board is informed of the reaction tank setting temperature and the duration time by the 485 communication mode, and after the time reaches, the temperature control is stopped.

[0102] (2) Dewaxing, control reagent input board open dewaxing agent corresponding pipeline valve, drive diaphragm pump to carry out liquid, collect T type sensor to judge whether dewaxing agent flows through liquid inlet pipeline in time, with the promotion of dewaxing agent liquid level in reaction tank, main control board detects photoelectric sensor constantly, judges whether dewaxing agent liquid level reaches the set level, after reagent liquid is finished, inform reagent input board to close pipeline valve. After that, send set temperature and duration to temperature control board, receive real-time control temperature in the period, and display and control to serial screen. After processing is completed, inform reagent input board to start drain valve and pipeline liquid monitoring of drainage again, until drainage is finished.

[0103] (3) According to different reagent processes, processing, operation and real-time state feedback are carried out.

[0104] The above is only the preferred specific embodiment of the present application, but the protection scope of the present application is not limited to this, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application.

Claims

1. A main control circuit system for a FISH device, characterized in that, include: Main control board, temperature control board and reagent input control board that are communicatively connected to the main control board, and diaphragm pump module that is electrically connected to the main control board; The temperature control board is used to adjust the temperature of the reaction tank after receiving the preset temperature and temperature control duration sent by the main control board, and to feed back the real-time temperature of the reaction tank to the main control board. The reagent input control board is used to exchange data with the main control board and to send speed adjustment signals back to the diaphragm pump module through the main control board; The diaphragm pump module is used to receive speed control signals and start or stop pumping reagents into or out of the reaction vessel.

2. The main control circuit system according to claim 1, characterized in that, The diaphragm pump module includes: two diaphragm pumps for pumping reagents into or out of the reaction tank, a diaphragm pump control module electrically connected to the diaphragm pumps and the main control board, and a diaphragm pump sampling module. The diaphragm pump control module is used to receive speed adjustment signals transmitted from the main control board to control one of the diaphragm pumps to pump reagents into or out of the reaction tank. The diaphragm pump sampling module is used to sample the operating current of the two diaphragm pumps.

3. The main control circuit system according to claim 2, characterized in that, The diaphragm pump control module includes two diaphragm pump interfaces, one end of which is electrically connected to the two diaphragm pumps respectively, and the other end is electrically connected to the main control board through a signal buffer.

4. The main control circuit system according to claim 3, characterized in that, Both diaphragm pump interfaces include a first pin, a second pin, and a third pin. The first pin is used to receive the speed regulation signal, the second pin is electrically connected to the diaphragm pump sampling module, and the third pin is electrically connected to the positive terminal of an external 24V power supply. The diaphragm pump sampling module includes: a first sampling circuit and a second sampling circuit; The first sampling circuit includes: a first resistor, a second resistor, a current amplifier, and a first capacitor; The current amplifier includes: a positive input terminal, an inverting input terminal, an output terminal, a positive power supply terminal, a negative power supply terminal, and a reference voltage pin. One end of the first resistor is electrically connected to the positive input terminal of the current amplifier and the second pin of one of the diaphragm pump interfaces, and the other end is electrically connected to the inverting input terminal of the current amplifier and grounded, for sampling the operating current of the diaphragm pump. The output terminal of the current amplifier is grounded through the second resistor, the positive terminal of the power supply is electrically connected to the external power supply, and the negative terminal of the power supply is shorted to the reference voltage pin and then grounded, and electrically connected to the external power supply through the first capacitor. The second sampling circuit includes: an operational amplifier, a third resistor, a fourth resistor, a fifth resistor, and a second capacitor; The positive input terminal of the operational amplifier is electrically connected to the second pin of another diaphragm pump interface, the negative input terminal is electrically connected to one end of the fourth resistor and the fifth resistor respectively, and the output terminal is electrically connected to the main control board through the third resistor. The end of the third resistor that is electrically connected to the main control board is also grounded through the second capacitor. The other end of the fourth resistor is electrically connected to the output of the operational amplifier, and the other end of the fifth resistor is grounded.

5. The main control circuit system according to claim 1, characterized in that, The system also includes a liquid detection module, which is communicatively connected to the reagent input control board via the main control board; The liquid detection module includes: a T-type sensor signal acquisition module for sending a first detection signal when there is liquid in the inlet and outlet pipes of the reaction tank, and a liquid level sensor signal acquisition module for sending a second detection signal when the liquid level in the reaction tank reaches a preset level. The T-type sensor signal acquisition module and the liquid level sensor signal acquisition module are set up independently; The T-type sensor signal acquisition module includes two photoelectric level switches, which are respectively installed on the inlet and outlet pipes of the reaction tank. The liquid level sensor signal acquisition module includes: a photoelectric conical liquid level sensor, which is installed at a preset liquid level in the reaction tank.

6. The main control circuit system according to claim 5, characterized in that, The T-type sensor signal acquisition module is electrically connected to the main control board through a first control circuit; The liquid level sensor signal acquisition module is electrically connected to the main control board through a second control circuit. The reagent input control board is also used to send on or off signals to the first control circuit and / or the second control circuit via the main control board to start or stop the T-type sensor signal acquisition module and / or the liquid level sensor signal acquisition module.

7. The main control circuit system according to claim 1, characterized in that, The system also includes: a magnetic stirring module, comprising a magnetic stirrer for stirring and mixing reagents and a motor interface electrically connected to the magnetic stirrer; One end of the motor interface is electrically connected to the magnetic stirrer, and the other end is electrically connected to the main control board; The main control board is also used to send a start signal to the magnetic stirrer through the motor interface after receiving the real-time temperature, so as to control the magnetic stirrer to start stirring and mixing the reagents.

8. The main control circuit system according to claim 5 or 6, characterized in that, The system also includes a power conversion module, which is electrically connected to an external 24V power supply and the main control board, respectively, for supplying power to the main control board, and supplying power through the main control board to the temperature control board, reagent input control board, diaphragm pump module, liquid detection module and magnetic stirring module electrically connected to the main control board.

9. The main control circuit system according to claim 8, characterized in that, The power conversion module includes a 24V / 12V converter, a 12V / 5V converter, and a 5V / 3.3V converter.

10. The main control circuit system according to claim 1, characterized in that, The system also includes: The serial port screen communication circuit is connected to the main control board and the external capacitor serial port screen for communication, and is used to realize data exchange between the main control board and the external capacitor serial port screen. The external capacitor serial port screen is used to realize human-computer interaction and data display. The debug serial port is used for debugging the main control circuit system. The Bluetooth communication circuit is connected to the main control board and external Bluetooth devices to transmit the working status of each module and module in the main control circuit system to the external Bluetooth devices during debugging. The RS485 communication circuit, including the SP3485 chip, is connected to the main control board, temperature control board, and reagent input control board to enable communication between the main control board, temperature control board, and reagent input control board.

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