Liquid nitrogen equipment for biological sample storage

Abstract

The utility model relates to the technical field of liquid nitrogen cryopreservation, and discloses liquid nitrogen equipment for biological sample storage, which comprises a first liquid nitrogen tank, a second liquid nitrogen tank is arranged on the right side of the first liquid nitrogen tank, and a nitrogen generator is arranged on the right side of the second liquid nitrogen tank. Pipelines are connected among the first liquid nitrogen tank, the second liquid nitrogen tank and the nitrogen generator; the fan-shaped test tube rack is circumferentially inserted into the first liquid nitrogen tank, bearing plates are connected to the positions, located on the lower portion of the fan-shaped test tube rack, of an inner cavity of the first liquid nitrogen tank, high-elasticity shielding cloth is connected to the two sides of the exterior of the fan-shaped test tube rack, and the outer ends of the high-elasticity shielding cloth are connected with the inner wall of the first liquid nitrogen tank. According to the liquid nitrogen equipment for biological sample storage, through the additionally arranged multiple sets of fan-shaped test tube racks, storage and taking operation of multiple samples can be conducted at the same time, the taking time is shortened, and meanwhile through high-elasticity shielding cloth connected to the two sides of the fan-shaped test tube racks, leakage of liquid nitrogen is prevented.

Description

TECHNICAL FIELD

[0001] The utility model relates to liquid nitrogen cryopreservation technical field, specifically for a kind of liquid nitrogen equipment for biological sample storage. BACKGROUND

[0002] With the development of biotechnology, the storage of biological samples has become an indispensable part. Biological samples include cells, tissues, blood products, vaccines, etc., and have wide applications in the fields of life science research, new drug development, clinical sample library construction, stem cell therapy, precision medicine, etc. These biological samples need to be stored in a low-temperature environment to slow down or stop their metabolic activities, thereby extending their storage time.

[0003] The common liquid nitrogen equipment for biological sample storage includes a liquid nitrogen tank and a test tube rack. When in use, the frozen cells are first placed in a test tube, and the test tube is placed on the storage rack. The entire storage rack is then placed in the liquid nitrogen storage tank to achieve long-term stable storage of the sample. However, this method requires the addition of a sealed door to the liquid nitrogen tank for taking out and placing the test tube rack. When accessing the biological sample test tube, the storage tank needs to be opened, and then the storage rack is taken out. During the process of taking out the storage rack, liquid nitrogen will overflow from the opening of the storage tank, affecting the temperature and liquid nitrogen inside the liquid nitrogen tank. Moreover, the liquid nitrogen that flows out after opening the door can directly contact the operator, increasing the risk of liquid nitrogen frostbite and failing to meet the working requirements of liquid nitrogen cryopreservation. Therefore, a liquid nitrogen equipment for biological sample storage is proposed. SUMMARY

[0004] (I) Technical problem solved

[0005] To solve the technical problems of liquid nitrogen overflowing from the opening of the storage tank when the storage tank is opened, affecting the temperature and liquid nitrogen inside the liquid nitrogen tank, and increasing the risk of liquid nitrogen frostbite, the utility model provides a liquid nitrogen equipment for biological sample storage.

[0006] (II) Technical solution

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a liquid nitrogen equipment for biological sample storage, comprising:

[0008] A first liquid nitrogen tank is provided with a second liquid nitrogen tank on the right side. A nitrogen generator is provided on the right side of the second liquid nitrogen tank. The first liquid nitrogen tank, the second liquid nitrogen tank, and the nitrogen generator are all connected by pipelines.

[0009] A siphon pipe is installed on the top of the first liquid nitrogen tank. The top end of the siphon pipe and the top of the second liquid nitrogen tank are both provided with a connecting pipe. The bottom of the connecting pipe is provided with a refrigerator. The refrigerators are all connected by a water chiller through a pipeline.

[0010] The fan-shaped test tube rack is circumferentially inserted into the first liquid nitrogen tank, the inner cavity of the first liquid nitrogen tank is located at the lower part of the fan-shaped test tube rack, the supporting plates are connected to the positions of the lower part of the fan-shaped test tube rack, high-elasticity shielding cloths are connected to the outer sides of the fan-shaped test tube rack, and the outer ends of the high-elasticity shielding cloths are connected to the inner walls of the first liquid nitrogen tank.

[0011] Preferably, the number of the fan-shaped test tube racks is 3-5 groups, arc-shaped plates are installed at the outer ends of the fan-shaped test tube racks, handles are installed at the outer sides of the middle parts of the arc-shaped plates, and test tube insertion holes are formed in the inner parts of the fan-shaped test tube racks, so that the sample test tubes are conveniently inserted and installed.

[0012] Preferably, assembly sleeves are installed at the positions corresponding to the arc-shaped plates on the outer upper sides of the first liquid nitrogen tank, and insertion sleeves are installed at the positions corresponding to the arc-shaped plates on the outer upper sides of the arc-shaped plates.

[0013] Preferably, insertion rods are inserted into the inner parts of the assembly sleeves, the top ends of the insertion rods penetrate through the top parts of the assembly sleeves, and the outer lower sides of the insertion rods are connected with the lever rods, so that the movement of the insertion rods is driven by the lever rods, the insertion rods are inserted into the inner parts of the insertion sleeves, the arc-shaped plates and the fan-shaped test tube racks are limited, and the sealing performance of the first liquid nitrogen tank is improved.

[0014] Preferably, T-shaped through grooves are formed in the outer lower sides of the assembly sleeves, discs are sleeved on the outer parts of the insertion rods at the lower sides of the inner cavities of the assembly sleeves, and springs are sleeved on the outer parts of the insertion rods between the discs and the top parts of the inner cavities of the assembly sleeves, so that the insertion rods are conveniently moved up and down, and the lever rods are conveniently limited.

[0015] Preferably, sliding grooves are formed in the inner middle parts of the supporting plates, sliding blocks are installed at the middle parts of the bottoms of the fan-shaped test tube racks, the sliding blocks are inserted into the inner parts of the sliding grooves, and the shapes of the sliding grooves and the sliding blocks are trapezoidal, so that the stability of the fan-shaped test tube racks is improved.

[0016] (Three) beneficial effects

[0017] Compared with the prior art, the liquid nitrogen equipment for biological sample storage has the following beneficial effects:

[0018] The liquid nitrogen equipment for biological sample storage, through the added multiple groups of fan-shaped test tube racks, can simultaneously perform multiple sample access operations, reduce the taking time, and avoid cross contamination between different samples in the access process due to the mutual independence of each channel, guarantee the purity and quality of the samples, and through the high-elasticity shielding cloth connected on both sides of the fan-shaped test tube rack, the high-elasticity shielding cloth can be stretched when the fan-shaped test tube rack is pulled out, so that the outlet of the first liquid nitrogen tank can be shielded by the high-elasticity shielding cloth when the fan-shaped test tube rack is pulled out, preventing the leakage of liquid nitrogen, reducing the volatilization speed of liquid nitrogen, saving the cost of liquid nitrogen, reducing the workload of frequent addition of liquid nitrogen, and the high-elasticity shielding cloth can avoid the direct contact of the operator with liquid nitrogen when taking the biological test tube sample, reducing the risk of liquid nitrogen frostbite, improving the safety and reliability of the equipment. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the utility model;

[0020] Figure 2 It is a structural schematic diagram of the water chiller unit of the utility model;

[0021] Figure 3 It is a structural schematic diagram of the first liquid nitrogen tank and the fan-shaped test tube rack of the utility model;

[0022] Figure 4 It is a structural schematic diagram of the bearing plate of the utility model;

[0023] Figure 5 It is a structural schematic diagram of the sliding block of the utility model;

[0024] Figure 6 It is a structural schematic diagram of the assembly sleeve and the insertion rod of the utility model;

[0025] Figure 7 It is a sectional view of the assembly sleeve of the utility model.

[0026] In the figure: 1, first liquid nitrogen tank; 2, second liquid nitrogen tank; 3, nitrogen generator; 4, pipeline; 5, siphon; 6, connecting pipe; 7, refrigeration machine; 8, water chiller unit; 9, fan-shaped test tube rack; 10, arc plate; 11, bearing plate; 12, high-elasticity shielding cloth; 13, assembly sleeve; 131, insertion sleeve; 14, sliding groove; 15, sliding block; 16, insertion rod; 17, lever; 18, through slot; 19, disc; 20, spring. DETAILED DESCRIPTION

[0027] Clearly and completely describe the technical scheme in the embodiments of the utility model with reference to the drawings in the embodiments of the utility model, obviously, the described embodiments are only a part of the embodiments of the utility model, and not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skill in the art without creative labor belong to the scope of the utility model protection.

[0028] The utility model provides a kind of technical scheme, a liquid nitrogen equipment for biological sample storage, including first liquid nitrogen tank 1, second liquid nitrogen tank 2, nitrogen generator 3, pipeline 4, siphon 5, connecting pipe 6, refrigerator 7, water chiller 8, fan-shaped test tube rack 9, arc plate 10, support plate 11, high elasticity shielding cloth 12, assembly set 13, insert sleeve 131, sliding slot 14, sliding block 15, plug rod 16, lever 17, through slot 18, disc 19 and spring 20:

[0029] Please refer to Figure 1 First liquid nitrogen tank 1, the right side of first liquid nitrogen tank 1 is equipped with second liquid nitrogen tank 2, the right side of second liquid nitrogen tank 2 is equipped with nitrogen generator 3, and the top of nitrogen generator 3 is equipped with air compressor, pipeline 4 is connected between first liquid nitrogen tank 1, second liquid nitrogen tank 2 and nitrogen generator 3, and the upper side between first liquid nitrogen tank 1 and second liquid nitrogen tank 2 is connected with circulating pipe, and the middle part of pipeline 4 is equipped with valve;

[0030] Siphon 5 is installed at the top of first liquid nitrogen tank 1, and the top of siphon 5 and second liquid nitrogen tank 2 is equipped with connecting pipe 6, and the bottom of connecting pipe 6 is equipped with refrigerator 7, please refer to Figure 2 Refrigerator 7 is installed with water chiller 8 through pipeline 4, liquid nitrogen in second liquid nitrogen tank 2 is transported to first liquid nitrogen tank 1 through pipeline 4, and first liquid nitrogen tank 1 is timely supplemented with liquid nitrogen, the temperature in first liquid nitrogen tank 1 is guaranteed, so as to ensure the safety of biological sample, and the nitrogen gas evaporated in first liquid nitrogen tank 1 can be discharged into second liquid nitrogen tank 2 through circulating pipe, and the evaporated nitrogen gas can be re-pressurized and cooled to liquid nitrogen, realizing the recycling of liquid nitrogen;

[0031] Please refer to Figure 3 Fan-shaped test tube rack 9 is peripherally inserted in the inside of first liquid nitrogen tank 1, please refer to Figure 4 The inner cavity of first liquid nitrogen tank 1 is connected with support plate 11 at the position below fan-shaped test tube rack 9, sliding slot 14 is formed in the inside middle position of support plate 11, please refer to Figure 5 The middle position of the bottom of fan-shaped test tube rack 9 is installed with sliding block 15, and sliding block 15 is inserted in the inside of sliding slot 14, and the shape of sliding slot 14 and sliding block 15 is trapezoidal, please refer to Figure 4The fan-shaped test tube rack 9 has high-elasticity shielding cloth 12 connected to both sides of its exterior. The outer ends of the high-elasticity shielding cloth 12 are connected to the inner wall of the first liquid nitrogen tank 1. There are 3-5 sets of fan-shaped test tube racks 9. Each fan-shaped test tube rack 9 has an arc-shaped plate 10 installed at its outer end. Each arc-shaped plate 10 has a handle installed in the middle of its outer side. Each fan-shaped test tube rack 9 has a test tube insertion hole inside. By adding multiple sets of fan-shaped test tube racks 9, multiple samples can be stored and retrieved simultaneously, reducing retrieval time. Furthermore, because each channel is independent, cross-contamination between different samples can be avoided during storage and retrieval, ensuring the purity and quality of the samples. Meanwhile, the highly elastic shielding cloth 12 connected to both sides of the fan-shaped test tube rack 9 allows it to extend and retract when pulled out. This shielding cloth 12 effectively covers the outlet of the first liquid nitrogen tank 1 when the fan-shaped test tube rack 9 is opened, preventing leakage of liquid nitrogen. This not only reduces the evaporation rate of liquid nitrogen and saves on costs, but also reduces the workload of frequent liquid nitrogen replenishment. Furthermore, the added highly elastic shielding cloth 12 prevents operators from directly contacting liquid nitrogen when handling biological test tube samples, reducing the risk of liquid nitrogen frostbite and improving the safety and reliability of the equipment. Please refer to [link / reference]. Figure 3 and Figure 4 The upper outer side of the first liquid nitrogen tank 1 is equipped with a mounting bracket 13 at a position corresponding to the arc-shaped plate 10, and the upper outer side of the arc-shaped plate 10 is equipped with a insert 131 at a position corresponding to the mounting bracket 13. Please refer to [link / reference]. Figure 6 and Figure 7 Each of the components 13 has a rod 16 inserted inside, the top of which penetrates the top of the component 13. Each of the rods 16 has a lever 17 connected to its lower outer side. Each of the components 13 has a through groove 18 on its lower outer side, and the through grooves 18 are all T-shaped. Each of the rods 16 has a disc 19 fitted on its lower outer side within the inner cavity of the component 13. Each of the rods 16 has a spring 20 fitted between the disc 19 and the top of the inner cavity of the component 13.

[0032] This solution, by adding multiple sets of fan-shaped test tube racks 9, not only allows for simultaneous storage and retrieval of various samples, reducing handling time, but also avoids cross-contamination between different samples during storage and retrieval because each channel is independent, ensuring sample purity and quality. Furthermore, the highly elastic shielding cloths 12 connected to both sides of the fan-shaped test tube racks 9 extend and retract when pulled out, thus shielding the outlet of the first liquid nitrogen tank 1 when the racks are opened, preventing leakage of liquid nitrogen. This not only reduces the evaporation rate of liquid nitrogen and saves on liquid nitrogen costs, but also reduces the workload of frequent liquid nitrogen replenishment. Moreover, the added highly elastic shielding cloths 12 prevent operators from directly contacting liquid nitrogen when handling biological test tube samples, reducing the risk of liquid nitrogen frostbite and improving the safety and reliability of the equipment.

[0033] It is to be noted that, in the present document, relational terms such as first and second and the like can be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0034] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and alterations can be made therein without departing from the spirit and scope of the application, which is defined by the appended claims and their equivalents.

Claims

1. A liquid nitrogen apparatus for biological sample storage, characterized by, include: A first liquid nitrogen tank (1) is provided, and a second liquid nitrogen tank (2) is provided on the right side of the first liquid nitrogen tank (1). A nitrogen generator (3) is provided on the right side of the second liquid nitrogen tank (2). Pipes (4) are connected between the first liquid nitrogen tank (1), the second liquid nitrogen tank (2) and the nitrogen generator (3). A siphon (5) is installed on the top of the first liquid nitrogen tank (1). A connecting pipe (6) is installed on the top of the siphon (5) and the top of the second liquid nitrogen tank (2). A chiller (7) is installed at the bottom of the connecting pipe (6). A chiller unit (8) is installed between the chillers (7) through a pipe (4). A fan-shaped test tube rack (9) is inserted circumferentially inside the first liquid nitrogen tank (1). The inner cavity of the first liquid nitrogen tank (1) is connected to a support plate (11) at the lower part of the fan-shaped test tube rack (9). High elastic shielding cloth (12) is connected to both sides of the outside of the fan-shaped test tube rack (9). The outer ends of the high elastic shielding cloth (12) are connected to the inner wall of the first liquid nitrogen tank (1).

2. The liquid nitrogen apparatus for biological sample storage of claim 1, wherein: The number of the fan-shaped test tube racks (9) is 3-5 sets. Each fan-shaped test tube rack (9) has an arc plate (10) installed at its outer end. Each arc plate (10) has a handle installed in the middle of its outer side. Each fan-shaped test tube rack (9) has a test tube insertion hole inside.

3. The liquid nitrogen apparatus for biological sample storage of claim 2, wherein: The upper outer side of the first liquid nitrogen tank (1) is equipped with a fitting (13) at a position corresponding to the arc plate (10), and the upper outer side of the arc plate (10) is equipped with a sleeve (131) at a position corresponding to the fitting (13).

4. The liquid nitrogen apparatus for biological sample storage of claim 3, wherein: Each of the mounting sets (13) has a plug rod (16) inserted inside, the top of each plug rod (16) penetrates the top of the mounting set (13), and a lever (17) is connected to the lower outer side of each plug rod (16).

5. The liquid nitrogen apparatus for biological sample storage of claim 4, wherein: Each of the outer lower sides of the assembly (13) is provided with a through groove (18), and the through groove (18) is T-shaped. The outer side of the insertion rod (16) is provided with a disc (19) located on the lower side of the inner cavity of the assembly (13). The outer side of the insertion rod (16) is provided with a spring (20) located between the disc (19) and the top of the inner cavity of the assembly (13).

6. The liquid nitrogen apparatus for biological sample storage of claim 1, wherein: The support plate (11) has a groove (14) in the middle of its interior, and the fan-shaped test tube rack (9) has a slider (15) installed in the middle of its bottom. The slider (15) is inserted into the groove (14), and the groove (14) and the slider (15) are both trapezoidal in shape.

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