Micro-fluidic chip and micro-fluidic device

Abstract

The utility model discloses a micro-fluidic chip and a micro-fluidic device.The micro-fluidic chip comprises a liquid drop generation structure, the liquid drop generation structure comprises an adjusting component, a first micro-channel and two second micro-channels, dispersed-phase fluid is input into the initial end of the first micro-channel, continuous-phase fluid is input into the initial ends of the two second micro-channels, and the adjusting component is connected with the first micro-channel; the two second micro-channels intersect with the first micro-channel from the two sides respectively, liquid drops are output and generated in the downstream of the intersection position of the first micro-channel and the two second micro-channels, and the adjusting component is used for adjusting the flow of continuous-phase fluid output by the second micro-channels so as to control the size of the generated liquid drops. The flow of the continuous-phase fluid output by each second microchannel is controlled through the adjusting part, so that the size of the liquid drops generated when the dispersed-phase fluid conveyed by the first microchannel and the continuous-phase fluid conveyed by the two second microchannels converge is controllable, the size of each liquid drop can be independently adjusted by using the liquid drop generation structure array, and the liquid drop generation efficiency is improved. The uniformity of the generated liquid drops is improved, so that the preparation requirement is met.

Description

Technical Field

[0001] This application relates to the field of microfluidic chip technology, and in particular to a microfluidic chip and a microfluidic device. Background Technology

[0002] The cross-shaped structure is a common structure for passive droplet generation, used in the preparation of droplets or particles in biochemical reactions. Sometimes the daily throughput requirement for droplet generation can reach tens or even hundreds of microliters, while for droplets of tens of micrometers, the daily output of a single passive structure is typically only a few to tens of microliters, far from meeting the demand. Related technologies include schemes to multiply the throughput by arraying these structures; however, because droplet generation is extremely sensitive to input pressure or flow distribution, the droplet generation results vary to some extent between different array structures. Utility Model Content

[0003] The purpose of this application is to at least solve one of the technical problems existing in the prior art. To this end, this application proposes a microfluidic chip that, by adjusting the flow rate or pressure of a continuous phase fluid distributed by a component, uses a droplet generation structure array to individually adjust the size of each droplet, thereby improving the uniformity of the generated droplets and meeting the preparation requirements.

[0004] This application also proposes a microfluidic device including the aforementioned microfluidic chip.

[0005] The microfluidic chip according to the first aspect of this application includes:

[0006] A droplet generating structure includes an adjustment component, a first microchannel, and two second microchannels. The beginning of the first microchannel is used to input a dispersed phase fluid, and the beginnings of the two second microchannels are used to input a continuous phase fluid. The two second microchannels intersect with the first microchannel from both sides. The downstream position of the intersection of the first microchannel and the two second microchannels is used to output generated droplets. The adjustment component is used to adjust the flow rate of the continuous phase fluid output by each second microchannel to control the droplet size generated by the droplet generating structure.

[0007] The microfluidic chip according to the first aspect of this application has at least the following beneficial effects: by adjusting the flow rate of the continuous phase fluid output by each second microchannel, the size of the droplets generated when the dispersed phase fluid transported by the first microchannel and the continuous phase fluid transported by the two second microchannels converge is controllable. Using this droplet generation structure array, the size of each droplet can be adjusted individually, thereby improving the uniformity of the generated droplets and meeting the preparation requirements.

[0008] According to some embodiments of this application, the beginnings of the two second microchannels converge, and the adjustment component is disposed upstream of the convergence position of the two second microchannels to simultaneously adjust the flow rate of the continuous phase fluid in each of the second microchannels.

[0009] According to some embodiments of this application, the microfluidic chip further includes a continuous phase channel and a branch channel. The continuous phase channel is used to input a continuous phase fluid, and the branch channel is respectively connected to the confluence of the beginnings of the continuous phase channel and the two second microchannels. The adjustment component is disposed on the branch channel.

[0010] According to some embodiments of this application, at least two droplet generating structures are provided, the number of branch channels corresponds to the number of droplet generating structures, and each of the adjustment components is used to individually control the droplet size generated by each droplet generating structure.

[0011] According to some embodiments of this application, the continuous phase flow channel has a continuous phase inlet for inputting continuous phase fluid, and a channel outlet for outputting droplet generation is located downstream of the intersection of the first microchannel and the two second microchannels. Along the continuous phase fluid flow direction from the continuous phase inlet to the channel outlet, the widths of the continuous phase flow channel, the branch flow channel, the two second microchannels, and the first microchannel increase progressively.

[0012] According to some embodiments of this application, the microfluidic chip further includes a dispersed phase inlet, the beginning of the first microchannel is connected to the dispersed phase inlet, and multiple droplet generation structures are provided, with each droplet generation structure arranged in an array with the dispersed phase inlet as a reference.

[0013] According to some embodiments of this application, the regulating component includes a microvalve structure, which regulates the flow rate of the continuous phase flow in the second microchannel by means of air pressure regulation.

[0014] According to some embodiments of this application, the microvalve structure includes an air chamber, a diaphragm, and a microvalve channel. The diaphragm is disposed between the air chamber and the microvalve channel, and the diaphragm adjusts the flow rate of the microvalve channel in response to pressure changes in the air chamber.

[0015] According to some embodiments of this application, two second microchannels symmetrically intersect the first microchannel from both sides perpendicularly with respect to the first microchannel, so as to generate droplets through the cross intersection.

[0016] A microfluidic device according to a second aspect of this application includes a microfluidic chip as described in a first aspect of this application.

[0017] It is easy to understand that the microfluidic device in the second aspect embodiment of this application has the same technical effects as the microfluidic chip in the first aspect embodiment, and therefore will not be described again.

[0018] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0019] The present application will be further described below with reference to the accompanying drawings and embodiments;

[0020] Figure 1 This is a schematic diagram of an embodiment of this application;

[0021] Figure 2 This is a schematic diagram of the microvalve structure in an embodiment of this application.

[0022] Figure label:

[0023] 1000 Droplet generation structure; 1100 Adjustment component; 1110 Microvalve structure; 1111 Gas chamber; 1112 Thin film; 1113 Microvalve flow channel; 1200 First microchannel; 1210 Channel outlet; 1300 Second microchannel; 1400 Branch flow channel;

[0024] 2000, Continuous phase flow channel; 2100, Continuous phase inlet;

[0025] 3000, Dispersed phase inlet. Detailed Implementation

[0026] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0027] In the description of this application, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0028] In the description of this application, "several" means one or more, "more than" means at least two, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is for the purpose of distinguishing technical features only and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0029] In the description of this application, unless otherwise expressly defined, terms such as "setup," "installation," and "connection" should be interpreted broadly. Those skilled in the art can reasonably determine the specific meaning of the above terms in this application after considering the specific content of the technical solution.

[0030] Reference Figures 1 to 2 The microfluidic chip of the first aspect of this application is used to realize the uniform preparation of high-throughput droplets with an array cross structure. The microfluidic chip includes a droplet generation structure 1000.

[0031] The droplet generation structure 1000 includes an adjustment component 1100, a first microchannel 1200, and two second microchannels 1300. The beginning of the first microchannel 1200 is used to input a dispersed phase fluid, and the beginnings of the two second microchannels 1300 are used to input a continuous phase fluid. The two second microchannels 1300 intersect with the first microchannel 1200 from both sides. The downstream of the intersection of the first microchannel 1200 with the two second microchannels 1300 is used to output generated droplets. The adjustment component 1100 is used to adjust the flow rate of the continuous phase fluid output by each second microchannel 1300 to control the size of the droplets generated by the droplet generation structure 1000.

[0032] It is understood that by adjusting the flow rate of the continuous phase fluid output by each of the second microchannels 1300, the size of the droplets generated when the dispersed phase fluid transported by the first microchannel 1200 and the continuous phase fluid transported by the two second microchannels 1300 converge is controllable. Using the droplet generation structure 1000 array, the size of each droplet can be adjusted individually, thereby improving the uniformity of the generated droplets and meeting the preparation requirements.

[0033] It should be noted that when the droplet generation structure 1000 is used alone, the flow resistance of the continuous phase fluid can be adjusted by the adjusting component 1100, thereby achieving controllable droplet generation size. When this droplet generation structure 1000 array is set up, each droplet generation structure 1000 includes the adjusting component 1100, thereby achieving the effect of controllable droplet generation size at each structure. In some embodiments, the droplet generation size of each droplet generation structure 1000 is controlled to a uniform state by adjusting the adjusting component 1100 to meet the requirements of high-throughput droplet generation.

[0034] In other embodiments, a system can be formed by setting up multiple microfluidic chips. By adjusting the adjustment component 1100 in each microfluidic chip, the droplet generation size of each microfluidic chip can be controlled, and the requirement for high-throughput droplet generation can be met. Furthermore, when multiple microfluidic chips are set up to form a system for droplet generation, at least some of the microfluidic chips can have multiple droplet generation structures 1000 arrayed within them, and high-throughput and size-controllable droplet generation can be achieved by controlling each adjustment component 1100.

[0035] Understandably, depending on different design requirements, the setting of the regulating component 1100 and the design of the flow channels of the continuous phase fluid and the dispersed phase fluid can be adaptively adjusted, provided that the regulating component 1100 can control the flow rate of the continuous phase fluid output by each second microchannel 1300 and achieve uniform distribution of the flow rate or pressure of each droplet generation structure 1000 in the array.

[0036] In some embodiments, the second microchannel 1300 includes a beginning end, an end end, and a transport section located between the beginning end and the end end. The two second microchannels 1300 are respectively on both sides of the first microchannel 1200, and the transport sections of the two second microchannels 1300 and the first microchannel 1200 are both straight microchannels and parallel to each other.

[0037] In some embodiments of this application, reference is made to Figure 1 The two second microchannels 1300 converge at their beginnings. An adjusting component 1100 is positioned upstream of the convergence point of the two second microchannels 1300 to simultaneously adjust the flow rate of the continuous phase fluid in each second microchannel 1300. It can be understood that the two second microchannels 1300 satisfy the passive droplet generation conditions of a cross-shaped structure. Microdroplets encapsulated by the continuous phase fluid are formed at the convergence point of the two second microchannels 1300 and the first microchannel 1200. The convergence of the beginnings of the two second microchannels 1300 and the convergence of the ends of the two second microchannels 1300 with the first microchannel 1200 creates a parallel connection between the two second microchannels 1300. At this point, the flow rate of the continuous phase fluid output from each second microchannel 1300 can be controlled by the adjusting component 1100 positioned upstream of the convergence point, thus achieving the effect of controlling the droplet generation size.

[0038] It should be understood that this adjustment component 1100 is part of the droplet generating structure 1000. Therefore, when the droplet generating structure 1000 array is set, even if the adjustment component 1100 is upstream of the intersection of the two second microchannels 1300 in the droplet generating structure 1000, it will not exceed the range of the droplet generating structure 1000. Therefore, after the droplet generating structure 1000 array is set, each adjustment component 1100 is controlled independently, that is, there will be no mutual interference.

[0039] In some embodiments of this application, reference is made to Figure 1 The microfluidic chip also includes a continuous phase channel 2000 and a branch channel 1400. The continuous phase channel 2000 is used to input the continuous phase fluid, and the branch channel 1400 connects the beginning of the continuous phase channel 2000 and the two second microchannels 1300 respectively. The adjustment component 1100 is disposed on the branch channel 1400. It can be understood that by setting the branch channel 1400 to connect the continuous phase channel 2000 and the two second microchannels 1300, the layout of the droplet generation structure 1000 is optimized, making the overall structural design more reasonable. This makes it less prone to errors in the high-throughput droplet generation of the droplet generation structure 1000 after arraying, and allows for better uniform distribution of flow or pressure in the cross array structure by adjusting the adjustment component 1100.

[0040] In some embodiments, one end of the branch channel 1400 is connected to the continuous phase channel 2000, and the other end of the branch channel 1400 is connected to the starting point intersection of two second microchannels 1300. The adjustment component 1100 is disposed between the two ends of the branch channel 1400.

[0041] It is understood that the droplet generation structures 1000 are arrayed on the microfluidic chip. In some embodiments of this application, at least two droplet generation structures 1000 are provided, the number of branch channels 1400 corresponds to the number of droplet generation structures 1000, and each adjustment component 1100 is used to individually control the droplet size generated by each droplet generation structure 1000. The flow resistance is adjusted individually for each array structure to ensure that each array structure has equal pressure or flow distribution, thereby achieving high-throughput uniform droplet generation.

[0042] In some embodiments, based on this principle, up to dozens of droplet generation structures 1000 can be arrayed to achieve high-throughput, uniform droplet generation.

[0043] In other embodiments, the microfluidic chip further includes a continuous phase channel 2000, with adjustment components 1100 respectively disposed on the two second microchannels 1300. By adjusting the parameters of the two adjustment components 1100, the flow rate or pressure of the continuous phase fluid in the two second microchannels 1300 is made consistent and flows synchronously to converge with the first microchannel 1200 to generate droplets, which also achieves the effect of high-throughput droplet generation with controllable size. In this embodiment, the design of the branch channel 1400 is omitted, and the beginnings of the two second microchannels 1300 are directly connected to the continuous phase channel 2000 to transport the continuous phase fluid. In this embodiment, the droplet generation structures 1000 are arrayed on the microfluidic chip, and each adjustment component 1100 can also be used to individually control the droplet size generated by each droplet generation structure 1000.

[0044] It is understood that the flow distribution differences among the droplet generation structures 1000 after arraying can be reduced by designing at least one of the flow channels or channels, thereby reducing the adjustment frequency or adjustment range of the adjustment component 1100 and improving the droplet preparation efficiency. In some embodiments of this application, the continuous phase flow channel 2000 has a continuous phase inlet 2100 for inputting continuous phase fluid, and a channel outlet 1210 for outputting generated droplets is located downstream of the intersection of the first microchannel 1200 and the two second microchannels 1300. Along the continuous phase fluid flow direction from the continuous phase inlet 2100 to the channel outlet 1210, the widths of the continuous phase flow channel 2000, the branch flow channel 1400, the two second microchannels 1300, and the first microchannel 1200 increase progressively.

[0045] It is understood that this application achieves the effect of consistent or controllable droplet size by controlling the flow resistance of the continuous phase fluid. Therefore, along the flow direction of the continuous phase fluid, the continuous phase fluid is input from the continuous phase inlet 2100, flows sequentially through the continuous phase channel 2000, the branch channel 1400, and the two second microchannels 1300, and reaches the intersection of the two second microchannels 1300 and the first microchannel 1200, where droplets are formed. The droplets are then transported by the first microchannel 1200 to achieve droplet output. By designing the channels and passages through which the continuous phase fluid flows as a structure with increasing width from upstream to downstream, the difference in flow distribution between array structures can be reduced without activating the regulating component 1100.

[0046] In some embodiments, the width of the flow channels and passages through which the continuous phase fluid flows can be designed to increase incrementally according to actual needs, such as by linear increments or by segmented increments.

[0047] In some embodiments of this application, reference is made to Figure 1 The microfluidic chip also includes a dispersed phase inlet 3000. The beginning of the first microchannel 1200 is connected to the dispersed phase inlet 3000. Multiple droplet generation structures 1000 are provided, and each droplet generation structure 1000 is arranged in an array with the dispersed phase inlet 3000 as the reference. It can be understood that the first microchannel 1200, as a component of the droplet generation structure 1000, does not have a regulating component 1100 for adjusting the flow rate. Therefore, the first microchannel 1200 can be directly connected to the dispersed phase inlet 3000 to receive the dispersed phase fluid. By arranging the droplet generation structures 1000 in an array with the dispersed phase inlet 3000 as the reference, the spatial distribution of the first microchannel 1200 is optimized, making the layout of the droplet generation structures 1000 more reasonable.

[0048] In some embodiments, the array arrangement can be adjusted according to actual needs, provided that the usage conditions are met, such as horizontal arrangement, vertical arrangement, or circumferential distribution. Furthermore, the number of droplet generating structures 1000 can be adaptively adjusted according to the above array arrangement, such as 2, 4, 6, 8, etc.

[0049] In some embodiments, four droplet generating structures 1000 are arrayed on the microfluidic chip. Each droplet generating structure 1000 is arranged in a circumferential array and distributed in a cross shape. A continuous phase flow channel 2000 is arranged in the structure formed by the array, such that one end of the continuous phase flow channel 2000 serves as a continuous phase inlet 2100 for inputting the continuous phase fluid, the other end of the continuous phase flow channel 2000 is connected to one of the droplet generating structures 1000, and the remaining droplet generating structures 1000 are connected to the position between the two ends of the continuous phase flow channel 2000.

[0050] In some embodiments, the continuous phase inlet 2100 can receive a continuous phase fluid input from the microfluidic device, and the dispersed phase inlet 3000 can receive a dispersed phase fluid input from the microfluidic device, thereby generating droplets using the droplet generation structure 1000. In other embodiments, a chamber or fluid pool can be provided on this microfluidic chip, and a continuous phase fluid and a dispersed phase fluid can be respectively placed in the chamber or fluid pool and correspondingly transported to the continuous phase inlet 2100 and the dispersed phase inlet 3000, which can also achieve the effect of generating droplets using the droplet generation structure 1000.

[0051] In some embodiments of this application, the regulating component 1100 includes a microvalve structure 1110, which regulates the flow rate of the continuous phase flow in the second microchannel 1300 by means of air pressure regulation. By setting the microvalve structure 1110 on the continuous phase fluid channel, the individual flow resistance of the continuous phase of each array of droplet generation structures 1000 can be adjusted by regulating the air pressure acting on the microvalve structure 1110.

[0052] In other embodiments, the regulating component 1100 may also adaptively employ micro-valve structures 1110 such as electric regulating or hydraulic regulating without affecting normal use functions, thereby achieving the regulating effect.

[0053] In some embodiments of this application, reference is made to Figure 2The microvalve structure 1110 includes a gas chamber 1111, a diaphragm 1112, and a microvalve channel 1113. The diaphragm 1112 is disposed between the gas chamber 1111 and the microvalve channel 1113. The diaphragm 1112 regulates the flow rate of the microvalve channel 1113 in response to pressure changes in the gas chamber 1111. It is understood that the continuous phase fluid flows within the microvalve channel 1113. The pressure inside the gas chamber 1111 is adjustable, and changes in pressure cause the diaphragm 1112 to move, thereby regulating the flow in the microvalve channel 1113. This achieves the effect of individually adjusting the flow resistance of the continuous phase fluid in each array of droplet generation structures 1000.

[0054] In some embodiments, without affecting normal use, the air chamber 1111 and the micro-valve flow channel 1113 can be arranged vertically or horizontally relative to each other, and their specific positions can be interchanged adaptively, thereby achieving the effect of flow regulation.

[0055] In some embodiments, the air chamber 1111 can be externally connected to a pressure regulating mechanism to achieve adjustable internal pressure. In some embodiments, the membrane 1112 is made of a flexible material such as PDMS or silicone.

[0056] In some embodiments of this application, reference is made to Figure 1 Two second microchannels 1300 are symmetrically positioned on both sides of the first microchannel 1200, perpendicularly intersecting it to generate droplets at the cross intersection. It can be understood that the two second microchannels 1300 and the first microchannel 1200 form a cross-shaped passive droplet generation structure 1000, which is used for droplet or particle preparation in biochemical reactions, utilizing flow focusing to form discrete droplets.

[0057] In some embodiments, the upstream and downstream relative directions of the second microchannel 1300 are defined by the flow direction of the continuous phase fluid, and the upstream and downstream relative directions of the first microchannel 1200 are defined by the flow direction of the dispersed phase fluid and the flow direction of the formed droplets.

[0058] In some embodiments, the continuous phase fluid is an oil phase, and the dispersed phase fluid is an aqueous phase. The aqueous phase is injected into the channel from the aqueous phase inlet, and the oil phase is injected from the oil phase inlet. The oil and aqueous phases converge at the cross-section of the droplet generation structure 1000 in the array to generate droplets. In some embodiments, the regulating component 1100 includes an oil phase resistance regulating valve. The resistance regulating valve is provided with a micro-valve structure 1110, which can adjust the pressure of the gas chamber 1111 to adjust the individual flow resistance of the oil phase in each array structure.

[0059] In some embodiments, the channel material of the droplet generation structure 1000 is a hard or soft material in the microfluidics field, such as DMS, silicone, COC, COP, PS, or PC.

[0060] It is understandable that when aqueous phase is injected into the channel from the aqueous phase inlet and oil phase is injected from the oil phase inlet, the oil and aqueous phases will converge at the crossroads of the array structure. Since the flow resistance of the oil and aqueous phases differs across the multiple array channels, the droplet generation results will inevitably vary. To address this, in some embodiments of this application, by adjusting the adjusting component 1100 in each array structure, the droplet generation results of each array structure can be individually controlled, ensuring that each array structure has equal pressure or flow distribution, achieving consistent droplet size at each structure, thereby realizing high-throughput droplet generation.

[0061] The microfluidic device of the second aspect of this application may be a detection device for a microfluidic chip, a sampling and transfer device for a microfluidic chip, or a microfluidic droplet generation device, etc. The microfluidic device includes the microfluidic chip of the first aspect of this application. Since this microfluidic chip can realize the uniform preparation of high-throughput droplets with an array cross structure, the working efficiency of the microfluidic device with this microfluidic chip will also be improved to a certain extent.

[0062] It is easy to understand that the microfluidic device in the second aspect embodiment of this application has the same technical effects as the microfluidic chip in the first aspect embodiment, and therefore will not be described again.

[0063] The technical features of the above embodiments can be combined arbitrarily. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0064] The embodiments of this application have been described in detail above with reference to the accompanying drawings. However, this application is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this application.

Claims

1. A microfluidic chip, characterized in that, include: A droplet generating structure includes an adjustment component, a first microchannel, and two second microchannels. The beginning of the first microchannel is used to input a dispersed phase fluid, and the beginnings of the two second microchannels are used to input a continuous phase fluid. The two second microchannels intersect with the first microchannel from both sides. The downstream position of the intersection of the first microchannel and the two second microchannels is used to output generated droplets. The adjustment component is used to adjust the flow rate of the continuous phase fluid output by each second microchannel to control the droplet size generated by the droplet generating structure.

2. The microfluidic chip according to claim 1, characterized in that: The beginnings of the two second microchannels meet, and the adjustment component is located upstream of the meeting point of the two second microchannels to simultaneously adjust the flow rate of the continuous phase fluid in each of the second microchannels.

3. The microfluidic chip according to claim 2, characterized in that: The microfluidic chip further includes a continuous phase channel and a branch channel. The continuous phase channel is used to input the continuous phase fluid, and the branch channel is connected to the beginning intersection of the continuous phase channel and the two second microchannels respectively. The adjustment component is disposed on the branch channel.

4. The microfluidic chip according to claim 3, characterized in that: The droplet generating structure is provided in at least two, the number of branch channels corresponds to the number of droplet generating structures, and each of the adjustment components is used to individually control the size of the droplets generated by each droplet generating structure.

5. The microfluidic chip according to claim 3, characterized in that: The continuous phase flow channel has a continuous phase inlet for inputting continuous phase fluid, and a channel outlet for outputting droplet generation is located downstream of the intersection of the first microchannel and the two second microchannels. Along the continuous phase fluid flow direction from the continuous phase inlet to the channel outlet, the widths of the continuous phase flow channel, the branch flow channel, the two second microchannels, and the first microchannel increase progressively.

6. The microfluidic chip according to claim 2, characterized in that: The microfluidic chip also includes a dispersed phase inlet, the beginning of the first microchannel is connected to the dispersed phase inlet, and multiple droplet generation structures are provided, each droplet generation structure is arranged in an array with the dispersed phase inlet as a reference.

7. The microfluidic chip according to claim 1, characterized in that: The regulating component includes a microvalve structure, which regulates the flow rate of the continuous phase flow in the second microchannel by means of air pressure regulation.

8. The microfluidic chip according to claim 7, characterized in that: The microvalve structure includes an air chamber, a diaphragm, and a microvalve channel. The diaphragm is disposed between the air chamber and the microvalve channel, and the diaphragm adjusts the flow rate of the microvalve channel in response to pressure changes in the air chamber.

9. The microfluidic chip according to claim 1, characterized in that: The two second microchannels intersect the first microchannel symmetrically from both sides with respect to the first microchannel, so as to generate droplets through the cross intersection.

10. A microfluidic device, characterized in that, include: The microfluidic chip as described in any one of claims 1 to 9.

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