Blood sampling perfusion device for experimental animals

Abstract

The utility model discloses a blood sampling perfusion device for experimental animals. The blood sampling perfusion device comprises a perfusion needle tube, wherein the side wall of the perfusion needle tube is communicated with a formaldehyde input tube and a normal saline input tube; the blood collection needle tube is slidably assembled in the perfusion needle tube, an elastic ball cage is arranged at the first end of the blood collection needle tube, and a sampling tube connector is arranged at the second end; and the elastic ball cage hides or exposes the perfusion needle tube according to the use state. The blood sampling and perfusion device not only improves the efficiency and quality of experimental animal heart blood sampling and perfusion, but also reduces the pain of experimental animals and the complexity of experimental operation, and has a remarkable technical effect of improving the accuracy and efficiency of experimental research.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of experimental device relates to a blood sampling perfusion device for experimental animals, aims at solving the problems of insufficient blood sampling, serious hemolysis and the like in the process of animal model making and sampling in medical experiments. BACKGROUND

[0002] In biological and medical scientific research, the making and sampling of animal models are important experimental links. Blood collection and organ perfusion of animal models are two key steps, which are crucial for the success of subsequent experiments. However, the traditional blood sampling and perfusion methods have many shortcomings, which seriously affect the accuracy and efficiency of experiments.

[0003] At present, the common pump-type perfusion device on the market cannot consider the blood sampling operation, and the perfusion time is long, and the flow rate and flow are not easy to control. In the process of experimental sampling, hemostatic forceps are usually used to fix the needle, and after blood sampling is completed, warm saline is usually injected into the heart again, and then 4℃ polyformaldehyde is injected for perfusion, so as to facilitate the sampling of organs and better maintain the morphological structure of animal organs under the living state.

[0004] In the process of implementing the utility model, the inventors found that there is at least one of the following technical problems in the prior art:

[0005] (1) In medical experiments, the blood collection amount of animal models (such as experimental rats) is often insufficient, and if it is not good, it may only have 1ml, which leads to repeated puncture, which not only increases the pain of animals, but also may cause blood coagulation, affecting the experimental results.

[0006] (2) Due to the limitation of blood sampling technology and the deficiency of blood sampling equipment, hemolysis often occurs, which will seriously affect the quality of blood samples and further affect the accuracy of experimental results.

[0007] (3) In the perfusion process, due to the complexity of the heart structure, such as the existence of valves and papillary muscles, the needle is easy to be blocked, and then the direction needs to be frequently changed during sampling, which increases the damage to the heart tissue and affects the research of heart organs. UTILITY MODEL CONTENTS

[0008] In view of the limitations of the prior art, it is urgent to develop a new type of blood sampling and perfusion device for experimental animals. Such a device should be able to complete blood sampling and perfusion at one time, reduce damage to the animal heart, improve blood sampling efficiency and quality. At the same time, the device should also simplify the experimental operation process, improve the accuracy and efficiency of the experiment, in order to meet the needs of modern biological and medical research.

[0009] The inventor, through long-term exploration and trial, and multiple experiments and efforts, constantly reforms and innovates, and provides the technical scheme in the utility model for solving the above technical problems, and provides a blood sampling and perfusion device for experimental animals, which comprises:

[0010] The perfusion needle tube is provided with a formaldehyde input pipe and a physiological saline input pipe in the side wall.

[0011] The blood sampling needle tube is slidably assembled in the perfusion needle tube, the first end of the blood sampling needle tube is provided with an elastic ball cage, and the second end is provided with a sampling pipe connecting head; the elastic ball cage hides or exposes the perfusion needle tube according to the use state.

[0012] On the basis of the above technical scheme, the utility model can also be improved as follows:

[0013] Further, the elastic ball cage is composed of a telescopic lantern framework structure of multiple elastic sheets.

[0014] Preferably, the elastic sheet is 4-8 sheets.

[0015] Preferably, the elastic sheet is 6 sheets.

[0016] On the basis of the above technical scheme, the utility model can also be improved as follows:

[0017] Further, the diameter of the elastic ball cage after exposing the perfusion needle tube is 1-1.8 times the outer diameter of the perfusion needle tube.

[0018] On the basis of the above technical scheme, the utility model can also be improved as follows:

[0019] Further, a perfusion flow channel is arranged between the perfusion needle tube and the blood sampling needle tube.

[0020] Preferably, a groove is arranged on the contact surface of the elastic sheet and the inner wall of the perfusion needle tube.

[0021] On the basis of the above technical scheme, the utility model can also be improved as follows:

[0022] Further, heparin is arranged in the perfusion needle tube or the blood sampling needle tube.

[0023] On the basis of the above technical scheme, the utility model can also be improved as follows:

[0024] Further, the formaldehyde input pipe is connected with a 4 DEG C polyformaldehyde supply container through a first pipe, and a first flow valve is arranged on the first pipe.

[0025] On the basis of the above technical scheme, the utility model can also be improved as follows:

[0026] Further, the physiological saline input pipe is connected with a 30-37 DEG C physiological saline supply container through a second pipeline, and a second flow valve is installed on the first pipeline.

[0027] Compared with the prior art, the blood sampling and perfusion device improves the efficiency and quality of heart blood sampling and perfusion of experimental animals, reduces the pain of experimental animals and the complexity of experimental operation, and has a significant technical effect on improving the accuracy and efficiency of experimental research:

[0028] a) Since the device can complete blood sampling and perfusion in a disposable operation, repeated puncture of the heart of the experimental animal is avoided, thereby significantly reducing damage to the heart.

[0029] b) The elastic ball cage can be expanded and fixed after puncturing into the left ventricle, preventing the needle from falling off, and preventing the heart cavity valve and structure from blocking the needle, thereby increasing the blood sampling amount and reducing hemolysis.

[0030] c) The perfusion flow passage between the perfusion needle tube and the blood sampling needle tube is designed to allow direct perfusion through the same needle tube after blood sampling, without the need to replace equipment, thereby simplifying the experimental operation process.

[0031] d) The adjustability of the number of elastic sheets and the diameter of the elastic ball cage enables the device to adapt to experimental animals of different sizes, and has good universality and adaptability.

[0032] e) The design of the device reduces the uncertainty of human operation, reduces errors in the experimental process, and improves the accuracy of experimental results.

[0033] f) By accurately controlling the temperature and flow rate of the perfusion liquid, the morphological structure of the animal organs under the living state can be better maintained, which is of great significance for subsequent pathological analysis and research.

[0034] g) The built-in heparin design reduces blood coagulation in the needle tube, and the direct use of pre-cooled paraformaldehyde and pre-heated physiological saline saves the pre-heating and pre-cooling time in the experiment, improving the experimental efficiency.

[0035] h) By installing a first flow valve and a second flow valve on the formaldehyde input pipe and the physiological saline input pipe respectively, the flow rate and flow of the perfusion liquid can be accurately controlled, improving the controllability and repeatability of the experiment. BRIEF DESCRIPTION OF DRAWINGS

[0036] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings used in the embodiments will be briefly introduced as follows. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be considered as a limitation to the scope. Other related drawings can also be obtained by those skilled in the art without creative effort, under the premise of not paying creative effort.

[0037] Figure 1 is a front view structural schematic diagram of a preferred embodiment of the blood sampling and perfusion device for laboratory animals.

[0038] Figure 2 is a right view structural schematic diagram of Figure 1 .

[0039] Figure 3 is an A-A cross-sectional view schematic diagram of Figure 2 .

[0040] Figure 4 is a three-dimensional structural schematic diagram of Figure 1 .

[0041] Figure 5 is a three-dimensional structural schematic diagram of the elastic ball cage in a storage state of the blood sampling and perfusion device for laboratory animals.

[0042] The marks in the drawings are as follows:

[0043] 100 perfusion needle tube,

[0044] 110 physiological saline input tube,

[0045] 120 formaldehyde input tube,

[0046] 200 blood sampling needle tube,

[0047] 210 elastic ball cage,

[0048] 211 elastic sheet,

[0049] 220 sampling tube connector,

[0050] 300 perfusion flow channel. DETAILED DESCRIPTION

[0051] The embodiments will be described below in conjunction with the drawings and a specific embodiment.

[0052] In order to make the purposes, technical schemes and advantages of the embodiments of the present application clearer, the technical schemes of the embodiments of the present application will be described clearly and completely below in conjunction with the drawings of the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application.

[0053] It should be noted that similar reference numerals and letters refer to similar items in the drawings, and thus, once an item is defined in one drawing, it can not be further defined and explained in subsequent drawings.

[0054] Referring to Figures 1 to 5 The experimental animal blood sampling and perfusion device described in the present embodiment comprises a perfusion needle tube 100 and a blood sampling needle tube 200, and is suitable for heart blood sampling and organ perfusion of experimental animals such as rats.

[0055] The side wall of the perfusion needle tube 100 is communicated with a formaldehyde input tube 120 and a physiological saline input tube 110. The design of the perfusion needle tube 100 needs to ensure that the side wall thereof can stably and sealingly communicate the formaldehyde input tube 120 and the physiological saline input tube 110. The material of the perfusion needle tube 100 should be selected from materials with good biocompatibility and chemical corrosion resistance, such as medical-grade stainless steel or plastic.

[0056] Two supply containers (conventional containers, not shown in the drawings) are prepared, one for storing 4℃ polyformaldehyde and the other for storing 30-37℃ physiological saline. These containers should have a heat preservation function to maintain the temperature of the liquid stable. The polyformaldehyde supply container should have good sealing performance to prevent formaldehyde from volatilizing. The physiological saline supply container should be kept clean to ensure that the physiological saline is not contaminated.

[0057] Flow valves (conventional flow valves, not shown in the figure) are installed on the formaldehyde input pipe 120 and the physiological saline input pipe 110, respectively. These flow valves can be manually adjusted or electronically controlled to achieve precise flow rate and flow control. The selection of the flow valve should consider the convenience and accuracy of operation, as well as the compatibility with the supply container and the perfusion needle pipe 100. Any flow valve used in the prior art can be used in the device of the present application. Before the experiment begins, the flow rate and flow of the flow valve are set according to the body weight and heart size of the experimental animal. The adjustment of the flow valve should ensure that the perfusion solution can flow uniformly and continuously into the heart, avoiding too fast or too slow flow rate causing heart injury or insufficient perfusion. Before the formal experiment, the temperature and pressure test of the perfusion solution should be carried out to ensure that the temperature and pressure of the perfusion solution meet the experimental requirements when flowing into the heart. This can be monitored in real time by connecting a pressure sensor and a thermometer to the supply container and the perfusion needle pipe 100.

[0058] The blood collection needle pipe 200 is slidingly assembled in the perfusion needle pipe 100, the first end of the blood collection needle pipe 200 is provided with an elastic ball cage 210, and the second end is provided with a sampling pipe connector 220; the elastic ball cage 210 hides or exposes the perfusion needle pipe 100 according to the use state. The blood collection needle pipe 200 is designed to be smoothly slidingly assembled in the perfusion needle pipe 100. This assembly allows the blood collection needle pipe 200 to move freely inside the perfusion needle pipe 100, so that the position of the blood collection needle pipe 200 can be adjusted as needed. During assembly, ensure that the connection part of the two needle pipes is sealed well to prevent liquid leakage during operation. The first end of the blood collection needle pipe 200 is provided with an elastic ball cage 210, which is composed of six elastic springs 211, forming an extensible lantern framework structure. The first end of the six elastic springs 211 is connected with the blood collection needle pipe 200, and the second end of the six elastic springs 211 is connected and structured as a smooth curved surface. The design of the elastic ball cage 210 allows it to hide in the inner wall of the perfusion needle pipe 100 when not in use, facilitating the puncture operation.

[0059] The second end of the blood collection needle pipe 200 is provided with a sampling pipe connector 220, which is used to connect an external blood collection container or blood collection system. The sampling pipe connector 220 should be designed to be easy to connect and disconnect, while ensuring the sealing and sterility of the connection.

[0060] During use, the state of the elastic ball cage 210 is adjusted as needed. When blood collection is needed, the blood collection needle tube 200 is operated so that the elastic ball cage 210 extends out of the perfusion needle tube 100 and is expanded to form a larger opening, so that blood flows smoothly into the blood collection needle tube 200, and also facilitates the flow of formaldehyde and physiological saline into the heart chamber. After perfusion is completed, the elastic ball cage 210 can be retracted and hidden in the perfusion needle tube 100 to reduce interference with the internal structure of the heart. Before actual use, a test operation is performed to ensure that the elastic ball cage 210 can be smoothly extended and retracted, and can stably maintain its shape in the extended state to prevent the heart tissue from blocking the needle.

[0061] In the blood collection operation, first, the perfusion needle tube 100 is punctured into the heart of the experimental animal, such as a rat, and then the blood collection needle tube 200 is pushed to extend the elastic ball cage 210 and expand it, and the diameter of the elastic ball cage 210 exposed after the perfusion needle tube 100 is 1-1.8 times the outer diameter of the perfusion needle tube 100, and blood collection begins. After blood collection is completed, perfusion is performed, and after perfusion is completed, the elastic ball cage 210 is retracted into the perfusion needle tube 100.

[0062] After the blood collection and perfusion operations are completed, the blood collection needle tube 200 is disassembled and cleaned and disinfected for next use. The integrity and elasticity of the elastic ball cage 210 are checked to ensure that its function is not impaired.

[0063] In further embodiments, a perfusion flow channel 300 is provided between the perfusion needle tube 100 and the blood collection needle tube 200. Preferably, the contact surface of the elastic sheet 211 with the inner wall of the perfusion needle tube 100 is provided with a groove.

[0064] In further embodiments, in order to prevent blood clotting in the perfusion needle tube 100 or the blood collection needle tube 200, an anticoagulant heparin can be applied to the inner walls of the two needle tubes. The heparin suitable for medical use is usually heparin sodium or heparin calcium. This anticoagulant can prevent blood from clotting in the needle tube, keep the blood flowing smoothly, and reduce hemolysis during blood collection. The heparin is dissolved in a suitable solvent, usually physiological saline or sterile water, to form a heparin solution. The concentration of the heparin solution is adjusted as needed, and the general concentration range can be between 1 unit / ml and 10 units / ml.

[0065] In the description of the utility model, it is understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like is the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model.

[0066] In addition, the terms "first" and "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" can explicitly or implicitly include one or more of the features. In the description of the utility model, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0067] In the utility model, unless otherwise specifically defined and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood in a broad sense, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication or interaction relationship between two elements. For ordinary skilled persons in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0068] In the utility model, unless otherwise specifically defined and limited, the "upper" or "lower" of the first feature to the second feature can include the direct contact of the first and second features, or it can include the contact of the first and second features through another feature between them. Moreover, the "upper", "upper" and "upper" of the first feature to the second feature include the vertical direction of the first feature above and obliquely above the second feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The "below", "below" and "below" of the first feature to the second feature include the vertical direction of the first feature below and obliquely below the second feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.

[0069] The above is only the preferred embodiment of the utility model, and it should be pointed out that the above preferred embodiment should not be regarded as limiting the utility model, and the protection scope of the utility model should be limited by the scope defined in the claims. For ordinary skilled persons in the art, without departing from the spirit and scope of the utility model, a number of improvements and refinements can be made, which should also be regarded as the protection scope of the utility model.

Claims

1. A blood collection perfusion device for laboratory animals, characterized by The application relates to a perfusion needle tube and a blood sampling needle tube. The perfusion needle tube is provided with a formaldehyde input pipe and a physiological saline input pipe. The blood sampling needle tube is slidably arranged in the perfusion needle tube, the first end of the blood sampling needle tube is provided with an elastic ball cage, and the second end is provided with a sampling pipe connector; the elastic ball cage hides or exposes the perfusion needle tube according to the use state.

2. The blood perfusion apparatus for experimental animals according to claim 1, wherein The elastic ball cage is composed of multiple elastic sheets and has a telescopic lantern framework structure.

3. The blood perfusion apparatus for experimental animals according to claim 2, wherein The elastic sheet is 4-8 sheets.

4. The blood perfusion apparatus for experimental animals according to claim 3, wherein The elastic sheet is 6 sheets.

5. The blood perfusion apparatus for experimental animals according to claim 1, wherein The diameter of the elastic ball cage after exposing the perfusion needle tube is 1-1.8 times the outer diameter of the perfusion needle tube.

6. The blood perfusion apparatus for experimental animals according to claim 2, wherein A perfusion flow channel is arranged between the perfusion needle tube and the blood sampling needle tube.

7. The blood perfusion apparatus for experimental animals according to claim 6, wherein Grooves are arranged on the contact surface of the elastic sheet and the inner wall of the perfusion needle tube.

8. The blood perfusion apparatus for experimental animals according to claim 1, wherein Heparin is arranged in the perfusion needle tube or the blood sampling needle tube.

9. The blood perfusion apparatus for experimental animals according to claim 1, wherein The formaldehyde input pipe is connected with a 4 DEG C polyformaldehyde supply container through a first pipe, and a first flow valve is arranged on the first pipe.

10. The blood perfusion apparatus for experimental animals according to claim 1, wherein The physiological saline input pipe is connected with a 30-37 DEG C physiological saline supply container through a second pipe, and a second flow valve is arranged on the first pipe.

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