Umbilical cord blood separating and preparing bag

Abstract

The utility model relates to the technical field of umbilical cord blood separation, and particularly discloses an umbilical cord blood separation preparation bag which comprises a plurality of blood bags, a main pipeline, a three-way valve, a needle head and a Luer taper. Wherein each blood bag is used for storing umbilical cord blood in the blood collection bag; one end of the main pipeline is communicated with the plurality of blood bags; an outlet of the three-way valve communicates with the main pipeline; the needle is communicated with one inlet of the three-way valve; the Luer taper is communicated with the other inlet of the three-way valve; the Luer taper is used for being connected with an injector so that the injector can be communicated with the blood bag. By means of the arrangement, when the needle head is communicated with the blood collection bag, transfer of umbilical cord blood from the blood collection bag to the blood bag can be completed; when the Luer taper is communicated with the injector, the reagent in the injector can be smoothly injected into the blood bag, leakage is avoided, and the safety of reagent adding is improved. Wherein the reagent is a hematopoietic stem cell separating medium.

Description

TECHNICAL FIELD

[0001] The utility model relates to umbilical cord blood separation technical field, especially to a kind of umbilical cord blood separation preparation bag. BACKGROUND

[0002] After umbilical cord blood is collected into blood collection bag, it needs to be separated into triple bag for storage and transfer. At present, in the process of umbilical cord blood separation, umbilical cord blood in blood collection bag is generally transferred into triple bag by syringe, the whole transfer process is complicated to operate, and the transfer efficiency is affected by the proficiency of operator, in addition, the transfer process is also prone to contamination of umbilical cord blood;Furthermore, the addition of separation liquid or other reagents by syringe needle puncture method, when puncturing the pipeline of blood collection bag, the risk of reagent leakage due to improper puncture is prone to occur.

[0003] Therefore, it is urgent to study a kind of umbilical cord blood separation preparation bag to solve the above problems. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of umbilical cord blood separation preparation bag to solve the problems, such as low umbilical cord blood separation efficiency, easy to be contaminated and reagent leakage in prior art.

[0005] To achieve the above purpose, the utility model adopts the following technical solutions:

[0006] The umbilical cord blood separation preparation bag comprises:

[0007] A plurality of blood bags, each of the blood bags is used to store umbilical cord blood in blood collection bag;

[0008] Main pipeline, one end of the main pipeline is communicated with a plurality of blood bags;

[0009] Three-way valve, the outlet of the three-way valve is communicated with the main pipeline;

[0010] Needle, the needle is communicated with one inlet of the three-way valve;

[0011] Luer connector, the luer connector is communicated with another inlet of the three-way valve;The luer connector is used to be connected with syringe, so that syringe is communicated with the blood bag.

[0012] As an optional technical scheme of the umbilical cord blood separation preparation bag, the umbilical cord blood separation preparation bag further comprises a first blood stop, the first blood stop is arranged in the first pipeline between the needle and the three-way valve, and is used to control the on-off of the first pipeline.

[0013] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the umbilical cord blood separation and preparation bag further comprises a second blood stopper, which is arranged on the second pipeline between the luer joint and the three-way valve and is used for controlling the opening and closing of the second pipeline.

[0014] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the umbilical cord blood separation and preparation bag further comprises a filter, which is arranged on the main pipeline and is located downstream of the three-way valve, and the filter is used for filtering the umbilical cord blood entering the blood bag.

[0015] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the umbilical cord blood separation and preparation bag further comprises a third blood stopper, which is arranged on the main pipeline and is located between the filter and the three-way valve, and the third blood stopper is used for controlling the opening and closing of the main pipeline.

[0016] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the umbilical cord blood separation and preparation bag comprises a plurality of branch pipelines, the outlets of the branch pipelines are communicated with the blood bag, and the inlets of each branch pipeline are communicated with the outlet of the main pipeline.

[0017] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the blood bag comprises a main body part and a bag end part, the bag end part is tapered and gradually decreases in width away from the main body part.

[0018] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the umbilical cord blood separation and preparation bag further comprises a plurality of branch blood stoppers, and each branch pipeline is provided with a branch blood stopper to control the opening and closing of the corresponding branch pipeline.

[0019] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the blood bag is provided with three blood bags, and the umbilical cord blood separation and preparation bag further comprises a four-way valve, an inlet of the four-way valve is communicated with one end of the main pipeline, and three outlets of the four-way valve are correspondingly communicated with three branch pipelines.

[0020] As an optional technical scheme of the umbilical cord blood separation and preparation bag, the main pipeline is provided with a filter, and the main pipeline comprises a first section and a second section, the first section is located between the filter and the three-way valve, and the second section is located between the filter and the four-way valve.

[0021] The utility model has at least the following beneficial effects:

[0022] The utility model provides a kind of umbilical cord blood separation preparation bag, the umbilical cord blood separation preparation bag includes blood bag, main pipeline, three-way valve, needle and luer joint, wherein, needle and luer joint are communicated with main pipeline by three-way valve, main pipeline is communicated with blood bag, so that needle and blood collection bag are communicated, the transfer of umbilical cord blood can be completed;When luer joint and syringe are communicated, reagent such as separation liquid in syringe can be smoothly injected into blood bag, avoid producing leakage, improve the security of reagent addition. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical scheme in the embodiments of the utility model, the drawings needed to be used in the description of the embodiments of the utility model will be simply introduced below, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the utility model and these drawings without creative labor.

[0024] Figure 1 It is the structure schematic diagram of umbilical cord blood separation preparation bag in the embodiments of the utility model.

[0025] In the drawing:

[0026] 100, blood bag; 110, first bag; 120, second bag; 130, third bag; 101, main body part; 102, bag end part;

[0027] 200, main pipeline; 210, first section; 220, second section; 230, third blood stop;

[0028] 300, three-way valve;

[0029] 400, needle; 410, first blood stop; 420, first pipeline;

[0030] 500, luer joint; 510, second blood stop; 520, second pipeline;

[0031] 600, filter;

[0032] 700, four-way valve; 710, branch pipeline;

[0033] 810, first branch blood stop; 820, second branch blood stop; 830, third branch blood stop. DETAILED DESCRIPTION

[0034] Before any embodiments of the application are explained in detail, it is to be understood that the application is not limited in its application to the details of construction and the arrangement of components set forth in the following description or illustrated in the above-described drawings.

[0035] In this application, the terms "include", "comprise" or "have" or any other variations thereof are intended to cover a non-exclusive inclusion, such that processes, methods, articles, or apparatuses that include a list of elements are not limited to those elements, but can include other elements not expressly listed or inherent to such processes, methods, articles, or apparatuses. Without further limitation, an element defined by the phrase "comprising a" does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0036] In this application, the term "and / or", is a description of an associated object, which means that there can be three kinds of relations. For example, A and / or B, can mean that A exists alone, A and B exist together, and B exists alone. In addition, the character " / " in this application generally indicates that the front and rear associated objects are in a "and / or" relationship.

[0037] In this application, the terms "connection", "combination", "coupling", "mounting" can be direct connection, combination, coupling or mounting, or indirect connection, combination, coupling or mounting. Among them, direct connection refers to the connection of two parts or components without the need for an intermediate part, and indirect connection refers to the connection of two parts or components with at least one intermediate part. In addition, "connection" and "coupling" are not limited to physical or mechanical connection or coupling, and can include electrical connection or coupling.

[0038] In this application, those of ordinary skill in the art will understand that the relative terms used in connection with quantities or conditions (such as "about", "approximately", "substantially" and the like) include the values described and have the meaning indicated by the context. For example, the relative terms at least include the degree of error related to the measurement of a specific value, the tolerance caused by manufacturing, assembly, use, etc. related to a specific value. Such terms should also be considered to disclose the range defined by the absolute values of the two endpoints. The relative term can refer to a certain percentage (such as 1%, 5%, 10% or more) of the indicated value. The numerical value without the relative term should also be disclosed as a specific value with a tolerance. In addition, "substantially" when expressing the relative angular positional relationship (for example, substantially parallel, substantially perpendicular), can refer to a certain degree (such as 1 degree, 5 degrees, 10 degrees or more) added or subtracted from the indicated angle.

[0039] In this application, those of ordinary skill in the art will understand that the functions performed by the components can be performed by one component, multiple components, one part, or multiple parts. Similarly, the functions performed by the parts can also be performed by one part, one component, or multiple parts in combination.

[0040] In the present application, the terms "upper", "lower", "left", "right", "front", "back" and the like are described in the orientation and positional relationship shown in the drawings, and should not be understood as limiting the embodiments of the present application. In addition, it is also understood in the context that when referring to one element connected to another element "on" or "under", it can be directly connected to another element "on" or "under" or indirectly connected to another element "on" or "under" through an intermediate element. It should also be understood that the terms "upper", "lower", "left", "right", "front", "back" and the like not only represent the positive direction, but also can be understood as the side direction. For example, the lower side can include the directly below, the left below, the right below, the front below and the back below, etc.

[0041] As shown in Figure 1 The present embodiment provides a cord blood separation preparation bag, which comprises a plurality of blood bags 100, a main pipeline 200, a three-way valve 300, a needle 400 and a luer connector 500. Each blood bag 100 is used for storing cord blood in a blood collection bag; one end of the main pipeline 200 is in communication with the plurality of blood bags 100; the outlet of the three-way valve 300 is in communication with the main pipeline 200; the needle 400 is in communication with one inlet of the three-way valve 300; the luer connector 500 is in communication with another inlet of the three-way valve 300; and the luer connector 500 is used for connecting with a syringe so as to make the syringe in communication with the blood bag 100.

[0042] With the above arrangement, when the needle 400 is in communication with the blood collection bag, the transfer of cord blood from the blood collection bag to the blood bag 100 can be completed; when the luer connector 500 is in communication with the syringe, the reagent in the syringe can be smoothly injected into the blood bag 100, avoiding external leakage and improving the safety of reagent addition. The reagent is a hematopoietic stem cell separation solution.

[0043] In order to prevent the needle 400 and the luer connector 500 from affecting each other, in some embodiments, the cord blood separation preparation bag further comprises a first blood stop 410, which is arranged in a first pipeline 420 between the needle 400 and the three-way valve 300 and is used for controlling the opening and closing of the first pipeline 420. The cord blood separation preparation bag further comprises a second blood stop 510, which is arranged in a second pipeline 520 between the luer connector 500 and the three-way valve 300 and is used for controlling the opening and closing of the second pipeline 520.

[0044] In order to prevent the clots in the cord blood from entering the blood bag 100, the cord blood separation preparation bag further comprises a filter 600, which is arranged in the main pipeline 200 and located downstream of the three-way valve 300, and is used for filtering the cord blood entering the blood bag 100.

[0045] The umbilical cord blood separation and preparation bag further comprises a third blood stop 230 arranged on the main pipeline 200 and located between the filter 600 and the three-way valve 300, and the third blood stop 230 is used to control the opening and closing of the main pipeline 200 to avoid other liquids entering the blood bag 100.

[0046] In order to realize the communication and the position arrangement of each blood bag 100, the umbilical cord blood separation and preparation bag comprises a plurality of branch pipelines 710, the outlet of each branch pipeline 710 is communicated with the blood bag 100, and the inlet of each branch pipeline 710 is communicated with the outlet of the main pipeline 200.

[0047] Considering that the umbilical cord blood is stratified according to specific gravity after centrifugation, the plasma with lighter specific gravity is located in the upper layer, the red blood cells with heavier specific gravity are located in the lower layer, and the white membrane layer between the upper layer and the lower layer is the white blood cell layer (hematopoietic stem cell layer). The preparation and separation of the umbilical cord blood hematopoietic stem cells is to remove most of the plasma and most of the red blood cells by the cell specific gravity after centrifugation, and to retain part of the plasma, part of the red blood cells and most of the white blood cells. Although the specific gravity of the red blood cells is different from that of the white blood cells, the difference is not large, so the red blood cell layer is mixed with the white blood cells with small specific gravity difference.

[0048] The blood bag 100 comprises a main body part 101 and a bag end part 102, the bag end part 102 is tapered and gradually decreases in width away from the main body part 101. In use, the blood bag 100 is used with the tip downward, and the tapered structure reduces the cross section of the red blood cell layer, increases the height of the red blood cell layer, increases the specific gravity gradient, reduces the mixed white blood cells in the bottom red blood cells, and reduces the mixed white blood cells in the upper red blood cells with small specific gravity difference. The upper red blood cells are retained, the lower red blood cells are removed, the outflow of the hematopoietic stem cells is reduced, and finally the white blood cell recovery rate in the preparation and separation process of the umbilical cord blood hematopoietic stem cells is improved.

[0049] In addition, the tapered structure increases the height of the red blood cell layer, lengthens the distance between the white blood cell layer and the outlet, reduces the influence of the turbulent flow on the white blood cell layer during the separation of the red blood cells, and avoids the loss of the white blood cells.

[0050] During the centrifugation process, the opening of the blood bag 100 needs to be processed to avoid the outflow of the umbilical cord blood, and therefore the umbilical cord blood separation and preparation bag further comprises a plurality of branch blood stops, and each branch pipeline 710 is provided with a branch blood stop to control the opening and closing of the corresponding branch pipeline 710.

[0051] To facilitate the realization of the communication, in some embodiments, the blood bag 100 is provided with three, and the umbilical cord blood separation preparation bag further comprises a four-way valve 700, the inlet of the four-way valve 700 is communicated with one end of the main pipeline 200, and the three outlets of the four-way valve 700 are communicated with the three blood bags 100 one by one. In the embodiment, the blood bag 100 comprises a first bag 110, a second bag 120 and a third bag 130, and the branch blood stop comprises a first branch blood stop 810, a second branch blood stop 820 and a third branch blood stop 830, and the first branch blood stop 810, the second branch blood stop 820 and the third branch blood stop 830 are respectively installed on the three branch pipelines 710 to control the opening and closing of the three branch pipelines 710. Among them, the first branch blood stop 810 corresponds to the first bag 110; the second branch blood stop 820 corresponds to the second bag 120; and the third branch blood stop 830 corresponds to the third bag 130.

[0052] The main pipeline 200 comprises a first section 210 and a second section 220, the first section 210 is located between the filter 600 and the three-way valve 300, and the second section 220 is located between the filter 600 and the four-way valve 700. This kind of setting makes that when the umbilical cord blood and the hematopoietic stem cell separation liquid are filled in the blood bag 100, the heat sealing separation can be realized from the second section 220, that is, the main pipeline 200 is disconnected at the second section 220 by heating.

[0053] The embodiment also discloses a separation preparation method of umbilical cord blood, comprising the following steps:

[0054] The needle 400 is connected to the blood collection bag in the form of puncture.

[0055] The first blood stop 410, the third blood stop 230 and the first branch blood stop 810 are opened, and the umbilical cord blood in the blood collection bag is transferred to the first bag 110 along the main pipeline 200 and one of the branch pipelines 710 by gravity.

[0056] If there is a blood clot in the blood collection bag, the blood clot is filtered and removed by the clot filter 600.

[0057] The syringe containing the hematopoietic stem cell separation liquid is connected with the luer connector 500, the first blood stop 410 is closed, the second blood stop 510 is opened, and the reagent in the syringe is injected into the first bag 110.

[0058] The third blood stop 230 is closed, the second section 220 is heat sealed and separated, the main pipeline 200 above the four-way valve 700 is removed, and each pipeline below the four-way valve 700 is retained to enter the preparation separation.

[0059] The umbilical cord blood is centrifuged, the plasma is separated to prepare the third bag 130, the red blood cells are separated to prepare the second bag 120, and the hematopoietic stem cells are reserved in the first bag 110. Specifically, the first bag 110 is inverted, and the lowermost red blood cells are transferred to the second bag 120 after the first centrifugation process is completed; then the first bag 110 is inverted again with the tip pointing upward, and the second centrifugation is performed; after the second centrifugation is completed, the upper plasma is transferred to the third bag 130 by clamping the first bag 110 with a clamp.

[0060] Obviously, the above embodiments of the present application are merely examples for clearly illustrating the present application, and are not intended to limit the embodiments of the present application. For those skilled in the art, various obvious changes, re-adjustments and substitutions can be made without departing from the protection scope of the present application. It is unnecessary and impossible to enumerate all the embodiments. Any modification, equivalent substitution and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A bag for the separation and preparation of umbilical cord blood, characterized in that, The umbilical cord blood separation and preparation bag comprises: a plurality of blood bags (100), each of which is used for storing umbilical cord blood in a blood collection bag; a main pipeline (200) having one end in communication with the plurality of blood bags (100); a three-way valve (300) having an outlet in communication with the main pipeline (200); a needle (400) in communication with one inlet of the three-way valve (300); a luer connector (500) in communication with another inlet of the three-way valve (300), and used for connecting with a syringe to make the syringe in communication with the blood bag (100).

2. The umbilical cord blood separation and preparation bag of claim 1, wherein, The umbilical cord blood separation and preparation bag further comprises a first blood stopcock (410) arranged in a first pipeline (420) between the needle (400) and the three-way valve (300), and used for controlling opening and closing of the first pipeline (420).

3. The umbilical cord blood separation and preparation bag of claim 2, wherein, The umbilical cord blood separation and preparation bag further comprises a second blood stopcock (510) arranged in a second pipeline (520) between the luer connector (500) and the three-way valve (300), and used for controlling opening and closing of the second pipeline (520).

4. The umbilical cord blood separation and preparation bag of claim 1, wherein, The umbilical cord blood separation and preparation bag further comprises a filter (600) arranged in the main pipeline (200) and located downstream of the three-way valve (300), and used for filtering the umbilical cord blood into the blood bag (100).

5. The umbilical cord blood separation and preparation bag of claim 4, wherein, The umbilical cord blood separation and preparation bag further comprises a third blood stopcock (230) arranged on the main pipeline (200) and located between the filter (600) and the three-way valve (300), and used for controlling opening and closing of the main pipeline (200).

6. The umbilical cord blood separation and preparation bag according to any one of claims 1 to 5, characterized in that, The umbilical cord blood separation and preparation bag comprises a plurality of branch pipelines (710) having outlets in communication with the blood bags (100), and each of the branch pipelines (710) having an inlet in communication with an outlet of the main pipeline (200).

7. The umbilical cord blood separation and preparation bag of claim 6, wherein, The blood bag (100) comprises a main body (101) and a bag end (102), the bag end (102) is tapered and gradually decreases in width away from the main body (101).

8. The umbilical cord blood separation and preparation bag of claim 6, wherein, The umbilical cord blood separation and preparation bag further comprises a plurality of branch blood stopcocks, each of the branch pipelines (710) is provided with a branch blood stopcock to control opening and closing of the corresponding branch pipeline (710).

9. The umbilical cord blood separation and manufacturing bag of claim 6, wherein, The blood bag (100) is provided with three, and the umbilical cord blood separation and preparation bag further comprises a four-way valve (700) having an inlet in communication with one end of the main pipeline (200), and three outlets of the four-way valve (700) corresponding to and in communication with the three branch pipelines (710) respectively.

10. The umbilical cord blood separation and preparation bag of claim 9, wherein, The main pipe (200) is provided with a filter (600), the main pipe (200) comprises a first section (210) and a second section (220), the first section (210) is located between the filter (600) and the three-way valve (300), and the second section (220) is located between the filter (600) and the four-way valve (700).

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