Heating and storage module and perfusion device for isolated animal organs

Abstract

The utility model relates to a heating and storage module and a perfusion device for an in-vitro animal organ. The heating and storage module comprises an organ bin, a water bath bin, a heating assembly and a temperature detection module, the organ bin is used for containing in-vitro animal organs and perfusate, so that the in-vitro animal organs are immersed in the perfusate; the water bath bin can store a liquid medium and is detachably accommodated in the organ bin; the heating assembly is arranged at the bottom of the water bath bin and is used for heating the water bath bin, so that the heat is conducted to the organ bin after the liquid medium stored in the water bath bin is heated; the temperature detection module can be used for detecting the temperature of the liquid medium in the water bath bin. The perfusion device comprises the heating and storage module, a perfusion module and a main control module, the perfusion module is used for providing power for perfusate, and the main control module is in communication connection with the heating assembly, the temperature detection module and the perfusion module so as to control the temperature and the perfusion pressure. Compared with the prior art, the integration of the heating module and the storage module is greatly improved, the size is reduced, and the perfusion device can ensure the accuracy of experimental data at an ideal test temperature.

Description

Technical Field

[0001] The embodiments of this utility model relate to a heating device, and more particularly to a heating and storage module and an infusion device for isolated animal organs. Background Technology

[0002] Organ transplantation is the most effective way to treat various end-stage organ diseases. With the development of the human economy and the advancement of science and technology, more and more patients suffering from end-stage organ diseases are joining the transplant list and waiting for organ transplants. Currently, there are more than 1.5 million patients with organ failure in my country each year, and organ transplantation is the best way to save their lives. In recent years, my country's organ transplantation technology has developed rapidly, and the number of organ transplant surgeries performed annually ranks second in the world, indicating a broad market prospect. However, despite the rapid development of transplantation technology and the increasing number of surgeries, the shortage of donor organ resources remains a serious problem. To address the shortage of transplant organs, more and more marginal donor (ECD) organs are being considered for transplantation. However, in conventional organ transplantation surgery, the organ suffers from ischemia-reperfusion injury (IRI) during the entire retrieval and transplantation process. Ischemia-reperfusion injury leads to the activation of a large number of inflammatory cells and the release of a large number of oxygen free radicals, thereby causing damage to the organ. High-quality donor organs can withstand the damage caused by ischemia-reperfusion. However, for marginal standard donors (ECDs), ischemia-reperfusion injury (IRI) is devastating, leading to a series of complications such as allogeneic graft dysfunction (EAD), biliary complications, acute rejection, and primary graft nonfunction (PNF), and even death. Therefore, traditional cold preservation methods are not suitable for marginal donor organs. In recent years, to effectively assess the quality of marginal donor organs in vitro and reduce the risk of complications in transplant patients, mechanical perfusion technology has been continuously developed. Mechanical perfusion (MP) refers to the use of mechanical power to continuously or pulsatilely perfuse the organ's blood vessels and form a circuit, achieving the purpose of in vitro organ preservation. The practical application of mechanical perfusion technology has made ex vivo organ research possible. Currently, normothermic mechanical perfusion technology has been proven to effectively assess organ quality in vitro and reduce postoperative complications in patients.

[0003] The development of normothermic mechanical perfusion technology has made it possible to extend organ preservation time and assess organ quality in vitro. However, many marginal organs (including organs with fatty degeneration, DCD donor organs, organs from aged donors, and infected organs) remain unused and are ultimately discarded. Therefore, utilizing normothermic mechanical perfusion technology to repair damaged organs, expand the donor pool, or treat diseased organs in vitro holds immense scientific and clinical potential. Advancing mechanical perfusion technology and advancing research on the repair and treatment of damaged or diseased organs requires a large number of organ models. Due to the scarcity and preciousness of donor organs, in vitro treatment research cannot be effectively conducted on donor organs. Traditional scientific research is based on cell and animal experiments. Since cells cannot be used for in vitro perfusion research, animal models are the most effective way to expand normothermic mechanical perfusion technology and conduct in vitro research on the repair and treatment of damaged organs. Furthermore, animal normothermic mechanical perfusion systems can not only be applied to transplantation research but also facilitate multidisciplinary research, aiding in drug development, drug screening, genetic engineering, and materials engineering.

[0004] Animal perfusion studies are primarily conducted using pigs, rats, and mice. However, perfusion experiments using pig organs are expensive. Before the experiment begins, the pigs need tracheal intubation, their vital signs need to be maintained via ventilator, and a professional anesthesiologist is required to maintain their vital signs. Organ harvesting also requires a professional surgeon. Furthermore, pigs are expensive to raise. Therefore, using pigs for perfusion experiments consumes significant human, material, and financial resources, making them less than ideal experimental research animals.

[0005] For organ perfusion research, rats and mice are suitable experimental animals. Preliminary verification in any scientific research relies heavily on laboratory mice. Research on rats and mice has a complete scientific system. Furthermore, rats and mice are easy to raise, reproduce quickly, and the surgical procedures are not difficult; two to three researchers can complete the experiment together. However, current small animal perfusion equipment is mainly self-made by researchers, especially the containers used to hold animal organs and the heating devices needed to heat the containers to ensure they are in the testing environment. Because these two types of devices are generally large, and because existing heating devices are difficult to assemble and integrate with organ containers, it is difficult to monitor and control the heating temperature of the heating devices. Utility Model Content

[0006] The purpose of this invention is to design a heating and storage module and infusion device for ex vivo animal organs. This device can not only simulate the physiological environment of various animal organs in vitro, but also integrate the organ container with the heating device, thereby greatly improving the integrity of the heating and storage module and reducing its volume.

[0007] To achieve the above objectives, embodiments of this utility model provide a heating and storage module for isolated animal organs, comprising:

[0008] Organ compartment is used to hold isolated animal organs and perfusion fluid, so that the isolated animal organs are immersed in the perfusion fluid;

[0009] A water bath chamber can store liquid media and can be detachably stored in the organ chamber;

[0010] A heating component is located at the bottom of the water bath chamber and is used to heat the water bath chamber so that the liquid medium stored in the water bath chamber conducts heat to the organ chamber after being heated.

[0011] A temperature detection module is used to detect the temperature of the liquid medium inside the water bath chamber;

[0012] Both the heating component and the temperature detection module are communicatively connected to the main control module of the injection device, enabling the main control module to acquire the temperature measured by the temperature detection module and to control the heating module after acquiring the temperature.

[0013] In addition, embodiments of this utility model also provide a perfusion device for isolated animal organs, comprising:

[0014] The heating and storage module as described above;

[0015] The perfusion module is used to provide power to the perfusion fluid in the organ compartment and to establish extracorporeal circulation for the isolated animal organ.

[0016] The main control module is communicatively connected to each of the heating components, the temperature detection module, and the injection module.

[0017] The main control module is used to acquire the perfusion pressure of the perfusion fluid circulating in the isolated animal organ in real time, and to control the perfusion module after acquiring the perfusion pressure.

[0018] The main control module is also used to acquire the temperature measured by the temperature detection module in real time, and to control the heating component after acquiring the temperature.

[0019] Compared to existing technologies, the heating and storage module of this embodiment not only allows for the placement of isolated animal organs through the organ compartment, but also, because the organ compartment is located within a water bath, and the water bath can be heated by a heating component located at the bottom of the water bath, the integration of the heating and storage module is greatly improved, and its volume is effectively reduced. Furthermore, during the heating process, the temperature of the liquid medium within the water bath can be detected by a temperature detection module. This allows the main control module of the infusion device to effectively control the heating component based on the temperature measured by the temperature detection module. This enables the infusion device to establish extracorporeal circulation of isolated animal organs at ideal experimental temperatures, thus ensuring the accuracy of experimental data. Attached Figure Description

[0020] Figure 1 This is an isometric schematic diagram of the perfusion device for isolated animal organs in some embodiments of this utility model;

[0021] Figure 2 This is a side view schematic diagram of the perfusion device for isolated animal organs in some embodiments of this utility model;

[0022] Figure 3 This is an exploded view of the heating and storage module in some embodiments of the present invention;

[0023] Figure 4 This is a system module block diagram of the perfusion device for isolated animal organs in some embodiments of the present invention;

[0024] Figure 5 This is a top view schematic diagram of the perfusion device for isolated animal organs in some embodiments of the present invention;

[0025] Figure 6 for Figure 5 Sectional view at point AA. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the various embodiments of this utility model will be described in detail below with reference to the accompanying drawings. However, those skilled in the art will understand that many technical details have been presented in the various embodiments of this utility model to enable the reader to better understand this application. However, the technical solutions claimed in this application can be implemented even without these technical details and with various changes and modifications based on the following embodiments.

[0027] Example 1

[0028] Embodiment 1 of this utility model relates to a heating and storage module for isolated animal organs, such as... Figure 3As shown, the heating and storage module 1 includes: organ compartment 11, heating module 12 consisting of water bath compartment 121 and heating component 122, and temperature detection module 13.

[0029] Among them, such as Figure 3 As shown, the organ chamber 11 is used to hold isolated animal organs and perfusion fluid, immersing the isolated animal organs in the perfusion fluid. Secondly, the water bath chamber 121 can store the liquid medium and is detachably stored within the organ chamber 11. Additionally, a heating assembly 122 is located at the bottom of the water bath chamber 121 to heat the water bath chamber 121, allowing the heated liquid medium to transfer heat to the organ chamber 11. Finally, the temperature detection module 13 can be used to detect the temperature of the liquid medium within the water bath chamber 121.

[0030] Furthermore, both the heating component 122 and the temperature detection module 13 are communicatively connected to the main control module 3 of the infusion device. This allows the main control module 3 to acquire the temperature measured by the temperature detection module 13 and control the heating component 122 after obtaining the temperature. For example, if the temperature measured by the temperature detection module 13 is higher than the preset temperature, it indicates that the heating temperature of the organ chamber 11 by the heating module 12 is too high. In this case, the main control module 3 can reduce the heating temperature of the water bath chamber 121 by the heating component 122. Conversely, if the temperature measured by the temperature detection module 13 is lower than the preset temperature, it indicates that the heating temperature of the water bath chamber 121 by the heating component 122 is too low. In this case, the main control module 3 can increase the heating temperature of the water bath chamber 121.

[0031] As can be seen from the above, the heating and storage module of this embodiment not only allows for the placement of isolated animal organs through the organ compartment 11, but also, since the organ compartment 11 is located within the water bath 121 and can be heated by the heating component 122 located at the bottom of the water bath 121, the integration of the heating and storage module is greatly improved, and the volume of the heating and storage module is effectively reduced. Furthermore, during the heating process of the heating component 122, the temperature of the liquid medium inside the water bath 121 can be detected by the temperature detection module. This allows the main control module 3 of the infusion device to effectively control the heating component 122 based on the temperature measured by the temperature detection module 13, enabling the infusion device to establish extracorporeal circulation of isolated animal organs at an ideal experimental temperature, thus ensuring the accuracy of experimental data.

[0032] Furthermore, it is worth mentioning that since the organ compartment 11 is located inside the water bath compartment 121, after the water bath compartment 121 is filled with a liquid medium, such as pure water, the organ compartment 11 can be partially immersed in the liquid medium inside the water bath compartment 121. Thus, when the heating component 122 heats the water bath compartment 121, the heat can be evenly distributed throughout the entire water bath compartment 121 along with the liquid medium and the heat can be conducted to the organ compartment 11 to ensure that the temperature inside the organ compartment 11 can always be kept at a constant temperature.

[0033] It should be noted that, in order for the temperature detection module 13 to effectively detect the heating temperature of the heating component 122 when heating the water bath 121, in some embodiments, such as Figure 3 As shown, the temperature detection module 13 can use a temperature probe, and the temperature probe is used to be inserted into the water bath 121 from the bottom at least partially, so that the temperature probe can detect the temperature of the liquid medium stored in the water bath 121 in real time.

[0034] In addition, in order for the heating assembly 122 to heat the water bath chamber 121, in some other embodiments, such as Figure 3 As shown, the heating assembly 122 includes a heating tray 1221 and an electric heating element 1222. The heating tray 1221 is disposed at the bottom of the water bath 121, and a mounting groove 12211 is provided on one side of the heating tray 1221 opposite to the water bath 121. Additionally, as... Figure 3 As shown, the heating element 1222 is disposed within the mounting groove 12211 of the heating tray 1221 and is tightly fitted to the bottom of the water bath chamber 121. Simultaneously, the heating tray 1221 is also provided with a wire channel 12212, through which the wires 12221 of the heating element 1222 can be led out, allowing the wires 12221 to be electrically connected to the main control module 3 of the infusion device. This enables the main control module 3 to control the heating element 1222 to heat the water bath chamber 121. Furthermore, as a preferred embodiment, in other embodiments, such as... Figure 3As shown, the heating assembly 122 further includes a heat insulation sheet 1223, which is disposed within the mounting groove 12211 of the heating tray 1221. The heat insulation sheet 1223 is located between the heating tray 1221 and the heating element 1222, separating them and preventing heat generated by the heating element 1222 from being conducted to the heating tray 1221. This results in a lower surface temperature for the heating tray 1221, improving the safety of the heating and storage module 1 when heating isolated animal organs. Furthermore, in some embodiments, the heat insulation sheet 1223 can be made of asbestos gaskets to effectively insulate the heating element 1222. In other embodiments, the heat insulation sheet 1223 can be made of other materials; however, this embodiment does not specifically limit the material and type of the heat insulation sheet 1223.

[0035] Furthermore, it should be noted that in other embodiments, such as Figure 5 and Figure 6 As shown, in order to fix the heating and storage module on the outer shell 4 of the filling device, an additional positioning ring 20 can be added. This positioning ring 20 can work with the water bath 121 to clamp the outer shell 4 of the filling device, thereby fixing the entire heating and storage module to the outer shell 4. It is also worth noting that, in order to fix the temperature probe, the positioning ring 20 is also provided with a connection hole 201 for inserting the temperature probe into the water bath 121. A locking component (such as a nut) is used to lock the temperature probe onto the positioning ring 20, thereby enabling the temperature probe to stably detect the temperature of the liquid medium inside the water bath 121.

[0036] Furthermore, in other embodiments, such as Figure 3 As shown, the organ chamber 11 includes: an organ chamber body 111 for holding isolated animal organs and perfusion fluid, and an organ chamber cover 112 for sealing the organ chamber body 111. The organ chamber body 111 has several first notches 1113 along its circumference, and the organ chamber cover 112 has several second notches 1122 along its circumference. The number of first notches 1113 and second notches 1122 are the same and uniquely correspond. Each first notch 1113 communicates with its uniquely corresponding second notch 1122, allowing perfusion tubing to be introduced into the organ chamber body 111, thereby enabling the perfusion device to deliver perfusion fluid to the isolated animal organs.

[0037] Specifically, in some embodiments, such as Figure 3As shown, the organ compartment body 111 includes: a funnel-shaped lower portion 1111 and an annular upper portion 1112. The funnel-shaped lower portion 1111 has an upper edge 11111 and a lower edge 11112 opposite to the upper edge 11111, while the annular upper portion 1112 protrudes from the upper edge 11111 of the funnel-shaped lower portion 1111 in a direction away from the lower edge 11112. Next, as... Figure 3 As shown, the organ compartment cover 112 is fastened to the upper edge 11111 of the funnel-shaped lower part 1111 and engages with the annular upper part 1112, with each first notch 1113 located on the annular upper part 1112. The funnel-shaped lower part 1111 of the organ compartment body 111 can not only hold isolated animal organs but also receive perfusion fluid. At the same time, when the liquid medium in the water bath chamber 121 conducts heat to the organ compartment 11, the funnel-shaped lower part 1111 of the organ compartment body 111 can be immersed in the liquid medium to ensure that the temperature inside the organ compartment 11 remains constant.

[0038] Furthermore, as a preferred embodiment, in other embodiments, such as Figure 3 As shown, the heating and storage module 1 also includes a filter assembly 14, which is detachably disposed within the organ chamber body 111. This filter assembly 14 is used to support the isolated animal organ, and its position along the height of the organ chamber body 111 is adjustable. It is evident that by adjusting the height of the filter assembly 14 within the organ chamber body 111, the depth of organ immersion in the perfusion fluid can be adjusted, thereby better maintaining the temperature of the isolated animal organ and ensuring the accuracy of test data during experiments.

[0039] Furthermore, it is worth noting that, in order to enable the filter assembly 14 to support the isolated animal organ while also adjusting its height within the organ compartment body 111, such as... Figure 3 As shown, the filter assembly 14 includes a filter body (not shown) and a filter clamp 142. The filter body supports the detached animal organ, while the filter clamp 142 has a head end 1421 and a tail end 1422 away from the head end 1421. The filter clamp 142 wraps around the filter body 142 from the head end 1421 to the tail end 1422, supporting and fixing the filter body 142. For example, in some embodiments, such as... Figure 3 As shown, the filter clamp 142 can be an elastic component, and the filter clamp 142 is initially a ring-shaped structure. Simultaneously, the filter clamp 142 can be freely bent under external force, allowing it to elastically engage with the filter body 142 after wrapping around it. Furthermore, corresponding to the head end 1421 and tail end 1422 of the filter clamp 142, as... Figure 3As shown, the filter assembly 14 further includes a first lug 143 and a second lug 144. The first lug 143 is disposed at the head end 1421 of the filter clamp 142, and the second lug 144 is disposed at the tail end 1422 of the filter clamp 142. The first lug 143 and the second lug 144 can also be used to move relative to each other under the action of external force, so that the filter clamp 142 collapses and is adjustable along the height direction of the organ compartment body 111. Conversely, the first lug 143 and the second lug 144 are also used to cause the filter clamp 142 to spring back and engage with the organ compartment body 111 after the external force is released.

[0040] Furthermore, during the prolonged circulation of the perfusion fluid within the isolated animal organ, some of the fluid evaporates due to the heating effect, causing a drop in the fluid level and consequently altering the temperature within the organ chamber and the biochemical parameters of the perfusion fluid. Therefore, as a preferred solution, in some embodiments, such as... Figure 3 As shown, the lower part 1111 of the funnel shape is also provided with an infusion fluid level viewing window 1114, and the infusion fluid level viewing window 1114 extends along the height direction of the organ compartment body 111. Furthermore, corresponding to the position of the infusion fluid level viewing window 1114, as shown... Figure 3 As shown, a water bath level window 123 is also provided on the water bath chamber 121, and the water bath level window 123 extends along the height direction of the water bath chamber 121, so that the perfusion fluid level window 1114 and the water bath level window 123 can be opposite each other. It is easy to see that the perfusion fluid level window 1114 and the water bath level window 123 allow the staff to observe the level of the perfusion fluid in the lower part 1111 of the funnel at any time. This not only ensures that the perfusion fluid can circulate in the isolated animal organ under sterile conditions, but also allows the perfusion fluid level to be observed through the perfusion fluid level window 1114 and the water bath level window 123, so that the experimenters can replenish the perfusion fluid in the organ chamber body 111 in a timely manner, thus ensuring that the perfusion fluid will not be dried up due to evaporation due to excessive temperature.

[0041] Furthermore, in other embodiments, such as Figure 1 and Figure 2 As shown, the heating and storage module 1 also includes a needle holder 15 and a support 17. The needle holder 15 is detachably mounted on the outer wall of the water bath chamber 121, and is combined with... Figure 2 As shown, the needle holder 15 includes: a mounting portion 151 detachably connected to the outer wall of the water bath chamber 121, and a support portion 152 connected to the mounting portion 151. The support portion 152 extends vertically along the height direction of the water bath chamber 121. The support portion 152 is also provided with a plurality of positioning holes 153, and each positioning hole 153 can be arranged along the extending direction of the support portion 152, so that the bracket 17 can be detachably mounted on the support portion 152 of the needle holder 15 through any one or more positioning holes 153. Furthermore, in some other embodiments, such as... Figure 1 and Figure 5 As shown, the heating and storage module 1 also includes a tubing clamp 16 and a gooseneck tube 18. One end of the gooseneck tube 18 is connected to the perfusion tubing, while the other end is detachably fixed to the needle holder 15. The gooseneck tube 18 can be used to reverse the perfusion tubing, thereby changing the direction of the perfusion fluid delivery. This allows the perfusion tubing to better enter the organ compartment body 111 through either the first notch 1113 or the corresponding second notch 1122, ensuring that the perfusion fluid can circulate within the isolated animal organ. Secondly, the tubing clamp 16 is used to clamp and fix the end of the gooseneck tube 18 connected to the perfusion tubing, thus securing the gooseneck tube 18. Furthermore, the bracket 17 can be directly installed and fixed to the support portion 152 of the needle holder 15 using the corresponding positioning holes 153. The bracket 17 can also be used to install the pressure detection module 22, enabling the pressure detection module 22 to stably detect the pressure of the perfusion fluid circulating within the isolated animal organ.

[0042] Example 2

[0043] Embodiment 2 of this utility model relates to an infusion device, such as... Figure 1 and Figure 4 As shown, it includes: a heating and storage module 1, a filling module 2, and a main control module 3 as described in Embodiment 1.

[0044] Among them, combined Figure 3 As shown, the perfusion module 2 is used to provide power to the perfusion fluid contained in the organ compartment 11, so that the perfusion fluid continuously circulates in the isolated animal organ, thereby simulating the extracorporeal circulation of the isolated animal organ.

[0045] In addition, such as Figure 4 As shown, the main control module 3 is communicatively connected to the heating component 122, the temperature detection module 13, and the perfusion module 2. The main control module 3 is used to acquire the perfusion pressure of the perfusion fluid circulating in the isolated animal organ in real time, and to control the perfusion module 2 after acquiring the perfusion pressure. Simultaneously, the main control module 3 is also used to acquire the temperature measured by the temperature detection module 13 in real time, and to control the heating component 122 after acquiring the temperature.

[0046] As can be seen from the above, since the main control module 3 can obtain the perfusion pressure when the perfusion fluid circulates in the isolated animal organ in real time, and the temperature when the heating and storage module 1 heats the isolated animal organ in real time, and controls the perfusion module 2 after obtaining the perfusion pressure, and controls the heating and storage module 1 after obtaining the temperature, it is not necessary for the experimenter to monitor parameters such as perfusion pressure and temperature when establishing extracorporeal circulation of isolated animal organs, thus greatly saving manpower and improving experimental efficiency.

[0047] Specifically, in some embodiments, such as Figure 1 and Figure 4 As shown, the perfusion module 2 includes a peristaltic pump 21 and a pressure detection module 22. The peristaltic pump 21's perfusion tubing (not shown in the figure) is inserted into the heating and receiving module 1, allowing the peristaltic pump 21 to provide power to the perfusion tubing, ensuring continuous circulation of the perfusion fluid within the isolated animal organ. Furthermore, in some embodiments, combined with... Figure 4 As shown, the pressure detection module 22 can employ a pressure sensor, which is used to detect the perfusion pressure when the perfusion fluid circulates in the isolated animal organ. Additionally, as... Figure 4 As shown, the main control module 3 is communicatively connected to the peristaltic pump 21 and the pressure detection module 22. The main control module 3 is used to acquire the infusion pressure measured by the pressure detection module 22. Furthermore, the main control module 3 is used to reduce the rotational speed of the peristaltic pump 21 when the acquired infusion pressure is greater than the target pressure, and to increase the rotational speed of the peristaltic pump 21 when the acquired infusion pressure is less than the target pressure. Alternatively, the main control module 3 is also used to set the rotational speed of the peristaltic pump 21 when delivering the infusion fluid, and to adjust the actual rotational speed of the peristaltic pump 21 according to the set rotational speed, so that the infusion pressure measured by the pressure detection module 22 reaches the target pressure.

[0048] Specifically, the perfusion module 2 can freely switch between two modes when delivering perfusion fluid to isolated animal organs: automatic perfusion mode and manual perfusion mode. In automatic perfusion mode, if the perfusion pressure obtained by the main control module 3 is greater than the target pressure, it indicates that the pressure of the perfusion fluid circulating in the isolated animal organ is too high. In this case, the main control module 3 can reduce the speed of the peristaltic pump 21. Conversely, if the perfusion pressure obtained by the main control module 3 is less than the target pressure, it indicates that the pressure of the perfusion fluid circulating in the isolated animal organ is too low. In this case, the main control module 3 can increase the speed of the peristaltic pump 21. However, in some other embodiments, when the perfusion module 2 delivers perfusion fluid to isolated animal organs using manual perfusion mode, the main control module 3 is also used to set the speed of the peristaltic pump 21 when delivering the perfusion fluid and to adjust the actual speed of the peristaltic pump 21 according to the set speed, so that the perfusion pressure measured by the pressure detection module 22 can reach the target pressure. For example, the rotation speed set by the main control module 3 can be related to the target pressure. For instance, when the main control module 3 adjusts the rotation speed of the peristaltic pump 21, if the pressure measured by the pressure detection module 22 does not reach the target pressure, the operator can manually adjust the rotation speed of the peristaltic pump 21 until the pressure measured by the pressure detection module 22 reaches the target pressure.

[0049] Additionally, it is worth noting that in some embodiments, multiple perfusion modules 2 and heating and storage modules 1 are provided, and each perfusion module 2 and each heating and storage module 1 is communicatively connected to the main control module 3, thereby enabling the main control module 3 to simultaneously establish extracorporeal circulation for multiple isolated animal organs. Furthermore, it should be noted that in other embodiments, such as... Figure 1 As shown, the filling device also includes a housing 4, on which each filling module 2 and each heating and storage module 1 are detachably mounted. Multiple filling modules 2 and multiple heating and storage modules 1 can be integrated and installed through the housing 4, thereby greatly reducing the overall space occupied by the device. Specifically, in order to enable the housing 4 to accommodate the installation of each filling module 2 and each heating and storage module 1, in some embodiments, such as... Figure 1 and Figure 6 As shown, the outer casing 4 is provided with multiple first slots 41, and a positioning ring 20 is provided on the side of the outer casing 4 away from the bottom of the first slot 41. Therefore, when fixing each heating and storage module 1, the water bath 121 of each heating and storage module 1 can cooperate with the positioning ring 20 to clamp the bottom of the first slot 41. At the same time, the water bath 121, the outer casing 4, and the positioning ring 20 are locked by screws 30, so that the entire heating and storage module 1 can be fixedly installed on the outer casing 4. Similarly, corresponding to the fixing method of the outer casing 4 for each heating and storage module 1, in other embodiments, such as Figure 1 As shown, the outer casing 4 can also be provided with multiple second slots 42 for installing each injection module 2, and each second slot 42 can install one injection module 2. At the same time, each second slot 42 is also provided with multiple screw holes (not shown in the figure) around its perimeter. Therefore, when installing each injection module 2, the peristaltic pump 21 can be locked and fixed in the second slot by means of the cooperation between the bolt 23 and the screw holes in the second slot 42, thereby realizing the installation and fixation of each injection module 2 on the outer casing 4.

[0050] Furthermore, it should be noted that since each filling module 2 and each heating and storage module 1 can be integrated and installed through the housing 4, in order to facilitate effective monitoring of the working status of the filling module 2 and each heating and storage module 1, in some other embodiments, such as Figure 1 As shown, the outer casing 4 is also equipped with a display module 5 that is connected to the main control module 3. The display module 5 can be a display screen or other display terminal device. The display module 5 can be used to display the pressure data measured by the pressure detection module 22 and the temperature data measured by the temperature detection module 13, etc., so that the staff can observe at any time and further improve the experimental efficiency.

[0051] Those skilled in the art will understand that the above embodiments are specific implementations of the present invention, and in practical applications, various changes can be made to them in form and detail without departing from the spirit and scope of the present invention.

Claims

1. A heating and storage module for isolated animal organs, characterized in that, include: Organ compartment is used to hold isolated animal organs and perfusion fluid, so that the isolated animal organs are immersed in the perfusion fluid; A water bath chamber, which can store liquid media, is detachably installed inside the organ chamber; A heating component is located at the bottom of the water bath chamber and is used to heat the water bath chamber so that the liquid medium stored in the water bath chamber conducts heat to the organ chamber after being heated. A temperature detection module is used to detect the temperature of the liquid medium inside the water bath chamber; Both the heating component and the temperature detection module are communicatively connected to the main control module of the injection device, enabling the main control module to acquire the temperature measured by the temperature detection module and control the heating component after acquiring the temperature.

2. The heating and housing module for an animal organ ex vivo according to claim 1, wherein, The temperature detection module is a temperature probe, and the temperature probe is used to insert at least partially into the water bath chamber from the bottom, so that the temperature probe can detect the temperature of the liquid medium stored in the water bath chamber in real time.

3. The heating and housing module for an animal organ ex vivo according to claim 2, wherein, The heating component includes: A heating tray is disposed at the bottom of the water bath chamber; wherein, the heating tray has an installation groove on one side opposite to the water bath chamber; An electric heating element is disposed in the mounting groove of the heating tray and is tightly fitted to the bottom of the water bath chamber; wherein, the electric heating element is electrically connected to the main control module through a connecting hole; A heat insulation sheet is disposed in the mounting groove of the heating tray and located between the heating tray and the heating element, separating the heating tray and the heating element.

4. The heating and housing module for an animal organ ex vivo according to claim 2, wherein, The organ storage compartment includes: an organ storage compartment body for holding ex vivo animal organs, and an organ storage compartment cover for sealing the organ storage compartment body; The organ compartment body has several first notches along its circumference, and the organ compartment cover has several second notches along its circumference. The number of first notches and second notches are the same and they correspond uniquely. Each first notch is connected to the uniquely corresponding second notch for the purpose of introducing an infusion pipeline into the organ compartment body.

5. The heating and housing module for an animal organ ex vivo according to claim 4, wherein, The organ storage body includes: A funnel-shaped lower portion; the funnel-shaped lower portion has an upper edge and a lower edge opposite to the upper edge; The annular upper part is formed by the upper edge of the bucket-shaped lower part protruding in a direction away from the lower edge; The organ compartment cover is fastened to the upper edge of the lower part of the funnel shape and engages with the upper part of the ring shape, and each of the first notches is provided on the upper part of the ring shape.

6. The heating and storage module for isolated animal organs according to claim 5, characterized in that, The heating and storage module also includes: A filter assembly is detachably disposed within the organ compartment body for supporting excised animal organs; wherein the position of the filter assembly along the height direction of the organ compartment body is adjustable.

7. The heating and housing module for an animal organ ex vivo according to claim 6, wherein, The filter assembly includes: The filter body is used to support detached animal organs; Filter clamp; the filter clamp has a head end and a tail end away from the head end, and the filter clamp wraps around the filter body from the head end to the tail end to support and fix the filter body; A first lug and a second lug; the first lug is disposed at the head end of the filter clamp, and the second lug is disposed at the tail end of the filter clamp; The first lug and the second lug are used to move relative to each other under the action of external force, so that the filter clamp collapses and is adjustable along the height direction of the organ compartment body; the first lug and the second lug are also used to make the filter clamp spring back and engage with the organ compartment body after the external force is released.

8. The heating and housing module for an animal organ ex vivo according to any one of claims 5-7, wherein, The lower part of the funnel is provided with an infusion fluid level viewing window, which extends along the height direction of the organ compartment body; The water bath chamber is also provided with a water bath level window, which extends along the height direction of the water bath chamber.

9. The heating and housing module for an animal organ ex vivo according to claim 2, wherein, The heating and storage module also includes: A needle holder is detachably mounted on the outer wall of the water bath chamber; the needle holder includes: a mounting part detachably connected to the outer wall of the water bath chamber, and a support part connected to the mounting part; wherein the support part extends vertically along the height direction of the water bath chamber. Gooseneck tube; one end of the gooseneck tube is connected to the infusion line, and the other end is detachably fixed to the needle holder. The gooseneck tube is used for reversing the infusion line. Pipe clamps are used to hold and fix the end of the gooseneck tube that connects to the injection pipeline.

10. A device for perfusion of an animal organ ex vivo, characterized in that, include: Heating and storage module as described in any one of claims 1-9; The perfusion module is used to provide power to the perfusion fluid in the organ compartment, so that the perfusion fluid is continuously circulated in the isolated animal organ. The main control module is communicatively connected to each of the heating components, the temperature detection module, and the injection module. The main control module is used to acquire the perfusion pressure of the perfusion fluid circulating in the isolated animal organ in real time, and to control the perfusion module after acquiring the perfusion pressure. The main control module is also used to acquire the temperature measured by the temperature detection module in real time, and to control the heating component after acquiring the temperature.

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