A multi-depth-of-field imaging bacterial detection microscope

Abstract

The utility model discloses a kind of multi-DOF imaging bacteria detection microscopes, including adjusting mechanism and spectrometer detection mechanism, the outside of adjusting mechanism is equipped with shielding box, the front end of adjusting mechanism is equipped with spectrometer detection mechanism, the spectrometer detection mechanism includes transmission frame, the front end of transmission frame is fixedly installed with two light trapping chips, the front end of two the light trapping chips is slidably connected with first lens barrel and second lens barrel, one mainboard is fixedly installed in the side of first lens barrel and second lens barrel, multi-DOF camera is built-in in the mainboard, first lens barrel is located in the top of second lens barrel, the front end of second lens barrel is equipped with second light guide seat.The utility model is layered and twice reflected to bacteria imaging light, can be convenient for twice microscopic imaging to bacteria, and it is convenient to adjust microscopic imaging effect, imaging is more accurate, different depth of field image can be collected simultaneously and fused integration.

Description

Technical Field

[0001] This utility model relates to the field of microscope technology, specifically to a multi-depth-of-field imaging bacterial detection microscope. Background Technology

[0002] The principle of a microscope is primarily based on optics, using a lens system to magnify and image tiny objects. A microscope typically consists of an eyepiece, objective lens, stage, and mirror. Both the eyepiece and objective lens are convex lenses. The objective lens has a shorter focal length and is used to magnify the object and form an inverted real image. The eyepiece then magnifies this real image again to form an upright virtual image. Ultimately, the magnified image of the object can be observed by the human eye. Microscopes can magnify tiny objects invisible to the naked eye, such as bacteria and cells, helping scientists and researchers to study these microscopic structures in detail.

[0003] When using existing microscopes to detect bacteria, the images are often unclear due to the influence of light, and it is not convenient to adjust the microscopic imaging effect of bacteria. At the same time, it is not possible to integrate the acquired images. Therefore, it does not meet the current needs. To address this, we propose a multi-depth-of-field imaging bacterial detection microscope. Summary of the Invention

[0004] The purpose of this invention is to provide a multi-depth-of-field imaging bacterial detection microscope to solve the problems mentioned in the background art, such as the difficulty in clear bacterial imaging caused by light influence when existing microscopes are used to detect bacteria, and the inconvenience in adjusting the bacterial microscopic imaging effect.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a multi-depth-of-field imaging bacterial detection microscope, comprising an adjustment mechanism and a spectroscopic detection mechanism. A shielding box is installed on the outside of the adjustment mechanism, and a spectroscopic detection mechanism is installed at the front end of the adjustment mechanism. The spectroscopic detection mechanism includes a transmission frame, and two light-catching chips are fixedly installed at the front end of the transmission frame. A first lens tube and a second lens tube are slidably connected to the front ends of the two light-catching chips. A main board is fixedly installed on one side of the first lens tube and the second lens tube. The main board has a built-in multi-depth-of-field camera. The first lens tube is located above the second lens tube. A second light guide seat is installed at the front end of the second lens tube, and a reflective lens is installed on the lower end surface of the second light guide seat. A first light guide seat is installed at the front end of the first lens tube, and a spectroscopic lens is installed on the lower end surface of the first light guide seat. A lens is installed at the upper end of the first light guide seat.

[0006] Preferably, the adjustment mechanism includes a stepper motor, the output end of which is connected to a transmission screw via a coupling, a push plate is mounted on the outer side of the transmission screw, and a guide frame is slidably connected to the outer side of the push plate.

[0007] Preferably, the shielding box includes a protective base, a light source frame is fixedly installed at the front end of the protective base, a mounting base is fixedly installed at the upper end of the protective base, and a loading surface is provided on the front of the upper surface of the mounting base.

[0008] Preferably, the stepper motor is fixedly connected to the protective seat, the transmission screw is threadedly connected to the push plate, one end of the push plate passes through the guide frame and is fixedly connected to the rear end of the transmission frame, and the guide frame is fixedly connected to the mounting base.

[0009] Preferably, the first and second lens barrels are coaxial with the two light-collecting chips, the main board is electrically connected to the stepper motor and the two light-collecting chips, the length of the first lens barrel is greater than the length of the second lens barrel, and the front ends of the first and second lens barrels are fixedly connected to the protective base through the first light guide base and the second light guide base, respectively.

[0010] Preferably, the bottom end of the lens is connected to the first light guide seat by a thread, the middle part of the object placement surface is provided with a through hole, the upper end of the lens is inserted into the inside of the through hole, the beam splitter is inserted between the first light guide seat and the second light guide seat, the beam splitter is fixedly connected to the first light guide seat and the reflector is fixedly connected to the second light guide seat, and the lens is perpendicular to the axis of the first lens barrel and the second lens barrel.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] 1. This utility model uses a lens to filter and guide the light illuminating the substrate to the inner side of the first light guide seat. A beam splitter can reflect and transmit the light transmitted by the lens, thereby achieving layered processing of the light. The lens is perpendicular to the first and second lens barrels, so that the light reflected by the beam splitter through the first lens barrel is transmitted to one of the light-catching chips. The reflector can reflect the layered light and transmit it to another light-catching chip through the second lens barrel.

[0013] 2. This utility model uses two light-catching chips to receive two layers of light and convert them into image information, which is then transmitted to the motherboard. The motherboard adjusts the position of the light-catching chips based on the image clarity. A stepper motor drives a push plate to slide linearly back and forth under the guidance of a guide frame via a transmission screw. The push plate then drives the two light-catching chips to slide and extend relative to the first and second lens barrels through the transmission frame, thereby adjusting the distance between the beam splitter and reflector and the two light-catching chips. This satisfies the detection operation of microscopic imaging of bacteria at different levels. The motherboard has multiple depth-sensing cameras built in, enabling the fusion and output of images with different depths. Attached Figure Description

[0014] Figure 1This is a schematic diagram of the overall structure of this utility model;

[0015] Figure 2 This is an exploded view of the shielding box of this utility model;

[0016] Figure 3 This is a schematic diagram of the adjustment mechanism of this utility model;

[0017] Figure 4 This is an exploded structural diagram of the spectrophotometer detection mechanism of this utility model.

[0018] In the diagram: 1. Shielding box; 101. Protective base; 102. Mounting base; 103. Light source frame; 104. Loading surface; 2. Adjustment mechanism; 201. Stepper motor; 202. Transmission screw; 203. Guide frame; 204. Push plate; 3. Beam splitting and detection mechanism; 301. First lens barrel; 302. Second lens barrel; 303. Lens; 304. First light guide base; 305. Second light guide base; 306. Light capturing chip; 307. Transmission frame; 308. Main board; 309. Beam splitter; 310. Reflector. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0020] Please see Figure 1 and Figure 2 This utility model provides an embodiment of a multi-depth-of-field imaging bacterial detection microscope, including an adjustment mechanism 2 and a spectroscopic detection mechanism 3. A shielding box 1 is installed on the outside of the adjustment mechanism 2. The shielding box 1 includes a protective base 101. A light source frame 103 is fixedly installed at the front end of the protective base 101. A mounting base 102 is fixedly installed at the upper end of the protective base 101. A material placement surface 104 is provided at the front of the upper surface of the mounting base 102. The protective base 101 and the mounting base 102 facilitate the shielding installation of the adjustment mechanism 2 and the spectroscopic detection mechanism 3, thereby improving the microscopic imaging effect.

[0021] Please see Figure 3 and Figure 4The front end of the adjustment mechanism 2 is equipped with a beam splitting detection mechanism 3. The beam splitting detection mechanism 3 includes a transmission frame 307. Two light-catching chips 306 are fixedly installed at the front end of the transmission frame 307. The front ends of the two light-catching chips 306 are slidably connected to a first lens tube 301 and a second lens tube 302. The length of the first lens tube 301 is greater than the length of the second lens tube 302. The first lens tube 301 and the second lens tube 302 are coaxial with the two light-catching chips 306. A main board 308 is fixedly installed on one side of the first lens tube 301 and the second lens tube 302. A multi-depth camera is built into the main board 308, which enables the fusion and output of images with different depths.

[0022] The first lens tube 301 is located above the second lens tube 302. A second light guide seat 305 is installed at the front end of the second lens tube 302, and a first light guide seat 304 is installed at the front end of the first lens tube 301. The front ends of the first lens tube 301 and the second lens tube 302 are fixedly connected to the protective seat 101 through the first light guide seat 304 and the second light guide seat 305 respectively. The first lens tube 301 and the second lens tube 302 can transmit layered light synchronously.

[0023] A reflector 310 is mounted on the lower end face of the second light guide seat 305, and a beam splitter 309 is mounted on the lower end face of the first light guide seat 304. The beam splitter 309 is inserted between the first light guide seat 304 and the second light guide seat 305. The beam splitter 309 and the first light guide seat 304 and the reflector 310 and the second light guide seat 305 are both fixedly connected. A lens 303 is mounted on the upper end of the first light guide seat 304. The bottom end of the lens 303 is connected to the first light guide seat 304 by a thread. A through hole is provided in the middle of the object placement surface 104. The upper end of the lens 303 is inserted into the inside of the through hole. The lens 303 is perpendicular to the axis of the first lens barrel 301 and the second lens barrel 302. The beam splitter 309 can reflect and transmit the light transmitted by the lens 303, thereby realizing the layering of light. The reflector 310 can reflect the layered light.

[0024] Please see Figures 2 to 4The adjustment mechanism 2 includes a stepper motor 201. The main board 308 is electrically connected to the stepper motor 201 and two light-harvesting chips 306. The stepper motor 201 is fixedly connected to the protective base 101. The output end of the stepper motor 201 is connected to a transmission screw 202 via a coupling. A push plate 204 is mounted on the outside of the transmission screw 202. The transmission screw 202 and the push plate 204 are connected by threads. A guide frame 203 is slidably connected to the outside of the push plate 204. One end of the push plate 204 passes through the guide frame 203 and is fixedly connected to the rear end of the transmission frame 307. The guide frame 203 is fixedly connected to the mounting base 102. The stepper motor 201 drives the two light-harvesting chips 306 to slide synchronously through the transmission screw 202, the push plate 204, and the transmission frame 307, thereby adjusting the distance between the beam splitter 309 and the reflector 310 and the two light-harvesting chips 306, and meeting the detection operation of microscopic imaging of bacteria at different degrees.

[0025] In use, the adjustment mechanism 2 and the spectrophotometer 3 are installed inside the protective base 101 and the mounting base 102. When performing microscopic detection of bacteria, the slide containing the bacteria to be detected is placed on the slide placement surface 104. The power is turned on, and the slide is irradiated by the light source on the light source holder 103. A through hole is provided in the middle of the slide placement surface 104. The upper end of the lens 303 is inserted into the inside of the through hole, so that the light irradiating the slide can be filtered by the lens 303 and guided to the inside of the first light guide base 304.

[0026] A beam splitter 309 is fixedly installed on the lower end face of the first light guide 304, so that the light transmitted by the lens 303 can be reflected and transmitted through the beam splitter 309, thereby realizing the layering of light. The lens 303 is perpendicular to the first lens barrel 301 and the second lens barrel 302, so that the light reflected by the beam splitter 309 through the first lens barrel 301 is transmitted to one of the light-catching chips 306. A reflector 310 is fixedly installed on the lower end face of the second light guide 305, so that the layered light can be reflected through the reflector 310 and transmitted to another light-catching chip 306 through the second lens barrel 302.

[0027] The motherboard 308 is electrically connected to the stepper motor 201 and two light-collecting chips 306, so that the two light-collecting chips 306 can receive the two layers of light and convert them into image information and transmit it to the motherboard 308. The motherboard 308 adjusts the position of the light-collecting chips 306 according to the clarity of the image.

[0028] Specifically, the stepper motor 201, supported by the protective seat 101, drives the push plate 204 to slide linearly back and forth under the guidance of the guide frame 203 via the transmission screw 202. Then, the push plate 204 drives the two light-catching chips 306 to slide and extend relative to the first lens barrel 301 and the second lens barrel 302 through the transmission frame 307, thereby adjusting the distance between the beam splitter 309 and the reflector 310 and the two light-catching chips 306, which meets the detection operation of microscopic imaging of bacteria at different degrees. The motherboard 308 has a built-in multi-depth camera, which can fuse and output images of different depths collected during the adjustment of the light-catching chips 306.

[0029] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A multi-depth-of-field imaging bacterial detection microscope, comprising an adjustment mechanism (2) and a spectroscopic detection mechanism (3), characterized in that: A shielding box (1) is installed on the outside of the adjustment mechanism (2), and a spectroscopic detection mechanism (3) is installed at the front end of the adjustment mechanism (2). The spectroscopic detection mechanism (3) includes a transmission frame (307). Two light-collecting chips (306) are fixedly installed at the front end of the transmission frame (307). A first lens barrel (301) and a second lens barrel (302) are slidably connected to the front ends of the two light-collecting chips (306). A main board (308) is fixedly installed on one side of the first lens barrel (301) and the second lens barrel (302). The plate (308) has a built-in multi-depth camera. The first lens barrel (301) is located above the second lens barrel (302). The front end of the second lens barrel (302) is equipped with a second light guide seat (305). The lower end face of the second light guide seat (305) is equipped with a reflective lens (310). The front end of the first lens barrel (301) is equipped with a first light guide seat (304). The lower end face of the first light guide seat (304) is equipped with a beam splitter (309). The upper end of the first light guide seat (304) is equipped with a lens (303).

2. The multi-depth-of-field imaging bacterial detection microscope according to claim 1, characterized in that: The adjustment mechanism (2) includes a stepper motor (201), the output end of which is connected to a transmission screw (202) via a coupling, a push plate (204) is mounted on the outside of the transmission screw (202), and a guide frame (203) is slidably connected to the outside of the push plate (204).

3. The multi-depth-of-field imaging bacterial detection microscope according to claim 2, characterized in that: The shielding box (1) includes a protective base (101), a light source frame (103) is fixedly installed at the front end of the protective base (101), and a mounting base (102) is fixedly installed at the upper end of the protective base (101). The front part of the upper surface of the mounting base (102) is provided with a loading surface (104).

4. The multi-depth-of-field imaging bacterial detection microscope according to claim 3, characterized in that: The stepper motor (201) is fixedly connected to the protective seat (101), the transmission screw (202) is connected to the push plate (204) by a thread, one end of the push plate (204) passes through the guide frame (203) and is fixedly connected to the rear end of the transmission frame (307), and the guide frame (203) is fixedly connected to the mounting seat (102).

5. The multi-depth-of-field imaging bacterial detection microscope according to claim 4, characterized in that: The first lens barrel (301) and the second lens barrel (302) are coaxial with two light-collecting chips (306). The main board (308) is electrically connected to the stepper motor (201) and the two light-collecting chips (306). The length of the first lens barrel (301) is greater than that of the second lens barrel (302). The front ends of the first lens barrel (301) and the second lens barrel (302) are fixedly connected to the protective base (101) through the first light guide base (304) and the second light guide base (305), respectively.

6. The multi-depth-of-field imaging bacterial detection microscope according to claim 5, characterized in that: The bottom end of the lens (303) is connected to the first light guide seat (304) by a thread. The middle part of the object placement surface (104) is provided with a through hole. The upper end of the lens (303) is inserted into the inside of the through hole. The beam splitter (309) is inserted between the first light guide seat (304) and the second light guide seat (305). The beam splitter (309) is fixedly connected to the first light guide seat (304) and the reflector (310) is fixedly connected to the second light guide seat (305). The lens (303) is perpendicular to the axis of the first lens barrel (301) and the second lens barrel (302).

Images