Multi-source mass spectrum sample testing system

Abstract

The utility model belongs to the field of sample analysis, and provides a multi-source mass spectrum sample testing system which comprises an online pretreatment unit, a dynamic biological evaluation unit and a multi-source chromatography-mass spectrum analysis unit, the online pretreatment unit comprises at least two parallel extraction branches, and all the extraction branches are connected with the high-speed counter-current chromatograph through a first switching valve; the high-speed counter-current chromatograph is respectively connected with the dynamic biological evaluation unit and the multi-source chromatography-mass spectrometry analysis unit through a second switching valve; the dynamic biological evaluation unit comprises a multi-channel injection pump module, a micro-fluidic chip / organ chip cell culture module and a micro solid-phase extraction module; the input end of the multi-channel injection pump module is respectively connected with the high-speed counter-current chromatograph and the cell culture fluid vessel, and the output end of the multi-channel injection pump module is connected with the micro-fluidic chip / organ chip cell culture module; the input end of the micro solid-phase extraction module is connected with the micro-fluidic chip / organ chip cell culture module, and the output end of the micro solid-phase extraction module is connected with the multi-source chromatography-mass spectrometry unit.

Description

Technical Field

[0001] This utility model belongs to the field of sample analysis, and in particular relates to a multi-source mass spectrometry sample testing system. Background Technology

[0002] The statements in this section are merely background information related to this utility model and do not necessarily constitute prior art.

[0003] In the process of identifying the material composition and bioactivity-toxicity effects of samples, as well as in practical applications, the study typically includes multi-component separation and analysis within the sample, bioevaluation, and analysis and identification. In the separation and analysis stage, traditional separation and analysis devices often employ offline step-by-step methods, resulting in complex and cumbersome operations, multiple offline steps, and high consumption of reagents and other resources. In the bioevaluation stage, current bioevaluation cell cultures primarily rely on conventional static cultures, which cannot effectively reflect the state of the cellular microenvironment within the organism. In the analysis and identification stage, commonly used online mass spectrometry systems primarily use single-source ion settings, requiring tedious system tuning and optimization for switching. Utility Model Content

[0004] To address the technical problems mentioned above, this invention provides a multi-source mass spectrometry sample testing system that is easy to operate and can meet the needs of online sample analysis.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] The first aspect of this utility model provides a multi-source mass spectrometry sample testing system.

[0007] A multi-source mass spectrometry sample testing system includes: an online preprocessing unit, a dynamic biological evaluation unit, and a multi-source chromatography-mass spectrometry analysis unit;

[0008] The online pretreatment unit includes at least two parallel extraction branches, all of which are connected to a high-speed countercurrent chromatograph via a first switching valve; the high-speed countercurrent chromatograph is connected to a dynamic biological evaluation unit and a multi-source chromatography-mass spectrometry analysis unit via a second switching valve.

[0009] The dynamic biological evaluation unit includes a multi-channel injection pump module, a microfluidic chip / organ-on-a-chip cell culture module, and a micro solid-phase extraction module. The input of the multi-channel injection pump module is connected to a high-speed countercurrent chromatograph and a cell culture medium vessel, respectively, and the output is connected to the microfluidic chip / organ-on-a-chip cell culture module. The input of the micro solid-phase extraction module is connected to the microfluidic chip / organ-on-a-chip cell culture module, and the output is connected to a multi-source chromatography-mass spectrometry analysis unit.

[0010] In one implementation, the dynamic biological evaluation unit further includes a microscope module, which is positioned above the microfluidic chip / organ-on-a-chip cell culture module for observing the condition of cells in the chip.

[0011] In one implementation, the dynamic biological evaluation unit further includes a CO2 control module, which is connected to the microfluidic chip / organ-on-a-chip cell culture module.

[0012] In one implementation, the dynamic biological evaluation unit further includes a temperature control module, which is connected to the microfluidic chip / organ-on-a-chip cell culture module.

[0013] In one implementation, the output of the dynamic biological evaluation unit is also synchronously connected to multiple sites on the target plate.

[0014] In one implementation, the multi-source chromatography-mass spectrometry analysis unit consists of an integrated liquid chromatograph and a high-resolution mass spectrometer.

[0015] As one implementation method, an automatic solid-phase extraction device is provided on the extraction branch.

[0016] In one implementation, an automatic magnetic solid-phase extraction device is provided on the extraction branch.

[0017] In one embodiment, a solvent extraction device is provided on the extraction branch.

[0018] In one implementation, the first switching valve is a multi-input single-output switching valve; the second switching valve is a single-input multi-output switching valve.

[0019] The beneficial effects of this utility model are:

[0020] This utility model's multi-source mass spectrometry sample testing system integrates three units: an online pretreatment unit, a dynamic biological evaluation unit, and a multi-source chromatography-mass spectrometry analysis unit. It utilizes the parallel extraction branch of the online pretreatment unit to extract different sample types, followed by dynamic biological evaluation of relevant components at the cellular level using the dynamic biological evaluation unit. Finally, the multi-source chromatography-mass spectrometry analysis unit enables the identification and quantitative analysis of various sample components and the detection of differentially expressed metabolites and proteins. The overall multi-source mass spectrometry sample testing system is easy to operate and meets the needs of real-world sample analysis online.

[0021] Advantages of the present invention in additional aspects will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0022] The accompanying drawings, which form part of this specification, are used to provide a further understanding of this utility model. The illustrative embodiments of this utility model and their descriptions are used to explain this utility model and do not constitute an improper limitation of this utility model.

[0023] Figure 1 This is a schematic diagram of the structural block of the online preprocessing and dynamic biological evaluation mass spectrometry analysis system of this utility model;

[0024] Figure 2 This is a schematic diagram of the dynamic biological evaluation module of this utility model;

[0025] Figure 3 This is a schematic diagram of the dynamic biological evaluation unit 2 of this utility model. Detailed Implementation

[0026] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0027] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0028] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to the present invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0029] In this utility model, terms such as "upper", "lower", "left", "right", "front", "back", "vertical", "horizontal", "side", and "bottom" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only used to facilitate the description of the structural relationship between the various components or elements of this utility model and do not specifically refer to any component or element in this utility model. They should not be construed as limiting this utility model.

[0030] In this utility model, terms such as "fixed connection," "connected," and "joined" should be interpreted broadly, indicating a fixed connection, an integral connection, or a detachable connection; a direct connection or an indirect connection through an intermediate medium. For those skilled in the art, the specific meaning of the above terms in this utility model can be determined according to the specific circumstances, and should not be construed as a limitation of this utility model.

[0031] like Figure 1As shown, this utility model provides a multi-source mass spectrometry sample testing system, including: an online preprocessing unit 1, a dynamic biological evaluation unit 2, and a multi-source chromatography-mass spectrometry analysis unit 3;

[0032] The online pretreatment unit 1 includes at least two parallel extraction branches 11, all of which are connected to a high-speed countercurrent chromatograph 13 via a first switching valve 12. The high-speed countercurrent chromatograph 13 is connected to the dynamic biological evaluation unit 2 and the multi-source chromatography-mass spectrometry analysis unit 3 via a second switching valve 14. Figure 2 As shown.

[0033] For example, an automatic solid-phase extraction device is installed on the extraction branch 11. An automatic magnetic solid-phase extraction device is installed on the extraction branch 11. A solvent extraction device is installed on the extraction branch 11.

[0034] In one or more embodiments, the first switching valve 12 is a multi-input single-output switching valve; the second switching valve 14 is a single-input multi-output switching valve.

[0035] The dynamic biological evaluation unit 2 includes a multi-channel injection pump module 21, a microfluidic chip / organ-on-a-chip cell culture module 22, and a micro solid-phase extraction module 23. The input of the multi-channel injection pump module 21 is connected to a high-speed countercurrent chromatograph and cell culture medium vessels, respectively, and its output is connected to the microfluidic chip / organ-on-a-chip cell culture module 22. The input of the micro solid-phase extraction module 23 is connected to the microfluidic chip / organ-on-a-chip cell culture module 22, and its output is connected to the multi-source chromatography-mass spectrometry analysis unit 3. The multi-source chromatography-mass spectrometry analysis unit 3 consists of an integrated liquid chromatograph 31 and a high-resolution mass spectrometer 32. The micro solid-phase extraction module 23 has a conventional structure, for example, including a sampler and a rinsing system.

[0036] In other embodiments, the dynamic biological evaluation unit further includes a microscope module 24, which is disposed above the microfluidic chip / organ-on-a-chip cell culture module 22 for observing the condition of cells in the chip.

[0037] The microfluidic chip / organ-on-a-chip cell culture module 22 here can also be an existing structure, which integrates a microfluidic chip and an organ-on-a-chip. Moreover, the microfluidic chip and organ-on-a-chip can be selected according to actual needs by those skilled in the art.

[0038] In other embodiments, the dynamic biological evaluation unit further includes a CO2 control module 25, which is connected to the microfluidic chip / organ-on-a-chip cell culture module 22.

[0039] The CO2 control module here is an existing structure, which is used to introduce a set amount of CO2 into the microfluidic chip / organ-on-a-chip cell culture module; the CO2 control module can be composed of a CO2 gas tank and an automatic valve connected to the outlet of the CO2 gas tank.

[0040] In other embodiments, the dynamic biological evaluation unit further includes a temperature control module, which is connected to the microfluidic chip / organ-on-a-chip cell culture module.

[0041] In one or more embodiments, the output of the dynamic biological evaluation unit is also synchronously connected to multiple sites on the target plate.

[0042] This invention provides an integrated device system for the analysis of compounds in complex systems and for biodynamic evaluation and activity-toxicity screening. It has strong application and promotion value in the analysis of complex samples, especially in the screening of active substances in natural products. The bioevaluation system utilizes microfluidics or organ-on-a-chip technology for dynamic cell culture, highly simulating the dynamic microenvironment in vivo. It integrates multiple functional units, allowing for real-time microscopic observation of cell status and dynamic extraction and measurement of metabolites. It offers advantages such as low reagent consumption, high speed, high throughput, and high sensitivity.

[0043] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A multi-source mass spectrometry sample testing system, characterized in that, include: Online preprocessing unit, dynamic biological evaluation unit, and multi-source chromatography-mass spectrometry analysis unit; The online pretreatment unit includes at least two parallel extraction branches, all of which are connected to a high-speed countercurrent chromatograph via a first switching valve; the high-speed countercurrent chromatograph is connected to a dynamic biological evaluation unit and a multi-source chromatography-mass spectrometry analysis unit via a second switching valve. The dynamic biological evaluation unit includes a multi-channel injection pump module, a microfluidic chip / organ-on-a-chip cell culture module, and a micro solid-phase extraction module. The input of the multi-channel injection pump module is connected to a high-speed countercurrent chromatograph and a cell culture medium vessel, respectively, and the output is connected to the microfluidic chip / organ-on-a-chip cell culture module. The input of the micro solid-phase extraction module is connected to the microfluidic chip / organ-on-a-chip cell culture module, and the output is connected to a multi-source chromatography-mass spectrometry analysis unit.

2. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, The dynamic biological evaluation unit also includes a microscope module, which is positioned above the microfluidic chip / organ-on-a-chip cell culture module to observe the condition of cells in the chip.

3. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, The dynamic biological evaluation unit also includes a CO2 control module, which is connected to the microfluidic chip / organ-on-a-chip cell culture module.

4. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, The dynamic biological evaluation unit also includes a temperature control module, which is connected to the microfluidic chip / organ-on-a-chip cell culture module.

5. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, The output of the dynamic biological evaluation unit is also synchronously connected to multiple sites on the target plate.

6. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, The multi-source chromatography-mass spectrometry analysis unit consists of an integrated liquid chromatograph and a high-resolution mass spectrometer.

7. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, An automatic solid-phase extraction device is installed on the extraction branch.

8. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, An automatic magnetic solid phase extraction device is installed on the extraction branch.

9. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, A solvent extraction device is installed on the extraction branch.

10. The multi-source mass spectrometry sample testing system as described in claim 1, characterized in that, The first switching valve is a multi-input single-output switching valve; the second switching valve is a single-input multi-output switching valve.

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