Multi-time blood sampling device for rats in waking state

Abstract

The utility model belongs to the technical field of experimental instruments, and discloses a repeated blood sampling device for a rat in a waking state, which comprises a three-way tube, and the three-way tube comprises an injection tube, an anticoagulation tube and a blood sampling tube; a control valve is arranged in the three-way pipe, communication between different pipelines is controlled by rotating the control valve, so that heparin injection and blood taking are conducted, a pressing button is arranged at the top of the control valve, a blood taking channel can be located between the injection pipe and the blood taking pipe by pressing the pressing button downwards, and therefore a small amount of blood can be taken, and the blood taking precision can be improved. The device is connected with an external jugular vein cannula of a laboratory mouse through an operation, so that blood can be repeatedly taken for multiple times when the laboratory mouse is in a sober state, and blood coagulation can be avoided; the utility model solves the problems that the blood drawing process is troublesome and the accuracy is insufficient in the prior art, and is suitable for the blood drawing measurement of laboratory mice.

Description

Technical Field

[0001] This utility model relates to the field of laboratory equipment technology, and in particular to a device for multiple blood collection from rats while they are awake. Background Technology

[0002] In animal experiments using mice as a model, blood tests are often used as the primary standard for assessing health status and evaluating pharmacokinetics; therefore, blood collection from mice is a common procedure. Currently, most common blood collection methods involve direct syringe extraction, but this requires multiple punctures when multiple blood draws are needed, which is cumbersome. Furthermore, the accuracy of the blood volume drawn during mouse blood collection is crucial. Therefore, this invention proposes a device for repeated blood collection from rats while they are awake, enabling repeated blood collection while the animal is conscious. Utility Model Content

[0003] The present invention aims to provide a device for multiple blood collection from rats while they are awake, in order to solve the problems of the cumbersome blood collection process and insufficient accuracy of the existing technology.

[0004] To achieve the above objectives, this utility model provides the following technical solution:

[0005] A device for multiple blood collection from rats in an conscious state includes a three-way valve with a control valve. The three-way valve includes an injection tube, an anticoagulant tube, and a blood collection tube. The injection tube is connected to a flexible tube. The bottom end of the control valve is rotatably connected to the inside of the three-way valve. The bottom end of the control valve has a T-shaped channel that is flush with the injection tube, anticoagulant tube, and blood collection tube. A push button is slidably connected to the center of the control valve. The push button passes through the control valve. A spring is located on the outside of the push button and is connected to the top of the control valve via the spring. A blood collection channel is located at the bottom of the push button, and the cross-sectional area of ​​the blood collection channel is smaller than that of the T-shaped channel.

[0006] Furthermore, a label is provided on the top of the control valve to indicate the connection direction of the T-channel.

[0007] Furthermore, sliders are connected to both sides of the button, and a groove is provided inside the control valve. The button and the control valve are slidably connected through the cooperation of the sliders and the groove.

[0008] Furthermore, the tubing is a 5ml syringe needle tube.

[0009] The principle and beneficial effects of the technical solution: The flexible tube of this utility model device is surgically connected to the external jugular vein of a laboratory mouse for venous cannulation. After suturing and fixation, blood can be drawn multiple times while the mouse is awake via the blood collection tube. During blood collection, rotating the control valve connects the blood collection tube to the injection tube, allowing for blood collection. Rotating the control valve also connects the anticoagulant tube to the injection tube, enabling the injection of heparin to prevent clotting. Simultaneously, depending on the amount of blood collected, pressing the button allows for precise control. When the amount of blood collected is small, pressing the button downwards connects the injection tube, blood collection channel, and blood collection tube. This results in a smaller and more accurate blood collection volume compared to when the button is not pressed, even at the same extraction distance. Attached Figure Description

[0010] Figure 1 This is a structural diagram of the present invention;

[0011] Figure 2 This is an exploded view of the present invention;

[0012] Figure 3 This is a cross-sectional view of the three-way valve during heparin injection according to this utility model;

[0013] Figure 4 This is a cross-sectional view of the three-way tube used for blood drawing according to this utility model;

[0014] Figure 5 This is a cross-sectional view of the three-way tube when the blood draw volume is small.

[0015] Figure 6 This is a front cross-sectional view of the three-way tube when the blood draw volume is small.

[0016] The corresponding labels in the attached diagram are named as follows: 1. Three-way valve; 2. Control valve; 3. Press button; 11. Injection tube; 12. Anticoagulant tube; 13. Blood collection tube; 14. Tube; 21. T-shaped channel; 22. Label; 23. Slide groove; 31. Blood collection channel; 32. Slider. Detailed Implementation

[0017] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments:

[0018] like Figures 1-5As shown, a device for multiple blood collection from rats in an conscious state includes a three-way valve, comprising an injection tube, an anticoagulant tube, and a blood collection tube. A flexible tube, which is a 5ml syringe needle tube, is connected to the front end of the injection tube. A control valve is located at the top of the three-way valve, and a T-shaped channel is located at the bottom of the control valve. The bottom of the control valve is rotatably connected inside the three-way valve. The T-shaped channel is horizontally aligned with the injection tube, anticoagulant tube, and blood collection tube. A push button is slidably connected to the center of the control valve, with sliders connected to both sides of the push button. A groove is provided inside the control valve, and the sliders slide within the groove, causing the push button to slidably connect to the control valve. The push button passes through the control valve. The top of the control valve is connected to the push button via a spring. A blood collection channel is located at the bottom of the push button, and the cross-sectional area of ​​the blood collection channel is smaller than the cross-sectional area of ​​the T-shaped channel.

[0019] The specific implementation process is as follows: First, the experimental mice are anesthetized, the surgical area is shaved, and the surgical area is disinfected; an incision is made in the surgical area, a subcutaneous tunnel is made by puncturing with a puncture needle, then the external jugular vein is bluntly dissected, a cannula is inserted into the external carotid artery, and finally the area is sutured and fixed to complete the surgery.

[0020] When heparin injection is required, the internal state of the three-way stopcock is as follows: Figure 3 As shown, the anticoagulant tubing is connected to the injection tubing, allowing heparin to be injected to prevent clotting; when blood needs to be drawn, the internal state of the three-way stopcock is as follows. Figure 4 As shown, the blood collection tube is connected to the injection tubing, allowing blood to be drawn from the collection tube. When a smaller amount of blood is needed, the internal state of the three-way stopcock is as follows. Figure 5 , 6 As shown, when the button is pressed down, a blood collection channel with a smaller cross-sectional area is formed between the blood vessel and the injection tube, which allows for the collection of less blood and greater precision.

[0021] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this utility model. These modifications and improvements should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A device for repeated blood sampling in a conscious rat, characterized in that: The utility model provides a control valve (2) is arranged to three -way pipe (1), three -way pipe (1) includes injection pipe (11), anticoagulation pipe (12) and blood taking pipe (13), injection pipe (11) is connected with hose (14), control valve (2) bottom end rotationally connected in three -way pipe (1) inside, control valve (2) bottom end is provided with T type channel (21), T type channel (21) with injection pipe (11), anticoagulation pipe (12) and blood taking pipe (13) level flush, the inside center of control valve (2) is slidably connected with press button (3), press button (3) is provided with spring outside, press button (3) is connected with control valve (2) top through spring, press button (3) bottom is provided with blood taking channel (31), and the cross -sectional area of blood taking channel (31) is less than the cross -sectional area of T type channel (21). ​ 2. The device for repeated blood sampling in conscious rats according to claim 1, characterized in that: Control valve (2) top is provided with mark (22), and the mark (22) is used to indicate the communication direction of T type channel (21).

3. The device for repeated blood sampling in conscious rats according to claim 1, characterized in that: The both sides of the press button (3) are connected with the sliding block (32), and the control valve (2) is provided with the sliding groove (23) inside, and the press button (3) is slidably connected with the control valve (2) by the cooperation of the sliding block (32) and the sliding groove (23).

4. The device for repeated blood sampling from a conscious rat according to claim 1, wherein: The hose (14) is 5ml syringe needle pipe.

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