Cryopreservation device for cell exosome
By designing an exosome cryopreservation device with individual sealed storage and all-round sterilization functions, the problem of easy mixing and contamination of exosomes in the existing technology has been solved, and efficient and safe exosome storage has been achieved.
Patent Information
- Application Number
- CN202520086480.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-15
- Publication Date
- 2026-02-03
- Estimated Expiration
- 2035-01-15
AI Technical Summary
Existing extracellular oosome storage devices lack individual storage capabilities, leading to easy mixing of extracellular oosomes from different sources, increasing the risk of cross-contamination. Furthermore, repeated extractions can easily cause device contamination and extracellular oosome degradation, affecting the accuracy of experimental results and clinical application outcomes.
A cryopreservation device comprising an outer shell, storage chamber, sliding parts, storage tubes, and a lid has been designed. It features individual sealed storage and is equipped with a power supply, ultraviolet lamp, cooling unit, and display to achieve stable power supply, all-round sterilization, and efficient refrigeration, ensuring the pure storage of cell exosomes.
This technology enables the individual, sealed cryopreservation of exosomes, avoiding cross-contamination, ensuring sample purity, reducing the risk of contamination, and improving the accuracy and effectiveness of experimental and clinical applications.
Smart Images
Figure CN223860054U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell exosome cryopreservation technology, and in particular to a cell exosome cryopreservation device. Background Technology
[0002] Exosomes are a type of membrane vesicle secreted by cells, with diameters generally between 30 and 1000 nanometers. In recent years, with the progress of biomedical research, exosomes have attracted much attention due to their important role in intercellular communication, substance transport, and signal transduction. Exosomes can be produced by various cell types, including but not limited to tumor cells, immune cells, and neurons.
[0003] Existing devices mostly store exosomes through collective cryopreservation using support frames, without the ability to store them individually. Collective storage may lead to the mixing of exosomes from different sources, increasing the risk of cross-contamination and affecting the accuracy of experimental results. Furthermore, repeated extraction of exosomes by users can cause contamination inside the device, leading to the degradation and loss of function of the exosomes, directly impacting their effectiveness in biological research or clinical applications. Utility Model Content
[0004] The main objective of this invention is to provide a cryopreservation device for cell exosomes, which can effectively solve the problems in the background art.
[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows:
[0006] A cryopreservation device for exosomes includes an outer shell, a storage chamber rotatably connected to one side of the inner surface of the outer shell, a storage groove formed on one side of the inner surface of the storage chamber, and a sliding member slidably connected to one side of the inner wall of the storage chamber; a storage tube is fixedly connected to one side of the inner surface of the sliding member, and a cover is fixedly connected to one side of the outer surface of the sliding member, the cover being completely fitted to the outer surface of the storage chamber.
[0007] In order to provide power, as a cryopreservation device for exosomes according to this invention, a first support plate is fixedly connected to one side of the inner surface of the shell, a power source is fixedly connected to one side of the upper surface of the first support plate, and an electric wire is fixedly connected to one end of the power source.
[0008] To achieve a disinfection effect, as a cryopreservation device for exosomes according to this utility model, an ultraviolet lamp is fixedly connected to one side of the inner wall of the first support plate, a protective shell is fixedly connected to one side of the inner wall of the first support plate, and one end of the ultraviolet lamp is fixedly connected to an electric wire.
[0009] To enable cooling, as a cryopreservation device for exosomes according to this invention, a cooling body is fixedly connected to one side of the upper surface of the outer shell. The output end of the cooling body penetrates one side of the upper surface of the outer shell and extends to one side of the inner wall of the cooling body. A condenser is fixedly connected to the output end of the cooling body. The cooling body includes an evaporator and a compressor.
[0010] In order to promote the effect of heat exchange, as a cryopreservation device for exosomes of this invention, a condenser tube is fixedly installed on one side of the outer surface of the condenser, and an aluminum fin is fixedly connected to one end of the condenser tube.
[0011] In order to provide support and easy handling, as a cryopreservation device for exosomes according to this utility model, a support foot is fixedly connected to one side of the lower surface of the shell, and a door is rotatably connected to one side of the outer surface of the shell.
[0012] For the purpose of display, as a cryopreservation device for exosomes according to this invention, a display is fixedly connected to one side of the outer surface of the outer shell.
[0013] Compared with the prior art, the present invention has the following beneficial effects:
[0014] 1. In this utility model, through the arrangement of the outer shell, storage chamber, sliding member, storage tube and lid, in use, the user places the cell exosomes in the test tube, pushes the sliding member to open it, puts the test tube into the storage tube, pushes the lid, the sliding member slides, and closes the lid. The lid fits completely with the storage chamber, providing sealed protection, avoiding the influence of the external environment on the sample, and achieving the purpose of individual sealed cryopreservation.
[0015] 2. In this utility model, by setting up a power supply, wires and ultraviolet lamps, when the user freezes exosomes, by turning on the power supply, the wires transmit electrical energy to the ultraviolet lamps, providing stable and continuous power to the ultraviolet lamps, effectively killing bacteria and microorganisms on the outer surface of the storage chamber, and by fixing them to both sides of the device, comprehensive disinfection is achieved. Attached Figure Description
[0016] Figure 1 This is a schematic diagram of the main structure of an embodiment of the present utility model;
[0017] Figure 2 This is a schematic diagram of the door structure according to an embodiment of the present utility model;
[0018] Figure 3 This is a schematic diagram of the sliding component structure according to an embodiment of the present utility model;
[0019] Figure 4 This is a schematic diagram of the cooling main structure according to an embodiment of the present utility model;
[0020] Figure 5 This is a schematic diagram of the ultraviolet lamp structure according to an embodiment of the present utility model;
[0021] Figure 6 This is a schematic diagram of the storage compartment structure according to an embodiment of the present utility model.
[0022] In the diagram: 1. Outer shell; 2. Storage compartment; 3. Sliding component; 4. Storage tube; 5. Cover; 6. First support plate; 7. Power supply; 8. Wire; 9. Ultraviolet lamp; 10. Cooling unit; 11. Condenser; 12. Condenser tube; 13. Aluminum fins; 14. Support leg; 15. Door; 16. Display. Detailed Implementation
[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0024] Example 1
[0025] like Figure 1-6 As shown, a cryopreservation device for cell exosomes includes an outer shell 1, a storage chamber 2 rotatably connected to one side of the inner surface of the outer shell 1, a storage groove opened on one side of the inner surface of the storage chamber 2, and a sliding member 3 slidably connected to one side of the inner wall of the storage chamber 2.
[0026] In this embodiment, a storage tube 4 is fixedly connected to one side of the inner surface of the slider 3, and a cover 5 is fixedly connected to one side of the outer surface of the slider 3. The cover 5 is completely fitted to the outer surface of the storage compartment 2.
[0027] In practical use, the user places the exosomes in a test tube, pushes the slider 3 to open it, places the test tube into the storage tube 4, pushes the lid 5, the slider 3 slides, and the lid 5 closes, achieving the purpose of individual sealed cryopreservation.
[0028] In this embodiment, a first support plate 6 is fixedly connected to one side of the inner surface of the outer shell 1, a power supply 7 is fixedly connected to one side of the upper surface of the first support plate 6, and a wire 8 is fixedly connected to one end of the power supply 7.
[0029] In actual use, by turning on the power supply 7, electrical energy is transmitted to the device through the wire 8, providing a stable and continuous power supply.
[0030] In this embodiment, an ultraviolet lamp 9 is fixedly connected to one side of the inner wall of the first support plate 6, and a protective shell is fixedly connected to one side of the inner wall of the first support plate 6. One end of the ultraviolet lamp 9 is fixedly connected to the wire 8.
[0031] In actual use, the wire 8 transmits electrical energy to the ultraviolet lamp 9, providing a stable and continuous power to the ultraviolet lamp 9, effectively killing bacteria and microorganisms on the outer surface of the storage compartment 2, and achieving full coverage disinfection by fixing it to both sides of the device.
[0032] In this embodiment, a cooling body 10 is fixedly connected to one side of the upper surface of the outer casing 1. The output end of the cooling body 10 passes through one side of the upper surface of the outer casing 1 and extends to one side of the inner wall of the cooling body 10. A condenser 11 is fixedly connected to the output end of the cooling body 10. The cooling body 10 includes an evaporator and a compressor.
[0033] In practical use, heat is absorbed through the circulation of refrigerant inside the cooling body 10. When the gaseous refrigerant flows through the evaporator, the evaporator absorbs heat from the surrounding environment, causing the refrigerant to evaporate into gas. After the condenser 11 receives the high-pressure refrigerant gas from the cooling body 10, it helps the refrigerant release heat inside the condenser 11 and turn into a liquid state, thus achieving the cooling effect.
[0034] In this embodiment, a condenser tube 12 is fixedly installed on one side of the outer surface of the condenser 11, and an aluminum fin 13 is fixedly connected to one end of the condenser tube 12.
[0035] In practical use, the condenser tube 12 is connected to the condenser 11 to guide the coolant or condensate. The aluminum fins 13 are fixed to one end of the condenser tube 12 to provide additional surface area and promote more efficient heat exchange.
[0036] In this embodiment, a support foot 14 is fixedly connected to one side of the lower surface of the outer shell 1, and a door 15 is rotatably connected to one side of the outer surface of the outer shell 1.
[0037] In practical use, the support feet 14 are fixed to the lower surface of the outer shell 1 to provide a stable support base for the purpose of facilitating the user to pick up items.
[0038] In this embodiment, a display 16 is fixedly connected to one side of the outer surface of the housing 1.
[0039] In practical use, the display 16 is responsible for showing the cell's status, as well as the cooling temperature and time, so that the user can monitor and operate it.
[0040] Working principle: In use, the user places the exosomes in the test tube, pushes the slider 3 to open it, places the test tube into the storage tube 4, and pushes the lid 5, causing the slider 3 to slide and close the lid 5, achieving individual sealed cryopreservation. The power supply 7 is activated, and the wire 8 transmits electrical energy to the device, providing a stable and continuous power supply. The wire 8 also transmits electrical energy to the ultraviolet lamp 9, providing a stable and continuous power supply to the ultraviolet lamp 9, effectively killing bacteria and microorganisms on the outer surface of the storage chamber 2. By fixing it to both sides of the device, comprehensive disinfection is achieved. Heat is absorbed through the circulation of the internal refrigerant in the cooling body 10. When the gaseous refrigerant flows through the evaporator, the evaporator absorbs heat from the surrounding environment, causing the refrigerant to evaporate into a gas. After receiving the high-pressure refrigerant gas from the cooling body 10, the condenser 11 releases heat inside the condenser 11 and transforms the refrigerant into a liquid state, achieving a cooling effect. The condenser tube 12 is connected to the condenser 11 and is used to guide the coolant or condensate. It is fixed to one end of the condenser tube 12 by aluminum fins 13, providing additional surface area to promote more efficient heat exchange. The display 16 is responsible for displaying the cell status, as well as the cooling temperature and time, so that the user can monitor and operate to complete the cell storage.
[0041] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.
Claims
1. A cryopreservation device for exosomes, comprising a shell (1), characterized in that: A storage compartment (2) is rotatably connected to one side of the inner surface of the outer shell (1). A storage slot is provided on one side of the inner surface of the storage compartment (2). A sliding member (3) is slidably connected to one side of the inner wall of the storage compartment (2). A storage tube (4) is fixedly connected to one side of the inner surface of the sliding member (3). A cover (5) is fixedly connected to one side of the outer surface of the sliding member (3). The cover (5) is completely fitted to the outer surface of the storage compartment (2).
2. The cryopreservation device for exosomes according to claim 1, characterized in that: A first support plate (6) is fixedly connected to one side of the inner surface of the outer shell (1), and a power supply (7) is fixedly connected to one side of the upper surface of the first support plate (6). One end of the power supply (7) is fixedly connected to a wire (8).
3. The cryopreservation device for exosomes according to claim 2, characterized in that: An ultraviolet lamp (9) is fixedly connected to one side of the inner wall of the first support plate (6), and a protective shell is fixedly connected to one side of the inner wall of the first support plate (6). One end of the ultraviolet lamp (9) is fixedly connected to the wire (8).
4. The cryopreservation device for exosomes according to claim 1, characterized in that: A cooling body (10) is fixedly connected to one side of the upper surface of the outer shell (1). The output end of the cooling body (10) passes through one side of the upper surface of the outer shell (1) and extends to one side of the inner wall of the cooling body (10). A condenser (11) is fixedly connected to the output end of the cooling body (10). The cooling body (10) includes an evaporator and a compressor.
5. The cryopreservation device for exosomes according to claim 4, characterized in that: A condenser tube (12) is fixedly installed on one side of the outer surface of the condenser (11), and an aluminum fin (13) is fixedly connected to one end of the condenser tube (12).
6. The cryopreservation device for exosomes according to claim 1, characterized in that: A support foot (14) is fixedly connected to one side of the lower surface of the outer shell (1), and a door (15) is rotatably connected to one side of the outer surface of the outer shell (1).
7. The cryopreservation device for exosomes according to claim 1, characterized in that: A display (16) is fixedly connected to one side of the outer surface of the housing (1).