A shaker slide

Abstract

The utility model provides a kind of shaking mixed slide rack, belong to cell staining machine technical field.It includes slide rack body, and the recess for placing sample is equipped on one side of slide, heating platform is connected with body by pivot and is equipped with mounting groove, with the inclined angle's flow guide groove and stand, rotating assembly drives heating platform to rotate around pivot, drive assembly contains motor, synchronous belt and synchronous pulley to drive body horizontal movement, heating platform bottom has heating film and thermistor, also be equipped with diffuse reflection sensor.The slide rack can realize liquid shaking mixed to speed up antigen-antibody reaction, tilt heating platform cooperates with flow guide groove and waste liquid collection platform efficient cleaning, heating film precision temperature control, diffuse reflection sensor monitors slide state, stand prevents falling, improves dyeing efficiency, reliability and automation level.

Description

Technical Field

[0001] This invention belongs to the field of cell staining machine technology, specifically relating to a glass slide holder that shakes and mixes the contents. Background Technology

[0002] Immunohistochemical staining is widely used in clinical pathological diagnosis and research. It utilizes the principle of specific antigen-antibody binding to qualitatively, locally, and quantitatively study antigens within tissue cells. Intraoperative frozen section diagnosis requires pathologists to complete a rapid diagnosis within 30 minutes, but current immunohistochemical staining equipment suffers from long processing times. When the immunohistochemical reaction occurs at the solid-liquid interface, the probability of antigen-antibody collision is low, easily forming an "antibody depletion zone," resulting in reaction times that can last for tens of minutes or even hours.

[0003] In the prior art, such as the rapid immunohistochemical staining machine disclosed in Chinese invention patent with publication number CN 118243474A, the accelerator assembly uses a motor to drive the baffle to move in order to speed up the reaction. However, it has drawbacks such as a complex accelerator structure to speed up the reaction, the reaction port restricting the area where the operator can place tissue, and the precise installation height of the slide shaft may affect the slide preparation and the effect of the accelerator.

[0004] Therefore, there is an urgent need for a slide holder that is simple in structure, has high mixing efficiency, and is easy to clean, in order to meet the needs of rapid staining. Utility Model Content

[0005] The present invention aims to provide a shaker-mixing slide holder to solve the problems of long staining reaction time, easy liquid flow, incomplete cleaning and insufficient temperature control in the prior art, so as to achieve rapid mixing, efficient cleaning and precise temperature control.

[0006] This invention discloses a slide holder for shaking and mixing, comprising a slide holder body, and further comprising: a slide, one side of which has a groove for placing samples and serving as a staining reaction area; a heating stage, which is mounted on the body and one side of which is connected to the body via a rotating shaft, with the slide placed on the heating stage; a rotating assembly for driving the heating stage to rotate around the rotating shaft at different angles; and a driving assembly for driving the body to move horizontally. This slide holder allows the rotating assembly to tilt the heating stage for cleaning, the driving assembly to drive the body to move horizontally for shaking and mixing of liquids, the groove to prevent liquid from flowing away, and the heating stage to adapt to different reagent temperatures.

[0007] Preferably, the heating platform is provided with a mounting groove for placing the glass slide, and the two sides of the heating platform are also provided with guide grooves with a certain inclination angle.

[0008] By adopting the above technical solution and setting up the installation groove, the glass slide can be accurately positioned to ensure stable placement; the guide grooves with inclined angles on both sides can effectively guide the waste liquid to flow out quickly, prevent liquid accumulation and splashing, and improve cleaning efficiency and the cleanliness of the dyeing environment.

[0009] More preferably, the heating platform is also provided with a pair of columns, which are located on both sides of the mounting groove.

[0010] By adopting the above technical solution and setting up columns on both sides of the mounting groove, the slide can be effectively prevented from shifting or falling off during the staining process, ensuring the stability of the sample position and avoiding detection errors caused by slide shaking, thereby significantly improving the staining quality and the reliability of the detection results.

[0011] Preferably, it also includes a heating film disposed at the bottom of the heating platform, wherein a thermistor is disposed on the heating film.

[0012] By adopting the above technical solution, a heating film is set at the bottom of the heating stage and paired with a thermistor, which can precisely control the heating temperature, monitor temperature changes in real time, adapt to the reaction temperature requirements of different reagents, ensure that the antigen-antibody reaction is carried out at the optimal temperature, and effectively improve staining efficiency and reaction stability.

[0013] Preferably, the heating platform is also equipped with a diffuse reflection sensor.

[0014] By adopting the above technical solution and setting a diffuse reflection sensor on the heating stage, the installation status of the glass slide can be monitored in real time, accurately identifying whether the glass slide is correctly placed on the heating stage. This avoids equipment idling, reagent waste, and test failure caused by missing or improperly placed glass slides, significantly improving operational safety and equipment intelligence.

[0015] Preferably, the rotating assembly includes one of an electric cylinder, a telescopic cylinder, or a rotary motor.

[0016] By adopting the above technical solution, the rotating component is set as one of an electric cylinder, a telescopic cylinder, or a rotary motor. The driving method can be flexibly selected according to different application scenarios. The electric cylinder provides precise angle control, the cylinder achieves rapid response, and the rotary motor meets high torque requirements, adapting to diverse dyeing processes and significantly improving the equipment's versatility and automation level.

[0017] Preferably, the rotating component is an electric cylinder, with one end of the electric cylinder fixed to the base of the main body and the other end fixed to the base of the heating platform.

[0018] By adopting the above technical solution, the rotating component is set as an electric cylinder, and the two ends of the electric cylinder are fixed on the main body base and the heating table base respectively. The heating table can be driven to rotate smoothly around the rotating shaft by the precise extension and retraction of the electric cylinder, so as to achieve precise angle adjustment during cleaning. At the same time, the structure is compact and the transmission efficiency is high, which is suitable for the precise control requirements of automated dyeing process.

[0019] Preferably, the drive assembly includes a motor, a synchronous belt, and a synchronous pulley. The main body is disposed on the synchronous belt, and the motor is connected to the synchronous pulley through the synchronous belt. The motor drives the synchronous belt and the main body to move horizontally back and forth.

[0020] By adopting the above technical solution, when the motor rotates, it can precisely drive the body to move back and forth horizontally through the transmission of the synchronous belt and synchronous pulley, so as to realize the efficient shaking and mixing of liquid in the glass slide groove, accelerate the antigen-antibody reaction speed, and the transmission is smooth, with low noise and accurate positioning, which can meet the requirements for precise control of mixing frequency and amplitude during the staining process.

[0021] A further preferred embodiment includes a waste liquid collection platform located below the guide channel, with one end of the guide channel facing the waste liquid collection platform.

[0022] By adopting the above technical solution, the waste liquid can flow directly into the collection platform along the guide channel when the heating table is tilted, forming a closed waste liquid recycling path, avoiding waste liquid splashing and contaminating the equipment, and at the same time facilitating centralized treatment of waste liquid, thus improving the safety and environmental protection of the dyeing operation.

[0023] Compared with the prior art, the beneficial results of this utility model are as follows:

[0024] The heating stage is tilted by rotating components such as electric cylinders, which work in conjunction with the guide channel and waste liquid collection platform to achieve efficient cleaning. The motor drives the synchronous belt to move the slide holder horizontally, and the slide grooves enable liquid to be shaken and mixed, accelerating the antigen-antibody reaction. The heating film and thermistor precisely control the temperature, the diffuse reflection sensor monitors the slide status, and the column prevents the slide from falling off. The synergistic effect of these components improves staining efficiency, reliability and automation, solving the problems of slow reaction and incomplete cleaning in existing equipment. Attached Figure Description

[0025] The accompanying drawings are included to provide a further understanding of the embodiments and are incorporated in and constitute a part of this specification. The drawings illustrate embodiments and, together with the description, serve to explain the principles of the present invention. Other embodiments and many anticipated advantages of the embodiments will be readily recognized as they become better understood through reference to the following detailed description. Elements in the drawings are not necessarily to scale. The same reference numerals refer to corresponding similar parts.

[0026] Figure 1A schematic diagram of the overall structure of the shake-mixing slide holder according to the present invention is shown;

[0027] Figure 2 A top view of the body in the shake-mixing slide holder according to the present invention is shown;

[0028] Figure 3 A schematic diagram of the structure of a glass slide in a shaken and mixed glass slide holder according to an embodiment of the present invention is shown;

[0029] Figure 4 A cross-sectional schematic diagram of the entire slide holder for shaking and mixing according to an embodiment of the present invention is shown.

[0030] The meaning of each number in the diagram:

[0031] 1. Body; 1.1. Glass slide; 1.2. Heating stage; 1.3. Heating film; 1.4. Heating stage base; 1.5. Diffuse reflection sensor; 1.6. Spring; 1.7. Electric cylinder; 1.8. Mounting base; 1.9. Glass slide holder base; 1.10. Guide channel; 1.11. Column; 1.12. Groove;

[0032] 2. Waste liquid collection platform; 3. Motor; 4. Guide rail; 5. Synchronous belt; 6. Synchronous pulley. Detailed Implementation

[0033] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the relevant utility model and not intended to limit the scope of the utility model. Furthermore, it should be noted that, for ease of description, only the parts relevant to the utility model are shown in the accompanying drawings.

[0034] It should be noted that, where there is no conflict, the embodiments and features in the embodiments of this utility model can be combined with each other. The present utility model will now be described in detail with reference to the accompanying drawings and embodiments.

[0035] This novel shaking and mixing slide holder is mainly used in the immunohistochemical staining process. Its innovative structural design enables rapid liquid mixing, efficient cleaning, and precise temperature control. The specific implementation method is described in detail below with reference to the accompanying drawings:

[0036] like Figure 1 and Figure 2 As shown, this utility model proposes a shaker-mixing slide holder, including a slide holder body 1, a slide 1.1, a heating stage 1.2, a rotating assembly, a driving assembly, and a waste liquid collection platform 2. The slide 1.1 is placed in the mounting groove of the heating stage 1.2, which is connected to the body 1 via a rotating shaft. The rotating assembly drives the heating stage 1.2 to tilt, and the driving assembly drives the body 1 to move horizontally.

[0037] In this embodiment, as Figure 3 As shown, slide 1.1 is a rectangular slide with a groove 1.12 near its end. The depth of groove 1.12 is approximately 0.5–1 mm, used to place the tissue sample to be tested and as the staining reaction area. In actual use, all necessary reagents for the reaction are added into groove 1.12. The design of groove 1.12 prevents liquid from flowing away during shaking and mixing, while also limiting the sample diffusion range and improving staining accuracy.

[0038] Preferred, such as Figure 1 and Figure 4 As shown, the heating stage 1.2 is made of aluminum alloy with a smooth and flat surface. It features a mounting groove, a flow guide 1.10, columns 1.11, and a diffuse reflection sensor 1.5. The mounting groove matches the shape of the glass slide 1.1 and has a depth of 0.2–0.3 mm to ensure stable placement. The flow guide 1.10 is located on both sides of the heating stage 1.2, with an inclination angle of 5°–15° and a width of 2–3 mm, guiding the cleaning waste liquid into the waste liquid collection platform 2 below. A pair of cylindrical columns 1.11, 3–5 mm high, are located on both sides of the mounting groove to prevent the glass slide 1.1 from falling off due to shaking during the staining process. A conventional diffuse reflection sensor 1.5, installed in front of the heating stage 1.2 at a distance of 5–8 mm from the edge of the mounting groove, is used to detect whether the glass slide 1.1 is correctly placed.

[0039] Preferably, in one specific embodiment, a heating system comprising a heating film 1.3 and a thermistor is also provided. Specifically, a polyimide heating film 1.3, approximately 0.1–0.2 mm thick, is attached to the bottom surface of the heating platform 1.2, with a power of 50–100 W, capable of rapidly heating to 37–95°C. The thermistor is tightly bonded to the heating film 1.3, monitoring the temperature in real time and feeding back to the control system to achieve precise temperature control within ±0.5°C. The thermistor can be selected according to actual needs.

[0040] Furthermore, in this embodiment, the rotating component uses an electric cylinder 1.7, one end of which is fixed to the mounting base 1.8 of the slide holder base 1.9 of the main body 1 by bolts, and the other end is connected to the heating stage base 1.4 via a rotating shaft. The electric cylinder 1.7 has a stroke of 50-100mm, a maximum thrust of 50N, and can achieve precise angle adjustment from 0 to 45° through an electronic control system. Preferably, in a specific embodiment, an electric cylinder with a maximum thrust of approximately 20N and capable of precise angle adjustment from 0 to 20° is selected.

[0041] In other embodiments, the rotating component may also be a telescopic cylinder or a rotary motor.

[0042] In another embodiment, the rotating assembly uses a telescopic cylinder instead of the electric cylinder 1.7. The cylinder is hinged to the base of the body 1 via a U-shaped fork, and the other end is connected to the heating platform base 1.4 via a fisheye connector. The cylinder's air inlet and outlet are connected to a solenoid valve via air pipes. The solenoid valve is a two-position five-way valve, controlled by an electronic control system.

[0043] In the third embodiment, the rotating assembly uses a rotary motor to directly drive the heating platform 1.2 to rotate. The rotary motor is a commercially available stepper motor, whose output shaft is coaxially connected to the rotating shaft via a coupling. Both ends of the rotating shaft are fixed to the bracket of the main body 1 by bearings. The bottom of the heating platform 1.2 is fixed to the rotating shaft via a keyway structure to ensure synchronous rotation.

[0044] As a preferred embodiment, in the implementation driven by the electric cylinder 1.7, to further optimize the smoothness of the movement of the heating platform 1.2, a buffer spring 1.6 structure can be added at the connection between the electric cylinder 1.7 and the body 1, and the heating platform 1.2. For example, at the connection end between the electric cylinder 1.7 and the body 1: the spring seat is fixed on the base of the body 1, and the tail end of the electric cylinder 1.7 is connected to the spring 1.6 through a limiting bolt. At the connection end between the electric cylinder 1.7 and the heating platform base 1.4: the spring 1.6 seat is fixed on the base, and the piston rod head of the electric cylinder 1.7 is connected to the spring 1.6 through a ball joint and a limiting bolt.

[0045] This spring-buffered structure is also applicable to implementations using telescopic cylinders and rotary motor drives. In cylinder drives, spring 1.6 can be installed at the connection end between the piston rod and the heating platform 1.2 to reduce vibration caused by air pressure fluctuations; in rotary motor drives, spring 1.6 can be integrated into the coupling to compensate for torque shocks during motor start-up and shutdown.

[0046] By adding a spring 1.6 buffer structure, the slide 1.1 holder of this utility model significantly improves the stability of movement and the reliability of the equipment while ensuring efficient mixing and cleaning functions, making it particularly suitable for pathological diagnosis scenarios with high requirements for sample integrity.

[0047] Furthermore, such as Figure 1 As shown, in this embodiment, the drive assembly consists of a motor 3, a synchronous belt 5, and a synchronous pulley 6. The motor 3 is a commercially available stepper motor with a rated torque of 4 N·m; the synchronous pulley 6 has a diameter of 40–60 mm and 20–30 teeth; the synchronous belt 5 has a width of 10–15 mm and a pitch of 5 mm, and its toothed structure ensures transmission accuracy.

[0048] The motor 3 is connected to the synchronous pulley 6 via a coupling. The synchronous belt 5 is fixed to the slider at the bottom of the body 1. When the motor 3 rotates, it drives the synchronous belt 5 to move, thereby driving the body 1 to move horizontally back and forth along the guide rail 4 at a speed of 10 to 100 mm / s.

[0049] In other embodiments, in addition to using a synchronous belt 5 for transmission, the drive assembly can also use a lead screw and nut drive. The motor 3 is connected to the ball screw via a coupling, and the nut on the lead screw is fixed to the slider at the bottom of the body 1. When the motor 3 rotates, the nut moves linearly along the lead screw, driving the body 1 to move horizontally.

[0050] Alternatively, a linear motor 3 can be used to replace the rotary motor 3 and the transmission mechanism. The mover of the linear motor 3 is fixed to the bottom of the body 1, and the stator is mounted on the guide rail 4.

[0051] Next, I will briefly describe the workflow:

[0052] 1. Sample loading and initial detection:

[0053] The operator places the sample in the groove 1.12, and then places the glass slide 1.1 into the mounting slot of the heating stage 1.2. After the diffuse reflection sensor 1.5 detects the glass slide 1.1, the system activates the heating film 1.3 to raise the temperature to the preset value (e.g., 37°C).

[0054] 2. Staining and mixing process

[0055] Reagent addition: The external sample loading device injects the staining reagent into the groove 1.12;

[0056] Shaking and mixing: Motor 3 starts and drives the body 1 to move back and forth quickly through synchronous belt 5. The moving frequency is 2-5Hz and the moving distance is 20-50mm, which makes the liquid in the groove 1.12 shake and accelerates the antigen-antibody reaction.

[0057] It should be noted that, in practice, the moving frequency and moving distance can be adjusted according to the physicochemical properties of different reagents, staining conditions, etc., in order to obtain the best staining effect, and no specific restrictions are imposed here.

[0058] Temperature control: The thermistor monitors the temperature in real time and adjusts the power of the heating film 1.3 through PIT adjustment to maintain a constant temperature.

[0059] 3. Cleaning process

[0060] Waste liquid discharge: After dyeing is completed, the electric cylinder 1.7 extends, pushing the heating table 1.2 to tilt backward by 15 to 30 degrees around the axis of rotation. The waste liquid in the groove 1.12 flows into the waste liquid collection platform 2 along the guide channel 1.10.

[0061] Cleaning solution rinsing: The external cleaning device sprays cleaning solution onto the surface of the glass slide 1.1. At the same time, the electric cylinder 1.7 can extend and retract multiple times, allowing the heating stage 1.2 to switch between different angles, thereby improving the cleaning effect.

[0062] 4. Drying and subsequent processing

[0063] After cleaning, the heating stage 1.2 is reset, and the heating film 1.3 is heated to 50-60°C to quickly dry the slide 1.1. Then, the next round of staining can be carried out or the slide 1.1 can be removed for observation.

[0064] Through the above-described structural design and workflow, this invention significantly improves the efficiency and reliability of immunohistochemical staining, and is particularly suitable for time-sensitive scenarios such as intraoperative frozen sections.

[0065] The specific embodiments of this utility model have been described above, but the scope of protection of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the scope of protection of this utility model. Therefore, the scope of protection of this utility model should be determined by the scope of the claims.

[0066] In the description of this utility model, it should be understood that the terms "upper," "lower," "inner," "outer," etc., indicating the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. The word "comprising" does not exclude the presence of elements or steps not listed in the claims. The word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements. The simple fact that certain measures are recited in mutually different dependent claims does not indicate that a combination of these measures cannot be used for improvement. Any reference signs in the claims should not be construed as limiting the scope.

Claims

1. A glass slide holder for shaking and mixing, characterized in that, Including the slide holder body, it also includes: A glass slide, one side of which is provided with a groove for placing a sample and serving as a staining reaction area; A heating stage is mounted on the main body, and one side of the heating stage is connected to the main body via a pivot. The glass slide is placed on the heating stage. A rotating assembly is used to drive the heating platform to rotate around the rotating shaft at different angles; A drive component is used to drive the body to move horizontally.

2. The slide holder for shaking and mixing according to claim 1, characterized in that, The heating platform is provided with a mounting groove for placing the glass slide, and the two sides of the heating platform are also provided with guide grooves with a certain inclination angle.

3. The slide holder for shaking and mixing according to claim 2, characterized in that, The heating platform is also provided with a pair of columns, which are located on both sides of the mounting groove.

4. The slide holder for shaking and mixing according to claim 1, characterized in that, It also includes a heating film disposed at the bottom of the heating platform, on which a thermistor is disposed.

5. The slide holder for shaking and mixing according to claim 1, characterized in that, The heating platform is also equipped with a diffuse reflection sensor.

6. The slide holder for shaking and mixing according to claim 1, characterized in that, The rotating assembly includes one of an electric cylinder, a telescopic cylinder, or a rotary motor.

7. The slide holder for shaking and mixing according to claim 1, characterized in that, The rotating component is configured as an electric cylinder, with one end of the electric cylinder fixed to the base of the main body and the other end fixed to the base of the heating platform.

8. The slide holder for shaking and mixing according to claim 1, characterized in that, The drive assembly includes a motor, a synchronous belt, and a synchronous pulley. The main body is mounted on the synchronous belt, and the motor is connected to the synchronous pulley via the synchronous belt. The motor drives the synchronous belt and the main body to move horizontally back and forth.

9. The slide holder for shaking and mixing according to claim 2, characterized in that, It also includes a waste liquid collection platform located below the guide channel, with one end of the guide channel facing the waste liquid collection platform.

Images