Light following system of LED array light source of high-throughput living cell imager
By using an LED array light source light following system, the problems of light phototoxicity to cells and light source mismatch in high-throughput live cell imagers have been solved, achieving high-precision, stable and energy-saving imaging effects and simplifying equipment design.
Patent Information
- Application Number
- CN202520217407.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2035-02-12
AI Technical Summary
Existing high-throughput live-cell imaging instruments suffer from problems such as phototoxicity of light to cells and mismatch between the light source and the objective lens during long-term observation, resulting in unclear images and high equipment complexity.
An LED array light source light following system is adopted. The system controls the lighting and turning off of the LED light source by connecting the objective lens to the LED light source. Combined with RS485 conversion circuit, microcontroller circuit and LED constant current drive dimming circuit, the system achieves synchronous movement of the light source and supplementary lighting effect.
It reduces the impact of mechanical vibration on image quality, improves the consistency and repeatability of imaging, reduces equipment costs, increases the reliability and lifespan of the equipment, achieves high-precision imaging and low thermal effects, adapts to different specimen types, and is energy-saving and environmentally friendly.
Smart Images
Figure CN223711917U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the instrument design and control technical field more specifically relates to a kind of high flux live cell imaging instrument LED array light source light following system. BACKGROUND
[0002] Live cell imaging detection technology is a kind of commonly used technology in biology and biomedical field, for observing and recording the structure, function and interaction of cells at the level of live cells. This technology can obtain images and data in real time under the state of live cells, and provides a powerful tool for the field of cell biology, cell signal transduction, drug development, etc. The current traditional live cell imaging detection technology includes fluorescence microscope, confocal microscope, two-photon microscope and other morphological observation equipment, but these microscopic imaging techniques cannot dynamically observe cells for a long time, and the high flux live cell imaging instrument developed on this basis is increasingly favored by scientific researchers. This kind of instrument can be placed in a cell culture box, which can maintain the biological activity of cell samples for a long time, making it possible to observe the dynamic activity of cells for a long time.
[0003] However, the high flux live cell imaging instrument industry is still in its initial stage, and the high flux live cell imaging instrument generally has an automatic control XYZ axis motion component, which can perform microscopic imaging and long-term observation on high flux specimens. Among them, the motion mechanism of the high flux live cell imaging instrument stage is divided into two types. One is that the objective lens position is fixed, and the live cell specimen on the stage is moved to the top of the objective lens and imaged by moving the stage. The second is that the stage and the live cell specimen on it are fixed, and the objective lens and the imaging module are integrated on a moving platform. The objective lens and the imaging module are moved to the required specimen area below and then imaged by moving the moving platform. Obviously, for live cell specimens that are not suitable for moving or shaking, the specimen is fixed and the objective lens is moved, which is the best solution. However, the design and control of the light source required for microscopic imaging are extremely complex. First, the light has phototoxicity to live cells, and long-term light exposure affects the normal physiological state of live cells, and even causes live cells to die. Second, for high flux (large number of samples) imaging and detection, each specimen needs to be imaged with light source illumination, otherwise the image will be unclear or even unable to be imaged.
[0004] Therefore, how to propose a high flux live cell imaging instrument LED array light source light following system to solve the problem of light source compensation and cell phototoxicity during imaging control, improve the stability of light brightness and the effect of light compensation is a problem that needs to be solved by those skilled in the art. UTILITY MODEL CONTENT
[0005] In view of the above, the utility model provides a kind of high flux live cell imaging instrument LED array light source light following system, solve the light following system control imaging when the light supplement of light source and cell phototoxicity problem, improve the stability of light brightness and light supplement effect, to realize the above-mentioned purpose, the utility model uses following technical solutions:
[0006] A kind of high flux live cell imaging instrument LED array light source light following system, including: objective lens, object table, LED light source and light source light following system, the object table and LED light source are relatively fixed, the objective lens moves relative to object table, the objective lens movement stroke is adapted with the LED light source, the light source light following system is respectively connected with the objective lens and LED light source signal, for controlling LED light source to light or close.
[0007] Optionally, the object table is used to place high flux live cell specimen.
[0008] Optionally, the light source light following system includes: main control panel, RS485 conversion circuit, single-chip microcomputer circuit, indicator light circuit and LED constant current drive dimming circuit;
[0009] The main control panel is connected with the single-chip microcomputer circuit by RS485 conversion circuit, and the single-chip microcomputer circuit is connected with the indicator light circuit, LED light source and LED constant current drive dimming circuit.
[0010] Optionally, it further includes: serial port to parallel port circuit, the serial port to parallel port circuit is connected with the single-chip microcomputer circuit, for expanding the IO port of single-chip microcomputer circuit.
[0011] Optionally, the LED light source is LED array circuit.
[0012] Optionally, it further includes power supply circuit, and the power supply circuit includes DC-DC conversion, for converting 12V into 5V and 3.3V, for providing power for single-chip microcomputer circuit, main control panel or LED light source.
[0013] Optionally, the LED array includes H beads, is arranged in M columns and N clusters, M*N=H, each column is connected in the way of common cathode, and each cluster is connected in the way of common anode.
[0014] Optionally, the indicator light circuit adopts green, blue, yellow, red, white light and the IO of single-chip microcomputer with yin and yang alternate connection;
[0015] When the LD4 outputs a low level, the LD1 outputs a high level, the LD2 outputs a low level, the LD3 outputs a low level, and the green light is on; when the LD4 outputs a high level, the LD1 outputs a low level, the LD2 outputs a high level, the LD3 outputs a high level, and the blue light is on; when the LD4 outputs a low level, the LD1 outputs a low level, the LD2 outputs a high level, the LD3 outputs a low level, and the yellow light is on; when the LD4 outputs a high level, the LD1 outputs a high level, the LD2 outputs a low level, the LD3 outputs a high level, and the red light is on; and when the LD4 outputs a low level, the LD1 outputs a low level, the LD2 outputs a low level, the LD3 outputs a high level, and the white light is on.
[0016] Optionally, the single-chip microcomputer circuit is an HC32F460 single-chip microcomputer.
[0017] Compared with the prior art, the high-flux live cell imaging instrument LED array light source light following system has the following beneficial effects:
[0018] The utility model discloses a kind of high-flux live cell imaging instrument LED array light source light following system, comprising: objective lens, object table, LED light source and light source light following system, the object table and LED light source are relatively fixed, the objective lens moves relative to object table, the objective lens movement stroke is adapted with the LED light source, the light source light following system is respectively with the objective lens and LED light source signal connection, for control LED light source lighting or close.This utility model solves the problem of cell phototoxicity by light following system control imaging when light source is lit, solve the problem that light and objective lens are not matched (light source is not above objective lens after objective lens moves) by LED array, ensure that live cell remains healthy and vigor in whole experimental process.Simultaneously, this kind of design also brings following advantages: 1. reduce mechanical vibration: specimen does not move means that the movement of mechanical parts is reduced, light follows lens, control corresponding position's lamp pearl lighting, significantly reduce the influence of mechanical vibration on imaging quality.2. stability and consistency: fixed specimen position can ensure that the position of specimen relative to detector and light source is consistent each time imaging, this helps to improve the consistency and repeatability of imaging.3. simplify system design: the scheme of moving objective lens without moving specimen, while also not moving light source, simplifies system design, reduces the need of complex mechanical structure and driving device.This not only reduces the manufacturing cost of equipment, but also can improve the reliability and life of equipment.4. high-precision imaging: moving objective lens can more accurately control focal plane, so as to realize high-precision imaging. The movement of objective lens can be accurately regulated in micron level, which is very important for capturing the subtle changes of internal structure of cell.5. flexibility: due to the design and control of LED array, objective lens can be freely moved, users can adjust the size and position of imaging area as needed, this flexibility makes the equipment can adapt to different types and sizes of specimen, increases the universality of equipment.6. low heat effect: compared with traditional light source, LED array light source generates less heat, which is very important for live cell imaging. Excessive heat can cause damage to cell, affect the authenticity of experimental results. The low heat effect of LED array light source helps to maintain the physiological state of cell.7. energy saving and environmental protection: LED array light source has high energy conversion characteristics, compared with traditional light source (such as halogen lamp), energy consumption is lower. This not only reduces operating cost, but also helps environmental protection.8. high flexibility and adaptability: the design of moving objective lens and LED array light source makes the system can easily adapt to different types of well plate and specimen size. Whether it is multi-well plate, single-well plate, or other special-shaped container, objective lens and LED array light source can be adjusted as needed to ensure optimal imaging effect. BRIEF DESCRIPTION OF DRAWINGS
[0019] In order to more clearly illustrate the technical schemes in the embodiments of the present application or the prior art, the accompanying drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced as follows. Obviously, the accompanying drawings in the following description only represent some embodiments of the present application, and other accompanying drawings can be obtained by those skilled in the art without any creative effort on the premise that the accompanying drawings are provided.
[0020] Figure 1 The single-chip microcomputer circuit principle diagram provided by the present application.
[0021] Figure 2 The indicator lamp circuit principle diagram provided by the present application.
[0022] Figure 3 The LED array circuit principle diagram provided by the present application.
[0023] Figure 4 The LED constant-current driving dimming circuit principle diagram provided by the present application.
[0024] Figure 5 The serial port to parallel port circuit principle diagram provided by the present application.
[0025] Figure 6 The RS485 conversion circuit principle diagram provided by the present application.
[0026] Figure 7 The power supply circuit principle diagram provided by the present application. DETAILED DESCRIPTION
[0027] The technical schemes in the embodiments of the present application will be described clearly and completely in combination with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments only represent some embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without any creative effort fall within the protection scope of the present application.
[0028] The embodiment of the present application discloses a high-flux live cell imaging instrument LED array light source light following system, which is characterized by comprising: an objective lens, an objective table, an LED light source and a light source light following system, the objective table and the LED light source are relatively fixed, the objective lens moves relative to the objective table, the movement stroke of the objective lens is adapted to the LED light source, the light source light following system is respectively connected with the objective lens and the LED light source signal, and is used for controlling the LED light source to be turned on or turned off.
[0029] Further, the objective table is used for placing high-flux live cell specimens.
[0030] Further, the light source light following system comprises a main control board, an RS485 conversion circuit, a single-chip microcomputer circuit, an indicator light circuit and an LED constant current drive dimming circuit.
[0031] The main control board is signal connected with the single-chip microcomputer circuit through the RS485 conversion circuit, and the single-chip microcomputer circuit is signal connected with the indicator light circuit, the LED light source and the LED constant current drive dimming circuit respectively.
[0032] Further, it further comprises a serial port to parallel port circuit, which is signal connected with the single-chip microcomputer circuit and used for expanding the IO port of the single-chip microcomputer circuit.
[0033] Further, the LED light source is an LED array circuit.
[0034] Further, it further comprises a power supply circuit, which comprises a DC-DC conversion and is used for converting 12V into 5V and 3.3V to provide electric energy for the single-chip microcomputer circuit, the main control board or the LED light source.
[0035] Further, the LED array comprises H LED beads arranged in M columns and N clusters, M*N=H, each column is connected in a common cathode mode, and each cluster is connected in a common anode mode.
[0036] Further, the indicator light circuit adopts green, blue, yellow, red and white color lights which are connected with the IO of the single-chip microcomputer in a yin-yang alternating mode.
[0037] When the LD4 outputs low level, the LD1 outputs high level, the LD2 outputs low level and the LD3 outputs low level, the green light is lighted up; when the LD4 outputs high level, the LD1 outputs low level, the LD2 outputs high level and the LD3 outputs high level, the blue light is lighted up; when the LD4 outputs low level, the LD1 outputs low level, the LD2 outputs high level and the LD3 outputs low level, the yellow light is lighted up; when the LD4 outputs high level, the LD1 outputs high level, the LD2 outputs low level and the LD3 outputs high level, the red light is lighted up; when the LD4 outputs low level, the LD1 outputs low level, the LD2 outputs low level and the LD3 outputs high level, the white light is lighted up.
[0038] Further, the single-chip microcomputer circuit is an HC32F460 single-chip microcomputer.
[0039] In the specific embodiment, an LED array light source light following system of a high-throughput live cell imager comprises an objective lens, an objective table, an LED light source and a light source light following system, the objective table and the LED light source are relatively fixed, the objective lens moves relative to the objective table, the moving stroke of the objective lens is adapted to the LED light source, the light source light following system is signal connected with the objective lens and the LED light source respectively, and is used for controlling the LED light source corresponding to the real-time position of the objective lens to be lighted up or turned off.
[0040] Further, the object table is placed with high-throughput live cell samples.
[0041] Further, the light source light following system comprises a main control board, an RS485 conversion circuit, a single-chip microcomputer circuit, an indicator light circuit and an LED constant current drive dimming circuit.
[0042] The main control board is connected with the single-chip microcomputer circuit through the RS485 conversion circuit, and the single-chip microcomputer circuit is connected with the indicator light circuit, the LED light source and the LED constant current drive dimming circuit.
[0043] Further, it further comprises a serial port to parallel port circuit connected with the single-chip microcomputer circuit for expanding the IO port of the single-chip microcomputer circuit.
[0044] Further, the LED light source is an LED array circuit.
[0045] Further, it further comprises a power supply circuit comprising a DC-DC conversion for converting 12V into 5V and 3.3V to provide power for the single-chip microcomputer circuit, the main control board or the LED light source.
[0046] Further, the LED array comprises H LED beads arranged in M columns and N clusters, M*N=H, each column is connected in a common cathode mode, and each cluster is connected in a common anode mode, if a certain column and cluster of the LED beads are lighted, the single-chip microcomputer circuit controls the IO and PWM outputs of the LED array to be turned on, and the PWM signal is outputted to control the LED beads from the darkest to the brightest with a duty ratio of 0 to 100%.
[0047] Further, the indicator light circuit adopts green, blue, yellow, red and white lights connected with the IO of the single-chip microcomputer in a positive and negative alternating mode; if the green light needs to be lighted, the LD4 outputs a low level, the LD1 outputs a high level, the LD2 outputs a low level, and the LD3 outputs a low level; if the blue light needs to be lighted, the LD4 outputs a high level, the LD1 outputs a low level, the LD2 outputs a high level, and the LD3 outputs a high level; if the yellow light needs to be lighted, the LD4 outputs a low level, the LD1 outputs a low level, the LD2 outputs a high level, and the LD3 outputs a low level; if the red light needs to be lighted, the LD4 outputs a high level, the LD1 outputs a high level, the LD2 outputs a low level, and the LD3 outputs a high level; if the white light needs to be lighted, the LD4 outputs a low level, the LD1 outputs a low level, the LD2 outputs a low level, and the LD3 outputs a high level.
[0048] Further, the indicator light circuit further comprises a breathing light mode, the PWM signal is outputted by the PWM software with a timer interrupt as the timing, and the duty ratio of the PWM signal is changed from low to high and then from high to low in a cycle.
[0049] In the specific embodiment, a high-throughput live cell imaging instrument LED array light source light following system control method comprises:
[0050] The position relationship of the LED light source is stored in the main control board;
[0051] The corresponding position of the LED light source is obtained according to the real-time position of the objective lens;
[0052] The main control board sends the position of the LED light source to the single-chip microcomputer circuit through the RS485 conversion circuit;
[0053] The single-chip microcomputer circuit drives the LED light source according to the position of the LED light source and the LED constant-current driving dimming circuit, controls the LED light source corresponding to the real-time position of the objective lens to turn on and off, and controls the brightness.
[0054] In the specific embodiment, the main purpose of the live cell imaging instrument is to image the special specimen of live cells for a long time, and for high-throughput live cell specimens, the specimen does not move the objective lens, which is the best solution.
[0055] 1) In order not to interfere with the normal activity of the cells, the light source cannot irradiate the cells for a long time, that is, the light source is controlled to turn on synchronously only when the imaging system needs to image or take pictures, and is not turned on at other times;
[0056] 2) Since the objective lens moves between different cell samples, the best solution for the light source is directly above the objective lens. If it is a single light source, the light source must be synchronized with the objective lens to always be directly above the objective lens. However, this linkage requires displacement and a control system for the light source. In addition, since a large area of live cell samples is in between, the synchronous movement of the light source with the movement of the objective lens causes errors, making it difficult to implement, and the vibration caused by multiple moving parts also interferes with the live cell samples.
[0057] Therefore, the utility model provides a solution: a large-area LED array is formed by using multiple LEDs to replace the movement of the light source. The LED array light source can provide a highly uniform light intensity distribution, which is particularly important for live cell imaging. Cells need to be observed under stable lighting conditions to avoid phototoxicity or photobleaching effects. In addition, through precise software calculation and a control system, the LED light source directly above the objective lens is turned on no matter where the objective lens moves, and other light sources in the LED array are not turned on. In this way, the LED array light source does not need to move, solving the problem of asynchronous movement of the objective lens and the single light source, i.e. solving the problem of cell phototoxicity, and solving the problem of mismatch between the light and the objective lens (the light source is not directly above the objective lens).
[0058] Specifically, as a supplemental lighting component in a microscopic imaging system, it primarily addresses the issues of supplemental lighting and cell phototoxicity during imaging controlled by a light-following system. Cells require supplemental lighting during microscopic imaging; however, prolonged exposure to artificial light can affect cell survival and lifespan. By controlling the intensity and duration of the light exposure, the dosage and intensity of light received by the cells can be controlled, effectively controlling the impact of light on cells during culture. An LED array solves the problem of light and objective lens mismatch (the light source is no longer directly above the objective lens after movement). During microscope movement, the light needs to be accurately controlled to illuminate at an appropriate intensity from above. This is achieved by arranging the LED beads in the array at 4.5mm intervals to cover the entire moving area.
[0059] This invention utilizes the circuit principle of LED arrays, adding functions such as adjustable lighting, constant current, and RS485 control. Compared to current LED arrays, its lighting brightness is more stable and does not change with external factors such as voltage and temperature, providing a more stable microscopic light source illumination effect. It also allows for convenient control of individual LEDs to stably illuminate at any desired brightness.
[0060] In a specific implementation, a control method for an LED array light source tracking system in a high-throughput live-cell imaging system includes the following steps: using an HC32F460 microcontroller as the core of this component, such as... Figure 1 As shown, it receives lighting control commands from the main control board, executes them accurately, and returns information; such as Figure 7 As shown, the power supply circuit regulates the 12V power supply to 5V and 3.3V to power the microcontroller and chips; as Figure 6 As shown, it communicates with the main control board via an RS485 conversion circuit; as... Figure 3 As shown, the array of LEDs provides supplementary lighting; for example... Figure 4 As shown, a constant current circuit is used to maintain a constant current to the LED, ensuring a constant light intensity; a microcontroller circuit controls the LED's illumination and outputs a PWM signal to maintain the constant light intensity; as shown... Figure 5 As shown, a serial-to-parallel converter circuit is used to expand the I / O of a microcontroller; for example... Figure 2 As shown, the indicator light circuit is used to display the indication status. Through the indicator light circuit, the 5 indicator lights adopt a clever circuit design with 4 I / O channels, reducing the number of I / O channels used. This embodiment uses the above circuit design, which can reduce component costs, simplify the circuit, make it easy to maintain and manufacture, and uses RS485 communication with strong anti-interference performance and high communication reliability.
[0061] In the specific embodiment, the anode of the LED is turned on through IO and extended IO controlled by the single-chip microcomputer, and then the constant current chip is outputted through the PWM signal and constant current dimming is realized; the LED array adopts 384 lamp beads, which are arranged in 16 columns and 24 clusters, each column is connected in the common cathode mode, and each cluster is connected in the common anode mode, the lamp in the certain column and cluster needs to be lightened, and the single-chip microcomputer controls the IO and PWM output of the LED array to turn on the corresponding signal.
[0062] In the specific embodiment, the indicator light circuit adopts green, blue, yellow, red and white LED lights which are connected with the IO of the single-chip microcomputer in the alternating mode of positive and negative, the green light needs to be lightened, LD4 outputs low level, LD1 outputs high level, LD2 outputs low level, and LD3 outputs low level; the blue light needs to be lightened, LD4 outputs high level, LD1 outputs low level, LD2 outputs high level, and LD3 outputs high level; the yellow light needs to be lightened, LD4 outputs low level, LD1 outputs low level, LD2 outputs high level, and LD3 outputs low level; the red light needs to be lightened, LD4 outputs high level, LD1 outputs high level, LD2 outputs low level, and LD3 outputs high level; and the white light needs to be lightened, LD4 outputs low level, LD1 outputs low level, LD2 outputs low level, and LD3 outputs high level.
[0063] In the specific embodiment, the indicator light can also be controlled to run in the breathing light mode, the software PWM mode is adopted to realize the breathing light mode, the PWM signal output is realized by the timer interrupt as the timing, and then the PWM signal duty cycle is changed from low to high and from high to low, so that the breathing light effect is realized.
[0064] In the specific embodiment, the light following effect is realized, the main control of the living cell imager sends signals through the serial port to RS485, controls the LED light compensation lamp corresponding to the real-time position of the camera to turn on and off, and controls the brightness, the position relationship of the lamp is stored in the main control of the living cell imager in advance, and the turning-on mode of the indicator light is the same.
[0065] The various embodiments in the specification are described in a progressive manner, each embodiment focuses on the difference from other embodiments, and the same and similar parts of each embodiment can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the related parts can be referred to the method part.
[0066] The above description of disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A high-throughput live cell imager LED array light source light following system, characterized in that, It comprises: an objective, a stage, an LED light source and a light source light following system, the stage and the LED light source are relatively fixed, the objective moves relative to the stage, the objective movement stroke is matched with the LED light source, the light source light following system is respectively connected with the objective and the LED light source, and is used for controlling the LED light source to be turned on or turned off.
2. The LED array light source light following system for a high-throughput live cell imager of claim 1, wherein, The stage is used for placing high-throughput live cell samples.
3. The LED array light source light following system for a high-throughput live cell imager of claim 1, wherein, The light source light following system comprises a main control board, an RS485 conversion circuit, a single-chip microcomputer circuit, an indicator light circuit and an LED constant current driving dimming circuit. The main control board is connected with the single-chip microcomputer circuit through the RS485 conversion circuit, and the single-chip microcomputer circuit is connected with the indicator light circuit, the LED light source and the LED constant current driving dimming circuit.
4. The LED array light source light following system for a high-throughput live cell imager of claim 3, wherein, It further comprises: a serial port to parallel port circuit, which is connected with the single-chip microcomputer circuit and is used for expanding the IO port of the single-chip microcomputer circuit.
5. The LED array light source light following system for a high-throughput live cell imager of claim 1, wherein, The LED light source is an LED array circuit.
6. The LED array light source light following system for a high-throughput live cell imager of claim 1, wherein, It further comprises a power supply circuit, which comprises DC-DC conversion, is used for converting 12V into 5V and 3.3V, and is used for providing power for the single-chip microcomputer circuit, the main control board or the LED light source.
7. The LED array light source light following system for a high-throughput live cell imager of claim 5, wherein, The LED array comprises H LED beads arranged in M columns and N clusters, M*N=H, each column is connected in a common cathode mode, and each cluster is connected in a common anode mode.
8. The LED array light source light following system for a high-throughput live cell imager of claim 3, wherein, The indicator light circuit adopts green, blue, yellow, red and white lights which are connected with the IO of the single-chip microcomputer in a positive and negative alternating mode. When LD4 outputs low level, LD1 outputs high level, LD2 outputs low level and LD3 outputs low level, the green light is turned on; when LD4 outputs high level, LD1 outputs low level, LD2 outputs high level and LD3 outputs high level, the blue light is turned on; when LD4 outputs low level, LD1 outputs low level, LD2 outputs high level and LD3 outputs low level, the yellow light is turned on; when LD4 outputs high level, LD1 outputs high level, LD2 outputs low level and LD3 outputs high level, the red light is turned on; and when LD4 outputs low level, LD1 outputs low level, LD2 outputs low level and LD3 outputs high level, the white light is turned on.
9. The LED array light source light following system of the high-throughput live cell imaging instrument according to claim 3, wherein the single-chip microcomputer circuit is an HC32F460 single-chip microcomputer.