A sampling device for a bioreactor

Abstract

This utility model discloses a sampling device for a bioreactor, including a tank body with a tank cover. A heat dissipation cylinder is installed on the upper left side of the tank cover. A fan is installed on the upper part of the heat dissipation cylinder, and a Peltier is installed on the lower part of the heat dissipation cylinder. A first heat-conducting plate is installed on the hot end of the Peltier, and a second heat-conducting plate is installed on the cold end of the Peltier. A cooling water tank is installed on the right side of the heat dissipation cylinder, and a liquid sampling pipe is installed inside the cooling water tank. A heat-conducting pipe is installed inside the liquid sampling pipe, and a temperature sensor is installed on the inner wall of the cooling water tank. This sampling device for a bioreactor cools the water inside the cooling water tank by using the Peltier and the second heat-conducting plate, and cools the sampled liquid with the heat-conducting pipe. This allows the sampled liquid to be cooled in an ice bath or stored at a certain temperature to inhibit enzyme activity, freeze the metabolic process, and avoid metabolic activity affecting the accuracy of subsequent data detection.

Description

Technical Field

[0001] This utility model relates to the field of sampling device technology, specifically a sampling device for a bioreactor. Background Technology

[0002] A bioreactor is a reaction system that utilizes naturally occurring microorganisms or microorganisms with special degradation capabilities to inoculate a liquid or solid phase. The two most studied types of reactors are the "elevator reactor" and the "soil slurry reactor." The elevator reactor uses the flow of the water phase to provide appropriate nutrients, carbon sources, and oxygen, thereby achieving the purpose of degrading pollutants in the soil. During the operation of a bioreactor, samples need to be taken for testing to understand the reaction situation, and the sampling device is an essential piece of equipment for sampling in a bioreactor.

[0003] Chinese Patent Publication No. CN215953080U discloses a sampling device for a bioreactor, including a tank. A drive motor is fixedly installed on the top of the tank, and a rotating shaft is installed below the drive motor. A stirring blade is fixedly installed on the rotating shaft. A through-connected sampling tube is fixedly installed on the top left side of the tank, and the lower part of the sampling tube is inserted into the tank. A telescopic tube is fixedly installed at the lower end of the sampling tube, and a support ring is fixedly installed at the lower end of the telescopic tube. A fixing plate is fixedly installed on the left side of the support ring, and a threaded hole is opened on the top of the fixing plate. A positioning plate is fixedly installed on the top left side of the tank. A positioning hole is opened at the relative position of the positioning plate and the tank, and a push-pull rod is inserted into the positioning hole. This facilitates better sampling of liquid at different depths inside the tank through the telescopic tube, making the liquid detection structure more accurate.

[0004] Existing bioreactor sampling devices, when sampling, if the sample temperature remains at a high temperature inside the reactor (such as 37°C or higher), the metabolic activity of cells or microorganisms will continue, leading to changes in metabolite concentration and affecting subsequent detection results. To address this, we propose a new sampling device for bioreactors. Utility Model Content

[0005] The purpose of this invention is to provide a sampling device for a bioreactor, in order to solve the problem mentioned in the background art that when the sample temperature is maintained at a high temperature inside the reactor (such as 37°C or higher) after sampling, the metabolic activity of cells or microorganisms will continue, leading to changes in the concentration of metabolites, which will affect the subsequent detection results.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a sampling device for a bioreactor, comprising:

[0007] The tank has a lid on top, a heat sink on the upper left side of the lid, a fan inside the heat sink, a Peltier inside the heat sink, a first heat-conducting fin on the hot end of the Peltier, a second heat-conducting fin on the cold end of the Peltier, a cooling water tank on the right side of the heat sink, a liquid extraction pipe inside the cooling water tank, a heat-conducting pipe inside the liquid extraction pipe, a temperature sensor on the inner wall of the cooling water tank, an electric push rod on the right side of the cooling water tank, a first valve above the liquid extraction pipe, a lift pipe below the liquid extraction pipe, and a sealing ring on the lower end of the outer surface of the liquid extraction pipe.

[0008] Preferably, the heat pipe extends through the interior of the cooling water tank.

[0009] Preferably, the second heat-conducting sheet passes through the inner walls of the heat dissipation cylinder and the cooling water tank in sequence.

[0010] Preferably, the output end of the electric push rod is fixedly connected to the lifting tube, and the sealing ring is in contact with the inner wall of the lifting tube, and the lifting tube moves up and down along the liquid collection tube by means of the electric push rod.

[0011] Preferably, the can lid is further provided with:

[0012] A stirring motor is installed above the center of the tank cover. A drive shaft is installed at the output end of the stirring motor, and a stirring rod is installed on the outer surface of the drive shaft. The stirring rod rotates inside the tank through the stirring motor and the drive shaft.

[0013] Preferably, the can lid is further provided with:

[0014] An air cylinder is installed on the upper right side of the canister cap. A piston is movably installed inside the air cylinder. A first one-way valve is installed on the left side of the air cylinder. A second one-way valve is installed at the bottom of the air cylinder. An air bag is installed below the air cylinder. A second valve is provided on the right side of the second one-way valve. The second valve is connected to the air bag.

[0015] Compared with the prior art, the present invention provides a sampling device for a bioreactor, which has the following beneficial effects: the sampling device for the bioreactor cools the water inside the cooling water tank by setting a Peltier and a second heat-conducting plate, and cools the sampled liquid with a heat-conducting pipe, so that the sampled liquid is cooled in an ice bath or stored at ℃ to inhibit enzyme activity, freeze the metabolic process, and avoid metabolic activities affecting the accuracy of subsequent data detection. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the connection structure between the lifting pipe and the liquid extraction pipe of this utility model;

[0018] Figure 3 This is a schematic diagram of the internal structure of the cooling water tank of this utility model.

[0019] In the diagram: 1. Tank body; 2. Lifting pipe; 3. Tank lid; 4. Heat sink; 5. Cooling water tank; 6. Liquid extraction pipe; 7. First valve; 8. Electric push rod; 9. Stirring motor; 10. Air cylinder; 11. Piston; 12. First one-way valve; 13. Second valve; 14. Second one-way valve; 15. Airbag; 16. Drive shaft; 17. Stirring rod; 18. Sealing ring; 19. Fan; 20. First heat-conducting plate; 21. Peltier; 22. Second heat-conducting plate; 23. Heat-conducting pipe; 24. Temperature sensor. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figure 1-3A sampling device for a bioreactor includes a tank 1, a tank cover 3 mounted on top of the tank 1, a heat sink 4 mounted on the upper left side above the tank cover 3, a fan 19 mounted on the upper part of the heat sink 4, a Peltier 21 mounted on the lower part of the heat sink 4, a first heat-conducting plate 20 mounted on the hot end of the Peltier 21, a second heat-conducting plate 22 mounted on the cold end of the Peltier 21, and a cooling water tank 5 mounted on the right side of the heat sink 4; the second heat-conducting plate 22 passes through the inner walls of the heat sink 4 and the cooling water tank 5 sequentially, cooling... A liquid extraction pipe 6 is installed inside the water tank 5, and a heat-conducting pipe 23 is installed inside the liquid extraction pipe 6. The heat-conducting pipe 23 runs through the interior of the cooling water tank 5. A temperature sensor 24 is installed on the inner wall of the cooling water tank 5. An electric push rod 8 is installed on the right side of the cooling water tank 5. A first valve 7 is installed above the liquid extraction pipe 6, and a lifting pipe 2 is installed below the liquid extraction pipe 6. A sealing ring 18 is installed at the lower end of the outer surface of the liquid extraction pipe 6. The output end of the electric push rod 8 is fixedly connected to the lifting pipe 2, and the sealing ring 18 is in contact with the inner wall of the lifting pipe 2. The lifting tube 2 moves up and down along the liquid collection tube 6 via an electric push rod 8; the water inside the cooling water tank 5 is cooled by setting a Peltier 21 and a second heat-conducting plate 22, and the sampled liquid is cooled by the heat-conducting pipe 23, so that the sampled liquid is cooled in an ice bath or stored at 4°C to inhibit enzyme activity, freeze the metabolic process, and avoid the metabolic activity from affecting the accuracy of subsequent data detection; the stirring motor 9 is installed in the middle of the upper part of the tank cover 3, and the output end of the stirring motor 9 is equipped with a drive shaft 16. The outer surface of the drive shaft 16 is equipped with a stirring rod 17, and the stirring rod 17 rotates inside the tank 1 via the stirring motor 9 and the drive shaft 16; the air cylinder 10 is installed on the upper right side of the tank cover 3, and a piston 11 is movably installed inside the air cylinder 10. A first one-way valve 12 is installed on the left side of the air cylinder 10, a second one-way valve 14 is installed at the bottom of the air cylinder 10, an air bag 15 is installed below the air cylinder 10, and a second valve 13 is set on the right side of the second one-way valve 14, and the second valve 13 is connected to the air bag 15.

[0022] Working principle: When using the sampling device of this bioreactor, firstly, the piston 11 is pushed upward, and external gas enters the interior of the gas cylinder 10 through the first one-way valve 12. Then, the piston 11 is pushed downward, and gas enters the interior of the gas bladder 15 through the second one-way valve 14. The gas bladder 15 deforms, its volume increases, and it compresses the space inside the tank 1, increasing the internal pressure. Next, the controller controls the electric push rod 8 to move the lifting tube 2 downward, so that the lifting tube 2 is inserted below the liquid surface. Then, the controller controls the Peltier 21, temperature sensor 24, and fan 19 to be powered on. The Peltier 21, in conjunction with the second heat-conducting plate 22, cools the water inside the cooling water tank 5. The temperature sensor 24 monitors the temperature inside the cooling water tank 5 in real time. The temperature of the part, in conjunction with Peltier 21, controls the temperature inside the cooling water tank 5 to a certain level. At the same time, the fan 19 dissipates heat from the first heat-conducting plate 20. Peltier 21 is existing technology and will not be described in detail here. Next, the first valve 7 is opened, and the liquid inside the tank 1 is discharged through the lifting pipe 2 and the liquid collection pipe 6 under pressure. When the liquid passes through the heat-conducting pipe 23, it is cooled down. Then, after sampling is completed, the first valve 7 is closed, and the electric push rod 8 is controlled by the controller to move the lifting pipe 2 upward to the liquid surface. The liquid inside the liquid collection pipe 6 falls back into the tank 1. Finally, the second valve 13 is opened to connect the air bag 15 with the outside, thereby restoring the air pressure inside the tank 1. This is the working principle of the sampling device of the bioreactor.

[0023] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A sampling device for a bioreactor, characterized in that, include: A tank body (1) is provided, with a tank cover (3) installed on top of the tank body (1). A heat sink (4) is installed on the upper left side of the tank cover (3). A fan (19) is installed on the upper part of the heat sink (4). A Peltier (21) is installed on the lower part of the heat sink (4). A first heat-conducting fin (20) is installed on the hot end of the Peltier (21), and a second heat-conducting fin (22) is installed on the cold end of the Peltier (21). A cooling water tank is installed on the right side of the heat sink (4). 5) A liquid extraction pipe (6) is installed inside the cooling water tank (5). A heat conduction pipe (23) is installed inside the liquid extraction pipe (6). A temperature sensor (24) is installed on the inner wall of the cooling water tank (5). An electric push rod (8) is installed on the right side of the cooling water tank (5). A first valve (7) is installed above the liquid extraction pipe (6). A lifting pipe (2) is installed below the liquid extraction pipe (6). A sealing ring (18) is installed at the lower end of the outer surface of the liquid extraction pipe (6).

2. The sampling device for a bioreactor according to claim 1, characterized in that, The heat pipe (23) runs through the interior of the cooling water tank (5).

3. The sampling device for a bioreactor according to claim 1, characterized in that, The second heat-conducting plate (22) passes through the inner wall of the heat dissipation cylinder (4) and the cooling water tank (5) in sequence.

4. The sampling device for a bioreactor according to claim 1, characterized in that, The output end of the electric push rod (8) is fixedly connected to the lifting tube (2), and the sealing ring (18) is in contact with the inner wall of the lifting tube (2). The lifting tube (2) moves up and down along the liquid collection tube (6) through the electric push rod (8).

5. A sampling device for a bioreactor according to claim 1, characterized in that, The can lid (3) is also provided with: A stirring motor (9) is installed in the upper middle part of the tank cover (3). A drive shaft (16) is installed at the output end of the stirring motor (9). A stirring rod (17) is installed on the outer surface of the drive shaft (16). The stirring rod (17) rotates inside the tank (1) through the stirring motor (9) and the drive shaft (16).

6. A sampling device for a bioreactor according to claim 1, characterized in that, The can lid (3) is also provided with: An air cylinder (10) is installed on the upper right side of the canister cover (3). A piston (11) is movably installed inside the air cylinder (10). A first one-way valve (12) is installed on the left side of the air cylinder (10). A second one-way valve (14) is installed at the bottom of the air cylinder (10). An air bag (15) is installed below the air cylinder (10). A second valve (13) is provided on the right side of the second one-way valve (14). The second valve (13) is connected to the air bag (15).

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