A comprehensive test sample bin for automating phototweezing experiments

Abstract

A kind of comprehensive test sample bin for automating optical tweezer experiment, it relates to the field of optical tweezer micro-nano operation.The utility model discloses to solve the problem that existing ideal sample bin cannot comprehensively adjust flow field and temperature to simulate automated optical tweezer equipment.The utility model discloses that the inlet pipeline (1), the outlet pipeline (2) and the heating and temperature measuring device (3) are vertically installed on the sample bin base (4), for realizing the disturbance of convection field and time-varying temperature, the first cover glass (5) and the second cover glass (6) are capped in the middle of the upper end and the lower end of the sample bin base (4).The utility model can adjust flow field and temperature in time according to actual needs to simulate the final use environment of automated optical tweezer equipment, reduce the debugging cost in later period, improve the automation optical tweezer algorithm development efficiency, improve the operation level of automated optical tweezer equipment.The utility model is used for optical tweezer experiment.

Description

Technical Field

[0001] This utility model relates to a comprehensive testing sample chamber, specifically a comprehensive testing sample chamber for automated optical tweezers experiments. It is a device that uses the gradient force of laser focusing to control transparent micro / nano particles and cells, primarily applied in cell science research and micro / nano 3D printing research. It belongs to the field of optical tweezers micro / nano manipulation technology. Background Technology

[0002] Optical tweezers are devices that use the gradient force of a focused laser to control transparent micro / nano particles and cells. They are currently widely used in cell science research, micro / nano 3D printing, cell assembly, and other fields. With the large-scale application of optical tweezers, automated optical tweezers have gradually emerged, enabling large-scale, contactless manipulation at the micro / nano scale.

[0003] Automated optical tweezers equipment used in applications such as cell 3D printing is subject to various disturbances, including flow field disturbances, temperature inhomogeneity disturbances, and time-varying parameters such as time-varying temperature. Therefore, different influencing factors may appear under different operating conditions, affecting the operational performance of the automated optical tweezers system.

[0004] To address this issue, numerous intelligent control algorithms have been developed and validated in an ideal sample chamber. However, this research method has serious shortcomings, specifically:

[0005] (1) The ideal sample chamber is not subject to various external disturbances. The control algorithm developed in the ideal, disturbance-free sample chamber may not be applicable to actual working conditions.

[0006] (2) The development of intelligent algorithms is based on assumptions and remains at the theoretical stage. The comprehensive interference situation and algorithm optimization are not fully considered, or the algorithm is designed to be too complex in order to be applicable to various interference situations.

[0007] To improve the accuracy of optical tweezers experiments, further debugging and development of the optical tweezers operating equipment are needed.

[0008] In summary, existing ideal sample chambers cannot comprehensively adjust the flow field and temperature to simulate automated optical tweezers equipment. Utility Model Content

[0009] The purpose of this invention is to address the problem that existing ideal sample chambers cannot comprehensively adjust the flow field and temperature to simulate automated optical tweezers equipment. Therefore, it provides a comprehensive test sample chamber for automated optical tweezers experiments.

[0010] The technical solution of this utility model is as follows: A comprehensive test sample chamber for automated optical tweezers experiments includes an inlet pipe, an outlet pipe, a heating element and a thermometer, a sample chamber base, a first cover glass and a second cover glass. The inlet pipe, the outlet pipe, the heating element and the thermometer are vertically installed on the sample chamber base to achieve disturbance of the flow field and time-varying temperature. The first cover glass and the second cover glass cover the middle of the upper and lower ends of the sample chamber base.

[0011] Furthermore, the incoming flow pipeline includes an incoming stainless steel pipe and an incoming flexible hose, which are connected sequentially from bottom to top and then inserted into the sample chamber base.

[0012] Preferably, there is a nested connection between the incoming stainless steel pipe and the incoming flexible hose.

[0013] Furthermore, the outflow pipeline includes a stainless steel outflow pipe and a flexible outflow hose, which are connected sequentially from bottom to top and then inserted into the sample chamber base.

[0014] Preferably, there is a nested connection between the drain stainless steel pipe and the drain hose.

[0015] Furthermore, the sample chamber base is a cylindrical base.

[0016] Furthermore, an illumination light path hole is provided at the center of the lower end face of the sample chamber base, and a skirt is provided on the illumination light path hole with textured edges. The second cover glass is attached to the illumination light path hole.

[0017] Furthermore, a laser light path hole is machined at the center of the upper end face of the sample chamber base. A skirt is provided on the laser light path hole, and textures are machined on the skirt. The first cover glass is attached to the laser light path hole, and the experimental area is between the laser light path hole and the illumination light path hole.

[0018] Furthermore, the upper surface of the sample chamber base is machined with inlet pipe mounting holes, outlet pipe mounting holes, and temperature control mounting holes. The inlet pipe mounting holes, outlet pipe mounting holes, and temperature control mounting holes are installed on the platform outside the laser optical path hole. The inlet pipe, outlet pipe, heating and temperature measuring device are respectively installed on the inlet pipe mounting holes, outlet pipe mounting holes, and temperature control mounting holes.

[0019] Preferably, the inlet pipe mounting hole and the outlet pipe mounting hole are respectively provided with a platform edge, and the platform edge is connected to the experimental area.

[0020] Compared with the prior art, the present invention has the following advantages:

[0021] 1. This invention features a wide range of adjustable turbulence and a more uniform flow field. For the simulated flow field disturbance, the inlet and outlet pipes are connected to external pumps respectively. Precise control of the pumping speed of the two pumps ensures accurate simulation of the flow field disturbance, and the adjustable turbulence range is large. This provides more realistic experimental data and meets the specific needs of intelligent algorithm development.

[0022] 2. The present invention has a simple structure: the entire structure is contained in a standard 1-inch cylinder, which can be easily held by a standard optical clamp, making installation convenient and facilitating experiments.

[0023] 3. This invention boasts high overall performance: The effects of flow field and temperature can be verified through a comprehensive test sample chamber, providing a more accurate simulation of combined flow field and temperature disturbances. It overcomes the limitation of intelligent algorithm development remaining at the theoretical stage. The sample chamber of this invention allows for a more comprehensive consideration of overall disturbances and algorithm optimization, thus facilitating algorithm refinement. Attached Figure Description

[0024] Figure 1 This is an isometric drawing of this utility model;

[0025] Figure 2 This is the front view of this utility model;

[0026] Figure 3 This is a front sectional view of the present invention;

[0027] Figure 4 This is a bottom view of the present invention;

[0028] Figure 5 This is a side sectional view of the present invention;

[0029] Figure 6 This is a top view of the present invention.

[0030] In the diagram: 1. Inlet pipe, 1-1. Inlet stainless steel pipe, 1-2. Inlet flexible hose, 2. Outlet pipe, 2-1. Outlet stainless steel pipe, 2-2. Outlet flexible hose, 3. Heating and thermometer, 4. Sample chamber base, 4-1. Illumination light path hole, 4-2. Laser light path hole, 4-3. Inlet pipe mounting hole, 4-4. Outlet pipe mounting hole, 4-5. Temperature control mounting hole, 4-6. Stage edge, 5. First cover glass, 6. Second cover glass. Detailed Implementation

[0031] Specific implementation method one: Combining Figures 1 to 6This embodiment describes a comprehensive test sample chamber for automated optical tweezers experiments, comprising an inlet pipe 1, an outlet pipe 2, a heating element and a thermometer 3, a sample chamber base 4, a first coverslip 5, and a second coverslip 6.

[0032] The inlet pipe 1, the outlet pipe 2, and the heating and thermometer 3 are vertically installed on the sample chamber base 4 to achieve disturbance of the flow field and time-varying temperature. The first cover glass 5 and the second cover glass 6 are sealed on the middle of the upper and lower ends of the sample chamber base 4.

[0033] In this embodiment, both the first cover glass 5 and the second cover glass 6 are high-transmittance glass, which makes it easy to ensure that the light source can pass through.

[0034] This embodiment involves a comprehensive optical tweezers test sample chamber consisting of an incoming flow pipeline, an outgoing flow pipeline, a heating element, a thermometer, and a sample chamber base. The flow field and temperature can be adjusted in a timely manner as needed to simulate the final use environment of the automated optical tweezers equipment, reduce the cost of later debugging, improve the efficiency of automated optical tweezers algorithm development, and enhance the operational level of the automated optical tweezers equipment.

[0035] In this embodiment, the heating and temperature measuring device 3 is equipped with a resistance thermocouple and a thermocouple. The front end is designed as a column. By adjusting the whole, the temperature of the columnar stainless steel part can be controlled to simulate temperature disturbance in the test sample chamber.

[0036] Specific Implementation Method Two: Combining Figures 1 to 3 This embodiment describes the inflow pipeline 1, which includes an inflow stainless steel pipe 1-1 and an inflow flexible hose 1-2. The inflow stainless steel pipe 1-1 and the inflow flexible hose 1-2 are connected sequentially from bottom to top and then inserted into the sample chamber base 4.

[0037] With this configuration, the incoming stainless steel pipe 1-1 is connected to the sample chamber base 4, and the incoming flexible hose 1-2 is connected to an external pump body to provide turbulence for the experiment. Other components and connections are the same as in Specific Implementation Method 1.

[0038] Specific implementation method three: Combining Figures 1 to 3 This embodiment describes the nested connection between the incoming stainless steel pipe 1-1 and the incoming flexible hose 1-2. This arrangement allows the flexible hose 1-2 to connect to one end of the incoming stainless steel pipe 1-1 via its elasticity. For environments with high pump pressure, a clamping device is used for further connection. Other components and connections are the same as in specific embodiments one or two.

[0039] In this embodiment, the inner diameter of the incoming flow hose 1-2 is slightly larger than the outer diameter of the incoming flow stainless steel pipe 1-1, allowing for nested connections. Fluid is supplied into the incoming flow pipe to control the fluid disturbance within the sample chamber of the optical tweezers integrated testing system.

[0040] Specific implementation method four: Combination Figures 1 to 3 This embodiment describes a flow-out pipeline 2 comprising a stainless steel flow-out pipe 2-1 and a flexible flow-out hose 2-2, which are connected sequentially from bottom to top and then inserted into the sample chamber base 4. This arrangement facilitates control of the flow-out portion during the experiment and works in conjunction with the flow-in portion. Other components and connections are the same as in specific embodiments one, two, or three.

[0041] Specific Implementation Method Five: Combining Figures 1 to 3 This embodiment describes the nested connection between the outflow stainless steel pipe 2-1 and the outflow hose 2-2. This configuration allows fluid to be delivered into the outflow pipe, controlling the outflow portion and minimizing fluid disturbance within the optical tweezers integrated testing sample chamber. Other components and connections are the same as in specific embodiments one, two, three, or four.

[0042] In this embodiment, the inner diameter of the drain hose 2-2 is slightly larger than the outer diameter of the drain stainless steel pipe 2-1, and they can be nested together.

[0043] Specific Implementation Method Six: Combination Figures 1 to 6 In this embodiment, the sample chamber base 4 is a cylindrical base. This design results in a simple and compact structure, facilitating experimentation with standard optical clamps. Other components and connections are the same as in specific embodiments one, two, three, four, or five.

[0044] Specific implementation method seven: Combining Figure 3 and Figure 5 In this embodiment, the sample chamber base 4 has an illumination light path hole 4-1 at the center of its lower end face. The illumination light path hole 4-1 has a skirt with textured edges. The second cover glass 6 is attached to the illumination light path hole 4-1.

[0045] This setup provides light transmission conditions for the experiment. Other components and connections are the same as in specific implementation methods one, two, three, four, five, or six.

[0046] Specific implementation method eight: Combination Figure 3 and Figure 5 In this embodiment, the upper surface of the sample chamber base 4 is machined with a laser light path hole 4-2. The laser light path hole 4-2 is provided with a skirt, and the skirt is machined with texture. The first cover glass 5 is attached to the laser light path hole 4-2. The area between the laser light path hole 4-2 and the illumination light path hole 4-1 is the experimental area.

[0047] This setup provides a region through which the laser can pass in the experiment. Other components and connections are the same as in any of the specific embodiments one through seven.

[0048] In this embodiment, there is still a cavity between the laser light path hole 4-2 and the illumination light path hole 4-1, which is used to place experimental items such as cells.

[0049] Specific Implementation Method Nine: Combining Figure 3 and Figure 5 In this embodiment, the upper surface of the sample chamber base 4 is machined with an inlet pipe mounting hole 4-3, an outlet pipe mounting hole 4-4, and a temperature control mounting hole 4-5. The inlet pipe mounting hole 4-3, the outlet pipe mounting hole 4-4, and the temperature control mounting hole 4-5 are mounted on a platform outside the laser optical path hole 4-2. The inlet pipe 1, the outlet pipe 2, and the heating and temperature measuring device 3 are respectively mounted on the inlet pipe mounting hole 4-3, the outlet pipe mounting hole 4-4, and the temperature control mounting hole 4-5.

[0050] With this configuration, the inlet pipe mounting hole 4-3, the outlet pipe mounting hole 4-4, and the temperature control mounting hole 4-5 are used to install the inlet pipe 1, the outlet pipe 2, the heating element, and the temperature sensor 3, thus simulating the actual experimental environment through the inlet, outlet, and temperature control methods. Other components and connections are the same as in any of the specific implementation methods one through eight.

[0051] Specific Implementation Method Ten: Combining Figure 3 In this embodiment, the inlet pipe mounting hole 4-3 and the outlet pipe mounting hole 4-4 are respectively provided with a platform 4-6, and the platform 4-6 is connected to the experimental area.

[0052] With this configuration, the rim 4-6 can effectively prevent pipe insertion from becoming blocked. Other components and connections are the same as in any of the specific implementation methods one through eight.

[0053] In this embodiment, the inlet pipe mounting hole 4-3 is provided with three small holes that flow out from the side to ensure smooth flow; the last hole is used for temperature adjustment and compensation, and there is a platform at the bottom for limiting.

[0054] Combination Figures 1 to 6 This indicates that it can be specifically applied to the research and development stage of optical tweezers equipment.

[0055] Installation Stage: First, attach the first coverslip 5 and the second coverslip 6 to the sample chamber base 4 using sealant. The fine lines on the skirt of the sample chamber base 4 ensure a firm bond. Next, use a standard 4mm stainless steel pipe and a 4.15mm inner diameter flexible hose, consisting of inlet pipe 1 and outlet pipe 2, and insert them into the limiting positions of the sample chamber base 4, securing them with sealant. Then, insert a 5.5mm outer diameter heating and thermometer 3 into the corresponding holes of the sample chamber base 4 and limit its position, finally bonding them with sealant.

[0056] Usage phase: According to the required simulated working conditions and solution environment, connect the pump body to the back hose of the inlet pipe 1 or outlet pipe 2 to achieve precise control of the simulated disturbance flow field. By controlling the closed loop of current and temperature feedback of heating and thermometer 3, the temperature disturbance of the comprehensive test sample chamber can be precisely controlled.

[0057] At this time, we will carry out research and development of control algorithms for optical tweezers equipment, evaluate the robustness of the algorithm under this disturbance, and then develop high-performance optical tweezers control algorithms to improve the accuracy of automated optical tweezers equipment.

[0058] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Those skilled in the art can make other changes within the spirit of the present invention and apply it to fields not mentioned in the present invention. Of course, all such changes made in accordance with the spirit of the present invention should be included within the scope of protection claimed by the present invention.

Claims

1. A comprehensive test sample chamber for automated optical tweezers experiments, characterized in that: It includes an inlet pipe (1), an outlet pipe (2), a heating element and a thermometer (3), a sample chamber base (4), a first cover glass (5), and a second cover glass (6). The inlet pipe (1), outlet pipe (2) and heating and thermometer (3) are vertically installed on the sample chamber base (4) to achieve disturbance of the flow field and time-varying temperature. The first cover glass (5) and the second cover glass (6) are sealed on the middle of the upper and lower ends of the sample chamber base (4).

2. The comprehensive test sample chamber for automated optical tweezers experiments according to claim 1, characterized in that: The incoming flow pipeline (1) includes an incoming stainless steel pipe (1-1) and an incoming flexible hose (1-2). The incoming stainless steel pipe (1-1) and the incoming flexible hose (1-2) are connected from bottom to top and then inserted into the sample chamber base (4).

3. The comprehensive test sample chamber for automated optical tweezers experiments according to claim 2, characterized in that: Nested connection between the incoming stainless steel pipe (1-1) and the incoming flexible hose (1-2).

4. The comprehensive test sample chamber for automated optical tweezers experiments according to claim 3, characterized in that: The outflow pipeline (2) includes an outflow stainless steel pipe (2-1) and an outflow hose (2-2). The outflow stainless steel pipe (2-1) and the outflow hose (2-2) are connected from bottom to top and then inserted into the sample chamber base (4).

5. A comprehensive test sample chamber for automated optical tweezers experiments according to claim 4, characterized in that: Nested connection between the stainless steel drain pipe (2-1) and the drain hose (2-2).

6. The comprehensive test sample chamber for automated optical tweezers experiments according to claim 5, characterized in that: The sample chamber base (4) is a cylindrical base.

7. A comprehensive test sample chamber for automated optical tweezers experiments according to claim 6, characterized in that: The sample chamber base (4) has an illumination light path hole (4-1) at the center of its lower end face. The illumination light path hole (4-1) has a skirt with textured edges. The second cover glass (6) is attached to the illumination light path hole (4-1).

8. A comprehensive test sample chamber for automated optical tweezers experiments according to claim 7, characterized in that: The upper end face of the sample chamber base (4) is machined with a laser light path hole (4-2). The laser light path hole (4-2) is provided with a skirt with texture. The first cover glass (5) is pasted on the laser light path hole (4-2). The experimental area is between the laser light path hole (4-2) and the illumination light path hole (4-1).

9. A comprehensive test sample chamber for automated optical tweezers experiments according to claim 8, characterized in that: The upper surface of the sample chamber base (4) is machined with inlet pipe mounting holes (4-3), outlet pipe mounting holes (4-4) and temperature control mounting holes (4-5). The inlet pipe mounting holes (4-3), outlet pipe mounting holes (4-4) and temperature control mounting holes (4-5) are installed on the platform outside the laser optical path hole (4-2). The inlet pipe (1), outlet pipe (2) and heating and temperature measuring device (3) are respectively installed on the inlet pipe mounting holes (4-3), outlet pipe mounting holes (4-4) and temperature control mounting holes (4-5).

10. A comprehensive test sample chamber for automated optical tweezers experiments according to claim 9, characterized in that: An edge (4-6) is provided on the inlet pipe mounting hole (4-3) and the outlet pipe mounting hole (4-4), and the edge (4-6) is connected to the experimental area.

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