Device for improving protein expression effect

Abstract

The utility model discloses a device for improving protein expression effect, which comprises a tank body, an air supply component is arranged outside the tank body, the air supply component comprises an extension plate II fixedly connected with the bottom of the outer wall of the circumference of the tank body, and a hydraulic cylinder vertically arranged is fixedly arranged on the outer wall of the top of the extension plate II. A connecting arm is fixedly mounted at the output end of the hydraulic cylinder through a bolt, a mounting sleeve is fixedly connected to the end of the connecting arm, an air supply pipe is rotatably connected to the interior of the mounting sleeve, one end of the bottom of the air supply pipe extends into the tank body, and a first extension plate is fixedly connected to the circumferential outer wall of the tank body; and a sterile air compressor is fixedly mounted on the outer wall of the top of the extension plate I. The air supply assembly is arranged, so that the air collecting pipe can be driven to enter the product when microorganisms need to supply oxygen, and is contracted to the outside of the product in a non-working period, the risk of backward flowing of fermentation liquor is isolated from a physical structure, and the cleaning and maintenance difficulty and the bacterial contamination risk are remarkably reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to fermentation equipment technical field, concretely for a device for improving protein expression effect. BACKGROUND

[0002] Protein expression refers to the process that through genetic engineering technology, foreign genes are introduced into host cells, so that the host cells can synthesize and express target proteins. Protein expression technology has extremely important application value in many fields such as biopharmaceuticals, biological research, disease diagnosis, etc.

[0003] Through the search, the utility model with patent announcement number CN221152796U discloses a fermentation tank for fermentation in the dairy fermentation technical field, which comprises a tank body, a discharge pipe is arranged at the bottom plate of the tank body, a feeding mechanism is arranged at the upper end of the side wall of the tank body, a temperature adjusting mechanism is arranged outside the side wall of the tank body, a liquid distribution box is fixedly arranged on the upper end face of the top plate of the tank body, a motor is fixedly arranged on the liquid distribution box, and a vertical transmission pipe is fixedly arranged on the output shaft of the motor.

[0004] The above device continuously introduces sterile air into the fermentation tank through the ventilation system to provide sufficient oxygen for the growth of microorganisms or cells, promote the synthesis and expression of proteins, but the nozzle is always inside the tank body, and liquid will enter the pipeline through the nozzle. If a bacterial film is formed inside the pipeline, it is difficult to clean up, and there is room for improvement. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a device for improving protein expression effect to solve the problems in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a device for improving protein expression effect, comprising a tank body, a gas supply assembly is installed outside the tank body, the gas supply assembly comprises an extension plate two fixedly connected at the bottom of the tank body circumferential outer wall, a hydraulic cylinder vertically arranged is fixedly installed on the top outer wall of the extension plate two, a connecting arm is fixedly installed on the output end of the hydraulic cylinder through bolts, an installation sleeve is fixedly connected at the end of the connecting arm, a gas supply pipe is rotatably connected in the installation sleeve, and one end of the gas supply pipe bottom extends into the tank body.

[0007] The gas collecting pipe can be driven into the inside of the product when the microorganism needs oxygen supply, and is retracted to the outside of the product during the non-working period, thereby physically isolating the risk of backflow of the fermentation liquid, significantly reducing the difficulty of cleaning and maintenance and the risk of contamination, starting the sterile air compressor to start air supply, then starting the hydraulic cylinder at the top of the extension plate two, the movable end of the hydraulic cylinder drives the connecting arm to move downward, the connecting arm drives the air supply pipe to move synchronously through the mounting sleeve, and the gas collecting pipe mounted at the bottom of the air supply pipe enters the fermentation liquid. Due to the continuous injection of sterile air, the fermentation liquid cannot backflow into the gas collecting pipe and the air supply pipe, and when oxygen supply is not needed, the gas collecting pipe can be driven to separate from the fermentation liquid by the hydraulic cylinder, thereby avoiding the formation of biofilm in the pipeline from the root.

[0008] As a further preferred aspect of the present technical solution, the extension plate one is fixedly connected to the circumferential outer wall of the tank body, the sterile air compressor is fixedly installed on the top outer wall of the extension plate one, and the same connecting pipe is installed between the air outlet of the sterile air compressor and the mounting sleeve.

[0009] As a further preferred aspect of the present technical solution, the tank cover is installed on the top outer wall of the tank body, the exhaust port is installed on the top of the tank cover, the first one-way valve is installed inside the exhaust port, and the air supply pipe is slidingly inserted into the tank cover.

[0010] As a further preferred aspect of the present technical solution, the exhaust assembly is installed at one end of the bottom of the air supply pipe, the exhaust assembly includes the gas collecting pipe fixedly installed at the bottom of the air supply pipe, a plurality of horizontally arranged push nozzles one are arranged on one side of the circumferential outer wall of the gas collecting pipe, a plurality of horizontally arranged push nozzles two are arranged on the other side of the circumferential outer wall of the gas collecting pipe, and the push nozzles one and the push nozzles two are oppositely arranged.

[0011] After the sterile air enters the gas collecting pipe, it will be sprayed outward through the push nozzles one, the push nozzles two and the air outlet nozzles. Since the directions of the push nozzles one and the push nozzles two are designed to be opposite, when the sterile air is sprayed from the two nozzles, according to the principle of mechanics, the directions of the reaction forces generated by the air flows sprayed from the two nozzles will also be opposite. The reaction forces in opposite directions act on the gas collecting pipe, thereby forming a moment, which drives the gas collecting pipe to rotate, so that the distribution of air is wider, which is conducive to increasing the dissolved oxygen content and improving the protein expression effect. The rotation of the gas collecting pipe also plays a certain stirring role, further improving the protein expression effect.

[0012] As a further preferred aspect of the present technical solution, a plurality of air outlet nozzles are arranged at the bottom of the circumferential outer wall of the gas collecting pipe, and a mesh plate is arranged inside the outlet of the air outlet nozzles.

[0013] As a further preferred aspect of the present technical solution, a second one-way valve is installed inside the air supply pipe.

[0014] As a further preferred of the technical scheme, the gas supply pipe and the gas collecting pipe are made of polypropylene material.

[0015] The device for improving protein expression effect has the following beneficial effects:

[0016] (1) The gas supply assembly is arranged, the gas collecting pipe is driven to enter the inside of the product when the microorganism needs oxygen supply, and the gas collecting pipe is retracted to the outside of the product during the non-working period, thereby avoiding the risk of backflow of the fermentation liquid from the physical structure, significantly reducing the cleaning and maintenance difficulty and the risk of bacterial contamination, starting the sterile air compressor to supply air first, then starting the hydraulic cylinder at the top of the extension plate II, the movable end of the hydraulic cylinder drives the connecting arm to move downward, the connecting arm drives the gas supply pipe to move synchronously through the mounting sleeve, and the gas collecting pipe arranged at the bottom of the gas supply pipe will enter the fermentation liquid, due to the continuous injection of sterile air, the fermentation liquid will not flow back into the gas collecting pipe and the gas supply pipe, and when oxygen supply is not needed, the gas collecting pipe can be driven by the hydraulic cylinder to separate from the fermentation liquid, thereby avoiding the formation of biofilm in the pipeline from the root.

[0017] (2) The gas exhaust assembly is arranged, after the sterile air enters the inside of the gas collecting pipe, the sterile air is sprayed outward through the push nozzle I, the push nozzle II and the air outlet nozzle, since the directions of the push nozzle I and the push nozzle II are designed to be opposite, when the sterile air is sprayed from the two nozzles, according to the mechanical principle, the directions of the reaction forces generated by the air flows sprayed from the two nozzles are also opposite, the reaction forces in the opposite directions act on the gas collecting pipe, thereby forming a moment, so that the gas collecting pipe is driven to rotate, the distribution of air is wider, the dissolved oxygen content is increased, the protein expression effect is improved, and the rotation of the gas collecting pipe also plays a certain stirring role, thereby further improving the protein expression effect. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a whole first visual angle structure schematic diagram of the utility model;

[0019] Figure 2 It is a whole second visual angle structure schematic diagram of the utility model;

[0020] Figure 3 It is an exhaust assembly enlarged structure schematic diagram of the utility model;

[0021] Figure 4 It is a Figure 2 It is a whole first visual angle structure schematic diagram of the utility model;

[0022] In the figure: 1, tank body; 2, tank cover; 3, extension plate one; 4, sterile air compressor; 5, air supply assembly; 6, exhaust assembly; 501, extension plate two; 502, hydraulic cylinder; 503, connecting arm; 504, mounting sleeve; 505, air supply pipe; 506, connecting pipe; 601, gas collecting pipe; 602, push nozzle one; 603, push nozzle two; 604, air outlet nozzle. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model.

[0024] The utility model provides technical schemes: as Figure 1 and Figure 4 As shown in the figure, in the embodiment, a device for improving protein expression effect, including tank body 1, tank body 1 outside is equipped with air supply assembly 5, air supply assembly 5 includes the extension plate two 501 of tank body 1 circumferential outer wall bottom fixed connection, the hydraulic cylinder 502 of vertical setting is fixedly installed on the top outer wall of extension plate two 501, the connecting arm 503 of hydraulic cylinder 502 output end is fixedly installed through bolt, the mounting sleeve 504 of connecting arm 503 end is fixedly connected, the air supply pipe 505 of mounting sleeve 504 inside rotation is connected, and the air supply pipe 505 bottom one end extends into the inside of tank body 1.

[0025] Tank body 1 circumferential outer wall is fixedly connected with extension plate one 3, and sterile air compressor 4 (preferably model Atlas Copco GA series GA 30+-90+) is fixedly installed on the top outer wall of extension plate one 3, and the same connecting pipe 506 is installed between the air outlet of sterile air compressor 4 and mounting sleeve 504.

[0026] First, start sterile air compressor 4 to start air supply, then start hydraulic cylinder 502 on the top of extension plate two 501, and the movable end of hydraulic cylinder 502 drives connecting arm 503 to move downward, and connecting arm 503 drives air supply pipe 505 to move synchronously through mounting sleeve 504, and gas collecting pipe 601 installed at the bottom of air supply pipe 505 will enter into fermentation broth, and due to the continuous injection of sterile air, fermentation broth will not flow back into gas collecting pipe 601 and air supply pipe 505, and when oxygen supply is not needed, gas collecting pipe 601 can be driven to separate from fermentation broth by hydraulic cylinder 502, to avoid the formation of biofilm in the pipeline from the root.

[0027] As Figure 1 and Figure 2 As shown in the figure, tank cover 2 is installed on the top outer wall of tank body 1, the top of tank cover 2 is installed with an air outlet, a first check valve is installed in the air outlet, air supply pipe 505 is slidingly inserted into tank cover 2, and after the air in tank body 1 is increased, the excess air will pass through the first check valve and be discharged outward through the air outlet, but the air outside will not enter the inside of tank body 1.

[0028] As Figure 2 And Figure 3 As shown in the bottom of the gas supply pipe 505 one end is provided with exhaust assembly 6, exhaust assembly 6 includes the bottom of the gas supply pipe 505 fixedly installed gas collector 601, the circumference of the outer wall of the gas collector 601 one side is provided with a plurality of horizontally arranged push nozzle one 602, the circumference of the outer wall of the gas collector 601 the other side is provided with a plurality of horizontally arranged push nozzle two 603, push nozzle one 602 and push nozzle two 603 are oppositely arranged.

[0029] The circumference of the outer wall of the gas collector 601 bottom is provided with a plurality of equidistantly distributed gas outlet nozzles 604, and the outlet of the gas outlet nozzle 604 is internally provided with a screen plate. After the airflow passes through the screen plate, a large number of small bubbles are formed, so that the contact area of the fermentation broth and the bubbles is larger, and the dissolved oxygen content can be increased.

[0030] After the sterile air enters the inside of the gas collector 601, it will be sprayed outward through the push nozzle one 602, the push nozzle two 603 and the gas outlet nozzle 604. Since the push nozzle one 602 and the push nozzle two 603 are designed to be in opposite directions, when the sterile air is sprayed from the two nozzles, according to the mechanical principle, the reaction forces generated by the airflow sprayed from the two nozzles will also be in opposite directions. The reaction forces in opposite directions acting on the gas collector 601 will form a moment, thereby driving the gas collector 601 to rotate, so that the distribution of air is wider, which is beneficial to increase the dissolved oxygen content and improve the protein expression effect. The rotation of the gas collector 601 also plays a certain stirring role, further improving the protein expression effect.

[0031] As shown in the bottom of the gas supply pipe 505 one end is provided with exhaust assembly 6, exhaust assembly 6 includes the bottom of the gas supply pipe 505 fixedly installed gas collector 601, the circumference of the outer wall of the gas collector 601 one side is provided with a plurality of horizontally arranged push nozzle one 602, the circumference of the outer wall of the gas collector 601 the other side is provided with a plurality of horizontally arranged push nozzle two 603, push nozzle one 602 and push nozzle two 603 are oppositely arranged. Figure 3 As shown in the bottom of the gas supply pipe 505 one end is provided with exhaust assembly 6, exhaust assembly 6 includes the bottom of the gas supply pipe 505 fixedly installed gas collector 601, the circumference of the outer wall of the gas collector 601 one side is provided with a plurality of horizontally arranged push nozzle one 602, the circumference of the outer wall of the gas collector 601 the other side is provided with a plurality of horizontally arranged push nozzle two 603, push nozzle one 602 and push nozzle two 603 are oppositely arranged.

[0032] As shown in the bottom of the gas supply pipe 505 one end is provided with exhaust assembly 6, exhaust assembly 6 includes the bottom of the gas supply pipe 505 fixedly installed gas collector 601, the circumference of the outer wall of the gas collector 601 one side is provided with a plurality of horizontally arranged push nozzle one 602, the circumference of the outer wall of the gas collector 601 the other side is provided with a plurality of horizontally arranged push nozzle two 603, push nozzle one 602 and push nozzle two 603 are oppositely arranged. Figure 2 As shown in the bottom of the gas supply pipe 505 one end is provided with exhaust assembly 6, exhaust assembly 6 includes the bottom of the gas supply pipe 505 fixedly installed gas collector 601, the circumference of the outer wall of the gas collector 601 one side is provided with a plurality of horizontally arranged push nozzle one 602, the circumference of the outer wall of the gas collector 601 the other side is provided with a plurality of horizontally arranged push nozzle two 603, push nozzle one 602 and push nozzle two 603 are oppositely arranged. Figure 3 As shown in the bottom of the gas supply pipe 505 one end is provided with exhaust assembly 6, exhaust assembly 6 includes the bottom of the gas supply pipe 505 fixedly installed gas collector 601, the circumference of the outer wall of the gas collector 601 one side is provided with a plurality of horizontally arranged push nozzle one 602, the circumference of the outer wall of the gas collector 601 the other side is provided with a plurality of horizontally arranged push nozzle two 603, push nozzle one 602 and push nozzle two 603 are oppositely arranged.

[0033] The utility model provides a kind of device for improving protein expression effect, specific working principle as follows:

[0034] When the device needs oxygen supply, first start the sterile air compressor 4 to start air supply, then start the hydraulic cylinder 502 at the top of the extension plate two 501, the movable end of the hydraulic cylinder 502 drives the connecting arm 503 to move downward, the connecting arm 503 drives the air supply pipe 505 to move synchronously through the mounting sleeve 504, the gas collecting pipe 601 mounted at the bottom of the air supply pipe 505 will enter the fermentation liquid, due to the continuous injection of sterile air, the fermentation liquid will not flow into the gas collecting pipe 601 and the air supply pipe 505, when no oxygen supply is needed, the gas collecting pipe 601 can be driven by the hydraulic cylinder 502 to separate from the fermentation liquid, which can avoid the formation of biofilm in the pipeline from the source, after the sterile air enters the inside of the gas collecting pipe 601, it will be sprayed outward through the push nozzle one 602, the push nozzle two 603 and the air outlet nozzle 604, since the directions of the push nozzle one 602 and the push nozzle two 603 are designed to be opposite, when the sterile air is sprayed from the two nozzles, according to the mechanical principle, the directions of the reaction forces generated by the air flows sprayed from the two nozzles will also be opposite, the reaction forces in opposite directions act on the gas collecting pipe 601, a moment is formed, so as to drive the gas collecting pipe 601 to rotate, so that the distribution of air is wider, which is beneficial to increase the dissolved oxygen content and improve the protein expression effect, the rotation of the gas collecting pipe 601 can also play a certain stirring role, which further improves the protein expression effect.

[0035] Although the embodiments of the present application have been shown and described, it should be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A device for improving the expression effect of a protein, comprising a tank body (1), characterized in that: The tank body (1) is externally provided with a gas supply assembly (5), the gas supply assembly (5) comprises an extension plate two (501) fixedly connected to the bottom of the outer wall of the circumference of the tank body (1), the top outer wall of the extension plate two (501) is fixedly installed with a vertically arranged hydraulic cylinder (502), the output end of the hydraulic cylinder (502) is fixedly installed with a connecting arm (503) through bolts, the end of the connecting arm (503) is fixedly connected with a mounting sleeve (504), the inside of the mounting sleeve (504) is rotatably connected with a gas supply pipe (505), one end of the bottom of the gas supply pipe (505) extends into the inside of the tank body (1).

2. The device for improving the expression of proteins according to claim 1, characterized in that: The tank body (1) is fixedly connected with an extension plate one (3) on the outer wall of the circumference, the top outer wall of the extension plate one (3) is fixedly installed with a sterile air compressor (4), and the same connecting pipe (506) is installed between the air outlet of the sterile air compressor (4) and the mounting sleeve (504).

3. The device for improving protein expression effect according to claim 1, characterized in that: The tank body (1) is fixedly connected with an extension plate one (3) on the outer wall of the circumference, the top outer wall of the extension plate one (3) is fixedly installed with a sterile air compressor (4), and the same connecting pipe (506) is installed between the air outlet of the sterile air compressor (4) and the mounting sleeve (504).

4. The device for improving protein expression effect according to claim 1, characterized in that: The tank body (1) is fixedly connected with an extension plate one (3) on the outer wall of the circumference, the top outer wall of the extension plate one (3) is fixedly installed with a sterile air compressor (4), and the same connecting pipe (506) is installed between the air outlet of the sterile air compressor (4) and the mounting sleeve (504).

5. The device for improving the expression of a protein according to claim 4, wherein: The bottom of the gas supply pipe (505) is provided with an exhaust assembly (6), the exhaust assembly (6) comprises a gas collecting pipe (601) fixedly installed on the bottom of the gas supply pipe (505), a plurality of horizontally arranged push nozzles one (602) are arranged on one side of the outer wall of the circumference of the gas collecting pipe (601), a plurality of horizontally arranged push nozzles two (603) are arranged on the other side of the outer wall of the circumference of the gas collecting pipe (601), and the push nozzles one (602) and the push nozzles two (603) are oppositely arranged.

6. The device for improving protein expression effect according to claim 1, characterized in that: A plurality of equidistantly distributed air outlet nozzles (604) are arranged on the bottom of the outer wall of the circumference of the gas collecting pipe (601), and a mesh plate is arranged in the outlet of the air outlet nozzle (604).

7. The device for improving protein expression effect according to claim 1, characterized in that: The inside of the gas supply pipe (505) is provided with a second one-way valve. The gas supply pipe (505) and the gas collecting pipe (601) are both made of polypropylene material.

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