Drosophila olfaction behavior experimental device

Abstract

The utility model discloses a drosophila melanogaster olfaction behavior experimental device, which is characterized in that two smell signal boxes and a testing mechanism are provided, the inside of the testing mechanism is in sliding fit with a base plate through a sliding plate, a counting test tube and a leading-in test tube are externally connected, and the testing mechanism is a transparent device, so that the detection on the smell trend behavior of insects is realized. The operation is convenient, the observation is very easy, and the test result has higher reliability.

Description

Technical Field

[0001] This utility model relates to the field of insect research technology, specifically to an experimental device for fruit fly olfactory behavior. Background Technology

[0002] Insects are diverse in species and form, belonging to the arthropods of the invertebrate family. They are the most numerous animal group on Earth, accounting for over 50% of all biological species, and their presence can be found in almost every corner of the world. Insect behavior is a prerequisite for studying insect physiology and ecology, and is also the foundation for pest control. How to effectively control pests is a hot research topic for scientists. Chemical control, as the main means of pest control, has caused serious "3R" problems (recycling, recycling, and environmental degradation) and environmental problems. With the increasing awareness of environmental protection, scientists have turned their attention to researching more environmentally friendly control measures. Among these, insect behavior modifiers, as a class of specific insecticides, disrupt interspecific information, communication, and feeding behavior of insects, leading to a reduction in population size and thus achieving the purpose of control, and have therefore gradually become a research hotspot.

[0003] Currently, commonly used olfactory instruments include Y-tube olfactometers, four-arm olfactometers, and closed bidirectional selective olfactory instruments. The Y-tube experiment is an important test for determining insect behavioral tendencies; however, there are currently no commercially available products, and laboratory measurements rely on self-assembly, resulting in complex structures and high operational complexity. Four-arm olfactometers are open designs, requiring connection to an exhaust system to maintain odor flow direction, making them relatively expensive. Closed bidirectional selective olfactory instruments cannot recover experimental mosquitoes, leading to difficulties in device cleaning and potentially affecting experimental accuracy. Therefore, there is an urgent need to develop an olfactory device that is simple and reasonable in design, easy to clean, and allows for better control of gas flow direction for studying mosquito odor selection and behavioral responses. Summary of the Invention

[0004] To address the aforementioned problems, this invention proposes an experimental device for fruit fly olfactory behavior, employing the following technical solution.

[0005] An experimental device for fruit fly olfactory behavior includes a testing mechanism and two odor delivery boxes. The testing mechanism includes a first substrate and a second substrate arranged parallel to each other on a base. A slide plate is slidably disposed between the first substrate and the second substrate. The upper and lower parts of the slide plate are respectively provided with cavities and first through holes. The left or right side of the slide plate facing the first substrate is provided with a first capillary extending from the cavity to the outside. The front or rear side of the slide plate is provided with an air outlet, which connects the cavity and the first through hole. The air outlet is externally connected to an air outlet connector.

[0006] The first substrate has a second through hole and a third through hole at its upper and lower parts, respectively, and the second substrate has a fourth through hole at its lower part. The third through hole and the fourth through hole are arranged opposite each other.

[0007] The testing mechanism also includes two counting tubes and one inlet tube. The open end of the inlet tube matches the second through hole of the first substrate. The two open ends of the two counting tubes are respectively inserted into the third through hole of the first substrate and the fourth through hole of the second substrate. The other end of the counting tube is connected to the odor delivery box (1). The odor delivery box is also connected to the flow meter and the air pump.

[0008] Both the first substrate and the second substrate have protruding portions on the side facing the slide plate. The slide plate has recessed grooves on the surface facing the first substrate and the second substrate. The protruding portions cooperate with the grooves, and the slide plate slides between the first substrate and the second substrate.

[0009] The first through hole and the air outlet are connected by a second capillary hole. The second capillary holes are arranged in a row vertically along the height of the slide plate, and the maximum vertical height between the second capillary holes is not greater than the diameter of the first through hole.

[0010] The first through hole, the second through hole, the third through hole, and the fourth through hole have the same diameter.

[0011] The odor delivery box is connected to the counting tube and the flow meter via latex tubing.

[0012] The first substrate, the second substrate, and the slide plate are all transparent acrylic sheets, and the counting tube is a transparent tube.

[0013] Compared with the prior art, the advantages of this utility model are as follows: This device controls the input of fruit flies by moving the slide plate up and down, and the two different odor sources attract insects into the counting test tube, which facilitates the screening of insects of different sizes. It also accurately controls the airflow speed, has a simple structure, is easy to operate, and is easy to clean. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall device;

[0015] Figure 2 A schematic diagram of the structure of a skateboard sliding upwards;

[0016] Figure 3 This is a schematic diagram of a skateboard structure;

[0017] Figure 4 A half-section view of the top view of the skateboard;

[0018] Figure 5 This is a schematic diagram of the first substrate structure;

[0019] Figure 6 This is a schematic diagram of the second substrate structure. Detailed Implementation

[0020] The accompanying drawings are for illustrative purposes only and should not be construed as limiting the present invention. To better illustrate this embodiment, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual product dimensions. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings. The descriptions of positional relationships in the drawings are for illustrative purposes only and should not be construed as limiting the present invention. The present invention will be further described in detail below with reference to specific embodiments.

[0021] like Figure 1-6 As shown, an experimental device for fruit fly olfactory behavior includes a testing mechanism and two odor delivery boxes. The testing mechanism includes a first substrate 4 and a second substrate 7 arranged parallel to each other on a base 6. A sliding plate 5 is slidably disposed between the first substrate 4 and the second substrate 7. Specifically, the left side of the first substrate 4 facing the sliding plate and the right side of the second substrate 7 facing the sliding plate are both provided with protruding portions. The surface of the sliding plate 5 facing the first substrate 4 and the second substrate 7 is provided with recessed grooves, and the protruding portions cooperate with the grooves. The sliding plate 5 slides between the first substrate and the second substrate.

[0022] The upper inner side of the slide plate 5 is provided with a cavity 51, and the lower part is provided with a first through hole 55 penetrating the slide plate. The right side of the slide plate 5 facing the first substrate 4 is provided with a first capillary pore 56. The front side of the slide plate 2 is provided with a sealing plate 57. The front side of the sealing plate 57 is provided with two air outlets 52. Each air outlet 52 is connected to the cavity 51 and the first through hole 55 respectively. The first through hole 55 and the air outlet 52 are provided with a second capillary pore 53 and a hollow block 54 in sequence. When the device is in use, the air outlet is sealed by the air outlet connector. The second capillary pores 53 are arranged in a row vertically along the height of the slide plate 5. The maximum vertical height between the capillary pores is not greater than the diameter of the through hole.

[0023] Furthermore, the upper and lower parts of the first substrate 4 are respectively provided with a second through hole 41 and a third through hole 42 of the same size. The lower part of the second substrate 7 is provided with a fourth through hole 71. The sliding plate 5 slides between the first substrate 4 and the second substrate 7. When sliding, the first through hole 55 can be aligned with the third through hole 42 of the first substrate 4 and the fourth through hole 71 of the second substrate to form a moving channel. The second through hole 41 is aligned with the cavity 51, and the first capillary 56 is directly opposite the second through hole.

[0024] Furthermore, the testing mechanism also includes two counting tubes 8 and one inlet tube 9. The open end of the inlet tube 9 matches the second through hole 41 of the first substrate 1. The air outlets of the two counting tubes 8 are respectively inserted into the third through hole 42 of the first substrate 4 and the fourth through hole 71 of the second substrate 7. The other end of the counting tube 8 is provided with an air inlet interface and is connected to the air outlet interface of the odor delivery box 1 through the latex hose 2.

[0025] Specifically, the top of the odor delivery box 1 has two interfaces. One interface is connected to the counting test tube 6 to supply gas, and the other interface is connected to the gas flow meter 3 through the latex hose 2. The odor delivery box is also connected to an air pump, which is used to supply gas to the odor delivery box. The two odor delivery boxes contain substances with different odors.

[0026] Furthermore, the first substrate 4, the second substrate 7, and the slide plate are all transparent acrylic sheets, and the counting tube is a transparent tube.

[0027] The specific operating steps for using this device are as follows:

[0028] S1. The cavity 51 of the slide plate 5 is aligned with the second through hole 41. At this time, the inlet tube 9 containing insects is connected, and CO2 gas is delivered to the inlet tube 9 through the air outlet 52 to anesthetize the insects.

[0029] S2. Slide the slide plate 5 upwards until the first through hole 55 aligns with the second through hole 41. At this point, pour the test insects from the test tube 9 into the first through hole 55 of the slide plate 5.

[0030] S3. Slide the slide plate 5 downwards, and the first through hole 55 aligns with the third through hole 42 of the first substrate 4 and the fourth through hole 71 of the second substrate to form a moving channel.

[0031] S4. Once the insects awaken, turn on the air pump to deliver the odors from the two odor delivery boxes to the testing mechanism through the latex hose 2;

[0032] S5. Observe and count the number of insects in the two counting tubes, compare the insects' preferences for different odors, and after the experiment is completed, open the apparatus and clean it.

[0033] The second through hole 41 can also accommodate a test tube with electrodes on its inner wall. The test tube is also connected to the odor delivery box 1 through a latex tube. This allows for the application of electric shocks to insects that enter the test tube with electrodes due to the attraction of the odor. After punishing the insects, steps S3 to S5 are repeated to observe whether the insects have any memory of the behavior.

Claims

1. An experimental apparatus for fruit fly olfactory behavior, characterized in that, The test mechanism includes a test mechanism and two odor delivery boxes (1). The test mechanism includes a first substrate (4) and a second substrate (7) arranged in parallel on a base (6). A slide plate (5) is slidably disposed between the first substrate (4) and the second substrate (7). The upper and lower parts of the slide plate (5) are respectively provided with a cavity (51) and a first through hole (55). The left or right side of the slide plate (5) facing the first substrate (4) is provided with a first capillary pore (56) extending from the cavity (51) to the outside. The front or rear side of the slide plate (5) is provided with an air outlet (52). The air outlet (52) connects the cavity (51) and the first through hole (55). The air outlet is connected to an air outlet connector. The first substrate (4) has a second through hole (41) and a third through hole (42) respectively in the upper and lower parts, and the second substrate (7) has a fourth through hole (71) in the lower part. The third through hole (42) and the fourth through hole (71) are arranged opposite each other. The testing mechanism also includes two counting tubes (8) and one inlet tube (9). The open end of the inlet tube (9) matches the second through hole (41) of the first substrate (4). The two open ends of the two counting tubes (8) are respectively inserted into the third through hole (42) of the first substrate (4) and the fourth through hole (71) of the second substrate (7). The other end of the counting tube (8) is connected to the odor delivery box (1). The odor delivery box (1) is also connected to the flow meter (3) and the air pump in sequence.

2. The experimental apparatus for fruit fly olfactory behavior according to claim 1, characterized in that, Both the first substrate (4) and the second substrate (7) have protruding portions on the side facing the slide plate. The slide plate (5) has a recessed groove on the surface facing the first substrate (4) and the second substrate (7). The protruding portions cooperate with the grooves, and the slide plate (5) slides between the first substrate (4) and the second substrate (7).

3. The experimental apparatus for fruit fly olfactory behavior according to claim 1, characterized in that, The first through hole (55) and the air outlet (52) are connected by a second capillary (53). The second capillary (53) is arranged in a row vertically along the height of the slide plate (5). The maximum vertical height between the second capillary (53) is not greater than the diameter of the first through hole (55).

4. The experimental apparatus for fruit fly olfactory behavior according to claim 1, characterized in that, The first through hole (55), the second through hole (41), the third through hole (42), and the fourth through hole (71) have the same diameter.

5. The experimental apparatus for fruit fly olfactory behavior according to claim 1, characterized in that, The odor delivery box (1) is connected to the counting tube (8) and the flow meter (3) via latex tubing (2).

6. The experimental apparatus for fruit fly olfactory behavior according to claim 1, characterized in that, The first substrate (4), the second substrate (7) and the slide plate (5) are all transparent acrylic sheets, and the counting tube (8) is a transparent tube.

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