Microbiological evidence collection and preservation kit
By using a combined cannula structure and soluble cotton fiber design, the problems of difficult separation of the swab head from the stick, bacterial contamination, and the impact of cotton fibers on DNA extraction were solved, achieving efficient biological sample collection and preservation and improving DNA extraction efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HENAN ZHENGDA WUDUO IND TECHNOLOGY CO LTD
- Filing Date
- 2025-04-08
- Publication Date
- 2026-06-02
AI Technical Summary
In current biological evidence collection processes, the swab tip and swab stick are not easily separated, resulting in the loss of trace biological samples. Bacteria are introduced during the swab wiping process, cotton fibers affect DNA extraction, and cotton fibers are insoluble in lysis buffer, requiring additional removal steps, which affects DNA separation efficiency.
The device employs a tubular assembly structure, including an inner filter tube, barbs, spring clips, a fiber fixing sleeve, and soluble cotton fibers. Cotton fibers are collected by inserting the barbs into the gaps of the fiber fixing sleeve. The inner filter tube preserves the sample. The cotton fibers are soluble in a high-temperature lysis solution. The fiber fixing sleeve is made of silicone to adhere to the surface of the object. Nano-silver particles are sprayed onto the cotton fibers for antibacterial properties.
It improves the efficiency of biological sample extraction, avoids sample detachment during transportation, ensures that DNA content is not reduced, simplifies the DNA extraction process, and reduces sample loss and vector removal steps.
Smart Images
Figure CN224313520U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological sample extraction technology, specifically a kit for collecting and preserving trace biological evidence. Background Technology
[0002] With the development of DNA technology, extracting and examining biological samples left at crime scenes by suspects, and then identifying the perpetrator through DNA testing, has become a key technology for public security organs to solve various cases. According to Locard's theory of substance transfer, as long as objects come into contact, trace amounts of substances will be transferred between their surfaces. Therefore, every crime scene will leave behind biological samples of the perpetrator. Investigators need to collect and preserve these trace biological samples for DNA testing and identification in a DNA laboratory. This collection and preservation process requires the use of specialized trace biological evidence extraction kits.
[0003] Trace biological evidence is characterized by its low content, difficulty in extraction, and ease of loss, becoming a stumbling block to solving cases.
[0004] First, the cotton swabs used for collecting biological evidence currently have cotton fibers firmly wrapped around the swab stick. When laboratory personnel cut the cotton fibers with trace biological samples attached to the swab head, the fibers are not easily separated from the swab stick completely, and the cutting and tearing process can cause the trace biological samples to fall off and be lost.
[0005] Second, when biological samples are collected using existing biological evidence collection swabs, the collected biological samples are loosely attached to the surface of the swab tip. During the process of moving, transporting, and handing over the swab after it is placed in the evidence packaging bag, a small amount of biological sample loosely attached to the surface of the swab tip may fall off.
[0006] Third, after biological samples are collected by swabs and placed in evidence packaging bags, bacteria and other microorganisms from the environment are introduced during the swabbing process, which can easily cause the biological samples to be decomposed by microorganisms, resulting in a decrease in DNA content.
[0007] Fourth, in the DNA extraction stage, because cotton fibers are insoluble in the lysis buffer, the testing process needs to add a "carrier removal" step to remove the cotton fibers in order to avoid affecting subsequent DNA extraction, elution, purification, and preservation.
[0008] Fifth, because cotton fibers are insoluble in the lysis buffer, cotton fibers will carry some detached cells during lysis, affecting the full contact between the lysis buffer and the detached cells, resulting in incomplete lysis and hindering DNA separation. Utility Model Content
[0009] (a) Technical problems to be solved
[0010] To address the shortcomings of existing technologies, this utility model provides a solution to the problem that laboratory personnel often have difficulty separating the swab tip from the swab body when cutting off biological samples attached to the swab tip during traditional collection, delivery, and testing processes, resulting in the loss of trace biological evidence.
[0011] (II) Technical Solution
[0012] To achieve the above objectives, this utility model provides the following technical solution: a micro-biological evidence collection and preservation kit, including an outer tube, on which a sleeve assembly is provided. The sleeve assembly is used to collect and preserve cotton fibers with attached biological samples. The sleeve assembly includes an inner filter tube, a barb, a spring clip, a first groove, an oblique conical surface, a cotton swab, a fiber fixing sleeve, cotton fibers, and a sealing ring.
[0013] In the sleeve assembly, the cap is threaded onto the outer sleeve, the bottom of the outer sleeve stores desiccant, a connecting strap is fixedly connected between the outer sleeve and the cap, the inner filter tube is set on the protrusion inside the outer sleeve, and a filter screen mechanism is provided on the bottom wall of the inner filter tube.
[0014] Preferably, the barb is fixedly connected to the top wall of the lid, and the first groove is opened at the lower edge of the lid.
[0015] Preferably, the two spring clips are fixedly connected to the inner walls of the two sides of the first groove, and each spring clip has a positioning groove integrally formed.
[0016] Preferably, both spring cards have an integrally formed arc surface, and the cotton swab is disposed between the two positioning grooves.
[0017] Preferably, the fiber fixing sleeve is fixedly attached to one end of the cotton swab stick, and the fiber fixing sleeve is starfruit-shaped.
[0018] Preferably, the cotton fiber is sleeved on the fiber fixing sleeve, the oblique conical surface is opened at the opening of the outer sleeve, and the second groove is provided with an integrally formed conical protrusion adapted to the oblique conical surface.
[0019] Preferably, a second groove is provided on the conical protrusion, and a sealing ring is disposed in the second groove. The cotton fiber is a soluble fiber that can be completely dissolved in the high-temperature pyrolysis liquid.
[0020] (III) Beneficial Effects
[0021] Compared with existing technologies, this utility model provides a kit for collecting and preserving trace biological evidence, which has the following beneficial effects:
[0022] 1. This micro-biological evidence collection and preservation kit uses barbs that fit perfectly into the gaps of the fiber fixing sleeve, allowing the inner filter tube to collect the biological samples completely. This improves the efficiency of biological sample extraction and avoids the problem of difficulty in separating the swab head from the swab body when laboratory personnel cut off the biological samples attached to the swab head in traditional methods.
[0023] 2. This micro-biological evidence collection and preservation kit features a silicone fiber retainer, which allows the fiber retainer to better adhere to the surface of the object when the swab is used to collect biological samples. This avoids the problem of low sampling efficiency caused by the traditional method where the swab is too hard and the contact area with the object is too small.
[0024] 3. This micro-biological evidence collection and preservation kit, through its designed fiber fixing sleeve, allows cotton fibers to be evenly wrapped around the front three-quarters of the sleeve's surface. Scissors can be quickly inserted into the rear three-quarters of the pre-reserved gap to cut the attached cotton fibers. The soluble fibers can be completely dissolved in the high-temperature lysis solution. The cotton fibers are coated with nano-silver particles, which have an antibacterial effect. This avoids the problem of traditional methods where biological samples are swabbed with cotton swabs and placed in evidence packaging bags, which can easily lead to the decomposition of biological samples by microorganisms due to the introduction of bacteria and other microorganisms from the environment during the swabbing process, resulting in a decrease in DNA content.
[0025] 4. This micro-biological evidence collection and preservation kit, through its outer tube and inner filter tube, can effectively preserve the cotton fibers containing biological samples, avoiding the problem of biological samples easily falling off the surface of the swab head during movement, transportation, and handover after the swab is placed in the evidence packaging bag.
[0026] 5. This micro-biological evidence collection and preservation kit uses soluble cotton fibers that can be completely dissolved in the high-temperature lysis buffer. This avoids the problem in traditional methods where, during DNA extraction, cotton fibers are insoluble in the lysis buffer, requiring a "carrier removal" step to remove the cotton fibers. During lysis, the cotton fibers may trap some detached cells, leading to DNA loss during the "carrier removal" step (separation and removal of cotton fibers), thus affecting the progress of the case. Attached Figure Description
[0027] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0028] Figure 2 This is a schematic diagram of the internal filter tube structure of this utility model;
[0029] Figure 3 This is a schematic diagram of the fiber fixing sleeve structure of this utility model;
[0030] Figure 4This is a schematic diagram of the positioning groove structure of this utility model.
[0031] In the diagram: 1. Outer tube; 2. Cap; 3. Connecting strap; 4. Inner filter tube; 5. Sealing ring; 6. Barb; 7. Spring clip; 8. Positioning groove; 9. Arc surface; 10. First groove; 11. Sloping conical surface; 12. Cotton swab; 13. Fiber fixing sleeve; 14. Cotton fiber; 15. Second groove. Detailed Implementation
[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.
[0033] Please see Figure 1-4 This utility model provides a new technical solution: a micro biological evidence collection and preservation kit, including an outer tube 1, on which a sleeve assembly is provided. The sleeve assembly is used to collect and preserve cotton fibers with biological samples attached. The sleeve assembly includes an inner filter tube 4, a barb 6, a spring card 7, a first groove 10, an oblique conical surface 11, a cotton swab 12, a fiber fixing sleeve 13, cotton fibers 14, and a sealing ring 5.
[0034] In the sleeve assembly, the cover 2 is threaded onto the outer sleeve 1. The bottom of the outer sleeve 1 stores desiccant. A connecting strap 3 is fixedly connected between the outer sleeve 1 and the cover 2. The inner filter tube 4 is set on the protrusion inside the outer sleeve 1. A filter screen mechanism is provided on the bottom wall of the inner filter tube 4.
[0035] Furthermore, the barb 6 is fixedly connected to the top wall of the cover 2, and the first groove 10 is opened at the lower edge of the cover 2.
[0036] Furthermore, the two spring clips 7 are respectively fixedly connected to the inner walls on both sides of the first groove 10, and the two spring clips 7 are integrally formed with positioning grooves 8.
[0037] Furthermore, both spring cards 7 are integrally formed with arc surfaces 9, and the cotton swab stick 12 is set between the two positioning grooves 8.
[0038] Furthermore, the fiber fixing sleeve 13 is fixedly attached to one end of the cotton swab stick 12, and the fiber fixing sleeve 13 is starfruit-shaped.
[0039] Furthermore, the cotton fiber 14 is fitted onto the fiber fixing sleeve 13, the oblique conical surface 11 is opened at the opening of the outer sleeve 1, and the second groove 15 is provided with an integrally formed conical protrusion that matches the oblique conical surface 11.
[0040] Furthermore, a second groove 15 is provided on the conical protrusion, and a sealing ring 5 is placed in the second groove 15. The cotton fiber 14 is a soluble fiber that can be completely dissolved in the high-temperature pyrolysis liquid.
[0041] Furthermore, the fiber fixing sleeve 13 allows the cotton fibers 14 to be evenly wrapped around the front three-quarters of the surface of the fiber fixing sleeve 13. Scissors can be quickly inserted into the pre-reserved gap at the rear one-quarters to cut the attached cotton fibers 14. The soluble fibers can be completely dissolved in the high-temperature lysis solution. The cotton fibers 14 are coated with nano-silver particles, which play an antibacterial role. This avoids the problem that in the traditional method, after wiping biological samples with cotton swabs and putting them into evidence packaging bags, the introduction of bacteria and other microorganisms from the environment during the wiping process can easily cause the biological samples to be decomposed by microorganisms, resulting in a decrease in DNA content.
[0042] Furthermore, during collection, first hold the cotton swab 12 by one end and locate the microbial culture dish or microbial aggregation site to be collected. Then, align the cotton fibers 14 of the cotton swab 12 with the area to be swabbed and manually move the cotton swab 12 so that the cotton fibers 14 swab the microorganisms and attach them to the outer surface of the cotton fibers 14. After swabbing, manually rotate the cotton swab 12 to check if it is intact.
[0043] Furthermore, by setting the fiber fixing sleeve 13 to be made of silicone, it is possible for the fiber fixing sleeve 13 to better fit the surface of the object when the cotton swab 12 is used to wipe biological samples. This avoids the problem of low sampling efficiency caused by the cotton swab being too hard and having too small a contact area with the object in the traditional method.
[0044] Furthermore, after collection, first use cotton swab 12 to collect a trace amount of attached biological sample. Then, remove the cannula assembly, hold it with one hand, push open the cap 2 with your thumb, insert the cotton swab tip into the inner filter tube 4, press down the cap 2 so that the barb 6 is inserted into the gap at the rear 1 / 4 of the cotton swab tip, then press down the tube cap and pull the cotton swab 12 backward. At this time, the barb 6 will hook the cotton fibers 14 attached to the fiber fixing sleeve 13, so that all the cotton fibers 14 are transferred into the inner filter tube 4. Then close the cap 2. By wiping the biological sample during operation, the fibers... The fiber fixing sleeve 13 is inserted into the inclined conical surface 11. At this time, the arc surface 9 undergoes elastic deformation, and the cotton swab stick 12 is fixed between the two positioning grooves 8. Then, the cover 2 is loosely placed over the outer tube 1, and the cotton swab stick 12 can be pulled out backward. At this time, the barb 6 will be inserted into the gap of the fiber fixing sleeve 13, so that the inner filter tube 4 can be completely collected in the inner filter tube 4, thereby improving the efficiency of biological sample extraction and avoiding the problem that the cotton swab head and stick are not easy to separate when laboratory testers cut off the biological sample attached to the cotton swab head.
[0045] Furthermore, the outer sleeve 1 and inner filter tube 4 ensure that the cotton fibers 14 containing biological samples are well preserved, preventing the biological samples that are loosely attached to the surface of the swab from falling off during movement, transportation, and handover after the swabs are placed in the evidence packaging bag.
[0046] Furthermore, by setting the cotton fiber 14 to be a soluble fiber, it can be completely dissolved in the high-temperature lysis solution. This avoids the problem in the traditional method where, during the DNA extraction stage, the cotton fiber is insoluble in the lysis solution, and the testing process requires the removal of the cotton fiber through a "carrier removal" step. During lysis, the cotton fiber will carry some detached cells, resulting in DNA loss in the "carrier removal" step (separation and removal of cotton fiber), thus affecting the progress of the case.
[0047] Structural Description:
[0048] Outer tube 1: This is the main container of the entire tube assembly, used to house the internal components and protect biological samples from external contamination. Its opening is designed with a sloping conical surface 11 to facilitate the insertion and positioning of the fiber fixing sleeve 13.
[0049] Cover 2: It is threaded onto the outer tube 1 to close the opening of the outer tube 1. A barb 6 is fixedly connected to its top wall, and a first groove 10 is opened at the lower edge for installing a spring clip 7.
[0050] Connecting strap 3: It is fixedly connected between the outer sleeve 1 and the cover 2, and may be used to suspend or carry the entire sleeve assembly.
[0051] Inner filter tube 4: Located on the protrusion inside the outer tube 1, the inner filter tube 4 is used to collect and retain biological samples attached to the cotton fiber 14.
[0052] The barb 6 is fixedly connected to the top wall of the cover 2. When the cover 2 is partially closed, the barb 6 can be inserted into the gap of the fiber fixing sleeve 13 to prevent it from falling off.
[0053] Spring clips 7: Two spring clips 7 are fixedly connected to the inner walls of the two sides of the first groove 10 respectively, and are used to fix the cotton swab stick 12. They are integrally formed with positioning grooves 8 and arc surfaces 9.
[0054] Positioning groove 8: integrally formed on spring card 7, used to accommodate and fix cotton swab 12.
[0055] Arc surface 9: integrally formed on spring card 7. When fiber fixing sleeve 13 is inserted from the inclined conical surface 11, arc surface 9 will undergo elastic deformation, which facilitates the insertion of fiber fixing sleeve 13.
[0056] Cotton swab 12: Set between two positioning grooves 8 to support the fiber fixing sleeve 13. When in use, the cotton swab 12 can be pulled out backward to facilitate the insertion of the barb 6 into the gap of the fiber fixing sleeve 13.
[0057] 11: An oblique conical surface is formed at the opening of the outer sleeve 1 and is adapted to the conical surface on the conical protrusion. It is used to guide the insertion and positioning of the fiber fixing sleeve 13.
[0058] Fiber fixing sleeve 13: The fixing sleeve is attached to one end of the cotton swab stick. It is starfruit-shaped and made of silicone. It is easy to fit the surface of the item when taking samples, and the cotton fiber 14 can be evenly wrapped around four-thirds of its front end surface.
[0059] Cotton fiber 14: It is fitted onto the fiber fixing sleeve 13 and is used to collect and preserve biological samples. It is a soluble fiber that can be completely dissolved in the high-temperature lysis solution, avoiding the problem of cotton fiber being difficult to remove in the traditional method. At the same time, nano silver particles are sprayed on the cotton fiber 14, which play an antibacterial role.
[0060] Second groove 15: It is formed on the conical boss to accommodate the sealing ring 5.
[0061] Sealing ring 5: Set in the second groove 15 (or possibly directly installed at the junction of the outer tube 1 and the cover 2) to ensure the seal between the outer tube 1 and the cover 2.
[0062] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A kit for collecting and preserving trace biological evidence, characterized in that: The device includes an outer tube (1), on which a sleeve assembly is provided. The sleeve assembly is used to collect and preserve cotton fibers with attached biological samples. The sleeve assembly includes an inner filter tube (4), a barb (6), a spring clip (7), a first groove (10), an oblique cone (11), a cotton swab (12), a fiber fixing sleeve (13), cotton fibers (14), and a sealing ring (5). The cap (2) in the sleeve assembly is threaded onto the outer tube (1). The bottom of the outer tube (1) stores a desiccant. There is a connecting strip (3) between the outer tube (1) and the cap (2). The inner filter tube (4) is set on a protrusion inside the outer tube (1). A filter screen mechanism is provided on the bottom wall of the inner filter tube (4).
2. The trace biological evidence collection and preservation kit according to claim 1, characterized in that: The barb (6) is fixedly connected to the top wall of the cover (2), and the first groove (10) is opened at the lower edge of the cover (2).
3. The trace biological evidence collection and preservation kit according to claim 2, characterized in that: The two spring clips (7) are fixedly connected to the inner walls of the two sides of the first groove (10), and the two spring clips (7) are integrally formed with positioning grooves (8).
4. The trace biological evidence collection and preservation kit according to claim 3, characterized in that: Both spring cards (7) have an integrally formed arc surface (9), and the cotton swab stick (12) is set between two positioning grooves (8).
5. The trace biological evidence collection and preservation kit according to claim 4, characterized in that: The fiber fixing sleeve (13) is fixedly attached to one end of the cotton swab stick (12), and the fiber fixing sleeve (13) is starfruit shaped.
6. The trace biological evidence collection and preservation kit according to claim 5, characterized in that: The cotton fiber (14) is sleeved on the fiber fixing sleeve (13), the oblique cone surface (11) is opened at the opening of the outer sleeve (1), and the second groove (15) is provided with an integrally formed cone protrusion that matches the oblique cone surface (11).
7. The trace biological evidence collection and preservation kit according to claim 6, characterized in that: The conical protrusion has a second groove (15), and the sealing ring (5) is set in the second groove (15). The cotton fiber (14) is a soluble fiber that can be completely dissolved in the high-temperature pyrolysis liquid.