A fluorescent quantum dot detection optical microscope automatic scanning system

Abstract

The utility model discloses a fluorescent quantum dot detection optical microscope automatic scanning system, including gantry and setting two axle translation stage module on the gantry, cantilevered frame, two axle translation stage module includes X axle translation mechanism and Y axle translation mechanism, object table module is located two axle translation stage module upper portion, including object table body and antiskid clamping mechanism, cantilevered frame and gantry rigid connection, integrated imaging positioning module, scanning focusing module, the light path coaxial or light path space matching of imaging positioning module and scanning focusing module, and the detection area is coincident with the sample slide position, this system adopts high accuracy linear motor and grating ruler reading head closed loop feedback, and positioning accuracy reaches plus or minus 100nm, and imaging positioning module can find sample slide position quickly, reduces manual search time, realizes the quick positioning of fluorescent quantum dot sample, simplifies the operation process, is applicable to scientific research and industrial detection scene.

Description

Technical Field

[0001] This utility model belongs to the field of optical microscope detection technology, and relates to automated scanning, positioning and imaging of fluorescent quantum dot samples, specifically to an automatic scanning system for fluorescent quantum dot detection optical microscope. Background Technology

[0002] Fluorescent quantum dots, due to their unique photoelectric properties, have important applications in display technology, biolabeling, and solar cells. However, traditional optical microscopy suffers from the following limitations in the detection and analysis of fluorescent quantum dots:

[0003] 1. Low positioning efficiency: Traditional optical microscopes rely on manual operation of the stage to move and to find the target area by visual inspection or low magnification. Positioning a single sample usually takes 5-10 minutes, which is time-consuming and easy to miss tiny quantum dot aggregation areas, affecting the representativeness of the statistical results.

[0004] 2. Insufficient focusing accuracy: The nanoscale size of quantum dots requires sub-pixel focusing accuracy, but the manual focusing of traditional optical microscopes is affected by the operator's experience, which can easily lead to blurred images or signal loss.

[0005] 3. Low level of automation: The scanning process requires manual intervention, making it impossible to continuously scan and detect batches of samples, which is difficult to meet the high-throughput requirements of industrial testing.

[0006] Currently, although some improved devices have translation functions, they lack integrated design for macroscopic positioning and autofocus, making it difficult to meet the requirements of "high resolution and high repeatability" for fluorescence quantum dot detection. Utility Model Content

[0007] To address the aforementioned problems, the main objective of this invention is to design an automated scanning system for a fluorescent quantum dot detection optical microscope, thereby solving the issues of low positioning efficiency, insufficient focusing accuracy, and low automation in the quantum dot characterization process using traditional optical microscopes.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] An automated scanning system for detecting fluorescent quantum dots using an optical microscope includes:

[0010] stand;

[0011] A two-axis translation stage module, fixed on a frame, includes an X-axis translation mechanism and a Y-axis translation mechanism;

[0012] The stage module, located on top of the two-axis translation stage module, includes a stage body for placing a glass slide carrying a fluorescent quantum dot sample, and an anti-slip clamping mechanism for holding the sample glass slide.

[0013] The cantilever frame is rigidly connected to the test bench and integrates an imaging positioning module and a scanning focusing module.

[0014] The imaging positioning module and the scanning focusing module have coaxial or spatially matched optical paths, and the detection area coincides with the position of the sample slide on the stage body.

[0015] As a further description of this utility model, the guide surfaces of the X-axis translation mechanism and the Y-axis translation mechanism are perpendicular to each other.

[0016] As a further description of this utility model, the X-axis translation mechanism includes an X-axis linear motor that drives the stage module to move horizontally, an X-axis cross guide rail, and an X-axis slide; the Y-axis translation mechanism includes a Y-axis linear motor that drives the stage module to move vertically, a Y-axis cross guide rail, and a Y-axis slide.

[0017] As a further description of this utility model, the mover of the X-axis linear motor is rigidly connected to the X-axis slide, the stator is fixed to the first mounting base, the X-axis cross guide rail is fixed above the first mounting base, and the X-axis slide and the X-axis cross guide rail are in sliding cooperation.

[0018] The mover of the Y-axis linear motor is rigidly connected to the Y-axis slide, the stator is fixed to the second mounting base, the Y-axis cross guide rail is fixed above the second mounting base, and the Y-axis slide and the Y-axis cross guide rail are in sliding engagement.

[0019] The upper surface of the Y-axis slide is fixedly connected to the lower part of the first mounting base, and the second mounting base is fixed to the frame.

[0020] As a further description of this utility model, a grating ruler reading head is installed above the first mounting base along the X-axis direction, and a grating ruler reading head is set above the second mounting base along the Y-axis direction; the resolution of the grating ruler reading head is set to 50nm.

[0021] As a further description of this utility model, the stage body is fixed above the X-axis slide, and an anti-slip clamping mechanism is provided on one side of the stage.

[0022] The anti-slip clamping mechanism includes a rotating clamping plate, which is hinged to the stage body via a lever shaft. One end of the rotating clamping plate is rotated and pressed against the upper surface of the sample slide, and the other end is connected to the X-axis slide via a tension spring. A lever idler wheel is provided at its end.

[0023] As a further description of this utility model, the imaging positioning module is installed above or to the side of the cantilever frame, and includes an optical camera whose field of view covers the position of the sample slide on the stage body.

[0024] As a further description of this utility model, the scanning and focusing module includes an optical microscope scanning unit, an autofocus unit, and a fluorescence excitation component.

[0025] Compared with the prior art, the technical effects of this utility model are as follows:

[0026] This invention provides an automated scanning system for fluorescent quantum dot detection optical microscopes, including a stage and a two-axis translation stage module and a cantilever frame mounted on the stage. The two-axis translation stage module includes an X-axis translation mechanism and a Y-axis translation mechanism. The stage module is mounted on the upper part of the two-axis translation stage module via a mounting plate and includes a stage body and an anti-slip clamping mechanism. The cantilever frame is rigidly connected to the stage. An imaging positioning module and a scanning focusing module are integrated. The optical paths of the imaging positioning module and the scanning focusing module are coaxial or spatially matched, and the detection area coincides with the position of the sample slide. The two-axis translation stage module of this system adopts a high-precision linear motor + 50nm grating ruler reading head closed-loop feedback, achieving a positioning accuracy of ±100nm. The imaging positioning module can quickly locate the sample slide position, reducing manual search time and realizing rapid positioning of fluorescent quantum dot samples, simplifying the operation process, and is suitable for scientific research and industrial testing scenarios. Attached Figure Description

[0027] Figure 1 This is an overall structural view of the present invention;

[0028] Figure 2 This is a top view of the two-axis translation stage module of this utility model;

[0029] Figure 3 This is a structural view of the stage and scanning focusing module of this utility model.

[0030] In the diagram, 1. Platform, 2. Two-axis translation stage module, 21. X-axis linear motor, 22. X-axis cross guide rail, 23. X-axis slide, 24. Y-axis linear motor, 25. Y-axis cross guide rail, 26. Y-axis slide, 27. First mounting base, 28. Second mounting base, 3. Cantilever frame, 4. Stage module, 41. Stage body, 42. Anti-slip clamping mechanism, 43. Rotary clamping plate, 44. Lever shaft, 45. Tension spring, 46. Lever idler wheel, 5. Imaging positioning module, 6. Scanning focusing module, 7. Grating ruler reading head. Detailed Implementation

[0031] The present invention will now be described in detail with reference to the accompanying drawings:

[0032] In one embodiment of this utility model, an automated scanning system for fluorescent quantum dot detection optical microscope is disclosed, such as... Figure 1-3As shown, the system includes a stage 1, a two-axis translation stage module 2 and a cantilever frame 3 mounted on the stage 1; the two-axis translation stage module 2 is fixed to the stage 1 and includes an X-axis translation mechanism and a Y-axis translation mechanism; the stage module 4 is located on the upper part of the two-axis translation stage module 2 and includes a stage body 41 for placing a glass slide carrying a fluorescent quantum dot sample and an anti-slip clamping mechanism 42 for holding the sample glass slide; the cantilever frame 3 is rigidly connected to the stage 1 and integrates an imaging positioning module 5 and a scanning focusing module 6; wherein, the optical paths of the imaging positioning module 5 and the scanning focusing module 6 are coaxial or spatially matched, and the detection area coincides with the position of the sample glass slide on the stage body 41.

[0033] Specifically, in this embodiment, the above-mentioned components will be described in detail as follows:

[0034] Two-axis translation stage module 2: The guide surfaces of the X-axis translation mechanism and the Y-axis translation mechanism are perpendicular to each other. Through the linkage of the electronic control module, the stage module 4 can move to any position in the plane.

[0035] In this embodiment, the X-axis translation mechanism includes an X-axis linear motor 21, an X-axis cross guide rail 22, and an X-axis slide 23 that drive the stage module 4 to move horizontally; the Y-axis translation mechanism includes a Y-axis linear motor 24, a Y-axis cross guide rail 25, and a Y-axis slide 26 that drive the stage module 4 to move vertically. Specifically, the mover of the X-axis linear motor 21 is rigidly connected to the X-axis slide 23, and the stator is fixed to the first mounting base 27. The X-axis cross guide rail 22 is fixed above the first mounting base 27, and the X-axis slide 23 slides in cooperation with the X-axis cross guide rail 22. Through the movement of the X-axis linear motor 21, the X-axis slide 23 moves relative to the first mounting base 27, thereby realizing the horizontal movement of the stage module 4; the mover of the Y-axis linear motor 24 is rigidly connected to the Y-axis slide 26, and the stator is fixed to the second mounting base 27. The mounting base 28 is fixed above the Y-axis cross guide rail 25. The Y-axis slide 26 slides in conjunction with the Y-axis cross guide rail 25. The movement of the Y-axis linear motor 24 drives the Y-axis slide 26 to move relative to the second mounting base 28, thereby realizing the longitudinal movement of the stage module 4. At the same time, in order to ensure the linkage between the X-axis translation mechanism and the Y-axis translation mechanism, the upper surface of the Y-axis slide 26 is fixedly connected to the lower part of the first mounting base 27, and the second mounting base 28 is fixed to the frame 1.

[0036] It should also be noted that the X-axis linear motor 21 and the Y-axis linear motor 24 are high-precision linear motors; in addition, a grating ruler reading head 7 is installed above the first mounting base 27 along the X-axis direction, and a grating ruler reading head 7 is set above the second mounting base 28 along the Y-axis direction; the resolution of the grating ruler reading head 7 is set to 50nm; the combination of high-precision linear motors and grating ruler reading heads improves positioning accuracy.

[0037] Cantilever Frame 3 (Marble Cantilever Frame): Serves as the system support frame, fixing and integrating the electrical control module and optical path system. The electrical control module uses an STM32 microcontroller or PLC controller and communicates with the host computer via a USB interface.

[0038] Stage module 4: includes stage body 41 and anti-slip clamping mechanism 42; stage body 41 is used to place glass slides carrying fluorescent quantum dot samples, and anti-slip clamping mechanism 42 is used to ensure the stability of sample glass slides.

[0039] Specifically, the surface of the stage body 41 is made of anodized aluminum alloy and is fixed to the upper part of the X-axis slide 23 of the two-axis translation stage module 2. An anti-slip clamping mechanism 42 is provided on one side edge to prevent the sample slide from slipping. The anti-slip clamping mechanism 42 includes a rotating clamping plate 43, which is hinged to the stage body 41 through a lever shaft 44. One end of the rotating clamping plate 43 is rotated and pressed against the upper surface of the sample slide, and the other end is connected to the X-axis slide 23 through a tension spring 45. A lever idler wheel 46 is provided at its end.

[0040] Imaging and positioning module 5: Installed above or to the side of the cantilever frame 3, including an optical camera whose field of view covers the sample slide position on the stage body 41. The optical camera is a low-magnification optical camera (or CCD) used to capture macroscopic images of the slide. The camera's field of view covers the positioning area of ​​the stage body 41, clearly showing the distribution position of the sample on the slide.

[0041] Specifically, in this embodiment, the optical camera is a 5-megapixel industrial camera, equipped with a 12mm focal length lens, and the field of view covers a 75mm×25mm glass slide.

[0042] Scanning and focusing module 6: includes an optical microscope scanning unit, an autofocus unit, and a fluorescence excitation component.

[0043] Microscope scanning unit: Employs a secondary electron detector to enhance the simultaneous acquisition capability of quantum dot surface morphology and fluorescence signals; integrates an electron gun and lens system for high-resolution scanning of fluorescent quantum dots; the scanning path planning adopts a grid traversal algorithm, which can customize the scanning area and step size (e.g., 3mm×3mm).

[0044] Autofocus unit: including laser rangefinder or image contrast detection module, which adjusts the focal length in real time through algorithm to ensure clear image. The autofocus algorithm is based on image gradient analysis, such as the Laplacian operator, and achieves closed-loop adjustment by comparing the image sharpness at different focal lengths.

[0045] Fluorescence excitation component: This component incorporates a specific wavelength excitation light source, such as an ultraviolet or visible LED, to excite the quantum dot fluorescence signal. In this embodiment, the excitation light source is an LED matching the emission wavelength of the quantum dot, such as 365nm ultraviolet light, and is paired with a bandpass filter to improve the signal-to-noise ratio.

[0046] It should be noted that the imaging positioning module 5 and scanning focusing module 6 described above are conventional settings in the prior art, including but not limited to the settings and components described in this embodiment. Specifically, in this embodiment, the imaging positioning module 5 consists of a camera (Daheng MER-630-60U3C-L), a lens (Zhongke Lianchuang HM1214MP5), and a ring light from top to bottom; the lens is manually focused and fixed; when the imaging positioning module 5 is working, the ring light is powered on and illuminated; the camera acquires an image of the positioning position; and by mapping the coordinates of the top left pixel 0 point of the image with the image acquired by the scanning camera, the macroscopic positioning and the actual position of the stage module 4 can be mapped point-to-point. The scanning and focusing module 6 mainly consists of an objective lens (Olympus 40x0.75), a two-dimensional angle adjuster, a 20nm grating ruler reading head (Dalian Rongshu RU2LHEN03M), and a slide stage (LX70-L). The objective lens is fixed on the two-dimensional angle adjuster, and the angle of the objective lens is adjusted by adjusting the knob to ensure that it is perpendicular to the slide. The two-dimensional angle adjuster is fixed on the slide stage, and the slide stage can move up and down. The 20nm grating ruler reading head accurately feeds back the position of the slide stage, realizing a closed loop to ensure the Z-axis movement accuracy.

[0047] Based on the aforementioned disclosed automatic scanning system, its specific workflow includes the following:

[0048] 1. Macroscopic positioning: Place the sample slide on the stage body, and the two-axis translation stage module drives the stage body to move below the imaging positioning module. The optical camera takes a macroscopic picture to show the position of the sample slide.

[0049] 2. Automatic scanning: Based on the "scan" command, the two-axis translation stage module drives the stage body to move to the scanning area of ​​the optical microscope scanning unit;

[0050] 3. Autofocus: The autofocus unit adjusts the objective lens height in real time through laser ranging or image analysis until the clarity of the fluorescence image is maximized;

[0051] 4. Continuous scanning: Automatically completes full-area scanning according to a preset path (such as grid scanning) and records data such as fluorescence intensity.

[0052] The above content discloses the technical solution of this utility model, which has the following advantages compared with the prior art:

[0053] 1. Improved positioning efficiency: The two-axis translation stage module adopts a high-precision linear motor + 50nm grating ruler reading head closed-loop feedback, with a positioning accuracy of ±100nm. The imaging positioning module can quickly find the position of the sample slide, reduce manual search time, and improve positioning efficiency.

[0054] 2. High focusing accuracy: The autofocus unit achieves nanometer-level precision adjustment, suitable for high-resolution imaging of fluorescent quantum dots;

[0055] 3. Automated inspection: This structure, combined with software programs, supports unattended scanning, making it suitable for automated scanning inspection in scientific research or industrial quality control;

[0056] 4. Easy to operate: Reduces the professional requirements for operators.

[0057] The above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Any other modifications or equivalent substitutions made by those skilled in the art to the technical solution of this utility model, as long as they do not depart from the spirit and scope of the technical solution of this utility model, should be covered within the scope of the claims of this utility model.

Claims

1. An automated scanning system for fluorescent quantum dot detection optical microscopes, characterized in that, include: stand; A two-axis translation stage module, fixed on a frame, includes an X-axis translation mechanism and a Y-axis translation mechanism; The stage module, located on top of the two-axis translation stage module, includes a stage body for placing a glass slide carrying a fluorescent quantum dot sample, and an anti-slip clamping mechanism for holding the sample glass slide. The cantilever frame is rigidly connected to the test bench and integrates an imaging positioning module and a scanning focusing module. The imaging positioning module and the scanning focusing module have coaxial or spatially matched optical paths, and the detection area coincides with the position of the sample slide on the stage body.

2. The automatic scanning system for fluorescent quantum dot detection optical microscope according to claim 1, characterized in that: The guide surfaces of the X-axis translation mechanism and the Y-axis translation mechanism are perpendicular to each other.

3. An automated scanning system for fluorescent quantum dot detection optical microscopes according to claim 1 or 2, characterized in that: The X-axis translation mechanism includes an X-axis linear motor that drives the stage module to move horizontally, an X-axis cross guide rail, and an X-axis slide. The Y-axis translation mechanism includes a Y-axis linear motor that drives the stage module to move horizontally and longitudinally, a Y-axis cross guide rail, and a Y-axis slide.

4. The automatic scanning system for fluorescent quantum dot detection optical microscope according to claim 3, characterized in that: The mover of the X-axis linear motor is rigidly connected to the X-axis slide, the stator is fixed to the first mounting base, the X-axis cross guide rail is fixed above the first mounting base, and the X-axis slide and the X-axis cross guide rail are in sliding engagement. The mover of the Y-axis linear motor is rigidly connected to the Y-axis slide, the stator is fixed to the second mounting base, the Y-axis cross guide rail is fixed above the second mounting base, and the Y-axis slide and the Y-axis cross guide rail are in sliding engagement. The upper surface of the Y-axis slide is fixedly connected to the lower part of the first mounting base, and the second mounting base is fixed to the frame.

5. The automated scanning system for fluorescent quantum dot detection optical microscope according to claim 4, characterized in that: A grating ruler reading head is installed above the first mounting base along the X-axis direction, and a grating ruler reading head is installed above the second mounting base along the Y-axis direction. The resolution of the grating ruler reading head is set to 50nm.

6. The automated scanning system for fluorescent quantum dot detection optical microscope according to claim 3, characterized in that: The stage body is fixed above the X-axis slide, and an anti-slip clamping mechanism is provided on one side of the stage. The anti-slip clamping mechanism includes a rotating clamping plate, which is hinged to the stage body via a lever shaft. One end of the rotating clamping plate is rotated and pressed against the upper surface of the sample slide, and the other end is connected to the X-axis slide via a tension spring. A lever idler wheel is provided at its end.

7. The automated scanning system for fluorescent quantum dot detection optical microscope according to claim 1, characterized in that: The imaging positioning module is installed above or to the side of the cantilever frame and includes an optical camera whose field of view covers the position of the sample slide on the stage body.

8. The automatic scanning system for fluorescent quantum dot detection optical microscope according to claim 1, characterized in that: The scanning and focusing module includes an optical microscope scanning unit, an autofocus unit, and a fluorescence excitation component.

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