Edible mushroom sterilization, cooling and inoculation integrated chamber based on normal pressure steam sterilization mode
By designing an integrated sterilization, cooling, and inoculation chamber for edible fungi under normal pressure steam sterilization, the problem of contamination of mushroom bags under normal pressure steam sterilization was solved, achieving a clean environment for sterilization, cooling, and inoculation, thus improving the success rate of mushroom bag inoculation and the safety of staff.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHAOGUAN COLLEGE
- Filing Date
- 2025-07-25
- Publication Date
- 2026-06-23
AI Technical Summary
When sterilizing and inoculating using atmospheric pressure steam in an open environment, it can easily lead to contamination of the spawn bags, especially in seasons with high temperature and humidity. An unclean inoculation environment can affect the success rate of the spawn bags.
Design an integrated sterilization, cooling, and inoculation room for edible fungi based on atmospheric pressure steam sterilization. It includes a shoe-changing room, a changing room, a buffer room, and multiple sterilization, cooling, and inoculation areas. It is equipped with ultraviolet lamps, fan filter units, ozone generators, and other equipment to form a clean area, maintain the cleanliness of the sterilization, cooling, and inoculation environment, and avoid contamination of the mushroom bags.
The integrated design reduces the handling of mushroom bags, improves the success rate of inoculation, avoids environmental pollution, ensures a clean inoculation environment, and reduces the health risks for inoculation staff.
Smart Images

Figure CN224386362U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of edible fungi cultivation technology, and more specifically, it relates to an integrated sterilization, cooling and inoculation chamber for edible fungi based on atmospheric pressure steam sterilization. Background Technology
[0002] There are two main methods for sterilizing edible mushroom culture media: high-pressure steam sterilization and atmospheric-pressure steam sterilization. High-pressure steam sterilization is typically used in large-scale industrialized edible mushroom production enterprises, such as those producing enoki mushrooms and king oyster mushrooms. Atmospheric-pressure steam sterilization is mainly used by small and medium-sized enterprises for producing seasonal edible mushroom varieties, such as oyster mushrooms, wood ear mushrooms, and shiitake mushrooms. Compared to high-pressure steam sterilization, atmospheric-pressure steam sterilization facilities are simpler, have lower construction costs, are easier to operate, and are more widely used.
[0003] Atmospheric pressure steam sterilization chambers are typically located in open outdoor environments. After sterilization, the spawn bags are either cooled in situ, transferred to an inoculation room, or separated into separate cooling and inoculation rooms. Regardless of whether the spawn bags are cooled in a separate cooling room or in an inoculation room, the transfer of spawn bags is involved. Traditional atmospheric pressure steam sterilization methods often lack sterile rooms, and the disinfection of inoculation staff is too simplistic, sometimes even neglecting to change into cleanroom suits. In such cases, the inoculation environment is easily contaminated by bacteria introduced by the staff, polluting the air. To avoid infection during the inoculation process, spawn bag production is often scheduled during the cooler, drier autumn and winter months, when the activity of bacteria in the natural environment is lower, reducing the risk of contamination after inoculation. However, in the spring and summer months, with rising temperatures and higher humidity, this open inoculation method easily leads to widespread bacterial contamination of the spawn bags, resulting in cultivation failure.
[0004] Therefore, it is necessary to propose an integrated chamber for sterilization, cooling, and inoculation of edible fungi based on atmospheric pressure steam sterilization to solve the above problems. Summary of the Invention
[0005] To address the shortcomings of existing technologies, the purpose of this invention is to provide an integrated sterilization, cooling, and inoculation room for edible fungi based on atmospheric pressure steam sterilization. This room maintains a clean inoculation environment in the sterilization, cooling, and inoculation areas, ensuring a high success rate for inoculating mushroom bags. It replaces the existing separate sterilization room, cooling room, and inoculation room, reducing the handling of mushroom bags and avoiding contamination issues caused by insufficiently clean air during handling.
[0006] The above-mentioned technical objective of this utility model is achieved through the following technical solution:
[0007] An integrated sterilization, cooling and inoculation room for edible fungi based on atmospheric pressure steam sterilization includes a shoe changing room, a changing room, a buffer room and multiple sterilization, cooling and inoculation areas. The shoe changing room is connected to the changing room, the changing room is connected to the buffer room, and the buffer room is connected to the multiple sterilization, cooling and inoculation areas.
[0008] The shoe changing room, changing room and buffer room are all equipped with ultraviolet lamps. The changing room and buffer room are equipped with fan filter units and three-stage filtration fresh air units. The buffer room is equipped with an ozone generator. Along the length of the buffer room, the ozone generator and the three-stage filtration fresh air unit are respectively located at both ends of the buffer room.
[0009] There is a separation area between the sterilization and cooling inoculation area and the buffer room. The sterilization and cooling inoculation area is equipped with a heat insulation wall on the side facing the buffer room. The ozone generator is connected to multiple sterilization and cooling inoculation areas through pipes. The sterilization and cooling inoculation area is equipped with a three-stage filtration fresh air unit. LED light strips are installed at the location where the buffer room connects to the sterilization and cooling inoculation area.
[0010] In one embodiment, the sterilization cooling inoculation area includes a frame, a film, and an insulation layer. The frame is welded from a high-temperature resistant and rust-proof material, and the frame is covered with a film and an insulation layer.
[0011] In one embodiment, the number of sterilization and cooling inoculation zones is three.
[0012] In one embodiment, the distance between the sterilization and cooling inoculation area and the buffer chamber is ≥20 cm.
[0013] In one embodiment, a pass-through window is provided between the shoe changing room and the changing room, and a pass-through window is also provided between the changing room and the buffer room.
[0014] In one embodiment, the transfer window is equipped with an ultraviolet disinfection lamp.
[0015] In one embodiment, the shoe changing room is equipped with a disinfection cabinet, shoe racks, coat racks and handwashing sinks.
[0016] In one embodiment, the changing room is equipped with cleanroom wardrobes.
[0017] In summary, this utility model has the following beneficial effects:
[0018] This invention combines a shoe-changing room, a changing room, and a buffer room into a clean area. Simultaneously, it filters and controls the air entering the sterilization, cooling, and inoculation area to maintain a clean inoculation environment and ensure a high success rate for inoculation. This invention places sterilization, cooling, and inoculation in the same area, forming an "integrated room," replacing the existing separate sterilization, cooling, and inoculation rooms. This reduces the workload of transporting mushroom bags and avoids contamination issues caused by insufficiently clean air during transport. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the present invention.
[0020] In the diagram: 1. Shoe changing room, 2. Changing room, 3. Buffer room, 4. Sterilization, cooling and inoculation area, 5. Ultraviolet lamp, 6. Fan and filter unit, 7. Three-stage filtration fresh air unit, 8. Ozone generator, 9. Insulated wall, 10. Pass-through window, 11. Shoe rack, 12. Clothes rack, 13. Cleanroom garment wardrobe, 14. LED light strip. Detailed Implementation
[0021] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.
[0022] It is worth noting that the directional terms such as "up" and "down" used in this article are all relative to the perspective of the attached figures and are only for the purpose of description. They should not be interpreted as limitations on the technical solutions.
[0023] like Figure 1 As shown, this utility model proposes an integrated sterilization, cooling and inoculation room for edible fungi based on atmospheric pressure steam sterilization, including a shoe changing room 1, a changing room 2, a buffer room 3 and multiple sterilization, cooling and inoculation areas 4. The shoe changing room 1 is connected to the changing room 2, the changing room 2 is connected to the buffer room 3, and the buffer room 3 is connected to the multiple sterilization, cooling and inoculation areas 4.
[0024] Ultraviolet lamps 5 are installed in shoe changing room 1, changing room 2 and buffer room 3. Fan filter unit 6 (FFU) and three-stage filter fresh air unit 7 are installed in changing room 2 and buffer room 3. Ozone generator 8 is installed in buffer room 3. Along the length of buffer room 3, ozone generator 8 and three-stage filter fresh air unit 7 are respectively installed at both ends of buffer room 3.
[0025] There is a separation area between the sterilization and cooling inoculation area 4 and the buffer room 3. The side of the sterilization and cooling inoculation area 4 facing the buffer room 3 is equipped with a heat insulation wall 9. The ozone generator 8 is connected to multiple sterilization and cooling inoculation areas 4 through pipes. The sterilization and cooling inoculation area 4 is equipped with a three-stage filtration fresh air unit 7. An LED light strip 14 is provided at the position where the buffer room 3 connects to the sterilization and cooling inoculation area 4.
[0026] In this invention, the shoe-changing room 1, changing room 2, and buffer room 3 can be constructed from warehouse panels to reduce construction costs. These rooms are routinely disinfected using ultraviolet lamps 5. The fan-filter unit 6 and the three-stage filtration fresh air unit 7 within changing room 2 and buffer room 3 are used to purify the air and maintain a positive pressure environment, preventing the entry of external bacteria. An ozone generator 8 installed in buffer room 3 generates ozone to sterilize and cool the inoculation area 4 and to disinfect the inoculum bags.
[0027] When staff enter changing room 2 from shoe changing room 1, the fan-filter unit 6 and the three-stage filtration fresh air unit 7 are turned on to purify the air and maintain a positive pressure environment in changing room 2, preventing air contamination from shoe changing room 1. Staff change into cleanroom garments in changing room 2, turn off the three-stage filtration fresh air unit 7, and then enter buffer room 3. When staff enter changing room 2 from shoe changing room 1, the fan-filter unit 6 and the three-stage filtration fresh air unit 7 in buffer room 3 are turned on to purify the air in buffer room 3 and maintain a positive pressure environment.
[0028] The sterilization and cooling inoculation area 4 serves the functions of sterilization, cooling, and inoculation. After the spawn bags in the sterilization and cooling inoculation area 4 have cooled down, the environment and spawn bags within the area need to be disinfected. The ozone generator 8 in the buffer chamber 3 is activated to generate ozone, which is then piped into the sterilization and cooling inoculation area 4 for disinfection. After disinfection, the three-stage filtered fresh air unit 7 in the sterilization and cooling inoculation area 4 introduces fresh air and removes residual ozone. Inoculation staff then guide LED light strips 14 into the sterilization and cooling inoculation area 4 to provide illumination, after which inoculation can proceed. During inoculation, the three-stage filtered fresh air unit 7 remains on, maintaining a positive pressure in the sterilization and cooling inoculation area 4 to prevent outside air from entering and contaminating the inoculation environment, thereby improving the success rate of spawn bag inoculation.
[0029] Under normal pressure steam sterilization, after sterilization and cooling, the inoculum bags will be fumigated with an aerosol disinfectant. After the disinfectant mist dissipates, the inoculation staff will then enter the sterilization room. The aerosol disinfectant is mainly sodium dichloroisocyanurate. Residual sodium dichloroisocyanurate in the inoculation environment can easily be directly contacted or inhaled by inoculation staff, potentially causing skin burns, redness, swelling, and itching, or irritating the respiratory tract, leading to coughing, sore throat, and difficulty breathing. Sodium dichloroisocyanurate disinfectant may also damage the respiratory mucosa, increasing the risk of chronic bronchitis. This invention effectively solves this problem.
[0030] The sterilization and cooling inoculation area 4 is separated from the buffer chamber 3 by a partition, and the sterilization and cooling inoculation area 4 is equipped with a heat insulation wall 9, which serves to insulate and prevent heat from the sterilization and cooling area from being transferred to the buffer chamber 3, causing the temperature of the buffer chamber 3 to rise. Preferably, the distance between the partition between the sterilization and cooling inoculation area and the buffer chamber 3 is ≥20 cm. In this invention, the frame of the sterilization and cooling inoculation area 4 is welded from a high-temperature resistant and rust-proof material such as stainless steel, and the frame is covered with a thin film and a heat insulation layer, for example, the heat insulation layer material is aluminum silicate fiber needled blanket.
[0031] As is easily understood, in this invention, connecting doors are provided between the shoe changing room 1 and the changing room 2, between the changing room 2 and the buffer room 3, and between the buffer room 3 and each sterilization and cooling inoculation area 4. This facilitates movement of inoculation operators between areas, allowing them to close the corresponding doors after entering or exiting each area. In this invention, the sterilization and cooling inoculation area 4 also includes conventional equipment such as a steam heating device and a cooling device, enabling it to perform normal sterilization and cooling functions.
[0032] like Figure 1 As shown, a pass-through window 10 is installed between shoe changing room 1 and changing room 2, and a pass-through window 10 is also installed between changing room 2 and buffer room 3. Edible fungi spawn is transferred through the pass-through window 10 to avoid environmental pollution caused by repeated entry and exit from different areas.
[0033] Furthermore, the transfer window 10 is equipped with ultraviolet disinfection lamps to disinfect the surface of the edible fungi strains and the space environment of the transfer window 10 with ultraviolet light.
[0034] like Figure 1 As shown, shoe changing room 1 is equipped with shoe rack 11, coat rack 12 and handwashing sink, which facilitates the vaccination operators to change shoes, take off coats and wash hands. Shoe changing room 1 is also equipped with a disinfection cabinet to store commonly used disinfection supplies, such as alcohol and chlorine dioxide disinfectant, for surface disinfection of edible fungi spawn.
[0035] like Figure 1 As shown, changing room 2 is equipped with cleanroom garment wardrobe 13 for storing cleanroom garments.
[0036] Preferably, there are three sterilization-cooling-inoculation zones 4 to achieve daily production rotation. For example, one sterilization-cooling-inoculation zone 4 is in the sterilization state, one sterilization-cooling-inoculation zone 4 is in the cooling state, and one sterilization-cooling-inoculation zone 4 is in the inoculation state.
[0037] The sterilization cooling inoculation area 4 of this invention is covered with a thin film and a layer of heat-insulating material, replacing the original canvas cover, which enhances the heat-insulating function of the sterilization cooling inoculation area 4, saves energy and increases efficiency, accelerates the steam heating process, and improves the sterilization effect.
[0038] This invention places sterilization, cooling, and inoculation in the same area, forming an "integrated room," which replaces the existing separate sterilization room, cooling room, and inoculation room. This reduces the work of transporting the mushroom bags and avoids the problem of mushroom bag contamination due to insufficient clean air during transportation.
[0039] Traditional atmospheric pressure steam sterilization methods typically lack a sterile room, and the disinfection of inoculation staff is often too simplistic, sometimes even neglecting to change into cleanroom suits. This makes the inoculation environment susceptible to contamination from the external environment and staff, leading to air pollution and increasing the contamination rate of the inoculated spawn. This invention comprises a clean area consisting of a shoe-changing room 1, a changing room 2, and a buffer room 3. Its purpose is to address the hygiene issues of inoculation staff and to filter and control the air entering the sterilization and cooling inoculation area 4, maintaining a clean inoculation environment and ensuring a high success rate for spawn inoculation.
[0040] In this utility model, the dimensions of the shoe changing room 1, changing room 2, buffer room 3, and sterilization and cooling inoculation area 4 are determined according to the design. For example, the shoe changing room 1 is 5 m long and 2.5 m wide; the changing room 2 is 5 m long and 1.5 m wide; the buffer room 3 is 11 m long and 2 m wide; the sterilization and cooling inoculation area 4 is 7 m long, 3 m wide, and 1.8 m high, and each sterilization and cooling inoculation area 4 is spaced 1 m apart.
[0041] In this invention, the air volume of the three-stage filtration fan is 1500 m³ / h. 3 ozone generator 8 produces 10g / h of ozone;
[0042] The door frame of the sterilization and cooling inoculation area 4 near the buffer chamber 3 is sealed with a high-temperature resistant silicone seal to prevent high-temperature steam from leaking into the buffer chamber 3 when the sterilization and cooling inoculation area 4 is in the sterilization state.
[0043] This invention relates to a disinfection method that uses ozone instead of aerosol disinfectant fumigation to achieve the goal of health and environmental protection. The sterilization and cooling inoculation area 4 is covered with an insulating material such as aluminum silicate fiber needled blanket, replacing the traditional canvas cover, which reduces energy loss during steam sterilization and improves the sterilization effect.
[0044] The method of using this invention is as follows: 0.5-1 hour before inoculation, turn on the ozone generator 8 to disinfect the sterilized and cooled inoculation area 4. 0.5 hours before inoculation, the inoculation staff treats the bacterial culture, disinfects it, and places it in the transfer window 10 between the shoe-changing room 1 and the changing room 2, then irradiates it with ultraviolet lamps 5 for 30 minutes. The inoculation staff changes shoes and removes their coats in the shoe-changing room 1, then enters the changing room 2, puts on cleanroom clothing, and transfers the bacterial culture to the transfer window 10 between the changing room 2 and the buffer room 3. Upon entering the buffer room 3, turn on the three-stage filtered fresh air unit 7 to deliver sterile air to the integrated inoculation room, expelling residual ozone, and guide the LED light strip 14 into the sterilized and cooled inoculation area 4 to provide illumination. Then, carrying the bacterial culture and tools, the staff enters the sterilized and cooled inoculation area 4 to begin the inoculation operation.
[0045] The above description is merely a preferred embodiment of this utility model. The protection scope of this utility model is not limited to the above embodiments. All technical solutions falling within the scope of this utility model's concept are protected. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principle of this utility model should also be considered within the protection scope of this utility model.
Claims
1. An integrated sterilization, cooling, and inoculation chamber for edible fungi based on atmospheric pressure steam sterilization, characterized in that: It includes a shoe changing room (1), a changing room (2), a buffer room (3) and multiple sterilization and cooling inoculation areas (4). The shoe changing room (1) is connected to the changing room (2), the changing room (2) is connected to the buffer room (3), and the buffer room (3) is connected to multiple sterilization and cooling inoculation areas (4). Ultraviolet lamps (5) are installed in shoe changing room (1), changing room (2) and buffer room (3). Fan filter unit (6) and three-stage filter fresh air fan (7) are installed in changing room (2) and buffer room (3). Ozone generator (8) is installed in buffer room (3). Along the length of buffer room (3), ozone generator (8) and three-stage filter fresh air fan (7) are respectively installed at both ends of buffer room (3). There is a gap between the sterilization cooling inoculation area (4) and the buffer room (3). The sterilization cooling inoculation area (4) is equipped with a heat insulation wall (9) on the side facing the buffer room (3). The ozone generator (8) is connected to multiple sterilization cooling inoculation areas (4) through pipes. The sterilization cooling inoculation area (4) is equipped with a three-stage filtration fresh air unit (7). An LED light strip (14) is provided at the position where the buffer room (3) connects to the sterilization cooling inoculation area (4).
2. The integrated sterilization, cooling, and inoculation chamber for edible fungi as described in claim 1, characterized in that, The sterilization and cooling inoculation area (4) includes a frame, a film and an insulation layer. The frame is welded from high-temperature resistant and rust-proof materials, and the frame is covered with a film and an insulation layer.
3. The integrated sterilization, cooling, and inoculation chamber for edible fungi as described in claim 1, characterized in that, The number of sterilization and cooling inoculation areas (4) is three.
4. The integrated sterilization, cooling, and inoculation chamber for edible fungi as described in claim 1, characterized in that, The distance between the sterilization and cooling inoculation area and the buffer room (3) is ≥20 cm.
5. The integrated sterilization, cooling, and inoculation chamber for edible fungi as described in any one of claims 1-4, characterized in that, A pass-through window (10) is provided between the shoe changing room (1) and the changing room (2), and a pass-through window (10) is also provided between the changing room (2) and the buffer room (3).
6. The integrated sterilization, cooling, and inoculation chamber for edible fungi as described in claim 5, characterized in that, The pass-through window (10) is equipped with an ultraviolet disinfection lamp.
7. The integrated sterilization, cooling, and inoculation chamber for edible fungi as described in claim 1, characterized in that, The shoe changing room (1) is equipped with a disinfection cabinet, shoe rack (11), coat rack (12) and handwashing sink.
8. The integrated sterilization, cooling, and inoculation chamber for edible fungi as described in claim 1, characterized in that, The changing room (2) is equipped with a cleanroom wardrobe (13).