Microbial sampler intelligent replenishment sampling system and method, and unmanned flight system

Abstract

The application discloses a microbial sampler intelligent liquid supplementing sampling system and method and an unmanned flight system, a control module controls a sampling liquid pump to inject sampling liquid in a sampling liquid bottle into a wet-wall cyclone sampler by a preset liquid injection amount and pauses the sampling liquid pump; the control module controls an air blower to inject air into the wet-wall cyclone sampler for sampling, and adjusts the rotating speed of the air blower in real time according to the flow detected by a sampling flow sensor, so that the sampling flow is maintained within a preset flow range; during the sampling process, a temperature sensor is used for detecting the temperature of the current air environment in real time every second, a humidity sensor is used for synchronously detecting the humidity of the current air environment in real time every second, and the control module is used for substituting the current temperature and the current humidity into a one-second sampling liquid evaporation amount fitting function, calculating the one-second sampling liquid evaporation amount under the current temperature and humidity environment, and controlling the sampling liquid pump to work for a liquid supplementing time, so that the sampling liquid is injected into the wet-wall cyclone sampler to realize intelligent real-time liquid supplementing.

Description

Technical Field

[0001] This invention relates to the field of airborne microbial aerosol technology, and in particular to an airborne microbial aerosol replenishment sampling system, a replenishment sampling method, and an unmanned aerial vehicle (UAV) equipped with the airborne microbial aerosol replenishment sampling system. Background Technology

[0002] Microorganisms from humans, animals, plants, and soil can be distributed into the air through droplets or dust, resulting in a certain variety and quantity of microorganisms in the air. Theoretically, pathogenic microorganisms are generally not present in the air, but aerosols of pathogenic microorganisms are often suspended in the air near hospitals, veterinary clinics, and livestock sheds, and healthy people or animals can often become infected by inhaling them. Air contaminated with pathogenic microorganisms can often become a source or medium of pollution, causing epidemics of infectious diseases. Therefore, airborne microbial testing is of great significance for the prevention and control of infectious diseases, as well as for the hygienic monitoring and protection of the environment.

[0003] The sampling solution in a microbial aerosol sampler will evaporate to some extent under the influence of temperature and humidity. To ensure sampling effectiveness and efficiency, it is necessary to replenish the sampling solution. Current technology generally uses a timed and quantitative replenishment method, but this method is not very accurate in terms of replenishment volume and needs improvement. Summary of the Invention

[0004] This invention addresses the problems and shortcomings of existing technologies by providing an airborne microbial aerosol replenishment sampling system, method, and unmanned aerial vehicle (UAV) equipment.

[0005] The present invention solves the above-mentioned technical problems through the following technical solution:

[0006] This invention provides an intelligent replenishment sampling system for microbial samplers, including a wet-wall cyclone sampler, characterized in that it further includes a temperature sensor, a humidity sensor, a timer, a fan, a sampling flow sensor, a sampling liquid pump, a sample liquid pump, a control module, a sampling liquid bottle, and a sample liquid bottle;

[0007] The control module is used to control the sampling liquid pump to inject the sampling liquid in the sampling liquid bottle into the wet-wall cyclone sampler at a set flow rate and to pause the sampling liquid pump.

[0008] The control module is also used to control the fan to inject air into the wet-wall cyclone sampler for sampling, and to adjust the fan speed in real time according to the flow rate detected by the sampling flow sensor to maintain the sampling flow rate within the preset sampling flow rate range. During the sampling process, the timer is used to detect the current sampling time, the temperature sensor is used to detect the temperature of the current air environment in real time every second, and the humidity sensor is used to detect the humidity of the current air environment in real time every second. The control module is used to substitute the current temperature and current humidity into the fitting function f(x,y) of the sampling liquid evaporation rate per second to calculate the sampling liquid evaporation rate per second under the current temperature and humidity environment, so that the liquid replenishment rate per second is equal to the sampling liquid evaporation rate per second, and to control the sampling liquid pump to work at the set flow rate for the liquid replenishment time and then pause the sampling liquid pump, thereby injecting the sampling liquid into the wet-wall cyclone sampler to achieve intelligent real-time liquid replenishment. The liquid replenishment time = liquid replenishment rate per second / set flow rate.

[0009] The control module is also used to start the sample liquid pump when the timer reaches the preset sampling time, and at the same time control the fan and the sampling liquid pump to stop. The sample liquid pump injects the sample liquid containing microbial aerosol particles collected in the wet-wall cyclone sampler into the sample liquid bottle to realize the sampling of air microbial aerosol particles.

[0010] Preferably, the control module is used to obtain the sampling liquid evaporation amount fitting function f(x,y) under different temperatures, different humidity and different sampling times. The sampling liquid evaporation amount is divided by the corresponding sampling time to obtain the sampling liquid evaporation amount per second. Multiple sets of temperatures and humidity and the corresponding sampling liquid evaporation amounts per second are fitted to obtain the sampling liquid evaporation amount fitting function f(x,y).

[0011] Preferably, the fitting function for the evaporation amount of the sampled liquid per second, f(x,y), is a linear function, f(x,y)=a+bx+cy;

[0012] In the formula, a, b, and c are fitted constants, x is the current temperature, and y is the current humidity.

[0013] Preferably, the connecting tube is equipped with a replenishment flow sensor, and the intelligent replenishment sampling system further includes an alert module; the control module is used to simultaneously activate the replenishment flow sensor when the sample liquid pump is started for replenishment, receive the current replenishment flow detected by the replenishment flow sensor, and control the alert module to issue a warning when the current replenishment flow is zero to remind that the sampling liquid needs to be refilled into the sampling liquid bottle.

[0014] Preferably, the system further includes a housing, and all components contained in the system are integrated within the housing.

[0015] The present invention also provides an unmanned aerial vehicle system, characterized in that it includes an unmanned aerial vehicle and the above-mentioned intelligent replenishment sampling system for microbial samplers, wherein the intelligent replenishment sampling system for microbial samplers is mounted on the unmanned aerial vehicle.

[0016] Preferably, the bottom of opposite sides of the housing is fixed with a locking block, and a placement platform and a drive bay are fixed between the landing gears on both sides of the UAV, with the placement platform located directly above the drive bay;

[0017] The upper surface of the placement platform has a pair of symmetrical inverted L-shaped locking strips fixed on the left and right sides. The pair of inverted L-shaped locking strips are arranged in parallel longitudinally, with the rear end being closed and the front end being open to form a locking groove. The pair of inverted L-shaped locking strips are adapted to the locking blocks on both sides so that the shell can slide longitudinally into the locking groove through the locking blocks. At this time, the locking blocks are exactly in contact with the corresponding inverted L-shaped locking strips. The left and right sides of the placement platform are respectively provided with a left sliding hole and a right sliding hole. The left sliding hole and the right sliding hole are on a horizontal line and are respectively located at the open end of the corresponding side inverted L-shaped locking strip. The placement platform is provided with a left locking strip and a right locking strip to block the open end of the pair of inverted L-shaped locking strips. In the initial position, the left locking strip and the right locking strip are respectively located on both sides of the locking groove.

[0018] The drive chamber is fixed with a drive mechanism for driving the left and right locking bars to move closer to each other to block the open end of the corresponding side-inverted L-shaped locking bar. The left and right ends of the drive mechanism are respectively fixed with a left locking block and a right locking block. The left locking block passes through a left sliding hole and its top is fixed to the left locking bar. The right locking block passes through a right sliding hole and its top is fixed to the right locking bar.

[0019] This invention also provides a method for replenishing and sampling airborne microbial aerosols, characterized by comprising the following steps:

[0020] S1. The control module controls the sampling liquid pump to inject the sampling liquid in the sampling liquid bottle into the wet-wall cyclone sampler at a set flow rate and then pauses the sampling liquid pump.

[0021] S2. The control module controls the fan to inject air into the wet-wall cyclone sampler for sampling, and adjusts the fan speed in real time according to the flow rate detected by the sampling flow sensor to maintain the sampling flow rate within the preset sampling flow rate range. During the sampling process, the timer is used to detect the current sampling time, the temperature sensor is used to detect the current air temperature in real time every second, and the humidity sensor is used to detect the current air humidity in real time every second. The control module is used to substitute the current temperature and current humidity into the fitting function f(x,y) of the sampling liquid evaporation rate per second to calculate the sampling liquid evaporation rate per second under the current temperature and humidity environment, so that the liquid replenishment rate per second is equal to the sampling liquid evaporation rate per second, and controls the sampling liquid pump to work at the set flow rate for the liquid replenishment time and then pauses the sampling liquid pump, thereby injecting the sampling liquid into the wet-wall cyclone sampler to achieve intelligent real-time liquid replenishment. The liquid replenishment time = liquid replenishment rate per second / set flow rate.

[0022] S3. When the timer reaches the preset sampling time, the control module controls the sample liquid pump to start and simultaneously controls the fan and sampling liquid pump to stop. The sample liquid pump injects the sample liquid containing microbial aerosol particles collected in the wet-wall cyclone sampler into the sample liquid bottle, thereby realizing the sampling of airborne microbial aerosol particles.

[0023] Based on common knowledge in the field, the above-mentioned preferred conditions can be combined arbitrarily to obtain various preferred embodiments of the present invention.

[0024] The positive and progressive effects of this invention are as follows:

[0025] The sampling liquid in the sampling bottle of this invention evaporates with the sampling airflow and is strongly correlated with the temperature and humidity of the current air environment. The evaporation amount of the sampling liquid corresponding to different temperatures and humidity is found and fitted to obtain a fitting function f(x,y) for the evaporation amount of the sampling liquid per second. This function is used to perform intelligent liquid replenishment through calculation. Its main function is to replenish the liquid in the wet-wall cyclone sampler to achieve the effect of long-term sampling. After the sampling is completed, the sample liquid of the microbial aerosol particles collected in the wet-wall cyclone sampler is injected into the sample bottle through the sample liquid pump to realize the sampling of air microbial aerosol particles.

[0026] This invention facilitates the quick installation and fixation of the intelligent liquid replenishment sampling system for microbial samplers onto a drone, and also facilitates the quick removal of the intelligent liquid replenishment sampling system for microbial samplers from the drone, thus enabling rapid assembly and disassembly between the intelligent liquid replenishment sampling system for microbial samplers and the drone. Attached Figure Description

[0027] Figure 1-2 This is a control principle diagram of the intelligent replenishment sampling system for microbial samplers according to a preferred embodiment of the present invention.

[0028] Figure 3This is a schematic diagram of the intelligent fluid replenishment and sampling system of the present invention mounted on a drone, according to a preferred embodiment of the present invention.

[0029] Figure 4-5 This is a schematic diagram of the drive mechanism according to a preferred embodiment of the present invention.

[0030] Figure 6 This is a schematic diagram of the sampling liquid evaporation fitting in a preferred embodiment of the present invention. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. Terms indicating positional relationships, such as "middle," "horizontal," "vertical," "longitudinal," "front," "back," "left," "right," "inner," and "outer," are based on the positional relationships shown in the accompanying drawings and do not imply that the components referred to must be presented in the described positional relationships, nor do they constitute a limitation on the technical solutions of this invention.

[0033] like Figure 1-2 As shown, this embodiment of the invention provides an intelligent liquid replenishment sampling system 200 for microbial samplers, including a housing 201. The housing 201 integrates a wet-wall cyclone sampler 202, a temperature sensor 203, a humidity sensor 204, a timer 205, a fan 206, a sampling flow sensor 207, a sampling liquid pump 208, a sample liquid pump 209, a control module 210, a sampling liquid bottle 211, and a sample liquid bottle 212.

[0034] The control module 210 is used to control the sampling liquid pump 208 to inject the sampling liquid in the sampling liquid bottle 211 into the wet-wall cyclone sampler 202 at a set flow rate and to pause the sampling liquid pump 208.

[0035] The control module 210 is also used to control the fan 206 to inject air into the wet-wall cyclone sampler 202 for sampling, and to adjust the speed of the fan 206 in real time according to the flow rate detected by the sampling flow sensor 207, so that the sampling flow rate is maintained within the preset sampling flow rate range (e.g., 300L / min ± 2ml). During the sampling process, the timer 205 is used to detect the current sampling time, the temperature sensor 203 is used to detect the temperature of the current air environment in real time every second, and the humidity sensor 204 is used to detect the humidity of the current air environment in real time every second. The control module 210 is used to substitute the current temperature and current humidity into the fitting function f(x,y) of the sampling liquid evaporation rate per second, calculate the sampling liquid evaporation rate per second under the current temperature and humidity environment, make the liquid replenishment rate per second equal to the sampling liquid evaporation rate per second, and control the sampling liquid pump to work at the set flow rate for the liquid replenishment time and then pause the sampling liquid pump, thereby injecting the sampling liquid into the wet-wall cyclone sampler to achieve intelligent real-time liquid replenishment. The liquid replenishment time = liquid replenishment rate per second / set flow rate.

[0036] The control module 210 is also used to start the sample liquid pump 209 when the timer 205 reaches the preset sampling time, and at the same time control the fan 206 and the sampling liquid pump 208 to stop. The sample liquid pump 209 injects the sample liquid containing microbial aerosol particles collected in the wet-wall cyclone sampler 202 into the sample liquid bottle 212 to realize the sampling of air microbial aerosol particles.

[0037] Among them, the fitting function f(x,y) for the evaporation of the sampled liquid in one second is obtained: The control module is used to obtain the evaporation of the sampled liquid under different temperatures, different humidity and different sampling times (see Table 1). The evaporation of the sampled liquid is divided by the corresponding sampling time to obtain the evaporation of the sampled liquid in one second. Multiple sets of temperatures and humidity and the corresponding evaporation of the sampled liquid in one second are fitted to obtain the fitting function f(x,y) for the evaporation of the sampled liquid in one second.

[0038] Specifically, the fitting function f(x,y) for the evaporation rate of the sampled liquid per second is a linear function (see...). Figure 6 The formula is f(x,y)=a+bx+cy; where a, b, and c are fitted constants, x is the current temperature, and y is the current humidity. After fitting, the optimal values ​​for a are 0.1753, b is 0.0887, and c is 0.003922.

[0039] Table 1

[0040]

[0041] In this embodiment, intelligent real-time liquid replenishment can be achieved based on changes in ambient temperature and humidity, with the replenishment volume error controllable to <±2ml; the sampling liquid injection volume can be set with an error of <±0.5ml; and liquid replenishment and liquid extraction (removal to sample liquid bottle 212) are controlled separately to prevent cross-contamination.

[0042] Furthermore, a replenishment flow sensor 213 is installed on the connecting pipe, and a reminder module 214 (such as an alarm indicator light) is also provided on the housing 201; the control module 210 is used to simultaneously start the replenishment flow sensor 213 when the sample liquid pump 209 is started to replenish the liquid, receive the current replenishment flow detected by the replenishment flow sensor 213, and control the reminder module 214 to issue a warning when the current replenishment flow is zero, so as to remind that the sampling liquid needs to be added to the sampling liquid bottle 211.

[0043] like Figure 3-5 As shown, this embodiment of the invention also provides an unmanned aerial vehicle system, which includes a drone 100 and the above-mentioned intelligent liquid replenishment sampling system 200 for microbial samplers, wherein the intelligent liquid replenishment sampling system 200 for microbial samplers is mounted on the drone 100.

[0044] The specific loading method is as follows: the bottom of the opposite sides of the housing 201 is fixed with a locking block 214, and the landing gear 101 on both sides of the UAV 100 is fixed with a placement platform 1 and a drive bay 2, with the placement platform 1 located directly above the drive bay 2.

[0045] To achieve a stable connection between the intelligent liquid replenishment sampling system 200 for microbial samplers and the placement platform 1, a pair of symmetrical inverted L-shaped clips 3 are fixed on the left and right sides of the upper surface of the placement platform 1. The pair of inverted L-shaped clips 3 are arranged in parallel longitudinally, with the rear end of the pair of inverted L-shaped clips 3 being closed and the front end being open to form a slot. The slot constitutes the placement position of the intelligent liquid replenishment sampling system 200 for microbial samplers. The pair of inverted L-shaped clips 3 are adapted to the clips 214 set at the bottom positions on both sides of the intelligent liquid replenishment sampling system 200 for microbial samplers, so that the housing 201 slides longitudinally into the pair of inverted L-shaped clips 3 (i.e., the slot) through the clips 214. At this time, the clips 214 are exactly in contact with the corresponding inverted L-shaped clips 3. The placement platform 1 has a left sliding hole 4 and a right sliding hole 5 on its left and right sides, respectively. The left sliding hole 4 and the right sliding hole 5 are on the same horizontal line and are located at the open ends of the corresponding inverted L-shaped card strips. The left sliding hole 4 extends to the right to the left side of the placement position of the intelligent liquid replenishment sampling system 200 for the microbial sampler, and the right sliding hole 5 extends to the left to the right side of the placement position of the intelligent liquid replenishment sampling system 200 for the microbial sampler. A left locking strip 6 and a right locking strip 7 are horizontally slidably connected on the placement platform 1 to block the open ends of the pair of inverted L-shaped card strips 3. In the initial position, the left locking strip 6 and the right locking strip 7 are located on both sides of the card slot, and the size of the left locking strip 6 and the right locking strip 7 is not smaller than the size of the open end of the corresponding inverted L-shaped card strip 3.

[0046] A drive mechanism is fixed on the drive compartment 2. This mechanism drives the left locking strip 6 and right locking strip 7 to move closer together to block the open ends of the corresponding side-tilted L-shaped locking strip 3, thereby firmly fixing the intelligent liquid replenishment sampling system 200 of the microbial sampler onto the drone 100. The drive mechanism also drives the left locking strip 6 and right locking strip 7 to move further apart from each other, so as not to block the open ends of the corresponding side-tilted L-shaped locking strip 3, facilitating the removal of the intelligent liquid replenishment sampling system 200 of the microbial sampler from the drone 100. This drive mechanism and blocking method enable rapid assembly and disassembly of the intelligent liquid replenishment sampling system 200 of the microbial sampler from the drone 100.

[0047] The drive mechanism includes a drive shaft 8, a gear 9, a left rack 10, a right rack 11, and a drive knob 12. The drive shaft 8 is longitudinally arranged inside the drive chamber 2 and rotatably connected to the inner wall of the rear side plate of the drive chamber 2. The gear 9 is fixed on the drive shaft 8. The right rack 11 and the left rack 10 are distributed vertically on the upper and lower sides of the gear 9 and mesh with the gear 9. A left locking block 13 is fixed on the upper surface of the left end of the left rack 10, and a right locking block 14 is fixed on the upper surface of the right end of the right rack 11. The left locking block 13 passes through a left sliding hole 4 and its top is fixed to a left locking bar 6. The right locking block 14 passes through a right sliding hole 5 and its top is fixed to a right locking bar 7. The drive knob 12 is arranged on the outside of the drive chamber 2 and is longitudinally slidably connected to the drive shaft 8, so that the drive knob 12 can slide back and forth relative to the drive shaft 8 and the drive shaft 8 and the drive knob 12 cannot rotate relative to each other.

[0048] The method for quickly installing and fixing the intelligent fluid replenishment sampling system 200 of the microbial sampler onto the drone 100 is as follows: the operator rotates the drive knob 12, and under the rotation of the drive knob 12, the drive shaft 8 rotates accordingly. Through the cooperation of the gear 9 with the left rack 10 and the right rack 11, the left locking bar 6 and the right locking bar 7 move closer to each other and move to the open end of the corresponding side-inverted L-shaped locking bar 3 to block the open end of the inverted L-shaped locking bar 3, thereby fixing the intelligent fluid replenishment sampling system 200 of the microbial sampler onto the drone 100.

[0049] The method for quickly removing the intelligent liquid replenishment sampling system 200 of the microbial sampler from the drone 100 is as follows: The operator rotates the drive knob 12 in the opposite direction. Driven by the reverse rotation of the drive knob 12, the drive shaft 8 rotates in the opposite direction. Through the cooperation of the gear 9 with the left rack 10 and the right rack 11, the left locking bar 6 and the right locking bar 7 are driven to move away from each other, so that the left locking bar 6 and the right locking bar 7 do not block the open end of the inverted L-shaped locking bar 3. Then, the intelligent liquid replenishment sampling system 200 of the microbial sampler is pulled out longitudinally, thereby quickly removing the intelligent liquid replenishment sampling system 200 of the microbial sampler from the drone 100.

[0050] To lock the drive knob 12, a locking block 15 is fixed to the drive knob 12. The outer wall of the front side plate 21 of the drive compartment 2 has a placement groove 16 for placing the drive knob 12 and a locking groove 17 for the locking block 15 to be inserted and engaged. When closing the open end of the inverted L-shaped locking strip 3, simply control the drive knob 12 to rotate at a suitable angle so that the locking block 15 is aligned with the locking groove 17, and then push the drive knob 12 towards the front side plate 21, that is, push the drive knob 12 backward so that the locking block 15 is inserted into the locking groove 17, thereby locking the drive knob 12. Conversely, unlocking the drive knob 12 is achieved by moving it backward.

[0051] To further ensure the connection stability between the locking block 15 and the locking groove 17, magnets that attract each other are fixed on the back of the locking block 15 and the inner bottom of the locking groove 17.

[0052] To facilitate operation and prevent slippage, the drive knob 12 has a ring of anti-slip texture along the circumference, making it easy for operators to grip and rotate.

[0053] This invention also provides a method for replenishing and sampling airborne microbial aerosols, comprising the following steps:

[0054] S1. The control module 210 controls the sampling liquid pump 208 to inject the sampling liquid in the sampling liquid bottle 211 into the wet-wall cyclone sampler 202 at a set flow rate and then pauses the sampling liquid pump 208.

[0055] S2. The control module 210 controls the fan 206 to inject air into the wet-wall cyclone sampler 202 for sampling, and adjusts the speed of the fan 206 in real time according to the flow rate detected by the sampling flow sensor 207 to maintain the sampling flow rate within the preset sampling flow rate range. During the sampling process, the timer 205 is used to detect the current sampling time, the temperature sensor 203 is used to detect the current air temperature in real time every second, and the humidity sensor 204 is used to detect the current air humidity in real time every second. The control module 210 substitutes the current temperature and current humidity into the fitting function f(x,y) of the sampling liquid evaporation rate per second to calculate the sampling liquid evaporation rate per second under the current temperature and humidity environment, so that the liquid replenishment rate per second is equal to the sampling liquid evaporation rate per second, and controls the sampling liquid pump 208 to work at the set flow rate for the liquid replenishment time and then pauses the sampling liquid pump, thereby injecting the sampling liquid into the wet-wall cyclone sampler 202 to achieve intelligent real-time liquid replenishment. The liquid replenishment time = liquid replenishment rate per second / set flow rate.

[0056] S3. When the timer 205 reaches the preset sampling time, the control module 210 controls the sample liquid pump 209 to start, and simultaneously controls the fan 206 and the sampling liquid pump 208 to stop. The sample liquid pump 209 injects the sample liquid containing microbial aerosol particles collected in the wet-wall cyclone sampler 202 into the sample liquid bottle 212, thereby realizing the sampling of airborne microbial aerosol particles.

[0057] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. A smart replenishment sampling system for microbial samplers, comprising a wet-wall cyclone sampler, characterized in that, It also includes a temperature sensor, humidity sensor, timer, fan, sampling flow sensor, sampling liquid pump, sample liquid pump, control module, sampling liquid bottle and sample liquid bottle; The control module is used to control the sampling liquid pump to inject the sampling liquid in the sampling liquid bottle into the wet-wall cyclone sampler at a set flow rate and to pause the sampling liquid pump. The control module is also used to control the fan to inject air into the wet-wall cyclone sampler for sampling, and to adjust the fan speed in real time according to the flow rate detected by the sampling flow sensor so that the sampling flow rate is maintained within the preset sampling flow rate range. During the sampling process, the timer is used to detect the current sampling time, the temperature sensor is used to detect the current air temperature in real time every second, the humidity sensor is used to detect the current air humidity in real time every second, and the control module is used to substitute the current temperature and current humidity into the fitting function f(x,y) of the sampling liquid evaporation rate per second to calculate the sampling liquid evaporation rate per second under the current temperature and humidity environment, so that the liquid replenishment rate per second is equal to the sampling liquid evaporation rate per second, and control the sampling liquid pump to work at the set flow rate for the liquid replenishment time and then pause the sampling liquid pump, thereby injecting the sampling liquid into the wet-wall cyclone sampler to achieve intelligent real-time liquid replenishment. The liquid replenishment time = liquid replenishment rate per second / set flow rate; The control module is also used to start the sample liquid pump when the timer reaches the preset sampling time, and at the same time control the fan and the sampling liquid pump to stop. The sample liquid pump injects the sample liquid containing microbial aerosol particles collected in the wet-wall cyclone sampler into the sample liquid bottle to realize the sampling of air microbial aerosol particles.

2. The intelligent fluid replenishment sampling system for microbial samplers as described in claim 1, characterized in that, Obtain the fitting function f(x,y) for the evaporation rate of the sampled liquid per second: The control module is used to obtain the evaporation amount of the sampled liquid under different temperatures, humidity and sampling times. The evaporation amount of the sampled liquid is divided by the corresponding sampling time to obtain the evaporation amount of the sampled liquid per second. Multiple sets of temperatures and humidity and the corresponding evaporation amounts of the sampled liquid per second are fitted to obtain the fitting function f(x,y) for the evaporation amount of the sampled liquid per second.

3. The intelligent fluid replenishment sampling system for microbial samplers as described in claim 2, characterized in that, The fitting function for the evaporation amount of the sampled liquid in one second, f(x,y), is a linear function, f(x,y)=a+bx+cy; In the formula, a, b, and c are fitted constants, x is the current temperature, and y is the current humidity.

4. The intelligent replenishment sampling system for microbial samplers as described in claim 2, characterized in that, The connecting tube is equipped with a replenishment flow sensor, and the intelligent replenishment sampling system also includes an alert module; The control module is used to simultaneously activate the replenishment flow sensor when the sample liquid pump is started to replenish the sample liquid, receive the current replenishment flow detected by the replenishment flow sensor, and control the reminder module to issue a warning when the current replenishment flow is zero, so as to remind the sample liquid bottle to be refilled with sample liquid.

5. The intelligent replenishment sampling system for microbial samplers as described in any one of claims 1-4, characterized in that, The system also includes a housing, and all the components contained in the system are integrated inside the housing.

6. An unmanned aerial vehicle system, characterized in that, It includes a drone and the intelligent fluid replenishment sampling system for microbial samplers as described in claim 5, wherein the intelligent fluid replenishment sampling system for microbial samplers is mounted on the drone.

7. The unmanned aerial system as described in claim 6, characterized in that, The bottom of the opposite sides of the housing is fixed with a locking block, and a placement platform and a drive bay are fixed between the landing gears on both sides of the drone. The placement platform is located directly above the drive bay. The upper surface of the placement platform has a pair of symmetrical inverted L-shaped locking strips fixed on the left and right sides. The pair of inverted L-shaped locking strips are arranged in parallel longitudinally, with the rear end being closed and the front end being open to form a locking groove. The pair of inverted L-shaped locking strips are adapted to the locking blocks on both sides so that the shell can slide longitudinally into the locking groove through the locking blocks. At this time, the locking blocks are exactly in contact with the corresponding inverted L-shaped locking strips. The left and right sides of the placement platform are respectively provided with a left sliding hole and a right sliding hole. The left sliding hole and the right sliding hole are on a horizontal line and are respectively located at the open end of the corresponding side inverted L-shaped locking strip. The placement platform is provided with a left locking strip and a right locking strip to block the open end of the pair of inverted L-shaped locking strips. In the initial position, the left locking strip and the right locking strip are respectively located on both sides of the locking groove. The drive chamber is fixed with a drive mechanism for driving the left and right locking bars to move closer to each other to block the open end of the corresponding side-inverted L-shaped locking bar. The left and right ends of the drive mechanism are respectively fixed with a left locking block and a right locking block. The left locking block passes through a left sliding hole and its top is fixed to the left locking bar. The right locking block passes through a right sliding hole and its top is fixed to the right locking bar.

8. The unmanned aerial system as described in claim 7, characterized in that, The drive mechanism includes a drive shaft, a gear, a left rack, a right rack, and a drive knob. The drive shaft is longitudinally arranged inside the drive compartment and rotatably connected to the inner wall of the rear side plate of the drive compartment. A gear is fixed on the drive shaft. The right rack and the left rack are distributed vertically on the upper and lower sides of the gear and mesh with the gear. A left locking block is fixed on the upper surface of the left end of the left rack, and a right locking block is fixed on the upper surface of the right end of the right rack. The drive knob is arranged outside the drive compartment and is longitudinally slidably connected to the drive shaft, so that the drive knob can slide back and forth relative to the drive shaft but cannot rotate relative to it. Driven by the rotation of the drive knob, the drive shaft rotates accordingly. Through the cooperation of the gears with the left and right racks, the left and right locking bars are driven to move closer to each other and to the open end of the corresponding inverted L-shaped locking bar to seal the open end of the inverted L-shaped locking bar.

9. The unmanned aerial system as described in claim 8, characterized in that, A locking block is fixed on the drive knob, and a placement groove for placing the drive knob and a locking groove for the locking block to be inserted and engaged are provided on the outer wall of the front side plate of the drive compartment.

10. The unmanned aerial system as described in claim 9, characterized in that, Magnets that attract each other are fixed to the back of the locking block and the inner bottom of the locking groove.

11. The unmanned aerial system as described in claim 7, characterized in that, The dimensions of the left and right locking bars are not less than the dimensions of the open ends of the corresponding side-inverted L-shaped locking bars; The left sliding hole extends to the right to the left side of the placement position of the microbial aerosol sampler, and the right sliding hole extends to the left to the right side of the placement position of the microbial aerosol sampler.

12. A method for replenishing fluid in airborne microbial aerosol sampling, characterized in that, Includes the following steps: S1. The control module controls the sampling liquid pump to inject the sampling liquid in the sampling liquid bottle into the wet-wall cyclone sampler at a set flow rate and then pauses the sampling liquid pump. S2. The control module controls the fan to inject air into the wet-wall cyclone sampler for sampling, and adjusts the fan speed in real time according to the flow rate detected by the sampling flow sensor to keep the sampling flow rate within the preset sampling flow rate range. During the sampling process, a timer is used to detect the current sampling time, a temperature sensor is used to detect the current air temperature in real time every second, and a humidity sensor is used to detect the current air humidity in real time every second. The control module is used to substitute the current temperature and current humidity into the fitting function f(x,y) of the sampling liquid evaporation rate per second, calculate the sampling liquid evaporation rate per second under the current temperature and humidity environment, make the liquid replenishment rate per second equal to the sampling liquid evaporation rate per second, and control the sampling liquid pump to work at the set flow rate for the liquid replenishment time and then pause the sampling liquid pump, thereby injecting the sampling liquid into the wet-wall cyclone sampler to achieve intelligent real-time liquid replenishment. The liquid replenishment time = liquid replenishment rate per second / set flow rate. S3. When the timer reaches the preset sampling time, the control module controls the sample liquid pump to start and simultaneously controls the fan and sampling liquid pump to stop. The sample liquid pump injects the sample liquid containing microbial aerosol particles collected in the wet-wall cyclone sampler into the sample liquid bottle, thereby realizing the sampling of airborne microbial aerosol particles.

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