A cold stress experiment temperature control box
By setting up an air-sealing mechanism and airtight components in the temperature control chamber for cold stress experiments, the problem of low-temperature air escaping during animal replacement was solved, achieving effective retention of low-temperature gas, reducing energy consumption, shortening experimental preparation time, and improving experimental efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
- Filing Date
- 2025-07-30
- Publication Date
- 2026-06-02
AI Technical Summary
When changing experimental animals, the low-temperature air in the existing cold stress experimental temperature control chamber is prone to escape, which leads to increased energy consumption and extended experimental preparation time.
A temperature control chamber for cold stress experiments, including an air-sealing mechanism and a gas-tight assembly, was designed. By retaining low-temperature air when changing test objects, the loss of cold air is reduced. A ring-sealing assembly and an end-moving assembly are used to seal the channel groove. Combined with the movement of the inner sealing plate, the orderly flow and sealing of low-temperature gas in the chamber are ensured.
It effectively reduced the loss of cold air caused by changing test objects, lowered energy consumption, shortened experimental preparation time, and improved experimental efficiency and energy efficiency of equipment operation.
Smart Images

Figure CN224317949U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of temperature control boxes, specifically a temperature control box for cold stress experiments. Background Technology
[0002] In many fields such as biomedicine, animal husbandry and veterinary medicine, and materials science, cold stress experiments are an important means of studying the changes in the physiological, biochemical, and physical properties of organisms and materials under low-temperature environments. For example, in animal husbandry and veterinary medicine, cold stress experiments can be used to explore the physiological responses and adaptation mechanisms of animals in cold environments, providing a theoretical basis for optimizing animal husbandry environments. In materials science, cold stress experiments can be used to evaluate the performance stability of materials under low-temperature conditions, ensuring the reliability of materials in practical low-temperature applications.
[0003] In cold stress experiments, it is common to subject different groups of experimental animals to cold stress treatment. The purpose is to compare the effects of different treatment conditions (such as different temperature gradients, different drug interventions, etc.) on the animals' physiology, behavior, or metabolism. However, existing cold stress experimental temperature control chambers have certain shortcomings when changing experimental animals. When the experimenter opens the chamber to change animals, the low-temperature air inside the chamber will escape rapidly, causing the temperature inside the chamber to rise sharply. In order to restore the set low-temperature environment, the control system needs to restart the cooling process, which not only prolongs the experimental preparation time but also increases energy consumption to a certain extent. Therefore, a cold stress experimental temperature control chamber is proposed. Utility Model Content
[0004] The purpose of this invention is to provide a temperature control box for cold stress experiments, which has the advantages of retaining low-temperature air and improving experimental efficiency, and solves the problem of low-temperature gas escaping when changing test objects, making it difficult to reduce energy consumption.
[0005] To achieve the above objectives, the present invention provides the following technical solution: a cold stress experimental temperature control box, including a main box and a temperature control system installed on it, wherein an auxiliary box for experimental animals to enter and exit the main box is fixedly connected to one side of the main box, the auxiliary box and the main box are connected by gas, and the main box is provided with an air-sealing mechanism for opening and closing the connection between the main box and the auxiliary box and retaining the low-temperature gas in the main box.
[0006] The air-tightening mechanism includes a side plate fixedly connected to the main box, an outer fixed plate on the side of the side plate facing the auxiliary box, the outer fixed plate rotating on the auxiliary box on a fixed axis, and channel slots for experimental animals to pass through on both the side plate and the outer fixed plate. The side plate is provided with multiple sets of triangular seals, and the side plate is provided with a ring sealing assembly that drives the multiple sets of triangular seals to deflect synchronously to seal or open the channel slots.
[0007] The main housing is provided with an inner sealing plate, and the outer peripheral surface of the inner sealing plate is in sliding contact with the inner wall of the main housing. A transverse shaft is fixedly rotated on the main housing, and a set of rings in the same direction are fixedly sleeved on the horizontal sides of the main housing respectively. The main housing is provided with an end-moving component that drives the transverse shaft to move freely in the horizontal direction and rotate freely at the final position.
[0008] Preferably, the ring sealing assembly includes equidistant square grooves formed on the side plate for sliding connection of multiple sets of triangular sealing seats, and positioning pins are fixedly connected to the side of the multiple sets of triangular sealing seats facing the outer fixed plate. Positioning grooves for sliding connection of positioning pins are formed on the outer fixed plate.
[0009] The triangular sealing seat slides in contact with the opposing surfaces of the side plate and the outer fixed plate.
[0010] Preferably, the transverse shaft includes an integrally formed spline portion, a co-rotating disk on the main housing for the spline portion to slide through, a drive gear is slidably sleeved on the spline portion, a clamp is fixedly connected to the main housing, and the drive gear is in the clamp and slides in contact with the side wall of the clamp.
[0011] The driving gear is meshed with a driven gear ring, which is fixedly sleeved on the outer fixed plate.
[0012] Preferably, the end-moving assembly includes a base cylinder fixedly connected to the main housing, an end-direction column coaxially arranged on the base cylinder, the end-direction column and the transverse axis being fixed coaxially, and a circular hole for the end-direction column to slide through the base cylinder.
[0013] The end post includes an integrally formed shaft protrusion. The base cylinder has a left-side annular groove and a right-side annular groove for sliding connection of the shaft protrusion. The base cylinder also has a transverse limiting groove for horizontal sliding of the shaft protrusion. The two ends of the transverse limiting groove are respectively connected to the left-side annular groove and the right-side annular groove.
[0014] Preferably, the main housing is provided with an L-shaped sliding plate that can move freely in the horizontal direction, and an electric push rod for driving the L-shaped sliding plate is fixedly connected to the main housing. An adjustment pin is fixedly connected to the side of the L-shaped sliding plate facing the end column, and a spiral adjustment groove is provided on the end column for the adjustment pin to slide.
[0015] Preferably, the base cylinder has a rectangular through groove corresponding to the horizontal movement direction of the adjusting pin.
[0016] Preferably, the inner sealing plate is provided with multiple sets of airtight components for the circulation of low-temperature air inside the main box. The airtight components include a conical cavity groove opened on the inner sealing plate, and the middle part of the conical cavity groove includes an integrally formed narrow opening. The narrow opening is provided with a blocking ball that slides in contact with its inner wall.
[0017] A reset spring is fixedly connected to the stop ball. The end of the reset spring away from the stop ball is fixedly connected to the main housing. The main housing has a through groove corresponding to the conical cavity groove position, which communicates with the gas.
[0018] Compared with the prior art, the beneficial effects of this utility model are as follows:
[0019] 1. By setting up an air-sealing mechanism, this utility model can effectively retain most of the low-temperature air in the main chamber when changing the test object, thereby greatly reducing the loss of cold air caused by changing the test object, thus significantly reducing the energy consumption caused by recooling, shortening the experimental preparation time, improving experimental efficiency, reducing the operating cost of the equipment, and making the cold stress experiment more energy-efficient and efficient.
[0020] 2. By setting up an airtight component, this utility model allows low-temperature air to circulate orderly in the space on both sides of the inner sealing plate. When the position of the inner sealing plate remains unchanged, it can prevent the low-temperature air from escaping, thereby greatly reducing the loss of cold air caused by changing the test object. Attached Figure Description
[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0022] Figure 2 This is a schematic diagram of the component containing the outer fixing plate of this utility model;
[0023] Figure 3 This utility model Figure 2 Enlarged view of point A in the middle;
[0024] Figure 4 This is a schematic diagram of the component containing the triangular sealing seat of this utility model;
[0025] Figure 5 This is a schematic diagram of the component containing the transverse axis of this utility model;
[0026] Figure 6 This utility model Figure 5 Enlarged view at point B in the middle;
[0027] Figure 7 This is a schematic diagram of the component containing the base cylinder of this utility model;
[0028] Figure 8 This utility model Figure 7 Enlarged view at point C;
[0029] Figure 9 This is a schematic diagram of the component containing the adjusting pin of this utility model.
[0030] In the diagram: 1. Main housing; 2. Auxiliary housing; 3. Inner sealing plate; 4. Side positioning plate; 5. Triangular sealing seat; 6. Outer fixed plate; 7. Positioning pin; 8. Positioning groove; 9. Driven gear ring; 10. Driving gear; 11. Clamp; 12. Transverse shaft; 121. Spline section; 13. Co-directional ring; 14. Conical cavity groove; 15. Stop ball; 16. Return spring; 17. Base cylinder; 18. End column; 181. Shaft protrusion; 19. Left-direction ring groove; 20. Right-direction ring groove; 21. Transverse limiting groove; 22. L-shaped shift plate; 23. Adjusting pin; 24. Spiral adjusting groove; 25. Co-directional plate. Detailed Implementation
[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0032] Please see Figures 1 to 9 This utility model provides a technical solution: a cold stress experimental temperature control box includes a main box 1 and a temperature control system installed on it. An auxiliary box 2 for experimental animals to enter and exit the main box 1 is fixedly connected to one side of the main box 1. The auxiliary box 2 and the main box 1 are connected by gas. The main box 1 is provided with an air-sealing mechanism that opens and closes the connection position between the main box 1 and the auxiliary box 2 and retains the low temperature gas in the main box 1.
[0033] The air-tightening mechanism includes a side plate 4 fixedly connected to the main box 1. An outer fixed plate 6 is provided on the side of the side plate 4 facing the auxiliary box 2. The outer fixed plate 6 rotates on the auxiliary box 2 on a fixed axis. Both the side plate 4 and the outer fixed plate 6 are provided with channel slots for experimental animals to pass through. The side plate 4 is provided with multiple sets of triangular sealing seats 5, and the side plate 4 is provided with a ring sealing assembly that drives the multiple sets of triangular sealing seats 5 to deflect synchronously to seal or open the channel slots.
[0034] The main housing 1 is provided with an inner sealing plate 3. The outer peripheral surface of the inner sealing plate 3 is in sliding contact with the inner wall of the main housing 1. A transverse shaft 12 is fixedly rotated on the main housing 1. A set of rings 13 in the same direction are fixedly sleeved on the horizontal sides of the main housing 1 respectively. The main housing 1 is provided with an end-moving component that drives the transverse shaft 12 to move freely in the horizontal direction and rotate freely at the final position.
[0035] The inner sealing plate 3 is equipped with multiple sets of airtight components for the circulation of low-temperature air inside the main housing 1.
[0036] like Figure 1 , Figure 2 and Figure 5As shown, when changing the test animal in the main box 1, the transverse shaft 12 is driven to move freely in the horizontal direction by the end-moving component. Two sets of unidirectional rings 13 are fixedly sleeved on the transverse shaft 12, and the inner sealing plate 3 is located between the two sets of unidirectional rings 13. Therefore, when the transverse shaft 12 moves in the horizontal direction, the inner sealing plate 3 located therein can be driven to move synchronously in the horizontal direction by the two sets of unidirectional rings 13, thereby causing the inner sealing plate 3 to move in the main box 1.
[0037] Meanwhile, the inner sealing plate 3 is equipped with a gas-tight assembly for gas flow. Driven by the gas-tight assembly, when the inner sealing plate 3 moves to the side of the attached box 2, the low-temperature air in the right space of the inner sealing plate 3 enters the left space of the inner sealing plate 3 through the gas-tight assembly as the inner sealing plate 3 moves horizontally. At the same time, driven by the gas-tight assembly, when the position of the inner sealing plate 3 remains unchanged, the low-temperature air in the left space of the inner sealing plate 3 is difficult to escape to the left space, so that most of the low-temperature air in the main box 1 can be retained when changing experimental animals.
[0038] It should be noted that when the inner sealing plate 3 moves toward the attachment box 2, it can drive away the test animals in the main box 1, thereby restricting the activity space of the test animals. When the inner sealing plate 3 is in the final position near the attachment box 2, the transverse axis 12 can rotate in the vertical direction, thereby causing the ring sealing assembly to operate. The ring sealing assembly causes multiple sets of triangular sealing seats 5 to deflect to drive the channel groove to be unobstructed. Subsequently, through manual guidance, the test animals are prompted to enter the attachment box 2.
[0039] Meanwhile, the experimental animal to be replaced is placed into the attachment box 2 through the freely opening hatch on one side of the attachment box 2. Subsequently, the inner sealing plate 3 is driven away from the attachment box 2 by the end-moving component until the inner sealing plate 3 is at the end position away from the attachment box 2. Then, the experimental animal is guided and driven into the main box 1 through the channel groove. During this process, the low temperature air in the space on the left side of the inner sealing plate 3 enters the space on the right side of the inner sealing plate 3 through the airtight component. Subsequently, the ring sealing component drives multiple sets of triangular seals 5 to close the channel groove, so that the inside of the main box 1 is a sealed space. The temperature control system promotes the temperature to reach the set temperature to complete the cold stress experiment process of the experimental animal.
[0040] In one preferred embodiment, the ring sealing assembly includes equidistant square grooves formed on the side plate 4 for sliding connection of multiple sets of triangular sealing seats 5. Each set of triangular sealing seats 5 is fixedly connected to a positioning pin 7 on the side facing the outer fixed plate 6. The outer fixed plate 6 is provided with a positioning groove 8 for sliding connection of the positioning pin 7.
[0041] The triangular sealing seat 5 slides in contact with the opposite surfaces of the side plate 4 and the outer fixed plate 6.
[0042] The transverse shaft 12 includes an integrally formed spline portion 121. A coaxial disk 25 is fixedly rotatable on the main housing 1, through which the spline portion 121 slides. A drive gear 10 is slidably sleeved on the spline portion 121. A clamp 11 is fixedly connected to the main housing 1. The drive gear 10 is located in the clamp 11 and slides in contact with the side wall of the clamp 11. The drive gear 10 is meshed with a driven gear ring 9, which is fixedly sleeved on the outer fixed disk 6.
[0043] like Figure 1 - Figure 4 As shown, when the transverse shaft 12 rotates in the vertical direction, it can drive the drive gear 10 sleeved on it to rotate synchronously through the spline part 121. The drive gear 10 is meshed with the driven gear ring 9, and the driven gear ring 9 is fixedly sleeved on the outer fixed plate 6. Thus, the rotation process of the transverse shaft 12 drives the outer fixed plate 6 to rotate synchronously in the vertical direction.
[0044] At the same time, when the outer fixed plate 6 rotates, it can drive multiple sets of positioning pins 7 and triangular seals 5 to deflect synchronously through multiple sets of positioning grooves 8, thereby preventing multiple sets of triangular seals 5 from closing the channel groove. In actual use, this facilitates the replacement of the test object in the auxiliary box 2, so that the replaced test object can enter the main box 1 through the channel groove.
[0045] Based on the ring sealing assembly embodiment, the end shifting assembly includes a base cylinder 17 fixedly connected to the main housing 1, an end post 18 coaxially arranged on the base cylinder 17, the end post 18 and the transverse shaft 12 are coaxially fixed, and a circular hole is opened on the base cylinder 17 for the end post 18 to slide through.
[0046] The end post 18 includes an integrally formed shaft protrusion 181. The base cylinder 17 is provided with a left-facing annular groove 19 and a right-facing annular groove 20 for the shaft protrusion 181 to slide. The base cylinder 17 is also provided with a transverse limiting groove 21 for the shaft protrusion 181 to slide horizontally. The two ends of the transverse limiting groove 21 are respectively connected to the left-facing annular groove 19 and the right-facing annular groove 20.
[0047] The main housing 1 is provided with an L-shaped sliding plate 22 that moves freely in the horizontal direction, and an electric push rod for driving the L-shaped sliding plate 22 is fixedly connected to the main housing 1. An adjusting pin 23 is fixedly connected to the side of the L-shaped sliding plate 22 facing the end column 18. A spiral adjusting groove 24 for the adjusting pin 23 to slide is opened on the end column 18. A rectangular through groove is opened on the base cylinder 17 corresponding to the horizontal movement direction of the adjusting pin 23.
[0048] like Figure 1 , Figure 6 , Figure 7 , Figure 8 and Figure 9As shown, in the initial state, the adjusting pin 23 is located at the end of the spiral adjusting groove 24 away from the base cylinder 17, and at this time, the shaft protrusion 181 is at the junction of the left annular groove 19 and the transverse limiting groove 21. When the L-shaped moving plate 22 and the adjusting pin 23 on it are driven to move towards the main housing 1 by the electric push rod, due to the resistance between the adjusting pin 23 and the spiral adjusting groove 24, the shaft protrusion 181 first slides on the transverse limiting groove 21 until the shaft protrusion 181 moves horizontally to the junction of the right annular groove 20 and the transverse limiting groove 21. During this process, the end column 18 and the transverse shaft 12 can move in the horizontal direction, and through the two sets of same-direction rings 13, the inner sealing plate 3 moves in the main housing 1 to the end position near the attachment box 2.
[0049] At the same time, when the shaft protrusion 181 moves to the position where the transverse limiting groove 21 and the right-hand annular groove 20 meet, under the restriction of the right-hand annular groove 20, the shaft protrusion 181 can no longer follow the horizontal movement of the L-shaped moving plate 22. Subsequently, when the L-shaped moving plate 22 moves closer to the main housing 1, it can cause the adjusting pin 23 to slide on the spiral adjusting groove 24, thereby causing the end column 18 and the transverse shaft 12 to rotate in the vertical direction, thereby driving the ring seal assembly to operate.
[0050] It should be noted that in actual use, when the shaft protrusion 181 rotates clockwise on the right-hand annular groove 20, at the end of its clockwise rotation, the shaft protrusion 181 is at the junction of the right-hand annular groove 20 and the transverse limiting groove 21. Then, when the electric actuator drives the L-shaped moving plate 22 and the adjusting pin 23 to move horizontally away from the main housing 1, the shaft protrusion 181 first slides on the transverse limiting groove 21 until it moves to the junction of the transverse limiting groove 21 and the left-hand annular groove 19. At this time, the inner... As the sealing plate 3 moves away from the auxiliary box 2 within the main box 1, and as the shaft protrusion 181 aligns with the left-facing annular groove 19, it continues to move away from the main box 1 along with the L-shaped shift plate 22. This drives the adjusting pin 23 to slide on the spiral adjusting groove 24, thereby causing the end column 18 and the transverse shaft 12 to reverse, so that the annular sealing assembly can seal the channel groove again. This ensures that the main box 1 can seal the inner wall space of the main box 1 when conducting cold stress experiments on the experimental object, thus preventing the escape of low-temperature gas.
[0051] Based on the end-shifting assembly embodiment, the airtight assembly includes a conical cavity 14 formed on the inner sealing plate 3. The middle part of the conical cavity 14 includes an integrally formed narrow opening, and the narrow opening is provided with a stop ball 15 that slides in contact with its inner wall.
[0052] A reset spring 16 is fixedly connected to the stop ball 15. The end of the reset spring 16 away from the stop ball 15 is fixedly connected to the main housing 1. The main housing 1 has a through groove corresponding to the conical cavity 14, which is connected to the gas.
[0053] like Figure 1 , Figure 5 and Figure 6 As shown, when the inner sealing plate 3 moves horizontally within the main housing 1 following the transverse axis 12, it can compress the low-temperature gas within the main housing 1, thereby causing the low-temperature gas in the main housing 1 to flow within the conical cavity 14. The flow direction of the low-temperature gas is determined by the moving direction of the inner sealing plate 3. When the gas flows within the conical cavity 14, it can push the stop ball 15 and cause the return spring 16 to deform, driving the stop ball 15 to disengage from the narrow opening. This allows the gas in the horizontal spaces on both sides of the inner sealing plate 3 to flow freely as the inner sealing plate 3 moves.
[0054] Meanwhile, when the inner sealing plate 3 is in the terminal position and no longer moves, under the action of the elastic potential energy of the return spring 16, the blocking ball 15 is in the narrow opening position and can block the narrow opening, thereby preventing the low temperature air in the space on the left side of the inner sealing plate 3 from escaping into the auxiliary chamber 2, so that when the test object is changed, some of the low temperature gas in the main chamber 1 can be retained, thereby reducing the subsequent cooling process, shortening the subsequent test time, and reducing the energy consumption of the temperature control system to a certain extent.
[0055] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A temperature control chamber for cold stress experiments, comprising a main chamber (1) and a temperature control system installed thereon, characterized in that: The main box (1) is fixedly connected to one side of an auxiliary box (2) for experimental animals to enter and exit the main box (1). The auxiliary box (2) and the main box (1) are connected by gas. The main box (1) is provided with a gas-sealing mechanism that opens and closes the connection between the main box (1) and the auxiliary box (2) and retains the low-temperature gas inside the main box (1). The air-sealing mechanism includes a side plate (4) fixedly connected to the main box (1). The side plate (4) has an outer fixed plate (6) on the side facing the auxiliary box (2). The outer fixed plate (6) rotates on the auxiliary box (2) on a fixed axis. Both the side plate (4) and the outer fixed plate (6) have channel slots for experimental animals to pass through. The side plate (4) has multiple sets of triangular seals (5). The side plate (4) also has a ring sealing assembly that drives the multiple sets of triangular seals (5) to deflect synchronously to seal or open the channel slots. The main housing (1) is provided with an inner sealing plate (3), the outer circumferential surface of the inner sealing plate (3) is in sliding contact with the inner wall of the main housing (1), a transverse shaft (12) is fixedly rotated on the main housing (1), and a set of rings (13) in the same direction are fixedly sleeved on the horizontal sides of the main housing (1) respectively. The main housing (1) is provided with an end-moving component that drives the transverse shaft (12) to move freely in the horizontal direction and rotate freely at the final position.
2. The temperature control chamber for cold stress experiments according to claim 1, characterized in that: The ring sealing assembly includes an equidistant square groove on the side plate (4) for sliding connection of multiple sets of triangular sealing seats (5), and a positioning pin (7) is fixedly connected to the side of the multiple sets of triangular sealing seats (5) facing the outer fixed plate (6). The outer fixed plate (6) is provided with a positioning groove (8) for sliding connection of the positioning pin (7). The triangular sealing seat (5) slides in contact with the opposite surfaces of the side plate (4) and the outer fixed plate (6).
3. The temperature control chamber for cold stress experiments according to claim 2, characterized in that: The transverse shaft (12) includes an integrally formed spline part (121), and a co-rotating disk (25) is fixedly mounted on the main housing (1) for the spline part (121) to slide through. A drive gear (10) is slidably sleeved on the spline part (121), and a clamp (11) is fixedly connected to the main housing (1). The drive gear (10) is located in the clamp (11) and slides in contact with the side wall of the clamp (11). The driving gear (10) is meshed with a driven gear ring (9), which is fixedly sleeved on the outer fixed plate (6).
4. The temperature control chamber for cold stress experiments according to claim 3, characterized in that: The end-moving assembly includes a base cylinder (17) fixedly connected to the main housing (1), and an end column (18) coaxially arranged on the base cylinder (17). The end column (18) and the transverse shaft (12) are coaxially fixed together. A circular hole is opened on the base cylinder (17) for the end column (18) to slide through. The end post (18) includes an integrally formed shaft protrusion (181). The base cylinder (17) is provided with a left-facing annular groove (19) and a right-facing annular groove (20) for sliding connection of the shaft protrusion (181). The base cylinder (17) is also provided with a transverse limiting groove (21) for horizontal sliding of the shaft protrusion (181). The two ends of the transverse limiting groove (21) are respectively connected to the left-facing annular groove (19) and the right-facing annular groove (20).
5. A temperature control chamber for cold stress experiments according to claim 4, characterized in that: The main housing (1) is provided with an L-shaped sliding plate (22) that moves freely in the horizontal direction, and an electric push rod for driving the L-shaped sliding plate (22) is fixedly connected to the main housing (1). An adjusting pin (23) is fixedly connected to the side of the L-shaped sliding plate (22) facing the end column (18), and a spiral adjusting groove (24) for the adjusting pin (23) to slide is opened on the end column (18).
6. A temperature control chamber for cold stress experiments according to claim 5, characterized in that: The base cylinder (17) has a rectangular through groove in the horizontal movement direction corresponding to the adjustment pin (23).
7. A temperature control chamber for cold stress experiments according to claim 1, characterized in that: The inner sealing plate (3) is provided with multiple sets of airtight components for the circulation of low-temperature air inside the main box (1). The airtight components include a conical cavity (14) opened on the inner sealing plate (3). The middle part of the conical cavity (14) includes an integrally formed narrow opening. The narrow opening is provided with a stop ball (15) that slides in contact with its inner wall. A reset spring (16) is fixedly connected to the stop ball (15). The end of the reset spring (16) away from the stop ball (15) is fixedly connected to the main housing (1). The main housing (1) has a through groove that communicates with the gas at the position corresponding to the conical cavity groove (14).