Rapid detection device for antibody and antigen

Abstract

The rapid antibody and antigen detection device comprises a box shell and a drawer structure, a lead screw sliding table is assembled on the inner top face of the box shell, and a near-field fluorescence detection module and a light sensing piece are fixedly installed on the bottom face and the inner side face of a sliding base of the lead screw sliding table respectively. A plurality of photoelectric sensors are transversely erected on the back face of the inner side of the box shell, and a display module electrically connected with the near-field fluorescence detection module is arranged on the outer top face of the box shell. According to the rapid detection device for the antibody and the antigen, a drawer structure is arranged, a multi-channel containing structure can be provided for batch fluorescence immunochromatography kits, automatic continuous detection can be achieved by means of cooperation of a near-field fluorescence detection module driven by a lead screw sliding table and an external display module, the detection efficiency is effectively improved, and meanwhile, the detection accuracy is improved. The closed detection cavity formed by the enclosure of the box shell and the drawer structure not only meets the accurate detection requirement of a laboratory, but also supports an outdoor mobile detection scene.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a rapid detection device for antibodies and antigens. Background Technology

[0002] Antibody and antigen detection are core means of disease diagnosis, food safety monitoring and environmental pollutant screening. Their technology has undergone multiple generations of innovation. Traditional gold standard methods such as enzyme-linked immunosorbent assay (ELISA) achieve highly sensitive quantitative detection (detection limit can reach pg / mL level) through enzyme-catalyzed colorimetric reaction. However, they have inherent defects such as complicated operation, long incubation time (usually 4-6 hours) and dependence on laboratory environment, which make it difficult to meet the needs of point-of-care testing (POCT).

[0003] In the 21st century, immunochromatography technology has given rise to rapid test strip products. Based on the principle of antigen-antibody specific binding, these products use colloidal gold or latex particle markers to achieve visualized qualitative or semi-quantitative results, reducing the time for a single test to within 15 minutes. However, this technology has low sensitivity (usually only at the ng / mL level) and the results depend on visual interpretation, making them susceptible to subjective factors.

[0004] To overcome the sensitivity bottleneck and achieve accurate quantification, fluorescence immunochromatography has emerged. This technology uses fluorescent labels and, combined with a dedicated reading instrument, can significantly improve detection sensitivity (to the pg / mL level), while achieving digital quantitative detection through peak wavelength analysis.

[0005] In the process of realizing this invention, the inventors discovered at least the following problems in the prior art:

[0006] While traditional enclosed instruments can eliminate ambient light interference through built-in light shields and filters, their bulky structure limits on-site mobility. Currently available fluorescence readers are portable, but most adopt a single-channel sample introduction design, requiring manual insertion of each test strip, which is difficult to adapt to high-throughput scenarios (such as sample screening during infectious disease outbreaks). There are also some handheld, near-field detection devices or smartphone-adapted devices that can directly read the fluorescence signal on the test strip. Although it does not require the test strip to be placed in the instrument, it is still necessary to bring the test strip close to the instrument's detection window. Furthermore, the exposed detection window is prone to baseline noise in strong or diffused light environments, leading to quantitative errors.

[0007] Therefore, the aforementioned technical problems need to be solved. Utility Model Content

[0008] To overcome the shortcomings of existing technologies, this invention proposes a rapid detection device for antibodies and antigens, which solves the problem of irreconcilable technical contradictions in existing fluorescence immunochromatographic quantitative detection equipment in terms of sampling throughput, ambient light interference resistance, and on-site mobility.

[0009] To solve the above-mentioned technical problems, the basic technical solution proposed by this utility model is as follows:

[0010] A rapid detection device for antibodies and antigens includes a housing and a drawer structure for accommodating several fluorescent immunochromatographic reagent kits arranged side-by-side at equal intervals. The drawer structure covers the opening of the housing and encloses a detection chamber for accommodating the several fluorescent immunochromatographic reagent kits.

[0011] The inner top surface of the housing is equipped with a lead screw slide distributed along its length, and a near-field fluorescence detection module and a photosensitive sheet are fixedly installed on the bottom and inner sides of the lead screw slide. Several photoelectric sensors adapted to the photosensitive sheet are horizontally mounted on the back side of the inner side of the housing, and several fluorescence immunochromatographic reagent kits correspond one-to-one with several photoelectric sensors. A display module electrically connected to the near-field fluorescence detection module is provided on the outer top surface of the housing.

[0012] Preferably, the device further includes a mounting base for assembling several photoelectric sensors. Each photoelectric sensor is fixedly mounted on the mounting base by two first bolts. The mounting base has two transversely formed T-slots for the sliding heads of the first bolts. The design of the mounting base facilitates the convenient installation of a batch of photoelectric sensors, while the connection structure of the T-slots and first bolts facilitates the adjustment of the photoelectric sensor positions.

[0013] Preferably, the drawer structure includes two bases and a support platform fixedly mounted on top of the two bases along a horizontal plane, and a panel covering the outside of the two bases and the support platform. Two clamping assemblies, both arranged laterally and centrally symmetrically, are installed through the support platform. A receiving cavity is formed between the two bases and the support platform for installing the two clamping assemblies.

[0014] The clamping assembly includes a rod and several baffles, each fixedly mounted on the rod at equal intervals and extending through a support platform. One end of the rod movably passes through one of the bases and has an end cap. A return spring, coaxially sleeved on the outside of the rod, is fixedly connected between the end cap and the base. The support platform has several square holes arranged in double rows, staggered to allow the baffles to move laterally. Traditional positioning structures, such as grooves or partitions, are only suitable for positioning and installing fluorescence immunochromatographic reagent kits of a single size. However, commercially available fluorescence immunochromatographic reagent kits come in various sizes. Preferably, by providing two clamping assemblies, the pair of baffles can clamp and fix fluorescence immunochromatographic reagent kits of different widths under the return spring's reset action.

[0015] Preferably, the device further includes a limiting frame laterally mounted on the rear of the top surface of the support platform via two second bolts. The support platform has two slotted holes, both distributed along the width of the platform, through which the second bolts can be fitted. The limiting frame is used to limit one end of the fluorescence immunochromatographic reagent kit. In conjunction with the slotted holes and the second bolts, the position of the limiting frame can be adjusted for different sizes of fluorescence immunochromatographic reagent kits to ensure that the observation window of the reagent kit is always within the specified area, thus ensuring that the near-field fluorescence detection module corresponds to the observation window of the reagent kit.

[0016] Preferably, the panel also includes several magnetic blocks arranged in a rectangular array and embedded on the inner side of the panel. The panel covers the opening of the housing, thus forming a closed detection cavity. The magnetic blocks ensure a tight seal between the panel and the housing, improving the stability of the detection.

[0017] The beneficial effects of this utility model are:

[0018] The technical solution of this utility model, by setting up a drawer structure, can provide a multi-channel carrying structure for batch fluorescence immunochromatographic reagent kits. With the help of a near-field fluorescence detection module driven by a lead screw slide and an external display module, automated continuous detection can be achieved. Compared with traditional single-channel equipment, it significantly improves the throughput of a single detection and effectively improves the detection efficiency. It is especially suitable for rapid screening needs in emergency scenarios such as infectious disease outbreaks. At the same time, the closed detection cavity formed by the shell and the drawer structure creates a physical shielding layer, effectively eliminating the interference of environmental scattered light and improving the accuracy and stability of quantitative detection. In addition, the modular shell design not only meets the precise detection needs of the laboratory, but also supports outdoor mobile detection scenarios, ensuring the portability of the equipment. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the structure of the housing of this utility model;

[0021] Figure 3 This is a schematic diagram of the structure of the lead screw slide of this utility model;

[0022] Figure 4 This is a schematic diagram of the structure of the mounting base of this utility model;

[0023] Figure 5 This is a schematic diagram of the pull-out drawer structure of this utility model;

[0024] Figure 6 This is a schematic diagram of the clamping assembly of this utility model;

[0025] Figure 7 This is a schematic diagram of the structure of the support platform of this utility model;

[0026] Explanation of reference numerals in the attached figures:

[0027] 100. Box shell;

[0028] 110. Lead screw slide; 120. Near-field fluorescence detection module; 130. Photosensitive film; 140. Photoelectric sensor; 150. Display module; 160. Mounting base; 170. First bolt;

[0029] 1610, T-slot;

[0030] 200. Pull-out drawer structure;

[0031] 210. Base; 220. Support platform; 230. Panel; 240. Clamping assembly; 250. Second bolt; 260. Limiting frame; 270. Magnetic block;

[0032] 2210, square hole; 2220, strip hole;

[0033] 2410, Rod; 2420, Baffle; 2430, End; 2440, Return spring. Detailed Implementation

[0034] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0035] Please see Figure 1-7 This utility model provides a technical solution: a rapid detection device for antibodies and antigens, including a housing 100 and a drawer structure 200 for accommodating a number of fluorescent immunochromatographic reagent kits arranged side by side at equal intervals. The drawer structure 200 can cover the opening of the housing 100 and enclose a detection cavity for accommodating the number of fluorescent immunochromatographic reagent kits. The inner top surface of the housing 100 is equipped with a lead screw slide 110 distributed along its length direction, and a near-field fluorescence detection module 120 and a photosensitive sheet 130 are fixedly installed on the bottom and inner sides of the lead screw slide 110 slide seat, respectively. A number of photoelectric sensors 140 adapted to the photosensitive sheet 130 are horizontally mounted on the back side of the inner side of the housing 100, and the number of fluorescent immunochromatographic reagent kits correspond one-to-one with the number of photoelectric sensors 140. A display module 150 electrically connected to the near-field fluorescence detection module 120 is provided on the outer top surface of the housing 100.

[0036] Based on the above structural configuration, the rapid antibody and antigen detection device consists of a housing 100 and a pull-out drawer structure 200. The pull-out drawer structure 200 is used for loading batches of fluorescent immunochromatographic reagent kits. The specific positioning structure is not shown in the accompanying drawings, but the structure can vary, such as grooves for recessed installation of the fluorescent immunochromatographic reagent kits, or partition structures for side-mounted mounting of the kits. After the pull-out drawer structure 200, loaded with batches of fluorescent immunochromatographic reagent kits, is inserted into the housing 100, it forms a closed detection chamber. The screw slide 110, near-field fluorescence detection module 120, display module 150, photosensor 130, and multiple photoelectric sensors 1 are mounted on the housing 100. The 40 configuration enables automated continuous detection of batches of fluorescent immunochromatographic reagent kits within the detection chamber. Specifically, during operation, the operator loads multiple fluorescent immunochromatographic reagent kits into the drawer structure 200 and inserts the drawer structure 200 into the housing 100, creating a closed detection environment. The operator then starts the equipment, causing the slide of the lead screw stage 110 to move the near-field fluorescence detection module 120 laterally. When the detection window of the near-field fluorescence detection module 120 moves above a fluorescent immunochromatographic reagent kit, the photoelectric sensor 140 corresponding to that kit detects the photosensitive film 130, the lead screw stage 110 pauses, and the near-field fluorescence detection module 120 reads the fluorescence immunochromatographic reagent. The fluorescent signal on the kit is displayed in real time via the display module 150. Then, the lead screw slide 110 resumes its movement and continues to move the near-field fluorescence detection module 120 horizontally via its slide block until the next photoelectric sensor 140 detects the photosensitive sheet 130. Thus, with the coordinated operation of the photosensitive sheet 130 and the photoelectric sensor 140, each fluorescent immunochromatographic reagent kit placed in the detection chamber can be detected sequentially. The detection results are displayed and recorded on the display module 150. After completing a single batch detection, the slide block of the lead screw slide 110 automatically returns to its initial position. The operator can quickly replace the kit with a new batch via the drawer structure 200. This rapid antibody and antigen detection device, with its drawer structure 200, can provide batch detection... The quantitative fluorescence immunochromatographic assay kit offers a multi-channel carrying structure. With the help of a near-field fluorescence detection module 120 driven by a lead screw slide 110 and an external display module 150, automated continuous detection can be achieved. Compared with traditional single-channel devices, it significantly improves the throughput of a single detection and effectively enhances detection efficiency. It is especially suitable for rapid screening needs in emergency scenarios such as infectious disease outbreaks. At the same time, the closed detection chamber formed by the housing 100 and the drawer structure 200 creates a physical shielding layer, effectively eliminating the interference of ambient scattered light and improving the accuracy and stability of quantitative detection. In addition, the modular housing 100 design meets the needs of precise laboratory detection and supports outdoor mobile detection scenarios, ensuring the portability of the device.

[0037] Furthermore, it also includes a mounting base 160 for assembling a number of photoelectric sensors 140. The number of photoelectric sensors 140 are all locked and fixed on the mounting base 160 by two first bolts 170. The mounting base 160 has two T-slots 1610 laterally provided for the sliding accommodation of the heads of the first bolts 170.

[0038] Furthermore, the drawer structure 200 includes two bases 210 and a support platform 220 fixedly mounted on the top of the two bases 210 along a horizontal plane, and a panel 230 covering the outside of the two bases 210 and the support platform 220. Two clamping assemblies 240, both arranged laterally and centrally symmetrically, are installed through the support platform 220. A receiving cavity is formed between the two bases 210 and the support platform 220 for installing the two clamping assemblies 240.

[0039] The clamping assembly 240 includes a rod 2410 and several baffles 2420 that are fixedly mounted on the rod 2410, arranged at equal intervals, and pass through the support platform 220. One end of the rod 2410 is movably passed through one of the bases 210 and has an end head 2430. A return spring 2440 is fixedly connected between the end head 2430 and the base 210 and is coaxially sleeved on the outside of the rod 2410. The support platform 220 has several square holes 2210 arranged in a double row to allow the baffles 2420 to move laterally.

[0040] Furthermore, it also includes a limiting frame 260 that is laterally mounted on the rear of the top surface of the support platform 220 by two second bolts 250. The support platform 220 has two strip holes 2220 that are distributed along the width of the support platform 220 and can be adapted to pass through the second bolts 250.

[0041] Furthermore, it also includes several magnetic blocks 270 arranged in a rectangular array and embedded on the inner side of the panel 230.

[0042] Based on the disclosure and teachings of the above specification, those skilled in the art can make changes and modifications to the above embodiments. Therefore, this utility model is not limited to the specific embodiments disclosed and described above, and some modifications and changes to this utility model should also fall within the protection scope of the claims of this utility model. Furthermore, although some specific terms are used in this specification, these terms are only for convenience of explanation and do not constitute any limitation on this utility model.

Claims

1. A rapid detection device for antibodies and antigens, characterized in that: It includes a housing (100) and a drawer structure (200) that can accommodate several fluorescent immunochromatographic reagent kits arranged side by side at equal intervals. The drawer structure (200) can cover the opening inserted into the housing (100) and enclose a detection cavity that can accommodate several fluorescent immunochromatographic reagent kits. The inner top surface of the housing (100) is equipped with a lead screw slide (110) distributed along its length direction, and a near-field fluorescence detection module (120) and a photosensitive sheet (130) are fixedly installed on the bottom and inner sides of the lead screw slide (110), respectively. Several photoelectric sensors (140) adapted to the photosensitive sheet (130) are horizontally mounted on the back side of the inner side of the housing (100), and several fluorescence immunochromatographic reagent kits correspond one-to-one with several photoelectric sensors (140) in front and back. A display module (150) electrically connected to the near-field fluorescence detection module (120) is provided on the outer top surface of the housing (100).

2. The rapid detection device of an antibody and antigen according to claim 1, characterized in that: It also includes a mounting base (160) for assembling a plurality of photoelectric sensors (140), wherein the plurality of photoelectric sensors (140) are fixedly mounted on the mounting base (160) by two first bolts (170), and the mounting base (160) has two T-slots (1610) laterally provided for the sliding of the heads of the first bolts (170).

3. The rapid detection device for antibody and antigen according to claim 1, characterized in that: The drawer structure (200) includes two bases (210) and a support platform (220) fixedly mounted on the top of the two bases (210) along the horizontal plane, and a panel (230) covering the outside of the two bases (210) and the support platform (220). Two clamping components (240) are installed through the support platform (220), both arranged laterally and centrally symmetrically. A receiving cavity is formed between the two bases (210) and the support platform (220) for the two clamping components (240) to be installed. The clamping assembly (240) includes a rod (2410) and several baffles (2420) that are fixedly mounted on the rod (2410) and arranged at equal intervals and pass through the support platform (220). One end of the rod (2410) is movably passed through one of the bases (210) and has an end head (2430) at the end. A return spring (2440) is fixedly connected between the end head (2430) and the base (210) and is coaxially sleeved on the outside of the rod (2410). Several square holes (2210) arranged in double rows and staggered are provided on the support platform (220) to allow the baffles (2420) to move laterally.

4. The rapid detection device for antibody and antigen according to claim 3, characterized in that: It also includes a limiting frame (260) that is horizontally mounted on the rear of the top surface of the support platform (220) by two second bolts (250). The support platform (220) has two strip holes (2220) that are distributed along the width direction of the support platform (220) and can be fitted through by the second bolts (250).

5. The rapid detection device for antibody and antigen according to claim 3, characterized in that: It also includes several magnetic blocks (270) arranged in a rectangular array and embedded on the inner side of the panel (230).

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