Ultrasonic enzymolysis centrifugal device

Abstract

The utility model provides an ultrasonic enzymolysis centrifugal device which comprises a centrifugal turntable device and enzymolysis cups uniformly distributed on the centrifugal turntable device, and the centrifugal turntable device is also provided with an ultrasonic device on the lower side of the enzymolysis cups; the enzymolysis cup comprises a cup body and an ultrasonic transmission rod arranged in the center of the bottom of the cup body, the ultrasonic transmission rod is connected with the cup body through a rubber ring, the side, facing the ultrasonic device, of the ultrasonic transmission rod is of a hollow structure, and a vibration head of the ultrasonic device is clamped into the hollow structure of the ultrasonic transmission rod. The ultrasonic enzymolysis and centrifugation equipment has the following beneficial effects that the enzymolysis cup with the ultrasonic transmission rod is matched with the centrifugal turntable device with the ultrasonic device, so that the equipment with ultrasonic enzymolysis and centrifugation functions is obtained, and the technical problem of low transmission efficiency between an existing ultrasonic enzymolysis process and an existing centrifugation process is solved.

Description

Technical Field

[0001] This utility model relates to the field of enzymatic hydrolysis equipment technology, and in particular to an ultrasonic enzymatic hydrolysis centrifugation device. Background Technology

[0002] Ultrasonic enzymatic hydrolysis followed by centrifugation is a highly efficient process widely used in the extraction of biomolecules or cell disruption. Its core process typically involves using an ultrasonic disruptor to physically treat the sample at a specific frequency (e.g., 20-40kHz) and power range (100-1000W). This disrupts cell walls or membranes through cavitation, while simultaneously utilizing the directed catalytic action of enzymes (e.g., proteases, cellulases, or pectins). The reaction proceeds synergistically within a pH buffer system and a constant temperature environment (usually controlled at 30-50℃). The processing time is adjusted to 10-60 minutes depending on the material characteristics. Intermittent ultrasound (5s / 5s work / pause cycle) is used to prevent overheating and enzyme inactivation. After hydrolysis, a high-speed centrifuge is used at 4-10℃ with a centrifugal force of 8000-15000×g for 15-30 minutes to separate the solid and liquid layers. The supernatant is collected by siphon or pipetting and proceeds to subsequent purification steps, while the bottom precipitate is selectively retained or discarded based on the distribution of the target product.

[0003] However, conventional ultrasonic enzymatic hydrolysis and centrifugation are carried out in two separate devices, namely ultrasonic enzymatic hydrolysis equipment and centrifuge. In order to achieve automated production, a transmission device or pipeline is usually set between the two. However, whether it is transmission device or pipeline, it will inevitably waste some time and reduce work efficiency. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides an ultrasonic enzymatic hydrolysis centrifugation device, which solves the technical problem of low transmission efficiency between the existing ultrasonic enzymatic hydrolysis and centrifugation processes.

[0005] An ultrasonic enzymatic hydrolysis centrifugation device according to an embodiment of the present invention includes a centrifugal turntable device and enzymatic hydrolysis cups evenly distributed on the centrifugal turntable device. The centrifugal turntable device is also provided with an ultrasonic device located below the enzymatic hydrolysis cups.

[0006] The enzymatic hydrolysis cup includes a cup body and an ultrasonic transmission rod located at the center of the bottom of the cup body. The ultrasonic transmission rod is connected to the cup body by a rubber ring. The ultrasonic transmission rod is hollow on the side facing the ultrasonic device. The vibrating head of the ultrasonic device is inserted into the hollow structure of the ultrasonic transmission rod.

[0007] The technical principle of this utility model is as follows: In use, the bottom ultrasonic device directly transmits vibration to the ultrasonic transmission rod through the vibrating head, and then acts on the liquid in the enzymatic hydrolysis cup to accelerate enzymatic hydrolysis. After the enzymatic hydrolysis is completed, the centrifugation process is directly carried out through the centrifugal turntable device to achieve solid-liquid separation.

[0008] In this application, the ultrasonic device is placed at the bottom of the enzymatic hydrolysis cup, so that the ultrasonic device can be directly combined with the centrifugal turntable device. Compared with the traditional ultrasonic device that is inserted from the top, no additional support is needed to fix and move the ultrasonic device when realizing automated production.

[0009] This application uses a rubber ring to buffer the vibration generated by the vibrating head on the cup body, thereby reducing the risk of damage to the cup body.

[0010] Compared with the prior art, the present invention has the following advantages: by combining an enzymatic hydrolysis cup with an ultrasonic transmission rod with a centrifugal turntable with an ultrasonic device, a device with ultrasonic enzymatic hydrolysis and centrifugation is obtained, which solves the technical problem of low transmission efficiency between the existing ultrasonic enzymatic hydrolysis process and the centrifugation process.

[0011] Furthermore, the ultrasonic transmission rod is made of rubber.

[0012] Furthermore, the centrifugal turntable device includes a support frame and a turntable body disposed on the top of the support frame. A rotating motor is provided at the bottom center of the turntable body. A slide rail connects the rotating motor and the support frame. A lifting push rod is provided at the bottom of the rotating motor. The lifting push rod can push the rotating motor and the turntable body to move along the slide rail.

[0013] The ultrasonic devices are all mounted on a support frame, and the enzymatic hydrolysis cups are evenly distributed on the turntable. The turntable has a circular hole, through which the vibrating head of the ultrasonic device can pass and connect to the enzymatic hydrolysis cup.

[0014] By setting up a lifting push rod to drive the rotating motor and the turntable to rise, the ultrasonic device is separated from the turntable, which reduces the energy consumption of the rotating motor driving the turntable.

[0015] Furthermore, the bottom of the turntable is provided with an annular track, and support rods are evenly distributed around the lower side of the annular track. All the support rods are connected to the housing of the rotating motor, and the rotating shaft of the rotating motor is connected to the turntable in a transmission connection.

[0016] Furthermore, the turntable body is evenly distributed with locking positions, the locking positions are concave structures, the circular hole is located in the center of the locking position, and the enzymatic hydrolysis cup is installed in the locking position.

[0017] Furthermore, the bottom of the snap-fit ​​position has grooves evenly distributed around its circumference, and the bottom of the enzymatic hydrolysis cup has protrusions evenly distributed around its circumference, with each protrusion snapping into a groove for fixation.

[0018] Furthermore, the bottom of the snap-fit ​​position has through holes evenly distributed around its circumference, the support frame has spring structures corresponding to the through holes, and the bottom of the enzymatic hydrolysis cup has recesses evenly distributed around its circumference. Each spring structure passes through the through hole and snaps into a recess for fixation.

[0019] The ultrasonic vibration is buffered by a spring structure to reduce the damage to the internal connection structure of the centrifugal turntable device. It should be noted that although the ultrasonic device in this application is directly installed on the support frame, the use of rubber pads and other means to buffer the ultrasonic device when it is connected to other components is a conventional technical means in this field, and therefore is not reflected in this application.

[0020] Furthermore, the spring structure includes a spring body and a rubber body disposed on the top of the spring body, the bottom of the spring body is fixed on the support frame, and the rubber body is inserted into the recess. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the ultrasonic enzymatic hydrolysis centrifugation device according to Embodiment 1 of this utility model.

[0022] Figure 2 This is a top view of the turntable body in Embodiment 1 of this utility model.

[0023] Figure 3 This is a schematic diagram of the enzymatic hydrolysis cup connection structure of Embodiment 1 of this utility model.

[0024] Figure 4 This is a schematic diagram of the enzymatic hydrolysis cup connection structure in Embodiment 2 of this utility model.

[0025] Figure 5 This is a bottom view of the enzymatic hydrolysis cup in Embodiment 2 of this utility model.

[0026] In the above figures: 1. Enzyme hydrolysis cup; 11. Cup body; 111. Protrusion; 112. Depression; 12. Ultrasonic transmission rod; 121. Rubber ring; 2. Ultrasonic device; 21. Vibrating head; 3. Support frame; 31. Slide rail; 32. Spring structure; 321. Spring body; 322. Rubber body; 4. Turntable body; 41. Snap-fit ​​position; 411. Round hole; 412. Groove; 413. Through hole; 42. Circular track; 5. Rotating motor; 51. Support rod; 6. Lifting push rod. Detailed Implementation

[0027] The technical solution of this utility model will be further described below with reference to the accompanying drawings and embodiments.

[0028] Example 1

[0029] like Figure 1-3The ultrasonic enzymatic hydrolysis centrifuge device shown includes a centrifugal disc device and enzymatic hydrolysis cups 1 evenly distributed on the centrifugal disc device. An ultrasonic device 2 is also provided on the lower side of the enzymatic hydrolysis cups 1 on the centrifugal disc device. The ultrasonic device 2 in this application is a conventional device, such as consisting of an ultrasonic generator, a transducer, an amplitude transformer, and a vibrating head 21. The ultrasonic generator converts power frequency electricity (such as 220V / 50Hz) into high frequency electrical signals (usually 20kHz to 100kHz) to provide energy for the transducer. The transducer converts high frequency electrical energy into mechanical vibration and is the core component of ultrasonic energy conversion. The amplitude transformer amplifies the mechanical amplitude of the transducer and focuses the vibration energy to the vibrating head 21. The vibrating head 21 directly transmits ultrasonic energy to the working medium or workpiece.

[0030] like Figure 3 As shown, the enzymatic hydrolysis cup 1 includes a cup body 11 and an ultrasonic transmission rod 12 disposed at the center of the bottom of the cup body 11. A rubber ring 121 is injection molded between the ultrasonic transmission rod 12 and the cup body 11. The ultrasonic transmission rod 12 is hollow on the side facing the ultrasonic device 2. The vibrating head 21 of the ultrasonic device 2 is inserted into the hollow structure of the ultrasonic transmission rod 12. In this embodiment, the main body of the ultrasonic transmission rod 12 is made of metal, which can effectively transmit the vibration of the vibrating head 21.

[0031] like Figure 1 As shown, the centrifugal turntable device includes a support frame 3 and a turntable body 4 set on the top of the support frame 3. A rotating motor 5 is provided at the bottom center of the turntable body 4. The rotating shaft of the rotating motor 5 is connected to the turntable body 4 for transmission. A slide rail 31 is provided between the rotating motor 5 and the support frame 3. A lifting push rod 6 is provided at the bottom of the rotating motor 5. The lifting push rod 6 can push the rotating motor 5 and the turntable body 4 to move along the slide rail 31.

[0032] like Figure 2-3 As shown, the turntable body 4 is evenly distributed with snap-fit ​​positions 41. The snap-fit ​​positions 41 have a concave structure and a circular hole 411 is provided in the center of the snap-fit ​​position 41. The enzymatic hydrolysis cup 1 is installed in the snap-fit ​​position 41. The ultrasonic devices 2 are all installed on the support frame 3. The vibrating head 21 of the ultrasonic device 2 can pass through the circular hole 411 and connect to the enzymatic hydrolysis cup 1.

[0033] The specific locking position 41 has grooves 412 evenly distributed around its bottom circumference, and the enzyme digestion cup 1 has protrusions 111 evenly distributed around its bottom circumference. Each protrusion 111 is locked into a groove 412 for fixation. During centrifugation, the relative position of the enzyme digestion cup 1 in the locking position 41 can remain unchanged.

[0034] like Figure 1As shown, the bottom of the turntable body 4 is provided with an annular track 42, which allows the turntable body 4 to rotate along the annular track 42, improving the stability of the turntable body 4 when rotating; support rods 51 are evenly distributed around the lower side of the annular track 42, and all support rods 51 are connected to the housing of the rotating motor 5, forming a stable triangular structure, which can maintain the stability of the annular track 42.

[0035] Example 2

[0036] like Figure 4-5 As shown, the difference between this embodiment and embodiment 1 is that: the bottom of the snap-fit ​​position 41 has through holes 413 evenly distributed around the periphery, the support frame 3 is provided with spring structure 32 corresponding to the through holes 413, the bottom of the enzymatic hydrolysis cup 1 has recesses 112 evenly distributed around the periphery, and the recesses 112 and the protrusions 111 are misaligned. Each spring structure 32 passes through the through hole 413 and is snapped into a recess 112 for fixation. The specific spring structure 32 includes a spring body 321 and a rubber body 322 provided on the top of the spring body 321. The bottom of the spring body 321 is fixed on the support frame 3, and the rubber body 322 is snapped into the recess 112 for fixation.

[0037] Example 3

[0038] The difference between this embodiment and Embodiment 1 or 2 is that the ultrasonic transmission rod 12 is made of rubber, which can better wrap the vibrating head 21 of the ultrasonic device 2 compared to metal. However, it will absorb some vibration when transmitting vibration.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this utility model without departing from the spirit and scope of the technical solutions of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. An ultrasonic enzymatic hydrolysis centrifugation device, characterized in that: The device includes a centrifugal disc device and enzymatic hydrolysis cups evenly distributed on the centrifugal disc device. The centrifugal disc device is also equipped with an ultrasonic device located below the enzymatic hydrolysis cups. The enzymatic hydrolysis cup includes a cup body and an ultrasonic transmission rod located at the center of the bottom of the cup body. The ultrasonic transmission rod is connected to the cup body by a rubber ring. The ultrasonic transmission rod is hollow on the side facing the ultrasonic device. The vibrating head of the ultrasonic device is inserted into the hollow structure of the ultrasonic transmission rod.

2. The ultrasonic enzymatic hydrolysis centrifuge device as described in claim 1, characterized in that: The ultrasonic transmission rod is made of rubber.

3. The ultrasonic enzymatic hydrolysis centrifuge device as described in claim 1, characterized in that: The centrifugal turntable device includes a support frame and a turntable body disposed on the top of the support frame. A rotating motor is provided at the bottom center of the turntable body. A slide rail connects the rotating motor and the support frame. A lifting push rod is provided at the bottom of the rotating motor. The lifting push rod can push the rotating motor and the turntable body to move along the slide rail. The ultrasonic devices are all mounted on a support frame, and the enzymatic hydrolysis cups are evenly distributed on the turntable. The turntable has a circular hole, through which the vibrating head of the ultrasonic device can pass and connect to the enzymatic hydrolysis cup.

4. The ultrasonic enzymatic hydrolysis centrifuge device as described in claim 3, characterized in that: The bottom of the turntable is provided with an annular track, and support rods are evenly distributed around the lower side of the annular track. All the support rods are connected to the housing of the rotating motor, and the rotating shaft of the rotating motor is connected to the turntable in a transmission connection.

5. The ultrasonic enzymatic hydrolysis centrifuge device as described in claim 3, characterized in that: The turntable body is evenly distributed with locking positions, the locking positions are concave structures, the circular holes are located in the center of the locking positions, and the enzymatic cups are installed in the locking positions.

6. The ultrasonic enzymatic hydrolysis centrifuge device as described in claim 5, characterized in that: The bottom of the snap-fit ​​position has grooves evenly distributed around its circumference, and the bottom of the enzymatic hydrolysis cup has protrusions evenly distributed around its circumference. Each protrusion is snapped into a groove for fixation.

7. The ultrasonic enzymatic hydrolysis centrifuge device as described in claim 5, characterized in that: The bottom of the snap-fit ​​position has through holes evenly distributed around its circumference, and the support frame is provided with a spring structure corresponding to the through holes. The bottom of the enzymatic hydrolysis cup has recesses evenly distributed around its circumference, and each spring structure passes through the through hole and snaps into a recess for fixation.

8. The ultrasonic enzymatic hydrolysis centrifuge device as described in claim 7, characterized in that: The spring structure includes a spring body and a rubber body disposed on the top of the spring body. The bottom of the spring body is fixed on a support frame, and the rubber body is inserted into a recess.

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