Kit for detecting expression condition of antigen on plasma cell tumor

Abstract

The kit comprises an outer box body and an inner module, the outer box body is provided with a sample chamber and a heat preservation chamber from top to bottom, the bottom of the sample chamber is provided with a plurality of sample storage holes, the inner module is detachably nested in a hollow area of the sample chamber, and the heat preservation chamber is provided with a plurality of heat preservation holes. The inner module comprises an antibody module and a universal reagent module which are detachably connected in the horizontal direction, a plurality of antibody storage holes are formed in the antibody module, a plurality of universal reagent storage holes are formed in the universal reagent module, and box covers are further connected to the two adjacent side faces of the non-opening of the heat preservation cavity correspondingly; and the box cover can seal the hollow area of the sample chamber in an openable manner, wherein the inner module is detachably nested in the hollow area. According to the kit for detecting the expression condition of the antigen on the plasma cell tumor, an antibody cannot be taken wrongly, the stability and integrity of storage and taking of the kit are good, a light-proof environment is provided, and storage of a temperature-sensitive sample is ensured.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of biological detection instrument relates to a kit for detecting the expression of antigen on plasma cell tumor, specifically relates to a kit for detecting the expression of BCMA, MICA and MICB on plasma cell tumor. BACKGROUND

[0002] Multiple myeloma (MM) is a malignant plasma cell hematological tumor. Its tumor cells originate from plasma cells in bone marrow, and plasma cells, also known as effector B cells and antibody-secreting cells, are terminal differentiated cells differentiated from B cells under antigen stimulation. WHO classifies it as a kind of B cell lymphoma, called plasma cell myeloma / plasma cell tumor. B cell maturation antigen (BCMA) or CD269, also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF-17), has high level of restricted expression in normal and malignant plasma cells (PC), and is currently a popular ideal target antigen for new MM treatment. In a mouse model, BCMA overexpression can significantly promote the in vivo growth of xenotransplant MM cells, and in addition, studies have shown that the higher the BCMA level, the worse the prognosis, indicating that BCMA is a useful biomarker for MM disease activity and prognosis.

[0003] MM is characterized by a highly immunosuppressive bone marrow microenvironment, impaired anti-cancer / cytotoxic activity of natural killer (NK) cells, and loss of NKG2D ligands (MICA and MICB) in tumor cells, resulting in immune escape. NK cells play an important role in the immune surveillance of multiple myeloma (MM) progression, and their activity can be enhanced by combination therapy that can regulate the expression of specific activating ligands. Therefore, increased expression of MICA and MICB can promote NK cell-mediated cytotoxicity.

[0004] Multi-parameter flow cytometry is a technique for rapid multi-parameter analysis of individual cells or molecules in solution. Flow cytometry uses laser as light source to produce scattering light and fluorescence signal, which is read by photodiode or photomultiplier tube detector. It is widely used in clinical and scientific immunological status evaluation, immunotherapy efficacy monitoring, etc.

[0005] The existing plasma cell tumor detection kit has the following problems: multiple types of antibodies, inconvenient to take and distinguish; complex residual reagent metering procedure; unable to provide good test conditions simultaneously, such as providing independent light-proof devices required for incubation. UTILITY MODEL CONTENT

[0006] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide a reagent kit for detecting the expression of antigens on plasma cell tumors, which can make the reagents easy to handle, clearly distinguish the reagents, and make the measurement of remaining reagents simple, and can provide good experimental conditions.

[0007] To achieve the above and other related objectives, this utility model provides a reagent kit for detecting antigen expression on plasma cell tumors, comprising an outer box and an inner module. The outer box has a sample chamber and an insulated chamber arranged from top to bottom. The sample chamber is hollow and open at the top, and has several sample storage holes at the bottom. The insulated chamber is hollow and open on one side. The inner module is detachably nested within the hollow area of ​​the sample chamber. The inner module includes an antibody module and a universal reagent module detachably connected in the horizontal direction. The antibody module has several antibody storage holes, and the universal reagent module has several universal reagent storage holes. A lid is also connected to the adjacent non-opening sides of the insulated chamber, and the lid can be opened to close the hollow area of ​​the sample chamber where the inner module is detachably nested.

[0008] Preferably, the sample storage hole extends through the bottom of the sample chamber and communicates with the heat preservation chamber.

[0009] Preferably, the insulated cavity contains insulated material.

[0010] Preferably, the vertical height of the inner module is not less than the depth of the sample chamber.

[0011] Preferably, the antibody module and the general reagent module are respectively provided with a first connector and a second connector on the side where they contact each other, and the first connector and the second connector are detachably connected.

[0012] Preferably, the antibody module has fillable strips on its side.

[0013] More preferably, the fillable strip is disposed on the side not connected to the antibody module and the universal reagent module.

[0014] Preferably, the universal reagent module has a barcode strip on its side.

[0015] More preferably, the barcode strip is located on the side that is not the connection between the general reagent module and the antibody module.

[0016] Preferably, the box cover includes a first box cover and a second box cover; the first box cover includes a first cover segment, a second cover segment, and a third cover segment connected in sequence and foldable, the first cover segment being connected to the opposite side of the opening side of the heat preservation chamber, and the second cover segment being able to open and close the hollow area of ​​the sample chamber containing the detachable inner module, and the third cover segment being able to open and close the opening side of the heat preservation chamber; the second box cover includes a fourth cover segment, a fifth cover segment, and a sixth cover segment connected in sequence and foldable, the fourth cover segment being connected to the adjacent side of the opening side of the heat preservation chamber, and the fifth cover segment being able to open and close the hollow area of ​​the sample chamber containing the detachable inner module, and the sixth cover segment being able to open and close the opposite side of the heat preservation chamber connected to the fourth cover segment.

[0017] More preferably, a third connector is provided on the side of the third cover section facing the outer box, and a fourth connector is provided on the same side as the opening of the sample chamber and the insulation chamber, and the third connector and the fourth connector are detachably connected.

[0018] More preferably, a fifth connector is provided on the side of the sixth cover section facing the outer box body, and a sixth connector is provided on the sample chamber on the opposite side of the side where the heat preservation chamber connects to the fourth cover section, and the fifth connector and the sixth connector are detachably connected.

[0019] As described above, the kit for detecting antigen expression in plasma cell tumors provided by this invention has the following beneficial effects:

[0020] (1) The present invention provides a kit for detecting the expression of antigens on plasma cell tumors, which effectively improves the problems of existing kits, such as the large number of antibody types, inconvenient handling and differentiation, complex measurement procedures for remaining reagents, and inability to provide good experimental conditions at the same time, such as providing an independent light-proof device for incubation. It has the characteristics of being scientific, practical and convenient.

[0021] (2) The present invention provides a reagent kit for detecting the expression of antigens on plasma cell tumors. By setting up an antibody module and a universal reagent module, the reagents required for different test steps can be divided into two areas, realizing step-by-step reagent handling without manual searching, and also avoiding abnormal test results due to incorrect antibody handling.

[0022] (3) The present invention provides a kit for detecting the expression of antigens on plasma cell tumors. By setting a connector, the stability and integrity of the kit are facilitated in storage and handling.

[0023] (4) The present invention provides a reagent kit for detecting the expression of antigens on plasma cell tumors. A fillable strip is provided on the side of the antibody module for recording the number of times it is used. Considering the fixed number of times the reagent kit is used, this strip can be used for monitoring the remaining amount of reagents and inventory warning. A barcode strip is provided on the side of the general reagent module, which integrates the information of the reagent kit and the expiration date. It is used to connect to the reagent management software after scanning to realize the electronic file management of reagent information and status.

[0024] (5) The present invention provides a kit for detecting the expression of antigens on plasma cell tumors, which uses the outer box as a good independent antibody incubation device for the test, and solves the problem of the light-proof environment required for fluorescent antibodies by adding two tri-fold box lids and connectors.

[0025] (6) The present invention provides a kit for detecting the expression of antigens on plasma cell tumors. An insulated chamber is added to the outer box to hold the insulated material, so as to ensure that temperature-sensitive samples can also be taken into account. Attached Figure Description

[0026] Figure 1 The diagram shown is an overall structural schematic of a reagent kit for detecting antigen expression in plasma cell tumors according to the present invention, including the internal module structure.

[0027] Figure 2 The diagram shows the overall structure of a reagent kit for detecting antigen expression in plasma cell tumors according to this invention, including the outer casing.

[0028] Figure 3 This diagram shows the internal module structure of a reagent kit for detecting antigen expression in plasma cell tumors according to the present invention.

[0029] Figure 4 The diagram shows the outer casing of a reagent kit for detecting antigen expression in plasma cell tumors according to this invention.

[0030] Figure Labels

[0031] 1. Outer box

[0032] 11 Sample Chamber

[0033] 12 Insulated Chambers

[0034] 13 Sample storage wells

[0035] 14. Opening side

[0036] 2 Internal Modules

[0037] 21 Antibody Module

[0038] 22 General Reagent Module

[0039] 23 Antibody storage wells

[0040] 24 General reagent storage holes

[0041] 25 Fillable strips

[0042] 26 barcode strips

[0043] 3 box lids

[0044] 31 First box lid

[0045] 311 First Cover Section

[0046] 312 Second Cover Section

[0047] 313 Third Cover Section

[0048] 32 Second box lid

[0049] 321 Fourth Cover Section

[0050] 322 Fifth Cover Section

[0051] 323 Sixth Cover Section

[0052] 41 First Connector

[0053] 42 Second connector

[0054] 43 Third connector

[0055] 44 Fourth connector

[0056] 45 Fifth connector

[0057] 46 Sixth Connector Detailed Implementation

[0058] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0059] Please see Figures 1 to 4It should be understood that the structures, proportions, sizes, etc., illustrated in the accompanying drawings are merely for illustrative purposes to aid those skilled in the art and are not intended to limit the scope of this invention. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of this invention, should still fall within the scope of the disclosed technical content. Furthermore, the terms "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and not intended to limit the scope of this invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of this invention.

[0060] This invention provides a kit for detecting antigen expression in plasma cell tumors, such as... Figures 1-4 As shown, the device includes an outer box 1 and an inner module 2. The outer box 1 has a sample chamber 11 and an insulation chamber 12 from top to bottom. The sample chamber 11 is hollow and open at the top. The bottom of the sample chamber 11 has several sample storage holes 13. The insulation chamber 12 is hollow and open on one side. The inner module 2 is detachably nested in the hollow area of ​​the sample chamber 11. The inner module 2 includes an antibody module 21 and a universal reagent module 22 that are detachably connected in the horizontal direction. The antibody module 21 has several antibody storage holes 23, and the universal reagent module 22 has several universal reagent storage holes 24. A box cover 3 is also connected to the adjacent non-opening sides of the insulation chamber 12. The box cover 3 can be opened to close the hollow area of ​​the sample chamber 11 where the inner module 2 is detachably nested.

[0061] In the above kit, such as Figures 1-4 As shown, the sample storage hole 13 penetrates the bottom of the sample chamber 11 and communicates with the insulated chamber 12. It is used to securely hold test tubes containing samples, facilitating independent sample incubation. The sample is a pretreated, fresh leukocyte suspension.

[0062] In the above kit, such as Figure 1 , 2 As shown in Figure 4, the insulated chamber 12 contains insulating material. This material is used to keep the samples stored in the sample storage holes 13 of the sample chamber 11 warm, ensuring that samples with special temperature requirements can also be tested conveniently and accurately using this kit. The insulating material includes, but is not limited to, biological ice packs. The insulating material can be inserted and removed through the side opening of the insulated chamber 12.

[0063] In the above kit, such as Figure 1As shown, the vertical height of the inner module 2 is not less than the depth of the sample chamber 11.

[0064] In the above kit, such as Figure 3 As shown, the sides of the antibody module 21 and the universal reagent module 22 that are in contact with each other are respectively provided with a first connector 41 and a second connector 42, and the first connector 41 and the second connector 42 are detachably connected. This facilitates the handling and transfer of the antibody module 21 and the universal reagent module 22. For example, in the experimental stage, the antibody module 21 can be removed separately and stored in a light-proof environment.

[0065] In one embodiment, the detachable connection between the first connector 41 and the second connector 42 is a detachable magnetic connection. That is, both the first connector 41 and the second connector 42 are connectors containing magnets, and are connected by magnetic attraction between the magnets.

[0066] In the above kit, such as Figure 3 As shown, the antibody module 21 has fillable strips 25 on its side. These fillable strips 25 are tabular strips with borders, and the grids can be filled with color. They are used for recording and managing reagent usage. Specifically, one strip is filled for each person's dose of reagent used to record the antibody usage, facilitating reagent inventory management and early warning.

[0067] In one implementation, such as Figure 3 As shown, the fillable strip 25 is disposed on the side not connected to the antibody module 21 and the general reagent module 22.

[0068] In the above kit, such as Figure 3 As shown, a barcode strip 26 is provided on the side of the universal reagent module 22. The barcode strip 26 is a conventionally used, scannable barcode for managing reagent scanning information. Specifically, the barcode strip 26 integrates the reagent kit's inventory information and expiration date. After scanning, it connects to the reagent management software to achieve electronic record management of reagent information and status.

[0069] In one implementation, such as Figure 3 As shown, the barcode strip 26 is located on the side that is not the connection between the general reagent module 22 and the antibody module 21.

[0070] In the above kit, such as Figure 3 As shown, a reagent bottle for storing antibody reagents is snapped into the antibody storage hole 23. The antibody reagents include, but are not limited to, antibodies MICA / MICB, BCMA, CD138, CD38, CD56, CD19, CD45, etc.

[0071] In the above kit, such as Figure 3As shown, a reagent bottle for storing general reagents is snapped into the general reagent storage hole 24. The general reagents include, but are not limited to, hemolysin, PBS buffer, etc.

[0072] The aforementioned inner module 2 stores reagents through the antibody storage port 23 of the antibody module 21 and the universal reagent storage port 24 of the universal reagent module 22. This structure allows users to easily retrieve reagents according to experimental procedures, reducing the risk of reagent mix-ups, and flexibly storing antibody reagents that require proper light protection. Furthermore, during the addition of universal reagents or incubation, the antibody module 21 can be stored separately in a light-protected environment, ensuring the stability and integrity of reagent placement throughout the retrieval and storage process.

[0073] In the above kit, such as Figure 4 As shown, the box cover 3 includes a first box cover 31 and a second box cover 32; the first box cover 31 includes a first cover segment 311, a second cover segment 312, and a third cover segment 313 connected in sequence and foldable. The first cover segment 311 is connected to the opposite side of the opening side of the heat preservation chamber 12, and the second cover segment 312 can be opened to close the hollow area of ​​the sample chamber 11 where the inner module 2 is detachably nested. The third cover segment 313 can be opened to close the opening side of the heat preservation chamber 12; the second box cover 32 includes a fourth cover segment 321, a fifth cover segment 322, and a sixth cover segment 323 connected in sequence and foldable. The fourth cover segment 321 is connected to the adjacent side of the opening side of the heat preservation chamber 12, and the fifth cover segment 322 can be opened to close the hollow area of ​​the sample chamber 11 where the inner module 2 is detachably nested. The sixth cover segment 323 can be opened to close the opposite side of the heat preservation chamber 12 where the fourth cover segment 321 is connected. The first lid 31 and the second lid 32 mentioned above can form a cross lid, and when the outer box 1 is used alone, it can form an independent light-proof space required for antibody incubation.

[0074] In one implementation, such as Figure 4 As shown, the third cover section 313 is provided with a third connector 43 on the side facing the outer box 1, and the sample chamber 11 and the heat preservation chamber 12 are provided with a fourth connector 44 on the same side of their openings. The third connector 43 and the fourth connector 44 are detachably connected.

[0075] In a preferred embodiment, the detachable connection between the third connector 43 and the fourth connector 44 is a detachable magnetic connection. That is, both the third connector 43 and the fourth connector 44 are connectors containing magnets, and are connected by magnetic attraction between the magnets.

[0076] In one implementation, such as Figure 4As shown, a fifth connector 45 is provided on the side of the sixth cover section 323 facing the outer box 1, and a sixth connector 46 is provided on the sample chamber 11 on the same side as the side on which the heat preservation chamber 12 is connected to the fourth cover section 321. The fifth connector 45 and the sixth connector 46 are detachably connected.

[0077] In a preferred embodiment, the detachable connection between the fifth connector 45 and the sixth connector 46 is a detachable magnetic connection. That is, both the fifth connector 45 and the sixth connector 46 are connectors containing magnets, and are connected by magnetic attraction between the magnets.

[0078] The aforementioned lid 3, in conjunction with the outer box 1, secures the inner module 2, which is detachably nested within the hollow area of ​​the sample chamber 11, ensuring that all reagents remain stable and upright during reagent kit transportation. Simultaneously, the outer box 1 can also serve as a suitable and independent antibody incubation device for experiments. Furthermore, the tri-fold lid 3, connected to the adjacent non-opening sides of the insulated chamber 12, creates an independent, light-protected space for antibody incubation when the outer box 1 is used alone.

[0079] The following is combined Figures 1-4 This describes the specific usage process of a kit for detecting antigen expression in plasma cell tumors according to the present invention.

[0080] Operator obtains such Figures 1-4 When using a kit for detecting antigen expression on plasma cell tumors, the first lid 31 and the second lid 32 ensure that the reagents in the outer box 1 and inner module 2 remain stable and intact during storage and handling. By storing the kit as a whole and not mixing it with other antibodies, the instability of the results caused by reagent issues can be effectively avoided.

[0081] Open the first and second cover 31 and 32 of the cross-over box to detach the third connector 43 from the fourth connector 44 and the fifth connector 45 from the sixth connector 46. Remove the inner module 2 from the sample chamber 11 of the outer box 1. Following the experimental steps, take out the corresponding reagents from the antibody storage well 23 of the antibody module 21 and the universal reagent storage well 24 of the universal reagent module 22 for the experiment. The specific operation is as follows: Separate the first connector 41 and the second connector 42 to separate the antibody module 21 and the universal reagent module 22. Place the antibody module 21 in the refrigerator for use in the next step. Take out the outer box 1 of the reagent kit and the universal reagent module 22 and place them on the work surface. During this step, verify the reagent status (expiration date, quality control status) on the barcode strip 26 of the universal reagent module 22 and record the number of uses on the refillable strip 25 with a marker (record the number of uses corresponding to the number of antibody doses required this time). Invert and mix the sample to be tested (prepared leukocyte suspension). Add PBS buffer from universal reagent module 22 to the sample tube until it is about 3 / 4 full. Centrifuge at 1500 rpm for 5 min, discard the supernatant, and repeat the process twice. Resuspend the cells in PBS buffer to 1 mL and set aside. Remove antibody module 21 from the refrigerator and take out the antibody reagent from reagent storage well 23. Prepare a flow cytometry tube and label it with the specimen number. Add single-person antibody to the flow cytometry tube in sequence, and add 100 μL of washed sample. Mix well and incubate for 15 min in the dark. After incubation, add 1 mL of hemolysin to the flow cytometry tube, mix well, and incubate again at room temperature in the dark for 10 min. The above steps use the incubation device of this kit for low-temperature incubation in the dark: place a low-temperature biological ice pack through the side opening of the incubation chamber 12 as insulation material. This protects the state of the samples and antibody activity in the sample storage well 13 and antibody storage well 23, ensuring the stability and accuracy of plasma cell-associated antigen expression levels. The prepared flow cytometry tubes are attached to the sample storage well 13. The first and second lids 31 and 32 are then overlapped on the sample chamber, forming a clean, light-proof, and low-temperature incubation environment with the sample chamber 11. This is achieved through the triple-fold structure of the first lid 31 (first section 311, second section 312, and third section 313) and the second lid 32 (fourth section 321, fifth section 322, and sixth section 323). Simultaneously, the third connector 43 is connected to the fourth connector 44, and the fifth connector 45 is connected to the sixth connector 46. This creates an independent, clean, light-proof, and low-temperature incubation environment. The first connector 41 and the second connector 42 of the reagent module 21 are then connected and stored together in a refrigerator. This ensures that the temperature and ease of reagent access are met, while also maintaining overall integrity and preventing mixing with other antibodies, thus avoiding instability in the results caused by reagent issues. After incubation, 5 drops of PBS buffer are added to the flow cytometry tubes and mixed well. The samples are then analyzed within 12 hours.

[0082] This kit contains antibody reagents BCMA and MICA / MICB corresponding to specific antigens of plasma cell tumor progression and immune status, as well as antibody reagents required for accurate plasma cell population differentiation. These antibodies are flexibly integrated through antibody module 21, and the stability of sample status and plasma cell tumor antigen expression is ensured through a low-temperature incubation environment. It can not only accurately distinguish plasma cells using flow cytometry software, but also simultaneously and accurately assess the expression of specific antigens. It can be used for disease progression and prognostic assessment of plasma cell tumors, as well as related research on immune status.

[0083] In summary, the kit provided by this invention for detecting antigen expression in plasma cell tumors avoids antibody misuse, exhibits good stability and integrity during storage and handling, provides a light-protected environment, and ensures the storage of temperature-sensitive samples. The above embodiments are merely illustrative of the principles and effects of this invention and are not intended to limit it. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this invention should still be covered by the claims of this invention.

Claims

1. A kit for detecting antigen expression in plasma cell tumors, characterized in that, The device includes an outer box (1) and an inner module (2). The outer box (1) has a sample chamber (11) and a heat preservation chamber (12) from top to bottom. The sample chamber (11) is hollow and open at the top. The bottom of the sample chamber (11) has several sample storage holes (13). The heat preservation chamber (12) is hollow and open on one side. The inner module (2) is detachably nested in the hollow area of ​​the sample chamber (11). The inner module (2) includes a horizontally oriented... The antibody module (21) and the universal reagent module (22) are detachably connected. The antibody module (21) is provided with several antibody storage holes (23), and the universal reagent module (22) is provided with several universal reagent storage holes (24). The adjacent non-opening sides of the heat preservation chamber (12) are respectively connected with a box cover (3). The box cover (3) can be opened to close the hollow area of ​​the sample chamber (11) which is detachably nested with the inner module (2).

2. The kit for detecting antigen expression in plasma cell tumors according to claim 1, characterized in that, The sample storage hole (13) penetrates the bottom of the sample chamber (11) and communicates with the heat preservation chamber (12).

3. The kit for detecting antigen expression in plasma cell tumors according to claim 1, characterized in that, The vertical height of the inner module (2) is not less than the depth of the sample chamber (11).

4. The kit for detecting antigen expression in plasma cell tumors according to claim 1, characterized in that, The insulation chamber (12) contains insulation material.

5. The kit for detecting antigen expression in plasma cell tumors according to claim 1, characterized in that, The antibody module (21) and the general reagent module (22) are respectively provided with a first connector (41) and a second connector (42) on the side where they contact each other, and the first connector (41) and the second connector (42) are detachably connected.

6. The kit for detecting antigen expression in plasma cell tumors according to claim 1, characterized in that, The antibody module (21) has fillable strips (25) on its side.

7. The kit for detecting antigen expression in plasma cell tumors according to claim 1, characterized in that, The universal reagent module (22) has a barcode strip (26) on its side.

8. The kit for detecting antigen expression in plasma cell tumors according to claim 1, characterized in that, The lid (3) includes a first lid (31) and a second lid (32); the first lid (31) includes a first lid section (311), a second lid section (312), and a third lid section (313) connected in sequence, the first lid section (311) is connected to the opposite side of the opening side of the heat preservation chamber (12), and the second lid section (312) can be opened to close the hollow area of ​​the sample chamber (11) where the inner module (2) is detachably nested, and the third lid section (313) can be opened to close the heat preservation chamber (12). The second cover (32) includes a foldable fourth cover section (321), a fifth cover section (322), and a sixth cover section (323) connected in sequence. The fourth cover section (321) is connected to the adjacent side of the opening side of the heat preservation chamber (12), and the fifth cover section (322) can be opened to close the hollow area of ​​the sample chamber (11) with the detachable nested inner module (2). The sixth cover section (323) can be opened to close the opposite side of the heat preservation chamber (12) and the side connected to the fourth cover section (321).

9. The kit for detecting antigen expression in plasma cell tumors according to claim 8, characterized in that, The third cover section (313) is provided with a third connector (43) on the side facing the outer box (1), and the sample chamber (11) and the heat preservation chamber (12) are provided with a fourth connector (44) on the same side. The third connector (43) and the fourth connector (44) are detachably connected.

10. The kit for detecting antigen expression in plasma cell tumors according to claim 8, characterized in that, The sixth cover section (323) is provided with a fifth connector (45) on the side facing the outer box (1). The sample chamber (11) on the same side as the side on which the heat preservation chamber (12) connects to the fourth cover section (321) is provided with a sixth connector (46). The fifth connector (45) and the sixth connector (46) are detachably connected.

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