Cell separating and screening device

Abstract

The utility model belongs to the technical field of biology, and particularly relates to a cell separating and screening device which comprises a device main body, a screening module is mounted in the device main body, a filtering module is arranged above the screening module, and a separating module is mounted at the top of the filtering module; the screening module comprises a main rod, a mounting hole, a fixing disc, a screening plate and a screening groove, the mounting hole is reserved in the front side of the main rod, the fixing disc is arranged on the front side of the mounting hole, the screening plate is mounted on the outer wall of the main rod, and the screening groove is reserved in the end of the screening plate; the device has a multi-screening function, can screen cell clusters for multiple times, classify the cell clusters according to the size and improve the screening effect, has a filtering function, can preliminarily filter separated cell raw materials, filter out and collect the cell raw materials which are not completely separated or do not meet the screening standard, and is provided with two layers of separation fans, so that the separation efficiency is improved. The separation effect can be improved, so that the cell raw materials are separated more thoroughly.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology, specifically a cell separation and screening device. Background Technology

[0002] Cell isolation and screening is a key technology in the fields of biotechnology and cell research. In many research and application scenarios, such as medical research, biopharmaceuticals, and cell therapy, it is necessary to obtain specific types of cells from complex mixed populations of cells. These mixed populations may come from multiple sources such as tissue samples, blood, or cell cultures. Isolating and screening cells from multiple sources facilitates clinical applications.

[0003] Chinese patent CN202222008564.2 discloses a cell separation and screening device, comprising a separator body with an inner cavity. A filter plate and a screening plate are fixedly mounted in the inner cavity. The filter plate is positioned near the top of the inner cavity, and the screening plate is positioned near the bottom. The filter plate and the screening plate sequentially divide the inner cavity from top to bottom into a crushing chamber, a separation and screening chamber, and a storage chamber. The crushing chamber is equipped with a crushing component, the separation and screening chamber is equipped with a pressurizing component, the screening plate is equipped with several screens of different apertures, and the storage chamber contains a conical cavity that cooperates with the several screens. This device features different separation ports and different filter screens at the separation ports, making it easy to operate and integrating crushing, separation, and screening into one unit, facilitating subsequent utilization and research.

[0004] While existing cell separation and screening devices can solve the problem of cell clusters of varying sizes and the potential for contamination during filtration, they offer limited screening levels and cannot perform multi-stage screening, thus reducing the effectiveness of the screening process.

[0005] Therefore, a cell separation and screening device is proposed to address the above problems. Utility Model Content

[0006] The purpose of this invention is to provide a cell separation and screening device to solve the problem mentioned in the background art that the screening level is relatively simple, making it impossible to perform multi-level screening and reducing the screening effect.

[0007] The technical solution adopted by this utility model to solve its technical problem is: a cell separation and screening device, including a device body, a screening module installed inside the device body, a filter module arranged above the screening module, and a separation module installed on the top of the filter module; the screening module includes a main rod, a mounting hole, a fixing plate, a screening plate and a screening groove, a mounting hole is reserved on the front side of the main rod, and a fixing plate is arranged on the front side of the mounting hole, a screening plate is installed on the outer wall of the main rod, and a screening groove is reserved at the end of the screening plate.

[0008] Preferably, the screening module further includes a baffle and an electric rotating shaft, with the baffle provided on the rear side of the main rod, and the electric rotating shaft rotatably connected to the rear side of the baffle.

[0009] Preferably, there are four screening plates, and the screening grooves at the ends of each screening plate are of different diameters.

[0010] Preferably, the main body of the device includes a shell, a support frame and a sealing door, the bottom of the shell is provided with the support frame and the front of the shell is provided with the sealing door.

[0011] Preferably, the filtration module includes a connecting frame, a connecting column, a funnel, and a filter screen. The connecting column is provided above the connecting frame, and the funnel is installed at the bottom of the connecting frame. The outer wall of the funnel is provided with a filter screen.

[0012] Preferably, the separation module includes a feeding box, a through hole, an electric bearing, and a separation fan. The feeding box has a pre-reserved through hole inside, and an electric bearing is installed at the bottom of the feeding box. A separation fan is provided below the electric bearing.

[0013] Preferably, the central axis of the feed box coincides with the central axis of the funnel, and the funnel is connected to the connecting column via a connecting frame to form a detachable structure.

[0014] The advantages of this utility model are:

[0015] 1. This utility model employs the combined operation of the main body of the device and the screening module, which has a multiple screening function. It can screen cell clusters multiple times, classifying them according to size, improving the screening effect, and making the screening results more accurate. It can be equipped with a protective layer, which has better expansion capabilities and improves the practicality of the device. It has a protective effect on the screening module, reducing the interference of the external environment on the screening module during screening. It also has a sealing effect to prevent the leakage of the cell raw materials that need to be screened inside.

[0016] 2. This utility model employs the combined use of a filtration module and a separation module, providing a filtration function. It can perform preliminary filtration on the separated cell raw materials, filtering out and collecting cell raw materials that are not completely separated or do not meet the screening standards, facilitating subsequent separation and improving screening quality. It can perform a separation process on cell raw materials, separating them into cell clusters that need to be screened. The separation fan has two layers, which can improve the separation effect and make the separation of cell raw materials more thorough. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a three-dimensional structural diagram of the entire utility model from the front view;

[0019] Figure 2 This is a three-dimensional structural diagram of the main body of the device of this utility model from the front view;

[0020] Figure 3 This is a schematic diagram of the internal cross-section of the filter module of this utility model;

[0021] Figure 4 This is an exploded view of the screening module of this utility model.

[0022] In the diagram: 1. Main body of the device; 101. Outer shell; 102. Support frame; 103. Sealing door; 2. Screening module; 201. Main rod; 202. Mounting hole; 203. Fixing plate; 204. Screening plate; 205. Screening trough; 206. Baffle; 207. Electric rotating shaft; 3. Filtering module; 301. Connecting frame; 302. Connecting column; 303. Funnel; 304. Filter screen; 4. Separation module; 401. Feed box; 402. Through hole; 403. Electric bearing; 404. Separation fan. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.

[0024] Example 1

[0025] Please see Figures 1 to 4 As shown, a cell separation and screening device includes a main body 1, a screening module 2 installed inside the main body 1, a filter module 3 disposed above the screening module 2, and a separation module 4 installed on top of the filter module 3. The screening module 2 includes a main rod 201, a mounting hole 202, a fixing plate 203, a screening plate 204, and a screening groove 205. The main rod 201 has a pre-drilled mounting hole 202 on its front side, and a fixing plate 203 is disposed on its front side. The fixing plate 203 and the main rod 201 form a detachable structure through the mounting hole 202. A screening plate 205 is installed on the outer wall of the main rod 201. 4. The end of the screening plate 204 is reserved with a screening groove 205. The screening module 2 also includes a baffle 206 and an electric rotating shaft 207. The rear side of the main rod 201 is provided with a baffle 206, and the rear side of the baffle 206 is rotatably connected to the electric rotating shaft 207. There are four screening plates 204 in total, and the screening groove 205 at the end of each screening plate 204 is of different thickness. With this setting, it has a multiple screening function, which can screen cell clusters multiple times, classify them according to size, improve the screening effect, make the screening results more accurate, add a protective layer, expand the capability, and improve the practicality of the device.

[0026] Please see Figures 1 to 2 As shown, the main body 1 of the device includes a shell 101, a support frame 102 and a sealing door 103. The support frame 102 is provided at the bottom of the shell 101, and the sealing door 103 is installed on the front side of the shell 101. The internal space of the shell 101 is circular, and the inner wall of the shell 101 is tightly fitted to the end of the screening plate 204. This arrangement has a protective effect on the screening module 2, reduces the interference of the external environment on the screening module 2 during screening, and also has a sealing effect to prevent leakage of the cell raw materials that need to be screened inside.

[0027] Please see Figure 3 As shown, the filtration module 3 includes a connecting frame 301, a connecting column 302, a funnel 303, and a filter screen 304. The connecting column 302 is located above the connecting frame 301, and the funnel 303 is installed at the bottom of the connecting frame 301. The filter screen 304 is installed on the outer wall of the funnel 303. The filter screen 304 and the funnel 303 are integrated. The funnel 303 and the connecting column 302 form a detachable structure through the connecting frame 301. The central axis of the funnel 303 coincides with the central axis of the separation module 4. With this configuration, it has a filtration function, which can perform preliminary filtration on the separated cell raw materials, filter out and collect the cell raw materials that are not completely separated or do not meet the screening standards, so as to facilitate the next separation and improve the screening quality.

[0028] Please see Figure 3As shown, the separation module 4 includes a feed box 401, a through hole 402, an electric bearing 403, and a separation fan 404. The feed box 401 has a pre-reserved through hole 402, and an electric bearing 403 is installed at the bottom of the feed box 401. The separation fan 404 is located below the electric bearing 403. The separation fan 404 is double-layered, and its diameter is equal to the diameter of the feed box 401. The separation fan 404 is located directly above the funnel 303. The central axis of the feed box 401 coincides with the central axis of the funnel 303. The funnel 303 is connected to the connecting column 302 via a connecting frame 301 to form a detachable structure. With this configuration, the cell raw materials can be separated into cell clusters that need to be screened. The separation fan 404 has two layers, which can improve the separation effect and make the separation of cell raw materials more thorough.

[0029] Working principle: First, the cell raw materials to be separated and screened are fed into the feed box 401 through the through hole 402. The electric bearing 403 drives the separation fan 404 to rotate, breaking up and separating the raw materials. After the cell raw materials are fed into the feed box 401 through the through hole 402, the electric bearing 403 drives the separation fan 404 to perform high-speed shearing separation, ensuring that the cell clusters are fully dissociated. The separated cell raw materials enter the funnel 303 for preliminary filtration, and then enter the main body 1 of the device for screening. The main rod 201 rotates continuously through the electric rotating shaft 207, driving the screening plate 204 to rotate, so that each layer of screening tank 205 performs particle size gradient screening according to the preset pore size, realizing precise grading of cell clusters, screening cell clusters of different sizes into different screening plates 204 to complete the screening work. After screening, the funnel 303 can be removed through the connecting frame 301 to take out the incompletely separated cell raw materials filtered out inside, so as to carry out the next separation and screening work.

[0030] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A cell separation and screening device comprising a device body (1) characterised in that: The main body (1) of the device is equipped with a screening module (2), and a filter module (3) is provided above the screening module (2), and a separation module (4) is installed on the top of the filter module (3). The screening module (2) includes a main rod (201), a mounting hole (202), a fixing plate (203), a screening plate (204), and a screening groove (205). The main rod (201) has a mounting hole (202) reserved on the front side, and a fixing plate (203) is provided on the front side of the mounting hole (202). The screening plate (204) is installed on the outer wall of the main rod (201), and a screening groove (205) is reserved at the end of the screening plate (204).

2. The cell separation and screening device according to claim 1, characterized in that: The screening module (2) also includes a baffle (206) and an electric rotating shaft (207). The baffle (206) is provided on the rear side of the main rod (201), and the electric rotating shaft (207) is rotatably connected to the rear side of the baffle (206).

3. The cell separation and screening device of claim 1, wherein: There are four screening plates (204) in total, and the screening grooves (205) at the ends of each screening plate (204) are of different thicknesses.

4. The cell separation and screening device of claim 1, wherein: The main body (1) of the device includes a shell (101), a support frame (102) and a sealing door (103). The support frame (102) is provided at the bottom of the shell (101), and the sealing door (103) is installed on the front side of the shell (101).

5. The cell separation and screening device of claim 1, wherein: The filter module (3) includes a connecting frame (301), a connecting column (302), a funnel (303) and a filter screen (304). The connecting column (302) is provided above the connecting frame (301), and the funnel (303) is installed at the bottom of the connecting frame (301). The outer wall of the funnel (303) is provided with a filter screen (304).

6. The cell separation and screening device of claim 1, wherein: The separation module (4) includes a feeding box (401), a through hole (402), an electric bearing (403), and a separation fan (404). The feeding box (401) has a through hole (402) inside, and an electric bearing (403) is installed at the bottom of the feeding box (401). A separation fan (404) is provided below the electric bearing (403).

7. A cell separation and screening device according to claim 6, wherein: The central axis of the feed box (401) coincides with the central axis of the funnel (303), and the funnel (303) forms a detachable structure with the connecting frame (301) and the connecting column (302).

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