Device for large-scale propagation of larva parasitic wasps
By designing devices and specific operating procedures for parasitic wasps, the problems of low propagation efficiency and high cost of parasitic wasps in existing technologies have been solved, achieving efficient and low-cost parasitic wasp breeding.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-18
- Publication Date
- 2026-04-03
AI Technical Summary
Existing technologies for controlling lepidopteran noctuid moths have limitations: chemical control leads to excessive pesticide residues and increased pest resistance, while biological control methods, such as parasitic wasps, have low propagation efficiency and high costs, making large-scale application difficult.
Design a device consisting of a host cage and a parasitic wasp rearing cage. Utilize a nylon mesh zipper and hook structure to achieve efficient separation and breeding of the host and parasitic wasps. Combined with a specific operating procedure, improve the parasitism rate and cocoon harvesting rate, and reduce labor time and costs.
It improved the propagation efficiency and cocoon harvesting rate of parasitic wasps, reduced labor costs, and achieved rapid propagation and efficient breeding of parasitic wasps.
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Figure CN224069520U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological control technology of natural enemy insects, particularly to technology related to the large-scale breeding of natural enemy insects, and especially to a device for the large-scale propagation of parasitic wasps larvae. Background Technology
[0002] Noctuidae pests, belonging to the family Noctuidae in the order Lepidoptera, are widely distributed across various countries and regions. Representative species include the beet armyworm (Spodoptera litura Fabricius), the fall armyworm (Spodoptera frugiperda), the beet armyworm (Spodoptera exigua), and the cotton bollworm (Helicoverpa armigera). These pests are characterized by their voracious appetite, omnivorous diet, and high reproductive rate. The larvae feed on plants, causing holes or gaps in leaves, and in severe cases, leading to plant death. In most parts of China, grain crops, garden plants, vegetables, and fruit trees have suffered damage from noctuid moths, resulting in significant economic losses and serious social impacts on agricultural production and the ecological environment.
[0003] Currently, chemical control remains the primary method for controlling noctuid moths. However, the long-term use of chemical pesticides is leading to increasingly prominent problems such as excessive pesticide residues, increased pest resistance, and disruption of the ecological balance. Controlling pests through natural enemies, as an important component of biological control, is a green and safe long-term pest control method. Pest parasitic wasps, as natural enemies of pests, are an important biological control resource. Their large-scale propagation, production, and packaging can enable them to be used as biological control products for pests, offering significant application value for green pest control.
[0004] The spotted lepidopteran wasp (Meteorus pulchricornis) can parasitize lepidopteran larvae and plays an important role in controlling various lepidopteran pests such as the beet armyworm, fall armyworm, cotton bollworm, and others, demonstrating high potential for biological control. The female wasp lays her eggs inside the lepidopteran larvae. After being parasitized, the larvae rapidly reduce their food intake. Once the parasitized larvae mature inside the wasp, they emerge from the lepidopteran larvae, pupate, and eventually die. Utility Model Content
[0005] The technical problem solved by this invention is to provide a device for the large-scale propagation of parasitic wasps larvae.
[0006] The specific technical solution is as follows:
[0007] A device for the large-scale propagation of parasitic wasps larvae comprises two parts: a host cage and a parasitic wasp rearing cage. The host cage has six sides made of nylon mesh of a certain mesh size, and its top nylon mesh has a zipper for introducing and removing the host. The parasitic wasp rearing cage also has six sides made of nylon mesh of a certain mesh size, and its front nylon mesh has a zipper for inserting and removing the host cage. The four corners of the top surface of the parasitic wasp rearing cage each have a hook of a certain length attached to a rope, allowing the host cage to be horizontally suspended inside the parasitic wasp rearing cage by hanging it on the outside of the four corners of the top surface of the parasitic wasp rearing cage. The hooks of a certain length maintain a certain distance between the top nylon mesh of the host cage and the top nylon mesh of the parasitic wasp rearing cage.
[0008] As a preferred option, it consists of two parts: a host holding cage and a parasitic wasp rearing cage.
[0009] Preferably, all six sides of the host container cage are made of 25-30 mesh nylon netting;
[0010] Preferably, the top nylon mesh of the host container cage has a zipper for opening or closing;
[0011] Preferably, the zipper on the top nylon mesh of the parasitic wasp rearing cage is open to allow the host to be introduced and drawn out.
[0012] Preferably, the parasitic wasp rearing cage has hooks on the outside of the four apex corners of the top surface for hanging;
[0013] Preferably, all six sides of the parasitic wasp rearing cage are made of 100-200 mesh nylon mesh;
[0014] Preferably, the parasitic wasp rearing cage has a zipper on the front nylon mesh for opening or closing;
[0015] Preferably, the zipper on the front of the parasitic wasp rearing cage is open so that the host-containing cage can be placed in or removed.
[0016] Preferably, the parasitic wasp rearing cage has rope hooks of a certain length at the four apex corners of its top surface.
[0017] Preferably, the four hooks with ropes of the parasitic wasp rearing cage can be hung on the four corners of the top surface of the host holding cage, and the host holding cage can be horizontally suspended inside the parasitic wasp rearing cage.
[0018] Preferably, the host holding cage is suspended at a distance of 3-5 cm between the top nylon mesh of the host holding cage and the top nylon mesh of the parasitic wasp rearing cage.
[0019] Preferably, the host container cage is a cube with a side length of 30-50cm;
[0020] Preferably, the dimensions of the parasitic wasp rearing cage are: 20-40cm in length, 20-40cm in width, and 4-8cm in height.
[0021] The method for breeding parasitic wasps for large-scale propagation of larvae provided by this utility model is as follows:
[0022] (1) Feeding of larval parasitic wasps and adult wasps: Place the parasitic wasp cocoons that are about to emerge into the parasitic wasp rearing cage. After they emerge, feed them with defatted cotton balls soaked in 10% honey water. Change the honey water cotton balls once a day.
[0023] (2) Parasitism process of parasitic wasps: Place the host of appropriate age into the host holding cage, and then hang the host holding cage in the parasitic wasp rearing cage for 24 hours.
[0024] (3) Host rearing after parasitism: After the parasitism ends, the host cage is removed from the parasitic wasp rearing cage; the host is then introduced into the host rearing tray for continued rearing. Artificial host feed is added every 2 days. On the 7th-8th day after parasitism, the unparasitized hosts are picked out.
[0025] (4) Harvesting of bee cocoons: On the 10th-11th day after parasitism, the bee cocoons are harvested from the host's rearing tray and placed into a container for continued cultivation.
[0026] The above steps (1) to (4) complete one parasitism process. This process is repeated throughout the entire parasitism cycle to achieve the goal of rapid propagation of parasitic wasps. The specific effects are as follows:
[0027] a) It can quickly separate the host and the parasitic wasp. Compared with the traditional parasitism method (introducing and withdrawing adult parasitic wasps multiple times in the parasitism cage), the parasitism operation is more efficient and does not cause any damage to the parasitic wasps.
[0028] b) The breeding method used in conjunction with this device reduces labor time and increases cocoon harvesting rate compared with existing propagation methods, which can significantly reduce the propagation cost of parasitic wasps larvae. Attached Figure Description
[0029] Figure 1 A schematic diagram of the structure of the device for propagating parasitic wasps larvae provided by this utility model;
[0030] Figure 2 A schematic diagram of the structure of the cage for holding the host plant;
[0031] Figure 3 A schematic diagram of the structure of a parasitic wasp rearing cage.
[0032] The following are labeled in the diagram: 1. Host cage; 2. Zipper on the top nylon mesh of the host cage; 3. Hanging openings at the four corners of the top of the host cage; 4. Parasitic wasp rearing cage; 5. Zipper on the front nylon mesh of the parasitic wasp rearing cage; 6. Rope hooks at the four corners of the top of the parasitic wasp rearing cage. Detailed Implementation
[0033] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0034] like Figure 1-3 As shown, this utility model provides a device for propagating parasitic wasps larvae, comprising a host holding cage 1 and a parasitic wasp rearing cage 4. The host holding cage 1 has a zipper 2 on its top nylon mesh and a hanging opening 3 at each of the four top corners. The parasitic wasp rearing cage 4 has a zipper 5 on its front nylon mesh and a rope hook 6 of a certain length at each of the four top corners.
[0035] In one specific implementation, such as Figure 1-3 As shown, the zipper 2 of the top nylon mesh of the host holding cage can be opened to allow the host to be introduced or removed from the host holding cage. The zipper 5 of the front nylon mesh of the parasitic wasp rearing cage can be opened to allow the host holding cage to be placed into or removed from the parasitic wasp rearing cage. The hanging openings 3 at the four corners of the top surface of the host holding cage can be hooked and connected to the rope hooks 6 at the four corners of the top surface of the parasitic wasp rearing cage.
[0036] When in use, the device includes the following:
[0037] ① Rearing of parasitic wasps and adult wasps: Place the cocoons of parasitic wasps that are about to emerge into the parasitic wasp rearing cage. After they emerge, feed them with defatted cotton balls soaked in 10% honey water. Change the honey water cotton balls once a day.
[0038] ② Parasitism process of parasitic wasps in larvae: Place the host of appropriate age into the host holding cage, and then hang the host holding cage in the parasitic wasp rearing cage for 24 hours.
[0039] ③ Host rearing after parasitism: After the parasitism ends, remove the host cage from the parasitic wasp rearing cage; then introduce the host into the host rearing tray for continued rearing, adding artificial host feed every 2 days. On the 7th-8th day after parasitism, remove the unparasitized hosts.
[0040] ④ Harvesting bee cocoons: On the 10th-11th day after parasitism, collect the bee cocoons from the host's rearing tray and transfer them to a container for continued cultivation.
[0041] The above steps ① to ④ complete one parasitism process, and this cycle repeats throughout the entire parasitism process to achieve the goal of rapid proliferation of parasitic wasps.
[0042] Example 1: A parasitic rice moth on a spotted chrysalis wasp
[0043] (1) Test insect source: The parasitic wasp was *Sclerotium scabra*, and the host was the 6th instar rice moth larva.
[0044] (2) Host cage: a cubic insect cage of 30*30*5cm with 30 meshes on 6 sides; Parasitic wasp cage: a rectangular insect cage of 40*40*40cm with 100 meshes on 6 sides.
[0045] (3) Operation process:
[0046] ① Rearing of larval parasitic wasps and adult wasps: Place 200 parasitic wasp cocoons that are about to emerge into a parasitic wasp rearing cage. After they emerge, feed them with defatted cotton balls soaked in 10% honey water. Change the honey water cotton balls once a day.
[0047] ② Parasitism process of parasitic wasps: When the parasitic wasps are 4 days old, 2,000 6th instar rice moth larvae of suitable age are introduced into the host cage, and then the host cage is hung in the parasitic wasp rearing cage for 24 hours.
[0048] ③ Host rearing after parasitism: After the parasitism ends, remove the host cage from the parasitic wasp rearing cage; then introduce the rice moth into the host rearing tray for continued rearing, adding artificial feed for the rice moth host every 2 days. On the 7th-8th day after parasitism, pick out the unparasitized rice moth hosts.
[0049] ④ Collection of bee cocoons: On the 10th-11th day after parasitism, collect the bee cocoons from the host's rearing tray into a container, and count the number of rice moth larvae parasitized and the number of cocoons collected by the parasitic wasps.
[0050] Parasite quantity: The number of rice moth larvae that are parasitized.
[0051] Parasitism rate = number of parasites / total number of hosts
[0052] Cocoon count: The number of parasitic wasp cocoons formed after the rice moth larvae are parasitized.
[0053] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants
[0054] The control treatment was repeated 5 times, and finally the data were processed and analyzed.
[0055] Control 1: A parasitic rice moth on a spotted chrysanthemum wasp
[0056] (1) The source of the test insects: the parasitic wasp was *Sclerotium spp.* and the host was *Eriocheir sinensis*.
[0057] (2) Parasitic container: Parasitic cage 1 is a cubic insect rearing cage of 40*40*40cm, with 100 meshes of nylon mesh on 6 sides.
[0058] (3) Operation process:
[0059] ① Rearing of larval parasitic wasps and adult wasps: Place 200 parasitic wasp cocoons that are about to emerge into parasitic cage 1. After they emerge, feed them with defatted cotton balls soaked in 10% honey water. Change the honey water cotton balls once a day.
[0060] ② Parasitism process of parasitic wasps: When the parasitic wasps are 4 days old, 2000 6th instar rice moth larvae of suitable age are introduced into parasitism cage 1 and parasitized for 24 hours.
[0061] ③ Host rearing after parasitism: After the parasitism ends, remove the rice moth larvae one by one from the parasitism cage 1; place the rice moths in the host rearing tray for continued rearing, adding artificial feed for the rice moth hosts every 2 days, and pick out the unparasitized rice moth hosts 7-8 days after parasitism.
[0062] ④ Collection of bee cocoons: On the 10th-11th day after parasitism, collect the bee cocoons from the host's rearing tray into a container, and count the number of rice moth larvae parasitized and the number of cocoons collected by the parasitic wasps.
[0063] Parasite quantity: The number of rice moth larvae that are parasitized.
[0064] Parasitism rate = number of parasites / total number of hosts
[0065] Cocoon count: The number of parasitic wasp cocoons formed after the rice moth larvae are parasitized.
[0066] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants
[0067] The control treatment was repeated 5 times, and finally the data were processed and analyzed.
[0068] Comparing Example 1 with Control Example 1, it can be seen that: (1) the parasitism rate of *Hypericum spp.* in Example 1 is higher; (2) the cocoon harvesting rate of parasitoid wasps in Example 1 is higher; (3) the labor time required for the parasitoid wasp propagation method in Example 1 is about 67% of that in Control Example 1, which reduces labor costs.
[0069] Table 1 Comparison of propagation results of *Sparganum sp.* using *Paecilomyces cerevisiae* as a host.
[0070]
[0071]
[0072] Example 2: A parasitic armyworm on a lenticel wasp.
[0073] (1) The source of the test insects: the parasitic wasp was *Sclerotium spp.* and the host was the larvae of the armyworm at the end of the second instar.
[0074] (2) Host cage: a cubic insect cage of 25*25*5cm with 25 meshes on 6 sides; Parasitic wasp cage: a rectangular insect cage of 35*35*35cm with 120 meshes on 6 sides.
[0075] (3) Operation process:
[0076] ① Rearing of larval parasitic wasps and adult wasps: Place 150 parasitic wasp cocoons that are about to emerge into a parasitic wasp rearing cage. After they emerge, feed them with defatted cotton balls soaked in 10% honey water. Change the honey water cotton balls once a day.
[0077] ② Parasitism process of parasitic wasps: When the parasitic wasps are 4 days old, 1500 2nd instar armyworm larvae of appropriate age are introduced into the host cage, and then the host cage is hung in the parasitic wasp rearing cage for 24 hours.
[0078] ③ Host rearing after parasitism: After the parasitism ends, remove the host cage from the parasitic wasp rearing cage; then introduce the armyworms into the host rearing tray for continued rearing, adding armyworm host artificial feed every 2 days. On the 7th-8th day after parasitism, remove the armyworm hosts that have not been parasitized.
[0079] ④ Collection of bee cocoons: On the 10th-11th day after parasitism, collect the bee cocoons from the host's rearing tray into a container, and count the number of armyworm larvae parasitized and the number of cocoons collected from the parasitic bees.
[0080] Parasite quantity: The number of armyworm larvae that are parasitized.
[0081] Parasitism rate = number of parasites / total number of hosts
[0082] Cocoon quantity: The number of parasitic wasp cocoons formed after armyworm larvae are parasitized.
[0083] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants
[0084] The control treatment was repeated 5 times, and finally the data were processed and analyzed.
[0085] Control 2: *Ichthyophthirius multifiliis* parasitized by the worm *Armella*
[0086] (1) The source of the test insects: the parasitic wasp was *Sclerotium spp.* and the host was armyworm.
[0087] (2) Parasitic container: Parasitic cage 2 is a cubic insect rearing cage of 35*35*35cm, with 120 meshes of nylon mesh on 6 sides.
[0088] (3) Operation process:
[0089] ① Rearing of larval parasitic wasps and adult wasps: Place 150 parasitic wasp cocoons that are about to emerge into parasitic cage 2. After they emerge, feed them with defatted cotton balls soaked in 10% honey water. Change the honey water cotton balls once a day.
[0090] ② Parasitism process of parasitic wasps: When the parasitic wasps are 4 days old, 1500 armyworm larvae of appropriate age at the end of the second instar are introduced into parasitism cage 2 and parasitized for 24 hours.
[0091] ③ Host rearing after parasitism: After the parasitism ends, remove the armyworm larvae one by one from the parasitism cage 2; place the armyworms in the host rearing tray for continued rearing, add armyworm host artificial feed every 2 days, and pick out the unparasitized armyworm hosts on the 7th-8th day after parasitism.
[0092] ④ Collection of bee cocoons: On the 10th-11th day after parasitism, collect the bee cocoons from the host's rearing tray into a container, and count the number of armyworm larvae parasitized and the number of cocoons collected from the parasitic bees.
[0093] Parasite quantity: The number of armyworm larvae that are parasitized.
[0094] Parasitism rate = number of parasites / total number of hosts
[0095] Cocoon quantity: The number of parasitic wasp cocoons formed after armyworm larvae are parasitized.
[0096] Cocoon harvest rate = Number of cocoons harvested / Total number of host plants
[0097] The control treatment was repeated 5 times, and finally the data were processed and analyzed.
[0098] Comparing Example 2 with Control Example 2, it can be seen that: (1) the parasitism rate of the propagated *Hymenochloa spp.* in Example 2 is also higher; (2) the cocoon harvesting rate of the parasitic wasps of *Hymenochloa spp.* in Example 2 is also higher; (3) the labor time required for the parasitic wasp propagation method in Example 2 is about 61% of that in Control Example 2, which reduces labor costs.
[0099] Table 2 Comparison of propagation results of *Brachysmus stigmatis* using armyworms as hosts.
[0100]
[0101]
[0102] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.
Claims
1. A device for the large-scale propagation of parasitic wasps larvae, characterized in that, It consists of two parts: a host cage and a parasitic wasp rearing cage. The host cage has hooks at the four corners of its top surface. The parasitic wasp rearing cage has 100-200 mesh nylon mesh on all six sides. The front nylon mesh of the parasitic wasp rearing cage has a zipper for opening and closing. When the zipper on the front nylon mesh of the parasitic wasp rearing cage is open, the host cage can be placed in or removed. The host cage has 25-30 mesh nylon mesh on all six sides. The top nylon mesh of the host cage has a zipper for opening and closing. When the zipper on the top nylon mesh is open, the host can be introduced and withdrawn. The parasitic wasp rearing cage has four hooks with ropes of a certain length at the four corners of its top surface. These four hooks can be hung on the four corners of the host cage, allowing the host cage to be suspended horizontally inside the parasitic wasp rearing cage. The distance between the top nylon mesh of the host cage and the top nylon mesh of the parasitic wasp rearing cage is 3-5 cm.
2. The device for large-scale propagation of parasitic wasps larvae according to claim 1, characterized in that, The host container cage is a cube with a side length of 30-50cm.
3. The device for large-scale propagation of parasitic wasps larvae according to claim 1, characterized in that, The dimensions of the parasitic wasp rearing cage are: 20-40cm in length, 20-40cm in width, and 4-8cm in height.