Electrochemical test paper for creatinine

Abstract

The utility model relates to a kind of creatinine electrochemical detection test paper, belong to detection equipment technical field, especially creatinine electrochemical detection equipment technical field.The utility model is equipped with two groups of detection electrode with non-closed frame electrode structure and two independent blood tank channels opened by double-sided adhesive layer, the reaction of creatinine in blood sample and the reaction of creatine are independently carried out in two blood tanks, creatinine and creatine signals in blood sample can be detected simultaneously without mutual interference, so that the detection of creatinine is more accurate.In addition, the compact multilevel test paper structure design of the utility model not only reduces the volume of test paper, but also the design and layout of reasonable blood tank channel, escape gas port and detection electrode can facilitate the precise use of detector.

Description

Technical Field

[0001] This utility model relates to an electrochemical test strip for creatinine detection, belonging to the technical field of detection equipment, and particularly to the technical field of electrochemical detection equipment for creatinine. Background Technology

[0002] Creatinine electrochemical test strips are a detection tool for rapid and convenient detection of creatinine levels, mainly based on the principles of enzyme-catalyzed reaction and electrochemical detection.

[0003] Currently, existing creatinine electrochemical test strips have two detection areas set in a reaction blood tank. One detection area reacts with creatinine and creatine in the blood sample to obtain a signal A; the other detection area reacts with creatine in the blood sample to obtain a signal B. The obtained AB is the signal value of creatinine in the blood sample.

[0004] However, since creatinine and creatine react in the same blood bath, the detection electrodes will inevitably interfere with each other to some extent when detecting the two reaction signals, leading to inaccurate test results. Currently, there is no detection device in the existing technology that can avoid interference between the two reaction signals. Utility Model Content

[0005] To address the above problems, this utility model provides an electrochemical test strip for creatinine detection, comprising:

[0006] Substrate;

[0007] Two sets of detection electrodes, identical in shape and symmetrically distributed on the substrate, each set of detection electrodes includes a non-closed frame electrode structure, and a first enzyme layer and a second enzyme layer are respectively disposed on the frame electrode structure of the two sets of detection electrodes;

[0008] A double-sided adhesive layer has two independent blood groove channels, and the double-sided adhesive layer is bonded above the two detection electrodes and simultaneously covers at least a portion of the two detection electrodes;

[0009] A hydrophilic layer is bonded above the double-sided adhesive layer, and the hydrophilic layer has air vents.

[0010] Furthermore, the first enzyme layer includes creatine amide hydrolase solid, creatine kinase solid, and sarcosine oxidase solid, all of which are block-shaped and overlapped. The second enzyme layer includes creatine kinase solid and sarcosine oxidase solid, both of which are block-shaped and overlapped.

[0011] Furthermore, each set of detection electrodes includes a left detection electrode and a right detection electrode. The left and right detection electrodes are both C-shaped structures and are arranged opposite each other to form a frame-type electrode structure. There is a gap between the left and right detection electrodes and they are not connected to each other.

[0012] Furthermore, one end of the left detection electrode and the corresponding end of the right detection electrode are flush with each other and located at one end of the substrate, and the other end of the left detection electrode and the corresponding end of the right detection electrode are arranged in a cross configuration. The first enzyme layer or the second enzyme layer is disposed at the end where the left and right detection electrodes are arranged in a cross configuration.

[0013] Furthermore, the double-sided adhesive layer is a rectangular sheet structure with two blood groove channels at one end. The two blood groove channels are symmetrically arranged on both sides of one end of the double-sided adhesive layer. An isolation strip is provided between the two blood groove channels, and the two blood groove channels are not interconnected.

[0014] Furthermore, both blood groove channels are fan-shaped openings and are symmetrically arranged on both sides of the isolation strip, with the first enzyme layer and the second enzyme layer exposed in the two blood groove channels respectively.

[0015] Furthermore, the hydrophilic layer is a rectangular sheet structure and is attached above the double-sided adhesive layer, and the air escape hole is connected to both blood groove channels.

[0016] Furthermore, the vent is an elliptical vent.

[0017] The beneficial effects of this utility model are:

[0018] This invention utilizes two sets of detection electrodes with non-closed frame electrode structures, combined with two independent blood groove channels created by a double-sided adhesive layer. This allows the reactions of creatinine and creatine in the blood sample to occur independently in two separate blood grooves, enabling simultaneous detection of both creatinine and creatine signals without mutual interference, thus improving the accuracy of creatinine detection. Furthermore, the compact multi-layered test strip design of this invention not only reduces the size of the test strip but also facilitates precise use by the user through the rational design and layout of the blood groove channels, air vents, and detection electrodes. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure after assembly in one embodiment of the present invention;

[0020] Figure 2 This is an exploded view of the overall structure in one embodiment of the present invention;

[0021] Figure 3 This is another exploded view of the overall structure in one embodiment of the present invention;

[0022] In the figure: 1. Substrate; 2. Detection electrode; 3. Double-sided adhesive layer; 4. Hydrophilic layer; 5. First enzyme layer; 6. Second enzyme layer; 7. Exhaust pore; 31. Blood groove channel; 32. Isolation strip. Detailed Implementation

[0023] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0024] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. Specifically, the terms "first position" and "second position" refer to two different positions.

[0025] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections or detachable connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0026] like Figures 1-3 As shown, this utility model provides an electrochemical test strip for creatinine, comprising a substrate 1, two sets of detection motors 2, a double-sided adhesive layer 3, and a hydrophilic layer 4 stacked sequentially upwards.

[0027] The substrate 1 is a plate-like structure made of polyethylene terephthalate (PET), and two sets of detection electrodes 2 are installed on the upper surface of the substrate 1.

[0028] Two sets of detection electrodes 2 are identical in shape and symmetrically distributed on the upper surface of the substrate 1. Each set of detection electrodes 2 includes a non-closed frame-shaped electrode structure. Each set of detection electrodes 2 includes a left detection electrode and a right detection electrode. The left detection electrode and the right detection electrode are both C-shaped structures and are arranged opposite to each other to form a frame-shaped electrode structure. There is a gap between the left detection electrode and the right detection electrode and they are not connected to each other.

[0029] One end of the left detection electrode and the corresponding end of the right detection electrode are flush and located at one end of the substrate 1. Figure 1-3 The right end of the left detection electrode and the corresponding end of the right detection electrode are arranged in a cross configuration. Figure 1-3 The first enzyme layer 5 or the second enzyme layer 6 is disposed at one end of the intersection of the left and right detection electrodes (at the left end of the electrode), and the first enzyme layer 5 or the second enzyme layer 6 can simultaneously adhere to the intersection of the left and right detection electrodes.

[0030] The first enzyme layer 5 includes creatine amide hydrolase solid, creatine kinase solid, and sarcosine oxidase solid, all of which are block-shaped and overlapped. The second enzyme layer 6 includes creatine kinase solid and sarcosine oxidase solid, both of which are block-shaped and overlapped.

[0031] The double-sided adhesive layer 3 has two independent blood groove channels 31. The double-sided adhesive layer 3 is bonded above the two detection electrodes and simultaneously covers at least a portion of the frame-shaped electrode structure of the two detection electrodes 2. The double-sided adhesive layer 3 is a rectangular sheet structure with two blood groove channels 31 at one end. The two blood groove channels 31 are symmetrically arranged on both sides of one end of the double-sided adhesive layer 3. A separator strip 32 is provided between the two blood groove channels 31, and the two blood groove channels 31 are not interconnected. Both blood groove channels 31 are fan-shaped openings and symmetrically arranged on both sides of the separator strip 32. The first enzyme layer 5 and the second enzyme layer 6 are respectively exposed in the two blood groove channels 31.

[0032] A hydrophilic layer 4 is adhered above the double-sided adhesive layer 3, and the hydrophilic layer 4 has vent holes 7. The hydrophilic layer 4 has a rectangular sheet structure and is attached above the double-sided adhesive layer 3. The vent holes 7 are connected to both blood groove channels 31. The vent holes 7 are elliptical holes. The hydrophilic layer 4 is made of polyester film.

[0033] During testing, when the blood sample is brought close to the creatinine electrochemical test strip, the two blood groove channels 31 will automatically draw in the blood sample. The creatinine and creatine in the blood will react in the two blood grooves respectively. The detection electrode 2 can simultaneously detect the signal values ​​in the two blood groove channels 31 and calculate the signal value of creatinine.

[0034] Specifically, when the blood sample to be tested comes into contact with the sample application port of the creatinine electrochemical test paper, due to the hydrophilic effect of the hydrophilic layer 4 on the creatinine electrochemical test paper, the blood sample will be drawn into the creatinine electrochemical test paper along the hydrophilic layer 4. The blood sample will fill the blood groove channel 31 respectively, and different reactions will occur in the two blood groove channels 31 respectively.

[0035] For example, in the blood channel 31 where the first enzyme layer 5 is located, the creatinine in the blood sample undergoes the following reaction:

[0036]

[0037] Creatine in the blood sample undergoes the following reaction:

[0038]

[0039] Creatinine and creatine can both generate hydrogen peroxide through specific enzyme-catalyzed reactions. When the detection instrument applies a fixed voltage to the detection electrode 2, the hydrogen peroxide generated by the reaction of creatinine and creatine will be oxidized and reduced to release electrons and form an electric current. The detection instrument can obtain the concentration signal A of creatinine and creatine in the blood sample by detecting the current.

[0040] For example, in the blood channel 31 where the first enzyme layer 6 is located, since there is no creatinine amide hydrolase in this detection area, the creatinine in the blood sample does not react, and only creatine in the blood sample reacts as follows:

[0041]

[0042] Creatine is converted into hydrogen peroxide through a specific enzyme-catalyzed reaction. When the detection instrument applies a fixed voltage to the detection electrode 2, the hydrogen peroxide produced by the creatine reaction is oxidized and reduced to release electrons and form an electric current. The detection instrument can obtain the concentration signal B of creatine in the blood sample by detecting the current.

[0043] The concentration signals of creatinine and creatine in a blood sample are A and B, respectively. The concentration of creatinine in the blood sample can be obtained by converting the concentration signals of creatinine and creatine in the blood sample.

[0044] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims.

Claims

1. A creatinine electrochemical test strip, characterized in that, include: Substrate; Two sets of detection electrodes are identical in shape and symmetrically distributed on the substrate. Each set of detection electrodes includes a non-closed frame electrode structure. A first enzyme layer and a second enzyme layer are respectively disposed on the frame electrode structure of the two sets of detection electrodes. A double-sided adhesive layer has two independent blood groove channels, and the double-sided adhesive layer is bonded above the two detection electrodes and simultaneously covers at least a portion of the two detection electrodes; A hydrophilic layer is bonded above the double-sided adhesive layer, and the hydrophilic layer has air vents.

2. The creatinine electrochemical test strip according to claim 1, characterized in that, The first enzyme layer includes creatine amide hydrolase solid, creatine kinase solid, and sarcosine oxidase solid, all of which are block-shaped and overlapped. The second enzyme layer includes creatine kinase solid and sarcosine oxidase solid, both of which are block-shaped and overlapped.

3. The creatinine electrochemical test strip according to claim 2, characterized in that, Each set of detection electrodes includes a left detection electrode and a right detection electrode. The left and right detection electrodes are both C-shaped structures and are arranged opposite each other to form a frame-type electrode structure. There is a gap between the left and right detection electrodes and they are not connected to each other.

4. The creatinine electrochemical test strip according to claim 3, characterized in that, One end of the left detection electrode and the corresponding end of the right detection electrode are flush with each other and located at one end of the substrate. The other end of the left detection electrode and the corresponding end of the right detection electrode are arranged in an intersecting manner. The first enzyme layer or the second enzyme layer is disposed at the end where the left and right detection electrodes are arranged in an intersecting manner.

5. The creatinine electrochemical test strip according to claim 4, characterized in that, The double-sided adhesive layer is a rectangular sheet structure with two blood groove channels at one end. The two blood groove channels are symmetrically arranged on both sides of one end of the double-sided adhesive layer. An isolation strip is provided between the two blood groove channels, and the two blood groove channels are not interconnected.

6. The creatinine electrochemical test strip according to claim 5, characterized in that, Both blood groove channels are fan-shaped and symmetrically arranged on both sides of the isolation strip, with the first enzyme layer and the second enzyme layer exposed in the two blood groove channels respectively.

7. The creatinine electrochemical test strip according to claim 6, characterized in that, The hydrophilic layer is a rectangular sheet structure and is attached above the double-sided adhesive layer. The air escape hole is connected to both blood groove channels.

8. The creatinine electrochemical test strip according to claim 7, characterized in that, The vent is an elliptical hole.

Images