Immunochromatography test paper card of dexamethasone
By designing the upper and lower cover structures of the dexamethasone immunochromatographic test strip, the diaphragm and sample dispensing port form a reaction chamber, solving the problems of complex operation, easy leakage, and low sensitivity of existing test strips, and achieving simple and efficient detection results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- CHINA ANIMAL DISEASE CONTROL CENT
- Filing Date
- 2024-12-20
- Publication Date
- 2026-04-10
AI Technical Summary
Existing competitive immunochromatographic test strips suffer from problems such as complex operation procedures, susceptibility to moisture in micropores, high storage requirements, easy leakage, high cost, and low sensitivity.
A dexamethasone immunochromatographic test strip card was designed, which adopts an upper and lower cover structure. The septum and the sample dispensing port form a reaction cell containing gold-labeled antibodies and sealed with a sealing membrane. After the test solution pre-reacts in the reaction cell, it automatically drips onto the test strip and binds to the antigen on the nitrocellulose membrane, simplifying the operation and improving the sensitivity.
It achieves simple operation and high-sensitivity detection, solves the problems of easy leakage in micropores and high storage requirements, reduces costs and improves the applicability of test strips.
Smart Images
Figure CN224109487U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to reagent card technical field, concretely relates to a dexamethasone's immune chromatography test paper card. BACKGROUND
[0002] The working principle of the test paper strip applies the competitive method immune chromatography principle, and the current competitive method immune chromatography test paper strip basically adopts two detection modes: one is two-step microwell strip. It is composed of two parts, and the two parts are in-line microwell and test paper strip. When using, the first step is to drop the measured liquid into the microwell, so that the drug residue in the measured liquid drop and the gold-labeled antibody in the microwell are pre-reacted, and the second step is to drop the pre-reacted measured liquid on the test paper strip, so that the chromatography reaction is carried out. The gold-labeled antibody inhibited in the pre-reaction is combined with the antigen (T line) on the nitrocellulose membrane, and whether the measured substance is contained is judged by affecting the color depth of the T line. The advantages are that ① the antigen and the antibody are pre-reacted, and then the chromatography competition is consumed, ② the reaction is complete, and the sensitivity is high (detection limit: 2 ug / kg), ③ the sample applicability is strong, and the detection requirement of the actual sample can be better met. The disadvantages are that ① the operation process is relatively complex, the measured liquid needs to be pre-reacted with the microwell, ② the microwell (the gold-labeled antibody is contained in the reaction pool, and the pink tablet) is easy to be damp, and the storage environment requirement is high, ③ the microwell is an in-line device, and there are 8 microwells in each row. When a single one is used, the connected microwell package is easy to cause leakage when the microwell package is torn, and detection loss is caused, ④ the microwell cannot be used independently, and the use cost is high; the second one is a one-step gold spraying strip. There is no microwell device, and there is only one test paper strip. When using, the measured liquid is directly spotted on the test paper strip, the measured substance in the sample is combined with the gold-labeled antibody on the gold spraying pad through chromatography, the gold-labeled antibody is inhibited, and then the gold-labeled antibody is combined with the competitive antigen (T line) on the nitrocellulose membrane. Whether the measured substance is contained is judged by affecting the color depth of the T line. The advantage is that the sample is directly spotted, and the operation is simple. The disadvantage is that the chromatography and the competition are synchronized, the combination with the gold-labeled antibody is insufficient, the sensitivity is relatively low (detection limit: 10 ug / kg), and the missed detection situation is easy to occur. CONTENT OF THE UTILITY MODEL
[0003] The utility model aims at providing a dexamethasone's immune chromatography test paper card, and aims at solving the problem of at least one disadvantage in the background art.
[0004] In order to achieve the above-mentioned purpose, the technical scheme adopted by the utility model is as follows:
[0005] A dexamethasone's immune chromatography test paper card, comprising an upper cover, the upper cover is connected with the lower cover, the upper cover is provided with a sample adding port and an observation port along the length direction thereof, the sample adding port is abutted against a diaphragm close to the lower cover end, the diaphragm and the sample adding port form a reaction pool, gold-labeled antibodies are arranged in the reaction pool, and a sealing film is arranged at the end of the sample adding port away from the diaphragm.
[0006] The lower surface of the diaphragm abuts against the sample pad of the test strip.
[0007] The lower cover is provided with clamping mechanisms, and the test strip is arranged between the clamping mechanisms.
[0008] As a limitation, the diaphragm is a water-soluble PVA film.
[0009] As another limitation, the diaphragm is a sustained-release filter membrane.
[0010] As another limitation, the lower cover is provided with a stabilizing mechanism connected to the diaphragm, and the stabilizing mechanism comprises a pair of stabilizing columns arranged on both sides of the test strip.
[0011] As another limitation, the distance between the pair of stabilizing columns is matched with the width of the test strip.
[0012] As another limitation, the stabilizing column is provided with a clamping table near the end of the diaphragm, and the depth of the clamping table is matched with the thickness of the diaphragm.
[0013] As another limitation, the diaphragm is provided with a hanging corner on both sides, and the diaphragm is connected to the stabilizing column when the diaphragm is in the working position.
[0014] As another limitation, the clamping mechanism comprises a pair of end plates and two pairs of side plates, and the pair of end plates are arranged at both ends of the test strip.
[0015] As another limitation, the lower cover is provided with a plurality of support columns, and each support column is arranged on the surface of the lower cover and is close to the edge of the lower cover.
[0016] As another limitation, the lower cover is provided with a plurality of support columns, and each support column is arranged on the surface of the lower cover and is close to the edge of the lower cover.
[0017] Compared with the prior art, the technical progress achieved by the utility model lies in:
[0018] The upper cover is provided with a sample adding port and an observation port at intervals along the length direction, the sample adding port is close to the end of the lower cover and abuts against the diaphragm, the diaphragm and the sample adding port form a reaction pool, the gold-labeled antibody is arranged in the reaction pool, and the sample adding port is provided with a sealing film away from the end of the diaphragm; the problem that the micro-holes are arranged in a row, 8 micro-holes in each row, and the tearing of the micro-hole sealing package easily causes the leakage of the connected micro-hole sealing package and the detection loss when a single micro-hole is used is solved; the target object in the to-be-tested liquid and the gold-labeled antibody are pre-reacted in the micro-hole; the lower surface of the diaphragm abuts against the sample pad of the test paper strip; the lower cover is provided with a clamping mechanism, the test paper strip is arranged between the clamping mechanisms, and the observation line of the test paper strip corresponds to the observation port; after the upper and lower covers are buckled tightly, the gold-labeled antibody is arranged in the reaction pool formed by the sample adding port and the diaphragm, and the sample adding port is sealed by a sealing strip, so that the stability of the gold-labeled antibody is ensured; when in use, the sealing strip is first torn, and then the to-be-tested liquid is dropped into the sample adding port, so that the to-be-tested liquid and the gold-labeled antibody are pre-reacted in the reaction pool formed by the sample adding port and the diaphragm; after the reaction is completed, the to-be-tested liquid automatically drops on the test paper strip under the action of gravity, so as to be combined with the antigen (T line) on the nitrocellulose membrane on the test paper strip, and the T line color development is affected; the advantages of the two detection mode test paper strips of the micro-hole strip and the gold spraying strip are combined, integrated design is carried out, the material and structure of the test paper strip are innovated and optimized, the reaction space similar to the micro-hole device is formed on the test paper strip in the mode of increasing the water-soluble or fiber diaphragm, the test paper strip can be directly sampled without distributed detection, and the operation is simple like the gold spraying card; the reaction space formed on the test paper strip can be used for pre-reaction, the high sensitivity same as the micro-hole strip is obtained, the applicability and practicality of the test paper strip are greatly improved, and finally, the structure is adopted, the reaction pool is formed in single package, and the detection of the to-be-tested liquid is suitable. BRIEF DESCRIPTION OF DRAWINGS
[0019] The accompanying drawings are used to provide a further understanding of the present application, and constitute a part of the specification, and are used to explain the present application together with embodiments of the present application, and do not constitute a limitation on the present application.
[0020] In the drawings:
[0021] Figure 1 It is a structural schematic view of the present application;
[0022] Figure 2 It is a structural schematic view of the upper cover, the sample adding port, the diaphragm, the observation port and the test paper strip of the embodiment of the present application;
[0023] Figure 3 It is a sectional view of the embodiment of the present application; Figure 2
[0024] Figure 4 It is an enlarged structural schematic view of the sample adding port, the diaphragm, the test paper strip and the lower cover of the embodiment of the present application;
[0025] Figure 5 Structure diagram of the upper cover of the embodiment of the present application is shown in the figure;
[0026] Figure 6 Structure diagram of the embodiment of the present application Figure 5 is shown in the figure;
[0027] Figure 7 Structure diagram of the diaphragm of the embodiment of the present application is shown in the figure;
[0028] Figure 8 Structure diagram of the diaphragm, hanging corner and stabilizing column of the embodiment of the present application is shown in the figure;
[0029] Figure 9 Structure diagram of the diaphragm and stabilizing column of the embodiment of the present application is shown in the figure;
[0030] Figure 10 Structure diagram of the stabilizing column, clamping table and lower cover of the embodiment of the present application is shown in the figure.
[0031] Marked components: 1-upper cover, 101-sample adding port, 102-observation port, 2-lower cover, 201-supporting column one, 202-supporting column two, 3-diaphragm, 301-hanging corner, 4-clamping mechanism, 401-end plate, 402-side plate, 5-stabilizing mechanism, 501-stabilizing column, 502-clamping table, 6-test strip, 7-sealing film. DETAILED DESCRIPTION
[0032] The preferred embodiments of the present application are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present application, and are not used to limit the present application.
[0033] The present embodiment discloses an immune chromatography test paper card of dexamethasone, which comprises a test paper strip and a test paper card body. Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6As shown, including the upper cover 1, the upper cover 1 is integrally formed by injection molding, the upper cover 1 can be connected to the lower cover 2 by clamping, the clamping of the upper cover 1 and the lower cover 2 can also use the existing clamping method to fix, the upper cover 1 can also be connected to the lower cover 2 by bonding, the lower cover 2 is integrally formed by injection molding; The upper cover 1 is provided with a sample port 101 and an observation port 102 along the length direction, the observation port 102, the sample port 101 and the lower cover 1 are integrally formed; The sample port 101 is close to the end of the lower cover 2 and abuts against the diaphragm 3, the lower surface of the diaphragm 3 abuts against the sample pad of the test strip 6, the diaphragm 3 can use water-soluble PVA film, the thickness of the water-soluble diaphragm is between 25um-100um, the diaphragm 3 can also use a slow-release filter membrane, such as: glass fiber membrane, the sample port 101 and the diaphragm 3 form a reaction pool, the reaction pool is provided with a gold-labeled antibody, the end of the sample port 101 away from the diaphragm 3 is provided with a sealing film 7, the sealing film 7 uses a medical plastic film, the sealing film 7 can be fixedly connected to the upper cover 1 by hot pressing, the sealing film 7 can also be fixedly connected to the upper cover 1 by bonding, the sealing film 7 can also be fixedly connected to the upper cover 7 by other existing methods; The lower cover 2 is provided with a clamping mechanism 4, the clamping mechanism 4 is provided with a test strip 6, the clamping mechanism 4 can use the existing test strip 6 fixing structure, the sample pad end of the test strip 6 corresponds to the sample port 101, the observation line of the test strip 6 corresponds to the observation port 102, the clamping mechanism 4 can be fixedly connected to the lower cover 2 by hot melting, the lower cover 2 and the clamping mechanism 4 can also be integrally formed; The upper cover 1, the lower cover 2, the test strip 6, the sealing film 7, the gold-labeled antibody and the diaphragm 3 form a complete test paper card; The test paper card can be used for the detection of dexamethasone drug residues, the test strip 6 and the diaphragm 3 are located inside the upper cover 1 and the lower cover 2, after the upper and lower covers are buckled, the gold-labeled antibody (not shown in the gold-labeled antibody diagram) is placed in the reaction pool formed by the sample port 101 and the diaphragm 3, and the sealing film 7 is used to seal the reaction pool at the sample port 101 of the upper cover 1, when packaging, a single test paper card is vacuum packaged (not shown in the vacuum packaging diagram), a desiccant (not shown in the desiccant diagram) is placed in the vacuum packaging, which is convenient for storage; When in use, first, open the package, take out the test paper card, tear open the sealing film 7 on the sample port 101, drop the to-be-tested liquid "such as animal urine containing dexamethasone drug residues" in the sample port 101, so that it reacts with the gold-labeled antibody in the sample port 101 in the reaction pool, after the to-be-tested liquid and the gold-labeled antibody complete the reaction in the reaction pool, the diaphragm 3 is dissolved by the to-be-tested liquid in time or the to-be-tested liquid slowly passes through the diaphragm 3 in time, the to-be-tested liquid will automatically drop on the test strip 6 under the action of gravity, so as to combine with the antigen (T line) on the nitrocellulose membrane on the test strip 6, thereby affecting the color development of the T line, finally, the T line color development of the test strip 6 is observed through the observation port 102; Thus, the advantage of the embodiment is that the above-mentioned setting is adopted, the reaction pool forms a single package, and the stability of the diaphragm is improved.
[0034] As Figure 1 , Figure 3 , Figure 7, Figure 8 , Figure 9 , Figure 10 As shown, the lower cover 2 can be fixedly connected to the stabilizing mechanism 5 by heat fusion. The stabilizing mechanism 5 can also be integrally formed with the lower cover 2. The stabilizing mechanism 5 is connected to the diaphragm 3. The lower surface of the diaphragm 3 abuts against the upper surface of the test strip 6. The setting of the stabilizing mechanism 5 improves the stability of the diaphragm 3. The diaphragm 3 can be directly placed on the upper surface of the stabilizing mechanism 5. The upper surface of the stabilizing mechanism 5 and the upper surface of the test strip are in the same plane. The diaphragm 3 can also be snapped into the stabilizing mechanism 5. The upper surface of the stabilizing mechanism 5 and the upper surface of the diaphragm 3 are in the same plane. The stabilizing mechanism 5 includes a pair of stabilizing posts 501. The pair of stabilizing posts 501 are located on both sides of the test strip 6. The distance between the pair of stabilizing posts 501 is adapted to the width of the test strip 6. There can be one stabilizing post 501 or two stabilizing posts 501. The two stabilizing posts 501 are spaced apart along the length of the test strip 6. The stabilizing column 501 is connected to the lower cover 2 at one end. The end of the stabilizing column 501 near the diaphragm 3 can be provided with a clamping platform 502 through a machine tool. The clamping platform 502 can also be integrally formed with the stabilizing column 501. The depth of the clamping platform 502 is adapted to the thickness of the diaphragm 3. When the diaphragm 3 is in the working position, the four corners of the diaphragm 3 respectively abut against the inner corners of the corresponding clamping platforms 502. In use, the diaphragm 3 is clamped between multiple clamping platforms 502 to prevent the diaphragm 3 from moving forward, backward, left, or right, thereby improving the stability of the diaphragm 3. The diaphragm 3 is provided with hanging corners 301 on both sides. The hanging corners 301 are integrally formed with the diaphragm 3. When the diaphragm 3 is connected to the stabilizing column 501, the hanging corners 301 are located at both ends of the stabilizing column 501. When the diaphragm 3 is in the working position, the diaphragm 3 is connected to the stabilizing column 501 to prevent the diaphragm 3 from moving forward, backward, left, or right, thereby improving the stability of the diaphragm 3.
[0035] like Figure 1 , Figure 3 As shown, the snap-fit mechanism 4 includes a pair of end plates 401 and two pairs of side plates 402. The pair of end plates 401 are located at both ends of the test strip 6, and the two pairs of side plates 402 are located on both sides of the test strip 6. The end plates 401 can be fixedly connected to the lower cover 2 by heat fusion, and the side plates 402 can be fixedly connected to the lower cover 2 by heat fusion. The lower cover 2, end plates 401 and side plates 402 can be integrally formed. The snap-fit mechanism 4 prevents the test strip 6 from moving forward, backward, left and right, thus improving the stability of the test strip 6.
[0036] like Figure 1 , Figure 3As shown, the lower cover 2 is fixedly connected with a plurality of support columns one 201 by hot melting, the support columns one 201 are arranged at intervals along the surface of the lower cover 2, and the support columns one 201 are close to the edge of the lower cover 2; the lower cover 2 is fixedly connected with a plurality of support columns two 202 by hot melting, the support columns two 202 are arranged in a square shape, the square-shaped support columns two 202 correspond to the observation port 102, and the lower cover 2, the support columns one 201 and the support columns two 202 can be integrally formed; when the lower cover 2 is connected with the upper cover 1, the upper ends of the support columns two 202 abut against the lower surface of the upper cover 1, and the stability of the test paper card is improved.
[0037] The working principle of the embodiment of the utility model is as follows:
[0038] In use, first, open the package, take out the test paper card, tear the sealing film 7 on the sample adding port 101, drop the to-be-tested liquid in the sample adding port 101, make it pre-react with the gold-labeled antibody in the sample adding port 101 in the reaction pool, after the to-be-tested liquid and the gold-labeled antibody complete the reaction in the reaction pool, the to-be-tested liquid will timely dissolve the diaphragm 3 or slowly pass through the dissolved diaphragm 3, then automatically drop on the test paper strip 6 under the action of gravity, so as to combine with the antigen (T line) on the nitrocellulose membrane on the test paper strip 6, thereby affecting the T line color development, finally, observe the T line color development of the test paper strip 6 through the observation port 102.
[0039] The above parts not described in detail are all the common knowledge of the person skilled in the art.
[0040] Finally, it should be noted that: the above only describes the preferred embodiments of the utility model, and is not used to limit the utility model, although the utility model is described in detail with reference to the foregoing embodiments, for the person skilled in the art, it still can modify the technical scheme recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model should be included in the protection scope of the utility model claim.
Claims
1. An immunochromatographic test strip card of dexamethasone comprising an upper cover (1) connected to a lower cover (2), characterized in that: The upper cover (1) is provided with a sample adding port (101) and an observation port (102) along the length direction, the sample adding port (101) is close to the end of the lower cover (2) and abuts against the diaphragm (3), the diaphragm (3) and the sample adding port (101) form a reaction pool, gold standard antibodies are arranged in the reaction pool, and the sample adding port (101) is provided with a sealing film (7) away from the diaphragm (3). The lower surface of the diaphragm (3) abuts against the sample pad of the test paper strip (6). The lower cover (2) is provided with a clamping mechanism (4), the test paper strip (6) is arranged between the clamping mechanism (4), and the observation line of the test paper strip (6) corresponds to the observation port (102).
2. The immuno-chromatographic test strip card of dexamethasone according to claim 1, characterized in that: The diaphragm (3) is a water-soluble PVA film.
3. The immuno-chromatographic test strip card of dexamethasone according to claim 1, characterized in that: The diaphragm (3) is a slow-release filter film.
4. The immuno-chromatographic test strip card of dexamethasone according to claim 1, characterized in that: The lower cover (2) is provided with a stabilizing mechanism (5), the stabilizing mechanism (5) is connected with the diaphragm (3), and the stabilizing mechanism (5) comprises a pair of stabilizing columns (501), and the pair of stabilizing columns (501) are respectively located on the two sides of the test paper strip (6).
5. The immunochromatographic test strip card of dexamethasone according to claim 4, characterized by: The distance between the pair of stabilizing columns (501) is matched with the width of the test paper strip (6).
6. The immunochromatographic test strip card of dexamethasone according to claim 5, characterized by: The stabilizing column (501) is provided with a clamping table (502) close to the end of the diaphragm (3), the depth of the clamping table (502) is matched with the thickness of the diaphragm (3), when the diaphragm (3) is in a working position, the four corners of the diaphragm (3) respectively abut against the inner corners of the corresponding clamping tables (502).
7. The immuno-chromatographic test strip card of dexamethasone according to claim 5, characterized by: The diaphragm (3) is provided with a hanging corner (301) on each side, when the diaphragm (3) is in a working position, the diaphragm (3) is connected with the stabilizing column (501).
8. The immuno-chromatographic test strip card of dexamethasone according to claim 1, characterized by: The clamping mechanism (4) comprises a pair of end plates (401) and two pairs of side plates (402), the pair of end plates (401) are respectively located on the two ends of the test paper strip (6), and the two pairs of side plates (402) are respectively located on the two sides of the test paper strip (6).
9. The immunochromatographic test strip card of dexamethasone according to claim 1, characterized by: The lower cover (2) is provided with a plurality of support columns (201), each support column (201) is arranged along the surface of the lower cover (2), and each support column (201) is close to the edge of the lower cover (2).
10. The immunochromatographic test strip card of dexamethasone according to claim 1, characterized by: The lower cover (2) is provided with a plurality of support columns (202), each support column (202) corresponds to the observation port (102), and when the lower cover (2) is connected with the upper cover (1), the upper ends of the support columns (202) respectively abut against the lower surface of the upper cover (1).