Cell activation dressing with active cell cabin and nutrient communicating channel

Abstract

The utility model discloses a cell activation dressing with an active cell cabin and a nutrient communicating channel, which relates to the technical field of focus tissue repair, and comprises a patch, an active cell cabin and a nutrient communicating channel, the cell treatment nursing carrying table is arranged on the patch and acts on the focus tissue; the bionic structure is arranged in the cell treatment care platform deck and is used for guiding and stimulating neogenesis cell proliferation; the activity adjusting input device is communicated with the cell treatment nursing platform through a conduit and is used for providing nutrients for rapid tissue repair and healing in the basic cell therapy; the cell treatment and care platform deck is used as a carrier of a bionic structure, and the activity regulation input device and the metabolic balance output device are used for providing an effective migration and proliferation space for cells, promoting culture solution drainage supply and metabolism-generated waste liquid drainage and removal, effectively guaranteeing activity regulation and metabolic balance, and improving the treatment effect of the cells. And improvement of the chronic / large-area tissue repair efficiency is facilitated.

Description

TECHNICAL FIELD

[0001] The utility model relates to a lesion tissue repair technical field, specifically relates to a cell activation dressing with active cell cabin and nutrient communication channel. BACKGROUND

[0002] Cell therapy is the most discussed emerging medical treatment in recent years, and the emergence of emerging medical treatment is to solve the limitations of existing treatment.

[0003] Cell therapy is a very broad thing, and common blood transfusion and bone marrow transplantation are a kind of cell therapy. Simply put, the human body receives human cells such as blood cells and bone marrow into the body to achieve the effect of treating diseases and tissue repair.

[0004] The principle of cell therapy is to inject cells (autologous cells) or other cells (allogeneic cells) into the patient's body after laboratory culture or processing technology to achieve the purpose of treating or preventing diseases.

[0005] In cell therapy, autologous cell therapy is safe and has low risk. That is, the patient's cells are taken, then the cells cultured in a qualified GTP laboratory are reinfused or replanted into the body. The whole preparation process is quite rigorous, and it must pass the detection of bacterial count before use, so it is relatively safe to use.

[0006] At present, many regions have opened up cell therapy technologies such as autologous immune cells, autologous adipose stem cells, autologous bone marrow mesenchymal stem cells, autologous fibroblasts, and autologous chondrocytes in clinical practice.

[0007] The materials for cell culture and clinical application of "cell therapy metabolic regulation cell platform / system" need to have the following characteristics:

[0008] High biological compatibility, hydration / hydrophilicity, high anti-infective property, micro-regional tissue culture fluid / metabolic waste fluid drainage property, thermal and structural stability, easy structure design, low pollution residue, cell migration and proliferation.

[0009] At present, it is still difficult to meet all the requirements, especially:

[0010] 1. Biological materials: difficult to design structure, poor mechanical properties, and easy to be infected by biological sources;

[0011] 2. Synthetic materials: poor biological compatibility of multiple functional groups, high chemical residue toxicity, and high pollution.

[0012] The current clinical bottleneck for chronic or large-area tissue repair (bedsore / diabetic wound / war injury) lesion tissue repair and cell therapy:

[0013] 1. Chronic / large area tissue repair: facing the bottleneck of efficient / fast healing.

[0014] 2. The concept of cell therapy can overcome the problem of slow healing of chronic / large area tissue repair.

[0015] 3. Biomedical and clinical tissue healing application technology must face the demand of effective cell migration and proliferation.

[0016] 4. The demand for effective cell migration and proliferation: effective cell migration and proliferation space, culture medium drainage supply, metabolic waste liquid drainage removal, activity regulation, metabolic balance.

[0017] At the same time, the soft biomedical material used in the clinic at present can only form a collapsed structure or a non-connected non-collapsed structure. The collapsed structure or the non-connected non-collapsed structure cannot provide suitable cell migration and proliferation space, and is not conducive to the supply of culture medium drainage and the removal of metabolic waste liquid drainage.

[0018] Therefore, in view of the existing technical problems, the present application provides an active cell therapy care system for chronic or large area tissue repair. Content of the utility model

[0019] The utility model aims at the above-mentioned technical problems, and provides a cell activation dressing with active cell cabin and nutrient communication channel, which aims at solving the above-mentioned problems.

[0020] The utility model provides a cell activation dressing with active cell cabin and nutrient communication channel, which comprises:

[0021] Patch, which is attached to the lesion tissue;

[0022] Cell therapy care platform, which is arranged on the patch and acts on the lesion tissue;

[0023] Bionic structure, which is arranged in the cell therapy care platform and is used for guiding and stimulating the proliferation of new tissue cells;

[0024] Active regulation input device, which is connected to the cell therapy care platform through a catheter, and is used for providing nutrients for basic cell therapy and fast tissue repair and healing;

[0025] Metabolic balance output device, which is connected to the catheter and is used for removing metabolic waste liquid drainage.

[0026] Preferably, the biomimetic structure comprises a three-dimensional cell activation incubation cabin and a metabolic regulation microchannel, the three-dimensional cell activation incubation cabin comprises a three-dimensional cell activation incubation cabin outer wall surface and a three-dimensional cell activation incubation cabin body space, and the metabolic regulation microchannel is connected to the three-dimensional cell activation incubation cabin outer wall surface and the three-dimensional cell activation incubation cabin body space. The patch is attached with an isolation film for isolating the cell treatment and care platform from the outside. The catheter is located between the isolation film and the cell treatment and care platform and is connected to the cell treatment and care platform.

[0027] Preferably, the nutrient is one or more of physiological saline, growth factor liquid, glucose liquid, amino acid liquid, high-oxygen water, high-hydrogen water, and plasma.

[0028] Preferably, a regulator is further arranged on the catheter for regulating the flow of the nutrient in the catheter. The patch is made of silicone material.

[0029] Compared with the prior art, the present application has the following beneficial effects:

[0030] The cell treatment and care platform is used as a carrier of the biomimetic structure, and the active regulation input device and the metabolic balance output device are used to provide a cell effective migration and proliferation space, promote culture liquid drainage supply and metabolic waste liquid drainage removal, effectively guarantee active regulation and metabolic balance, and help improve chronic / large-area tissue repair efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only preferred embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0032] Figure 1 It is a schematic diagram of the cell activation dressing with an active cell cabin and a nutrient communication channel of the present application;

[0033] Figure 2 It is a structural schematic diagram of the cell activation dressing of the present application;

[0034] Figure 3 It is an internal schematic diagram of the cell activation dressing of the present application;

[0035] Figure 4 It is a preparation method schematic diagram of the cell activation dressing of the present application;

[0036] Figure 5 It is an electron microscope diagram of the three-dimensional activated cell incubation cabin of the present application;

[0037] Figure 6The utility model discloses a metabolism regulation microchannel electron microscope graph;

[0038] Figure 7 The utility model discloses a polyvinyl alcohol material electron microscope graph;

[0039] Figure 8 The utility model discloses a HepG2 liver cell electron microscope photograph that grows on polyvinyl butyral (biomimetic type);

[0040] Figure 9 The utility model discloses a HepG2 liver cell electron microscope photograph that grows on polyvinyl butyral (biomimetic type) coated with acellular collagen material;

[0041] Figure 10 It is active cell treatment care platform structure stability analysis schematic diagram for containing biomimetic easy hydration's three dimensional activated cell cultivation cabin;

[0042] Figure 11 It is active cell treatment care platform heat analysis and structure stability / low pollutant residual property schematic diagram for containing biomimetic easy hydration's three dimensional activated cell cultivation cabin;

[0043] Figure 12 It is high power microscope observation diagram for the residual pollution particle and starch residual pollution starch of current similar material domestic and foreign use process technology;

[0044] Figure 13 It is test diagram for the hydrophilic connected space of three dimensional activated cell cultivation cabin after hydration of different aperture size;

[0045] Figure 14 It is the culture condition display diagram of cell treatment care platform in high concentration mould environment.

[0046] In the drawing, 1-patch;2-cell treatment care platform;3-biomimetic structure;31-three dimensional cell activation cultivation cabin;311-three dimensional cell activation cultivation cabin cabin outer wall;312-three dimensional cell activation cultivation cabin cabin space;32-metabolism regulation microchannel;4-active regulation input device;5-metabolic balance output device;6-catheter;7-regulator;8-isolation membrane;9-nutrient;10-lesion;101-neoformation tissue cell;102-autologous cell;103-repair surface;104-autologous cell extraction liquid;105-growth factor liquid;21-culture dish;22-culture solution. DETAILED DESCRIPTION

[0047] In order to more easily understand the structure of the utility model and the function characteristics and advantages that can be achieved, the preferred embodiments of the utility model are described in detail below, and the drawings are as follows:

[0048] Embodiment:

[0049] As Figures 1 to 4 The utility model provides a cell activation dressing with active cell cabin and nutrient communication channel, comprising:

[0050] Patch 1, paste on the lesion 10 tissue, in particular, patch 1 is silica gel material, has adhesion, to be fixed on the lesion 10 tissue,

[0051] Cell therapy care platform 2, be located on patch 1, carry out clean hydration and act on the lesion 10 tissue, namely cover on the lesion 10 tissue,

[0052] Bionic structure 3, be located in cell therapy care platform 2, for guiding stimulation new tissue cell 101 hyperplasia,

[0053] Active regulation inputter 4, communicate on cell therapy care platform 2 through catheter 6, for providing the nutrient 9 of basic cell therapy fast tissue repair healing, and the nutrient 9 of injection can make new cell flush accumulation in the lesion 10, improve repair efficiency,

[0054] Metabolic balance outputter 5, communicate on catheter 6, for metabolic waste liquid drainage removal, and metabolic waste liquid drainage removal can make cell effective healthy growth.The cell activation dressing of the utility model is active cell activation dressing with cell.

[0055] The clinical operation of the cell activation dressing of the utility model:

[0056] The lesion 10 tissue (bed sore / burn and scald / diabetes trauma / war injury) is cleaned and disinfected in advance.

[0057] Utilize cell therapy care platform 2 and clean water combination, and cover on the lesion 10 tissue, provide bionic easy hydration three-dimensional active cell incubation cabin, guide stimulation new tissue cell 101 hyperplasia.

[0058] Utilize active regulation inputter 4 can provide the nutrient 9 of basic cell therapy fast tissue repair healing.

[0059] Utilize metabolic balance outputter 5 to regulate cell growth microenvironment's oxygen, carbon dioxide, nutrient 9, metabolism and culture solution 22 drainage supply, and metabolic waste liquid drainage removal can make cell effective healthy growth.

[0060] Through active regulation inputter 4 injection normal saline can make new cell flush accumulation in the lesion 10, improve repair efficiency.

[0061] Referring to Figure 5, shows the electron microscope graph of the three-dimensional activated cell culture cabin with easy hydration of bionics. In order to effectively repair and heal, it is also necessary to consider the delivery barrier, and effectively drain the external nutrients 9 to the cell growth micro area through the metabolic regulation micro channel 32. The metabolic regulation micro channel 32 can effectively regulate the oxygen, carbon dioxide, nutrients and metabolism of the cell growth micro environment, that is, the culture solution 22 is drained and supplied, the metabolic waste liquid is drained and discharged, and the metabolic balance output device 5 is designed to regulate. The cell treatment and care platform 2 structure can effectively promote the healthy growth of cells. See Figure 6 , shows the electron microscope graph of the metabolic regulation micro channel 32.

[0062] See Figure 7 , shows the electron microscope graph of the polyvinyl alcohol material, which can be seen that the current polyvinyl alcohol process causes insufficient structural support, which is not conducive to the migration and proliferation of HepG2 liver cells.

[0063] See Figure 8 , shows the electron microscope photograph of HepG2 liver cells growing on polyvinyl butyral (bionic type), which gives the proliferation and migration of HepG2 liver cells in the active cell treatment and care platform 2 of the three-dimensional activated cell culture cabin with easy hydration of bionics of the utility model. It can be seen that the space for cell migration and proliferation is conducive to the drainage supply of the culture solution 22 and the drainage discharge of the metabolic waste liquid.

[0064] Specifically, Figure 8 , shows a material of the cell treatment and care platform 2 material of the application. The cell treatment and care platform 2 can also use a material with a hole diameter of 700-1200 microns and a hole wall of not more than 50 microns.

[0065] See Figure 9 , shows the electron microscope photograph of HepG2 liver cells growing on polyvinyl butyral (bionic type) coated with acellular collagen protein material, that is, the proliferation and migration of HepG2 liver cells in the active cell treatment and care platform 2 of the three-dimensional activated cell culture cabin with easy hydration of bionics of the utility model combined with the aid agent.

[0066] See Figure 10 , shows the structural stability analysis of the active cell treatment and care platform 2 of the three-dimensional activated cell culture cabin with easy hydration of bionics of the utility model. According to the double identification thermal analysis results, it is found that the bionic active cell therapy platform in the case only has the weight and energy change of water molecules evaporation below 100 degrees Celsius. No residual or decomposition product is found below 300 degrees Celsius, which shows that the structure of the utility model is clean, safe and stable, has high level of clinical safety, and the clean, safe and stable structure space is conducive to cell growth and improves the benefit of cell therapy.

[0067] SeeFigure 11 , shows the active cell therapy care platform 2 thermal analysis and structural stability / low pollutant residue of the three-dimensional activated cell culture cabin with biomimetic easy hydration, gives the comparison of the current similar material domestic and foreign use process technology and the utility model, can know, the domestic and foreign process technology medical instrument product still has high process pollutant residue (a large number of starch and reactant residue), leads to improve the defective product recall (toxicity) and high clinical infection risk and makes the storage produces mould contamination, makes the management cost greatly promotes.

[0068] Referring to Figure 12 , shows the high-power microscopic observation diagram of the residual pollution particles and starch residue pollution starch caused by the current similar material domestic and foreign use process technology, can know, the residual pollution particles and starch residue pollution starch are more.

[0069] Referring to Figure 13 , shows the test diagram of the three-dimensional activated cell culture cabin of different pore size size after hydration keeps the hydrophilic communication space, the metabolic regulation microchannel 32 after hydration amplifies the siphon and capillary effect (CO3, CO2, nutrient, metabolism), wherein:

[0070] (A) diameter is about 300um:

[0071] (A1) dry type;

[0072] (A2) semi-humid type;

[0073] (A3) wet type;

[0074] (B) diameter is about 500um:

[0075] (B1) dry type;

[0076] (B2) semi-humid type;

[0077] (B3) humid type;

[0078] (C) diameter is about 900um:

[0079] (C1) dry type;

[0080] (C2) semi-humid type;

[0081] (C3) humid type.

[0082] Referring to Figure 14, it is shown that the cell treatment and care platform 2 in the high concentration mold environment can be maintained for more than 30 days without being contaminated, and the material molecular structure is simple and the reaction residue is good in mold resistance. Compared with the domestic and foreign process technology medical instrument products, it is found that serious black mold pollution occurs within 3 days to one week, which causes mold pollution in storage and greatly increases the management cost, mainly because there are still high process pollutants (a large amount of starch and reaction residue) (see Figure 11 thermal analysis and Figure 12 high-power microscopic observation results).

[0083] Referring to Figure 2 and Figure 3 , the bionic structure 3 includes a three-dimensional cell activation cultivation cabin 31 and a metabolic regulation microchannel 32, wherein the metabolic regulation microchannel 32 can effectively and barrier-free regulate the oxygen, carbon dioxide, nutrients and metabolism of the cell growth microenvironment, and can realize the drainage supply of the nutrients 9. The three-dimensional cell activation cultivation cabin 31 includes a three-dimensional cell activation cultivation cabin outer wall surface 311 and a three-dimensional cell activation cultivation cabin body space 312, and the metabolic regulation microchannel 32 is communicated with the three-dimensional cell activation cultivation cabin outer wall surface 311 and the three-dimensional cell activation cultivation cabin body space 312.

[0084] Referring to Figure 1 and Figure 2 , the patch 1 is attached with an isolation film 8 for isolating the cell treatment and care platform 2 from the outside.

[0085] Referring to Figure 1 , the catheter 6 is located between the isolation film 8 and the cell treatment and care platform 2 and is communicated with the cell treatment and care platform 2.

[0086] Referring to Figure 2 , in order to effectively repair and heal, it is necessary to consider the delivery barrier, and the external nutrients 9 are effectively drained to the cell growth micro area through the metabolic regulation microchannel 32 to promote the growth of cells. The nutrients 9 are one or more of physiological saline, growth factor liquid 105, glucose liquid, amino acid liquid, high-oxygen water, high-hydrogen water and blood plasma; it can also be autologous plasma and peptide liquid of high-order cell therapy, and quickly provides the hydrated three-dimensional activated cell cultivation cabin new tissue cells 101.

[0087] Referring to Figure 1 , the application also includes a regulator 7 arranged on the catheter 6 for regulating the flow of nutrients 9 in the catheter 6.

[0088] As shown in Figure 4 , the utility model discloses a preparation method of active cell cabin and nutrient 9 communication channel self-cell type active cell activation dressing, comprising the following steps:

[0089] The cell treatment and nursing platform 2 carrying the three-dimensional cell activation incubation cabin 31 and the metabolic regulation microchannel 32 is placed in the culture dish 21 containing the culture solution 22, the autologous cell extraction solution 104 is extracted to act on the cell treatment and nursing platform 2 and is immersed in the culture solution 22, the growth factor solution 105 is input into the cell treatment and nursing platform 2, the three-dimensional cell activation incubation cabin 31 is used as a space for rapid and large growth of cells, and the two-dimensional cell proliferation environment of the culture dish 21 is changed into a three-dimensional cell proliferation environment. The active cell dressing after culture is covered on the repair surface 103 of the lesion 10, and the proliferated cells adhering to the cells in the three-dimensional cell activation incubation cabin body space 312 are flushed to the repair surface 103 of the lesion 10 by using physiological saline or glucose water, so that the repair efficiency is accelerated.

[0090] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Any person skilled in the art can make many possible changes and modifications to the technical scheme of the present application or modify it into equivalent embodiments with equivalent changes without departing from the technical scheme of the present application. Therefore, any modification, equivalent change and modification made to the above embodiments according to the technical scheme of the present application, which does not depart from the technical scheme of the present application, belongs to the protection scope of the present application.

Claims

1. A cell activation dressing having an active cell compartment and a nutrient communication channel, characterized in that, The patch (1) is attached to the lesion (10) tissue. The cell therapy care platform (2) is arranged on the patch (1) and acts on the lesion (10) tissue. The bionic structure (3) is arranged in the cell therapy care platform (2) and is used for guiding the stimulation of new tissue cell (101) proliferation. The active regulation input device (4) is connected to the cell therapy care platform (2) through the conduit (6) and is used for providing the nutrient (9) for basic cell therapy, rapid tissue repair and healing. The metabolic balance output device (5) is connected to the conduit (6) and is used for draining and removing the metabolic waste liquid. The bionic structure (3) includes a three-dimensional cell activation cultivation cabin (31) and a metabolic regulation microchannel (32), the three-dimensional cell activation cultivation cabin (31) includes a three-dimensional cell activation cultivation cabin outer wall (311) and a three-dimensional cell activation cultivation cabin body space (312), and the metabolic regulation microchannel (32) is connected to the three-dimensional cell activation cultivation cabin outer wall (311) and the three-dimensional cell activation cultivation cabin body space (312).

2. The cell activation dressing with active cell compartment and nutrient communication according to claim 1, wherein, The regulator (7) is arranged on the conduit (6) and is used for regulating the flow of the nutrient (9) in the conduit (6).

3. The cell activation dressing with active cell chamber and nutrient communication according to claim 1, wherein, The patch (1) is made of silica gel material.

4. The cell activation dressing with active cell chamber and nutrient communication according to claim 1, wherein, The patch (1) is attached to the isolation film (8) used for isolating the cell therapy care platform (2) from the outside.

5. The cell activation dressing with active cell pod and nutrient communication according to claim 1, wherein, The conduit (6) is located between the isolation film (8) and the cell therapy care platform (2) and is connected to the cell therapy care platform (2).

6. The cell activating dressing with active cell compartment and nutrient communication according to claim 5, wherein, ​

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