Slide staining jar convenient to add liquid

By using a three-way tube and a metering plate in the slide staining tank, the problems of slide damage and solution waste caused by inaccurate liquid addition were solved, achieving uniform solution coverage and reagent conservation, thus improving staining effect and experimental reliability.

CN223940638UActive Publication Date: 2026-02-24SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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Patent Information

Application Number
CN202520474423.0
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-03-18
Publication Date
2026-02-24
Estimated Expiration
2035-03-18

AI Technical Summary

Technical Problem

Existing slide staining tanks are difficult to control precisely in terms of flow rate and direction when adding liquid, which can easily wash away tissue sections on the slide, leading to experimental failure. Furthermore, spillage can easily occur, resulting in solution waste and increased experimental costs.

Method used

The solution is slowly injected from the bottom of the cylinder using a three-way tube, so that the liquid flow direction is parallel to the plane of the glass slide. The solution flow rate is controlled by a metering plate and regulating valve to avoid direct impact on the tissue section. The glass slide is protected by a soft pad to achieve uniform coverage of the solution and recycling.

Benefits of technology

This reduces the risk of slide damage and uneven staining, decreases solution waste, and improves staining results and experimental reliability.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the field of slide staining jars, in particular to a slide staining jar convenient for adding liquid, which comprises a jar body, a three-way pipe, a funnel, a metering plate and a soft cushion, according to the utility model, the solution is slowly injected from the bottom of the cylinder body through the three-way pipe, so that the liquid flow direction is parallel to the plane of the slide, a tissue slice is prevented from being directly impacted, the damage or falling risk of the slice is reduced, and the solution is slowly injected to flow parallel to the slide, so that the tissue slice can be uniformly covered; the method has the advantages that non-uniform dyeing or overhigh local concentration caused by impact is avoided, the dyeing effect and uniformity are improved, the three-way pipe is used for controlling the solution to be slowly injected into the solution, the liquid adding speed can be adjusted, the liquid flow impact risk is further reduced, excessive use of the solution caused by impact is avoided, and reagent waste is reduced.
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Description

Technical Field

[0001] This utility model relates to the field of glass slide staining tanks, and in particular to a glass slide staining tank that is easy to add liquid to. Background Technology

[0002] The immunohistochemical staining process involves steps such as dewaxing, rehydration, antigen retrieval, blocking, primary and secondary antibody incubation, and staining. In these steps, the slides need to be immersed in different liquids, so a container is needed to hold these liquids and to hold multiple slides for immersion.

[0003] In existing staining jars, liquid is usually poured slowly along the side of the jar to avoid directly impacting the front of the slide. However, manual operation makes it difficult to precisely control the flow rate and direction, which can easily wash away tissue sections on the slide, leading to experimental failure. Furthermore, spillage is common when adding liquid into the staining jar, resulting in solution waste and increased experimental costs.

[0004] Therefore, in existing staining jars, the liquid must be poured slowly along the wall to avoid direct impact on the front of the slide. However, manual operation makes it difficult to precisely control the flow rate and direction, which can easily wash away tissue sections on the slide, leading to experimental failure. Furthermore, spillage is common when adding liquid into the staining jar, resulting in solution waste and increased experimental costs. A new slide staining jar that facilitates liquid addition can be designed, where the solution is slowly injected from the bottom of the jar through a three-way tube, ensuring that the liquid flow direction is parallel to the plane of the slide, avoiding direct impact on the tissue sections and reducing the risk of section damage or detachment. Utility Model Content

[0005] To overcome the problems of existing staining tanks, which are difficult to control precisely in terms of flow rate and direction when operated manually, easily wash away tissue sections on the slides, leading to experimental failure, and easily spill when adding liquid into the staining tank, resulting in solution waste and increased experimental costs.

[0006] The technical solution of this utility model is as follows: a glass slide staining cylinder that is easy to add liquid to includes a cylinder body, a three-way tube, a funnel, a metering plate, and a soft pad. A three-way tube is installed on the outside of the cylinder body. A connecting pipe is provided at the bottom of the outer wall of the cylinder body. The three-way tube is connected and fixed to the cylinder body through the connecting pipe. An adjusting head is installed at the outer end of the three-way tube. A transmission tube is connected and fixed at the upper end of the three-way tube. A funnel is installed at the end of the transmission tube away from the three-way tube.

[0007] Preferably, a measuring plate is provided on one side of the funnel, and two sets of connecting rods are installed between the measuring plate and the funnel.

[0008] Preferably, the inner wall of the cylinder is symmetrically provided with several sets of grooves, and each set of grooves is fitted with a soft pad.

[0009] Preferably, one end of each of the two sets of connecting rods is welded with a connector, and the inside of the metering plate is fitted with a mating hole to accommodate the connector. The connecting rods are then inserted into and fixed to the metering plate via the connector.

[0010] Preferably, a regulating valve is installed at the outer end of the transmission pipe, and the regulating valve is connected to the transmission pipe.

[0011] As a preferred option, the end of the tee pipe furthest from the connecting pipe is equipped with a water outlet pipe, and a connecting plate is welded to one end of the water outlet pipe. The connecting plate has multiple sets of connection holes circumferentially opened inside.

[0012] Preferably, a support frame is installed at the lower end of the cylinder block, and the support frame is fitted and fixed to the cylinder block.

[0013] The beneficial effects of this utility model are:

[0014] Compared to traditional slide staining tanks, this method uses a three-way valve to slowly inject the solution from the bottom of the tank, ensuring the liquid flow is parallel to the plane of the slide. This avoids direct impact on the tissue sections, reducing the risk of damage or detachment. Furthermore, the slow, parallel flow of the solution ensures even coverage of the tissue sections, preventing uneven staining or excessively high local concentrations caused by impact, thus improving staining effectiveness and uniformity. Additionally, the three-way valve allows for controlled, slow injection, adjusting the injection rate and further reducing the risk of liquid flow impact. It also prevents excessive solution usage due to impact, minimizing reagent waste. Attached Figure Description

[0015] Figure 1 The diagram shown is a schematic representation of the overall structure of a slide staining cylinder that facilitates liquid addition according to this utility model.

[0016] Figure 2 The diagram shown is a schematic representation of the structure of a slide staining cylinder funnel that facilitates liquid addition according to this utility model.

[0017] Figure 3 The diagram shown is a schematic representation of the structure of a soft pad for a glass slide staining tank that facilitates liquid addition according to this utility model.

[0018] Figure 4 The diagram shown is a schematic representation of the metering plate structure of a glass slide staining cylinder that facilitates liquid addition according to this utility model.

[0019] Explanation of reference numerals in the attached drawings: 1. Cylinder body; 2. T-connector; 3. Adjusting head; 4. Connecting pipe; 5. Transmission pipe; 6. Funnel; 7. Adjusting valve; 8. Metering plate; 9. Connecting rod; 10. Water outlet pipe; 11. Connecting plate; 12. Soft pad; 13. Support frame. Detailed Implementation

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] Please see Figures 1-4 This utility model provides an embodiment: a slide staining cylinder for easy liquid addition, including a cylinder body 1, a three-way tube 2, a funnel 6, a metering plate 8, and a soft pad 12. The three-way tube 2 is installed on the outside of the cylinder body 1, and a connecting pipe 4 is provided at the bottom of the outer wall of the cylinder body 1. The three-way tube 2 is connected and fixed to the cylinder body 1 through the connecting pipe 4. An adjusting head 3 is installed at the outer end of the three-way tube 2, and a transmission pipe 5 is connected and fixed at the upper end of the three-way tube 2. A funnel 6 is installed at the end of the transmission pipe 5 away from the three-way tube 2.

[0022] Please see Figures 1-3 In this embodiment, a measuring plate 8 is provided on one side of the funnel 6, and two sets of connecting rods 9 are installed between the measuring plate 8 and the funnel 6. By installing the measuring plate 8 on the outside of the funnel 6, the volume of the added solution can be displayed, ensuring the accuracy of the reagent dosage each time staining, avoiding excessive addition of reagent, and using the funnel 6 to provide a stable liquid flow guide, reducing the error of manual pouring and reducing the probability of reagent spillage. Several sets of grooves are symmetrically opened on the inner wall of the cylinder 1, and soft pads 12 are installed inside the grooves. By installing soft pads 12 in the grooves where the glass slides are placed, the direct collision between the glass slides and the cylinder 1 is buffered, avoiding damage to the glass slides due to friction or impact, and providing a certain degree of protection for the glass slides.

[0023] Please see Figures 2-3 In this embodiment, one end of each of the two sets of connecting rods 9 is welded with a connector. The metering plate 8 has a mating hole for accommodating the connector. The connecting rods 9 are fixed to the metering plate 8 by inserting the connector. The metering plate 8 can be disassembled when not in use by using the connector. When the metering plate 8 is not needed, it can be flexibly selected whether to use it according to experimental requirements to meet different usage needs. The outer end of the transmission pipe 5 is equipped with a regulating valve 7, which is connected to the transmission pipe 5. The regulating valve 7 is opened after the specified metered solution is added to the funnel 6 and discharged into the cylinder 1, so as to accurately control the amount and speed of solution addition, avoid over- or under-addition, and ensure the consistency and reliability of the staining effect.

[0024] Please see Figures 2-4 In this embodiment, a water outlet pipe 10 is installed at the end of the three-way pipe 2 away from the connecting pipe 4. A connecting plate 11 is welded to one end of the water outlet pipe 10. Multiple sets of connection holes are opened in the circumferential direction inside the connecting plate 11. By using the connecting plate 11 to assist the water outlet pipe 10 in connecting with other pipes, the solution is sealed and discharged after dyeing, realizing the recycling of the solution. A support frame 13 is installed at the lower end of the cylinder body 1. The support frame 13 is fitted and fixed to the cylinder body 1. By using the support frame 13 to support the cylinder body 1 and place it stably, it is ensured that the dyeing cylinder remains stable during the liquid addition and dyeing process.

[0025] During operation, the three-way pipe 2 is first connected to the connecting pipe 4. The slides to be processed are then placed into the grooves sequentially. The soft pad 12 cushions the direct impact between the slides and the cylinder 1, preventing breakage due to friction or impact and providing some protection. After the slides are placed, the measuring plate 8 is inserted and installed to the connecting rod 9. Simultaneously, other pipes are connected to the outlet pipe 10 using the connecting plate 11. After the main structure is installed, solution is added to the funnel 6. The measuring plate 8 displays the volume of the added solution, ensuring accurate reagent dosage for each staining session and preventing over-addition. After adding the solution, first turn the regulating valve 7, then open the regulating head 3, so that the solution in the funnel 6 flows into the cylinder 1 through the transfer tube 5 and the three-way connector. The solution is slowly injected from the bottom of the cylinder 1, so that the direction of the liquid flow is parallel to the plane of the slide, avoiding direct impact on the tissue section and reducing the risk of damage or detachment of the section. The slow injection of the solution so that it flows parallel to the slide can evenly cover the tissue section and avoid uneven staining or excessive local concentration due to impact. After the slide has been soaked in the solution, adjust the regulating valve 7 to open the outlet pipe 10, control the solution flow rate, and seal and discharge the solution after staining to realize the recycling of the solution.

[0026] Through the above steps, the solution is slowly injected from the bottom of the cylinder 1 using the three-way tube 2, so that the liquid flow direction is parallel to the plane of the glass slide. This avoids direct impact on the tissue sections, reducing the risk of damage or detachment of the sections. Furthermore, the slow injection of the solution, which flows parallel to the glass slide, can evenly cover the tissue sections, avoiding uneven staining or excessively high local concentrations caused by impact, thus improving the staining effect and uniformity. Secondly, using the three-way tube 2 to control the slow injection of the solution allows for adjustment of the liquid addition rate, further reducing the risk of liquid flow impact, while also avoiding excessive use of solution due to impact, thus reducing reagent waste.

Claims

1. A slide staining tank for easy liquid addition, comprising a tank body (1); characterized in that: It also includes a three-way pipe (2), a funnel (6), a metering plate (8), and a soft pad (12). A three-way pipe (2) is installed on the outside of the cylinder body (1). A connecting pipe (4) is provided at the bottom of the outer wall of the cylinder body (1). The three-way pipe (2) is connected and fixed to the cylinder body (1) through the connecting pipe (4). An adjusting head (3) is installed at the outer end of the three-way pipe (2). A transmission pipe (5) is connected and fixed at the upper end of the three-way pipe (2). A funnel (6) is installed at the end of the transmission pipe (5) away from the three-way pipe (2).

2. The slide staining tank for easy liquid addition according to claim 1, characterized in that: A measuring plate (8) is provided on one side of the funnel (6), and two sets of connecting rods (9) are installed between the measuring plate (8) and the funnel (6).

3. The slide staining tank for easy liquid addition according to claim 1, characterized in that: The inner wall of the cylinder (1) is symmetrically provided with several sets of grooves, and each set of grooves is fitted with a soft pad (12).

4. The slide staining tank for easy liquid addition according to claim 2, characterized in that: Two sets of connecting rods (9) have a butt joint welded to one end. The metering plate (8) has a mating hole for accommodating the butt joint. The connecting rods (9) are fixed to the metering plate (8) by inserting the butt joint.

5. A slide staining tank for easy liquid addition according to claim 1, characterized in that: A regulating valve (7) is installed at the outer end of the transmission pipe (5), and the regulating valve (7) is connected to the transmission pipe (5).

6. The slide staining tank for easy liquid addition according to claim 1, characterized in that: The end of the tee pipe (2) away from the connecting pipe (4) is equipped with a water outlet pipe (10), and a connecting plate (11) is welded to one end of the water outlet pipe (10). Multiple sets of connection holes are opened in the circumferential direction inside the connecting plate (11).

7. A slide staining tank for easy liquid addition according to claim 1, characterized in that: A support frame (13) is installed at the lower end of the cylinder body (1), and the support frame (13) is fitted and fixed to the cylinder body (1).