Electrochemiluminescence-electrophoresis incubation system

Abstract

The utility model relates to the technical field of biosensing, and discloses an electrogenerated chemiluminescence-electrophoresis incubation system which comprises an electrogenerated chemiluminescence-electrophoresis analysis module, a first container, a first cover body, a second container, a second cover body, a working electrode, a platinum disc, a conduit, a valve, a reference electrode and a counter electrode, a valve is arranged on the conduit, the platinum plate is arranged at the bottom of the first container, the first cover body is detachably arranged at the top of the first container, the working electrode is mounted on the first cover body, the reference electrode and the counter electrode are both mounted on the second cover body, and the second cover body is detachably arranged at the top of the second container; the platinum plate, the working electrode, the reference electrode and the counter electrode are all connected with the electrochemiluminescence-electrophoresis analysis module. According to the utility model, the experimental process can be obviously simplified, the detection speed is accelerated, the detection time is greatly shortened, and errors of experimental detection results caused by interference of factors such as environment and the like on the working electrode in the transfer process are avoided.

Description

Technical Field

[0001] This utility model relates to the field of biosensing technology, and in particular to an electrochemiluminescence-electrophoresis incubation system. Background Technology

[0002] In the prior art, Chinese Patent Publication No. CN117129549A discloses a system, sensor, and method for detecting CEA based on electrophoresis and ECL principles, which involves an electrophoresis apparatus. The electrophoresis apparatus is an electrolytic cup with a platinum wire disc at the bottom, which is connected to the negative terminal of the power supply of the electrophoresis apparatus; the working electrode of the electrochemiluminescence immunoassay sensor is connected to the positive terminal of the power supply; the analyte is driven to the electrode surface by an upward electric field for enrichment. However, in this technology, the electrophoresis apparatus and the ECL detection device are two independent devices. Therefore, in the existing technology, the working electrode needs to be switched back and forth between the two different devices during electrophoresis incubation and detection. This not only makes the experimental process more cumbersome, but also may contaminate the electrode during the transfer process, thereby increasing the possibility of non-specific binding on the electrode surface, greatly increasing the experimental complexity. Utility Model Content

[0003] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide an electrochemiluminescence-electrophoresis incubation system, which for the first time integrates an electrophoresis device with an ECL detection device. One part is the electrophoresis zone, and the other part is the reference / platinum wire electrode, separated by a controllable glass valve, achieving integrated electrophoresis and detection, greatly simplifying the experimental procedure. Furthermore, based on the principle of electrochemiluminescence generating light through voltage, this invention innovatively combines an electrophoresis instrument with an electrochemiluminescence instrument, providing new ideas and directions for commercial ECL detection.

[0004] The present invention adopts the following technical solution:

[0005] An electrochemiluminescence-electrophoresis incubation system includes an electrochemiluminescence-electrophoresis analysis module, a first container, a first cover, a second container, a second cover, a working electrode, a platinum disc, a conduit, a valve, a reference electrode, and a counter electrode. The first container is connected to the second container via the conduit, and the valve is installed on the conduit. The platinum disc is located at the bottom of the first container. The first cover is detachably located at the top of the first container. The working electrode is mounted on the first cover. When the first cover is located at the top of the first container, a portion of the working electrode extends into the first container. The reference electrode and the counter electrode are both mounted on the second cover. The second cover is detachably located at the top of the second container. When the second cover is located at the top of the second container, portions of both the reference electrode and the counter electrode extend into the second container. The platinum disc, working electrode, reference electrode, and counter electrode are all connected to the electrochemiluminescence-electrophoresis analysis module.

[0006] Preferably, the above-mentioned electrochemiluminescence-electrophoresis incubation system further includes a chemiluminescence detection dark chamber, in which the first container and the second container are both disposed, the chemiluminescence detection dark chamber is provided with a wiring hole, the electrochemiluminescence-electrophoresis analysis module is disposed on the outside of the chemiluminescence detection dark chamber, and the platinum disk, working electrode, reference electrode and counter electrode are all sealed and connected to the electrochemiluminescence-electrophoresis analysis module through the wiring hole via a wire.

[0007] Preferably, in the above-mentioned electrochemiluminescence-electrophoresis incubation system, the first cover is provided with liquid holes.

[0008] Preferably, the above-mentioned electrochemiluminescence-electrophoresis incubation system further includes a support assembly, which is disposed inside the chemiluminescence detection dark chamber. The support assembly includes a support column and a crossbar, the crossbar being fixedly connected to the support column and perpendicular to the support column. The crossbar is provided with a first through hole and a second through hole, and the first container and the second container are respectively disposed in the first through hole and the second through hole.

[0009] Preferably, in the above-mentioned electrochemiluminescence-electrophoresis incubation system, the electrochemiluminescence-electrophoresis analysis module includes a control module and a power supply module. The platinum disk and the counter electrode are both connected to the negative electrode of the power supply module via a wire. The working electrode and the reference electrode are both connected to the positive electrode of the power supply module via a wire. An ammeter is installed in the connection circuit between the working electrode and the positive electrode of the power supply module. A voltmeter is installed in the connection circuit between the reference electrode and the positive electrode of the power supply module. A first switch, a second switch, a third switch, and a fourth switch are respectively installed on the wires connecting the platinum disk, the working electrode, the reference electrode, and the counter electrode to the power supply module. The signal output terminals of the ammeter and the voltmeter are connected to the signal input terminals of the control module. The signal output terminal of the control module is connected to the signal input terminals of the power supply module, the first switch, the second switch, the third switch, and the fourth switch.

[0010] Preferably, in the above-mentioned electrochemiluminescence-electrophoresis incubation system, the working electrode is a magnetic glassy carbon electrode.

[0011] Preferably, in the above-mentioned electrochemiluminescence-electrophoresis incubation system, the reference electrode is an Ag / AgCl electrode.

[0012] Preferably, in the above-mentioned electrochemiluminescence-electrophoresis incubation system, the counter electrode is a platinum wire electrode.

[0013] Preferably, in the above-mentioned electrochemiluminescence-electrophoresis incubation system, the leads of the platinum disk, working electrode, reference electrode, and counter electrode are respectively connected to one end of a wire via an electrode clamp, and the other end of the wire is connected to the electrochemiluminescence-electrophoresis analysis module.

[0014] Preferably, in the above-mentioned electrochemiluminescence-electrophoresis incubation system, the conduit is a glass conduit and the valve is a glass valve.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] This invention integrates the detection device of an electrochemiluminescence sensor with an electrophoresis device, significantly simplifying the experimental procedure, accelerating the detection speed, and greatly shortening the detection time. Simultaneously, it avoids errors in experimental results caused by environmental factors during the transfer of the working electrode. The controllable valve design ensures that the electrophoresis effect is unaffected, thus achieving the integration of the two devices without mutual interference, improving both detection speed and sensitivity. Furthermore, the integrated device design provides new ideas and directions for commercial ECL detection. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of an electrochemiluminescence-electrophoresis incubation system according to an embodiment of the present invention;

[0019] Figure 2 This is a structural diagram of an electrochemiluminescence-electrophoresis incubation system according to an embodiment of the present invention, when a chemiluminescence detection dark box is provided;

[0020] Figure 3 This is a schematic diagram of the structure of a support component in an electrochemiluminescence-electrophoresis incubation system according to an embodiment of the present invention;

[0021] Figure 4 This is a schematic diagram showing the connection between the electrochemiluminescence-electrophoresis analysis module and each electrode in an electrochemiluminescence-electrophoresis incubation system according to an embodiment of the present invention.

[0022] Figure label:

[0023] 1. Electrochemiluminescence-electrophoresis analysis module; 101. Control module; 102. Power supply module; 103. Ammeter; 104. Voltmeter; 105. First switch; 106. Second switch; 107. Third switch; 108. Fourth switch; 2. First container; 3. First cover; 4. Second container; 5. Second cover; 6. Working electrode; 7. Platinum disk; 8. Conduit; 9. Valve; 10. Reference electrode; 11. Counter electrode; 12. Chemiluminescence detection dark box; 13. Wiring hole; 14. Liquid hole; 15. Support assembly; 1501. Support column; 1502. Crossbar; 1503. First through hole; 1504. Second through hole; 16. Electrode clamp; 17. Wire. Detailed Implementation

[0024] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model. It should be noted that, unless otherwise specified, the following embodiments and features described therein can be combined with each other.

[0025] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0026] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0027] The present invention will now be further described with reference to the accompanying drawings.

[0028] This utility model provides an electrochemiluminescence-electrophoresis incubation system, such as Figure 1 As shown, the electrochemiluminescence-electrophoresis incubation system includes an electrochemiluminescence-electrophoresis analysis module 1, a first container 2, a first cover 3, a second container 4, a second cover 5, a working electrode 6, a platinum disc 7, a conduit 8, a valve 9, a reference electrode 10, and a counter electrode 11. The first container 2 is connected to the second container 2 via the conduit 8, and the valve 9 is installed on the conduit 8. The platinum disc 7 is located at the bottom of the first container 2, and the first cover 3 is detachably installed at the top of the first container 2. The working electrode 6 is mounted on the first cover 3. When the first cover 3 is placed on top of the first container 2, part of the working electrode 6 extends into the first container 4. The reference electrode 10 and the counter electrode 11 are both mounted on the second cover 3. The second cover 3 is detachably placed on top of the second container 4. When the second cover 5 is placed on top of the second container 4, parts of the reference electrode 10 and the counter electrode 11 extend into the second container 4. The platinum disk 7, the working electrode 6, the reference electrode 10, and the counter electrode 11 are all connected to the electrochemiluminescence-electrophoresis analysis module 1.

[0029] In practice, valve 9 is first closed to disconnect the first container 2 and the second container 4. Then, a sample solution is added to the first container 2. A DC voltage is applied between the platinum disk 7 and the working electrode 6 via the electrochemiluminescence-electrophoresis analysis module 1 to create an electric field for electrophoresis incubation. Charged molecules in the sample migrate towards the opposite electrode under the influence of the electric field, achieving separation based on molecular weight or charge differences. During electrophoresis incubation, valve 9 on the conduit 8 is closed to prevent liquid in the first container 2 from flowing into the second container 4, maintaining the stability of the electrophoresis process. After electrophoresis incubation, the electrochemiluminescence-electrophoresis analysis module 1 stops applying the DC voltage and opens valve 9. The solution obtained from electrophoresis incubation then enters the second container 2 through conduit 8. Based on the principle of a U-shaped connecting tube, the solution is now located between the first container 2 and the second container 4. An ELC probe can be added to the second container 2 before opening valve 9, or an ELC probe can be pre-placed in the second container 4 to better perform ELC detection on the solution after opening valve 9. After opening valve 9, the three-electrode system is activated by the electrochemiluminescence-electrophoresis analysis module 1, that is, the working electrode 6, the reference electrode 10 and the counter electrode 11 form two circuits, and the detection of ELC signal can be realized through the three-electrode system.

[0030] In this embodiment, the electrochemiluminescence-electrophoresis analysis module 1 can be selected from an existing electrochemical station, which provides a power source and acquires the corresponding detection signal.

[0031] In some embodiments, such as Figure 2 As shown, the electrochemiluminescence-electrophoresis incubation system also includes a chemiluminescence detection dark box 12. The first container 2 and the second container 4 are both disposed inside the chemiluminescence detection dark box 12. The chemiluminescence detection dark box 12 is provided with a wiring hole 13. The electrochemiluminescence-electrophoresis analysis module 1 is disposed outside the chemiluminescence detection dark box 12. The platinum disk 7, the working electrode 6, the reference electrode 10 and the counter electrode 11 are all sealed and connected to the electrochemiluminescence-electrophoresis analysis module 1 through the wiring hole 13 via a wire 17.

[0032] In this embodiment, the core function of the chemiluminescence detection dark chamber 12 is to eliminate the interference of ambient light on the electrochemiluminescence (ECL) signal. ECL signals are typically weak, and ambient light significantly increases background noise, reducing detection sensitivity. The platinum disk 7, working electrode 6, reference electrode 10, and counter electrode 11 are all sealed through a wire 17 via the wiring hole 13 and connected to the electrochemiluminescence-electrophoresis analysis module 1 to ensure the light-shielding properties of the chemiluminescence detection dark chamber 12.

[0033] It should be noted that, based on the above description of the workflow of the electrochemiluminescence-electrophoresis incubation system, some experimental materials need to be added when using this system. Therefore, the chemiluminescence detection dark box 12 is a box with opening and closing functions; that is, the chemiluminescence detection dark box 12 includes a box body and a door. For example, the box body is... Figure 2 When the container is rectangular in shape as shown, a door that can be opened and closed is provided on one side of the container. To add experimental items, the door is opened, and after addition, the door is closed, ensuring that the first container 2 and the second container 4 are in a highly light-blocking environment. For ease of operation, the door can be located on the upper surface of the container.

[0034] In some embodiments, such as Figure 1 As shown, the first cover 3 is provided with a liquid hole 14. The liquid hole 14 is used to facilitate the addition of the corresponding sample to the first cover 3. Of course, in the design without the liquid hole 14, the first cover 3 can be removed from the top of the first container 2 before adding the corresponding sample.

[0035] In some embodiments, such as Figure 3 As shown, the electrochemiluminescence-electrophoresis incubation system also includes a support assembly 15, which is disposed inside the chemiluminescence detection dark box 12. The support assembly 15 includes a support column 1501 and a crossbar 1502. The crossbar 1502 is fixedly connected to the support column 1501 and is perpendicular to the support column 1501. The crossbar 1502 is provided with a first through hole 1503 and a second through hole 1504. The first container 2 and the second container 3 are respectively disposed in the first through hole 1503 and the second through hole 1504.

[0036] The support component 15, through its mechanical fixing and spatial separation design, solves the stability problem of the container during electrophoresis incubation and ECL detection, while optimizing the controllability of experimental conditions within the dark chamber. The collaborative work of this support component 15 with the dark chamber and electrode module further enhances the reliability of the system in micro-analysis and high-sensitivity detection scenarios.

[0037] Specifically, the support assembly 15, through the rigid structure of the support column 1501 and the crossbar 1502, provides physical fixation for the first container 2 and the second container 4, ensuring their stable spatial position within the chemiluminescence detection dark chamber 12 and avoiding experimental errors caused by container shaking or tilting. The first through hole 1503 and the second through hole 1504 on the crossbar 1502 are nested and fixed to the first container 2 and the second container 4 respectively, achieving positioning through the tight fit between the hole wall and the container (or by adding clamps, such as spring support plates on the hole wall, using spring force to fix the container against the hole wall). The support column 1501 is fixed to the inner wall or bottom of the dark chamber 12, forming a vertical support frame to ensure the horizontal stability of the crossbar 1502. The first container 2 (electrophoresis incubation) and the second container 4 (ECL detection) are placed at different positions on the crossbar 1502 through the through holes, achieving physical spatial separation and avoiding cross-interference (such as interference from the electrophoresis electric field on ECL detection). The two containers are connected by conduit 8. The support assembly ensures that the connection path of conduit 8 is straight and without bends, guaranteeing smooth flow of solution after valve 9 is opened. After the support assembly 15 fixes the position of the containers, it can ensure the precise relative position of the electrodes (working electrode 6, reference electrode 10, counter electrode 11) and the solution in the containers, maintaining the stability of the electrochemical reaction.

[0038] During the electrophoresis incubation stage, a DC voltage is applied between the platinum disk 7 and the working electrode 6. Shaking of the container can lead to uneven electric field distribution, affecting the migration path of charged molecules. The support assembly 15 eliminates this risk through rigid fixation. During the ECL detection stage, the potential control of the three-electrode system is sensitive to electrode position; fixing the container avoids signal fluctuations caused by poor electrode-solution contact. The fixed design of the support assembly 15 standardizes the installation positions of the first container 2 and the second container 4, ensuring consistent conditions between different experimental batches and improving data comparability.

[0039] Furthermore, the support component 15, combined with the chemiluminescence detection dark chamber 12, provides a dark environment and ensures the physical stability of the experimental process by fixing the container. When the wire 17 of the wiring hole 13 passes through the chemiluminescence detection dark chamber 12, the support component 15 can help fix the direction of the wire, preventing the wire from swinging and interfering with the position of the container or the flow of the solution.

[0040] In some embodiments, such as Figure 4As shown, the electrochemiluminescence-electrophoresis analysis module 1 includes a control module 101 and a power supply module 102. The platinum disk 7 and the counter electrode 11 are both connected to the negative electrode of the power supply module 102 through a wire 17. The working electrode 6 and the reference electrode 10 are both connected to the positive electrode of the power supply module 102 through a wire 17. An ammeter 103 is installed on the connection circuit between the working electrode 6 and the positive electrode of the power supply module 102. A voltmeter 104 is installed on the connection circuit between the reference electrode 10 and the positive electrode of the power supply module 102. A first switch 105, a second switch 106, a third switch 107, and a fourth switch 108 are respectively installed on the wires connecting the platinum disk 7, the working electrode 6, the reference electrode 10, and the counter electrode 11 to the power supply module. The signal output terminals of the ammeter 103 and the voltmeter 104 are connected to the signal input terminals of the control module 101. The signal output terminals of the control module 101 are connected to the signal input terminals of the power supply module 102, the first switch 105, the second switch 106, the third switch 107, and the fourth switch 108.

[0041] In this embodiment, the platinum disk 7 is connected to the negative electrode of the power module 102, forming the negative electrode of the electric field during the electrophoresis incubation stage. The working electrode 6 is connected to the positive electrode of the power module 102, forming the positive electrode of the electric field during the electrophoresis incubation stage, thus forming the circuit loop for the electrophoresis incubation stage. The counter electrode 11 is connected to the negative electrode of the power module 102, and the working electrode 6 and the reference electrode 10 are connected to the positive electrode of the power module 102, constituting a three-electrode system for ECL detection. During the electrophoresis stage, a DC electric field is formed between the platinum disk 7 (negative electrode) and the working electrode 6 (positive electrode), driving the migration and separation of charged molecules. During the ECL detection stage, the working electrode 6 (positive electrode), the reference electrode 10 (potential reference), and the counter electrode 11 (negative electrode) constitute a three-electrode system, precisely controlling the potential of the working electrode to excite chemiluminescence.

[0042] Each electrode circuit (platinum disk 7, working electrode 6, reference electrode 10, counter electrode 11) is equipped with an independent switch (first switch 105 to fourth switch 108), which is remotely controlled by the control module 101 to achieve phased circuit switching. Specifically, the control module 101 can control the power module 102, the first switch 105, the second switch 106, the third switch 107, and the fourth switch 108 in the following ways:

[0043] During the electrophoresis stage, the first switch 105 (negative electrode of platinum disk) and the second switch 106 (positive electrode of working electrode) are closed; the third switch 107 (reference electrode) and the fourth switch 108 (counter electrode) are opened, and the power module 102 is controlled to output a constant DC voltage to form a stable electric field. The stable electric field acts only between the platinum disk 7 and the working electrode 6, driving electrophoretic separation.

[0044] During the ECL detection stage, the first switch 105 and the second switch 106 are disconnected to stop the electrophoresis electric field; the third switch 107 (reference electrode) and the fourth switch 108 (counter electrode) are closed, and the power supply module 102 is controlled to switch to constant potential mode or apply pulse voltage to excite the chemiluminescence reaction. At this time, the three-electrode system (working electrode, reference electrode, counter electrode) is activated, and the potential is adjusted by the control module to excite the ECL reaction.

[0045] Ammeter 103 is connected in series in the positive electrode circuit of the working electrode 6 to monitor the total current during the electrophoresis stage or the reaction current during the ECL detection stage in real time, and feeds back the current signal to the control module 101. Voltmeter 104 is connected in parallel in the reference electrode circuit (positive terminal) to measure the potential difference between the working electrode 6 and the reference electrode 10, and feeds back the voltage signal to the control module 101. The reaction current during the ECL detection stage collected by ammeter 103 is the ELC signal detected in that stage. If the real-time data of ammeter 103 and voltmeter 104 exceed a preset threshold, the control module 101 immediately cuts off the corresponding switch (such as the first switch 105 or the fourth switch 108) to prevent electrode damage or solution overheating.

[0046] In some embodiments, the working electrode 6 is a magnetic glassy carbon electrode, the reference electrode 10 is an Ag / AgCl electrode, and the counter electrode 11 is a platinum wire electrode. It is understood that the specific model limitations of the working electrode 6, the reference electrode 10, and the counter electrode 11 described above are merely examples and do not constitute a limitation of this invention. Those skilled in the art can replace the models or materials of the electrodes according to actual detection requirements.

[0047] In some embodiments, such as Figure 1 As shown, the leads of platinum disk 7, working electrode 6, reference electrode 10 and counter electrode 11 are respectively connected to one end of wire 17 through electrode clip 16, and the other end of wire is connected to electrochemiluminescence-electrophoresis analysis module 1.

[0048] In this embodiment, the electrode clip 16 serves as a mechanical connection component, physically fixing the lead ends of the platinum disk 7, working electrode 6, reference electrode 10, and counter electrode 11 to one end of the wire 17, ensuring reliable contact between each electrode and the wire.

[0049] In some embodiments, conduit 8 is a glass conduit and valve 9 is a glass valve.

[0050] In this embodiment, the advantage of choosing glass materials for the conduit 8 and valve 9 is that glass (such as borosilicate glass) has high chemical inertness and does not react with most acids, alkalis, organic solvents, and electrochemically active substances (such as H2O2, luminol, and other ECL reagents). During the electrophoresis stage, this avoids impurities leached from the material of the conduit 8 or valve 9 from interfering with sample separation (such as metal ion catalytic side reactions); during the ECL detection stage, it prevents the material's own redox reactions from interfering with the luminescence signal, ensuring detection specificity.

[0051] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.

Claims

1. An electrochemiluminescence-electrophoresis incubation system, characterized in that, The system includes an electrochemiluminescence-electrophoresis analysis module, a first container, a first cover, a second container, a second cover, a working electrode, a platinum disc, a conduit, a valve, a reference electrode, and a counter electrode. The first container is connected to the second container via the conduit, and the valve is mounted on the conduit. The platinum disc is located at the bottom of the first container. The first cover is detachably mounted on the top of the first container. The working electrode is mounted on the first cover, and when the first cover is located on the top of the first container, a portion of the working electrode extends into the first container. The reference electrode and the counter electrode are both mounted on the second cover, which is detachably mounted on the top of the second container. When the second cover is located on the top of the second container, portions of both the reference electrode and the counter electrode extend into the first container. The platinum disc, working electrode, reference electrode, and counter electrode are all connected to the electrochemiluminescence-electrophoresis analysis module.

2. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, It also includes a chemiluminescence detection dark chamber, in which the first container and the second container are both disposed. The chemiluminescence detection dark chamber is provided with a wiring hole. The electrochemiluminescence-electrophoresis analysis module is disposed on the outside of the chemiluminescence detection dark chamber. The platinum disk, working electrode, reference electrode and counter electrode are all sealed and connected to the electrochemiluminescence-electrophoresis analysis module through the wiring hole via a wire.

3. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, The first cover is provided with liquid holes.

4. The electrochemiluminescence-electrophoresis incubation system according to claim 2, characterized in that, It also includes a support assembly, which is disposed inside the chemiluminescence detection dark chamber. The support assembly includes a support column and a crossbar. The crossbar is fixedly connected to the support column and is perpendicular to the support column. The crossbar is provided with a first through hole and a second through hole. The first container and the second container are respectively disposed in the first through hole and the second through hole.

5. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, The electrochemiluminescence-electrophoresis analysis module includes a control module and a power supply module. The platinum disk and the counter electrode are both connected to the negative electrode of the power supply module via a wire. The working electrode and the reference electrode are both connected to the positive electrode of the power supply module via a wire. An ammeter is installed in the connection circuit between the working electrode and the positive electrode of the power supply module, and a voltmeter is installed in the connection circuit between the reference electrode and the positive electrode of the power supply module. A first switch, a second switch, a third switch, and a fourth switch are respectively installed on the wires connecting the platinum disk, the working electrode, the reference electrode, and the counter electrode to the power supply module. The signal output terminals of the ammeter and the voltmeter are connected to the signal input terminals of the control module, and the signal output terminal of the control module is connected to the signal input terminals of the power supply module, the first switch, the second switch, the third switch, and the fourth switch.

6. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, The working electrode is a magnetic glassy carbon electrode.

7. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, The reference electrode is an Ag / AgCl electrode.

8. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, The counter electrode is a platinum wire electrode.

9. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, The leads of the platinum disk, working electrode, reference electrode, and counter electrode are connected to one end of a wire via electrode clamps, and the other end of the wire is connected to the electrochemiluminescence-electrophoresis analysis module.

10. The electrochemiluminescence-electrophoresis incubation system according to claim 1, characterized in that, The conduit is a glass conduit, and the valve is a glass valve.

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