Program cooling device for cryopreserved cells

Abstract

The utility model discloses a programmed cooling device for cryopreserved cells. The programmed cooling device is characterized in that an inner container structure is arranged in a box body; a groove is formed in the inner container; the cryopreservation tube frame is arranged in the groove; a plurality of tube grooves for placing cryopreservation tubes are formed in the cryopreservation tube rack; the flip cover is hinged to one side of the box body. According to the cryopreservation tube, the tube sleeve is arranged in each tube groove, so that an independent temperature protection sleeve device is provided for the cryopreservation tube. When the cryopreservation tube is taken out from a low-temperature environment, the tube sleeve can effectively slow down heat exchange between the cryopreservation tube and an external normal-temperature environment, the phenomenon that cells are repeatedly frozen and thawed due to rapid temperature rise is avoided, and the survival rate and activity of the cells are remarkably improved.

Description

Technical Field

[0001] This utility model relates to the technical field of cryopreservation cells, and in particular to a programmed cooling device for cryopreservation cells. Background Technology

[0002] When cryopreserving cells, the programmed cooling method is commonly used. Specifically, cells are first placed in a programmed cooling chamber, then the chamber is placed in a -80°C freezer overnight. The next day, once the cell solution in the cryovials has also cooled to -80°C, the cryovials are transferred to liquid nitrogen for long-term storage. Programmed cooling chambers are widely used; these freezing containers can programmatically cool cells and are an effective, low-cost cell cryopreservation device. However, current programmed cooling chamber designs generally feature a single-piece lid, and the cryovials lack a temperature-protected sleeve when removed. These shortcomings easily lead to prolonged exposure of cells to room temperature, resulting in the risk of repeated freeze-thaw cycles and frostbite on the experimenter's fingers. Furthermore, commonly used programmed cooling chambers often have poor seals between the cryoprotectant liner and the cryovial rack, leading to frequent leakage of the cryoprotectant and significant risks of contaminating the cryovials and dissolving the markings on them. Utility Model Content

[0003] The technical problem this application aims to solve is how to address the issue of repeated freeze-thaw cycles caused by the lack of a temperature-protected sleeve when retrieving cryopreservation tubes, which leads to cells being easily exposed to room temperature, while also considering how to prevent frostbite on the fingers of laboratory personnel.

[0004] To address the aforementioned technical problems, this application provides a programmed cooling device for cryopreserving cells, comprising:

[0005] The box body has an inner liner structure inside; the inner liner has a groove.

[0006] A cryopreservation tube rack is provided, wherein the cryopreservation tube rack is disposed within the groove; the cryopreservation tube rack is provided with multiple tube slots for placing cryopreservation tubes; each tube slot is provided with a tube sleeve for placing cryopreservation tubes.

[0007] A flip cover, which is hinged to one side of the box body.

[0008] The aforementioned programmed cooling device for cryopreserving cells includes a handle on each side of the housing.

[0009] The above-mentioned programmed cooling device for cryopreserving cells includes a flip cover with through holes corresponding to all the tube slots, and each through hole is provided with a detachably connected tube slot cover.

[0010] The aforementioned programmed cooling device for cryopreserving cells includes a tube sleeve with a handle.

[0011] The program cooling device for cryopreservation cells, wherein the flip cover is provided with an embedded structure towards the box body.

[0012] The program cooling device for cryopreservation cells, wherein the box body is provided with a mounting hole towards the flip cover, and the mounting hole is provided with a clamping part.

[0013] The program cooling device for cryopreservation cells, wherein the top end of the embedded part is a tapered structure.

[0014] The program cooling device for cryopreservation cells, wherein the clamping part comprises a tapered claw structure and a plurality of spherical structures; the end of each branch rod of the tapered claw structure is provided with a spherical structure.

[0015] The program cooling device for cryopreservation cells, wherein the inner container structure is provided with an injection port, the injection port is provided with a recessed hollow structure, and the inner wall of the injection port is provided with an internal thread at the top end.

[0016] The program cooling device for cryopreservation cells, wherein the injection port is provided with a plug, the outer wall of the plug is provided with an external thread, and the external thread is threadedly connected with the internal thread.

[0017] Compared with the prior art, the technical scheme of the present application has the following beneficial effects:

[0018] The utility model discloses a pipe sleeve is arranged in each pipe groove, and a separate temperature protective sleeve device is provided for the cryopreservation tube. When the cryopreservation tube is taken out from the low-temperature environment, the pipe sleeve can effectively slow down the heat exchange between the cryopreservation tube and the normal temperature environment, avoid the repeated freezing and thawing phenomenon of cells due to rapid heating, and significantly improve the survival rate and activity of cells.

[0019] The pipe groove cover is detachable, the flip cover is provided with a through hole corresponding to the pipe groove, and the experimental personnel can operate each cryopreservation tube through the pipe groove cover, so that the whole box cover does not need to be opened, and the time of the cryopreservation tube exposed to the normal temperature is reduced. DETAILED DESCRIPTION

[0020] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings described below only relate to some embodiments of the present application, and are not a limitation on the present application.

[0021] Figure 1 It is a perspective view of the utility model

[0022] Figure 2 It is a schematic view of the flip cover of the utility model

[0023] Figure 3 The sectional view of the embedding structure and the clamping part of the utility model in the installation hole.

[0024] The reference signs are explained as follows:

[0025] Box body: 1

[0026] Inner container structure: 11

[0027] Groove: 12

[0028] Cryopreservation tube rack: 2

[0029] Tube groove: 21

[0030] Tube sleeve: 22

[0031] Flip cover: 3

[0032] Handle: 13

[0033] Through hole: 31

[0034] Tube groove cover: 32

[0035] Embedding structure 33

[0036] Tube sleeve handle: 221

[0037] Installation hole 41

[0038] Clamping part 411

[0039] Conical claw type structure 411a

[0040] Branch rod 411c

[0041] Ball type structure 411b

[0042] Inlet: 111

[0043] Sunken type hollow structure: 112

[0044] Plug portion: 113 DETAILED DESCRIPTION

[0045] To make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be described clearly and completely below with reference to the drawings of the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the described embodiments of the present application, all other embodiments obtained by a person of ordinary skill in the art without any creative effort belong to the scope of protection of the present application.

[0046] Unless otherwise defined, technical terms or scientific terms used herein shall have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terms "first", "second", and similar terms as used herein do not denote any order, quantity, or importance, but are used to identify different components. Also, the terms "a" or "an", as used herein, do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced items.

[0047] As shown in the drawings, a program cooling device for cryopreservation cells comprises: Figures 1 to 3

[0048] A box body 1 is provided with an inner container structure 11 inside. The inner container 11 is provided with a groove 12.

[0049] A cryopreservation tube rack 2 is arranged in the groove 12. The cryopreservation tube rack 2 is provided with a plurality of tube slots 21 for placing cryopreservation tubes. An independent tube sleeve 22 is arranged in each tube slot 21.

[0050] A flip cover 3 is hinged to one side of the box body 1.

[0051] The utility model discloses a plurality of independent tube sleeves 22 are arranged in each tube slot 21, and the tube sleeve 22 is used for placing the cryopreservation tube. The tube sleeve 22 can provide additional thermal insulation protection for the cryopreservation tube, and slow down the heat exchange between the cryopreservation tube and the external environment. When the cryopreservation tube is taken out from the low-temperature environment, the tube sleeve 22 can effectively reduce the temperature rising speed of the cryopreservation tube, avoid the repeated freezing and thawing phenomenon of cells due to rapid heating, and protect the activity and integrity of cells. In addition, the flip cover 3 is hinged to one side of the box body 1, can cover the whole cryopreservation tube rack 2, reduces the contact between the cryopreservation tube rack 2 and the external environment. In addition, the flip cover 3 is provided with through holes corresponding to the tube slots, and each through hole is provided with a detachable tube slot cover. This design allows the experimenter to operate a single cryopreservation tube without opening the flip cover completely, thereby minimizing the time of the cryopreservation tube exposed to normal temperature, and further reducing the risk of repeated freezing and thawing.

[0052] In a preferred embodiment, the two sides of the box body 1 are provided with lifting handles 13 for easy lifting, so that the experimenter can conveniently lift the box body 1.

[0053] In a preferred embodiment, the flip cover 3 is provided with an embedded structure 33 towards the box body 1.

[0054] In a preferred embodiment, the box body 1 is provided with a mounting hole 41 towards the flip cover 3, and the mounting hole 41 is provided with a clamping part 411.

[0055] ​In a preferred embodiment, the top of the embedded structure 33 is provided with a tapered structure. The tapered structure facilitates insertion into the mounting hole 41 on the inner wall of the sleeve and fits tightly with the locking part 411, thereby ensuring that the flip cover 3 can be stably fixed after it is closed on the box body 1.

[0056] In a preferred embodiment, such as Figure 3 As shown, the clamping part 411 includes a conical claw structure 411a and multiple spherical structures 411b; each branch rod 411c of the conical claw structure 411a has a spherical structure 411b at its end. Figure 3 As shown, the conical claw structure 411a is a plastic part with a certain elasticity. Therefore, each branch rod 411c of the conical claw structure 411a has the characteristic of converging towards the center. When the conical structure is inserted, the ball structure 411b will lock the conical structure due to the movement of the ball structure 411b, preventing the tube sleeve handle 221 from loosening or falling off during operation.

[0057] In a preferred embodiment, the flip cover 3 is provided with through holes 31 corresponding to all the tube grooves 21, and each through hole 31 is provided with a detachably connected tube groove cover 32. Specifically, one detachable method is that the tube groove cover 32 can match the hole of the tube groove 21, such as the outer diameter of the tube groove cover 32 and the inner diameter of the tube groove 21 being interference fit, thereby preventing cell leakage from the tube groove 21. Alternatively, another detachable method is that the tube groove cover 32 is provided with a first external thread on the outside, and the tube groove 21 is provided with a first internal thread, with the first external thread threadedly connected to the first internal thread.

[0058] In a preferred embodiment, the sleeve 22 is provided with a sleeve handle 221. The sleeve handle 221 is designed with the operating habits and safety of the experimenters in mind. By providing a convenient gripping area, the experimenters can more easily complete the operation of taking out and putting in the cryopreservation tubes, reducing the risk of frostbite to their fingers.

[0059] In a preferred embodiment, such as Figure 1 As shown, the inner liner structure 11 has an injection port 111, and the injection port 111 has a recessed hollow structure 112 inside. The upper end of the inner wall of the injection port 111 has a second internal thread. The injection port 111 is used to inject cryoprotectant into the inner liner. The injection port 111 has a recessed hollow structure. This design can easily accommodate the plug, and at the same time provide a certain buffer space when injecting cryoprotectant to prevent liquid from overflowing.

[0060] In a preferred embodiment, such as Figure 1 As shown, a plug 113 is provided at the injection port 111, and the outer wall of the plug 113 is provided with a second external thread, which is threadedly connected to the second internal thread. The threaded connection method can ensure a tight fit between the plug and the injection port, effectively preventing leakage of cryoprotectant and the entry of external contaminants.

[0061] The above description is merely exemplary of the application, and is not intended to limit the scope of the application, which is defined by the appended claims.

Claims

1. A programmed cooling device for cryopreserving cells, characterized in that, include: The box body has an inner liner structure inside; the inner liner has a groove. A cryopreservation tube rack is provided within the groove; the cryopreservation tube rack is provided with multiple tube slots for placing cryopreservation tubes; each tube slot is provided with a tube sleeve for placing cryopreservation tubes; A flip cover, which is hinged to one side of the box body.

2. The programmed cooling device for cryopreserving cells according to claim 1, characterized in that, The box has a handle on each side.

3. The programmed cooling device for cryopreserving cells according to claim 1, characterized in that, The flip cover is provided with through holes corresponding to all the tube slots, and each through hole is provided with a detachably connected tube slot cover.

4. The programmed cooling device for cryopreserving cells according to claim 1, characterized in that, The sleeve is equipped with a sleeve handle.

5. The programmed cooling device for cryopreserving cells according to claim 4, characterized in that, The flip cover facing the box body has an embedded structure.

6. The programmed cooling device for cryopreserving cells according to claim 5, characterized in that, The box body has a mounting hole on the side facing the flip cover, and a locking part is provided in the mounting hole.

7. The programmed cooling device for cryopreserving cells according to claim 6, characterized in that, The top of the embedded structure is a conical structure.

8. The programmed cooling device for cryopreserving cells according to claim 7, characterized in that, The clamping part includes a conical claw structure and multiple spherical structures; each branch of the conical claw structure has a spherical structure at its end.

9. The programmed cooling device for cryopreserving cells according to claim 1, characterized in that, The inner liner structure has an injection port, and the injection port has a recessed hollow structure. The upper end of the inner wall of the injection port has an internal thread.

10. A programmed cooling device for cryopreserving cells according to claim 9, characterized in that, The injection port is provided with a plug, and the outer wall of the plug is provided with an external thread, which is threadedly connected to the internal thread.

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