Buckwheat living plant leaf inoculation device

Abstract

The utility model belongs to the plant inoculation technical field, specifically disclose buckwheat living plant leaf inoculation device, including the pen container, the pen container lower part fixed installation conical puncher, the pen container top installation puncture needle, the puncher inside is provided with the piston, the piston is connected with the plug rod upwards, the plug rod runs through puncher top and is connected with the plug head, and the plug rod outside sets up the spring support between puncher and plug head, the pen container side wall is seted up with the sliding slot, the plug head side wall is connected to the outward connection and is connected to the operation handle of sliding slot. When inoculating, first use puncture needle to prick the tiny hole on buckwheat leaf, then switch the puncher at the bottom of pen container to cut the inoculation cake on the pathogenic bacteria culture medium flat plate, pull down the operation handle before cutting, make the piston slide down in the puncher, take out the cake through the puncher, loosen the operation handle, produce the negative pressure suction force in the puncher, and the cake can be transferred and inoculated on the buckwheat leaf. The whole inoculation process is continuous and fast, and the inoculation efficiency is high.

Description

Technical Field

[0001] This utility model belongs to the field of plant inoculation technology, specifically relating to a device for inoculating the leaves of live buckwheat plants. Background Technology

[0002] Buckwheat is used as a model plant in agricultural research and is used in plant disease resistance breeding research. The inoculation experiment of its live plants is of great significance for exploring the disease resistance mechanism of buckwheat and cultivating superior varieties.

[0003] The inoculation procedure for live buckwheat plants involved the following steps: First, researchers used a puncture needle to create tiny holes (0.1 mm-0.2 mm) in the buckwheat leaves. Then, a perforator was used to create holes in the pathogen culture medium plate to collect mycelial cakes. These mycelial cakes, each 6 mm in diameter, were then collected and inoculated onto the tiny holes in the buckwheat leaves using an inoculation needle. This inoculation process required the preparation of multiple tools and frequent tool changes, increasing the complexity and reducing efficiency. Utility Model Content

[0004] To solve the above-mentioned technical problems, this utility model designs a buckwheat live plant leaf inoculation device to facilitate buckwheat live plant inoculation operations and improve inoculation efficiency.

[0005] The present invention relates to a buckwheat live plant leaf inoculation device, comprising a pen holder, a conical punch fixedly installed at the lower part of the pen holder, and a puncture needle installed at the top of the pen holder; a piston is provided inside the punch, and a stopper rod is connected upward to the piston; the stopper rod passes through the top of the punch and is connected to the stopper head, and a spring is provided outside the stopper rod to support it between the punch and the stopper head; a sliding groove is provided on the side wall of the pen holder, and an operating handle that extends outward through the sliding groove is connected to the side wall of the stopper head.

[0006] The top of the pen holder is also threaded with a pen cap.

[0007] The pen cap is provided with anti-slip texture.

[0008] The plug has several sliders that are equidistantly arranged to engage with the grooves opened on the inner wall of the pen holder.

[0009] The beneficial effects of this utility model are:

[0010] Compared with existing technologies, this invention integrates the punch and puncture needle into one unit. During inoculation, the puncture needle at the top of the pen holder first punctures a 0.1 mm-0.2 mm micro-hole on the buckwheat leaf. Then, the punch at the bottom of the pen holder is used to cut the inoculation cake from the pathogen culture medium plate. Before cutting, the operating handle is pulled down, causing the stopper to slide down and push the piston down inside the punch. A 6 mm diameter mycelium cake is then removed from the pathogen culture medium plate through the punch end. After the mycelium cake is cut, the operating handle is released, and the stopper slides down under the return action of the spring. At the same time, the piston generates a negative pressure suction force inside the punch, adsorbing the mycelium cake at the punch opening, thus transferring the mycelium cake to the micro-hole punctured on the buckwheat leaf. The entire inoculation process can be completed continuously and quickly using this inoculation device, which is convenient to operate and has high inoculation efficiency. Attached Figure Description

[0011] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments are briefly introduced below.

[0012] Figure 1 This is a schematic diagram of the overall structure of the inoculation device in the embodiment.

[0013] Figure 2 This is an internal cross-sectional view of the inoculation device in the embodiment.

[0014] Figure 3 This is a schematic diagram of the structure of the inoculation device compared to the top of the pen holder in the embodiment.

[0015] In the attached diagram, the structural names represented by each number are as follows:

[0016] 1-Pen holder, 2-Punch man, 3-Punch needle, 4-Pen cap, 5-Piston, 6-Plug rod, 7-Plug head, 8-Spring, 9-Slider, 10-Groove, 11-Operating handle, 12-Anti-slip texture. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0018] Example 1

[0019] To facilitate the inoculation of live buckwheat plants and improve the efficiency of the inoculation process, please refer to... Figures 1 to 3As shown, the buckwheat live plant leaf inoculation device in this embodiment is shaped like a pen holder 1. A conical punch 2 is fixedly installed at the lower part of the pen holder 1. The punch 2 is used to cut the mycelium cake. A piercing needle 3 is integrated at the top of the pen holder 1. The piercing needle 3 is used to puncture tiny holes in the buckwheat leaves for inoculation. A piston 5 is provided inside the punch 2. A stopper rod 6 is connected upward to the piston 5. The stopper rod 6 passes through the top of the punch 2 and is connected to the stopper head 7. A spring 8 is provided outside the stopper rod 6 to support it between the punch 2 and the stopper head 7. In the initial state, the piston 5 is close to the inner top surface of the punch 2. In order to facilitate the control of the movement of the piston 5, a groove 10 is opened on the side wall of the pen holder 1. An operating handle 11 that extends outward through the groove 10 is connected to the side wall of the stopper head 7.

[0020] During inoculation, the operator holds the pen holder 1 and uses the puncture needle 3 at the top to lightly prick the buckwheat leaf, creating tiny holes of 0.1 mm-0.2 mm to provide inoculation sites for the mycelium cake. Then, the pen holder 1 is flipped so that the perforator 2 at the bottom is aligned with the pathogen culture medium plate. The operating handle 11 is pulled down, causing the stopper 7 to push the piston 5 down through the stopper rod 6. At this point, the conical end of the perforator 2 cuts into the pathogen culture medium plate, removing a mycelium cake with a diameter of 6 mm. The operating handle 11 is then released, the spring 8 returns to its original position, and the stopper 7 slides upward. The piston 5 creates a negative pressure suction force within the perforator 2, adsorbing the cut mycelium cake onto the port of the perforator 2, thus achieving stable mycelium cake gripping. Finally, the port of the perforator 2 with the adsorbed mycelium cake is aligned with the tiny holes punctured on the leaf, and the device is gently pressed to ensure the mycelium cake adheres to the hole, completing the inoculation. The entire inoculation process can be completed continuously and quickly using only this inoculation device, eliminating the need for frequent tool changes, making it convenient to operate and greatly improving inoculation efficiency.

[0021] In practice, it has been verified that the stability of bacterial strains captured by negative pressure suction adsorption is much higher than that of bacterial strains inoculated by needle picking, while also greatly improving efficiency.

[0022] To prevent the puncture needle 3 from accidentally injuring the operator, a pen cap 4 is threadedly connected to the top of the pen holder 1. The pen cap 4 is provided with anti-slip texture 12 to increase friction and make it easier to screw the pen cap 4.

[0023] To further improve the stability of the stopper 7, especially the stability of the stopper 7 driving the piston 5 to slide during the spring 8's rebound, several sliders 9 are equidistantly arranged on the stopper 7 to engage with the grooves 10 opened on the inner wall of the pen holder 1. The contact between the sliders 9 and the grooves 10 on the inner wall of the pen holder 1 increases the friction, making the sliding of the stopper 7 driving the piston 5 more stable.

[0024] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not describe all details exhaustively, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification.

Claims

1. A buckwheat live plant leaf inoculation device, characterized in that, The device includes a pen holder (1), a conical punch (2) is fixedly installed at the lower part of the pen holder (1), and a puncture needle (3) is installed at the top of the pen holder (1); a piston (5) is provided inside the punch (2), and a stopper rod (6) is connected upward to the piston (5). The stopper rod (6) passes through the top of the punch (2) and is connected to the stopper head (7). A spring (8) is provided outside the stopper rod (6) to support it between the punch (2) and the stopper head (7). A sliding groove (10) is provided on the side wall of the pen holder (1), and an operating handle (11) that passes out of the sliding groove (10) is connected to the side wall of the stopper head (7).

2. The buckwheat live plant leaf inoculation device according to claim 1, characterized in that, The top of the pen holder (1) is also threaded with a pen cap (4).

3. The buckwheat live plant leaf inoculation device according to claim 2, characterized in that, The pen cap (4) is provided with anti-slip texture (12).

4. The buckwheat live plant leaf inoculation device according to claim 1, characterized in that, The plug (7) is provided with several sliders (9) at equal intervals, which are engaged with the grooves (10) opened on the inner wall of the pen holder (1).

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