A special mold for zebrafish larvae drug toxicology experiment and high-throughput drug screening

Abstract

The utility model relates to a medicine screening dosing mould technical field, concretely relates to a zebra fish fry medicine toxicology experiment and high throughput medicine screening special mould of dosing, including fixed plate, a plurality of fixed slots are set up in fixed plate top, the filter screen pipe for draining zebra fish fry is placed in fixed slot, the fixed plate top is opened to fixed slot side wall and is equipped with the water injection groove, the fixed plate top still is opened to fixed slot side wall and is equipped with the dosing groove, fixed zebra fish fry drug toxicology experiment and high throughput medicine screening special mould of dosing in this, set up filter screen pipe and fixed plate, after zebra fish fry surface moisture drains, place filter screen pipe in fixed slot, inject water inwards, stop water injection when infrared sensor detects that water level reaches predetermined height, again pass dosing groove and inject medicine in it, and this kind of mould is simple to operate, occupies small, does not waste time and reagent, and can reduce experimental error.

Description

Technical Field

[0001] This utility model relates to the field of drug screening and administration mold technology, and in particular to a special mold for drug toxicology experiments and high-throughput drug screening and administration in zebrafish juveniles. Background Technology

[0002] Zebrafish are the third largest vertebrate model organism after mice and rats, and are widely used in many fields such as developmental biology, genetics, basic medicine, pharmacology, toxicology, drug development, and ecological environment assessment. Zebrafish can be effectively used for drug toxicology experiments and high-throughput drug screening. Currently, when using juvenile zebrafish for related experiments, it is necessary to manually drain the water from the surface of the juveniles to reduce drug concentration errors. However, manual draining has a large margin of error, and no suitable drug delivery mold has been found yet. The operation is relatively cumbersome, time-consuming, and may waste reagents. This mold is suitable for using juvenile zebrafish for drug toxicology experiments and high-throughput drug screening. It has the advantages of simple operation, small footprint, no waste of time and reagents, and reduced experimental errors.

[0003] When zebrafish juveniles are drained manually without a suitable drug administration mold, problems such as large drainage errors, cumbersome and time-consuming operation, and waste of reagents occur. In view of this, a special mold for drug toxicology experiments and high-throughput drug screening and administration to zebrafish juveniles is provided. Summary of the Invention

[0004] The main purpose of this invention is to provide a special mold for drug toxicology experiments and high-throughput drug screening in zebrafish juveniles, in order to solve the problems mentioned in related technologies, such as large drainage errors, cumbersome and time-consuming operation, and waste of reagents when zebrafish juveniles are drained manually without a suitable drug administration mold.

[0005] To achieve the above objectives, according to one aspect of the present invention, a special mold for drug toxicology experiments and high-throughput drug screening and administration of zebrafish juveniles is provided, comprising a fixing plate, a plurality of fixing grooves on the top of the fixing plate, a filter tube for draining zebrafish juveniles is placed in the fixing groove, a water injection groove is provided through the side wall of the fixing groove on the top of the fixing plate, and a drug administration groove is also provided through the side wall of the fixing groove on the top of the fixing plate, and an infrared sensor is fixedly installed on the side wall of the fixing groove.

[0006] As a preferred embodiment of the present invention, the filter tube includes a screen, and several support rods are fixedly arranged in a ring array near the top of the screen, and several protective rings are fixedly arranged longitudinally around the support rods.

[0007] As a preferred embodiment of this utility model, the support rod and the protective ring form a frame, and the frame is made of polypropylene. The screen mesh has a diameter of 200 μm and is made of nylon.

[0008] As a preferred embodiment of this utility model, the filter tube has a height of 4cm and a radius of 1.4cm.

[0009] As a preferred embodiment of this utility model, the height of the fixing groove is 4cm and the radius of the fixing groove is 1.8cm.

[0010] As a preferred embodiment of this utility model, one end of the water injection tank is connected to a water pipe, and one end of the drug administration tank is provided with a drug administration device, which is used to inject drugs into the drug administration tank.

[0011] As a preferred embodiment of this invention, the infrared sensor is positioned below the water injection tank.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] This zebrafish juvenile drug toxicology experiment and high-throughput drug screening dosing mold is equipped with a filter tube and a fixing plate. After the zebrafish juveniles drain their surface water, the filter tube is placed in the fixing tank, and water is poured in. When the infrared sensor detects that the water level has reached the predetermined height, the water filling stops, and then the drug is injected into the drug tank. This mold is simple to operate, occupies little space, does not waste time and reagents, and can reduce experimental errors. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the drug screening and administration mold in a preferred embodiment of the present invention.

[0015] Figure 2 This is a cross-sectional schematic diagram of a drug screening and administration mold in a preferred embodiment of the present invention.

[0016] Figure 3 This is a schematic diagram of the filter tube in a preferred embodiment of the present invention.

[0017] Figure 4 This is a connection circuit diagram in a preferred embodiment of the present invention.

[0018] Illustration:

[0019] 1. Fixing plate; 2. Fixing groove; 3. Filter screen tube; 4. Water injection tank; 5. Drug administration tank; 6. Infrared sensor; 31. Screen; 32. Support rod; 33. Protective ring;

[0020] 7. Water pump; 9. Timer; 10. Dosing device. Detailed Implementation

[0021] To further illustrate the technical means and effects adopted by this utility model in order to achieve the intended utility model purpose, the following detailed description of the specific implementation methods, structure, features and effects of this utility model is provided in conjunction with the accompanying drawings and preferred embodiments.

[0022] Please see Figures 1-4 As shown, the purpose of this embodiment is to provide a special mold for drug toxicology experiments and high-throughput drug screening and administration of zebrafish juveniles, including a fixing plate 1 with a height of 5cm. The top of the fixing plate 1 has several fixing grooves 2, and a filter tube 3 for draining zebrafish juveniles is placed in the fixing grooves 2. A water injection groove 4 is opened through the side wall of the fixing groove 2 from the top of the fixing plate 1. A drug administration groove 5 is also opened through the side wall of the fixing groove 2 from the top of the fixing plate 1. An infrared sensor 6 is fixedly installed on the side wall of the fixing groove 2.

[0023] The filter tube 3 includes a screen 31, and several support rods 32 are fixedly arranged in a ring array near the top of the screen 31. Several protective rings 33 are fixedly arranged longitudinally around the support rods 32.

[0024] The support rod 32 and the protective ring 33 form a frame, and the frame is made of polypropylene. The screen 31 has a pore size of 200um and is made of nylon.

[0025] The filter tube 3 has a height of 4cm and a radius of 1.4cm.

[0026] The height of the fixing groove 2 is 4cm, and the radius of the fixing groove 2 is 1.8cm.

[0027] One end of the water injection tank 4 is connected to a water pipe, and the other end of the water pipe is connected to a water source. Water is injected into the water injection tank 4 or stopped by controlling the start and stop of the water injection pump 7. One end of the drug administration tank 5 is equipped with a drug administration device 10, which is preferably an electric piston injector. The electric piston injector is purchased from NICHIRYO. The electric piston injector drives the push rod power head through a motor to transmit the required pressure to the injector. The drug is injected in this way. The start and stop of the drug administration device 10 is controlled by a double-contact normally open switch K1, and the duration of drug injection into the drug administration tank 5 by the piston injector is controlled by a timer 9.

[0028] Infrared sensor 6 is located below water tank 4.

[0029] Water pump 7 injects water into fixed tank 2 through water injection tank 4. Infrared sensor 6 monitors the liquid level. When the liquid level reaches the predetermined height, water injection stops, and drug delivery device 10 administers the drug. Specifically, the connection circuit diagram of water pump 7, infrared sensor 6, and drug delivery device 10 is shown below. Figure 4 As shown;

[0030] Among them, the negative terminal of the power supply VCC is connected to an infrared sensor 6, one end of the infrared sensor 6 is connected to a double-contact normally open switch K1, one end of the double-contact normally open switch K1 is connected to a water pump 7 and a drug delivery device 10 respectively, and the water pump 7 and the drug delivery device 10 are connected in parallel, and the on and off of the water pump 7 and the drug delivery device 10 are controlled by the double-contact normally open switch K1.

[0031] A timer 9 is connected to the common node of the water pump 7 and the drug delivery device 10. The other end of the timer 9 is connected to the positive terminal of the power supply VCC.

[0032] In practical use, zebrafish fry are placed on screen 31 and drained of surface moisture. Then, filter tube 3 is placed in fixed tank 2. Water is injected into fixed tank 2 through water injection tank 4. When infrared sensor 6 detects that the liquid level has reached the predetermined height, water injection is stopped. At the same time, medicine is pushed into drug delivery tank 5 through syringe, so that the medicine enters fixed tank 2 to administer medicine to zebrafish fry.

[0033] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to preferred embodiments, it is not intended to limit the present utility model. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present utility model. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model shall still fall within the scope of the present utility model.

Claims

1. A special mold for drug toxicology experiments and high-throughput drug screening in zebrafish juveniles, comprising a fixing plate (1), wherein the top of the fixing plate (1) is provided with a plurality of fixing grooves (2), characterized in that, The fixed groove (2) contains a filter tube (3) for draining zebrafish fry. The top of the fixed plate (1) has a water injection groove (4) that extends through the side wall of the fixed groove (2). The top of the fixed plate (1) also has a drug administration groove (5) that extends through the side wall of the fixed groove (2). An infrared sensor (6) is fixedly installed on the side wall of the fixed groove (2).

2. The zebrafish juvenile drug toxicology experiment and high-throughput drug screening dosing mold according to claim 1, characterized in that, The filter tube (3) includes a screen (31), and a number of support rods (32) are fixedly arranged in a ring array near the top of the screen (31) and a number of protective rings (33) are fixedly arranged longitudinally around the support rods (32).

3. The zebrafish juvenile drug toxicology experiment and high-throughput drug screening dosing mold according to claim 2, characterized in that, The support rod (32) and the protective ring (33) form a frame, and the frame is made of polypropylene. The screen (31) has a pore size of 200um and is made of nylon.

4. The zebrafish juvenile drug toxicology experiment and high-throughput drug screening dosing mold according to claim 1, characterized in that, The filter tube (3) has a height of 4cm and a radius of 1.4cm.

5. The zebrafish juvenile drug toxicology experiment and high-throughput drug screening dosing mold according to claim 1, characterized in that, The height of the fixing groove (2) is 4cm and the radius of the fixing groove (2) is 1.8cm.

6. The zebrafish juvenile drug toxicology experiment and high-throughput drug screening dosing mold according to claim 1, characterized in that, One end of the water injection tank (4) is connected to a water pipe, and one end of the drug administration tank (5) is provided with a drug administration device (10), which is used to inject drugs into the drug administration tank (5).

7. The zebrafish juvenile drug toxicology experiment and high-throughput drug screening dosing mold according to claim 1, characterized in that, The infrared sensor (6) is located below the water injection tank (4).

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