Echinococcus egg collecting device
By employing a double-layer gradient screen structure and a closed filtration system, the problems of poor separation efficiency and contamination risk of Echinococcus granulosus eggs have been solved, achieving efficient and safe collection of Echinococcus granulosus eggs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- QINGHAI UNIVERSITY
- Filing Date
- 2025-07-04
- Publication Date
- 2026-05-19
AI Technical Summary
Existing technologies have poor separation efficiency for Echinococcus tapeworm eggs, and they are easily contaminated by the external environment during the separation process, making it difficult to achieve efficient and safe detection.
It adopts a double-layer gradient screen structure, including 150-mesh and 250-mesh metal screens, combined with modular connecting rods and sealing cap design to form a closed filtration system for efficient filtration and targeted enrichment of fecal samples.
This method achieves efficient filtration and enrichment of Echinococcus granulosus eggs in fecal samples, reducing interference from impurities, ensuring the cleanliness of the experimental environment and operational safety, and avoiding aerosol contamination.
Smart Images
Figure CN224250486U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of insect egg collection devices, and in particular to a device for collecting Echinococcus tapeworm eggs. Background Technology
[0002] Echinococcosis, also known as hydatid disease, is a parasitic disease caused by infection with the larvae (echinococcosis larvae) of the tapeworm Echinococcus. Common Echinococcus tapeworms in my country include *Echinococcos granulosus* (Eg) and *Echinococcus multilocularis* (Em), which cause cystic echinococcosis (CE) and alveolar echinococcosis (AE), respectively. As a zoonotic disease, it seriously endangers human health and life, causing immense suffering and a heavy economic burden on patients and their families, and also resulting in significant losses to livestock production.
[0003] The key to controlling echinococcosis lies in effectively controlling the source of infection and cutting off the transmission chain. The disease is caused by larvae, and the pathogen is the egg of the parasite. The primary source is dogs, which play a crucial role in infecting humans and livestock. Controlling the number of dogs as the source of infection and ensuring that dogs are free of worms and do not lay eggs are the core of echinococcosis control. As long as there are no Echinococcus suis eggs, there is no echinococcosis; that is, "no eggs, no disease." Therefore, accurately detecting Echinococcus suis egg contamination in the environment and diagnosing canine infection are essential for understanding the epidemiology of echinococcosis and implementing effective prevention and control measures.
[0004] The commonly used sample for detecting Echinococcus granulosus eggs is feces. Feces contain many impurities, making it difficult to isolate Echinococcus granulosus eggs. Moreover, Echinococcus granulosus eggs can be spread by dust, wind, water, etc., and the eggs are highly resistant, difficult to kill, and easily infected by the external environment.
[0005] Based on the above, the poor separation effect of Echinococcus granulosus eggs from feces and the susceptibility of Echinococcus granulosus eggs to external environmental contamination during the separation process are problems that urgently need to be solved by those skilled in the art. Utility Model Content
[0006] In view of this, the present invention provides a device for collecting Echinococcus tapeworm eggs.
[0007] To achieve the above-mentioned objectives, this utility model provides the following technical solution:
[0008] A device for collecting Echinococcus tapeworm eggs includes a sealing cap (1), a connecting rod (2), a 150-mesh sieve (3), a 250-mesh sieve (4), and a 50 mL centrifuge tube (5);
[0009] The 150-mesh sieve (3) and the 250-mesh sieve (4) are respectively connected to the connecting rod (2). The 150-mesh sieve (3) is close to the upper end of the 50mL centrifuge tube (5), and the 250-mesh sieve (4) is close to the lower end of the 50mL centrifuge tube (5) and is flush with the bottom of the connecting rod (2).
[0010] The spacing between the 150-mesh sieve (3) and the 250-mesh sieve (4) is 15-25 mm;
[0011] The length of the connecting rod (2) shall not exceed the length of the 50mL centrifuge tube (5);
[0012] Both the 150-mesh sieve (3) and the 250-mesh sieve (4) are circular, and the diameters of the 150-mesh sieve (3) and the 250-mesh sieve (4) are the same as the diameter of the 50mL centrifuge tube (5).
[0013] The connecting rod (2) has a threaded structure and a non-adhesive polytetrafluoroethylene coating (201) on its surface; the 150-mesh screen (3) and the 250-mesh screen (4) are detachably connected to the connecting rod (2) by a rotating buckle (202);
[0014] The top of the sealing cap (1) is provided with a leak-proof silicone pad (101).
[0015] Preferably, the rotating buckles (202) at both ends of the connecting rod (2) are aligned with the 150-mesh screen (3) and the 250-mesh screen (4) respectively, and fixed by rotating clockwise so that the 150-mesh screen (3) and the 250-mesh screen (4) are tightly connected to the connecting rod (2).
[0016] Preferably, the 150-mesh sieve (3) has an aperture of 90-110 μm, and the 250-mesh sieve (4) has an aperture of 50-65 μm.
[0017] Preferably, the 150-mesh sieve (3) and the 250-mesh sieve (4) are both made of metal.
[0018] By adopting the above technical solution, it can be seen that the present invention provides a device for collecting Echinococcus tapeworm eggs, which has the following beneficial effects compared with the prior art:
[0019] This invention utilizes a double-layer gradient sieve structure to achieve integrated high-efficiency filtration and targeted enrichment, taking into account the differences in physical characteristics between parasite eggs and impurities in fecal samples. The 150-mesh metal sieve intercepts large solid particles in the feces, initially removing fibers and food residues and reducing background interference. The 250-mesh metal sieve filters fine suspended matter, reducing water turbidity and minimizing the impact of bacterial clumps and cell debris, while also filtering larger parasite eggs, such as fertilized Ascaris eggs. Echinococcus tapeworm eggs are quickly sieved into the bottom of the centrifuge tube. Through a modular connecting rod and sealing cap design, the sieve assembly can be quickly embedded into a 50mL standard centrifuge tube, forming a closed filtration system. This eliminates the need for sample transfer, avoiding aerosol contamination and pathogen exposure risks, further ensuring the cleanliness of the experimental environment and operational safety. Attached Figure Description
[0020] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.
[0021] Figure 1 A schematic diagram of a device for collecting Echinococcus tapeworm eggs;
[0022] Figure 2 The image shows the microscopic examination results of the egg suspension obtained using the Echinococcus tapeworm egg filtration method of Example 1.
[0023] Figure 3 The image shows the microscopic examination results of the egg suspension obtained using the Echinococcus tapeworm egg filtration method of Comparative Example 1.
[0024] In this diagram, 1 represents a sealing cap, 101 represents a leak-proof silicone pad, 2 represents a connecting rod, 201 represents an anti-adhesion PTFE coating, 202 represents a rotating buckle, 3 represents a 150-mesh sieve, 4 represents a 250-mesh sieve, and 5 represents a 50mL centrifuge tube. Detailed Implementation
[0025] This invention provides a device for collecting Echinococcus tapeworm eggs, including a sealing cap (1), a connecting rod (2), a 150-mesh sieve (3), a 250-mesh sieve (4), and a 50 mL centrifuge tube (5).
[0026] See Figure 1A 150-mesh sieve (3) and a 250-mesh sieve (4) are respectively connected to the connecting rod (2). The 150-mesh sieve (3) is close to the upper end of the 50mL centrifuge tube (5), and the 250-mesh sieve (4) is close to the lower end of the 50mL centrifuge tube (5) and flush with the bottom of the connecting rod (2). The distance between the 150-mesh sieve (3) and the 250-mesh sieve (4) is 15-25mm. The length of the connecting rod (2) does not exceed the length of the 50mL centrifuge tube (5). Both (3) and 250 mesh screen (4) are circular. The diameters of 150 mesh screen (3) and 250 mesh screen (4) are the same as the diameter of 50mL centrifuge tube (5). The connecting rod (2) has a threaded structure and is coated with an anti-adhesion polytetrafluoroethylene coating (201) on its surface. The 150 mesh screen (3) and 250 mesh screen (4) are detachably connected to the connecting rod (2) by a rotating buckle (202). The top of the sealing cap (1) is provided with a leak-proof silicone pad (101).
[0027] In the specific implementation process, the rotating buckles (202) at both ends of the connecting rod (2) are aligned with the 150-mesh screen (3) and the 250-mesh screen (4) respectively, and fixed by rotating clockwise so that the 150-mesh screen (3) and the 250-mesh screen (4) are tightly connected to the connecting rod (2).
[0028] In this utility model, the 150-mesh sieve (3) and the 250-mesh sieve (4) are both made of metal; the aperture of the 150-mesh sieve (3) is preferably 90-110 μm, and more preferably 95-105 μm; the aperture of the 250-mesh sieve (4) is preferably 50-65 μm, and more preferably 55-60 μm.
[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0030] Example 1
[0031] Microscopic examination of feces containing Echinococcus granulosus eggs after filtration using an Echinococcus granulosus egg collection device.
[0032] like Figure 1As shown, this utility model embodiment provides a device for collecting Echinococcus granulosus eggs and its usage method. The rotating buckles (202) at both ends of the connecting rod (2), whose threaded structure has an anti-adhesion polytetrafluoroethylene coating (201), are aligned with a 150-mesh (100μm) metal sieve (3) and a 250-mesh (60μm) metal sieve (4), respectively, and fixed by clockwise rotation to ensure a tight connection between the sieve and the connecting rod. The assembled sieve assembly is vertically inserted into a centrifuge tube (5). A 10g fecal sample containing Echinococcus granulosus eggs is placed on the 150-mesh metal sieve (3), 40mL of physiological saline is added, and a sealing cap (1) with a leak-proof silicone pad (101) is placed on top. The sample is thoroughly mixed using a vortex mixer and allowed to stand for 10 minutes. The centrifuge tube is then transferred to a centrifuge and centrifuged at 3000r / min for 10 minutes. The assembled double-layer sieve assembly is then vertically removed from the 50mL centrifuge tube (5). Take 100 μL of the egg suspension for microscopic examination.
[0033] Experimental results: such as Figure 2 As shown, under an optical microscope, spherical, smooth embryonic membrane-bound, 30-40 μm diameter Echinococcus granulosus eggs can be seen.
[0034] Comparative Example 1
[0035] The difference between this comparative example and Example 1 is that Example 1 uses a double-layer screen assembly, while this comparative example uses a single-layer screen. The specific steps are as follows:
[0036] Take 10g of fecal sample containing Echinococcus multilocularis eggs and place it in a 50mL centrifuge tube. Add 40mL of physiological saline, vortex the sample thoroughly, and let it stand for 10 minutes. Then transfer the sample to a 200-mesh (70μm pore size) nylon sieve, place the sieve on a beaker, and grind and filter thoroughly using a grinding rod. Obtain an egg suspension. Take 100μL of the egg suspension for microscopic examination.
[0037] Experimental results: such as Figure 3 As shown, under an optical microscope, there are many residual impurities and numerous crystalline particles, indicating low sample purity.
[0038] As can be seen from the above embodiments and comparative examples, this utility model, through a double-layer gradient screen structure, achieves an integrated operation of efficient filtration and targeted enrichment, taking into account the differences in physical characteristics between parasite eggs and impurities in fecal samples. The 150-mesh metal screen intercepts large solid particles in the feces, initially removing fibers and food residues, and reducing background interference; the 250-mesh metal screen filters fine suspended matter, reducing water turbidity and minimizing the impact of bacterial clumps and cell debris, while also filtering larger parasite eggs, such as fertilized Ascaris eggs; Echinococcus tapeworm eggs are quickly sieved into the bottom of the tube using a centrifuge; through a modular connecting rod and sealing cap design, the screen assembly can be quickly embedded into a 50mL standard centrifuge tube, forming a closed filtration system. This eliminates the need for sample transfer, avoiding aerosol contamination and pathogen exposure risks, further ensuring the cleanliness of the experimental environment and operational safety.
[0039] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.
[0040] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A device for collecting Echinococcus tapeworm eggs, characterized in that, Includes a sealing cap (1), a connecting rod (2), a 150-mesh sieve (3), a 250-mesh sieve (4), and a 50 mL centrifuge tube (5); The 150-mesh sieve (3) and the 250-mesh sieve (4) are respectively connected to the connecting rod (2). The 150-mesh sieve (3) is close to the upper end of the 50mL centrifuge tube (5), and the 250-mesh sieve (4) is close to the lower end of the 50mL centrifuge tube (5) and is flush with the bottom of the connecting rod (2). The spacing between the 150-mesh sieve (3) and the 250-mesh sieve (4) is 15-25 mm; The length of the connecting rod (2) shall not exceed the length of the 50mL centrifuge tube (5); Both the 150-mesh sieve (3) and the 250-mesh sieve (4) are circular, and the diameters of the 150-mesh sieve (3) and the 250-mesh sieve (4) are the same as the diameter of the 50mL centrifuge tube (5). The connecting rod (2) has a threaded structure and a non-adhesive polytetrafluoroethylene coating (201) on its surface; the 150-mesh screen (3) and the 250-mesh screen (4) are detachably connected to the connecting rod (2) by a rotating buckle (202); The top of the sealing cap (1) is provided with a leak-proof silicone pad (101).
2. The Echinococcus tapeworm egg collection device according to claim 1, characterized in that, Align the rotating buckles (202) at both ends of the connecting rod (2) with the 150-mesh screen (3) and the 250-mesh screen (4) respectively, and rotate them clockwise to fix them so that the 150-mesh screen (3) and the 250-mesh screen (4) are tightly connected to the connecting rod (2).
3. The Echinococcus tapeworm egg collection device according to claim 1, characterized in that, The 150-mesh sieve (3) has an aperture of 90-110 μm, and the 250-mesh sieve (4) has an aperture of 50-65 μm.
4. The Echinococcus granulosus egg collection device according to any one of claims 1 to 3, characterized in that, Both the 150-mesh sieve (3) and the 250-mesh sieve (4) are made of metal.