Reagent storage mechanism of biological tissue sample section dyeing equipment

Abstract

The utility model provides a reagent storage mechanism of biological tissue sample section dyeing equipment, which comprises a clean water storage component, a clean water pumping component, a dyeing reagent storage component and a dyeing reagent pumping component, and a main console of the biological tissue sample section dyeing equipment is in control connection with the clean water pumping component and the dyeing reagent pumping component. The clean water storage assembly is communicated with the clean water pumping assembly, the clean water pumping assembly is matched with the dyeing tank cleaning assembly and the glass slide discharging cleaning assembly, the dyeing reagent storage assembly is communicated with the dyeing reagent pumping assembly, and the dyeing reagent pumping assembly is matched with the dyeing reagent temporary storage assembly. The dyeing reagent storage assembly comprises a plurality of dyeing reagent storage containers arranged in an array mode, and the dyeing reagent storage containers are communicated with the dyeing reagent pumping assembly. The dyeing reagent storage device has the beneficial effects that the dyeing reagent can be stored, and the dyeing reagent can be automatically added and supplemented into the dyeing reagent pumping assembly.

Description

Technical Field

[0001] This utility model relates to the field of biological tissue sample staining technology, specifically to a reagent storage mechanism for a biological tissue sample staining device. Background Technology

[0002] In existing biological sample analysis techniques, commonly used analytical methods include microscopy, microarray analysis (e.g., protein and nucleic acid microarray analysis), and mass spectrometry. Preparing samples for these and other types of analysis typically involves contacting the biological sample with a series of processing liquids. Some of these processing liquids (e.g., staining reagents and counterstaining reagents) can add color and, conversely, alter the visual properties of invisible or difficult-to-see sample components (e.g., at least some types of cells and intracellular structures). Other processing liquids (e.g., deparaffin liquids) can be used to achieve other processing purposes. If multiple processing liquids are used to treat the sample, the application and subsequent removal of each liquid can be crucial for producing a sample suitable for analysis. In some cases, treating samples with multiple processing liquids involves manually applying the liquids to the microscope slides holding the samples. However, this manual sample preparation method tends to be labor-intensive and inaccurate, leading to the development of biological tissue section staining equipment.

[0003] Automated staining technology for biological tissue samples is a crucial and indispensable step in the entire automated staining, drying, and mounting process for biological tissue samples. To ensure that the processed biological tissue samples have an easily observable appearance and maintain consistency and efficiency in the processing, the staining step is an essential aspect that must be strictly controlled during biological tissue sample processing. The purpose of staining is to make different structures within the cellular tissue appear in different colors for easier observation. The classic hematoxylin and eosin staining method is the routine staining method for histological and pathological specimens, abbreviated as HE staining. After staining, the cell nuclei are stained purple-blue by hematoxylin, while most cytoplasm and non-cellular components are stained pink by eosin.

[0004] Existing biological tissue sample staining equipment can replace manual staining. These machines automatically process samples by immersing a rack holding a batch of microscope slides in an open bath containing processing liquid. However, during the immersion staining operation, existing biological tissue sample staining equipment requires frequent replenishment of various staining reagents as they are consumed over time. This manual replenishment is cumbersome, time-consuming, labor-intensive, and prone to errors, resulting in inconsistent production efficiency and product quality that fails to meet user needs. Utility Model Content

[0005] To address the problems in the prior art, this utility model provides a reagent storage mechanism for a biological tissue sample section staining device. By arranging a water storage component, a water pumping component, a staining reagent storage component, and a staining reagent pumping component that work together within the reagent storage mechanism, it is possible to automatically add supplementary staining reagent to the staining reagent temporary storage component. The staining reagent in the temporary storage component can be automatically replenished to the staining tank without manual operation, saving time and effort and reducing errors. This solves the problem that manually adding supplementary staining reagent during staining operations in existing biological tissue sample section staining devices is time-consuming, labor-intensive, and prone to errors.

[0006] This utility model provides a reagent storage mechanism for a biological tissue sample section staining device, which cooperates with the automatic staining mechanism and reagent addition mechanism of the biological tissue sample section staining device. The reagent addition mechanism has two sets of staining reagent temporary storage components. The automatic staining mechanism has a staining tank cleaning component, a slide discharge cleaning component, and multiple staining tanks for staining individual microscope slides, including a water storage component, a water pumping component, a staining reagent storage component, and a staining reagent pumping component. The water storage component, the water pumping component, the staining reagent storage component, and the staining reagent pumping component are all fixedly mounted on the worktable. The main control console of the biological tissue sample section staining device is controlled and connected to the water pumping component and the staining reagent pumping component. The water storage component is connected to the water pumping component, which in turn works with the staining tank cleaning component and the slide discharge cleaning component. The staining reagent storage component is connected to the staining reagent pumping component, which in turn works with the staining reagent temporary storage component. The staining reagent storage component includes multiple arrayed staining reagent storage containers, which are connected to the staining reagent pumping component. The water pumping component can draw water from the water storage component to the staining tank cleaning component and the slide discharge cleaning component. The staining reagent pumping component can draw staining reagent from the staining reagent storage containers to the staining reagent temporary storage component to replenish the staining reagent in the staining tank.

[0007] In a further improvement to this invention, the water storage component is a water tank with a capacity of not less than 2L.

[0008] This utility model is further improved in that the water pumping assembly includes a diaphragm pump and a two-way valve. The main control console of the biological tissue sample slide staining equipment is connected to the diaphragm pump. One end of the diaphragm pump is connected to the bottom side of the water storage tank, and the other end of the diaphragm pump is connected to the staining tank cleaning assembly and the slide discharge cleaning assembly. The two-way valve is located between the diaphragm pump and the staining tank cleaning assembly and the slide discharge cleaning assembly.

[0009] In a further improvement to this invention, the number of staining reagent storage containers is eight.

[0010] The present invention is further improved in that the capacity of the staining reagent storage container is >1L.

[0011] This invention is further improved in that the staining reagent pumping assembly includes a quantitative pump and a three-way valve. The main control console of the biological tissue sample slide staining equipment is connected to the quantitative pump. One end of the quantitative pump is connected to the bottom side of each of the eight staining reagent storage containers. The other end of the quantitative pump is connected to two sets of staining reagent temporary storage assemblies. The three-way valve is located between the quantitative pump and the staining reagent temporary storage assemblies.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows: This utility model provides a reagent storage mechanism for a biological tissue sample section staining device. By setting up a water storage component, a water pumping component, a staining reagent storage component, and a staining reagent pumping component within the reagent storage mechanism, the water pumping component can draw water from the water storage component to the staining tank cleaning component and the slide discharge cleaning component. The staining reagent pumping component can draw staining reagent from the staining reagent storage container to the staining reagent temporary storage component to replenish the staining tank. This enables automatic addition of supplementary staining reagent to the staining reagent temporary storage component, and automatic replenishment of the staining tank with the staining reagent in the temporary storage component. No manual operation is required, saving time and effort and reducing errors. This significantly improves the production efficiency and product quality of biological tissue sample section microscope slides, enhances the user experience, and solves the problem of time-consuming, labor-intensive, and error-prone manual addition of staining reagent during staining operations in existing biological tissue sample section staining devices. Attached Figure Description

[0013] Figure 1 This is a structural diagram of the reagent storage mechanism of the biological tissue sample section staining device of this utility model.

[0014] In the figure, 3-automatic staining mechanism, 321-staining tank, 35-staining tank cleaning assembly, 36-slide discharge cleaning assembly, 4-reagent adding mechanism, 43-staining reagent temporary storage assembly, 6-reagent storage mechanism, 61-clean water storage assembly, 62-clean water pumping assembly, 63-staining reagent storage assembly, and 64-staining reagent pumping assembly. Detailed Implementation

[0015] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0016] like Figure 1As shown, the present invention provides a reagent storage mechanism 6 for a biological tissue sample section staining device, which cooperates with the automatic staining mechanism 3 and reagent adding mechanism 4 of the biological tissue sample section staining device. The reagent adding mechanism 4 is provided with two sets of staining reagent temporary storage components 43. The automatic staining mechanism is provided with a staining tank cleaning component 35, a slide discharge cleaning component 36, and multiple staining tanks 321 for staining individual microscope slides. It includes a water storage component 61, a water pumping component 62, a staining reagent storage component 63, and a staining reagent pumping component 64. The staining reagent pumping components 64 are all fixedly installed on the worktable. The main control console of the biological tissue sample section staining equipment is connected to the water pumping component 62 and the staining reagent pumping component 64. The water storage component 61 is connected to the water pumping component 62. The water pumping component 62 is coordinated with the staining tank cleaning component 35 and the slide discharge cleaning component 36. The staining reagent storage component 63 is connected to the staining reagent pumping component 64. The staining reagent pumping component 64 is coordinated with the staining reagent temporary storage component 43. The staining reagent storage component 63 includes multiple staining reagent storage containers arranged in an array. The staining reagent storage containers are connected to the staining reagent pumping component 64. In this embodiment, the water pumping component can draw water from the water storage component to the staining tank cleaning component and the slide discharge cleaning component. The staining reagent pumping component can draw staining reagent from the staining reagent storage container to the staining reagent temporary storage component to replenish the staining tank. It can automatically add supplementary staining reagent to the staining reagent temporary storage component, and the staining reagent in the staining reagent temporary storage component can automatically replenish the staining tank without manual operation. This saves time and effort and reduces the risk of errors, greatly improving the production efficiency and product quality of biological tissue sample section microscope slides and enhancing the user experience.

[0017] like Figure 1 As shown, the water storage component is a water tank with a capacity of not less than 2L. The water pumping component includes a diaphragm pump and a two-way valve. The main control console of the biological tissue sample slide staining equipment is connected to the diaphragm pump. One end of the diaphragm pump is connected to the bottom side of the water tank, and the other end is connected to the staining tank cleaning component 35 and the slide discharge cleaning component 336. The two-way valve is located between the diaphragm pump and the staining tank cleaning component 35 and the slide discharge cleaning component 36. In this embodiment, the diaphragm pump can draw water from the water tank according to the control command of the main control console and pump water to the staining tank cleaning component and the slide discharge cleaning component for cleaning the staining tank and microscope slides. The two-way valve acts as a switch for the water pumping channel.

[0018] like Figure 1As shown, there are eight staining reagent storage containers, each with a capacity greater than 1L. The staining reagent pumping assembly 64 includes a metering pump and a three-way valve. The main control console of the biological tissue sample slide staining device is connected to the metering pump. One end of the metering pump is connected to the bottom side of each of the eight staining reagent storage containers, and the other end is connected to two sets of staining reagent temporary storage assemblies 43. The three-way valve is located between the metering pump and the staining reagent temporary storage assembly 43. In this embodiment, the metering pump can quantitatively extract staining reagent from the staining reagent storage containers according to the control instructions of the main control console and quantitatively pump the staining reagent to the staining reagent temporary storage assembly. The staining reagent temporary storage assembly has eight staining reagent temporary storage tanks with a capacity greater than 50ml. Each staining reagent storage container corresponds to one staining reagent temporary storage tank in a set of staining reagent temporary storage assemblies, used for automatically adding and replenishing staining reagent to the staining tank from the staining reagent temporary storage tank. The three-way valve serves as a switch for the staining reagent pumping channel.

[0019] As can be seen from the above, the reagent storage mechanism of the biological tissue sample section staining equipment provided by this utility model, by setting up a water storage component, a water pumping component, a staining reagent storage component, and a staining reagent pumping component that cooperate with each other within the reagent storage mechanism, allows the water pumping component to draw water from the water storage component to the staining tank cleaning component and the slide discharge cleaning component. The staining reagent pumping component draws staining reagent from the staining reagent storage container to the staining reagent temporary storage component to replenish the staining tank. This enables automatic addition of supplementary staining reagent to the staining reagent temporary storage component, and automatic replenishment of the staining tank with staining reagent from the temporary storage component. No manual operation is required, saving time and effort and reducing errors. This significantly improves the production efficiency and product quality of biological tissue sample section microscope slides, enhances the user experience, and solves the problem of time-consuming, labor-intensive, and error-prone manual addition of staining reagent during staining operations in existing biological tissue sample section staining equipment.

[0020] The specific embodiments described above are preferred embodiments of this utility model, and are not intended to limit the specific scope of this utility model. The scope of this utility model includes but is not limited to the specific embodiments described above. All equivalent changes made in accordance with this utility model are within the protection scope of this utility model.

Claims

1. A reagent storage mechanism for a biological tissue sample section staining device, which cooperates with the automatic staining mechanism and reagent adding mechanism of the biological tissue sample section staining device, wherein the reagent adding mechanism is provided with two sets of staining reagent temporary storage components, and the automatic staining mechanism is provided with a staining tank cleaning component, a slide discharge cleaning component, and multiple staining tanks for staining individual microscope slides, characterized in that: The system includes a water storage component, a water pumping component, a staining reagent storage component, and a staining reagent pumping component. All these components are fixedly mounted on a worktable. The main control console of the biological tissue sample slide staining equipment is connected to the water pumping component and the staining reagent pumping component. The water storage component is connected to the water pumping component and works in conjunction with the staining tank cleaning component and the slide discharge cleaning component. The staining reagent storage component is connected to the staining reagent pumping component and works in conjunction with the staining reagent temporary storage component. The staining reagent storage component includes multiple arrayed staining reagent storage containers, which are connected to the staining reagent pumping component. The water pumping component can draw water from the water storage component to the staining tank cleaning component and the slide discharge cleaning component. The staining reagent pumping component can draw staining reagent from the staining reagent storage containers to the staining reagent temporary storage component to replenish the staining tank with staining reagent.

2. The reagent storage mechanism of the biological tissue sample section staining device according to claim 1, characterized in that: The water storage component is a water tank with a capacity of not less than 2L.

3. The reagent storage mechanism of the biological tissue sample section staining device according to claim 2, characterized in that: The water pumping assembly includes a diaphragm pump and a two-way valve. The main control console of the biological tissue sample slide staining equipment is connected to the diaphragm pump. One end of the diaphragm pump is connected to the bottom side of the water storage tank, and the other end of the diaphragm pump is connected to the staining tank cleaning assembly and the slide discharge cleaning assembly. The two-way valve is located between the diaphragm pump and the staining tank cleaning assembly and the slide discharge cleaning assembly.

4. The reagent storage mechanism of the biological tissue sample section staining device according to claim 3, characterized in that: The number of staining reagent storage containers is 8.

5. The reagent storage mechanism of the biological tissue sample section staining device according to claim 4, characterized in that: The capacity of the staining reagent storage container is >1L.

6. The reagent storage mechanism of the biological tissue sample section staining device according to claim 5, characterized in that: The staining reagent pumping assembly includes a metering pump and a three-way valve. The main control console of the biological tissue sample slide staining device is connected to the metering pump. One end of the metering pump is connected to the bottom side of each of the eight staining reagent storage containers. The other end of the metering pump is connected to two sets of staining reagent temporary storage assemblies. The three-way valve is located between the metering pump and the staining reagent temporary storage assemblies.

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