A stem cell cryopreservation apparatus

Abstract

The utility model discloses a kind of stem cell cryopreservation equipment, it is related to stem cell freezing technical field, including jar body, the jar body top end is equipped with sealing cover, the jar body inside is provided with placing cylinder, the placing cylinder one side is equipped with opening, the opening out slidingly connected with sealing plate, the sealing plate one side is fixedly connected with slide bar, the sealing plate is slidingly connected with placing cylinder by slide bar, the placing cylinder one side is equipped with the sliding slot matched with it. The utility model is through being equipped with sealing cover, sealing plate and adjustable pressing plate structure, effectively realize the whole sealing in stem cell storage process, avoid refrigeration gas in operating process leakage, improve the operation safety, prevent cold air and operator direct contact, reduce frostbite risk, utilize pressing plate and elastic sheet linkage extrude residual gas in placing cylinder, and it is backflow to jar body inside, form gas circulation reuse mechanism, reduce gas consumption rate, improve gas use efficiency.

Description

Technical Field

[0001] This utility model relates to the field of stem cell cryopreservation technology, specifically a stem cell cryopreservation device. Background Technology

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capacity. They can continuously replicate through their own division and differentiate into at least one type of highly specialized daughter cells. They are widely used in many cutting-edge biomedical fields such as regenerative medicine, tissue engineering, and gene therapy. Due to the important value of stem cells in scientific research and clinical practice, after extraction and culture, they usually need to be stored for a long time in an extremely low temperature environment to ensure their bioactivity and functional integrity for future use. Liquid nitrogen, with its ultra-low temperature characteristic of -196°C, has become the preferred medium for stem cell storage. It can effectively inhibit cell metabolic activities, prevent cell apoptosis or functional degeneration, thereby achieving long-term quiescent preservation of stem cells.

[0003] In most existing stem cell storage technologies, the process of retrieving stem cells causes a large amount of gas to leak out, and the staff's hands come into contact with this gas, which affects the safety and effectiveness of the equipment during use. Utility Model Content

[0004] Based on this, the purpose of this utility model is to provide a stem cell cryopreservation device to solve the technical problem that the device causes a large amount of gas to leak out during the stem cell extraction process, and the staff's hands come into contact with this gas, which affects the safety and effectiveness of the device during use.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a stem cell cryopreservation device, comprising a tank, a sealing cap installed at the top of the tank, a placement cylinder disposed inside the tank, an opening on one side of the placement cylinder, a sealing plate slidably connected to the opening, a sliding rod fixedly connected to one side of the sealing plate, the sealing plate being slidably connected to the placement cylinder via the sliding rod, and a sliding groove cooperating therewith being provided on one side of the placement cylinder.

[0006] By adopting the above technical solution, and by setting up a sealing cover, sealing plate, and adjustable pressure plate structure, the entire process of stem cell storage is effectively sealed, avoiding leakage of refrigerated gas during operation, improving operational safety, preventing direct contact between cold air and the operator, and reducing the risk of frostbite. The pressure plate and elastic sheet work together to squeeze the residual gas inside the storage container and return it to the inside of the tank, forming a gas recycling mechanism, reducing gas consumption rate, improving gas utilization efficiency, and having a good energy-saving effect. The sliding sealing plate and pluggable fixing structure facilitate quick removal or replacement of the storage container and stem cell samples. The bolts and bolt holes are firmly positioned, improving structural stability and ensuring safety during handling and operation. Through the synergistic buffer design of the pressure plate and elastic sheet, direct compression of the stem cells inside the storage box can be avoided, ensuring that the physical stability and activity of the samples are not damaged during movement, sealing, and gas replacement.

[0007] Furthermore, a storage box is provided inside the placement cylinder, and a positioning rod is fixedly connected to the top of the storage box.

[0008] By adopting the above technical solution, the storage box effectively stores stem cells and achieves multi-layered protection.

[0009] Furthermore, the length of the positioning rod is greater than the radius of the placement cylinder, and a fixing groove that matches the top of the placement cylinder is provided.

[0010] By adopting the above technical solution, the fixed groove effectively secures the positioning rod.

[0011] Furthermore, a pressure plate is slidably connected inside the placement cylinder, and multiple sets of telescopic rods are provided on the side of the pressure plate.

[0012] By adopting the above technical solution, the friction between the pressure plate and the inner wall of the placement cylinder is increased by setting the telescopic rod, thus preventing the pressure plate from falling off the placement cylinder.

[0013] Furthermore, an elastic sheet is provided at the center of the pressure plate, and the elastic sheet is a deformable elastic structure.

[0014] By adopting the above technical solution, the elastic sheet is a deformable elastic structure, which allows the elastic sheet to deform after contacting the storage box, so that the entire pressure plate can smoothly reach the bottom of the placement cylinder.

[0015] Furthermore, a fixing plate is fixedly connected to the top of the tank, a fixing bolt is threaded to one side of the fixing plate, and a bolt hole that matches the fixing plate is opened on one side of the bottom of the placement cylinder.

[0016] By adopting the above technical solution, the fixing bolts and bolt holes effectively secure the placement cylinder, preventing it from contacting the ground and causing contamination.

[0017] In summary, this utility model has the following beneficial effects: By setting a sealing cover, sealing plate, and adjustable pressure plate structure, this utility model effectively achieves full sealing during the stem cell storage process, avoiding leakage of refrigerated gas during operation, improving operational safety, preventing direct contact between cold air and the operator, and reducing the risk of frostbite. The pressure plate and elastic sheet work together to squeeze the residual gas inside the placement cylinder and return it to the inside of the tank, forming a gas recycling mechanism, reducing gas consumption rate, improving gas utilization efficiency, and having a good energy-saving effect. The sliding sealing plate and pluggable fixing structure facilitate quick removal or replacement of the placement cylinder and stem cell samples. The bolts and bolt holes are firmly positioned, improving structural stability and ensuring safety during handling and operation. Through the synergistic buffer design of the pressure plate and elastic sheet, direct compression of the stem cells inside the storage box can be avoided, ensuring that the physical stability and activity of the samples are not damaged during movement, sealing, and gas replacement. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a partial structural schematic diagram of the present invention;

[0020] Figure 3 This is a partial exploded view of the structure of this utility model;

[0021] Figure 4 This is a partial structural diagram from a first-view perspective of the present invention;

[0022] Figure 5 This is a partial structural diagram from a second perspective of the present invention.

[0023] In the diagram: 1. Tank body; 2. Placement cylinder; 3. Storage box; 4. Positioning rod; 5. Opening; 6. Sealing plate; 7. Sliding rod; 8. Sliding groove; 9. Pressure plate; 10. Elastic sheet; 11. Telescopic rod; 12. Bolt hole; 13. Fixing plate; 14. Fixing bolt; 15. Sealing cover; 16. Fixing groove. Detailed Implementation

[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0025] The embodiments of this utility model will be described below based on its overall structure.

[0026] A stem cell cryopreservation device, such as Figures 1-5 As shown, it includes a tank body 1, a sealing cap 15 installed on the top of the tank body 1, a placement cylinder 2 inside the tank body 1, an opening 5 on one side of the placement cylinder 2, a sealing plate 6 slidably connected to the opening 5, a sliding rod 7 fixedly connected to one side of the sealing plate 6, the sealing plate 6 slidably connected to the placement cylinder 2 through the sliding rod 7, a sliding groove 8 that cooperates with it on one side of the placement cylinder 2, a storage box 3 inside the placement cylinder 2, and a positioning rod 4 fixedly connected to the top of the storage box 3;

[0027] Specifically, the operator first places the stem cells to be stored into the storage box 3, which is then placed inside the placement cylinder 2 to form the first preliminary sealed unit. Then, the entire placement cylinder 2, together with the storage box 3, is inserted into the tank 1. At this time, the opening 5 on the outer wall of the placement cylinder 2 has not yet been covered by the sealing plate 6. The tank 1 is pre-filled with low-temperature inert gas, which can directly contact the stem cells in the placement cylinder, thereby quickly performing gas replacement or low-temperature refrigeration pretreatment without affecting the activity of the stem cells.

[0028] Please see Figures 1-5 The length of the positioning rod 4 is greater than the radius of the placement cylinder 2. The top of the placement cylinder 2 is provided with a fixing groove 16 that matches it. The pressure plate 9 is slidably connected inside the placement cylinder 2. Multiple sets of telescopic rods 11 are provided on the side of the pressure plate 9. An elastic sheet 10 is provided in the center of the pressure plate 9. The elastic sheet 10 is a deformable elastic structure. Specifically, when the pressure plate 9 is operated to perform the pressure drop process, the pressure plate 9 is initially located at the top inside the placement cylinder 2. The operator uses a tool to press the pressure plate 9, causing it to move downwards along with the elastic sheet 10. The elastic sheet 10 is made of flexible material and has good deformation ability. When it contacts the top of the storage box 3, it can automatically deform and buffer to ensure that its downward movement proceeds smoothly without damaging the storage box structure or stem cell sample.

[0029] During this process, the pressure plate 9 compresses the inside of the placement cylinder 2, causing the residual gas inside to be expelled into the space of the tank 1, preventing the gas from leaking out through the opening 5. At the same time, it can effectively reduce the contact between the gas and the operator's hands, improving safety and energy saving.

[0030] Please see Figure 1 and Figure 2A fixing plate 13 is fixedly connected to the top of the tank body 1. A fixing bolt 14 is threadedly connected to one side of the fixing plate 13. A bolt hole 12 is opened on one side of the bottom of the placement cylinder 2 to match it. Specifically, the operator can lift the placement cylinder 2 out of the tank body 1 as a whole. At this time, the bolt hole 12 is aligned with the fixing bolt 14 on the upper fixing plate 13. After screwing in the fixing bolt 14, the placement cylinder 2 can be stably locked at the top of the tank body 1. Lateral sealing is achieved by the sealing plate 6. The pressure plate 9 forms a vertical block on the placement cylinder 2 at the top, further ensuring that the internal low temperature gas will not leak in large quantities during handling or sample removal.

[0031] The working principle of this utility model is as follows: When in use, the operator first puts the stem cells to be stored into the storage box 3, and then the storage box 3 is placed inside the placement cylinder 2 to form the first preliminary closed unit. Then, the entire placement cylinder 2 together with the storage box 3 inside is inserted into the tank body 1. At this time, the opening 5 on the outer wall of the placement cylinder 2 has not yet been covered by the sealing plate 6. The tank body 1 has been pre-filled with low temperature inert gas, which can directly contact the stem cells in the placement cylinder, so as to quickly perform gas replacement or low temperature refrigeration pretreatment without affecting the activity of stem cells.

[0032] Next, the sealing cap 15 is fastened to the top of the tank 1 to create a completely sealed storage environment to achieve the overall low-temperature sealed preservation effect. At this time, in order to further reduce excessive leakage of gas in the tank due to personnel operation, the pressure plate 9 can be operated to perform the pressure drop process. The pressure plate 9 is initially located at the top of the placement cylinder 2. The operator uses a tool to press the pressure plate 9, causing it and the elastic sheet 10 to move downwards gradually. The elastic sheet 10 is made of flexible material and has good deformation ability. When it contacts the top of the storage box 3, it can automatically deform and buffer to ensure that its downward movement is smooth without damaging the storage box structure or stem cell sample.

[0033] During this process, the pressure plate 9 compresses the inside of the placement cylinder 2, causing the residual gas inside to be expelled into the space of the tank 1, preventing the gas from leaking out through the opening 5. At the same time, it can effectively reduce the contact between the gas and the operator's hands, improving safety and energy saving.

[0034] When stem cells need to be extracted, the operator slides the fixed rod 4 along the slide groove 8, driving the slide rod 7 to move the sealing plate 6 horizontally to cover the opening 5, thereby completely sealing the outside of the placement cylinder 2 and preventing the low-temperature gas from escaping to the external environment during the extraction process. After sealing, the operator can lift the placement cylinder 2 out of the tank 1. At this time, the bolt hole 12 is aligned with the fixing bolt 14 on the upper fixed plate 13. After screwing in the fixing bolt 14, the placement cylinder 2 can be stably locked at the top of the tank 1. The sealing plate 6 achieves lateral sealing, and the pressure plate 9 forms a vertical block on the placement cylinder 2 at the top, further ensuring that the internal low-temperature gas will not leak in large quantities during handling or sample removal.

[0035] Finally, the pressure plate 9 can be pressed again to drive the elastic plate 10 to squeeze the residual gas, so that it returns to the cavity of the tank 1 for circulation and storage, forming a closed-loop system. This reduces the consumption of cryogenic gas, improves the efficiency of use, and effectively avoids frostbite or environmental pollution caused by cold gas.

[0036] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. A stem cell cryopreservation device, comprising a tank (1), characterized in that: The top of the tank (1) is fitted with a sealing cap (15). Inside the tank (1) is a placement cylinder (2). An opening (5) is provided on one side of the placement cylinder (2). A sealing plate (6) is slidably connected to the opening (5). A sliding rod (7) is fixedly connected to one side of the sealing plate (6). The sealing plate (6) is slidably connected to the placement cylinder (2) through the sliding rod (7). A sliding groove (8) is provided on one side of the placement cylinder (2) to cooperate with it.

2. The stem cell cryopreservation device according to claim 1, characterized in that: The placement tube (2) is provided with a storage box (3) inside, and a positioning rod (4) is fixedly connected to the top of the storage box (3).

3. The stem cell cryopreservation device according to claim 2, characterized in that: The length of the positioning rod (4) is greater than the radius of the placement tube (2), and the top of the placement tube (2) is provided with a fixing groove (16) that matches it.

4. The stem cell cryopreservation device according to claim 1, characterized in that: The placement cylinder (2) is slidably connected to a pressure plate (9), and multiple sets of telescopic rods (11) are provided on the side of the pressure plate (9).

5. The stem cell cryopreservation device according to claim 4, characterized in that: The pressure plate (9) has an elastic sheet (10) at its center, and the elastic sheet (10) is a deformable elastic structure.

6. The stem cell cryopreservation device according to claim 1, characterized in that: The top of the tank (1) is fixedly connected to a fixing plate (13), and a fixing bolt (14) is threadedly connected to one side of the fixing plate (13). A bolt hole (12) is provided on one side of the bottom of the placement cylinder (2) to cooperate with it.

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