Immunofluorescent staining tool kit

Abstract

The utility model belongs to the technical field of immunofluorescent staining, and particularly relates to an immunofluorescent staining tool kit which comprises a base, the cleaning module is detachably mounted on the base, and the cleaning module is used for cleaning the used cover glass; the dyeing module is detachably mounted on the cleaning module, and the dyeing module is used for dyeing the cover glass; and the cover plate is detachably mounted on the dyeing module. The utility model is convenient to use and can ensure the dyeing effect. The evaporation of the reagent in the long-time incubation process can be reduced, so that the dyeing effect is more stable. In addition, independent dyeing during simultaneous incubation and transfer of a plurality of cover glass can be guaranteed, and mutual confusion can be avoided. The cleaning module can simultaneously meet different requirements of rapid cleaning and long-time cleaning of the cover glass, reagent adding can be completed at a time, and the cumbersome step of replacing reagents for multiple times is omitted. Meanwhile, due to the detachable design, all the components can be cleaned and dried more easily, and the cover glass can be taken and placed conveniently, and cleaning liquid can be replaced conveniently.

Description

Technical Field

[0001] This utility model belongs to the field of immunofluorescence staining technology, and in particular relates to an immunofluorescence staining tool kit. Background Technology

[0002] Immunofluorescence staining is a technique based on the localization of cells by the reaction of fluorescently labeled antigens (antibodies) with corresponding molecules in tissues or cells. Currently, the commonly used immunofluorescence staining sample is a cell slide, typically a circular coverslip with a radius of 6 mm. The staining process requires steps such as washing, blocking, and antibody incubation. To save on antibodies and reagents, the most common method is to invert the coverslip onto a pre-placed droplet on a sealing film for washing, blocking, and antibody incubation. However, due to the smoothness of the sealing film, the coverslips can easily shift when the film is moved, causing multiple coverslips to overlap or slip off the film. Additionally, insufficient liquid during cleaning can lead to incomplete cleaning.

[0003] Therefore, it is necessary to design an immunofluorescence staining tool kit to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide an immunofluorescence staining tool kit to solve the above-mentioned problems.

[0005] To achieve the above objectives, this utility model provides the following solution: an immunofluorescence staining tool kit, comprising...

[0006] Base;

[0007] A cleaning module is detachably mounted on the base, and the cleaning module is used to clean the cover glass after use;

[0008] A staining module is detachably mounted on the cleaning module, and the staining module is used to stain coverslips;

[0009] A cover plate is detachably mounted on the dyeing module.

[0010] Based on the immunofluorescence staining tool kit of this utility model, the cleaning module includes a tray, which is detachably installed on the base. A cleaning seat is fixedly connected to the tray, and the cleaning seat has two receiving slots inside. The two receiving slots are arranged side by side with intervals. A first cleaning slot is detachably installed in one receiving slot, and a second cleaning slot is detachably installed in the other receiving slot.

[0011] Based on the immunofluorescence staining tool kit of this utility model, the cleaning tank is provided with a plurality of sub-slots, and the plurality of sub-slots are arranged in an array along the length and width directions of the cleaning tank.

[0012] The cleaning tank 2 has several sub-slots 2, which are arranged in an array along the length and width directions of the cleaning tank 2.

[0013] Based on the present invention, the immunofluorescence staining tool kit includes a staining plate one and a staining plate two. The staining plate one is detachably installed on the top of the washing seat, the staining plate two is detachably installed on the top of the staining plate one, and the cover plate is detachably installed on the top of the staining plate two.

[0014] Based on the present invention, an immunofluorescence staining tool kit is provided with a protrusion at the top of the staining plate, and a plurality of staining grooves are provided on the protrusion, and the plurality of staining grooves are arranged in an array.

[0015] The top of the dyeing plate is provided with a boss, and a plurality of shaping columns are provided on the boss, and the plurality of shaping columns are arranged in an array.

[0016] Based on the present invention, the immunofluorescence staining tool kit has a storage cavity at the top of the base, and an annular protrusion is provided at the top of the side wall of the storage cavity.

[0017] Based on the immunofluorescence staining tool kit of this utility model, the bottom end of the tray is provided with a groove, which is adapted to the annular protrusion.

[0018] Based on the present invention, an immunofluorescence staining tool kit has a groove at the bottom of the staining plate and two annular protrusions on the outer sides of the two receiving grooves, and the groove and the annular protrusions are adapted to each other.

[0019] Based on the immunofluorescence staining tool kit of this utility model, the bottom end of the staining plate two is provided with a groove three, which is adapted to the protrusion one.

[0020] Based on the present invention, the immunofluorescence staining tool kit has a groove four at the bottom end of the cover plate, which is adapted to the boss two.

[0021] Compared with the prior art, the present invention has the following advantages and technical effects:

[0022] 1. This invention is convenient to use and ensures good staining results. It reduces reagent evaporation during long incubation periods, resulting in more stable staining. Furthermore, it ensures independent staining during the simultaneous incubation and transfer of multiple coverslips, preventing confusion between them.

[0023] 2. The cleaning module of this invention can simultaneously meet the different needs of rapid cleaning and long-term cleaning of coverslips. It can complete the addition of reagents in one go, eliminating the tedious steps of changing reagents multiple times. At the same time, the detachable design makes it easier to clean and dry each component, and also facilitates the removal and placement of coverslips and the replacement of cleaning solution.

[0024] 3. This invention assists in the cleaning, blocking, and antibody incubation processes during immunofluorescence staining of cell slides. The overall design features a stackable, layered structure, facilitating storage and allowing for flexible addition of necessary components according to experimental needs. This improves the efficiency of cell immunofluorescence staining and makes the staining results more stable. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the overall design of this utility model;

[0027] Figure 2 For the purpose of this utility model explosion Figure One ;

[0028] Figure 3 For the purpose of this utility model explosion Figure Two .

[0029] The components are as follows: 1. Base; 101. Storage cavity; 102. Ring protrusion one; 2. Support plate; 201. Groove one; 3. Cleaning seat; 301. Receiving groove; 302. Ring protrusion two; 4. Dyeing plate one; 401. Boss one; 402. Dyeing tank; 403. Groove two; 5. Dyeing plate two; 501. Boss two; 502. Shaping column; 503. Groove three; 6. Cover plate; 601. Groove four; 7. Cleaning tank one; 701. Dividing groove one; 8. Cleaning tank two; 801. Dividing groove two. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] Reference Figures 1 to 3 As shown, this utility model provides an immunofluorescence staining tool kit, including...

[0033] Base 1;

[0034] The cleaning module is detachably mounted on the base 1 and is used to clean the cover glass after use.

[0035] The staining module is detachably mounted on the cleaning module and is used to stain the coverslips.

[0036] Cover plate 6 is detachably installed on the dyeing module.

[0037] Furthermore, the cleaning module includes a tray 2, which is detachably mounted on the base 1. A cleaning seat 3 is fixedly connected to the tray 2. The cleaning seat 3 has two receiving slots 301 inside, which are arranged side by side with intervals. A first cleaning slot 7 is detachably installed in one receiving slot 301, and a second cleaning slot 8 is detachably installed in the other receiving slot 301.

[0038] Furthermore, the cleaning tank 7 is provided with a plurality of sub-slots 701, which are arranged in an array along the length and width of the cleaning tank 7.

[0039] The cleaning tank 2 8 has several sub-slots 2 801, which are arranged in an array along the length and width of the cleaning tank 2 8.

[0040] Furthermore, the dyeing module includes dyeing plate 4 and dyeing plate 5. Dyeing plate 4 is detachably installed on the top of the cleaning seat 3, dyeing plate 5 is detachably installed on the top of dyeing plate 4, and cover plate 6 is detachably installed on the top of dyeing plate 5.

[0041] Furthermore, a boss 401 is provided at the top of the dyeing plate 4, and a plurality of dyeing grooves 402 are provided on the boss 401, and the plurality of dyeing grooves 402 are arranged in an array.

[0042] The top of the dyeing plate 25 is provided with a boss 2501, and a number of shaping columns 502 are provided on the boss 2501, and the number of shaping columns 502 are arranged in an array.

[0043] Furthermore, a storage cavity 101 is provided at the top of the base 1, and an annular protrusion 102 is provided at the top of the side wall of the storage cavity 101.

[0044] Furthermore, a groove 201 is provided at the bottom end of the tray 2, which is adapted to the annular protrusion 102.

[0045] Furthermore, a groove 403 is provided at the bottom of the dyeing plate 4, and an annular protrusion 302 is provided on the outer side of the two receiving grooves 301, with the groove 403 and the annular protrusion 302 being compatible.

[0046] Furthermore, the bottom end of the dyeing plate 25 is provided with a groove 3 503, which is compatible with the boss 1 401.

[0047] Furthermore, a groove 601 is provided at the bottom of the cover plate 6, which is adapted to the boss 501.

[0048] The base 1, cleaning seat 3, staining plate one 4, staining plate two 5, and cover plate 6 can be stacked sequentially. Each component has appropriately sized protrusions or grooves on its upper and lower edges to facilitate connection between components. Staining plate one 4 can be added as needed; if more staining tanks are required for the experiment, additional staining plate one 4 can be installed. All components are made of opaque, high-temperature, high-pressure steam-sterilizable polypropylene (PP) material to meet the requirements for light protection and sterility during the staining process. Cover plate 6 can be placed on the cleaning seat 3, staining plate one 4, and staining plate two 5 to meet the light protection requirements during cleaning and incubation stages.

[0049] The first staining plate 4 has nine staining grooves 402, each with a radius of 12.5 mm and a depth of 3 mm. These grooves can independently accommodate coverslips and prevent the sealing film from stretching excessively when extruding into the grooves, ensuring that the placement and removal of coverslips are not affected by edge height. The second staining plate 5 has shaping pillars 502 corresponding to the staining grooves 402. These pillars have rounded edges and a diameter slightly smaller than the staining grooves 402 to prevent the sealing film from being cut when extruding it. Both the first staining plate 4 and the second staining plate 5 have grooves at their bottoms for connection and fixation with adjacent components.

[0050] The cleaning tank 3 has a receiving tank 301 for long-term cleaning of coverslips and for storing them in a light-proof environment. Cleaning tank 7 includes multiple sub-slots 701, each 17mm x 6mm in length and width and 10mm in height. Coverslips should be placed diagonally into sub-slots 701 with the cell side facing upwards during cleaning. The diagonal lines of sub-slots 701 are slightly smaller than the diameter of the coverslips, facilitating easy placement and removal while ensuring that cells above sub-slots are still immersed due to surface tension. Cleaning tank 8 has multiple sub-slots 801, each 17mm x 10mm in length and width and 20mm in height. Sufficient cleaning solution can be added for rapid cleaning of coverslips, while ensuring that if a coverslip is accidentally dropped into a sub-slot 801, it will not be difficult to remove as it will be completely stuck to the bottom. Cleaning tanks 7 and 8 are detachable components; they can be placed in the receiving tank 301 for convenient storage and light protection, or removed for easy placement and removal of coverslips and for cleaning and drying the coverslips themselves.

[0051] The base 1 is designed to accommodate multiple sealing films that can just cover the dyeing plate 4, and can also be connected to the bottom of the cleaning seat 3 for storage.

[0052] In the description of this utility model, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0053] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Various modifications and improvements to the technical solutions of the present utility model made by those skilled in the art without departing from the spirit of the present utility model should fall within the protection scope of the present utility model.

Claims

1. An immunofluorescence staining tool kit, characterized in that, include Base (1); A cleaning module is detachably installed on the base (1) and is used to clean the cover glass after use. A staining module is detachably mounted on the cleaning module, and the staining module is used to stain coverslips; The cover plate (6) is detachably installed on the dyeing module.

2. The immunofluorescence staining kit according to claim 1, characterized in that, The cleaning module includes a tray (2), which is detachably mounted on the base (1). A cleaning seat (3) is fixedly connected to the tray (2). The cleaning seat (3) has two receiving slots (301) inside. The two receiving slots (301) are arranged side by side with intervals. A first cleaning slot (7) is detachably installed in one receiving slot (301), and a second cleaning slot (8) is detachably installed in the other receiving slot (301).

3. The immunofluorescence staining tool kit according to claim 2, characterized in that, The cleaning tank (7) is provided with a plurality of sub-slots (701), and the plurality of sub-slots (701) are arranged in an array along the length and width directions of the cleaning tank (7); The cleaning tank 2 (8) is provided with a plurality of sub-slots 2 (801), and the plurality of sub-slots 2 (801) are arranged in an array along the length and width directions of the cleaning tank 2 (8).

4. The immunofluorescence staining kit according to claim 2, characterized in that, The dyeing module includes a dyeing plate one (4) and a dyeing plate two (5). The dyeing plate one (4) is detachably installed on the top of the cleaning seat (3). The dyeing plate two (5) is detachably installed on the top of the dyeing plate one (4). The cover plate (6) is detachably installed on the top of the dyeing plate two (5).

5. The immunofluorescence staining kit according to claim 4, characterized in that, The top of the dyeing plate (4) is provided with a boss (401), and a plurality of dyeing grooves (402) are provided on the boss (401), and the plurality of dyeing grooves (402) are arranged in an array. The top of the dyeing plate 2 (5) is provided with a boss 2 (501), and a plurality of shaping columns (502) are provided on the boss 2 (501), and the plurality of shaping columns (502) are arranged in an array.

6. The immunofluorescence staining kit according to claim 5, characterized in that, The base (1) has a storage cavity (101) at its top, and the storage cavity (101) has an annular protrusion (102) at its top side wall.

7. The immunofluorescence staining kit according to claim 6, characterized in that, The bottom end of the tray (2) is provided with a groove (201), which is adapted to the annular protrusion (102).

8. The immunofluorescence staining kit according to claim 7, characterized in that, The bottom end of the dyeing plate (4) is provided with a groove (403), and the outer sides of the two receiving grooves (301) are provided with annular protrusions (302), and the groove (403) and the annular protrusions (302) are adapted to each other.

9. The immunofluorescence staining kit according to claim 8, characterized in that, The bottom end of the second dyeing plate (5) is provided with a groove three (503), which is adapted to the first boss (401).

10. The immunofluorescence staining tool kit according to claim 9, characterized in that, The bottom end of the cover plate (6) is provided with a groove four (601), which is adapted to the boss two (501).

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