Liquid bag structure and micro-fluidic detection chip

Abstract

The embodiment of the utility model provides a liquid bag structure and a micro-fluidic detection chip, and relates to the technical field of micro-fluidic chips, and the liquid bag structure comprises a substrate which is provided with a plurality of grooves arranged at intervals; the puncturing structures are arranged in all the grooves; the liquid bag layer is installed on one side of the base plate, the liquid bag layer is provided with a plurality of liquid bag units, each liquid bag unit is provided with a liquid bag cavity, and each liquid bag cavity at least corresponds to one puncturing structure in position. In the liquid bag structure and the microfluidic detection chip, a liquid bag layer is provided with a plurality of liquid bag units, and each liquid bag unit is provided with a liquid bag cavity. During use, the liquid bag unit is pushed to move towards one side close to the puncturing structure, and the puncturing structure can be used for puncturing into the liquid bag cavity, so that a reagent in the liquid bag cavity flows out. In the process, the reagent is not in contact with the outside, so that the condition that the reagent is polluted by pollutants in the environment can be effectively reduced.

Description

Technical Field

[0001] This application relates to the field of microfluidic chip technology, and in particular to a liquid bladder structure and a microfluidic detection chip. Background Technology

[0002] In related technologies, microfluidic chips, also known as lab-on-a-chip technologies, are... Microfluidic chip technology offers advantages such as small sample volume, rapid analysis speed, ease of manufacturing into portable instruments, and suitability for point-of-care and on-site analysis, and has been widely applied in many fields including biology, chemistry, and medicine. The combination of microfluidic chips with in vitro diagnostic reagents and technologies has led to the development of numerous rapid detection technologies, such as microfluidic-based colloidal gold immunoassay, immunofluorescence assay, chemiluminescence assay, and microfluidic-based nucleic acid assay.

[0003] Current nucleic acid testing reagents require manual solution preparation, a cumbersome process. Furthermore, reagent dispensing is performed in an open environment, making them susceptible to contamination from external pollutants, which compromises the accuracy of experimental results. Utility Model Content

[0004] This application aims to address at least one of the technical problems existing in the prior art. To this end, this application proposes a liquid-filled capsule structure and a microfluidic detection chip, which can simplify the preparation process of detection reagents during nucleic acid detection and reduce the risk of reagent contamination.

[0005] An embodiment of the first aspect of this application provides a liquid bladder structure, including:

[0006] The substrate has multiple spaced grooves;

[0007] A piercing structure is provided in each of the grooves;

[0008] A liquid bladder layer is mounted on one side of the substrate. The liquid bladder layer has a plurality of liquid bladder units, each of the liquid bladder units having a liquid bladder cavity, and each of the liquid bladder cavities corresponding to at least one of the puncture structures.

[0009] The puncturing structure is used to puncture the fluid sac cavity located on one side thereon, and there is a gap between the end of the puncturing structure and the fluid sac cavity.

[0010] Furthermore, the puncture structure includes a conical portion, and the substrate has a liquid outlet hole on one side corresponding to the conical portion.

[0011] Furthermore, the liquid bladder unit includes a liquid bladder shell and a liquid bladder sealing membrane. A liquid bladder cavity with an open end is formed on the liquid bladder shell, and the liquid bladder sealing membrane is sealed at the opening of the liquid bladder cavity to enclose the closed liquid bladder cavity.

[0012] Furthermore, the fluid-filled cavity is hemispherical.

[0013] Furthermore, it also includes an adhesive layer disposed between the liquid bladder layer and the substrate.

[0014] Furthermore, the adhesive layer has through holes at the positions corresponding to the liquid bladder cavity.

[0015] Furthermore, it also includes a liquid bladder cover, which is disposed on the outer periphery of the liquid bladder layer, wherein the liquid bladder cover has a window provided at the position corresponding to the liquid bladder cavity.

[0016] Furthermore, the liquid bladder cover is provided with a first snap-fit ​​structure, and the substrate is provided with a second snap-fit ​​structure, wherein the first snap-fit ​​structure and the second snap-fit ​​structure are connected in cooperation.

[0017] Furthermore, the liquid bladder includes a limiting portion that abuts against three sides of the substrate.

[0018] An embodiment of the second aspect of this application provides a microfluidic detection chip, including the liquid sac structure as described above.

[0019] As can be seen from the above technical solutions, the embodiments of this application have at least the following beneficial effects:

[0020] In the liquid sac structure and microfluidic detection chip provided in this application embodiment, the liquid sac layer is provided with multiple liquid sac units, each of which has a liquid sac cavity for storing reagents. During use, by pushing the liquid sac unit towards the side closest to the puncture structure, the puncture structure can be used to puncture the liquid sac cavity, allowing the reagent inside to flow out. During this process, the reagent does not come into contact with the outside, effectively reducing the possibility of contamination by environmental pollutants. Simultaneously, the gap between the puncture structure and the liquid sac cavity effectively reduces the possibility of accidental puncture of the liquid sac cavity. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the structure of a microfluidic detection chip provided in one embodiment of this application;

[0023] Figure 2 This is an exploded view of a microfluidic detection chip provided in one embodiment of this application.

[0024] Figure 3 This is a top view of a microfluidic detection chip provided in one embodiment of this application;

[0025] Figure 4 for Figure 3 A schematic diagram of the cross-sectional structure of AA in the middle section;

[0026] Figure 5 This is a schematic diagram of the substrate structure in one embodiment of this application;

[0027] Figure 6 for Figure 5 A partially enlarged structural diagram of section B;

[0028] Figure 7 This is a schematic diagram of the structure of the liquid bladder in one embodiment of this application.

[0029] Figure label:

[0030] 100, substrate; 110, groove; 120, second snap-fit ​​structure; 130, liquid outlet hole;

[0031] 200. Piercing structure; 210. Conical part; 211. Depressed area;

[0032] 300. Fluid-filled sac layer; 310. Fluid-filled sac unit; 311. Fluid-filled sac cavity;

[0033] 400, Adhesive layer; 410, Through-hole;

[0034] 500, Liquid bladder cover; 510, Window; 520, First snap-fit ​​structure; 530, Limiting part. Detailed Implementation

[0035] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0036] In related technologies, microfluidic chips, also known as lab-on-a-chip technologies, are... Microfluidic chip technology offers advantages such as small sample volume, rapid analysis speed, ease of manufacturing into portable instruments, and suitability for point-of-care and on-site analysis, and has been widely applied in many fields including biology, chemistry, and medicine. The combination of microfluidic chips with in vitro diagnostic reagents and technologies has led to the development of numerous rapid detection technologies, such as microfluidic-based colloidal gold immunoassay, immunofluorescence assay, chemiluminescence assay, and microfluidic-based nucleic acid assay.

[0037] Current nucleic acid testing reagents require manual solution preparation, a cumbersome process. Furthermore, reagent dispensing is performed in an open environment, making them susceptible to contamination from external pollutants, which can affect the accuracy of experimental results.

[0038] Based on this, this application proposes a liquid bladder structure and a microfluidic detection chip to effectively solve the aforementioned problems.

[0039] See Figure 1 and Figure 2 As shown, an embodiment of the first aspect of this application discloses a liquid bladder structure, including a substrate 100, a puncture structure 200, and a liquid bladder layer 300.

[0040] Specifically, the substrate 100 has a plurality of spaced grooves 110; each groove 110 is provided with a piercing structure 200; the liquid bladder layer 300 is mounted on one side of the substrate 100, the liquid bladder layer 300 has a plurality of liquid bladder units 310, each liquid bladder unit 310 has an independent and closed liquid bladder cavity 311, and each liquid bladder cavity 311 corresponds to at least one position of a piercing structure 200;

[0041] The puncture structure 200 is used to puncture the fluid sac cavity 311 located on one side of it. There is a gap between the end of the puncture structure 200 and the fluid sac cavity 311, which can effectively reduce the possibility of the fluid sac cavity 311 being accidentally punctured.

[0042] It is worth understanding that in the embodiments of this application, the liquid bladder 311 can be used to store reagents or other forms of fluid, such as gases, liquid metals and other types of fluids.

[0043] In the liquid sac structure provided in this embodiment, the liquid sac layer 300 is provided with multiple liquid sac units 310, each liquid sac unit 310 having a liquid sac cavity 311, which can be used to store reagents. In use, by pushing the liquid sac unit 310 toward the side closer to the puncture structure 200, the puncture structure 200 can be used to puncture the liquid sac cavity 311, causing the reagent inside the liquid sac cavity 311 to flow out. During this process, the reagent does not come into contact with the outside, effectively reducing the possibility of contamination of the reagent by environmental pollutants.

[0044] In existing solutions, some microfluidic chips also use a piercing cone on the wall of a fan-shaped orifice to puncture the liquid sac by contacting the piercing cone. See patent application CN118638621A. However, in this solution, the piercing cone is placed on the orifice wall, and the tip of the piercing cone does not point towards the liquid sac, resulting in poor puncture effect.

[0045] In some embodiments of this application, see Figure 2 and Figure 4The puncture structure 200 includes a conical portion 210, and a liquid outlet hole 130 is provided on one side of the substrate 100 corresponding to the conical portion 210. The tip of the conical portion 210 is positioned towards the liquid bladder cavity 311, and the cross-sectional area of ​​the conical portion 210 gradually increases from the tip to the root. In this way, when the conical portion 210 punctures the liquid bladder cavity 311, it can gradually puncture and tear the liquid bladder cavity 311, facilitating the outflow of reagents from the liquid bladder cavity 311.

[0046] In one possible implementation, see Figures 3 to 6 The conical portion 210 has a recessed region 211, the outline of which is conical. When the conical portion 210 is inserted into the liquid sac cavity 311, the outer periphery of the conical portion 210 abuts against the liquid sac cavity 311, and the recessed region 211 creates a gap between the liquid sac cavity 311 and the conical portion 210, thereby facilitating the outflow of reagents from the liquid sac cavity 311.

[0047] Furthermore, a liquid outlet hole 130 is provided on the substrate 100 corresponding to the recessed area 211, thereby facilitating the outflow of reagents from the liquid bladder cavity 311 through the liquid outlet hole 130.

[0048] In the embodiments of this application, the bottom wall of the groove 110 on the substrate 100 is arc-shaped, and the piercing structure 200 is located at the lowest point of the bottom wall of the groove 110, which facilitates the full discharge of reagents.

[0049] In this embodiment, see Figure 4 The liquid outlet 130 is located at the lowest point of the groove 110, which facilitates the complete discharge of reagent from the liquid bladder cavity 311 through the groove 110. The groove is arc-shaped, which can serve to collect the reagent.

[0050] In some embodiments of this application, see Figure 2 and Figure 4 The liquid bladder unit 310 includes a liquid bladder shell and a liquid bladder sealing membrane. A liquid bladder cavity 311 with an open end is formed on the liquid bladder shell. The liquid bladder sealing membrane is sealed at the opening of the liquid bladder cavity 311 to enclose the closed liquid bladder cavity 311. During assembly, the liquid bladder sealing membrane is attached to the end face of the substrate 100, and the opening of the liquid bladder cavity 311 is directly opposite the opening of the groove 110 on the substrate 100. At this time, the puncture structure 200 is positioned towards the liquid bladder sealing membrane, and there is a gap between the puncture structure 200 and the liquid bladder sealing membrane. When the liquid bladder shell is pushed towards the puncture structure 200, the puncture structure 200 punctures the liquid bladder sealing membrane, allowing the reagent stored in the liquid bladder cavity 311 to flow into the groove 110.

[0051] In one possible implementation, the liquid bladder shell is composed of a membrane structure.

[0052] In some embodiments of this application, see Figure 4The liquid sac 311 is hemispherical to facilitate the complete drainage of the reagent inside the liquid sac 311.

[0053] In some embodiments of this application, the liquid bladder structure includes an adhesive layer 400 disposed between the liquid bladder layer 300 and the substrate 100. The adhesive layer 400 is used to connect the liquid bladder layer 300 and the substrate 100, so that the liquid bladder layer 300 is fixed on the substrate 100.

[0054] In this embodiment, see Figure 4 The liquid bladder unit, corresponding to the outer periphery of the opening of the liquid bladder cavity 311, is sealed to the substrate 100 via an adhesive layer 400. This ensures the airtightness of the space enclosed by the liquid bladder shell and the groove 110 of the substrate 100. In practical applications, squeezing the liquid bladder unit allows the puncture structure 200 to puncture the liquid bladder sealing film, simultaneously pushing the reagent inside the liquid bladder cavity 311 out through the outlet hole 130 and flowing to the next cavity.

[0055] In some embodiments of this application, the adhesive layer 400 is provided with a through hole 410 at the position corresponding to the liquid bladder cavity 311. In this way, when the puncture structure 200 pierces the liquid bladder sealing membrane, the influence of the adhesive layer 400 can be reduced, and the resistance to puncturing the liquid bladder sealing membrane can be reduced.

[0056] In some embodiments of this application, the liquid bladder structure further includes a liquid bladder cover 500, which covers the outer periphery of the liquid bladder layer 300. The liquid bladder cover 500 has a window 510 positioned corresponding to the liquid bladder cavity 311. In this embodiment, the liquid bladder cover 500 protects the liquid bladder layer 300, reducing or preventing deformation of the liquid bladder layer 300 under external pressure during packaging and transportation. This effectively prevents the liquid bladder sealing film of the liquid bladder layer 300 from contacting the puncture structure 200 below, thus avoiding leakage caused by the puncture structure 200 damaging the integrity of the liquid bladder sealing film under continuous stress.

[0057] In the above embodiment, the liquid bladder cover 500 is provided with windows 510 at positions corresponding to the liquid bladder cavities 311, and the number of windows 510 is consistent with the number of liquid bladder cavities 311 in the liquid bladder layer 300. When it is necessary to discharge the liquid reagent in the liquid bladder cavity 311, the liquid bladder unit 310 can be squeezed through the windows 510 provided in the liquid bladder cover 500, thereby releasing the liquid reagent therein.

[0058] In practical applications, the liquid bladder unit 310 can be moved toward the side closer to the puncture structure 200 by mechanical means such as pressing down.

[0059] In some embodiments of this application, the liquid bladder 500 is provided with a first snap-fit ​​structure 520, and the substrate 100 is provided with a second snap-fit ​​structure 120, with the first snap-fit ​​structure 520 and the second snap-fit ​​structure 120 engaging and connecting. In this way, the liquid bladder 500 can be snapped and fixed onto the substrate 100.

[0060] In one possible implementation, the first snap-fit ​​structure 520 is specifically a snap-fit ​​groove, and the second snap-fit ​​structure 120 is specifically a snap-fit ​​protrusion, the snap-fit ​​protrusion matching the snap-fit ​​groove. By engaging the snap-fit ​​groove and the snap-fit ​​protrusion, the liquid bladder 500 can be snapped onto the substrate 100, thereby fixing the liquid bladder 500 to the substrate 100.

[0061] In some embodiments of this application, the liquid bladder 500 includes a limiting portion 530, which abuts against three sides of the substrate 100. Combined with the snapping action of the first snap-fit ​​structure 520 and the second snap-fit ​​structure 120, the liquid bladder 500 can be fixed to the substrate 100. Since the limiting portion 530 abuts against the three sides of the substrate 100, the probability of the liquid bladder 500 moving relative to the substrate 100 can be reduced, thus helping to protect the liquid bladder layer 300 from damage by the liquid bladder 500.

[0062] The second aspect of this application discloses a microfluidic detection chip, including the liquid sac structure as described above, which has all the technical effects of the aforementioned liquid sac structure, and will not be repeated here.

[0063] It should be noted that in practical applications, by processing cavities such as reagent cavities, buffer cavities, and reaction cavities at other locations on the substrate 100, and simultaneously processing necessary connecting channels, a microfluidic chip with the aforementioned liquid bladder structure can be obtained.

[0064] In the description of this application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0065] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0066] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0067] In the description of this specification, specific features, structures, materials, or characteristics may be combined in any suitable manner in one or more embodiments or examples.

[0068] It should be understood that although the steps in the flowcharts of the accompanying figures are shown sequentially as indicated by the arrows, these steps are not necessarily executed in the order indicated by the arrows. Unless explicitly stated herein, there is no strict order restriction on the execution of these steps, and they can be executed in other orders. Moreover, at least some steps in the flowcharts of the accompanying figures may include multiple sub-steps or multiple stages. These sub-steps or stages are not necessarily completed at the same time, but can be executed at different times, and their execution order is not necessarily sequential, but can be performed alternately or in turn with other steps or at least some of the sub-steps or stages of other steps.

Claims

1. A liquid bladder structure, characterized in that, include: The substrate has multiple spaced grooves; A piercing structure is provided in each of the grooves; A liquid bladder layer is mounted on one side of the substrate. The liquid bladder layer has a plurality of liquid bladder units, each of which has a liquid bladder cavity, and the liquid bladder cavity corresponds to at least one of the positions of the puncture structure. The puncturing structure is used to puncture the fluid sac cavity located on one side thereon, and there is a gap between the end of the puncturing structure and the fluid sac cavity.

2. The liquid bladder structure according to claim 1, characterized in that, The puncture structure includes a conical portion, and the substrate has a liquid outlet hole on one side corresponding to the conical portion.

3. The liquid bladder structure according to claim 2, characterized in that, The tapered portion has a recessed area, and the outline of the recessed area is tapered.

4. The liquid bladder structure according to any one of claims 1 to 3, characterized in that, The liquid bladder unit includes a liquid bladder shell and a liquid bladder sealing membrane. A liquid bladder cavity with an open end is formed on the liquid bladder shell, and the liquid bladder sealing membrane is sealed at the opening of the liquid bladder cavity to enclose the closed liquid bladder cavity.

5. The liquid bladder structure according to claim 1, characterized in that, It also includes an adhesive layer disposed between the liquid bladder layer and the substrate.

6. The liquid bladder structure according to claim 5, characterized in that, The adhesive layer has through holes corresponding to the positions of the liquid bladder cavity.

7. The liquid bladder structure according to claim 1, characterized in that, It also includes a liquid bladder cover, which is disposed on the outer periphery of the liquid bladder layer, wherein the liquid bladder cover has a window provided at the position corresponding to the liquid bladder cavity.

8. The liquid bladder structure according to claim 7, characterized in that, The liquid bladder is provided with a first snap-fit ​​structure, and the substrate is provided with a second snap-fit ​​structure, wherein the first snap-fit ​​structure and the second snap-fit ​​structure are connected in cooperation.

9. The liquid bladder structure according to claim 7 or 8, characterized in that, The liquid bladder includes a limiting portion that abuts against three sides of the substrate.

10. A microfluidic detection chip, characterized in that, Includes the liquid bladder structure as described in any one of claims 1 to 9.

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